CN110029047B - Density gradient preparation device - Google Patents
Density gradient preparation device Download PDFInfo
- Publication number
- CN110029047B CN110029047B CN201910443471.2A CN201910443471A CN110029047B CN 110029047 B CN110029047 B CN 110029047B CN 201910443471 A CN201910443471 A CN 201910443471A CN 110029047 B CN110029047 B CN 110029047B
- Authority
- CN
- China
- Prior art keywords
- fixed
- fixing
- matched
- plate
- box
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 40
- 238000009423 ventilation Methods 0.000 claims description 29
- 238000003825 pressing Methods 0.000 claims description 12
- 230000006835 compression Effects 0.000 claims description 10
- 238000007906 compression Methods 0.000 claims description 10
- 239000000428 dust Substances 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 2
- 210000003463 organelle Anatomy 0.000 description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 7
- 229930006000 Sucrose Natural products 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 239000005720 sucrose Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000005452 bending Methods 0.000 description 2
- 210000003763 chloroplast Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 239000005457 ice water Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 210000003470 mitochondria Anatomy 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 239000002937 thermal insulation foam Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000003793 centrosome Anatomy 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000001085 differential centrifugation Methods 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 210000002288 golgi apparatus Anatomy 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000010627 oxidative phosphorylation Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
Landscapes
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Centrifugal Separators (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a density gradient preparation device which comprises a liquid injector and a centrifuge tube, wherein the liquid injector is matched with the centrifuge tube, the density gradient preparation device further comprises a support frame, a fixing frame and a fixing box, two ends of the fixing frame are in sliding fit with the support frame, a plurality of fixing holes matched with the liquid injector are formed in the fixing frame, the fixing box is arranged below the fixing frame, a support plate is arranged in the middle of the fixing box, a plurality of support holes are formed in the support plate, a mounting plate is obliquely arranged at the bottom of the fixing box, a placement hole is formed in the mounting plate, the centrifuge tube is mounted in the support hole, the bottom of the centrifuge tube is matched with the placement hole, ice cubes are arranged in the fixing box, and the ice cubes are arranged below the support plate. The preparation that can once carry out a plurality of density gradients simultaneously has improved work efficiency greatly, has not only saved the manpower, has alleviateed experimental fatigue, and is fixed more reliable moreover.
Description
Technical Field
The invention relates to the technical field of biological experimental equipment, in particular to a density gradient preparation device.
Background
Organelles are micro-organs with specific morphological structures and functions in the cytoplasm, and the organelles in cells are mainly: mitochondria, endoplasmic reticulum, centrosome, chloroplast, golgi apparatus, ribosome, etc. They constitute the basic structure of the cell, enabling the cell to function properly. Organelles play an important role in maintaining vital activities, such as mitochondria, which are the "dynamic factories" of cells, are the primary sites where cells perform oxidative phosphorylation and synthesize ATP, and are involved in processes such as cell differentiation, cell information transfer, and apoptosis, as well as in regulating cell growth and cell cycle; chloroplasts are organelles unique to plant cells, and are sites where plants perform photosynthesis, and are involved in synthesis of amino acids, fatty acids, plant growth substances, vitamins, and the like, in addition to photosynthesis. Thus, more and more research is going into organelle levels.
The premise behind studying organelles is to isolate highly pure, highly active, morphologically structural integrity organelles. Currently, high purity organelles are isolated mainly by differential centrifugation plus density gradient centrifugation. The medium of density gradient mainly comprises sucrose, glycerol, polyglucose, etc. Each organelle can be isolated because of its different size and sedimentation characteristics. Thus, the preparation of a complete and interface-clear density gradient is a prerequisite for the isolation of high purity organelles. At present, most of the preparation of density gradients is manually prepared, arms cannot be moved greatly in the repeated sucking process of the liquid injector, medium confusion in a centrifuge tube is prevented, arms and cervical vertebrae are easy to fatigue in the whole preparation process, labor intensity is high, only one preparation can be performed at a time, and efficiency is low.
Disclosure of Invention
The invention aims at the defects in the prior art, and provides the density gradient preparation device which can prepare a plurality of density gradients simultaneously at one time, thereby greatly improving the working efficiency, saving the manpower, relieving the experimental fatigue and ensuring more reliable fixation.
