CN109997875A - A kind of microbial bacterial agent and preparation method thereof with pest-resistant sterilizing function - Google Patents
A kind of microbial bacterial agent and preparation method thereof with pest-resistant sterilizing function Download PDFInfo
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- CN109997875A CN109997875A CN201910325919.0A CN201910325919A CN109997875A CN 109997875 A CN109997875 A CN 109997875A CN 201910325919 A CN201910325919 A CN 201910325919A CN 109997875 A CN109997875 A CN 109997875A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention discloses a kind of microbial bacterial agent with pest-resistant sterilizing function, belongs to microorganisms technical field.Microbial inoculum of the present invention is prepared by the raw material of following parts by weight: 10-20 parts of bafillus natto, 10-20 parts of bacillus subtilis, 10-20 parts of Dipel, 3-5 parts of rice Pseudomonas alba of dwelling, 5-15 parts of azotobacter chroococcum, 1-3 parts of dispersing agent, 1-3 parts of reinforcing agent, 50-80 parts of decentralized medium.Synergistic effect, Relationship With Proliferation form the biological flora that complexity, stable structure, a function widely have varied bacterial micro-organism group each other between each component of the present invention, pest-resistant fungistatic effect is obvious, simple and easy, cost is not high, has a vast market foreground.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of microbial bacterial agent with pest-resistant sterilizing function and its
Preparation method.
Background technique
Chemical pesticide improves grain yield, it is raw to meet the mankind in control phytopathy, worm, crop smothering, coordinate plant growth development
It deposits in needs and has played positive effect, made huge contribution.For a long time, pesticide residue and persistent problem are to farming
Object influence is very big, especially for vegetable melon and fruit, the pesticide of the various pesticide-germicides of multiple applications is often needed in growth period, directly
Connect the health for endangering the people.
Microbial bacterial agent has the advantages that efficient, safe and environment-friendly as a kind of new type disinsection microbial inoculum.But general microorganism
Microbial inoculum can only desinsection or sterilization, both can not accomplish to take into account, and mushroom insecticide exists and knocks down pest overlong time and anti-
The disadvantages of effect is unstable is controlled, therefore how to improve the efficiency of these biological insecticides, and makes it have the anti-of long stable effect
Cure rate is the key point of such microbial inoculum.Secondly, agriculture application aspect development of the microbial bacterial agent in China is slower, due to
Scientific research strength is weak, does not break through in the technical aspects such as bacteria selection, domestication and fermentation technique, strain compatibility；It is applying
Aspect and scientific research even more disconnect, shortage accurately applies data, make popularization and application job development lag, much related in the market to produce
National standard is not achieved in product, and effect is unobvious, is less useful for it and popularizes.
Summary of the invention
Good object of the present invention is to overcome the deficiencies of the prior art and provide a kind of pesticide-germicide effect, universality is strong, and can be with
The microbial bacterial agent of soil environment is adjusted, and discloses its specific preparation method.The technical scheme adopted by the invention is that:
A kind of microbial bacterial agent with pest-resistant sterilizing function is to be prepared by the raw material of following parts by weight: natto bud
10-20 parts of spore bacillus, 10-20 parts of bacillus subtilis, 10-20 parts of Dipel, 3-5 parts of rice Pseudomonas alba of dwelling, circle are brown
5-15 parts of nitrogen-fixing bacteria, 1-3 parts of dispersing agent, 1-3 parts of reinforcing agent, 50-80 parts of decentralized medium.
The reinforcing agent is porous magnetic particle, is specifically prepared by following methods: 20 ferrous sulfate and 20g chlorine
Change iron to be dissolved in 500mL deionized water, 18g Powdered Activated Carbon is added, magnetic agitation 30min, heated solution is to 70 DEG C；Under stirring
5mol/L sodium hydroxide solution 100mL is added dropwise, mixture is then aged 4h in 100 DEG C of water-baths；Be cooled to room temperature, spend from
Sub- water washed product repeatedly；Dry 4h at 102 DEG C -105 DEG C, obtains the porous magnetic particle in baking oven.
The dispersing agent is that Sodium Polyacrylate and sodium lignosulfonate are mixed to get according to mass ratio 3:1.
The decentralized medium is diatomite or attapulgite.
