CN109999144A - It is a kind of for preventing and treating the herbal mixture fermentation preparation of hydropsy for baby pigs - Google Patents
It is a kind of for preventing and treating the herbal mixture fermentation preparation of hydropsy for baby pigs Download PDFInfo
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Abstract
The present invention relates to a kind of for preventing and treating the Chinese medicine composition of hydropsy for baby pigs, it is the medicinal material following mass fraction through medicament made of microbial fermentation: 15~30 parts of radix scutellariae, 15~30 parts of Radix Isatidis, 12~20 parts of Poria cocos, 12~20 parts of rde bean, 12~20 parts of corn stigma, 8~18 parts of Radix Astragali, 8~18 parts of ramulus cinnamomi, 8~18 parts of dried orange peel, 5~12 parts of Radix Glycyrrhizae.The traditional chinese medicine composition of the invention is after water mentions, with probiotic mixed fermentation, the probiotics herbal mixture fermentation liquid for the hydropsy for baby pigs that obtains medical treatment；The performance of drug effect and the feeding cost of saving Chinese medicaments can be improved in the advantage that this product can play probiotics again, it is green, safely, effectively Substitutes For Antibiotic, there is preferable control efficiency to hydropsy for baby pigs caused by bacterium infection, have the function of clearing heat and detoxicating, antibacterial bacteriostatic, anti-inflammation detumescence, heart tonifying diuresis, strengthen immunity.The features such as present invention is simple with preparation process, curative effect is preferable, has no drug resistance, drug residue free, safe ready.
Description
Technical field
The invention belongs to field of veterinary medicine preparation, and in particular to a kind of herbal mixture fermentation system for preventing and treating hydropsy for baby pigs
Agent.
Background technique
Hydropsy for baby pigs also known as pig stomach intestinal edema are a kind of urgency for causing weanling pig by the toxin of hemolytic relative gene
Property, sporadic, fatal disease.When the decline of piglet resistance, enteral hemolytic relative gene mass propagation can be made and generated
Toxin and cause acute enterotoxemia, be mainly shown as sudden onset, locomotor ataxia is fainted from fear, locally or systemically paralysis and
Cephalledema.The oedema of dissect variation film between subcutaneous, stomach wall and large intestine for head.It is mainly in the spring and autumn sows farrowing phase, it is main
If 1~2 monthly age, the delactational piglets that weight is 7.5~25kg are easily sent out, morbidity is common in 5~15d after ablactation.In recent years, piglet
The disease incidence of oedema is very high, sometimes up to 20%~50%, and big age in days of falling ill (2.5~3 monthly ages, weight are 30~
40kg), common in the form of group's outburst, accidental individually to distribute, it is eutrophy and physically fit piglet, the death rate that morbidity is mostly
Also higher, huge economic loss is caused to pig breeding industry.
Hemolytic relative gene has between Piglet at Lactation Period to be present in enteron aisle on a small quantity.Served as early-weaning, feed mutation,
Feed excessive concentrated feed, be deficient in vitamin E and selenium, environment change, vaccine inoculation, abrupt change of climate, Abwehrkraft des Koepers decline and mother
After one or more of inducements such as source antibody disappearance occur, piglet gastrointestinal dysfunction, enteral microecological balance is destroyed, and is promoted molten
Hemorrhagic Escherichia coli abnormal mass propagation in enteron aisle, generates and causes edema toxin and diarrhea toxin.Two kinds of toxin are through intestinal absorption
It is gradually gathered in vivo after into blood, acts on nervous centralis and blood vessel, cause the diseases such as vasopermeability increase, tissue edema
Change and diarrhea, nervous symptoms eventually lead to piglet death.
Edema disease of piglets clinical manifestation can be summarized as following 4 kinds:
Most acute: sudden onset collapses in the ground, whole-body muscle and tic of limbs, slobbering, saliva, and breathing is extremely difficult,
It is dead rapidly, the course of disease only more than ten minutes to 2 h.