In order to achieve the above purpose, the invention provides the following technical scheme:
the utility model provides a density gradient preparation facilities, includes annotates liquid ware, centrifuging tube, annotate liquid ware and centrifuging tube cooperation, still include support frame, fixed box, the both ends of fixed frame with support frame sliding fit, be equipped with on the fixed frame a plurality of with annotate liquid ware complex fixed orifices, the below of fixed frame is located to the fixed box, the middle part of fixed box is equipped with the backup pad, be equipped with a plurality of supporting holes in the backup pad, the bottom slope of fixed box is equipped with the mounting panel, be equipped with on the mounting panel and place the hole, the centrifuging tube is installed in the bottom of supporting hole and centrifuging tube and is placed the hole cooperation, be equipped with the ice-cube in the fixed box, the below of backup pad is located to the ice-cube.
Preferably, the support frame includes bottom plate, bracing piece, connecting rod, the both ends of connecting rod all are equipped with the bracing piece perpendicularly, the lower extreme of backup pad is equipped with the bottom plate, be equipped with the fixed slot on the lateral wall of fixed box, bracing piece and fixed slot cooperation, the both sides of fixed box are located to the bottom plate, the top of fixed box is located to the connecting rod.
Preferably, the fixed frame comprises fixed plates, the fixed orifices are arranged on the fixed plates, the number of the fixed plates is two, the two fixed plates are arranged in parallel, the end parts of the two fixed plates are connected through a connecting plate, one side of the connecting plate is provided with a lifting rod, the end part of the lifting rod is provided with a sliding sleeve, the sliding sleeve is matched with the supporting rod, and a locking bolt used for being connected with the supporting rod is arranged on the sliding sleeve.
Preferably, scale marks are arranged on the side wall of the supporting rod.
Preferably, the side wall of the fixed box is internally provided with heat insulation foam.
Preferably, a supporting plate is arranged on the inner wall of the fixed box, and the supporting plate is arranged at the upper end of the supporting plate.
Preferably, a drain pipe is arranged on the side wall of the fixed box, and a valve is arranged on the drain pipe.
Preferably, the liquid injector comprises a needle cylinder and a needle head, the lower end of the needle cylinder is connected with the needle head, a handle is arranged at the upper end of the side wall of the needle cylinder, a limit ring is arranged at the lower end of the handle, the needle cylinder is matched with the fixing hole, the limit ring is arranged above the fixing hole, and a dust cover is matched with the upper end of the needle cylinder.
Preferably, the shield includes the shield body, the lower extreme of shield body is equipped with the sealing plug, sealing plug and cylinder cooperation, the upper end of shield body is equipped with the hard tube of ventilating with the cylinder intercommunication, and the upper end of hard tube of ventilating is the arc structure, the upper end of hard tube of ventilating even has the breather hose, the tip of breather hose is equipped with air cleaner, the outside cover of breather hose has the velocity of flow regulator.
Preferably, the flow speed regulator comprises a compression bolt and a fixed sleeve, the fixed sleeve is sleeved on the outer side of the ventilation hose, the compression bolt is arranged on the side wall of the fixed sleeve, one end of the compression bolt is provided with a pressing plate, and the pressing plate is arranged in the fixed sleeve.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the plurality of liquid injectors can be placed through the fixing frame, the plurality of centrifuge tubes can be fixed through the fixing box, and the preparation of a plurality of density gradients can be simultaneously carried out at one time, so that the working efficiency is greatly improved.
2. According to the invention, the mounting plate is obliquely arranged at the bottom of the fixing box, the placing hole is formed in the mounting plate, the centrifugal tube is arranged in the supporting hole, the bottom of the centrifugal tube is matched with the placing hole, so that the firmness of the installation of the centrifugal tube is ensured, the inclined arrangement of the centrifugal tube is realized, the liquid flow is more stable when a medium is added, and the confusion of two mediums caused by excessive impact force when the liquid flows down along the inner wall of the centrifugal tube is reduced, so that the preparation is more reliable.
3. The fixing groove is formed in the side wall of the fixing box, the supporting rod is matched with the fixing groove, in use, the matched position of the fixing frame and the fixing box in use can be guaranteed, the complicated adjustment is avoided, the time is saved, the accurate matching of the centrifuge tube and the liquid injector is guaranteed, meanwhile, the supporting frame and the fixing box are prevented from sliding relatively in the use process, and the use safety is improved; the firmness and stability of the support frame in the use process are guaranteed through the bottom plate.
4. According to the invention, the number of the fixing plates is two, the two fixing plates are arranged in parallel, the fixing plates are not only used for placing the liquid injector, but also can play a role in guiding the liquid injector through the lower layer of fixing plates, so that the liquid injector is prevented from shifting in use, and the liquid injector is more stable and reliable in use.
5. According to the invention, the height of the fixed frame is adjusted through the cooperation of the sliding sleeve and the supporting rod, the adjusting mode is simple, the fixed frame is fixed through the locking bolt, the fixed frame is reliable and convenient, the scale marks are arranged on the supporting rod, the consistency of the height adjustment of the two ends of the fixed frame can be ensured, the liquid level in the liquid injector is ensured, and the liquid flow is smoother.
6. According to the invention, the heat-insulating foam is arranged in the side wall of the fixed box, so that the heat-insulating effect can be realized, the melting speed of ice cubes in the fixed box is slowed down, and the cost is saved.
7. The supporting plate is arranged on the inner wall of the fixed box, the supporting plate is arranged at the upper end of the supporting plate, and the supporting plate can support and fix the supporting plate, so that the supporting plate is convenient to assemble and disassemble.
8. According to the invention, the side wall of the fixed box is provided with the drain pipe, and the drain pipe is provided with the valve, so that the ice water in the fixed box can be conveniently discharged, and the convenience of use is improved.
9. According to the invention, the limiting rings are arranged on the side wall of the needle cylinder, so that the position of the needle cylinder can be limited, and the handle is convenient to take the needle cylinder and use.
10. According to the invention, the dustproof cover is arranged on the needle cylinder, impurities in air can be prevented from entering the needle cylinder through the air filter in the preparation process, the air pressure balance in the needle cylinder is ensured through the ventilation hose and the ventilation hard tube, and the smooth fluidity of media is ensured.
11. The upper end of the ventilation hard tube is of the arc-shaped structure, bending between the ventilation hard tube and the ventilation soft tube can be prevented, and air flow is ensured to be normal.
12. The flow rate regulator has simple structure and convenient regulation, and regulates the flow of air entering the syringe by pressing the pressing plate and the ventilation hose, thereby realizing the flow rate regulation of the medium in the syringe, ensuring the medium in the syringe to be added into the centrifuge tube at a moderate speed, and ensuring the successful preparation of the density gradient.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic diagram of the structure of the support frame and the fixing frame;
FIG. 3 is a schematic structural view of the stationary box;
the fixing 4 is a top view of the fixing box after the fixing plate is removed;
FIG. 5 is a schematic view of the structure of the injector;
FIG. 6 is a schematic structural view of a closure cap;
fig. 7 is a schematic structural view of the flow rate regulator.
In the figure: 1-a supporting frame; 101-a bottom plate; 102-supporting rods; 103-graduation marks; 104-connecting rods; 2-a dust cap; 201-an air filter; 202-a breather hose; 203-a flow rate regulator; 204-ventilation hard tube; 205-a dust cap body; 206-sealing plug; 207-pressing bolts; 208-fixing the sleeve; 209-a platen; 3-fixing the frame; 301-fixing holes; 302-a fixed plate; 303-lifting rod; 304-locking bolts; 305-sliding sleeve; 306-connecting plates; 4-a liquid injector; 401-handle; 402-a limiting ring; 403-syringe; 404-needle; 5-a fixed box; 501-insulating foam; 502-a supporting plate; 503-supporting the plate; 504-mounting plate; 505-support holes; 506-valve; 507-drain pipe; 508-a fixed slot; 509-placing holes; 6-ice cubes; 7-centrifuge tube.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1, a density gradient preparation device, including annotating liquid ware 4, centrifuging tube 7, annotate liquid ware 4 and centrifuging tube 7 cooperation, still include support frame 1, fixed frame 3, fixed box 5, fixed frame 3's both ends with support frame 1 sliding fit, be equipped with on the fixed frame 3 a plurality of with annotate liquid ware 4 complex fixed orifices 301, fixed box 5 locates the below of fixed frame 3, fixed box 5's middle part is equipped with backup pad 503, be equipped with a plurality of supporting holes 505 on the backup pad 503, fixed box 5's bottom slope is equipped with mounting panel 504, be equipped with on the mounting panel 504 and place hole 509, centrifuging tube 7 installs in supporting hole 505 and the bottom of centrifuging tube 9 with place hole 509 cooperation, has not only guaranteed the fastness of centrifuging tube 7 installation, has realized moreover that centrifuging tube 7's slope sets up, has guaranteed that liquid flow is more steady when adding the medium, reduces the liquid and flows down too big two kinds of mediums that lead to more reliable preparation. The ice cubes 6 are arranged in the fixed box 5, the ice cubes 6 are arranged below the supporting plate 503, the temperature in the centrifuge tube 7 is kept at about 4 ℃ during preparation through the ice cubes 6, and the completeness of the organelle can be guaranteed only at low temperature, so that breakage is avoided.
As shown in fig. 2, the support frame 1 includes a bottom plate 101, a support rod 102, and a connecting rod 104, two ends of the connecting rod 104 are vertically provided with the support rod 102, a bottom plate 101 is provided at a lower end of the support rod 102, as shown in fig. 3, a fixing groove 508 is provided on a side wall of the fixing box 5, the support rod 102 is matched with the fixing groove 508, the bottom plate 101 is provided at two sides of the fixing box 5, and the connecting rod 104 is provided above the fixing box 5. In use, the matching position of the fixed frame 3 and the fixed box 5 in use can be ensured, the complicated adjustment is avoided, the time is saved, the accurate matching of the centrifuge tube 7 and the liquid injector 4 is ensured, meanwhile, the relative sliding of the support frame 1 and the fixed box 5 in the use process is prevented, and the use safety is increased; the firmness and stability of the support frame 1 during use are ensured by the bottom plate 101.
As shown in fig. 2, the fixing frame 3 includes fixing plates 302, the fixing holes 301 are disposed on the fixing plates 302, two rows of fixing holes 301 are disposed, the number of each row is at least six, the number of the fixing plates 302 is two, the two fixing plates 302 are disposed in parallel, the fixing plates 302 are not only used for placing the liquid injector 4, but also can play a guiding role on the liquid injector 4 through the fixing plates 302 on the lower layer, so as to prevent the offset in use, and the use is more stable and reliable; the ends of the two fixing plates 302 are connected through a connecting plate 306, a lifting rod 303 is arranged on one side of the connecting plate 306, a sliding sleeve 305 is arranged on the end of the lifting rod 303, the sliding sleeve 305 is matched with the supporting rod 102, and a locking bolt 304 used for being connected with the supporting rod 102 is arranged on the sliding sleeve 305. The height adjustment of the fixed frame 3 is realized through the cooperation of the sliding sleeve 305 and the supporting rod 102, the adjustment mode is simple, the fixed is reliable and convenient through the locking bolt 304, the scale mark 103 is arranged on the supporting rod 102, the consistency of the height adjustment of the two ends of the fixed frame 3 can be ensured, the liquid level in the liquid injector 4 is ensured, and the liquid flow is smoother.
As shown in fig. 2, scale marks 103 are provided on the side walls of the support rods 102, so that the height adjustment of the two ends of the fixed frame 3 is consistent, the liquid level in the liquid injector 4 is ensured, and the liquid flow is smoother.
As shown in fig. 1, the side wall of the fixed box 5 is provided with heat insulation foam 501, which can play a role in heat insulation, slow down the melting speed of the ice cubes 6 in the fixed box 5 and save the cost.
As shown in fig. 1 and 4, in order to facilitate the assembly and disassembly of the support plate 503, a pallet 502 is provided on the inner wall of the stationary box 5, and the support plate 503 is mounted on the upper end of the pallet 502.
As shown in fig. 3, the side wall of the fixed tank 5 is provided with a drain pipe 507, and the drain pipe 507 is provided with a valve 506, so that the ice water in the fixed tank 5 can be conveniently discharged, and the convenience of use is improved.
As shown in fig. 5, the liquid injector 4 comprises a syringe 403 and a needle 404, the lower end of the syringe 403 is connected with the needle 404, the upper end of the side wall of the syringe 403 is provided with a handle 401, the lower end of the handle 401 is provided with a limit ring 402, the syringe 403 is matched with the fixing hole 301, the limit ring 402 is arranged above the fixing hole 301, the limit ring 402 can be used for mounting and limiting the position of the syringe 403, and the handle 401 is convenient for taking the syringe 403 and is convenient for use.
As shown in fig. 1, the upper end of the syringe 403 is matched with a dust cover 2, as shown in fig. 6, the dust cover 2 includes a dust cover body 205, a sealing plug 206 is disposed at the lower end of the dust cover body 205, a hard ventilation tube 204 is disposed at the upper end of the dust cover body 205 and is communicated with the syringe 403, the upper end of the hard ventilation tube 204 has an arc structure, which plays a transitional role, bending between the hard ventilation tube 204 and the hard ventilation tube 202 can be prevented, normal air flow is ensured, the upper end of the hard ventilation tube 204 is connected with the soft ventilation tube 202, an air filter 201 is disposed at the end of the soft ventilation tube 202, the air filter 201 is the same as the air filter used in the infusion apparatus, and the structure is not described in detail, a flow rate regulator 203 is sleeved at the outer side of the soft ventilation tube 202, impurities in air can be prevented from entering the syringe 403 in the preparation process, air pressure balance in the syringe 403 is ensured through the soft ventilation tube 202 and the hard ventilation tube 204, and smooth fluidity of media is ensured.
As shown in fig. 7, the flow rate regulator 203 includes a compression bolt 207 and a fixing sleeve 208, the fixing sleeve 208 is sleeved on the outer side of the ventilation hose 202, the compression bolt 207 is disposed on the side wall of the fixing sleeve 208, one end of the compression bolt 207 is provided with a pressing plate 209, the pressing plate 209 is disposed in the fixing sleeve 208, the pressing plate 209 is pressed on the side wall of the ventilation hose 202, the extrusion degree of the pressing plate 209 to the ventilation hose 202 can be regulated by rotating the compression bolt 207, the flow rate regulator 203 is simple in structure and convenient to regulate, the flow rate of air entering the syringe 403 is regulated by the pressing plate 209 and the ventilation hose 202, so that the flow rate regulation of media in the syringe 403 is realized, the medium adding speed in the centrifuge tube 7 is ensured, and the successful preparation of the density gradient is ensured.
In use, as shown in fig. 1, before the density gradient is prepared, the support frame 1 is first connected with the fixing box 5, so that the support rod 102 is matched with the fixing groove 508, two mediums with different densities are then prepared, in this embodiment, sucrose mediums are used, one part of the mediums is 40% and the other part of mediums is 20%, firstly, the support plate 503 is opened, ice cubes 6 are added into the fixing box 5, then the support plate 503 is put back into the fixing box 5, 40% of sucrose is added into the centrifuge tube 7, approximately one third of the position of the centrifuge tube 7 is added, then the centrifuge tube 7 is placed into the support hole 505, the lower end of the centrifuge tube 7 is matched with the placing hole 509, the centrifuge tube 7 is inclined against the support hole 505, 20% of sucrose is added into the syringe 403, the dust cover 2 is matched with the syringe 403, meanwhile, the flow rate regulator 203 is closed, the syringe 403 is placed into the fixing hole 301, the height of the needle 404 is adjusted up and down into the centrifuge tube 7, meanwhile, the inner wall of the ice cubes 6 is added into the centrifuge tube 5, then the support plate 503 is put back into the fixing box 5, 40% of sucrose is added into the centrifuge tube 7, approximately one third of the centrifuge tube 7 is added into the centrifuge tube through the locking bolt 304, 20% of the centrifuge tube is pressed down, 20% of the sucrose is adjusted, and 20% of the sucrose is gradually added into the centrifuge tube along the position of the inner wall of the centrifuge tube 7, 20% of the position of the centrifuge tube is gradually, 20% of the position of the inner wall of the layer is gradually added into the centrifuge tube, and 20% of the layer, 20% of the layer is gradually reached, and 20% of the layer is gradually reached. Can place a plurality of annotates liquid ware 4 through fixed frame 3, fixed box 5 fixes a plurality of centrifuging tubes 7, can once carry out the preparation of a plurality of density gradients simultaneously, work efficiency has been improved greatly, through the device in the preparation process, the staff need not hold annotate liquid ware 4 any more, not only saved the manpower, the experiment fatigue has been alleviateed, it is more reliable to fix moreover, can not take place to rock when whole filling medium, avoid the confusion of medium in centrifuging tube 7, the smooth of preparation has been ensured, the preparation failure times has been reduced, save the material, let the experiment more reliable.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (2)
1. The utility model provides a density gradient preparation facilities, includes annotates liquid ware, centrifuging tube, annotate liquid ware and centrifuging tube cooperation, its characterized in that: the centrifugal tube is arranged in the supporting holes, the bottom of the centrifugal tube is matched with the placing holes, ice cubes are arranged in the fixing box, the lower part of the supporting plate is arranged below the fixing box, a supporting plate is arranged on the middle of the fixing box, a plurality of supporting holes are formed in the supporting plate, a water draining pipe is arranged on the side wall of the fixing box, and a valve is arranged on the water draining pipe;
the support frame comprises a bottom plate, support rods and connecting rods, wherein the support rods are vertically arranged at two ends of the connecting rods, the bottom plate is arranged at the lower end of the support plate, a fixed groove is formed in the side wall of the fixed box, the support rods are matched with the fixed groove, the bottom plate is arranged at two sides of the fixed box, and the connecting rods are arranged above the fixed box;
the liquid injector comprises a needle cylinder and a needle head, wherein the lower end of the needle cylinder is connected with the needle head, the upper end of the side wall of the needle cylinder is provided with a handle, the lower end of the handle is provided with a limiting ring, the needle cylinder is matched with a fixing hole, the limiting ring is arranged above the fixing hole, and the upper end of the needle cylinder is matched with a dust cover; the dustproof cover comprises a dustproof cover body, wherein a sealing plug is arranged at the lower end of the dustproof cover body and matched with the needle cylinder, a ventilation hard tube communicated with the needle cylinder is arranged at the upper end of the dustproof cover body, the upper end of the ventilation hard tube is of an arc-shaped structure, a ventilation hose is connected with the upper end of the ventilation hard tube, an air filter is arranged at the end part of the ventilation hose, and a flow speed regulator is sleeved on the outer side of the ventilation hose; the flow speed regulator comprises a compression bolt and a fixed sleeve, the fixed sleeve is sleeved on the outer side of the ventilation hose, the side wall of the fixed sleeve is provided with the compression bolt, one end of the compression bolt is provided with a pressing plate, and the pressing plate is arranged in the fixed sleeve;
the fixed frame comprises fixed plates, the fixed orifices are arranged on the fixed plates, the fixed plates are two in number and are arranged in parallel, the end parts of the two fixed plates are connected through a connecting plate, one side of the connecting plate is provided with a lifting rod, the end part of the lifting rod is provided with a sliding sleeve, the sliding sleeve is matched with a supporting rod, and a locking bolt used for being connected with the supporting rod is arranged on the sliding sleeve.
2. A density gradient producing apparatus as claimed in claim 1, wherein: scale marks are arranged on the side wall of the supporting rod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910443471.2A CN110029047B (en) | 2019-05-24 | 2019-05-24 | Density gradient preparation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910443471.2A CN110029047B (en) | 2019-05-24 | 2019-05-24 | Density gradient preparation device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110029047A CN110029047A (en) | 2019-07-19 |
| CN110029047B true CN110029047B (en) | 2024-03-29 |
Family
ID=67243464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910443471.2A Active CN110029047B (en) | 2019-05-24 | 2019-05-24 | Density gradient preparation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110029047B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2691611Y (en) * | 2004-03-08 | 2005-04-13 | 汤福广 | Three purpose discharging adding transfusion system |
| CN201823546U (en) * | 2010-10-27 | 2011-05-11 | 西北农林科技大学 | Novel automatic suction-discharge type calibrated suction pipe capable of preventing excessive suction |
| CN202366739U (en) * | 2011-12-08 | 2012-08-08 | 梁春 | Pipe frame support structure |
| CN203264746U (en) * | 2013-05-03 | 2013-11-06 | 苏州杰诺曼博生物科技有限公司 | Multi-channel liquid sample injector |
| CN203342824U (en) * | 2013-06-23 | 2013-12-18 | 张婷英 | Medical liquid extractor with pump pipe |
| CN104549592A (en) * | 2013-10-25 | 2015-04-29 | 大连民族学院 | Pipette |
| CN210001858U (en) * | 2019-05-24 | 2020-01-31 | 山东省农作物种质资源中心 | density gradient preparation facilities |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10391249B2 (en) * | 2017-01-30 | 2019-08-27 | Belal Hassanzadeh | Dropper syringe apparatus |
-
2019
- 2019-05-24 CN CN201910443471.2A patent/CN110029047B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2691611Y (en) * | 2004-03-08 | 2005-04-13 | 汤福广 | Three purpose discharging adding transfusion system |
| CN201823546U (en) * | 2010-10-27 | 2011-05-11 | 西北农林科技大学 | Novel automatic suction-discharge type calibrated suction pipe capable of preventing excessive suction |
| CN202366739U (en) * | 2011-12-08 | 2012-08-08 | 梁春 | Pipe frame support structure |
| CN203264746U (en) * | 2013-05-03 | 2013-11-06 | 苏州杰诺曼博生物科技有限公司 | Multi-channel liquid sample injector |
| CN203342824U (en) * | 2013-06-23 | 2013-12-18 | 张婷英 | Medical liquid extractor with pump pipe |
| CN104549592A (en) * | 2013-10-25 | 2015-04-29 | 大连民族学院 | Pipette |
| CN210001858U (en) * | 2019-05-24 | 2020-01-31 | 山东省农作物种质资源中心 | density gradient preparation facilities |
Non-Patent Citations (1)
| Title |
|---|
| 离心分析用蔗糖密度梯度简易制备法;夏邦颖;动物学研究(第04期);第559-562页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110029047A (en) | 2019-07-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110029047B (en) | Density gradient preparation device | |
| CN210001858U (en) | density gradient preparation facilities | |
| CN209234570U (en) | A kind of soilless cultivating flowers device | |
| CN208798396U (en) | A kind of A type frame culture apparatus | |
| CN207987246U (en) | A kind of biological perfusion reaction system applied to bone tissue engineer micro-assembly robot culture | |
| CN208213870U (en) | A kind of box for breeding cleaning plant used for aquiculture | |
| CN209628304U (en) | A kind of soilless cultivation mechanism | |
| CN210352552U (en) | Dendrobium officinale tissue culture inoculation equipment | |
| CN112553073A (en) | Composite culture tank for multi-cell culture | |
| CN217284376U (en) | Nutrient solution self-circulation water culture device | |
| CN208776742U (en) | Medium bottle for stem cell | |
| CN207803084U (en) | Tissue-cultured seedling culture of rootage device | |
| CN207692628U (en) | A kind of potato culture field planting plate | |
| CN217284381U (en) | Tissue culture case that plant tissue culture used | |
| CN205596689U (en) | Frame is cultivateed to convertible plant | |
| CN218932183U (en) | Portable portable cell incubator | |
| CN214338837U (en) | Urban and rural construction planting technique building block | |
| CN205838514U (en) | A kind of half tamponade medicine bottle total head plug mechanism | |
| CN214971787U (en) | Device for adjusting air pressure of drug concentration inner cavity | |
| CN213548891U (en) | Novel large-size water dropper | |
| CN214496351U (en) | Medicine cell culture device | |
| CN210999667U (en) | Integral hot vulcanization rubber lining equipment for large desulfurization butterfly valve of power plant | |
| CN219612752U (en) | Culture flask | |
| CN208029639U (en) | Plant growth disk | |
| CN208034937U (en) | A kind of bamboo plate production bamboo particle spray unit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |