CN109997794A - Sulphadiazine is to the application in the T. pretiosum reproductive behavio(u)r of infection Wolbachia - Google Patents
Sulphadiazine is to the application in the T. pretiosum reproductive behavio(u)r of infection Wolbachia Download PDFInfo
- Publication number
- CN109997794A CN109997794A CN201910301264.3A CN201910301264A CN109997794A CN 109997794 A CN109997794 A CN 109997794A CN 201910301264 A CN201910301264 A CN 201910301264A CN 109997794 A CN109997794 A CN 109997794A
- Authority
- CN
- China
- Prior art keywords
- wolbachia
- sulphadiazine
- pretiosum
- application
- animal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000604961 Wolbachia Species 0.000 title claims abstract description 63
- 241000256619 Trichogramma pretiosum Species 0.000 title claims abstract description 39
- SEEPANYCNGTZFQ-UHFFFAOYSA-N sulfadiazine Chemical compound C1=CC(N)=CC=C1S(=O)(=O)NC1=NC=CC=N1 SEEPANYCNGTZFQ-UHFFFAOYSA-N 0.000 title claims abstract description 39
- 229960004306 sulfadiazine Drugs 0.000 title claims abstract description 39
- 208000015181 infectious disease Diseases 0.000 title claims abstract description 11
- 230000001850 reproductive effect Effects 0.000 title claims abstract description 10
- 241001465754 Metazoa Species 0.000 claims abstract description 19
- 241000238421 Arthropoda Species 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 11
- 241000256618 Trichogramma Species 0.000 claims description 23
- 241000244206 Nematoda Species 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 8
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 230000002708 enhancing effect Effects 0.000 claims description 5
- 238000011161 development Methods 0.000 claims description 4
- 230000018109 developmental process Effects 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 229940079593 drug Drugs 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 210000000805 cytoplasm Anatomy 0.000 claims description 3
- 230000008186 parthenogenesis Effects 0.000 claims description 3
- 230000003313 weakening effect Effects 0.000 claims description 3
- 230000001135 feminizing effect Effects 0.000 claims description 2
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 32
- 238000012545 processing Methods 0.000 description 22
- 229960003405 ciprofloxacin Drugs 0.000 description 16
- 230000017448 oviposition Effects 0.000 description 16
- 230000003115 biocidal effect Effects 0.000 description 15
- 230000032669 eclosion Effects 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 8
- 108010000912 Egg Proteins Proteins 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 6
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 235000013601 eggs Nutrition 0.000 description 6
- 210000004681 ovum Anatomy 0.000 description 6
- 241000993412 Corcyra cephalonica Species 0.000 description 4
- 241000239183 Filaria Species 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 230000003071 parasitic effect Effects 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 210000003128 head Anatomy 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000255777 Lepidoptera Species 0.000 description 2
- 208000030852 Parasitic disease Diseases 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- FDDDEECHVMSUSB-UHFFFAOYSA-N sulfanilamide Chemical compound NC1=CC=C(S(N)(=O)=O)C=C1 FDDDEECHVMSUSB-UHFFFAOYSA-N 0.000 description 2
- 229940124530 sulfonamide Drugs 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000289763 Dasygaster padockina Species 0.000 description 1
- 201000006353 Filariasis Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001477931 Mythimna unipuncta Species 0.000 description 1
- 241000346285 Ostrinia furnacalis Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000700141 Rotifera Species 0.000 description 1
- 241000985245 Spodoptera litura Species 0.000 description 1
- 241001414987 Strepsiptera Species 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 241001626749 Tetrastichinae Species 0.000 description 1
- 241000256680 Trichogrammatidae Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000010429 evolutionary process Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000003563 lymphoid tissue Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000004493 neutrocyte Anatomy 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000020509 sex determination Effects 0.000 description 1
- 210000004894 snout Anatomy 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/63—Compounds containing para-N-benzenesulfonyl-N-groups, e.g. sulfanilamide, p-nitrobenzenesulfonyl hydrazide
- A61K31/635—Compounds containing para-N-benzenesulfonyl-N-groups, e.g. sulfanilamide, p-nitrobenzenesulfonyl hydrazide having a heterocyclic ring, e.g. sulfadiazine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Agronomy & Crop Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Oncology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Communicable Diseases (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses sulphadiazines to the application in the T. pretiosum reproductive behavio(u)r of infection Wolbachia.The present invention provides sulphadiazines to remove animal parachorium Wolbachia or reduce the application in Wolbachia titre.Present invention firstly discovers that the hydromel containing 1.0mg/ml sulphadiazine, which is used alone, feeds T. pretiosum, can be good at removing the Wolbachia that T. pretiosum carries, this method is more convenient, time saving, and obtains holandry offspring.This will lay the foundation further to regulate and control arthropod mode of reproduction using Wolbachia, there is good application value and dissemination.
Description
Technical field
The present invention relates to arthropod Breeding behavior control technique field, more particularly, to sulphadiazine to infecting
Application in the T. pretiosum reproductive behavio(u)r of Wolbachia.
Background technique
Wolbachia is a kind of common intracellular symbiotic bacteria, is had in the arthropods such as insect, mite class and nematode higher
Infection rate.Studies have shown that thinks that the arthropod in nature has according to the data having been reported, by model estimation
66% has all infected Wolbachia.In long-term evolutionary process, Wolbachia has following modulate host mode of reproduction
Ability: lead to the incompatible cytoplasm of host, induced parthenogenesis, lead to feminizing, male killer, enhancing breeding for male gene
Power, to be conducive to itself existence and propagation, and there are negative impacts to the procreation of host.
Trichogramma (Trichogramma Westwood) is under the jurisdiction of a kind of parasitic insect of Hymenoptera Trichogrammatidae, red
It includes more than 180 kinds that eye bee belongs to altogether, is that most wide a kind of natural enemy insect is used in biological control.Trichogramma is egg parasitoids, children
Worm is parasitic in the ovum of moth class, therefore is used for biological control.Research report trichogramma is successfully used to prevent and treat various
Lepidoptera self-employed tree cultivator pest.Such as: corn field can parasitic corn borer, armyworm, snout moth's larva, bollworm, prodenia litura, cutworm and pine
The lepidoptera pests such as caterpillar.
The Sex Determination of trichogramma is list times/double figure.Under the conditions of no male and female mating, the drone of Haploid production.Have
Under the conditions of male and female mating, the queen bee of amphiploid is generated.But some trichogrammas can generate male lonely by producing after infecting Wolbachia
Female reproduction is changed into thelytoky.Due in production application, only raising trichogramma meeting of the queen bee than raising two kinds of genders
Save more production costs.And in field adaptability, the trichogramma with thelytoky ability is more conducive to establish field
Between population, therefore want to obtain a kind of Trichogramma strains that the stable female orphan of production is female always.Using Wolbachia to trichogramma reproduction
Regulation has more saving, more efficient advantage.In order to change in the intracorporal Wolbachia titre of trichogramma or removal trichogramma body
Wolbachia is to change the Breeding behavior of trichogramma, most common method is the utilization of high temperature (> 30 DEG C) and antibiotic.It is high
Temperature can reduce the intracorporal titre of trichogramma, lead to that the trichogramma of thelytoky generates drone in generation behind or female male phase is embedding
Body.But high temperature can generally also reduce the survival rate and egg laying amount of trichogramma.
Having infected Wolbachia T. pretiosum (Trichogramma pretiosum) is exactly to be turned by arrhenotocia
Become thelytoky.The genome of T. pretiosum and its Wolbachia genome felt in vivo are all public at present
Cloth, but Wolbachia is still to be studied to the regulatory mechanism of T. pretiosum thelytoky.The problem of one of most critical is
Obtain feminine gender is a series of necessity of researchs without the lonely female production hero T. pretiosum strain of Wolbachia is infected as negative control
Condition.It only obtains negative without infection Wolbachia T. pretiosum strain, is possible to research and understands Wolbachia
Switch to the mechanism of thelytoky reproductive behavio(u)r regulation phenomenon from lonely female production hero to T. pretiosum, and then utilizes Wolbachia pairs
Arthropod regulates and controls to control the Breeding behavior of arthropod.Report before has using tetracycline and high temperature respectively to more
Kind of the intracorporal Wolbachia of trichogramma is handled, and to eliminate its intracorporal Wolbachia, is obtained negative without infection
Wolbachia Trichogramma strains.But more multiple laboratories of people the experimental results showed that, tetracycline and high temperature can not be gone completely
Except the female production hero offspring of the orphan of the intracorporal Wolbachia of T. pretiosum or acquisition 100%.Therefore lack a kind of safe change at present
Become Wolbachia method in the intracorporal Wolbachia titre of trichogramma or removal trichogramma body, constrain correlative study into
Exhibition.
