CN109975564A - A kind of integrated reagent automatic detection device of microfluid immunoassay - Google Patents
A kind of integrated reagent automatic detection device of microfluid immunoassay Download PDFInfo
- Publication number
- CN109975564A CN109975564A CN201910217642.XA CN201910217642A CN109975564A CN 109975564 A CN109975564 A CN 109975564A CN 201910217642 A CN201910217642 A CN 201910217642A CN 109975564 A CN109975564 A CN 109975564A
- Authority
- CN
- China
- Prior art keywords
- sample
- luminous substrate
- reagent
- liquid
- substrate liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention relates to a kind of microfluid immunoassays to integrate reagent automatic detection device, it may include working rotary table, automatic sampling apparatus, luminous substrate liquid filling apparatus, hematocrit scale reading device and fluorescence/luminous detection unit, working rotary table is divided into even number reagent position, on each reagent position can one microfluid immunoassay of fixed placement integrate reagent device, a plurality of hematocrit test pipe jigs are symmetrically arranged on working rotary table, hematocrit testing tube is placed wherein, automatic sampling apparatus is used to for blood sample to be detected to be added to microfluid immunoassay and integrate in reagent device, luminous substrate liquid filling apparatus is for luminous substrate liquid to be filled into characteristic indication object reaction tube, hematocrit scale reading device is used to read the hematocrit number after being centrifuged in hematocrit testing tube, fluorescence/the detection unit that shines is used Sample after integrating the reaction and washing in reagent device to microfluid immunoassay carries out fluorescence/luminous detection.
Description
Technical field
The present invention relates to a kind of servos to detect the constructional device that integrated Reagent Tube detects automatically immediately, specifically a kind of to cure
It treats in diagnosis, health supervision, food safety and other emergency places and realizes the highly sensitive microfluid immunoassay detected immediately
Integrated reagent automatic detection device.
Background technique
In medical diagnosis, health supervision, food safety and other emergency places, highly sensitive instant detection is automated always
There is the thriving demand of reality.
Detect and be widely used immediately currently based on the labelled immune of lateral chromatography, marker be related to colloidal gold,
Fluorescence, lanthanide series rare-earth elements Eu2+, quantum dot etc..Its maximum advantage is to realize to detect easy to operate, instrument structure phase
To brief, it is able to satisfy many places and detects needs immediately.Since the quality of the labelled immune reagent material based on lateral chromatography can
Poor, controlling of production process the uncertainty of control property is big, reagent assembling material property is unstable and the uncontrollability of detection process, inspection
Surveying result, that there are sensitivity is low, variation is big, and clinical application is easy to situations such as failing to pinpoint a disease in diagnosis, mistaken diagnosis generation occur, so be only used for high-risk
The screening of crowd detects.
The instant detection of other forms include the detection of magnetic particle solid state chemistry electrochemiluminescent immunoassay, micro-fluidic drive mode it is instant
Detection etc., in the same period also in development and application.However in the label of the terseness of analysis and convenience compared with lateral chromatography
Immune detection immediately comes complicated, and reagent, instrument composition are required to more operation and subsidiary conditions.Unquestionably, Ren Men
The method that the labelled immune of sensitiveer, easy similar lateral chromatography detects such simplicity immediately is expected, to meet real needs
Demand.
Summary of the invention
The present invention is directed to propose a kind of microfluid immunoassay integrate reagent automatic detection device, using capillarity and from
The driving and completion detection that the reaction process of reagent device is integrated to a kind of microfluid immunoassay are realized in mental and physical efforts driving, and fixed
Property, quantifying reporter result.Particularly, it is able to achieve automatic washing detection pipe, makes to have reacted and is separated with unreacted component, is greatly improved
Detection sensitivity；By the hematocrit or hematocrit ratio of each sample of survey calculation, will test to be measured in whole blood sample
The concentration of ingredient is accurately converted into plasma concentration.
To achieve the above object, the present invention provides a kind of integrated reagent automatic detection device of microfluid immunoassay, special
Sign is: including working rotary table, automatic sampling apparatus, luminous substrate liquid filling apparatus, hematocrit scale reading device and glimmering
Light/shine detection unit, the working rotary table is divided into even number reagent position, on each reagent position can fixed placement one it is micro-
Fluid immunoassay integrates reagent device, and a plurality of hematocrit test pipe jigs, institute are symmetrically arranged on the working rotary table
It states hematocrit test pipe jig and is arranged to make to be placed on hematocrit testing tube therein and revolved in the working rotary table
Become horizontal position from upright position when turning to complete whole blood haemocyte centrifugation, the automatic sampling apparatus is arranged in sample introduction
By station, for blood sample to be detected to be added to the integrated reagent device of the microfluid immunoassay for going to the sample introduction station
In, blood sample to be detected integrates in reagent device in the microfluid immunoassay to be reacted and is washed, the luminous substrate liquid
Filling apparatus is arranged in by luminous substrate liquid filling station, is gone to the luminous substrate liquid for luminous substrate liquid to be filled into and is added
In the characteristic indication object reaction tube for infusing station, the hematocrit scale reading device is arranged in outside the working rotary table
On fixed position, for reading the hematocrit number after centrifugation in hematocrit testing tube, the fluorescence/shine detection list
Member is arranged in detection station, anti-in reagent device for integrating to the microfluid immunoassay for going to the detection station
Fluorescence/luminous detection should be carried out with the sample after washing.
