CN109982707A - 血管性血友病因子(vwf)靶向剂及其使用方法 - Google Patents

血管性血友病因子(vwf)靶向剂及其使用方法 Download PDF

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Publication number
CN109982707A
CN109982707A CN201780069446.1A CN201780069446A CN109982707A CN 109982707 A CN109982707 A CN 109982707A CN 201780069446 A CN201780069446 A CN 201780069446A CN 109982707 A CN109982707 A CN 109982707A
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aptamer
rna
synthetic
polynucleotide
seq
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CN201780069446.1A
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Chinese (zh)
Inventor
S·M·尼姆治
G·A·皮托克
J·雷泽尔
布鲁斯·撒兰格
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Ohio State Innovation Foundation
Duke University
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Ohio State Innovation Foundation
Duke University
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    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • A61K31/7105Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
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CN201780069446.1A 2016-09-16 2017-09-18 血管性血友病因子(vwf)靶向剂及其使用方法 Pending CN109982707A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662395642P 2016-09-16 2016-09-16
US62/395,642 2016-09-16
PCT/US2017/052063 WO2018053427A1 (en) 2016-09-16 2017-09-18 Von willebrand factor (vwf)-targeting agents and methods of using the same

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CN109982707A true CN109982707A (zh) 2019-07-05

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US (3) US10889816B2 (https=)
EP (1) EP3512526A4 (https=)
JP (2) JP7560821B2 (https=)
CN (1) CN109982707A (https=)
WO (1) WO2018053427A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016176426A1 (en) 2015-04-28 2016-11-03 Duke University Thrombus imaging aptamers and methods of using same
CA3095080A1 (en) * 2018-03-27 2019-10-03 Coen MAAS Targeted thrombolysis for treatment of microvascular thrombosis
JP2022044843A (ja) * 2018-12-11 2022-03-18 日産化学株式会社 解毒剤
US20230038761A1 (en) * 2020-02-04 2023-02-09 Band Therapeutics, Llc Regulation of von willebrand factor (vwf)
CN113244381A (zh) * 2021-06-02 2021-08-13 苏州大学 游离的血红蛋白及其衍生物抗血小板聚集的应用
WO2025076424A1 (en) * 2023-10-06 2025-04-10 Ohio State Innovation Foundation Aptamer bb-031 and methods of using thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040171114A1 (en) * 2002-09-23 2004-09-02 Steven Gutteridge Isolation and use of ryanodine receptors
US20090264508A1 (en) * 2005-09-15 2009-10-22 Sullenger Bruce A Focused Libraries, Functional Profiling, Laser Selex, and Deselex
US20120264815A1 (en) * 2006-10-19 2012-10-18 Duke University Reversible platelet inhibition

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5770198A (en) 1988-05-18 1998-06-23 The Research Foundation Of The State Of New York Platelet-specific chimeric 7E3 immunoglobulin
US6261774B1 (en) 1990-06-11 2001-07-17 Gilead Sciences, Inc. Truncation selex method
US5660985A (en) 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5756291A (en) 1992-08-21 1998-05-26 Gilead Sciences, Inc. Aptamers specific for biomolecules and methods of making
JPH08502966A (ja) 1992-10-23 1996-04-02 ユニバーシティー・オブ・マサチューセッツ・メディカル・センター Rna/リガンド結合の低分子抑制
US7396664B2 (en) 1999-06-08 2008-07-08 Regeneron Pharmaceuticals, Inc. VEGF-binding fusion proteins and nucleic acids encoding the same
US6780850B1 (en) 1999-06-22 2004-08-24 Triumf Extending the lifetime of anticoagulant oligodeoxynucleotide aptamers in blood
GB0014136D0 (en) 2000-06-10 2000-08-02 Astrazeneca Ab Combination therapy
GB0014134D0 (en) 2000-06-10 2000-08-02 Astrazeneca Ab Combination therapy
US6376190B1 (en) 2000-09-22 2002-04-23 Somalogic, Inc. Modified SELEX processes without purified protein
US7312325B2 (en) 2000-09-26 2007-12-25 Duke University RNA aptamers and methods for identifying the same
DK2070939T3 (da) 2001-05-25 2014-06-23 Univ Duke Modulatorer af farmakologiske midler
AUPR604101A0 (en) 2001-06-29 2001-07-26 Unisearch Limited Aptamers
US20070071744A1 (en) 2002-06-07 2007-03-29 Gotz Munch Agents which bind to epitopes of glycoprotein VI
US20060193821A1 (en) 2004-03-05 2006-08-31 Diener John L Aptamers to the human IL-12 cytokine family and their use as autoimmune disease therapeutics
EP2460811A1 (en) 2004-04-22 2012-06-06 Regado Biosciences, Inc. Improved modulators of coagulation factors
EP1789096A4 (en) 2004-09-07 2009-07-08 Archemix Corp APTAMERS FOR THE VON WILLEBRAND FACTOR AND ITS USE AS THERAPEUTICS FOR THROMBOTIC DISEASES
US7566701B2 (en) 2004-09-07 2009-07-28 Archemix Corp. Aptamers to von Willebrand Factor and their use as thrombotic disease therapeutics
KR20070101226A (ko) 2004-09-07 2007-10-16 아케믹스 코포레이션 앱타머의 약화학
TWI403334B (zh) 2004-12-23 2013-08-01 Merck Sharp & Dohme 包含生物素殘基之抗血栓雙重抑制劑
US7795009B2 (en) 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
US20100076060A1 (en) 2005-09-15 2010-03-25 Duke University Aptamers as agonists
RU2568579C2 (ru) 2006-05-26 2015-11-20 ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "НоваМедика" Введение противосвертывающей системы reg1
US20110118187A1 (en) 2006-10-19 2011-05-19 Duke University Reversible platelet inhibition
US8372427B2 (en) 2007-03-05 2013-02-12 Abbott Cardiovascular Systems Inc. Therapeutic composition with enhanced endothelium targeting
EP2139992A4 (en) 2007-03-30 2011-08-10 Univ Duke METHOD FOR MODULATING THE ACTIVITY OF A NUCLEIC ACID MOLECULE
WO2008150495A2 (en) * 2007-06-01 2008-12-11 Archemix Corp. Vwf aptamer formulations and methods for use
WO2010020423A2 (en) 2008-08-22 2010-02-25 Baxter Healthcare S.A. Methods for treating bleeding disorders
JP2012528597A (ja) 2009-06-03 2012-11-15 レガド・バイオサイエンシズ・インク 血小板グリコプロテインviの核酸調節因子
KR101766408B1 (ko) 2009-06-10 2017-08-10 알닐람 파마슈티칼스 인코포레이티드 향상된 지질 조성물
IN2012DN02345A (https=) 2009-09-16 2015-08-21 Univ Duke
WO2012050611A2 (en) 2010-10-13 2012-04-19 Duke University Aptamers to glycoprotein vi
US9150867B2 (en) * 2011-04-28 2015-10-06 Mayo Foundation For Medical Education And Research DNA aptamers for promoting remyelination
US9687529B2 (en) 2011-05-05 2017-06-27 Duke University Method of controlling coagulation
WO2013043902A1 (en) 2011-09-21 2013-03-28 Nplex Laboratory Inc. Targeted nanoparticles joined to reporter molecules through multiple mechanisms
EP4170031A1 (en) * 2012-10-23 2023-04-26 Caris Science, Inc. Aptamers and uses thereof
WO2014169049A1 (en) * 2013-04-09 2014-10-16 Duke University 2' fluoro-modified rnas as immunostimulators
WO2014193800A2 (en) * 2013-05-28 2014-12-04 The Johns Hopkins University Aptamers for the treatment of sickle cell disease
KR20170055474A (ko) 2014-07-31 2017-05-19 아카데미아 시니카 길항 pd-1 압타머 및 암 요법 관련 적용에서의 그의 적용
WO2016176426A1 (en) 2015-04-28 2016-11-03 Duke University Thrombus imaging aptamers and methods of using same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040171114A1 (en) * 2002-09-23 2004-09-02 Steven Gutteridge Isolation and use of ryanodine receptors
US20090264508A1 (en) * 2005-09-15 2009-10-22 Sullenger Bruce A Focused Libraries, Functional Profiling, Laser Selex, and Deselex
US20120264815A1 (en) * 2006-10-19 2012-10-18 Duke University Reversible platelet inhibition

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