In addition, nematoda is a biggish monoid in the animal kingdom, it is distributed very wide, free living type is in seawater, light
Have in water, soil, some is food with algae, fungi etc., and some eats wheel animalcule etc.；Parasitic type colonizes in people, animal and plant
It is internal, endanger it is larger.Wherein, filaria (filarial worm) is arbo a kind of nematode, caused silk
Parasitosis refers to that filaria colonizes in parasitic disease caused by lymphoid tissue, subcutaneous tissue or serous cavity.Studies have shown that Wolbachia
It widely exists in filaria body, Wolbachia is necessary to the growth and development and reproduction of filaria；Wolbachia is also acted on
In mammalian hosts immune system, and by cause including neutrophil leucocyte, macrophage and T lymphocyte, including inflammation
Disease reaction and in the pathogenesis of filariasis correlation.Therefore it is beneficial to by the intracorporal Wolbachia of the online worm of cleaning symbiosis
Prevent and treat the parasitogenic animals and plants disease and disease due to nematode.
Summary of the invention
The purpose of the invention is to overcome the deficiencies of the prior art and provide sulphadiazine to infection Wolbach ia's
Application in T. pretiosum reproductive behavio(u)r.
The first purpose of the invention is to provide sulphadiazines to remove animal parachorium Wolbachia or reduction
Application in Wolbachia titre.
A second object of the present invention is to provide sulphadiazines to remove animal parachorium Wolbachia or reduction in preparation
Application in the drug of Wolbachia titre.
Third object of the present invention is to provide sulphadiazines to restore arthropod reproduction caused by symbiosis Wolbachia
Application in behavior change.
Fourth object of the present invention is to provide sulphadiazine and is eliminating or weakening the life of nematode caused by symbiosis Wolbachia
The promotion or humidification of long development and reproduction.
Fifth object of the present invention is to provide sulphadiazine in preparation without the application in infection Wolbachia animal.
After sixth object of the present invention is to provide a kind of to the T. pretiosum acquisition holandry for carrying Wolbachia
The method in generation,
To achieve the goals above, the present invention is achieved by the following technical programs:
The removal Wolbachia that inventor carries out the T. pretiosum for carrying Wolbachia using sulphadiazine, it is this
Method is more convenient, time saving, and obtains holandry offspring.
Therefore claimed:
Sulphadiazine is removing animal parachorium Wolbachia or is reducing the application in Wolbachia titre；
Sulphadiazine is removed in animal parachorium Wolbachia or the drug for reducing Wolbachia titre in preparation
Using.
Preferably, the animal is Arthropoda animal or Nemathelminthes animal.
Preferably, the arthropod is T. pretiosum.
Sulphadiazine is restoring the application in the change of arthropod reproductive behavio(u)r caused by symbiosis Wolbachia.
Preferably, the reproduction row, which is changed into, leads to the incompatible cytoplasm of host, induced parthenogenesis, leads to male gene
Feminize, male killer or enhancing one or more of reproductive capacity.
Preferably, the arthropod is T. pretiosum.
Sulphadiazine is in the promotion or enhancing for eliminating or weakening the development and reproduction of nematode growth caused by symbiosis Wolbachia
Effect.
Sulphadiazine is in preparation without the application in infection Wolbachia animal.
Preferably, the animal is Arthropoda animal or Nemathelminthes animal.
Preferably, the arthropod is T. pretiosum.
The method that the T. pretiosum of a kind of couple of carrying Wolbachia obtains holandry offspring, raises T. pretiosum
Feed sulphadiazine.
Preferably, no less than for 24 hours using the hydromel feeding T. pretiosum containing 1.0mg/ml sulphadiazine.
Compared with prior art, the invention has the following beneficial effects:
The present invention is used alone the hydromel containing 1.0mg/ml sulphadiazine and feeds T. pretiosum, finds energy of the present invention
There is higher infection rate in the arthropods such as enough insect, mite class of the removal well including T. pretiosum and nematode
Interior raw Wolbachia, this method is more convenient, time saving, and T. pretiosum is made to obtain holandry offspring, eliminates
Influence of the Wolbachia to host.This will lay the foundation further to regulate and control arthropod mode of reproduction using Wolbachia,
There are good application value and dissemination.