Further, it includes pedestal, upper cover and characteristic indication analyte detection that the microfluid immunoassay, which integrates reagent device,
Reaction tube is successively arranged washing lotion storage tank, marker film loading drain structure, reaction tube peace along reagent flow direction on the pedestal
Seat and liquid waste tank are filled, the upper cover is in conjunction with the pedestal inserted type, and the characteristic indication analyte detection reaction tube is by PS plastic
It is molded, fixation is embedded in the reaction pipe mounting seat, and has slit-shaped inner cavity, anti-to promote to generate capillarity
Answer process, the both ends of the inner cavity are respectively inlet and liquid outlet, and the marker film loading drain structure is by marker film
It is fixedly mounted on the washing lotion introducing port and Sample introduction mouth that in the inlet and there is liquid guiding cavity and be connected to the liquid guiding cavity,
The washing lotion storage tank is in fluid communication by the washing lotion introducing port and the liquid guiding cavity, the liquid guiding cavity and the inlet fluid
Connection, the liquid waste tank and the liquid outlet are in fluid communication, and it is filled with water-absorbent materials.
Further, the washing lotion storage tank is an oval cavity body structure, is inside placed with sealing washing lotion bag, and court
It is equipped with opening to marker film loading drain structure side, which is in fluid communication and built-in with the washing lotion introducing port
There is pricker, the pricker is for puncturing washing lotion bag.
Still further, the washing lotion volume in the washing lotion bag is 1 milliliter.
Further, the marker film loading drain structure includes marker film fixing card, and the marker film is solid
Block the marker film position for marker film to be pushed to and is fixed on the inlet calmly.
Further, the Sample introduction mouth is that cross binds out closing structure, and sample introduction needle imports sample after opening, into
Sample needle exits after-opening closure.
Further, the liquid waste tank is in fluid communication by a mouth of a gorge and the liquid outlet.
Further, the characteristic indication object reaction tube is provided with the importing of luminous substrate liquid at the liquid outlet
Hole, the luminous substrate liquid entrance hole is for being added luminous substrate liquid.
Still further, the luminous substrate liquid filling apparatus includes luminous substrate liquid bottle, luminous substrate fluid catheter, hair
Light substrate solution dispenser pump, the mobile motor of luminous substrate liquid filling needle and luminous substrate liquid fill needle, the luminous substrate fluid catheter
The luminous substrate liquid bottle and luminous substrate liquid filling needle are connected, the luminous substrate liquid dispenser pump is mounted on described shine
On substrate fluid catheter, the mobile motor of luminous substrate liquid filling needle is for moving the luminous substrate liquid filling needle.
Further, the reaction pipe mounting seat is equipped with edge, and the edge is for fixing the characteristic indication object
Reaction tube.
Further, the circular in cross-section, rectangle of the inner cavity of the characteristic indication object reaction tube, semicircle or
Ellipse.
Further, arc or wave structure can be equipped on the wall surface of the inner cavity, to increase the wall surface face of inner cavity
Product.
Further, the detection unit includes detector and excitation light source, the light projection of the excitation light source transmitting
Fluorescence is issued with provocative reaction object in the characteristic indication analyte detection reaction tube, the detector is for detecting the feature mark
The fluorescence of reactant in will analyte detection reaction tube/shine.
Still further, the detector is PMT detector.
Further, the automatic sampling apparatus includes sample cell fixed ring, sample cell rotation mixing driving structure, sample
Bar code reading record device, sample introduction needle, sample introduction needle support frame and sample introduction needle actuating device, the sample cell fixed ring are mounted on the sample
Pipe rotation mixes in driving structure and is driven by it rotation with the blood in the sample cell that will be fixed in the sample cell fixed ring
Liquid is uniformly mixed, and the sample bar code reading record device is used to read bar code and the upload on the sample cell, the sample introduction needle installation
On the sample introduction needle rack, the sample introduction needle actuating device drives the sample introduction needle for driving the sample introduction needle rack
It moves to sampling position, be loaded position and washing position, and then the sample introduction needle is made to implement sampling, sample-adding and wash certainly
Into one, the hematocrit test pipe jig has C-shaped cross-section, and upper surface is equipped with card slot, described
The cylindrical projections of a pair of of radial symmetric are equipped at the nozzle of hematocrit testing tube, the cylindrical projections are rotationally blocked
It connects in the card slot.
The present invention by adopting the above technical scheme, has the beneficial effect that
1. reaction process is controlled using capillarity and controllable rotating speed using high s/n ratio PS plastic as solid phase carrier,
Sufficiently, completely, detection is accurate, quantitative reliable for reaction.
2. the washing to reaction tube can be automatically performed, sensitivity is significantly improved.
3. hematocrit can be detected simultaneously, it is more acurrate to report corresponding plasma concentration result.
4. apparatus structure is simple, high degree of automation, multiple determination, any person-portion combine detection, flux is high.
