CN109952378B - 经修饰的寡核苷酸及使用方法 - Google Patents

经修饰的寡核苷酸及使用方法 Download PDF

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CN109952378B
CN109952378B CN201780070383.1A CN201780070383A CN109952378B CN 109952378 B CN109952378 B CN 109952378B CN 201780070383 A CN201780070383 A CN 201780070383A CN 109952378 B CN109952378 B CN 109952378B
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oligonucleotide
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nucleotides
hbv
modified
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CN109952378A (zh
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S.格亚兹诺夫
L.比尔格曼
J.洪
V.拉万施
S.马蒂内.蒙特罗
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Jensen Biopharmaceutical Co ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/712—Nucleic acids or oligonucleotides having modified sugars, i.e. other than ribose or 2'-deoxyribose
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7125—Nucleic acids or oligonucleotides having modified internucleoside linkage, i.e. other than 3'-5' phosphodiesters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A61K47/549—Sugars, nucleosides, nucleotides or nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
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    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/11—Antisense
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
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    • C12N2310/314—Phosphoramidates
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    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/314—Phosphoramidates
    • C12N2310/3145—Phosphoramidates with the nitrogen in 3' or 5'-position
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/32—Chemical structure of the sugar
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    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
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    • C12N2310/30—Chemical structure
    • C12N2310/34—Spatial arrangement of the modifications
    • C12N2310/341—Gapmers, i.e. of the type ===---===
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    • C12N2310/30—Chemical structure
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    • C12N2310/3525—MOE, methoxyethoxy
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  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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CN201780070383.1A 2016-09-14 2017-09-14 经修饰的寡核苷酸及使用方法 Active CN109952378B (zh)

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US201662394738P 2016-09-14 2016-09-14
US201662394739P 2016-09-14 2016-09-14
US62/394739 2016-09-14
US62/394738 2016-09-14
PCT/US2017/051644 WO2018053185A1 (en) 2016-09-14 2017-09-14 Modified oligonucleotides and methods of use

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CN109952378B true CN109952378B (zh) 2024-09-17

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EP (1) EP3512949A1 (https=)
JP (3) JP6970187B2 (https=)
KR (3) KR20240010761A (https=)
CN (1) CN109952378B (https=)
AU (2) AU2017326372B2 (https=)
BR (1) BR112019004911A2 (https=)
CA (1) CA3037042A1 (https=)
IL (2) IL265359B2 (https=)
MA (1) MA46422A (https=)
MX (2) MX2019002960A (https=)
TW (1) TWI769177B (https=)
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CA3187388A1 (en) * 2020-07-27 2022-02-03 Jin Hong Hbv binding oligonucleotides and methods of use
CN114057816B (zh) * 2020-07-30 2025-02-11 浙江柏拉阿图医药科技有限公司 富含腺嘌呤硫代磷酸酯化寡核苷酸及其抗肝炎病毒的应用
WO2022153358A1 (ja) 2021-01-12 2022-07-21 日本電気株式会社 秘密計算システム、秘密計算サーバ装置、秘密計算方法および秘密計算プログラム
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US20210040483A1 (en) 2021-02-11
JP7478210B2 (ja) 2024-05-02
JP2023011793A (ja) 2023-01-24
KR20240010761A (ko) 2024-01-24
AU2017326372B2 (en) 2023-12-07
JP7170820B2 (ja) 2022-11-14
TWI769177B (zh) 2022-07-01
JP2019529406A (ja) 2019-10-17
TW201825103A (zh) 2018-07-16
JP2022023936A (ja) 2022-02-08
KR102533038B1 (ko) 2023-05-17
MA46422A (fr) 2019-07-24
KR20230033736A (ko) 2023-03-08
AU2024201485A1 (en) 2024-03-28
IL265359B1 (en) 2024-10-01
CA3037042A1 (en) 2018-03-22
IL265359A (en) 2019-05-30
RU2019111020A3 (https=) 2021-06-22
KR102627418B1 (ko) 2024-01-19
US20250092400A1 (en) 2025-03-20
MX2019002960A (es) 2019-09-18
US20180135054A1 (en) 2018-05-17
IL265359B2 (en) 2025-02-01
AU2017326372A1 (en) 2019-04-04
MX2023014145A (es) 2023-12-13
US12077757B2 (en) 2024-09-03
JP6970187B2 (ja) 2021-11-24
RU2019111020A (ru) 2020-10-15
WO2018053185A1 (en) 2018-03-22
US10793859B2 (en) 2020-10-06
IL315071A (en) 2024-10-01
BR112019004911A2 (pt) 2019-06-25
CN109952378A (zh) 2019-06-28
EP3512949A1 (en) 2019-07-24
KR20190047025A (ko) 2019-05-07

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