Specific embodiment
The present invention is made below with reference to specification specific embodiment and further being elaborated, the embodiment is served only for
It explains the present invention, is not intended to limit the scope of the present invention.Test method as used in the following examples unless otherwise specified,
It is conventional method；Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
1, for trying insect
Test T. pretiosum population and be purchased from the U.S., by exterior paramera feature carry out form and ITS2 gene sequencing into
Row Molecular Identification (Stouthamer et al., 1998).Intracorporal Wolbachia is expanded and is surveyed using wsp special primer
Sequence identifies (Baldo et al.2006).25 DEG C, relative humidity 70%-80%, photoperiod 16L in constant incubator:
Rice moth egg Corcyra cephalonica breeding oophagous trichogrammae conservation is used under conditions of 8D, excessive generation is raised steady in this laboratory
Fixed breeding.
2, preparation of reagents
Ciprofloxacin, sulphadiazine are purchased from Jian Yang biotech firm.Utilize two kinds of antibiotic ciprofloxacins and sulphadiazine
Three concentration handle T. pretiosum, and Ciprofloxacin, Ciprofloxacin: sulphadiazine=1:1, sulphadiazine are distinguished
0.1g/ml, 10mg/ml, 10mg/ml antibiotic hydromel are diluted to 30% hydromel.
Embodiment 1
One, experimental method
The T. pretiosum queen bee just to have sprouted wings is taken out with small writing brush under stereoscope, single head is put into dactylethrae, feeds respectively
Three kinds of antibiotic hydromels, and control group is set, the fresh rice moth egg that feeding is put into no less than 100 embryos that gone out afterwards for 24 hours sticks into
Row is parasitic.Parasitism chooses trichogramma with small writing brush afterwards for 24 hours and is put into centrifuge tube in -30 DEG C of preservations, and ovum card, which is put into dactylethrae, to be continued to put
Enter climate box culture, 25 DEG C of temperature, relative humidity 80%~85%, illumination condition L:D=14:10.Three groups of weights are done in each processing
It is multiple.In 2 generation of continuous processing, records per generation processing rice moth egg blackening number i.e. trichogramma egg laying amount, female, male and male and female mosaics
Number calculates eclosion rate, female and male ratio.
The calculating of egg laying amount: the ovum grain number of discoloration (black) is female as single head on every ovum card under the meter record in the 7th day after connecing bee
The yield ovum of bee.
The calculating of eclosion rate: when occurring in small test tube into bee start recording all emergence into bee head number.
Eclosion rate %=is used as eclosion rate at bee number/egg laying amount × 100.
Produce the calculating of male rate: after the bee in per generation is dead, under 100 times of stereoscopes according to feeler morphological feature and abdomen whether
Distinguish male and female with oopod: Antennae of Male Endoparasitoid Hymenoptera Tetrastichinae hair is longer, wherein longest antennal hair is 2.5 times of flagellum the widest part, as
The primary outer morphological feature of male.Queen bee antennal hair is shorter, and abdomen has oopod.
Produce emergence number of individuals × 100 of male rate %=male number/always.
Two, experimental result
1, influence of the antibiotic to T. pretiosum egg laying amount
From the point of view of the analysis result of table 1, the eggs on average amount in F0 generation is 18.67~47.67, the eggs on average amount of F1 generation
It is 27.00~36.00.In addition to 10.0g/ml Ciprofloxacin: sulphadiazine=1: in 1 processing, the egg laying amount ratio F0 generation of F1 generation
Egg laying amount it is significant want other than height, egg laying amount is not significantly different between other processing different generations.Right page comes from overall trend
It sees, the egg laying amount in the egg laying amount ratio F0 generation of F1 generation is high.
In the result in F0 generation, it is used alone and the low processing egg laying amount specific concentration height of concentration is presented in Ciprofloxacin processing result
The higher egg laying amount of processing.Also, compared with the control group, the Ciprofloxacin processing egg laying amount of 0.1g/ml and 1g/ml has height
Egg laying amount, significant difference.And Ciprofloxacin: sulphadiazine=1: 1 and six of sulphadiazine processing in, produce compared with the control
Ovum amount difference is not significant.
In the result of F1 generation, compared with the control, egg laying amount all difference after all antibiotic treatments is not significant.