Detailed description of the invention
To further illustrate that each embodiment, the present invention are provided with attached drawing.These attached drawings are that the invention discloses one of content
Point, mainly to illustrate embodiment, and the associated description of specification can be cooperated to explain the operation principles of embodiment.Cooperation ginseng
These contents are examined, those of ordinary skill in the art will be understood that other possible embodiments and advantages of the present invention.In figure
Component be not necessarily to scale, and similar component symbol is conventionally used to indicate similar component.
Fig. 1 is the structural schematic diagram that a kind of microfluid immunoassay of the invention integrates reagent automatic detection device；
Fig. 2 is the structural schematic diagram of the integrated reagent device of microfluid immunoassay in Fig. 1；
Fig. 3 is the structural schematic diagram of the characteristic indication analyte detection reaction tube in Fig. 2；
Fig. 4 a, 4b, 4c and 4d respectively illustrate the varying cross-section of characteristic indication analyte detection reaction tube described in Fig. 3.
Specific embodiment
Now in conjunction with the drawings and specific embodiments, the present invention is further described.
As shown in Figure 1, a kind of microfluid immunoassay integrate reagent automatic detection device may include working rotary table 1, it is automatic
Sampling device 2, luminous substrate liquid filling apparatus 3, fluorescence/detection unit 4 that shines and hematocrit scale reading device 7.Below
Working rotary table 1, automatic sampling apparatus 2, luminous substrate liquid filling apparatus 3 and fluorescence/detection unit 4 that shines are carried out specifically respectively
Explanation.
Working rotary table 1 may include the shaft 11 by the controllable drive motor driving of program and be fixedly mounted on the shaft 11
On spoke disk 12.Spoke disk 12 is integrally formed production by engineering plastics, above equidistant subregion even number reagent position is set
121.Corresponding region outside spoke disk 12 is equipped with sample introduction station A, luminous substrate liquid fills station B and detection station C.Reagent position
121, equipped with the structure agreed with is inlayed with the integrated reagent device 5 of microfluid immunoassay, integrate loading microfluid immunoassay
Closely agree with fixation when reagent device 5 therewith.That is, can the integrated examination of one microfluid immunoassay of fixed placement on each reagent position
Agent device 5.For the sake of clarity, two microfluid immunoassays are only shown in Fig. 1 and integrate reagent device 5.Loading microfluid
It is off-loadable after detection project completion detection on the integrated reagent device 5 of immunoassay, and new loading miniflow to be detected is installed
Body immunoassay integrates reagent device 5, carries out next round detection operation.It loads microfluid immunoassay and integrates reagent device 5
Structure will be described in detail hereinafter.It is distributed on spoke disk 12 to 122 symmetry of hematocrit testing tube position, each blood
Specific volume of cell testing tube position 122 (does not show equipped with the hematocrit test pipe jig for 6 loading of hematocrit testing tube
Out), the hematocrit test pipe jig is arranged to make to be placed on hematocrit testing tube 6 therein in the work
Become horizontal position from upright position when turntable rotates to complete whole blood haemocyte centrifugation.Specifically, the haemocyte ratio
Holding test pipe jig has C-shaped cross-section, and upper surface is equipped with card slot, sets at the nozzle of the hematocrit testing tube 6
There are the cylindrical projections 61 of a pair of of radial symmetric, the cylindrical projections 61 are rotationally connected in the card slot.That is, described
Cylindrical projections 61 are used as shaft, so that hematocrit testing tube 6 can be gone under the influence of centrifugal force from upright position
Horizontal position, and the centrifugation of whole blood haemocyte is completed under the influence of centrifugal force.
Hematocrit scale reading device 7 is arranged on the fixation position outside the working rotary table, after reading centrifugation
Hematocrit number in hematocrit testing tube 6.The specific structure of hematocrit scale reading device 7 is art technology
Known to personnel, it is not further described herein.
Automatic sampling apparatus 2 is arranged in by sample introduction station A, it may include sample cell fixed ring 21, sample cell rotation are mixed and driven
Dynamic structure 22, sample bar code reading record device 23, sample introduction needle 24, sample introduction needle support frame 25 and sample introduction needle actuating device 26.Sample cell is solid
Determine ring 21 to be mounted in sample cell rotation mixing driving structure 22 and rotation can be driven by it.Sample cell fixed ring 22 is by sample cell
27, which are securely fastened to sample cell rotation, mixes in the shaft of driving structure 22, to ensure take off when rotation mixes and samples
It falls.Sample cell rotation mixes driving structure 22 for mixing before sampling to whole blood sample, to guarantee samples' representativeness.
Sample cell 27 holds the samples such as whole blood, serum or blood plasma, posts bar code thereon, can form one-to-one relationship with sample message,
For identification.When sample cell 27 loads, this bar code should be placed towards sample bar code reading record device 23, so that sample bar code reading is recorded
Device 23 can scan the bar code on sample cell 27.Sample bar code reading record device 23 is connected with host computer.Due to bar code and sample message
And index linking to be checked, therefore, host computer records the bar code information that device 23 uploads by sample bar code reading to control and index to be checked
The sampling number and each sampling amount of corresponding sample introduction needle 24.Sample introduction needle 24 is mounted on sample introduction needle rack 25, sample introduction needle driving
Device 26 by the driving of sample introduction needle 24 to sampling position, sample-adding position and washing position, and then makes for driving the sample introduction needle rack 25
The sample introduction needle is implemented sampling, sample-adding and is washed certainly.
As in Figure 2-4, it may include pedestal 51, upper cover (not shown) and spy that microfluid immunoassay, which integrates reagent device 5,
Levy marker detection reaction tube 52.Pedestal 51 is by PP plastics integrated injection molding, it may include is sequentially arranged along reagent flow direction
Washing lotion storage tank 511, marker film loading drain structure 512, reaction pipe mounting seat 513 and liquid waste tank 514.The upper cover with
The shape of pedestal 51 is consistent, and in conjunction with 51 inserted type of pedestal, and to form closed washing lotion storage tank 511, marker film loading is led
Liquid structure 512 and liquid waste tank 514.For the ease of direction of distinguishing one from the other, pedestal 51 is relatively narrow in one end of washing lotion storage tank 511.In addition,
In order to facilitate crawl, the middle section of pedestal 51 is equipped with recess portion 515.
As shown in Fig. 2, the washing lotion storage tank 511 is an oval cavity body structure, it is located at one end of pedestal 51.It washes at this
Liquid storage tank is equipped with opening 5111 towards the side of marker film loading drain structure 512, has a cutting edge shape protrusion in the opening, interior
Set pricker 5112.Sealing washing lotion bag 5113 is placed in washing lotion storage tank 511.In centrifugation, centrifugal force can push away washing lotion bag 5113
To pricker 5112, pricker 5112 punctures washing lotion bag 5113, and washing lotion is flowed out from washing lotion bag 5113, by 5111, marker of being open
Film loading drain structure 512 enters characteristic indication analyte detection reaction tube 52, completes washing function.Washing lotion bag 113, which is provided with, to be enough
The sufficiently washing lotion of washing characteristic indication analyte detection reaction tube 2.In one embodiment, the washing lotion volume in washing lotion bag 5113 is
1mL or so.
With continued reference to Fig. 2, marker film loading drain structure 512 be located at washing lotion storage tank 511 and reaction pipe mounting seat 513 it
Between, it may include marker film fixing card 5121, liquid guiding cavity 5122, Sample introduction mouth 5123 and washing lotion introducing port 5124.Marker
Film fixing card 5121 is used to for marker film 5125 being pushed on the inlet of the inner cavity 521 of characteristic indication analyte detection reaction tube 52
On marker film position in 5211, and then physical structure active force is borrowed to be fixed on marker film position.Liquid guiding cavity 5122 is used for
Make washing lotion and the labeled object film of Sample introduction cocurrent to characteristic indication analyte detection reaction tube 52.That is, liquid guiding cavity 5122 is led with sample
Entrance 5123 and washing lotion introducing port 5124 are in fluid communication.Sample introduction mouth 5123 is that a kind of cross binds out closing structure, sample introduction
Needle imports sample after opening, sample introduction needle exits after-opening closure, and washing lotion when centrifugation is avoided to export.The washing lotion introducing port 5124 with
(specifically, 5111) opening is in fluid communication washing lotion storage tank, and washing lotion borrows high speed centrifugation power to import liquid guiding cavity 5122.
As shown in Fig. 2 to 4d, it is light-permeable that characteristic indication object reaction tube 52 is molded by PS plastic.Characteristic indication
Object reaction tube 52 is embedded in reaction pipe mounting seat 513.Specifically, reaction pipe mounting seat 513 is equipped with edge 5131, feature mark
Will object reaction tube 52 is fixed by edge 5131.Therefore, in centrifugation, characteristic indication object reaction tube 52 will not be moved.Feature mark
Will object reaction tube 52 has slit-shaped inner cavity 521, and this structure is conducive to generate capillarity propulsion reaction process.That is, intracavitary energy
Capillarity is formed, immobilization process and driving detection reaction are conducive to.The cross section of inner cavity 521 can be round, semicircle
(as shown in fig. 4 a), rectangle (as shown in Figure 4 b), oval (as illustrated in fig. 4 c) or trapezoidal (as shown in figure 4d) etc..Inner cavity 521
Wall surface 5213 be reaction surface, the upper surface 522 of characteristic indication object reaction tube 52 is inspection surface.Preferably, the wall surface of inner cavity 521
Equipped with arc or ripple struction, to increase its surface area, i.e. increase reaction surface area, and then raising reaction speed.Feature mark
52 both ends of will object reaction tube are concordantly open, that is, 21 both ends of inner cavity are respectively inlet 5211 and liquid outlet 5212.Inlet 5211
It is in fluid communication with the liquid guiding cavity 5122 of marker film loading drain structure 512, liquid outlet 5212 and 514 fluid of liquid waste tank connect
It is logical.5211 flare of inlet, preferably to load marker film 5125.
In the shown embodiment, characteristic indication object reaction tube 52 is provided with the importing of luminous substrate liquid at the liquid outlet
Hole 523, the luminous substrate liquid entrance hole 523 is for being added luminous substrate liquid.Luminous substrate liquid entrance hole 523 is equipped with sealing
Device (such as sealing film), which is opened when needing to be added luminous substrate liquid.
Characteristic indication object reaction tube 52 may also include protective edge 524, and 524 one side of protective edge is reacted for protection feature marker
Pipe 52, on the other hand for cooperating with the edge of pedestal, with preferably fixed character marker reaction tube 52.
As shown in Fig. 2, liquid waste tank 514 is a flat shape of a hoof, cavity structure closed at one end.Certainly, waste liquid stores up
Slot 514 can also use other configurations.Liquid waste tank 514 is located at the rear end of characteristic indication analyte detection reaction tube 52, interior filling
There is water-absorbent material 5141.Water-absorbent material 5141 is used for absorbing reaction waste and washing lotion, to avoid refluence.Water-absorbent material 5141 can
To be sponge or resin etc..Preferably, liquid waste tank 514 is (specific by the mouth of a gorge 5142 and characteristic indication analyte detection reaction tube 52
Ground, liquid outlet 5212) it is connected, it is flow backwards to avoid unabsorbed reaction waste and washing lotion.
Fig. 1 is returned to, luminous substrate liquid filling apparatus 3 includes luminous substrate liquid bottle 31, luminous substrate fluid catheter 32, shine bottom
Thing liquid dispenser pump 33, the mobile motor 34 of luminous substrate liquid filling needle and luminous substrate liquid fill needle 35.Luminous substrate fluid catheter 32
It connects luminous substrate liquid bottle 31 and luminous substrate liquid fills needle 35, luminous substrate liquid dispenser pump 33 is mounted on luminous substrate fluid catheter
On 32, the mobile motor 34 of luminous substrate liquid filling needle is for moving luminous substrate liquid filling needle 35.When addition luminous substrate liquid
When instruction is transmitted to luminous substrate liquid filling apparatus 3, luminous substrate liquid fills needle mobile motor 34 and luminous substrate liquid is filled needle
The luminous substrate liquid entrance hole 523 for the characteristic indication object reaction tube 52 that 35 alignments need to be loaded, starts luminous substrate liquid dispenser pump
33, luminous substrate liquid filling needle 35 injects the luminous substrate liquid for formulating volume into reaction tube.Working rotary table 1 rotates a work
Position, injects identical luminous substrate liquid to another characteristic indication object reaction tube 52 in an identical manner.And so on, until complete
Portion's characteristic indication object reaction tube 52 completes luminous substrate liquid sample-adding.
Detection unit 4 includes detector 41 and fluorescence excitation light source 42.Detector 41 is for detecting characteristic indication analyte detection
The fluorescence of reactant in reaction tube 52/shine.Fluorescence excitation light source 42 is for exciting in characteristic indication analyte detection reaction tube 52
Reactant fluorescence.In the case where characteristic indication analyte detection reaction tube 52 is added to luminous substrate liquid, do not need using glimmering
Phot-luminescence source 42.Fluorescence/luminous intensity is related to target concentration to be detected.According to fluorescence/luminous signal intensity and target
Object concentration establishes standard curve, then realizes the qualitative and quantitative analysis to sample object object.If glimmering to reaction zone segmentation detection
Light/luminous signal intensity, accumulative fluorescence/luminous intensity represent conversion zone combining target object number；Segmentation calculates, and can speculate
The amount of each section of combination, such as most back segment fluorescence/luminous extremely weak, fully reacting is represented, such as rearmost end fluorescence/luminous still very strong, table
Show reaction not exclusively, still there are many target levels to participate in reaction, need to dilute quantitative.
The following detailed description of the working principle of the invention.
Firstly, each specimen information and each Samples detection index name needed for this detection is arranged, by each Samples detection index pair
The microfluid immunoassay answered integrates reagent device 5 and is located on the reagent position 121 of working rotary table 1, and prescribed volume will be housed
The hematocrit testing tube 6 of whole blood is loaded on the hematocrit testing tube position 122 of working rotary table 1.By a certain sample
Microfluid immunoassay integrates the rotation of reagent device 5 to sample introduction position.
It is placed in sample cell fixed ring 21 secondly, sample to be detected is placed in sample cell 27, the bar code pair on sample cell 27
It should be in the test surface of sample bar code reading record device 23.After sample detection delivering, sample cell rotation mixes driving structure 22 and revolves
Turn to mix one to for several times, static, upper vertical, sample bar code reading records the bar code information on the scanning sample cell of device 23, automatic sampling apparatus 2
Reagent device successively loading is integrated to the corresponding microfluid immunoassay of project to be checked accordingly, until an examined project of sample is complete
Portion's sample-adding finishes, and is washed out sample introduction needle 23, starts to the corresponding microfluid immunoassay collection of Testing index needed for the 2nd sample
At reagent device successively loading, and so on.
Then, the sample of loading to 5125 region of marker film dissolves marker film pricker 5112, and pricker 5112 will
The marker that washing lotion bag 5113 punctures, and the compound for forming the object in sample with marker by capillary force
It is transferred in the inner cavity of reaction tube 52, with the solid formation association reaction on reaction surface 5213, forms reaction detection chain.Hair is borrowed in reaction
Spy exerts oneself or centrifugal force effect lasts certain time, until the reaction surface 5213 of reaction tube 52 is all covered with and is held by reaction solution
Continue to the defined time.
Intermittent low-speed centrifugal is set and accelerates reaction process, reactant is made to sufficiently achieve total overall reaction face 5213.Part is anti-
Logistics is answered to be integrated in the liquid waste tank 514 of reagent device 5 to microfluid immunoassay, by the water-absorbent material in liquid waste tank 514
5141 absorb delay.
After the reaction time as defined in completing, start washing process.The information such as time, revolving speed according to control program setting
Spoke disk 12 is driven to rotate the defined time with preset revolving speed.At this point, microfluid immunoassay integrates the washing lotion in reagent device 5
Bag 5113 presses to the pricker 5112 in the integrated reagent device 5 of microfluid immunoassay under the action of the centrifugal force, and pricker 5112 will be washed
Liquid bag 5113 punctures, and washing lotion flows to the marker film that microfluid fluorescence of fluorescence immunoassay integrates reagent device along default fluid path
5125, reaction tube 52 eventually flow to liquid waste tank 514, are absorbed and are detained by the water-absorbent material 5141 in liquid waste tank 514, finally
By marker film 5125,52 washes clean of reaction tube.
Microfluid immunoassay after reaction, washing integrates reagent device 5 and successively rotates by preset sequence to luminous bottom
Thing liquid fills station B.When the instruction for adding luminous substrate liquid is transmitted to luminous substrate liquid filling apparatus 3, luminous substrate liquid adds
The luminous substrate for the characteristic indication object reaction tube 52 that the filling alignment of needle 35 of luminous substrate liquid is needed to be loaded by the mobile motor 34 of note needle
Liquid entrance hole 523 starts luminous substrate liquid dispenser pump 33, and luminous substrate liquid filling needle 35 is injected into reaction tube formulates volume
Luminous substrate liquid.Working rotary table 1 rotates a station, injects phase in an identical manner to another characteristic indication object reaction tube 52
Same luminous substrate liquid.And so on, until whole characteristic indication object reaction tubes 52 complete luminous substrate liquid sample-adding.It should refer to
Out, when carrying out fluorescence detection, addition luminous substrate liquid is not needed, therefore, above-mentioned addition luminous substrate liquid omits.
Microfluid immunoassay after reaction, washing or after addition luminous substrate liquid integrates reagent device and presses preset sequence
It successively rotates to detection station C.In the case where fluorescence detection, the fluorescence/detection unit that shines fluorescence excitation light source 42 is excited
The reaction surface 5213 of characteristic indication object reaction tube 52, generates fluorescence, and detector is segmented reaction surface 5213 by mode from the near to the distant
Scanning measures each section of fluorescence/luminous value, calculates each section of fluorescence/luminous value and and each section of fluorescence/luminous value accounting.And it is adding
In the case where luminous substrate liquid (that is, the detection that shines), without exciting fluorescence excitation light source 42, PMT directly detects luminous value.
Fluorescence/shining is completed, high speed rotation 3 minutes again of working rotary table 1 will be complete in hematocrit testing tube 6
Blood continues centrifugation, and static rear hematocrit scale reading device 7 measures blood plasma and haemocyte in hematocrit testing tube
Interior accounts for height, and calculates each sample hematocrit, for calculating target concentration in blood plasma and whole blood.According to having been established and store up
The standard curve deposited calculates the integrated reagent device of each microfluid immunoassay and represents the concentration value that index corresponds to whole blood sample, then
According to hematocrit than conversion plasma concentration value.
Although specifically showing and describing the present invention in conjunction with preferred embodiment, those skilled in the art should be bright
It is white, it is not departing from the spirit and scope of the present invention defined by the appended claims, it in the form and details can be right
The present invention makes a variety of changes, and is protection scope of the present invention.
Claims (10)
1. a kind of microfluid immunoassay integrates reagent automatic detection device, it is characterised in that: including working rotary table, automatic sampling
Device, luminous substrate liquid filling apparatus, hematocrit scale reading device and fluorescence/shine detection unit, the working rotary table
Be divided into even number reagent position, on each reagent position can one microfluid immunoassay of fixed placement integrate reagent device, institute
It states and is symmetrically arranged with a plurality of hematocrit test pipe jigs on working rotary table, the hematocrit test pipe jig is arranged to
Can make to be placed on hematocrit testing tube therein when the working rotary table is rotated from upright position become horizontal position with
The centrifugation of whole blood haemocyte is completed, the automatic sampling apparatus is arranged in by sample introduction station, for blood sample to be detected to be added
It is integrated in reagent device to the microfluid immunoassay for going to the sample introduction station, blood sample to be detected is exempted from the microfluid
Epidemic disease, which is analyzed in integrated reagent device, is reacted and is washed, and the luminous substrate liquid filling apparatus is arranged in the filling of luminous substrate liquid
By station, for luminous substrate liquid to be filled into the characteristic indication object reaction tube for going to the luminous substrate liquid filling station
In, the hematocrit scale reading device is arranged on the fixation position outside the working rotary table, for blood after reading centrifugation
Hematocrit number in specific volume of cell testing tube, the fluorescence/detection unit that shines is arranged in detection station, for going to
The microfluid immunoassay of the detection station integrates the sample after reaction and washing in reagent device and carries out fluorescence/hair
Light detection.
2. microfluid immunoassay as described in claim 1 integrates reagent automatic detection device, it is characterised in that: the miniflow
It includes pedestal, upper cover and characteristic indication analyte detection reaction tube that body immunoassay, which integrates reagent device, along reagent flow on the pedestal
Dynamic direction is successively arranged washing lotion storage tank, marker film loading drain structure, reaction pipe mounting seat and liquid waste tank, the upper cover with
The pedestal inserted type combines, and the characteristic indication analyte detection reaction tube is molded by PS plastic, and fixation is embedded in described anti-
It answers in pipe mounting seat and observation is face-up, and there is slit-shaped inner cavity, promote reaction process to generate capillarity, it is described interior
The both ends of chamber are respectively inlet and liquid outlet, and marker film is fixedly mounted on described by the marker film loading drain structure
In inlet and with liquid guiding cavity and the washing lotion introducing port and Sample introduction mouth that be connected tos with the liquid guiding cavity, the washing lotion storage tank leads to
It crosses the washing lotion introducing port and the liquid guiding cavity is in fluid communication, the liquid guiding cavity and the inlet are in fluid communication, the waste liquid
Storage tank and the liquid outlet are in fluid communication, and it is filled with water-absorbent materials.
3. microfluid immunoassay as claimed in claim 2 integrates reagent automatic detection device, it is characterised in that: the washing lotion
Storage tank is an oval cavity body structure, is inside placed with sealing washing lotion bag, and towards the marker film loading drain structure
Side is equipped with opening, and the opening and the washing lotion introducing port are in fluid communication and are built-in with pricker, and the pricker is washed for puncturing
Liquid bag.
4. microfluid immunoassay as claimed in claim 2 integrates reagent automatic detection device, it is characterised in that: the sample
Introducing port is that cross binds out closing structure, and sample introduction needle imports sample after opening, and sample introduction needle exits after-opening closure.
5. microfluid immunoassay as claimed in claim 2 integrates reagent automatic detection device, it is characterised in that: the waste liquid
Storage tank is in fluid communication by a mouth of a gorge and the liquid outlet.
6. microfluid immunoassay as claimed in claim 2 integrates reagent automatic detection device, it is characterised in that: the feature
Marker reaction tube is provided with luminous substrate liquid entrance hole at the liquid outlet, and the luminous substrate liquid entrance hole is for adding
Enter luminous substrate liquid.
7. microfluid immunoassay as claimed in claim 6 integrates reagent automatic detection device, it is characterised in that: described to shine
Substrate solution filling apparatus includes luminous substrate liquid bottle, luminous substrate fluid catheter, luminous substrate liquid dispenser pump, the filling of luminous substrate liquid
The mobile motor of needle and luminous substrate liquid fill needle, and the luminous substrate fluid catheter connects the luminous substrate liquid bottle and described shines
Substrate solution fills needle, and the luminous substrate liquid dispenser pump is mounted on the luminous substrate fluid catheter, and the luminous substrate liquid adds
The mobile motor of note needle is for moving the luminous substrate liquid filling needle.
8. microfluid immunoassay as described in claim 1 integrates reagent automatic detection device, it is characterised in that: described automatic
Sampling device includes sample cell fixed ring, sample cell rotation mixes driving structure, sample bar code reading records device, sample introduction needle, sample introduction needle
Support frame and sample introduction needle actuating device, the sample cell fixed ring be mounted on sample cell rotation mix in driving structure and by
It drives rotation the blood sample in the sample cell being fixed in the sample cell fixed ring to be uniformly mixed, the sample bar code reading
Record device is used to read bar code and upload on the sample cell, and the sample introduction needle is mounted on the sample introduction needle rack, it is described into
Sample needle actuating device drives the sample introduction needle to sampling position, sample-adding position and washing position for driving the sample introduction needle rack,
And then the sample introduction needle is made to implement sampling, sample-adding and wash certainly.
9. microfluid immunoassay as claimed in claim 2 integrates reagent automatic detection device, it is characterised in that: the detection
Unit includes detector and excitation light source, in the light projection to the characteristic indication analyte detection reaction tube of the excitation light source transmitting
Fluorescence is issued with provocative reaction object, the detector is used to detect the glimmering of the reactant in the characteristic indication analyte detection reaction tube
Light/shine.
10. microfluid immunoassay as described in claim 1 integrates reagent automatic detection device, it is characterised in that: the blood
Specific volume of cell, which tests pipe jig, has C-shaped cross-section, and upper surface is equipped with card slot, the nozzle of the hematocrit testing tube
Place is equipped with the cylindrical projections of a pair of of radial symmetric, and the cylindrical projections are rotationally connected in the card slot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910217642.XA CN109975564B (en) | 2019-03-21 | 2019-03-21 | Automatic detection device for microfluid immunoassay integrated reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910217642.XA CN109975564B (en) | 2019-03-21 | 2019-03-21 | Automatic detection device for microfluid immunoassay integrated reagent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109975564A true CN109975564A (en) | 2019-07-05 |
| CN109975564B CN109975564B (en) | 2022-12-13 |
Family
ID=67079986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910217642.XA Active CN109975564B (en) | 2019-03-21 | 2019-03-21 | Automatic detection device for microfluid immunoassay integrated reagent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109975564B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110967307A (en) * | 2019-11-22 | 2020-04-07 | 宁波紫园医疗器械有限公司 | Hematocrit measuring device |
| CN113281499A (en) * | 2021-05-18 | 2021-08-20 | 厦门先明生物技术有限公司 | Microfluidic immunoassay joint detection device and using method thereof |
| CN113419073A (en) * | 2020-03-30 | 2021-09-21 | 厦门先明生物技术有限公司 | High-flux full-automatic micro-fluidic fluorescence immunoassay device |
| CN117871853A (en) * | 2024-03-13 | 2024-04-12 | 南通戴尔诺斯生物科技有限公司 | Colloidal gold immunochromatography equipment for detecting biotoxin and application method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102680441A (en) * | 2011-03-16 | 2012-09-19 | 罗姆股份有限公司 | Analysis chip, analysis system, and analysis method |
| CN104698199A (en) * | 2015-04-01 | 2015-06-10 | 山东美医林电子仪器有限公司 | Hemorheology detecting device |
| CN107102131A (en) * | 2017-07-03 | 2017-08-29 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system and its detection method |
| CN107407662A (en) * | 2015-03-13 | 2017-11-28 | 生物辐射实验室股份有限公司 | Test case |
| CN109061207A (en) * | 2018-06-21 | 2018-12-21 | 微粒云科技（北京）有限公司 | A kind of biochip for heart infarction marker detection, detection method |
-
2019
- 2019-03-21 CN CN201910217642.XA patent/CN109975564B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102680441A (en) * | 2011-03-16 | 2012-09-19 | 罗姆股份有限公司 | Analysis chip, analysis system, and analysis method |
| CN107407662A (en) * | 2015-03-13 | 2017-11-28 | 生物辐射实验室股份有限公司 | Test case |
| CN104698199A (en) * | 2015-04-01 | 2015-06-10 | 山东美医林电子仪器有限公司 | Hemorheology detecting device |
| CN107102131A (en) * | 2017-07-03 | 2017-08-29 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system and its detection method |
| CN109061207A (en) * | 2018-06-21 | 2018-12-21 | 微粒云科技（北京）有限公司 | A kind of biochip for heart infarction marker detection, detection method |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110967307A (en) * | 2019-11-22 | 2020-04-07 | 宁波紫园医疗器械有限公司 | Hematocrit measuring device |
| CN113419073A (en) * | 2020-03-30 | 2021-09-21 | 厦门先明生物技术有限公司 | High-flux full-automatic micro-fluidic fluorescence immunoassay device |
| CN113419073B (en) * | 2020-03-30 | 2024-05-31 | 厦门先明生物技术有限公司 | High-flux full-automatic microfluidic fluorescence immunoassay device |
| CN113281499A (en) * | 2021-05-18 | 2021-08-20 | 厦门先明生物技术有限公司 | Microfluidic immunoassay joint detection device and using method thereof |
| CN117871853A (en) * | 2024-03-13 | 2024-04-12 | 南通戴尔诺斯生物科技有限公司 | Colloidal gold immunochromatography equipment for detecting biotoxin and application method thereof |
| CN117871853B (en) * | 2024-03-13 | 2024-05-24 | 南通戴尔诺斯生物科技有限公司 | Colloidal gold immunochromatography equipment for detecting biotoxin and application method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109975564B (en) | 2022-12-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109975564A (en) | A kind of integrated reagent automatic detection device of microfluid immunoassay | |
| US11268970B2 (en) | Automatic analysis device and sample analysis method | |
| US11162962B2 (en) | Automatic analysis device and sample analysis method | |
| CN109142768B (en) | Automatic analyzer and sample analyzing method | |
| Schembri et al. | Centrifugation and capillarity integrated into a multiple analyte whole blood analyser | |
| CN109030813A (en) | A kind of chemiluminescence immunoassay detection micro-fluidic chip, detector and detection method | |
| US6299839B1 (en) | System and methods for performing rotor assays | |
| US5827746A (en) | Method to determine the sedimentation of blood and relative device | |
| CN103063856B (en) | Full-automatic fecal occult blood analyzer | |
| CN109975563A (en) | A kind of integrated reagent device of microfluid immunoassay | |
| CN116840499A (en) | Automatic analysis device and sample analysis method thereof | |
| CN108663303A (en) | A kind of blood analyser | |
| CN109030812A (en) | A kind of micro-fluidic chip based on immune detection and biochemistry detection, detector and detection method | |
| CN205138989U (en) | Biochemical immunity mixes analytical equipment | |
| TW201425573A (en) | Testing microfluid system with a multiple-channel disc and utility thereof | |
| CN208607232U (en) | A kind of chemiluminescence immunoassay detection micro-fluidic chip and detector | |
| CN107843583A (en) | A kind of micro-fluidic chemiluminescent analyzer and application method | |
| CN107966578A (en) | A kind of medical quick biochemical detection system and detection method | |
| CN207380068U (en) | A kind of micro-fluidic chemiluminescent analyzer | |
| US20230131712A1 (en) | Blood volume measurement with fluorescent dye | |
| CN111579766B (en) | Disc-type microfluidic fluorescence immunoassay analyzer and immunoassay item testing method | |
| CN208607231U (en) | A kind of micro-fluidic chip and detector based on immune detection and biochemistry detection | |
| CN110231472A (en) | A kind of chemical illumination immunity analysis instrument | |
| CN109142705B (en) | Biochemical immunity analyzer and working method thereof | |
| CN207036854U (en) | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |