CN109937869A - A kind of regulation method of sargassum fusifome juvenile sporophyte growth - Google Patents
A kind of regulation method of sargassum fusifome juvenile sporophyte growth Download PDFInfo
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- CN109937869A CN109937869A CN201910180231.8A CN201910180231A CN109937869A CN 109937869 A CN109937869 A CN 109937869A CN 201910180231 A CN201910180231 A CN 201910180231A CN 109937869 A CN109937869 A CN 109937869A
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Abstract
The invention discloses a kind of regulation methods of sargassum fusifome juvenile sporophyte growth.This method comprises the following steps: by sargassum fusifome gamogenesis support co-incubation between the 5-7 month, collecting zygote；Use seawater as culture environment culture zygote；Inorganic nitrogen salt and Inorganic phosphate are added into culture vessel；Uninterruptedly it is pumped into air agitation seawater, by the content for adding inorganic nitrogen salt and Inorganic phosphate in rich seawater, regulate and control the growth length of sargassum fusifome juvenile sporophyte frond, enhances the photosynthesis of sargassum fusifome juvenile sporophyte, so that sargassum fusifome juvenile sporophyte frond is more sturdy solid.Method provided by the invention adds inorganic nitrogen salt and Inorganic phosphate into culture seawater, suitable ecological environmental condition is provided for sargassum fusifome sexual propagation, survival and growth and development to sargassum fusifome fertilized eggs and juvenile sporophyte carry out artificial regulatory, promote frond synthetic dyestuff, accumulation nutriment, promote the growth and development of sargassum fusifome juvenile sporophyte, and then improves nursery success rate.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of regulation method of sargassum fusifome juvenile sporophyte growth.
Background technique
Sargassum fusifome [Hizikia fusiformis (Harvey) Okamura (Sargassaceae, Phaeophyta)] belongs to
Phaeophyta, dumpling made of glutinous rice flour guiding principle, Fucales, Sargassaceae, Sargassum usually divide sargassum fusifome into Japan new in Sargassaceae
Belong to Hizikia.Sargassum fusifome is the perennial seaweed in warm temperate zone, be the peculiar type of North Western Pacific, be distributed in South Korea, Japan and I
On the low tide band rock reef of state bank, extensively it is distributed in northern China and south, especially more with Zhejiang, Fujian and Coast of Guangdong Province resource
It is abundant.
Sargassum fusifome dioecism spends winter by remaining rhizoid, and spring next year is by rhizoid regeneration plant.Annual reproduction
Season, gamogenesis support are generated from base of leaf, and gamete (ovum and sperm) is discharged respectively from the genital fossa in gamogenesis support.
Sargassum fusifome gamete release be one dependent on photosynthesis and related zwitterion transfer physiology course, be illuminated by the light, temperature,
The influence of the environmental factors such as seawater salinity, seawater movement.After fertilization fertilized eggs are sprouted into juvenile sporophyte, and formation rhizoid is grown in attached
On base.The phenology of sargassum fusifome sexual propagation is related with the latitude (periodicity of illumination and ocean temperature) of its growth distribution, and has
There are dependences with sargassum fusifome individual plants size again for sexual reproduction strategy.Phase, the height being increasingly enhanced are trained in sargassum fusifome sexual propagation
Warm condition causes violent negative effect to receptacle metabolic activity, with training, high temperature strong inhibition receptacle gamete release and matches
Sub- vitality.
Sargassum fusifome has very big economy and Development volue, and demand of the domestic and international market to sargassum fusifome is very big, therefore, China
The especially sea areas such as Zhejiang, Guangdong cultivation that strengthens sargassum fusifome seaweed.Seedling is the key that sargassum fusifome expands cultivation, but sheep dwells
The seedling problem of dish still annoyings the development of sargassum fusifome aquaculture so far.There are mainly two types of sides for the seeling industry of sargassum fusifome at present
Seedling is trained first is that relying on rhizoid regeneration and carrying out rhizoid, this mode needs a large amount of wild or cultivation frond likes:；Another kind is benefit
The method for collecting fertilized eggs or juvenile sporophyte with zoogamy mode, but the success rate of the method nursery at present is also very low, is producing
The contribution of seed is less than 10%.
Summary of the invention
For overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of regulations of sargassum fusifome juvenile sporophyte growth
Method.
The purpose of the present invention is realized at least through one of following technical solution.
In order to improve the breeding efficiency of sargassum fusifome, the present invention supplies condition by the nutritive salt in control culture seawater, is
Sargassum fusifome sexual propagation provides suitable ecological environmental condition, survival and growth and development to sargassum fusifome fertilized eggs and juvenile sporophyte
Artificial regulatory is carried out, nursery success rate is improved.
The present invention provides a kind of regulation method of sargassum fusifome juvenile sporophyte growth, includes the following steps: that handle is attached to fabric
Sargassum fusifome juvenile sporophyte moves on to culture pond (culture vessel) on item, and culture environment is nature seawater, wherein nature seawater it is inorganic
Pyridine floors is 10-20 μM, and Phos background values is 0.5-1.5 μM；It is a micro- that 100-200 is additionally added into every liter of culture seawater
Mole inorganic nitrogen salt NO3 -(NaNO3) and 15-60 micromolar Inorganic phosphate PO4 3-(NaH2PO4), promote frond synthesis
Pigment, accumulation nutriment and growth and development.
A kind of sargassum fusifome juvenile sporophyte growth provided by the invention regulation method (by control nutritive salt supply condition come
Regulate and control the growth of sargassum fusifome juvenile sporophyte), include the following steps:
(1) the sargassum fusifome gamogenesis support co-incubation to cut one's eye-teeth substantially, sterilizing yarn is put into bottom of culture vessel
Cloth (layer 2-3, preferably 2 layers of sterile gauze), convenient for the attachment and collection of zygote；
(2) zygote that incubation step (1) is collected is grown to sargassum fusifome juvenile sporophyte；
(3) step (2) the sargassum fusifome juvenile sporophyte is transferred in culture vessel, using nature seawater as culture environment；
(4) inorganic nitrogen salt and Inorganic phosphate are added into culture vessel, is uninterruptedly pumped into air agitation seawater and (pumps from the external world
It is pumped into the air of circulation good area, the daily 24 small continuous agitation seawater of training), it is pumped into air agitation cultivation in sea water；
(5) nature seawater is replaced, inorganic nitrogen salt and Inorganic phosphate is added again, is pumped into air agitation seawater, continues to cultivate,
By adding the content of inorganic nitrogen salt and Inorganic phosphate in rich seawater, regulates and controls the growth length of sargassum fusifome juvenile sporophyte, enhance sheep
It dwells the photosynthesis of dish juvenile sporophyte, so that sargassum fusifome juvenile sporophyte frond is sturdy solid.
Further, the training phase of sargassum fusifome gamogenesis support co-incubation described in step (1) is sargassum fusifome sexual propagation
The training phase, i.e., the annual 5-7 month.
Under conditions of longer periodicity of illumination (in the Dark-light cycle of 24 small trainings, preferably the photoperiod is not less than 12 small trainings)
Biggish egg cell emission index and zygote generation rate can be generated.From the female receptacle material of 100kg, can about it obtain
5.0 hundred million zygotes；Zygote is attached on the fabric strip with carpet hair (cloth), reaches 2.5-3.5mm or so training to its length,
Sargassum fusifome juvenile sporophyte is obtained, and it is moved on into culture vessel；
Further, the length of sargassum fusifome juvenile sporophyte described in step (2) is 2.5-3.5mm.
Further, step (3) and the background values of inorganic nitrogen in nature seawater described in step (5) are 10-20 μm of ol/L,
The background values of Phos is 0.5-1.5 μm of ol/L in nature seawater described in step (3) and step (5).
Further, step (4) and inorganic nitrogen salt described in step (5) are NaNO3；Described in step (4) and step (5)
Inorganic phosphate is NaH2PO4。
Further, the additive amount of step (4) and inorganic nitrogen salt described in step (5) rubs for every liter of seawater 100-200 is micro-
You；The additive amount of Inorganic phosphate described in step (4) and step (5) is every liter of seawater 15-60 micromole.The addition of inorganic salts can
Promote frond synthetic dyestuff, accumulation nutriment and growth and development.
Further, the rate that air agitation cultivation in sea water is pumped into described in step (4) is 400-600mL/min, step
(4) described to be pumped between the training of air agitation cultivation in sea water as 36-60h.
Further, the rate that air agitation seawater is pumped into described in step (5) is 400-600mL/min, step (5) institute
State between the training for being pumped into air agitation seawater is 30-35 days.
Further, step (5) the replacement nature seawater is that every 36-60h replacement is primary；Every replacement is primary naturally extra large
Water need to add inorganic nitrogen salt and Inorganic phosphate again.
Further, it is 30-35 days between step (5) training for continuing culture, continues to need every 36-60h more during culture
A nature seawater is changed, needs to add inorganic nitrogen salt and Inorganic phosphate again after replacement, the two additive amount is identical as step (3)；
Replacement nature seawater is to terminate training replacement in step (4) the air agitation cultivation in sea water that is pumped into for the first time, then every 36-60h
Replace a nature seawater.
Compared with prior art, the invention has the advantages that and the utility model has the advantages that
Regulation method provided by the invention regulates and controls sargassum fusifome children spore by adding appropriate inorganic nitrogen and inorganic phosphorus recycling
The growth rate of daughter enhances its photosynthesis and accumulates the ability of nutriment, so that sargassum fusifome juvenile sporophyte be made to grow
It is sturdy solid, promote the synthesis of chlorophyll a, chlorophyll b and carotenoid, improve photosynthetic rate, makes sargassum fusifome metabolism development
Well, nursery success rate is improved.
Detailed description of the invention
Fig. 1 is the growth length folding that sargassum fusifome juvenile sporophyte supplies under treatment conditions in different N, P in the embodiment of the present invention
Line chart；
Fig. 2 a is photosynthetic rate column of the sargassum fusifome juvenile sporophyte under different N, P supply treatment conditions in the embodiment of the present invention
Shape figure；
Fig. 2 b is respiratory rate column of the sargassum fusifome juvenile sporophyte under different N, P supply treatment conditions in the embodiment of the present invention
Shape figure；
Fig. 2 c be the embodiment of the present invention in sargassum fusifome juvenile sporophyte different N, P supply treatment conditions under respiratory rate with
The ratio bar graphs of gross photosynthesis rate；
Fig. 3 a is that sargassum fusifome juvenile sporophyte contains in the chlorophyll a that different N, P are supplied under treatment conditions in the embodiment of the present invention
Measure histogram；
Fig. 3 b is that sargassum fusifome juvenile sporophyte contains in the chlorophyll b that different N, P are supplied under treatment conditions in the embodiment of the present invention
Measure histogram；
Fig. 3 c is carotenoid of the sargassum fusifome juvenile sporophyte under different N, P supply treatment conditions in the embodiment of the present invention
Content histogram.
Specific embodiment
Below in conjunction with embodiment, the present invention will be described in detail so that advantages and features of the invention can be easier to by
It will be appreciated by those skilled in the art that embodiments of the present invention are not limited thereto.If it is noted that having below not especially specifically
Bright process or parameter is that those skilled in the art can refer to prior art understanding or realize.
The acquisition and pre-treatment of the sargassum fusifome young
(1) acquisition of juvenile sporophyte: June is the sargassum fusifome sexual propagation training phase of Shantou Nan ' ao Island growth, mature sheep
It dwells dish gamogenesis support co-incubation, 2 layers of sterile gauze is put into container bottom, convenient for the attachment and collection of zygote.Zygote is attached
On the fabric strip (cloth) with carpet hair.
(2) laboratory of juvenile sporophyte is temporarily supported: zygote described in step (1) being carried out preculture in laboratory, makes its hair
It educates for sargassum fusifome juvenile sporophyte.The condition of preculture is that temperature is 20 DEG C, and light intensity is 70 μm of ol m-2s-1, photoperiod 12h:
12h；Culture solution be filtering nature seawater (nutritive salt background values concentration: inorganic nitrogen concentration be 20 μM, inorganic phosphorus concentration be 1.0 μ
M), every two days replacement nature seawaters are primary, carry out air vent (rate 400mL/min) culture for 24 hours daily.
(3) experiment process: the cloth for being attached with uniform size, healthy growth, the sargassum fusifome juvenile sporophyte being evenly distributed is taken
10 (every cloth size 20mm × 20mm, the juvenile sporophyte with 20 plants or so).When every plant of juvenile sporophyte length reaches 2.5-
3.5mm training, carries out adjusting and controlling growth according to the method provided by the invention.
Embodiment 1
(1) respectively by be attached with uniform size, healthy growth, the sargassum fusifome juvenile sporophyte being evenly distributed square cloth
10 (every cloth size is 20mm × 20mm, and with 20 plants or so of juvenile sporophyte, the initial length of every plant of juvenile sporophyte is
2.5-3.5mm), it is put into the 5000mL conical flask of two installation 5L nature seawaters (each conical flask is put into 10 cloths)
In, the NO in the nature seawater (control seawater)3 -Concentration is 10 μm of olL-1, PO4 3-Concentration is 0.5 μm of olL-1, herein
On the basis of to two conical flasks add inorganic nitrogen salts, do not add microcosmic salt.The additive amount difference of two conical flask inorganic nitrogen salts
For every liter of 0 micromole of seawater and 200 micromoles, it is respectively labeled as N-P0 and N+P0, two groups are compared.
(2) by air pump from extraneous toward being pumped into air in the conical flask for cultivating sargassum fusifome juvenile sporophyte to stir seawater,
Rate is 400mL/min, continuously stirs seawater, cultivates sargassum fusifome juvenile sporophyte, is 36h between culture training；Temperature, the light intensity of culture
It is identical that condition is temporarily supported with laboratory with photoperiod etc..
(3) nature seawater is replaced after the continuous agitation cultivation in sea water of step (2), gives N-P0 and N+P0 two respectively
Group adds inorganic nitrogen salt again, and two groups are not added microcosmic salt, and N-P0 and two groups of N+P0 of inorganic nitrogen salt additive amount are respectively every liter
0 micromole of seawater and 200 micromoles.
(4) continue culture 35 days, seawater (nature seawater) is cultivated in every 36h replacement, after replacing every time again by above-mentioned requirements
It adds inorganic nitrogen salt (additive amount of two groups of inorganic nitrogen salts is identical with step (3)).Temperature, light intensity and photoperiod of culture etc.
It is identical that condition is temporarily supported with laboratory.By regulating and controlling the growth length of sargassum fusifome juvenile sporophyte, enhancing sargassum fusifome juvenile sporophyte
Photosynthesis, so that sargassum fusifome juvenile sporophyte frond is sturdy solid.
Fig. 1 is growth length line chart of the sargassum fusifome juvenile sporophyte under different N, P supply treatment conditions, from fig. 1, it can be seen that
The ratio of relative growing rate N+P0 of the sargassum fusifome juvenile sporophyte under N-P0 processing is higher, and frond is more slender very thin；And in N
The lower growth rate of+P0 processing is slightly slow but more sturdy solid, and has higher photosynthetic rate (see Fig. 2 a), chlorophyll a, Ye Lv
Plain b and carotenoid content also enrich (see Fig. 3 a, Fig. 3 b, Fig. 3 c) than N-P0.
Embodiment 2
(1) respectively by be attached with uniform size, healthy growth, the sargassum fusifome juvenile sporophyte being evenly distributed square cloth
10 (every cloth size is 20mm × 20mm, and with 20 plants or so of juvenile sporophyte, the initial length of every plant of juvenile sporophyte is
2.5-3.5mm), it is put into the 5000mL conical flask of two installation 5L nature seawaters (each conical flask is put into 10 cloths)
In, the NO in the nature seawater (control seawater)3 -Concentration is 20 μm of olL-1, PO4 3-Concentration is 1 μm of olL-1, in this base
Respectively to two conical flask addition inorganic nitrogen salts and Inorganic phosphate on plinth.The additive amount difference of two conical flask inorganic nitrogen salts
For every liter of micromole of seawater 0 and 200, the additive amount of two conical flask Inorganic phosphates is 15 micromole of every liter of seawater, respectively
Labeled as N-P15 and N+P15, two groups are compared.
(2) by air pump from extraneous toward being pumped into air in the conical flask for cultivating sargassum fusifome juvenile sporophyte to stir seawater,
Rate is 500mL/min, continuously stirs seawater, cultivates sargassum fusifome juvenile sporophyte, is 48h between culture training；Temperature, the light intensity of culture
It is identical that condition is temporarily supported with laboratory with photoperiod etc..
(3) a nature seawater continuously is replaced after agitation cultivation in sea water, respectively again to N-P15 and two groups of N+P15
Inorganic nitrogen salt is added, the additive amount of two conical flask inorganic nitrogen salts is respectively the micromole of every liter of seawater 0 and 200, two triangles
The additive amount of flask Inorganic phosphate is 15 micromole of every liter of seawater.
(4) continue culture 33 days, seawater (nature seawater) is cultivated in every 48h replacement, after replacing every time again by above-mentioned requirements
It adds inorganic nitrogen salt (additive amount of two groups of inorganic nitrogen salts and Inorganic phosphate is identical with step (3)).Temperature, the light intensity of culture
It is identical that condition is temporarily supported with photoperiod equalization with laboratory.By regulating and controlling the growth length of sargassum fusifome juvenile sporophyte, enhance sargassum fusifome
The photosynthesis of juvenile sporophyte, so that sargassum fusifome juvenile sporophyte frond is sturdy solid.
Fig. 1 is growth length line chart of the sargassum fusifome juvenile sporophyte under different N, P supply treatment conditions, from fig. 1, it can be seen that
Sargassum fusifome juvenile sporophyte is low in the ratio of relative growing rate N-P15 that N+P15 is handled, and frond increases relatively slow but thicker in length
Strong, photosynthetic rate is higher, and breathing/gross photosynthesis ratio significantly reduces, and has stronger photosynthetic capacity；And contained chlorophyll a,
Chlorophyll b and carotenoid content also enrich (see Fig. 3 a, Fig. 3 b, Fig. 3 c) than N-P15.
Embodiment 3
(1) respectively by be attached with uniform size, healthy growth, the sargassum fusifome juvenile sporophyte being evenly distributed square cloth
10 (every cloth size is 20mm × 20mm, and with 20 plants or so of juvenile sporophyte, the initial length of every plant of juvenile sporophyte is
2.5-3.5mm), it is put into the 5000mL conical flask of two installation 5L nature seawaters (each conical flask is put into 10 cloths)
In, the NO in the nature seawater (control seawater)3 -Concentration is 16 μm of olL-1, PO4 3-Concentration is 0.8 μm of olL-1, herein
On the basis of to two conical flasks addition inorganic nitrogen salts and Inorganic phosphate.The additive amount of two conical flask inorganic nitrogen salts is respectively
Every liter of micromole of seawater 0 and 200, the additive amount of two conical flask Inorganic phosphates are 60 micromole of every liter of seawater, are marked respectively
It is denoted as N-P60 and N+P60, two groups are compared.
(2) by air pump from extraneous toward being pumped into air in the conical flask for cultivating sargassum fusifome juvenile sporophyte to stir seawater,
Rate is 600mL/min, continuously stirs seawater, cultivates sargassum fusifome juvenile sporophyte, is 60h between culture training；Temperature, the light intensity of culture
It is identical that condition is temporarily supported with laboratory with photoperiod etc..
(3) a nature seawater continuously is replaced after agitation cultivation in sea water, respectively again to N-P60 and two groups of N+P60
Inorganic nitrogen salt is added, the additive amount of two conical flask inorganic nitrogen salts is respectively the micromole of every liter of seawater 0 and 200；It adds inorganic
Microcosmic salt, the additive amount of two conical flask Inorganic phosphates are 60 micromole of every liter of seawater.
(4) continue culture 30 days, seawater (nature seawater) is cultivated in every 60h replacement, after replacing every time again by above-mentioned requirements
Add inorganic nitrogen salt and Inorganic phosphate (additive amount of two groups of inorganic nitrogen salts and Inorganic phosphate is identical with step (3)).Culture
That condition is temporarily supported with laboratory is identical temperature, light intensity and photoperiod etc..By regulating and controlling the growth length of sargassum fusifome juvenile sporophyte,
The photosynthesis for enhancing sargassum fusifome juvenile sporophyte, so that sargassum fusifome juvenile sporophyte frond is sturdy solid.
Fig. 1 is growth length line chart of the sargassum fusifome juvenile sporophyte under different N, P supply treatment conditions, from fig. 1, it can be seen that
The ratio of relative growing rate N-P60 of N+P60 processing is low, and frond increases relatively slow but more sturdy, the light of N+P60 processing in length
Conjunction speed ratio N-P60 is higher, and respiratory rate is close, and breathing/gross photosynthesis ratio is lower, and contained chlorophyll a, chlorophyll b and class are recklessly
Radish cellulose content also enriches (see Fig. 3 a, Fig. 3 b, Fig. 3 c) than N-P60.
Test 1
The relative growth rate (Relative growth rate, RGR) of frond is acquired according to following formula: RGR (%/
D)=100% × (lnNt-lnN0)/t, N in formulatIt is the length (mm) of frond after culture t days, N0For the initial length of frond
(mm), t is between training (unit: day).The length of vernier caliper measurement sargassum fusifome juvenile sporophyte, the sample of every kind of processing are used every other week
20 plants of product random measurement or more, mean value is then taken, as a result as shown in Figure 1.
Test 2
Measure chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoid (Car).The various processing of 0.2g are taken respectively
Frond sample be placed in the methanol of 20mL, be placed in 4 DEG C fridge overnight processing, then use ultraviolet specrophotometer (UV
530, Beckman counlter, USA) 280-700nm wave band to extracting solution carry out light absorption scanning.Chlorophyll a, chlorophyll
The content of b and carotenoid is according to scanning optical spectrum as a result, calculating according to following formula: chlorophyll a=15.65A666-
7.34A653；Chlorophyll b=27.05A653-11.21A666；Carotenoid=(1000A470+1403.57A666-
3473.87A653)/221.Wherein A470、A653、A666It is illustrated respectively in the OD value of 470nm, 653nm and 666nm.
Test 3
Using liquid phase oxygen electrode (YSI Model 5300；Yellow Springs Instrument Co., OH, USA) it surveys
The photosynthetic oxygen evolution (Pn) and Dark respiration rate (Rd) of Dinghai algae.By the constant temperature cooling cycle water-bath of connection (Cole Parmer,
USA the temperature of reactive tank) is made to maintain the preset temperature of experiment, light source is tungsten halogen lamp, by changing between light source and leaf chamber
Distance measures the photosynthesis rate under different illumination intensity, to obtain photosynthetic rate and light intensity to adjust the intensity of light intensity
Relation curve (P-I curve), use light quantum sensor (SKP 200, ELE International, Leighton
Buzzard, UK) measurement intensity of illumination.
Specific test includes the following steps: the fresh frond (Fw) for taking the various processing of 0.1g respectively in the reaction chamber of reactive tank
It is interior, and inject 8mL culture seawater corresponding with embodiment, and (rate is by the stirring of the magnetic stirring apparatus average rate of reactive tank
400rpm) react indoor culture solution (culture seawater) and frond.Seaweed sample passes through 10min under each illumination condition
Adaptation process.The Dark respiration rate (Rd) of frond passes through measurement frond (with black cloth covering reactive tank) under the conditions of complete darkness
Oxygen consumption rate obtain.Micromole's oxygen number that photosynthetic oxygen evolution and Dark respiration rate are increased or consumed per small training with every gram of fresh weight
(μmolO2·g-1Fw·h-1) indicate.The gross photosynthesis rate (Pg) of frond the maximum net photosynthetic speed of action and dark respiration
The adduction of oxygen consumption rate indicates.Each experiment process carries out three parallel laboratory tests.
Interpretation of result:
In three embodiments, sargassum fusifome juvenile sporophyte supplies growing state such as Fig. 1 institute under treatment conditions in different N, P
Show.It can be seen that N adds rich or P that richness is added to decline the growth rate (indicating with length) of sargassum fusifome juvenile sporophyte, growth speed
Rate is maximum to be handled for N-P0, and minimum N+P60 is handled.But compared to nature seawater, under the conditions of N, P add richness (N+P15 and
N+P60), sargassum fusifome juvenile sporophyte individual weight is bigger, and frond is more sturdy solid, and vitality is stronger.
In three embodiments, sargassum fusifome juvenile sporophyte supplies the photosynthetic and respiratory rate under treatment conditions such as in different N, P
Fig. 2 a, Fig. 2 b, shown in Fig. 2 c.It can be seen that inorganic N adds richness to increase the photosynthetic capacity of sargassum fusifome juvenile sporophyte, improve opposite
Electron transport rate；And inorganic P adds richness to have no significant effect photosynthetic capacity in seawater；But N adds rich or P to add both rich in seawater
It can promote the respiration of sargassum fusifome juvenile sporophyte, and breathing/gross photosynthesis ratio is lower.The result shows that N+P15 is with minimum
Breathing/gross photosynthesis ratio, carry out photosynthesis and accumulate nutriment ability it is most strong, and add rich content of inorganic phosphorus higher
N+P60, breathing/gross photosynthesis ratio ratio N+P15 are slightly higher.
In three embodiments, sargassum fusifome juvenile sporophyte supplies pigment content such as Fig. 3 a, figure under treatment conditions in different N, P
Shown in 3b, Fig. 3 c.N increases the chlorophyll a, chlorophyll b and class Hu trailing plants that can significantly improve sargassum fusifome juvenile sporophyte in nature seawater
Foretell cellulose content；Under the conditions of nature seawater N, P adds the rich chlorophyll and carotenoid content for improving sargassum fusifome juvenile sporophyte.
N is given during culture and adds richness, can accumulate more uv-absorbing substances in this seedling body, ultraviolet radioactive is resisted
Ability is also just stronger.It is compared with the frond under normal N level culture, N adds the frond in rich situation to have higher opposing electronic to pass
Rate is passed, it is also more outstanding than the frond under normal N level culture in terms of the damage for resisting high light intensity with training.Therefore in seed
Cultivation production on, it is appropriate increase culture Seawater in N nutrient can improve sargassum fusifome seedling to a certain extent
It can be from room although N adds the rich growth of seedling handled not promoted significantly to the resistivity of environmental stress
The seedling for inside moving on to mariculture area provides the ability for preferably resisting solar ultraviolet radiation.Therefore, above-mentioned the result shows that, this hair
The regulation method of bright offer can regulate and control the growth length of sargassum fusifome juvenile sporophyte, enhance the photosynthetic work of sargassum fusifome juvenile sporophyte
With so that sargassum fusifome juvenile sporophyte frond is sturdy solid.
Above embodiments are only preferrred embodiment of the present invention, for explaining only the invention, are not intended to limit the present invention, this
Field technical staff should belong to guarantor of the invention without departing from change made under spirit of the invention, replacement, modification etc.
Protect range.
Claims (10)
1. a kind of regulation method of sargassum fusifome juvenile sporophyte growth, which comprises the steps of:
(1) sargassum fusifome gamogenesis support co-incubation, it is put into sterile gauze in bottom of culture vessel, collects zygote；
(2) zygote that incubation step (1) is collected is grown to sargassum fusifome juvenile sporophyte；
(3) step (2) the sargassum fusifome juvenile sporophyte is transferred in culture vessel, using nature seawater as culture environment；
(4) inorganic nitrogen salt and Inorganic phosphate are added into culture vessel, is pumped into air agitation cultivation in sea water；
(5) nature seawater is replaced, inorganic nitrogen salt and Inorganic phosphate is added again, is pumped into air agitation seawater, continues to cultivate, is regulated and controled
The growth length of sargassum fusifome juvenile sporophyte enhances the photosynthesis of sargassum fusifome juvenile sporophyte, so that sargassum fusifome juvenile sporophyte frond
It is sturdy solid.
2. regulation method according to claim 1, which is characterized in that sargassum fusifome gamogenesis support described in step (1) is total
Period with culture is the period of sargassum fusifome sexual propagation, i.e., the annual 5-7 month.
3. regulation method according to claim 1, which is characterized in that the length of sargassum fusifome juvenile sporophyte described in step (2)
Degree is 2.5-3.5mm.
4. regulation method according to claim 1, which is characterized in that in nature seawater described in step (3) and step (5)
The background values of inorganic nitrogen is 10-20 μm of ol/L, and step (3) and the background values of Phos in nature seawater described in step (5) are
0.5-1.5μmol/L。
5. regulation method according to claim 1, which is characterized in that step (4) is with inorganic nitrogen salt described in step (5)
NaNO3；Inorganic phosphate described in step (4) and step (5) is NaH2PO4。
6. regulation method according to claim 1, which is characterized in that inorganic nitrogen salt described in step (4) and step (5)
Additive amount is every liter of seawater 100-200 micromole；The additive amount of Inorganic phosphate described in step (4) and step (5) is every liter of sea
Water 15-60 micromole.
7. regulation method according to claim 1, which is characterized in that be pumped into the training of air agitation seawater described in step (4)
Feeding rate is 400-600mL/min, and step (4) time for being pumped into air agitation cultivation in sea water is 36-60h.
8. regulation method according to claim 1, which is characterized in that step is pumped into air agitation seawater described in (5)
Rate is 400-600mL/min, and step (5) time for being pumped into air agitation seawater is 30-35 days.
9. regulation method according to claim 1, which is characterized in that step (5) the replacement nature seawater is every 36-
60h replacement is primary；Nature seawater of every replacement, need to add inorganic nitrogen salt and Inorganic phosphate again；Replacement nature sea for the first time
Water is to replace at the end of being pumped into air agitation cultivation in sea water described in step (4).
10. regulation method according to claim 1, which is characterized in that step (5) time for continuing culture is 30-
35 days, continue that every 36-60h is needed to replace a seawater during culture, seawater of every replacement need to add inorganic nitrogen salt and nothing again
Machine microcosmic salt.
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| CN112806251A (en) * | 2021-02-26 | 2021-05-18 | 温州市洞头区海洋与渔业发展研究中心 | Unit mass evaluation and difference comparison method for cultivating juvenile sporophytes of sargassum fusiforme |
| CN114051921A (en) * | 2021-11-16 | 2022-02-18 | 温州大学 | Method for in vitro preservation of germplasm resources of sargassum fusiforme rhizoid |
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