Table 1: influence of the antibiotic to T. pretiosum egg laying amount:
2, influence of the antibiotic to T. pretiosum eclosion rate
From the point of view of the analysis result of table 2, in addition to the eclosion rate of the Ciprofloxacin processing of 0.1g/ml in the F2 generation
(75.86%) it is lower than significantly outside the eclosion rate of F1 generation, eclosion rates of other processing between different generations are not significantly different.
From the point of view of same generation difference antibiotic treatment result, compared with the control, all processing eclosion rate differences of F1 generation are not
Significantly, reach 77.73% or more.In F2 generation, the eclosion rate of 10mg/ml sulphadiazine and the processing of 10mg/ml Ciprofloxacin is aobvious
It is higher than 0.1mg/ml Ciprofloxacin: sulphadiazine=1: 1 eclosion rate with writing (respectively 95.38% and 95.96%)
(75.86%), but compared with control treatment, difference is not significant.The results show that only in 0.1mg/ml Ciprofloxacin: sulfanilamide (SN) is phonetic
Pyridine=1: the eclosion rate that F2 generation is embodied in 1 processing is more significant than the eclosion rate of F1 generation relatively low outer, others processing or higher concentration
Antibiotic treatment between different generations compared to there is not the significant difference that changes.
Influence of 2 antibiotic of table to T. pretiosum eclosion rate:
3, influence of the antibiotic to T. pretiosum sex of children
Antibiotic can find out (table 3) in the influence to the male rate of T. pretiosum production, and in F1 generation, all processing are all never
There is male.Since F2 generation, processing (the 0.1mg/ml Ciprofloxacin: sulphadiazine=1: 1 He of low antibiotic concentration
The processing of 0.1mg/ml sulphadiazine) start male occurred, and work as concentration and reach in the processing of 1.0g/ml or more
100% male offspring is showed.Also, the Ciprofloxacin of 0.1mg/ml: it is embedding sulphadiazine=1: to there is female male phase in 1 processing
Body.But Ciprofloxacin still fails to obtain the offspring of male by the screening in 6 generations.The results show that using only Ciprofloxacin
Processing cannot obtain male；It is mixed with sulphadiazine to taking using 1.0mg/ml sulphadiazine or using Ciprofloxacin
After the red bee of the short tube of band is handled, 100% male has just been had already appeared in F2 generation.This shows that sulphadiazine can have
The effect ground removal intracorporal Wolbachia of T. pretiosum, to reach the generation of 100% male offspring.
3 antibiotic of table produces the influence of male rate to T. pretiosum:
Claims (10)
1. sulphadiazine is removing animal parachorium Wolbachia or is reducing the application in Wolbachia titre.
2. sulphadiazine removes answering in animal parachorium Wolbachia or the drug for reducing Wolbachia titre in preparation
With.
3. application according to claim 1 or 2, which is characterized in that the animal is that Arthropoda animal or nematode are dynamic
Object door animal.
4. sulphadiazine is restoring the application in the change of arthropod reproductive behavio(u)r caused by symbiosis Wolbachia.
5. application according to claim 4, which is characterized in that the reproduction row, which is changed into, leads to the cytoplasm of host not phase
Appearance, induced parthenogenesis one or more of lead to the feminizing of male gene, male killer or enhancing reproductive capacity.
6. sulphadiazine is made in the promotion or enhancing for eliminating or weakening the development of nematode growth caused by symbiosis Wolbachia and reproduction
With.
7. sulphadiazine is in preparation without the application in infection Wolbachia animal.
8. sulphadiazine is in the application of the drug of preparation disease as caused by nematosis or disease.
9. a kind of method for obtaining holandry offspring to the T. pretiosum for carrying Wolbachia, which is characterized in that short tube
Trichogramma feeds sulphadiazine.