Acute: disease time is very short, and appetite abolish is walked haltingly on foot, arrested by force, and muscular tremor croons, and faints from fear, expiratory dyspnea；
Eyelid Severe edema, incidence oedema；Have sharp ears, nose cyanosis；It goes hither and thither, it is i.e. dead soon after falling down to the ground.2~24 h of the course of disease.
Subacute: morbidity is very fast, loss of appetite, and most body temperature increase (40~41 DEG C)；Walking incoordination, rear quarters shake
Pendulum；Palpebral edema is obvious, conjunctiva flush；Most pigs have diarrhea (occasionally having constipation)；Skin is more sensitive.To the later period, four limbs after falling down to the ground
In swimming shape, hypothermia, skin cyanosis under have sharp ears, nose, abdomen, expiratory dyspnea, failure and die.General 2~6 d of the course of disease.
Chronic: morbidity is slower, and initial symptoms are unobvious.Majority, which has, has loose bowels, and excrement is in celadon more.It is sick that just only conjunctiva is damp
Red, the extremely slight edema of eyelid, body temperature occasionally has raising, and appetite is declined slightly.There is obvious respiratory symptom in later period, and eyelet is rubescent, and happiness is sleeping,
Occasionally there is vomiting, generate heat, constipation is dead after several weeks or become cad pig.
Currently, there is no the therapeutic agent of special efficacy to hydropsy for baby pigs, clinically use antibiotic (Enrofloxacin, strength more
Mycin etc.) it is prevented and treated and is treated, antibiotic easily remains in animal body, generates drug resistance and side effect is big, and drug therapy
Early stage results are good, and the later period is substantially invalid.It is an object of that present invention to provide a kind of reasonable recipe, preparation is simple, can effectively prevent
The herbal mixture fermentation preparation of hydropsy for baby pigs not only can be effectively prevented hydropsy for baby pigs, be mentioned with rapid recovery clinical symptoms
High immunity of organisms, and it is highly-safe, have no drug resistance, drug residue free.
Summary of the invention
The technical problem to be solved by the present invention is to be to provide a kind of herbal mixture hair for preventing and treating hydropsy for baby pigs
Herbal mixture fermentation liquid is made after water mentions, with probiotic mixed fermentation in ferment preparation, Chinese medicine composition, and this product can play benefit
The feeding cost of drug effect performance and saving Chinese medicaments, piglet caused by infecting hemolytic relative gene can be improved in the advantage of raw bacterium again
Oedema, enterotoxemia have significant therapeutic effect, and prolonged application noresidue, without drug resistance, green, safety, user is easy
It absorbs.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
It is a kind of for preventing and treating the Chinese medicine composition of hydropsy for baby pigs, it is composed of the following components: radix scutellariae, Radix Isatidis, Poria cocos, red small
Beans, corn stigma, Radix Astragali, ramulus cinnamomi, dried orange peel, Radix Glycyrrhizae.
Each component mass fraction is respectively as follows: 15~30 parts of radix scutellariae, 15~30 parts of Radix Isatidis, 12~20 parts of Poria cocos, red small
12~20 parts of beans, 12~20 parts of corn stigma, 8~18 parts of Radix Astragali, 8~18 parts of ramulus cinnamomi, 8~18 parts of dried orange peel, Radix Glycyrrhizae 5~12
Part.
The present invention also provides a kind of for preventing and treating the herbal mixture fermentation preparation of hydropsy for baby pigs, by Chinese medicine composition with
Probiotics is made in 100:3 ratio mixed fermentation.
The probiotics is one or more of saccharomycete, Bifidobacterium, lactic acid bacteria, bacillus subtilis.
The pharmacological action of each taste Chinese medicine of the present invention is as follows:
Radix scutellariae: there is heat-clearing and damp-drying drug, purging fire for removing toxin, diuresis, sedation；Radix scutellariae mainly contains scutelloside, wogonoside, radix scutellariae
The flavone compounds such as element play antibacterial, anti-inflammatory, antianaphylactic.In document, radix scutellariae is common in treatment chicken colibacillosis
In prescription agent.
Radix Isatidis: clearing heat and detoxicating, cool blood, relieving sore-throat.With antibacterial, antivirus action.Open the researchs such as text report, lactic acid bacteria
Can be grown in the traditional Chinese medicine of the five flavours such as Radix Isatidis, it is fermented after medical fluid to Escherichia coli, salmonella and Staphylococcus aureus
Bacterium has significant fungistatic effect.
Poria cocos: clearing damp and promoting diuresis, strengthening the spleen and stomach, calming heart enhance body's immunity, adjust gastrointestinal function, and liver protection is calm, main
It harnesses the river wet turgor, difficult urination.Section is superfine the study found that after lactobacillus-fermented Poria cocos, and the content of pachymaran is aobvious in fermentation liquid
It writes and increases, pachymaran has certain scavenging effect to ultra-oxygen anion free radical, hydroxy radical, has compared with strong antioxidant action.
Rde bean: having antibacterial, diuresis, and drying damp and strengthening spleen, inducing diuresis for removing edema, edema swelling, carbuncle swells temperature can apply,
And protein and amino acid rich in red bean.Dong Yingli etc. can improve red bean the study found that lactobacillus-fermented red bean cream
The content of middle polyphenols, polyphenol have potential sanatory effect.
Corn stigma: it is sweet in flavor mild-natured, there is good diuresis, expel the heat-evil, calming the liver pneumostatic, cholagogue, detumescence effect, it is scorching to be usually used in treatment
Disease oedema, stomach and intestine lesion.Vitamin, minerals, amino acid, polysaccharide etc., the happy plum research hair of Ji Lilian, model are rich in corn stigma
Existing, after lactobacillus-fermented, the content of amino acid and minerals dramatically increases corn stigma juice.
Radix Astragali, Radix Astragali are a kind of common righting qi-invigorating herbs, are integration of drinking and medicinal herbs.Radix Astragali has enhancing immunity of organism
The pharmacological activity such as power, tonifying Qi and lifting yang, liver protection is anti-inflammatory, decompression of reinforcing the kidney, inducing diuresis for removing edema, antitumor, antibacterial, main component are Radix Astragali
Polysaccharide, Zhu Xinshu etc. the study found that lactobacillus-fermented Radix Astragali, make astragalus polyose slightly proposing rate improves 111.9%, in zymophyte
During growth metabolism, a series of enzyme systems can be generated, Large molecule active mass degradation in Radix Astragali is converted to and is easy to absorb benefit
Active material improves the bioactivity of Radix Astragali.
Ramulus cinnamomi: there is sweating expelling pathogenic factors from muscles and skin, warming meridian, helps yang transforming qi, diuresis cough-relieving, antibacterial action.
Dried orange peel: have qi-regulating stomach invigorating, eliminating dampness and eliminating phlegm, stopping nausea and vomiting by lowering the adverse flow of QI, dispersing stagnated hepatoqi, promote digestion, promote immune function, it is anti-inflammatory,
The effects of anti-oxidant.Wang Jianguo etc. the study found that supernatant of the dried orange peel after lactobacillus-fermented to Escherichia coli, salmonella,
Staphylococcus aureus all has significant fungistatic effect.
Radix Glycyrrhizae: it is sweet in flavor, it is mild-natured, have and invigorates the spleen and benefits qi, clearing heat and detoxicating, expelling phlegm and arresting coughing, invigorating spleen function.Radix Glycyrrhizae is mild-natured
With, it can and can delay, solve but also mend not only, the function with mediation property of medicine.It is equal the study found that after lactobacillus reuteri fermentation Radix Glycyrrhizae to open text
Supernatant to Escherichia coli have significant fungistatic effect.
Composition principle of the present invention is: Fang Zhong, radix scutellariae, heat-clearing and damp-drying drug, the key medicine of purging fire for removing toxin, and there is antimicrobial antiphlogistic to make
With the effect of, Radix Isatidis is clearing heat and detoxicating, cool blood, antibacterial, antiviral, and clearing heat and detoxicating total spectrum, antibacterial is used in conjunction in the two.Rde bean can be clear
Thermal detoxification, Poria cocos are good at eliminating dampness and heat, and corn stigma detumescence removes inflammation, and Poria cocos, rde bean, corn stigma all have the function of clearing damp and promoting diuresis
Effect, normal compatible use make to let out and reduce swelling under water-wet, may advantageously facilitate the discharge of sick pig internal water.Ramulus cinnamomi spasmolysis heart tonifying, sweating
Expelling pathogenic factors from muscles and skin, Wen Jing activate yang, and body fluid can be helped unobstructed, can temperature help spleen sun to help Yun Shui, and can warm kidney sun, bladder gas is helped by cold-evil
Change.Ramulus cinnamomi obtains Poria cocos specially in changing gas row water, and Poria cocos obtains ramulus cinnamomi and then activates yang dehumidifying, and sharp waterpower is strong.Radix Astragali have replenish qi to invigorate the spleen, heart tonifying
Calm the nerves, function, then strengthening the spleen and replenishing qi is used in conjunction with Poria cocos and reinforces, and immunity of organisms can be improved.Dried orange peel enters spleen, lung channel, liver-smoothing, qi-regulating
Invigorating the spleen, eliminating dampness and eliminating phlegm, then tonifying lung is used in conjunction with Radix Glycyrrhizae in antimicrobial antiphlogistic effect.Radix Glycyrrhizae property sweet and neutral plays the role of mediation property of medicine, both may be used
The property that other drugs poison is strong in formula is reduced, and can be relieved and gastrointestinal irritation is acted on, for common compatibe drug.
Chinese veterinarian's dialectical theory thinks that hydropsy for baby pigs is mainly since taste and small intestine are by damp and hot heresy or unclean trough
Infringement, the gas of impairing the spleen and stomach, make toxic dampness and heat product in intestines, and then develop be damp and hot tired spleen, temper it is big wound and cause empty water general,
Metabolism disorder, water retention, clinical manifestation go out various oedema signs.Therefore the cure mechanism of this prescription entirety: clearing heat and detoxicating, infiltration
Wet promoting the circulation of qi, inducing diuresis for removing edema, antimicrobial antiphlogistic, invigorating the spleen qi-regulating, heart tonifying.Prescription medicine complements each other, and has to hydropsy for baby pigs and is directed to
The treatment of property, the effect of reaching internal and external application, reinvigoration.China's Chinese Traditional Medicine apply when can not only treating both manifestation and root cause of disease, and
It is also adjustable body allomeric function, improves body autoimmune function, and prolonged application is safe and reliable.
Probiotics is inoculated with using bacterium solution, preferably lactic acid bacteria, and is more preferably the lactobacillus reuteri in genus lactubacillus
Bacillus is inoculated with by Chinese medicine composition and probiotics in 100:3 ratio, and the bacteria concentration of the probiotics bacterial liquid is >=108CFU/ml。
Fermentation temperature is 30~37 DEG C, and fermentation time is 18~36h.
The present invention also provides a kind of for preventing and treating the preparation method of the herbal mixture fermentation preparation of hydropsy for baby pigs, special
Sign is that steps are as follows:
(1) each traditional Chinese medicinal components are weighed according to quantity, are crushed, 40 mesh screens are crossed, and are uniformly mixed, it is spare；
(2) smashed Chinese medicine is added in fermentor, after impregnating 2h with 6 times of volume of water, by volume 1% MRS liquid is added
Culture medium is heated to 100 DEG C of holding 1.5h, is subsequently cooled to 30~37 DEG C；The lactic acid bacterial liquid of access pro rata, 30~37
DEG C fermentation 18~36h, filter to obtain primary filtrate, and filtrate is spare in 4 DEG C of refrigerations；
(3) dregs of a decoction taking-up is moved in extractor and is decocted 2 times again, be separately added into 6 times of volume of water and decoct 1h, filtration merges twice
The concentration of filtrate rotary evaporation low temperature is condensed into relative density not less than 1.3g/ml thick paste；
(4) gained thick paste is mixed evenly with primary filtrate, low temperature is concentrated to give the fermented tcm that concentration is about 1 g/ml and extracts
Liquid, i.e., every 1ml are equivalent to 1 g of crude drug, encapsulating, sterilizing, as herb fermenting preparation.
The traditional chinese medicine composition of the invention, each taste Chinese medicine are natural botanical source, and effective component is concentrated mainly on into the cell,
Using conventional method to traditional Chinese medicine extyaction after, the dregs of a decoction usually can be very much, and recovery rate is not also high, influence drug therapy effect
Fruit, and cause the waste of natural resources of Chinese medicinal materials.Documents and materials show, Radix Isatidis, Poria cocos, rde bean, jade in the traditional chinese medicine composition of the invention
A few taste medicinal materials such as rice palpus, Radix Astragali, dried orange peel, Radix Glycyrrhizae increase its active constituent content, to improve medicine after lactobacillus-fermented
Effect.Therefore the present invention utilizes lactobacillus-fermented the traditional chinese medicine composition of the invention, a series of enzymes generated in lactobacter growth can crack
Plant cell wall discharges intracellular reactive ingredient sufficiently, improves the content of effective component；Meanwhile it producing during the fermentation
Raw a large amount of lactic acid bacteria and its metabolite, can promote digestive system to keep fit, the nutritious supporting function of metabolite, benefit
In the premunition of enhancing animal body.
Specific embodiment
The invention will be further described with reference to embodiments, but the present invention is not limited only to these embodiments, is not taking off
Under the premise of from present inventive concept, made any change is all fallen within the scope of Protection Code of the invention.Affiliated strain is food
Grade strain.
Each group distribution ratio in 1 herbal mixture fermentation preparation of specific embodiment
Formula 1: 2.5 kg of radix scutellariae, 2.5kg parts of Radix Isatidis, 1.8 kg of Poria cocos, 1.8 kg of rde bean, 1.8 kg parts of corn stigma, Huang
1.5 kg parts of stilbene, 1.2 kg parts of ramulus cinnamomi, 1.2kg parts of dried orange peel, 0.7 kg of Radix Glycyrrhizae, lactobacillus reuteri 0.45kg.
Formula 2: 1.5 kg of radix scutellariae, 1.5kg parts of Radix Isatidis, Poria cocos 1.2kg, 1.2 kg of rde bean, 1.2 kg parts of corn stigma,
0.8 kg parts of Radix Astragali, 0.8 kg parts of ramulus cinnamomi, 0.8kg parts of dried orange peel, 0.5 kg of Radix Glycyrrhizae, lactobacillus reuteri 0.285kg.
It is formulated 3: 3.0 kg of radix scutellariae, 3.0kg parts of Radix Isatidis, 2.0 kg of Poria cocos, 2.0 kg of rde bean, 2.0 kg of corn stigma
Part, 1.8 kg parts of Radix Astragali, 1.8 kg parts of ramulus cinnamomi, 1.8kg parts of dried orange peel, 1.2 kg of Radix Glycyrrhizae, lactobacillus reuteri 0.56kg.
It is formulated 4: 2.8 kg of radix scutellariae, 2.4kg parts of Radix Isatidis, 1.8 kg of Poria cocos, 1.8 kg of rde bean, 1.6 kg of corn stigma
Part, 1.3 kg parts of Radix Astragali, 1.3 kg parts of ramulus cinnamomi, 1.3kg parts of dried orange peel, 0.7 kg of Radix Glycyrrhizae, lactobacillus reuteri 0.45kg.
The preparation method of 2 herbal mixture fermentation preparation of specific embodiment
By taking formula 1 as an example, the specific steps are as follows:
(1) traditional Chinese medicinal components are weighed according to quantity, are crushed, 40 mesh screens are crossed, and are uniformly mixed, it is spare；
(2) smashed Chinese medicine is added in fermentor, after impregnating 2h with 90L water, the MRS fluid nutrient medium of 9L is added, heated
To 100 DEG C of holding 1.5h, it is subsequently cooled to 35 DEG C；0.45kg lactic acid bacterial liquid is accessed, 37 DEG C of 36 h of fermentation filter to obtain initial filter
Liquid, and filtrate is spare in 4 DEG C of refrigerations；
(3) dregs of a decoction taking-up is moved in extractor and is decocted 2 times again, be separately added into 90L water and decoct 1h, filter,
Merge filtrate rotary evaporation low temperature twice to be concentrated, is condensed into relative density not less than 1.3g/ml thick paste；
(4) gained thick paste is mixed evenly with primary filtrate, low temperature is concentrated to give the fermented tcm that concentration is about 1 g/ml and extracts
Liquid, i.e., every 1ml are approximately equivalent to 1 g of crude drug, encapsulating, sterilizing, as herb fermenting preparation.
3 antibacterial experiment in vitro of specific embodiment
Escherichia coli used in experiment are to separate identification in hydropsy for baby pigs clinical case through this laboratory.Test choosing
Drug sensitive test is carried out with Odontothrips loti.
Bacterium solution preparation: 3 plants of Escherichia coli clinical separation strains that glycerol saves are inoculated in ordinary nutrient agar slant culture
Base, 37 DEG C constant temperature incubation 16~20 hours, lower lawn is washed with 2ml sterile saline in every inclined-plane, and adjusts bacterium with aqua sterilisa
Liquid concentration is 1.0 × 105Cfu/ml is spare.
It compares medical fluid: preparing Chinese medicine composition according to each traditional Chinese medicinal components ratio of formula 1, and specifically walked according to embodiment 2
Suddenly it extracts, ferments without using probiotics, No. 3 extracting solution rotary evaporation low temperature are concentrated into the Chinese medicine of about 1 g/ml
Extracting solution, i.e., every 1ml are approximately equivalent to crude drug 1g, and 105 DEG C sterilize 30 minutes, spare.
For reagent liquid: preparing Chinese medicine composition according to each traditional Chinese medicinal components ratio of formula 1, and specifically walked according to embodiment 2
Suddenly it extracts, is fermented using probiotics, herbal mixture fermentation preparation of the invention is made, i.e., every 1ml is approximately equivalent to give birth to
Medicine 1g.
Test method: in " super-clean bench ", Escherichia coli bacteria suspension even compact is coated on training with sterile cotton swabs
Primary surface is supported, and the Oxford cup of sterilizing is placed on by media surface with sterile working, light pressure makes it contact no sky with culture medium
Gap, each plate can put 3 Oxford cups, wherein 1 Oxford cup is put for reagent liquid, 1 puts control medical fluid, and 1 puts sterile physiological
Salt water negative control, accomplishes full without flowing over when adding medical fluid, and mark.It sets 37 DEG C to cultivate 12~18 hours, measures inhibition zone
Size simultaneously records.3 repetitions are arranged in every kind of bacterial strain.It is extremely quick, 15~20mm of antibacterial circle diameter that antibacterial circle diameter, which is greater than 20mm,
For Gao Min, antibacterial circle diameter be it is quick during 10~15mm is, it is muting sensitive that antibacterial circle diameter, which is less than 10mm, and no inhibition zone is not
It is sensitive
Test result, it is average by being acquired after measurement 3 repetition test group antibacterial circle diameter data of every plant of Escherichia coli separation strains
Value, see the table below 1, as the result is shown after lactobacillus-fermented compound Chinese medicinal preparation, compared with unfermentable compound Chinese medicinal preparation, to big
The bacteriostasis of enterobacteria significantly increases.
Bacteriostasis of the 1 herbal mixture fermentation preparation of table to Escherichia coli
4 acute toxicity test of specific embodiment
For reagent object: prepare Chinese medicine composition according to each traditional Chinese medicinal components ratio of formula 1, and according to 2 specific steps of embodiment into
Row extracts, and is fermented using lactic acid bacteria, and herbal mixture fermentation preparation of the present invention is made, i.e., every 1ml is approximately equivalent to crude drug 1g.
Test method:
Preliminary result shows that compound fermented traditional Chinese medicine formulations toxic of the present invention is very low, therefore using maximum dosage when test
Method, dosage are 5 ml/kg b.w..SD rat 40 are selected, half male and half female, after fasting 12h, administration group rat is according to 0.5 ml
Test solution is given in the stomach-filling of/100 g weight.Give after tested material diet after about 2 h of fasting.Control group is with identical
Method give pure water.Respectively observation is primary after continuous observation 30min, 2h, 4h after tested, and observation 1 time daily, continuous to see later
It examines 14 days, examines record animal subject diet, appearance, behavior, secretion, excreta abnormal conditions, observe and record dye
Malicious process and the daily poisoning manifestations of rat, death condition in the observation period, dissect rat cadavers in time, take disease when necessary
Dirty device carries out histopathological examination.(d0), the 1st d (d1), the 3rd d (d3), the 7th d (d7) on the day of administration
And 14d (d14) is weighed the weight of animals 1 time, and puts to death all survival rats of administration group after the observation period, is carried out
Pathological anatomy inspection.
When SD rat oral poisoning dosage is 5ml/kg b.w., all rats survive within the experimental observation phase, and essence
Refreshing movable normal, feeding, drinking-water, defecation etc. are normal, do not observe that toxic reaction, no rats death occurs in rat.It is all big
Mouse Normal-weight increases, and administration group rat body weight is in d0, d1, d3, d7, d14 there was no significant difference compared with the control group (P
> 0.05).Observation period terminates all survival rats of dissect, and Rats Organs and Tissues, which visually observe, has no that size, quality, color etc. occur bright
Show visible pathologic variation.The result shows that herbal mixture fermentation preparation rat oral contamination of the present invention, LD50Greater than 5 ml/
Kg prompts the dose safety of invention formulation clinical application higher.
The clinical test of the drug therapy hydropsy for baby pigs of the present invention of specific embodiment 5
In order to verify the present invention to the clinical therapeutic efficacy of hydropsy for baby pigs, we are with the present invention to pig natural infection edema disease of piglets
The case of disease carries out efficacy experiment.The present invention is divided into low, middle and high dose groups by test, with hydropsy for baby pigs infection control group, right
It is compared than medication therapy groups, to evaluate therapeutic effect and optimal treatment dosage of the invention.
1 test method
The clinical case of hydropsy for baby pigs is provided by Shenyang City, Liaoning Province Liaozhong County pig farm.By clinical observation,
Pathology dissect, and the separation for taking sick pig intestinal mucus pathological material of disease to carry out pathogen is identified, caused by being diagnosed as Escherichia coli
Hydropsy for baby pigs.The apparent piglet of experiment sieving clinical symptoms, i.e. eye, face obvious tumefaction, serious person's face, neck, head become
It is fat；Skin surface is general red, and rear-guard is powerless, waves and incoordination, individual sleeping ground do not rise, touching skin has paroxysmal to tremble；It flutters
When catching, cry is hoarse, and four limbs are in swimming shape after falling down to the ground.Sick pig is randomly divided into 5 groups, every group 15, each test group processing is as follows:
The test of table 2 grouping and administration
2 trial drugs
Chinese medicine composition is prepared according to each traditional Chinese medicinal components ratio of formula 1, and is extracted according to 2 specific steps of embodiment, is made
It is fermented with lactic acid bacteria, herbal mixture fermentation preparation of the present invention is made, i.e., every 1ml is approximately equivalent to crude drug 1g.
3 test results
The therapeutic effect of 3 hydropsy for baby pigs of table counts
Note: curative effect determinate standard:
Cure: the state of mind is normal, and feeding drinking-water restores normal, no clinical symptoms；
Improve: the state of mind is obviously improved, and feeding drinking-water increases, and still has fraction clinical symptoms not disappear；
Effective: the state of mind has improvement, feeding drinking behavior occurs, but there are also partial clinical symptoms；
Invalid: clinical symptoms are aggravated or are not obviously improved, and body temperature increases, palpebral edema, incidence enlargement,
Sporadic deaths.
4 interpretations of result
During test carries out, it is obviously improved 2-3 days test group sick pig symptoms are administered, appetite increases, especially
Test group 2 and 3,6-7 days most of sick pigs after administration restore normal.After test group 1 and the administration of drugs compared treatment group
Symptom has improvement within 4-5 days；Control group aggravation is infected, death increases.From the data of table 3 can be seen that test group with
Control group compares, and the total effective rate of test group 2,3 is apparently higher than test group 1 and drugs compared treatment group, illustrates Chinese medicine of the present invention
Fermentation preparation has effects that clearing heat and detoxicating, excreting dampness promoting the circulation of qi, inducing diuresis for removing edema, antimicrobial antiphlogistic, invigorating the spleen qi-regulating significantly, to piglet
It refreshes, appetite, improve disease resistance etc. with remarkable result.
To sum up, morbidity is directed to compared with anxious, the heavier sick pig of the state of an illness, and the clinical treatment dosage that the present invention recommends is 0.5ml/kg
Weight；It is directed to prevention hydropsy for baby pigs, the sick pig treatment of slight disease symptom can choose 0.25ml/kg weight.The present invention
Compound fermented traditional Chinese medicine preparation, composition is pure natural medical, containing plurality of active ingredients, and after probiotics fermention, effectively
Component content increases, and improves drug effect, and can enhance the premunition of animal body, does not generate drug resistance, highly-safe, noresidue and
Toxic side effect, and preparation process facilitates production, can be easy to promote the use of.
The foregoing is merely presently preferred embodiments of the present invention, can not be all at this to limit technical solution of the present invention
Within the spirit and scope of invention, any modification, equivalent replacement, improvement and so on should all cover and want in right of the invention
Within the scope of asking.
Claims (5)
1. a kind of for preventing and treating the Chinese medicine composition of hydropsy for baby pigs, which is characterized in that composed of the following components: radix scutellariae, plate are blue
Root, Poria cocos, rde bean, corn stigma, Radix Astragali, ramulus cinnamomi, dried orange peel, Radix Glycyrrhizae.
2. Chinese medicine composition according to claim 1, which is characterized in that each component mass fraction is respectively as follows: radix scutellariae
15~30 parts, 15~30 parts of Radix Isatidis, 12~20 parts of Poria cocos, 12~20 parts of rde bean, 12~20 parts of corn stigma, Radix Astragali 8
~18 parts, 8~18 parts of ramulus cinnamomi, 8~18 parts of dried orange peel, 5~12 parts of Radix Glycyrrhizae.
3. a kind of for preventing and treating the herbal mixture fermentation preparation of hydropsy for baby pigs, which is characterized in that by Chinese medicine composition with it is prebiotic
Bacterium is made in 100: 3 ratio mixed fermentations.
4. herbal mixture fermentation preparation according to claim 3, which is characterized in that the probiotics is saccharomycete, bifid
One or more of bacillus, lactic acid bacteria, bacillus subtilis.
5. a kind of for preventing and treating the preparation method of the herbal mixture fermentation preparation of hydropsy for baby pigs, which is characterized in that steps are as follows:
1) each traditional Chinese medicinal components are weighed according to quantity, are crushed, 40 mesh screens are crossed, and are uniformly mixed, it is spare；
2) smashed Chinese medicine is added in fermentor, after impregnating 2h with 6 times of volume of water, by volume 1% MRS liquid is added
Culture medium is heated to 100 DEG C of holding 1.5h, is subsequently cooled to 30~37 DEG C；The lactic acid bacterial liquid of access pro rata, 30~37
DEG C fermentation 18~36h, filter to obtain primary filtrate, and filtrate is spare in 4 DEG C of refrigerations；
3) dregs of a decoction taking-up is moved in extractor and is decocted 2 times again, be separately added into 6 times of volume of water and decoct 1h, filtration, merging is filtered twice
The concentration of liquid rotary evaporation low temperature is condensed into relative density not less than 1.3g/ml thick paste；
4) gained thick paste is mixed evenly with primary filtrate, low temperature is concentrated to give the fermented tcm that concentration is about 1 g/ml and extracts
Liquid, i.e., every 1ml are equivalent to 1 g of crude drug, encapsulating, sterilizing, as herb fermenting preparation.
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