A kind of preparation method of the microbial bacterial agent with pest-resistant sterilizing function, comprising the following steps:
(1) each two ring of dwell rice Pseudomonas alba, azotobacter chroococcum for taking inclined-plane conservation respectively, is inoculated in beef extract egg respectively
White peptone culture medium, 37 DEG C of cultivation temperature, incubation time 20h, the seed liquor of must dwell respectively rice Pseudomonas alba, azotobacter chroococcum；
By seed liquor by 5% inoculum concentration access 500L seed tank culture base, 35 DEG C, cultivate for 24 hours under the conditions of 180r/min, must dwell respectively
Rice Pseudomonas alba, azotobacter chroococcum bacterium solution；
(2) rice Pseudomonas alba, the azotobacter chroococcum bacterium solution of dwelling are adsorbed using decentralized medium, plate and frame filter press after the completion of absorption
Filters pressing, spray drying, 140 DEG C of inlet air temperature, feed rate 17ml/min respectively obtain rice Pseudomonas alba of dwelling, the brown fixed nitrogen of circle
Bacteria agent；
(3) prepared by reinforcing agent: 20 ferrous sulfate and 20g iron chloride being dissolved in 500mL deionized water, 18g powder is added
Active carbon, magnetic agitation 30min, heated solution is to 70 DEG C；Stirring is lower to be added dropwise 5mol/L sodium hydroxide solution 100mL, then
Mixture is aged 4h in 100 DEG C of water-baths；It is cooled to room temperature, with deionized water washed product repeatedly；102 DEG C -105 DEG C in baking oven
Lower dry 4h, obtains the reinforcing agent；
(4) according to weight ratio by bafillus natto, bacillus subtilis, Dipel, dwell rice Pseudomonas alba bacterium
Agent, azotobacter chroococcum microbial inoculum are uniformly mixed with dispersing agent, reinforcing agent, are added to airslide disintegrating mill, and fineness is controlled in 500-800
Mesh obtains the microbial bacterial agent after dry.
Described, the concrete composition of beef-protein medium: beef extract 5g/L, peptone 10g/L, NaCl5g/L,
Agar 15g/L, remaining is water.
Described, the concrete composition of seed tank culture base: beef extract 0.3%, peptone 1%, sodium chloride 0.5%, grape
Sugar 0.5%.
Bafillus natto of the invention, bacillus subtilis, Dipel are commercially available, living bacteria count 100-
20000000000/gram.
Rice Pseudomonas alba used in the present invention of dwelling, azotobacter chroococcum are purchased from China General Microbiological culture presevation management
Center (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica), deposit number is respectively as follows:
CGMCC No.15325、CGMCC No.12402。
Beneficial effects of the present invention:
(1) present invention uses bacillus subtilis, can produce the active material of subtilin, gramicidins etc., to cause
Germ has apparent inhibiting effect, while it can induce crop and generates the substances such as heteroauxin, promote as growth；And Su Yunjin
Bacillus is a kind of efficient biological insecticides, and the endotoxin and exotoxin which generates can make pest stop feed, finally cause
Pest is dead；
(2) the rice Pseudomonas alba of dwelling that adds of the present invention, phenolic acid class noxious material in the soil that can effectively degrade；
(3) effective microbial inoculum is adsorbed in dispersible carrier by the present invention, realizes the efficient release of microbial inoculum, while adding porous
Magnetic-particle is reinforcing agent, and one side can provide certain ferro element, high-specific surface area and high absorption for crop soil
Property, effective bacterium can also be adsorbed, the biological bacteria and reinforcing agent of addition cooperate, common efficient performance inventive formulation
Pest-resistant sterilizing ability；
One synergistic effect, Relationship With Proliferation formation complexity, stable structure, function are extensive each other between each component of the present invention
Biological flora with varied bacterial micro-organism group, pest-resistant fungistatic effect is obvious, and simple and easy, cost is not high, has
Vast market prospect.
Specific embodiment
Technical scheme is described further combined with specific embodiments below, but not limited to this.
Embodiment 1
A kind of microbial bacterial agent with pest-resistant sterilizing function is to be prepared by the raw material of following parts by weight: natto bud
10 parts of spore bacillus, 10 parts of bacillus subtilis, 10 parts of Dipel, 3 parts of rice Pseudomonas alba of dwelling, azotobacter chroococcum 5 part, point
1 part of powder, 1 part of reinforcing agent, 50 parts of decentralized medium.
The reinforcing agent is porous magnetic particle, is specifically prepared by following methods: 20 ferrous sulfate and 20g chlorine
Change iron to be dissolved in 500mL deionized water, 18g Powdered Activated Carbon is added, magnetic agitation 30min, heated solution is to 70 DEG C；Under stirring
5mol/L sodium hydroxide solution 100mL is added dropwise, mixture is then aged 4h in 100 DEG C of water-baths；Be cooled to room temperature, spend from
Sub- water washed product repeatedly；Dry 4h at 102 DEG C, obtains the porous magnetic particle in baking oven.
The dispersing agent is that Sodium Polyacrylate and sodium lignosulfonate are mixed to get according to mass ratio 3:1.
The decentralized medium is diatomite.
A kind of preparation method of the microbial bacterial agent with pest-resistant sterilizing function, comprising the following steps:
(1) each two ring of dwell rice Pseudomonas alba, azotobacter chroococcum for taking inclined-plane conservation respectively, is inoculated in beef extract egg respectively
White peptone culture medium, 37 DEG C of cultivation temperature, incubation time 20h, the seed liquor of must dwell respectively rice Pseudomonas alba, azotobacter chroococcum；
By seed liquor by 5% inoculum concentration access 500L seed tank culture base, 35 DEG C, cultivate for 24 hours under the conditions of 180r/min, must dwell respectively
Rice Pseudomonas alba, azotobacter chroococcum bacterium solution；
(2) rice Pseudomonas alba, the azotobacter chroococcum bacterium solution of dwelling are adsorbed using decentralized medium, plate and frame filter press after the completion of absorption
Filters pressing, spray drying, 140 DEG C of inlet air temperature, feed rate 17ml/min respectively obtain rice Pseudomonas alba of dwelling, the brown fixed nitrogen of circle
Bacteria agent；
(3) prepared by reinforcing agent: 20 ferrous sulfate and 20g iron chloride being dissolved in 500mL deionized water, 18g powder is added
Active carbon, magnetic agitation 30min, heated solution is to 70 DEG C；Stirring is lower to be added dropwise 5mol/L sodium hydroxide solution 100mL, then
Mixture is aged 4h in 100 DEG C of water-baths；It is cooled to room temperature, with deionized water washed product repeatedly；It is dry at 102 DEG C in baking oven
4h obtains the reinforcing agent；
(4) according to weight ratio by bafillus natto, bacillus subtilis, Dipel, dwell rice Pseudomonas alba bacterium
Agent, azotobacter chroococcum microbial inoculum are uniformly mixed with dispersing agent, reinforcing agent, are added to airslide disintegrating mill, and fineness is controlled in 500-800
Mesh obtains the microbial bacterial agent after dry.
Described, the concrete composition of beef-protein medium: beef extract 5g/L, peptone 10g/L, NaCl5g/L,
Agar 15g/L, remaining is water.
Described, the concrete composition of seed tank culture base: beef extract 0.3%, peptone 1%, sodium chloride 0.5%, grape
Sugar 0.5%.
Bafillus natto of the invention, bacillus subtilis, Dipel are commercially available, living bacteria count 100-
20000000000/gram.
Rice Pseudomonas alba used in the present invention of dwelling, azotobacter chroococcum are purchased from China General Microbiological culture presevation management
Center (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica), deposit number is respectively as follows:
CGMCC No.15325、CGMCC No.12402。
Embodiment 2
A kind of microbial bacterial agent with pest-resistant sterilizing function is to be prepared by the raw material of following parts by weight: natto bud
15 parts of spore bacillus, 15 parts of bacillus subtilis, 15 parts of Dipel, 4 parts of rice Pseudomonas alba of dwelling, 10 parts of azotobacter chroococcum,
2 parts of dispersing agent, 2 parts of reinforcing agent, 65 parts of decentralized medium.
The reinforcing agent is porous magnetic particle, is specifically prepared by following methods: 20 ferrous sulfate and 20g chlorine
Change iron to be dissolved in 500mL deionized water, 18g Powdered Activated Carbon is added, magnetic agitation 30min, heated solution is to 70 DEG C；Under stirring
5mol/L sodium hydroxide solution 100mL is added dropwise, mixture is then aged 4h in 100 DEG C of water-baths；Be cooled to room temperature, spend from
Sub- water washed product repeatedly；Dry 4h at 103 DEG C, obtains the porous magnetic particle in baking oven.
The dispersing agent is that Sodium Polyacrylate and sodium lignosulfonate are mixed to get according to mass ratio 3:1.
The decentralized medium is attapulgite.
A kind of preparation method of the microbial bacterial agent with pest-resistant sterilizing function, comprising the following steps:
(1) each two ring of dwell rice Pseudomonas alba, azotobacter chroococcum for taking inclined-plane conservation respectively, is inoculated in beef extract egg respectively
White peptone culture medium, 37 DEG C of cultivation temperature, incubation time 20h, the seed liquor of must dwell respectively rice Pseudomonas alba, azotobacter chroococcum；
By seed liquor by 5% inoculum concentration access 500L seed tank culture base, 35 DEG C, cultivate for 24 hours under the conditions of 180r/min, must dwell respectively
Rice Pseudomonas alba, azotobacter chroococcum bacterium solution；
(2) rice Pseudomonas alba, the azotobacter chroococcum bacterium solution of dwelling are adsorbed using decentralized medium, plate and frame filter press after the completion of absorption
Filters pressing, spray drying, 140 DEG C of inlet air temperature, feed rate 17ml/min respectively obtain rice Pseudomonas alba of dwelling, the brown fixed nitrogen of circle
Bacteria agent；
(3) prepared by reinforcing agent: 20 ferrous sulfate and 20g iron chloride being dissolved in 500mL deionized water, 18g powder is added
Active carbon, magnetic agitation 30min, heated solution is to 70 DEG C；Stirring is lower to be added dropwise 5mol/L sodium hydroxide solution 100mL, then
Mixture is aged 4h in 100 DEG C of water-baths；It is cooled to room temperature, with deionized water washed product repeatedly；It is dry at 103 DEG C in baking oven
4h obtains the reinforcing agent；
(4) according to weight ratio by bafillus natto, bacillus subtilis, Dipel, dwell rice Pseudomonas alba bacterium
Agent, azotobacter chroococcum microbial inoculum are uniformly mixed with dispersing agent, reinforcing agent, are added to airslide disintegrating mill, and fineness is controlled in 500-800
Mesh obtains the microbial bacterial agent after dry.
Described, the concrete composition of beef-protein medium: beef extract 5g/L, peptone 10g/L, NaCl5g/L,
Agar 15g/L, remaining is water.
Described, the concrete composition of seed tank culture base: beef extract 0.3%, peptone 1%, sodium chloride 0.5%, grape
Sugar 0.5%.
Bafillus natto of the invention, bacillus subtilis, Dipel are commercially available, living bacteria count 100-
20000000000/gram.
Rice Pseudomonas alba used in the present invention of dwelling, azotobacter chroococcum are purchased from China General Microbiological culture presevation management
Center (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica), deposit number is respectively as follows:
CGMCC No.15325、CGMCC No.12402。
Embodiment 3
A kind of microbial bacterial agent with pest-resistant sterilizing function is to be prepared by the raw material of following parts by weight: natto bud
20 parts of spore bacillus, 20 parts of bacillus subtilis, 20 parts of Dipel, 5 parts of rice Pseudomonas alba of dwelling, 15 parts of azotobacter chroococcum,
3 parts of dispersing agent, 3 parts of reinforcing agent, 80 parts of decentralized medium.
The reinforcing agent is porous magnetic particle, is specifically prepared by following methods: 20 ferrous sulfate and 20g chlorine
Change iron to be dissolved in 500mL deionized water, 18g Powdered Activated Carbon is added, magnetic agitation 30min, heated solution is to 70 DEG C；Under stirring
5mol/L sodium hydroxide solution 100mL is added dropwise, mixture is then aged 4h in 100 DEG C of water-baths；Be cooled to room temperature, spend from
Sub- water washed product repeatedly；Dry 4h at 105 DEG C, obtains the porous magnetic particle in baking oven.
The dispersing agent is that Sodium Polyacrylate and sodium lignosulfonate are mixed to get according to mass ratio 3:1.
The decentralized medium is diatomite.
A kind of preparation method of the microbial bacterial agent with pest-resistant sterilizing function, comprising the following steps:
(1) each two ring of dwell rice Pseudomonas alba, azotobacter chroococcum for taking inclined-plane conservation respectively, is inoculated in beef extract egg respectively
White peptone culture medium, 37 DEG C of cultivation temperature, incubation time 20h, the seed liquor of must dwell respectively rice Pseudomonas alba, azotobacter chroococcum；
By seed liquor by 5% inoculum concentration access 500L seed tank culture base, 35 DEG C, cultivate for 24 hours under the conditions of 180r/min, must dwell respectively
Rice Pseudomonas alba, azotobacter chroococcum bacterium solution；
(2) rice Pseudomonas alba, the azotobacter chroococcum bacterium solution of dwelling are adsorbed using decentralized medium, plate and frame filter press after the completion of absorption
Filters pressing, spray drying, 140 DEG C of inlet air temperature, feed rate 17ml/min respectively obtain rice Pseudomonas alba of dwelling, the brown fixed nitrogen of circle
Bacteria agent；
(3) prepared by reinforcing agent: 20 ferrous sulfate and 20g iron chloride being dissolved in 500mL deionized water, 18g powder is added
Active carbon, magnetic agitation 30min, heated solution is to 70 DEG C；Stirring is lower to be added dropwise 5mol/L sodium hydroxide solution 100mL, then
Mixture is aged 4h in 100 DEG C of water-baths；It is cooled to room temperature, with deionized water washed product repeatedly；It is dry at 105 DEG C in baking oven
4h obtains the reinforcing agent；
(4) according to weight ratio by bafillus natto, bacillus subtilis, Dipel, dwell rice Pseudomonas alba bacterium
Agent, azotobacter chroococcum microbial inoculum are uniformly mixed with dispersing agent, reinforcing agent, are added to airslide disintegrating mill, and fineness is controlled in 500-800
Mesh obtains the microbial bacterial agent after dry.
Described, the concrete composition of beef-protein medium: beef extract 5g/L, peptone 10g/L, NaCl5g/L,
Agar 15g/L, remaining is water.
Described, the concrete composition of seed tank culture base: beef extract 0.3%, peptone 1%, sodium chloride 0.5%, grape
Sugar 0.5%.
Bafillus natto of the invention, bacillus subtilis, Dipel are commercially available, living bacteria count 100-
20000000000/gram.
Rice Pseudomonas alba used in the present invention of dwelling, azotobacter chroococcum are purchased from China General Microbiological culture presevation management
Center (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica), deposit number is respectively as follows:
CGMCC No.15325、CGMCC No.12402。
Comparative example 1
A kind of microbial bacterial agent with pest-resistant sterilizing function is to be prepared by the raw material of following parts by weight: natto bud
20 parts of spore bacillus, 20 parts of bacillus subtilis, 20 parts of Dipel, 5 parts of rice Pseudomonas alba of dwelling, 15 parts of azotobacter chroococcum,
3 parts of dispersing agent, 80 parts of decentralized medium.
The dispersing agent is that Sodium Polyacrylate and sodium lignosulfonate are mixed to get according to mass ratio 3:1.
The decentralized medium is diatomite.
A kind of preparation method of the microbial bacterial agent with pest-resistant sterilizing function, comprising the following steps:
(1) each two ring of dwell rice Pseudomonas alba, azotobacter chroococcum for taking inclined-plane conservation respectively, is inoculated in beef extract egg respectively
White peptone culture medium, 37 DEG C of cultivation temperature, incubation time 20h, the seed liquor of must dwell respectively rice Pseudomonas alba, azotobacter chroococcum；
By seed liquor by 5% inoculum concentration access 500L seed tank culture base, 35 DEG C, cultivate for 24 hours under the conditions of 180r/min, must dwell respectively
Rice Pseudomonas alba, azotobacter chroococcum bacterium solution；
(2) rice Pseudomonas alba, the azotobacter chroococcum bacterium solution of dwelling are adsorbed using decentralized medium, plate and frame filter press after the completion of absorption
Filters pressing, spray drying, 140 DEG C of inlet air temperature, feed rate 17ml/min respectively obtain rice Pseudomonas alba of dwelling, the brown fixed nitrogen of circle
Bacteria agent；
(3) according to weight ratio by bafillus natto, bacillus subtilis, Dipel, dwell rice Pseudomonas alba bacterium
Agent, azotobacter chroococcum microbial inoculum are uniformly mixed with dispersing agent, reinforcing agent, are added to airslide disintegrating mill, and fineness is controlled in 500-800
Mesh obtains the microbial bacterial agent after dry.
Described, the concrete composition of beef-protein medium: beef extract 5g/L, peptone 10g/L, NaCl5g/L,
Agar 15g/L, remaining is water.
Described, the concrete composition of seed tank culture base: beef extract 0.3%, peptone 1%, sodium chloride 0.5%, grape
Sugar 0.5%.
Bafillus natto of the invention, bacillus subtilis, Dipel are commercially available, living bacteria count 100-
20000000000/gram.
Rice Pseudomonas alba used in the present invention of dwelling, azotobacter chroococcum are purchased from China General Microbiological culture presevation management
Center (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica), deposit number is respectively as follows:
CGMCC No.15325、CGMCC No.12402。
Comparative example 2
A kind of microbial bacterial agent with pest-resistant sterilizing function is to be prepared by the raw material of following parts by weight: natto bud
20 parts of spore bacillus, 20 parts of bacillus subtilis, 20 parts of Dipel, 15 parts of azotobacter chroococcum, 3 parts of dispersing agent, reinforcing agent 3
Part, 80 parts of decentralized medium.
The reinforcing agent is porous magnetic particle, is specifically prepared by following methods: 20 ferrous sulfate and 20g chlorine
Change iron to be dissolved in 500mL deionized water, 18g Powdered Activated Carbon is added, magnetic agitation 30min, heated solution is to 70 DEG C；Under stirring
5mol/L sodium hydroxide solution 100mL is added dropwise, mixture is then aged 4h in 100 DEG C of water-baths；Be cooled to room temperature, spend from
Sub- water washed product repeatedly；Dry 4h at 105 DEG C, obtains the porous magnetic particle in baking oven.
The dispersing agent is that Sodium Polyacrylate and sodium lignosulfonate are mixed to get according to mass ratio 3:1.
The decentralized medium is diatomite.
A kind of preparation method of the microbial bacterial agent with pest-resistant sterilizing function, comprising the following steps:
(1) two ring of azotobacter chroococcum for taking inclined-plane conservation respectively is inoculated in beef-protein medium, culture temperature respectively
37 DEG C of degree, incubation time 20h obtains the seed liquor of azotobacter chroococcum；Seed liquor is accessed into 500L seed tank culture by 5% inoculum concentration
In base, 35 DEG C, cultivate for 24 hours under the conditions of 180r/min, azotobacter chroococcum bacterium solution is obtained；
(2) azotobacter chroococcum bacterium solution is adsorbed using decentralized medium, plate and frame filter press filters pressing after the completion of absorption, spray drying,
140 DEG C of inlet air temperature, feed rate 17ml/min, obtain azotobacter chroococcum microbial inoculum；
(3) prepared by reinforcing agent: 20 ferrous sulfate and 20g iron chloride being dissolved in 500mL deionized water, 18g powder is added
Active carbon, magnetic agitation 30min, heated solution is to 70 DEG C；Stirring is lower to be added dropwise 5mol/L sodium hydroxide solution 100mL, then
Mixture is aged 4h in 100 DEG C of water-baths；It is cooled to room temperature, with deionized water washed product repeatedly；It is dry at 105 DEG C in baking oven
4h obtains the reinforcing agent；
(4) according to weight ratio by bafillus natto, bacillus subtilis, Dipel, azotobacter chroococcum microbial inoculum with
Dispersing agent, reinforcing agent are uniformly mixed, and are added to airslide disintegrating mill, and fineness control obtains micro- life after dry in 500-800 mesh
Object microbial inoculum.
Described, the concrete composition of beef-protein medium: beef extract 5g/L, peptone 10g/L, NaCl5g/L,
Agar 15g/L, remaining is water.
Described, the concrete composition of seed tank culture base: beef extract 0.3%, peptone 1%, sodium chloride 0.5%, grape
Sugar 0.5%.
Bafillus natto of the invention, bacillus subtilis, Dipel are commercially available, living bacteria count 100-
20000000000/gram.
Azotobacter chroococcum used in the present invention is purchased from China General Microbiological culture presevation administrative center (address: Beijing
No. 3 Institute of Microorganism, Academia Sinica, institute of city, North Star West Road, Chaoyang District 1), deposit number are as follows: CGMCC No.12402.
Comparative example 3
A kind of microbial bacterial agent with pest-resistant sterilizing function is to be prepared by the raw material of following parts by weight: natto bud
20 parts of spore bacillus, 20 parts of bacillus subtilis, 20 parts of Dipel, 15 parts of azotobacter chroococcum, 3 parts of dispersing agent, decentralized medium
80 parts.
The dispersing agent is that Sodium Polyacrylate and sodium lignosulfonate are mixed to get according to mass ratio 3:1.
The decentralized medium is diatomite.
A kind of preparation method of the microbial bacterial agent with pest-resistant sterilizing function, comprising the following steps:
(1) two ring of azotobacter chroococcum for taking inclined-plane conservation respectively is inoculated in beef-protein medium, culture temperature respectively
37 DEG C of degree, incubation time 20h obtains the seed liquor of azotobacter chroococcum；Seed liquor is accessed into 500L seed tank culture by 5% inoculum concentration
In base, 35 DEG C, cultivate for 24 hours under the conditions of 180r/min, azotobacter chroococcum bacterium solution is obtained；
(2) azotobacter chroococcum bacterium solution is adsorbed using decentralized medium, plate and frame filter press filters pressing after the completion of absorption, spray drying,
140 DEG C of inlet air temperature, feed rate 17ml/min, obtain azotobacter chroococcum microbial inoculum；
(3) according to weight ratio by bafillus natto, bacillus subtilis, Dipel, azotobacter chroococcum microbial inoculum with
Dispersing agent, reinforcing agent are uniformly mixed, and are added to airslide disintegrating mill, and fineness control obtains micro- life after dry in 500-800 mesh
Object microbial inoculum.
Described, the concrete composition of beef-protein medium: beef extract 5g/L, peptone 10g/L, NaCl5g/L,
Agar 15g/L, remaining is water.
Described, the concrete composition of seed tank culture base: beef extract 0.3%, peptone 1%, sodium chloride 0.5%, grape
Sugar 0.5%.
Bafillus natto of the invention, bacillus subtilis, Dipel are commercially available, living bacteria count 100-
20000000000/gram.
Azotobacter chroococcum used in the present invention is purchased from China General Microbiological culture presevation administrative center (address: Beijing
No. 3 Institute of Microorganism, Academia Sinica, institute of city, North Star West Road, Chaoyang District 1), deposit number are as follows: CGMCC No.12402.
Product quality inspection
Product is carried out according to (GB20287-2006) " agricultural microbial agent " method, to microbial inoculum obtained by embodiment 1-3 into
Row measurement, measurement result such as table 1:
1 testing result of table
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Living bacteria count | 4.0 hundred million/gram | 4.2 hundred million/gram | 4.3 hundred million/gram |
| Moisture | 5.2 | 6.3 | 6.0 |
| Appearance | Particle is uniform | Particle is uniform | Particle is uniform |
| Miscellaneous bacteria rate | 0.9% | 1.0% | 1.1% |
Experimental design
For trial product: microbial bacterial agent obtained by 1-3 of the embodiment of the present invention, microbial inoculum obtained by comparative example 1-3 of the present invention, blank pair
Ratio；
For trying vegetables: tomato, cucumber, Chinese cabbage, eggplant, capsicum
Application method: point 7 processing groups, S1-S3 application 1-3 of embodiment of the present invention gained microbial bacterial agent, 6 kgs/acre；
Microbial bacterial agent obtained by S4-S6 application comparative example 1-3,6 kgs/acre；
S7 does not apply any microbial inoculum；
Table 2 tests vegetables insect pest bacteria-treating rate (%)
| Tomato | Cucumber | Chinese cabbage | Eggplant | Capsicum | |
| Embodiment 1 | 90.2 | 92.5 | 89.3 | 85.9 | 92.6 |
| Embodiment 2 | 91.5 | 93.2 | 90.2 | 86.5 | 95.3 |
| Embodiment 3 | 93.3 | 94.8 | 91.5 | 89.9 | 95.6 |
| Comparative example 1 | 50.3 | 40.6 | 36.8 | 40.3 | 32.1 |
| Comparative example 2 | 45.6 | 36.2 | 35.2 | 40.0 | 30.6 |
| Comparative example 3 | 30.6 | 32.6 | 34.1 | 35.2 | 29.6 |
| Blank example | 8.2 | 10.2 | 11.3 | 10.1 | 9.8 |
It should be noted that above-described embodiment is only the section Example for realizing preferred embodiment of the invention, Er Feiquan
Portion's embodiment.Obviously, it is based on the above embodiment of the present invention, those of ordinary skill in the art are not making creative work
Under the premise of other all embodiments obtained, should fall within the scope of the present invention.
Claims (5)
1. a kind of microbial bacterial agent with pest-resistant sterilizing function, which is characterized in that be by following parts by weight raw material preparation and
At: 10-20 parts of bafillus natto, 10-20 parts of bacillus subtilis, 10-20 parts of Dipel, rice Pseudomonas alba 3- of dwelling
5 parts, 5-15 parts of azotobacter chroococcum, 1-3 parts of dispersing agent, 1-3 parts of reinforcing agent, 50-80 parts of decentralized medium.
2. according to claim 1 with the microbial bacterial agent of pest-resistant sterilizing function, which is characterized in that the reinforcing agent is more
Hole magnetic-particle, is specifically prepared by following methods: 20 ferrous sulfate and 20g iron chloride are dissolved in 500mL deionized water
In, 18g Powdered Activated Carbon is added, magnetic agitation 30min, heated solution is to 70 DEG C；The lower dropwise addition 5mol/L sodium hydroxide of stirring is molten
Then mixture is aged 4h in 100 DEG C of water-baths by liquid 100mL；It is cooled to room temperature, with deionized water washed product repeatedly；It dries
Dry 4h at 102 DEG C -105 DEG C, obtains the porous magnetic particle in case.
3. according to claim 1 with the microbial bacterial agent of pest-resistant sterilizing function, which is characterized in that the dispersing agent is poly-
Sodium acrylate and sodium lignosulfonate are mixed to get according to mass ratio 3:1.
4. according to claim 1 with the microbial bacterial agent of pest-resistant sterilizing function, which is characterized in that the decentralized medium is
Diatomite or attapulgite.
5. a kind of preparation method of the microbial bacterial agent described in claim 1-4 any one with pest-resistant sterilizing function, special
Sign is, comprising the following steps:
(1) each two ring of dwell rice Pseudomonas alba, azotobacter chroococcum for taking inclined-plane conservation respectively, is inoculated in beef extract-peptone respectively
Culture medium, 37 DEG C of cultivation temperature, incubation time 20h, the seed liquor of must dwell respectively rice Pseudomonas alba, azotobacter chroococcum；It will kind
Sub- liquid is by 5% inoculum concentration access 500L seed tank culture base, and 35 DEG C, cultivate for 24 hours under the conditions of 180r/min, the rice that must dwell respectively is false
Pseudomonas bacillus, azotobacter chroococcum bacterium solution；
(2) rice Pseudomonas alba, the azotobacter chroococcum bacterium solution of dwelling are adsorbed using decentralized medium, plate and frame filter press pressure after the completion of absorption
Filter, spray drying, 140 DEG C of inlet air temperature, feed rate 17ml/min respectively obtain dwell rice Pseudomonas alba, azotobacter chroococcum
Microbial inoculum；
(3) prepared by reinforcing agent: 20 ferrous sulfate and 20g iron chloride being dissolved in 500mL deionized water, 18g powder activity is added
Charcoal, magnetic agitation 30min, heated solution is to 70 DEG C；Stirring is lower to be added dropwise 5mol/L sodium hydroxide solution 100mL, then mixing
Object is aged 4h in 100 DEG C of water-baths；It is cooled to room temperature, with deionized water washed product repeatedly；It is done at 102 DEG C -105 DEG C in baking oven
Dry 4h obtains the reinforcing agent；
(4) according to weight ratio by bafillus natto, bacillus subtilis, Dipel, rice Pseudomonas alba microbial inoculum of dwelling,
Azotobacter chroococcum microbial inoculum is uniformly mixed with dispersing agent, reinforcing agent, is added to airslide disintegrating mill, and fineness control is done in 500-800 mesh
The microbial bacterial agent is obtained after dry.
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| CN108485990A (en) * | 2018-04-03 | 2018-09-04 | 山东丰乐生物科技有限公司 | One kind having prevention and control apple tree root rot microbial bacterial agent and preparation method thereof |
| CN108546655A (en) * | 2018-04-03 | 2018-09-18 | 沈阳农业大学 | A kind of melon crop rhizosphere suppression harmful bacteria degradation bacteria and its application |
| CN108675886A (en) * | 2018-08-10 | 2018-10-19 | 湖北茂盛生物有限公司 | A kind of selenium-rich zoology slow release fertilizer and preparation method thereof |
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| CN108485990A (en) * | 2018-04-03 | 2018-09-04 | 山东丰乐生物科技有限公司 | One kind having prevention and control apple tree root rot microbial bacterial agent and preparation method thereof |
| CN108546655A (en) * | 2018-04-03 | 2018-09-18 | 沈阳农业大学 | A kind of melon crop rhizosphere suppression harmful bacteria degradation bacteria and its application |
| CN108675886A (en) * | 2018-08-10 | 2018-10-19 | 湖北茂盛生物有限公司 | A kind of selenium-rich zoology slow release fertilizer and preparation method thereof |
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