10. according to the method described in claim 9, it is characterized in that, being raised using the hydromel containing 1.0mg/ml sulphadiazine
Feed T. pretiosum no less than for 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910301264.3A CN109997794B (en) | 2019-04-15 | 2019-04-15 | Application of sulfadiazine in reproduction behavior of Wolbachia infected short-tube trichogramma |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910301264.3A CN109997794B (en) | 2019-04-15 | 2019-04-15 | Application of sulfadiazine in reproduction behavior of Wolbachia infected short-tube trichogramma |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109997794A true CN109997794A (en) | 2019-07-12 |
| CN109997794B CN109997794B (en) | 2021-10-29 |
Family
ID=67172096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910301264.3A Active CN109997794B (en) | 2019-04-15 | 2019-04-15 | Application of sulfadiazine in reproduction behavior of Wolbachia infected short-tube trichogramma |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109997794B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150104492A1 (en) * | 2013-10-14 | 2015-04-16 | Alison McDermott | Esculentin 1a Derivatives and Uses Thereof |
| CN104837860A (en) * | 2012-09-07 | 2015-08-12 | 新加坡科技研究局 | Peptides and their uses |
| WO2018141063A1 (en) * | 2017-02-02 | 2018-08-09 | Mcmaster University | Bicarbonate as a potentiator for antimicrobial agents |
| CN109072280A (en) * | 2016-01-21 | 2018-12-21 | 赛录科试诊断公司 | Method for rapid antimicrobial agent sensitivity tests |
-
2019
- 2019-04-15 CN CN201910301264.3A patent/CN109997794B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104837860A (en) * | 2012-09-07 | 2015-08-12 | 新加坡科技研究局 | Peptides and their uses |
| US20150104492A1 (en) * | 2013-10-14 | 2015-04-16 | Alison McDermott | Esculentin 1a Derivatives and Uses Thereof |
| CN109072280A (en) * | 2016-01-21 | 2018-12-21 | 赛录科试诊断公司 | Method for rapid antimicrobial agent sensitivity tests |
| WO2018141063A1 (en) * | 2017-02-02 | 2018-08-09 | Mcmaster University | Bicarbonate as a potentiator for antimicrobial agents |
Non-Patent Citations (1)
| Title |
|---|
| 彭建新等: "《昆虫分子遗传学》", 30 November 2006 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109997794B (en) | 2021-10-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101720705B (en) | Culture method of orange adductor scallop | |
| CN103141443B (en) | A kind of regulate and control the method for beet webworm Ah lattice ichneumon wasp diapause | |
| CN106922583A (en) | A kind of Macrobrachium rosenbergii SPF（It is virus-free）Offspring breeding method | |
| CN102150644B (en) | Breeding method for antitumor chrysomyia megacephala larvae | |
| WO2020060382A1 (en) | Method of producing biomass of galleria mellonella larvae | |
| Fei et al. | Comparative study of incipient colony development in the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera, Rhinotermitidae) | |
| Ovie et al. | The effect of different algal densities of Scenedesmus acuminatus on the population growth of Moina micrura Kurz (Crustacea: Anomopoda, Moinidae) | |
| CN106577372B (en) | A kind of overwintering and artificial fecundation method of sea otter | |
| CN108935341B (en) | Method for raising cabbage butterfly velvet cocoon bee | |
| CN101361474B (en) | Preparation method of silkworm commercial race transgene silkworm egg | |
| CN102960276A (en) | Cultivation method of Chlamys nobilis new strain enriched with DNA (Deoxyribonucleic Acid) and application thereof | |
| CN105274007B (en) | One plant of Metarhizium anisopliae var. Anisopliae MaTS02 and its application in terms of preventing and treating Bemisia tabaci | |
| CN1463595A (en) | Method for cultivating scallop hybrids | |
| CN109997794A (en) | Sulphadiazine is to the application in the T. pretiosum reproductive behavio(u)r of infection Wolbachia | |
| Fitt | Nutrition of giant clams | |
| CN115428770A (en) | Method for adjusting egg laying time of ladybug by using alternative feed | |
| CN107810930A (en) | A kind of method for breeding of Serangium japonicum | |
| Ogah et al. | Parasitism and development of Platygaster diplosisae (Hymenoptera: Platygastridae) on the African rice gall midge Orseolia oryzivora (Diptera: Cecidomyiidae) | |
| Baverstock et al. | Potential value of the fibre nettle Urtica dioica as a resource for the nettle aphid Microlophium carnosum and its insect and fungal natural enemies | |
| CN111727936A (en) | Indoor propagation method of chilo suppressalis fringed cocoon bee | |
| Balog | Jumping–ship–can have its costs: implications of predation and host plant species for the maintenance of pea aphid (Acyrthosiphon pisum Harris) colour polymorphism | |
| Hodosh et al. | Toxicological evaluation and registration requirements for biorational pesticides | |
| CN105309391A (en) | Novel indoor feeding method for spiders | |
| Clough et al. | Variability of inbred and incross chickens | |
| CN114642191B (en) | Method for hybridization between different geographic populations of pod moths |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |