CN208776742U - Medium bottle for stem cell - Google Patents
Medium bottle for stem cell Download PDFInfo
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- CN208776742U CN208776742U CN201821109110.1U CN201821109110U CN208776742U CN 208776742 U CN208776742 U CN 208776742U CN 201821109110 U CN201821109110 U CN 201821109110U CN 208776742 U CN208776742 U CN 208776742U
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- bottle
- stem cell
- substrate
- medium bottle
- step ring
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Abstract
The utility model discloses a kind of medium bottles for stem cell, including culture medium bottle body, the bottom end of the culture medium bottle body is fixedly connected with pedestal, bottle chamber is equipped in the medium bottle body, the top of the culture medium bottle body is connected with bottle cap, the center bottom of the bottle cap is connected with bottle stopper, the side wall of the bottle chamber is successively fixed with multiple step rings from bottom to top, the size of the step ring is sequentially increased from bottom to top and the spacing of two neighboring step ring is consistent, the top of the step ring is equipped with substrate, the top two sides of the substrate are fixedly connected to pull ring, the bottom end of the substrate is fixedly connected to connecting plate, the bottom end of the connecting plate is respectively and fixedly provided with four fixed columns.The utility model can effectively increase the surface area of stem cell attachment and growth, the survival rate of stem cell be improved, while the installing and dismounting operation of bottle cap, substrate is convenient, conducive to the culture of stem cell.
Description
Technical field
The utility model relates to technical field of cell culture more particularly to a kind of medium bottles for stem cell.
Background technique
Stem cell is a kind of from tissue, can be proliferated and can be with the cell of Multidirectional Differentiation.It has multi-party
The characteristics of face, including break up to various kinds of cell, immunological regulation, increase blood supply etc..And existing culture eukaryocyte is most
Normal method needs large capacity incubator, needs labor intensive operations culture bottle, and the time for being inoculated with and cultivating compared with
It is long, it involves great expense and unfavorable to cell survival.
Through retrieving, Chinese patent grant number CN206538439U discloses a kind of stem cell media bottle, including base bottle sheet
Body, bottleneck, bottleneck and bottle stopper, the bottom of the base bottle body are equipped with pedestal, removably configured with multiple on the pedestal
Substrate, the substrate are connect by connector with the pedestal, and the size for being dimensioned slightly smaller than the pedestal of the substrate is described
The shape of substrate is identical with the shape of pedestal, and appropriately distance is kept between the multiple substrate, is also equipped with training in the base bottle
Support base.
The existing medium bottle for stem cell has the following disadvantages: culture area is smaller with space, so that dry
Cell culture difficulty increases, and is unfavorable for improving the survival rate of stem cell, while substrate replacement operation is cumbersome, is unfavorable for stem cell
Culture.
Utility model content
The purpose of this utility model is to provide a kind of medium bottle for stem cell, have can effectively increase it is dry
The surface area of cell attachment and growth improves the survival rate of stem cell, while the installing and dismounting operation of bottle cap, substrate is convenient, benefit
In the stem cell culture the advantages of, it is smaller with space to solve existing apparatus culture area, so that stem cell culture difficulty increase, no
The problem of conducive to the survival rate for improving stem cell, while substrate replacement operation is cumbersome, is unfavorable for stem cell culture.
According to a kind of medium bottle for stem cell of the utility model embodiment, including culture medium bottle body, the training
The bottom end for supporting base bottle body is fixedly connected with pedestal, and bottle chamber is equipped in the medium bottle body, and the top of the culture medium bottle body connects
It is connected to bottle cap, the center bottom of the bottle cap is connected with bottle stopper, and the side wall of the bottle chamber is successively fixed with multiple from bottom to top
The size of rank ring, the step ring is sequentially increased from bottom to top and the spacing of two neighboring step ring is consistent, the step ring
Top is equipped with substrate, and the top two sides of the substrate are fixedly connected to pull ring, and the bottom end of the substrate is fixedly connected to
Connecting plate, the bottom end of the connecting plate are respectively and fixedly provided with four fixed columns, and the fixed column protrudes into the fixation hole of step ring, institute
The external wall of upper portion for stating fixed column is respectively and fixedly provided with sealing ring, and the outer wall of the sealing ring offsets with fixation hole inner wall.
Further, the culture medium bottle body is in rounding mesa-shaped, and the side wall of the step ring is oblique.
Further, the diameter of substrate described in lower layer is less than the internal diameter of upper step ring.
Further, four fixation holes are symmetrical set about the center of step ring.
Further, the quantity of the step ring is 5-8.
Further, the top of the bottle cap is fixedly connected with handle.
The utility model has the beneficial effect that compared with prior art
1, by the way that stem cell attachment and life can be made using step ring supporting substrate in the intracavitary multiple step rings of setting of bottle
Long surface area increases, then supports the environment of stem cell growth in the intracavitary configuration of bottle, must can effectively improve the survival of stem cell
Rate reduces culture difficulty；
2, bottle cap can easily be dismantled by handle with bottleneck, and substrate passes through consolidating in pull ring, fixed column and step ring
Determine hole connection, installation operation is convenient, while pedestal can play buffer action, prevents external environment from influencing the intracavitary environment of bottle, just
In the culture of stem cell.
Detailed description of the invention
Attached drawing is used to provide a further understanding of the present invention, and constitutes part of specification, practical with this
Novel embodiment is used to explain the utility model together, does not constitute limitations of the present invention.In the accompanying drawings:
Fig. 1 be the utility model proposes a kind of medium bottle for stem cell structural schematic diagram；
Fig. 2 is the structure enlargement diagram of a-quadrant in Fig. 1；
Fig. 3 be the utility model proposes a kind of medium bottle for stem cell step ring structure schematic diagram.
In figure: 1- culture medium bottle body, 2- handle, 3- bottles of chambers, 4- pull ring, 5- bottle cap, 6- pedestal, 7- step ring, 8- substrate,
9- connecting plate, 10- fixed column, 11- fixation hole, 12- sealing ring, 13- bottle stopper.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Referring to Fig.1-3, a kind of medium bottle for stem cell, including culture medium bottle body 1, the bottom end of culture medium bottle body 1
It is fixedly connected with pedestal 6, is made of barrier material, buffer action can be played, prevents working face that may be moist from influencing training
The problem of supporting environment in base bottle body 1, culture medium bottle body 1 is interior to be equipped with bottle chamber 3, and bottle chamber 3 is provided with culture medium, the life for stem cell
Long, the top of culture medium bottle body 1 is connected with bottle cap 5, and bottle cap 5 and the connection of culture medium bottle body 1 are removable, the center bottom of bottle cap 5
Be connected with bottle stopper 13, the side wall of bottle chamber 3 is successively fixed with multiple step rings 7 from bottom to top, the size of step ring 7 from bottom to top according to
The spacing of secondary increase and two neighboring step ring 7 is consistent, and the top of step ring 7 is equipped with substrate 8, and the top two sides of substrate 8 are equal
It is fixedly connected with pull ring 4, convenient for taking out substrate 8, substrate 8 is already subjected to plasma surface treatment, forms the material of substrate 8 extremely
It include less one of polyamide, polycarbonate, polysulfones, polyacetals and polyvinyl chloride, the bottom end of substrate 8 is fixedly connected to company
Fishplate bar 9, the bottom end of connecting plate 9 are respectively and fixedly provided with four fixed columns 10, and fixed column 10 protrudes into the fixation hole 11 of step ring 7, Gu
Determine the top that hole 11 is located at step ring 7, the external wall of upper portion of fixed column 10 is respectively and fixedly provided with sealing ring 12, the outer wall of sealing ring 12 with
11 inner wall of fixation hole offsets, so that the installation of substrate 8 is more convenient, convenient for the maintenance and replacement in later period.
Culture medium bottle body 1 is in rounding mesa-shaped, and the side wall of step ring 7 is oblique；The diameter of underlying substrate 8 is less than top
The internal diameter of step ring 7, so that the taking-up of substrate 8 is more convenient；Four fixation holes 11 are in symmetrically to set about the center of step ring 7
It sets, uniform force；The quantity of step ring 7 is 5-8；The top of bottle cap 5 is fixedly connected with handle 2, convenient for the fractionation of bottle cap 5.
In use, culture medium bottle body 1 is placed on pedestal 6, is then applied on the inner wall of substrate 8 and bottle chamber 3 and contain albumen
The coating of matter and polylysine can promote the attachment and growth of stem cell, be fixed on fixed column 10 in fixation hole 11,
Sealing ring 12 completes the top seal of fixation hole 11, is put into stem cell and culture medium inside bottle chamber 3, can carry out the training of stem cell
It supports, the substrate 8 on the step ring 7 of multilayer makes the apposition growth area of stem cell increase with round table-like culture medium bottle body 1
Add, so that the survival rate of stem cell can be improved.
Place is not described in detail for the utility model, is the well-known technique of those skilled in the art.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of medium bottle for stem cell, it is characterised in that: including culture medium bottle body (1), the culture medium bottle body (1)
Bottom end be fixedly connected with pedestal (6), be equipped with bottle chamber (3) in the culture medium bottle body (1), the top of the culture medium bottle body (1)
End is connected with bottle cap (5), and the center bottom of the bottle cap (5) is connected with bottle stopper (13), and the side wall of the bottle chamber (3) is from bottom to top
Multiple step rings (7) are successively fixed with, the size of the step ring (7) is sequentially increased from bottom to top and two neighboring step ring
(7) spacing is consistent, and the top of the step ring (7) is equipped with substrate (8), and the top two sides of the substrate (8) are fixed to be connected
It is connected to pull ring (4), the bottom end of the substrate (8) is fixedly connected to connecting plate (9), and the bottom end of the connecting plate (9) is fixed
There are four fixed column (10), the fixed column (10) is protruded into the fixation hole (11) of step ring (7), the fixed column (10)
External wall of upper portion is respectively and fixedly provided with sealing ring (12), and the outer wall of the sealing ring (12) offsets with fixation hole (11) inner wall.
2. a kind of medium bottle for stem cell according to claim 1, it is characterised in that: the culture medium bottle body
It (1) is in rounding mesa-shaped, the side wall of the step ring (7) is oblique.
3. a kind of medium bottle for stem cell according to claim 1, it is characterised in that: substrate described in lower layer (8)
Diameter be less than upper step ring (7) internal diameter.
4. a kind of medium bottle for stem cell according to claim 1, it is characterised in that: four fixation holes
(11) center about step ring (7) is symmetrical set.
5. a kind of medium bottle for stem cell according to claim 1, it is characterised in that: the step ring (7)
Quantity is 5-8.
6. a kind of medium bottle for stem cell according to claim 1, it is characterised in that: the top of the bottle cap (5)
End is fixedly connected with handle (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821109110.1U CN208776742U (en) | 2018-07-12 | 2018-07-12 | Medium bottle for stem cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821109110.1U CN208776742U (en) | 2018-07-12 | 2018-07-12 | Medium bottle for stem cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208776742U true CN208776742U (en) | 2019-04-23 |
Family
ID=66153488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821109110.1U Active CN208776742U (en) | 2018-07-12 | 2018-07-12 | Medium bottle for stem cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208776742U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113583875A (en) * | 2021-08-19 | 2021-11-02 | 生物角（厦门）科技有限公司 | Biological culture microcarrier and application thereof in biological culture system |
-
2018
- 2018-07-12 CN CN201821109110.1U patent/CN208776742U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113583875A (en) * | 2021-08-19 | 2021-11-02 | 生物角（厦门）科技有限公司 | Biological culture microcarrier and application thereof in biological culture system |
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