CN109917137A - 低离子增强液的制备方法 - Google Patents
低离子增强液的制备方法 Download PDFInfo
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Abstract
本发明涉及低离子增强液的制备方法,它包括缓冲液的配制和低离子增强液的配制。本发明低离子增强液制备方法,能够制备出新型增强介质,可以促进红细胞抗体与抗原更快地结合,减少孵育时间,优化加快凝胶法间接抗人球蛋白试验的检测流程。
Description
技术领域
本发明涉及低离子增强液的制备方法。
背景技术
输血发展史上里程碑事件是1900年奥地利维也纳大学科学家KarlLandersteiner等发现了A、B、O血型。两年后,他的学生又发现了AB型血型,从而开创了安全输血的新时代,Landersteiner也因此于1930年获得了诺贝尔医学奖。1927~1947年间相继发现MNS、P和Rh系统,安全的输血技术才得以初步建立。
临床有效用血首先要求临床医生明确哪些患者在何种情况下需要输血,有效治疗凝血障碍和防止输血的不良反应,交叉配血是国家《临床输血技术规范》中明确要求检查的项目,交叉配血不合时必须按有关规定做抗体筛选试验,这对于保障输血安全、提高输血治疗效果、减少输血不良反应起到了非常重要的作用。抗人球蛋白试验是检测IgG抗体的金标准方法,间接抗人球蛋白试验是检测IgG抗体的有效方法,目前临床在用的绝大多数属于凝胶法抗人球蛋白试验,该方法采用的增强液是LISS液,虽然已经将经典盐水37℃孵育的时间从30~60分钟缩短到15分钟,但对于临床尤其是急救情况下,时间显得尤为重要,15分钟仍然太长。
发明内容
本发明所要解决的技术问题是针对上述现有技术提供低离子增强液的制备方法,缩短了孵育时间,提高了特异性和灵敏度 。
本发明的目的是这样实现的:
一种低离子增强液的制备方法,包括以下几个步骤:
步骤一、缓冲液的配方如下:
磷酸二氢钾 (130-180)mmol
磷酸氢二钠 (130-180)mmol
以上试剂用纯化水分别溶解;
步骤二、低离子增强液的配方如下:
(130-180)mmol磷酸二氢钾 (10-12)x10-3ml/ml
(130-180)mmol磷酸氢二钠 (7-10)x10-3ml/ml
氯化钠 (1.6-1.9)x10-3g/ml
甘氨酸 (15-20)x10-3g/ml
对羟基苯甲酸甲酯 (5-9)x10-4g/ml
对羟基苯甲酸丙脂 (0. 8-1.5)x10-4g/ml
牛血清白蛋白 (1-4)x10-3g/ml
以上试剂用纯化水溶解,调节pH为6.4-7.0。
与现有技术相比,本发明的优点在于:对羟基苯甲酸甲酯和对羟基苯甲酸丙脂所具有的酚羟基结构,可以破坏微生物的细胞膜,使细胞内的蛋白值变相,有效抑制微生物的生长,防止凝胶或抗体因为细菌繁殖而失效;牛血清白蛋白带有正电荷,能中和细胞表面的负电荷,降低红细胞表面电荷量,减少红细胞之间的排斥力,缩短红细胞之间距离,同时也具有强亲水性,可破坏红细胞表面亲水层,降低抗体结合红细胞抗原时所产生的空间排斥效应,所以在保障特异性和灵敏度的前提下能缩短37℃孵育时间;低离子液提供的低离子环境可以增加带正电荷的抗体与带有负电荷的抗原相互碰撞产生特异性结合形成抗原抗体复合体的机会,促进抗体与抗原结合效率,缩短抗原抗体结合的时间。因此可以加速抗体与红细胞上相应抗原的结合,同时提高抗原与抗体尤其是IgG的反应强度,进一步减少试验孵育时间,提高凝集效果。
Claims (2)
1.低离子增强液的制备方法,其特征在于:
所述制备方法包括以下几个步骤:
步骤一、缓冲液的配制
磷酸二氢钾 (130-180)mmol
磷酸氢二钠 (130-180)mmol
步骤二、低离子增强液的配方如下:
(130-180)mmol磷酸二氢钾 (10-12)x10-3ml/ml
(130-180)mmol磷酸氢二钠 (7-10)x10-3ml/ml
氯化钠 (1.6-1.9)x10-3g/ml
甘氨酸 (15-20)x10-3g/ml
对羟基苯甲酸甲酯 (5-9)x10-4g/ml
对羟基苯甲酸丙脂 (0. 8-1.5)x10-4g/ml
牛血清白蛋白 (1-4)x10-3g/ml
以上试剂用纯化水溶解,调节pH为6.4-7.0。
2.以上述试剂中的一种或几种配制而成的用于抗人球蛋白试验,达到缩短了孵育时间,提高了特异性和灵敏度目的的均属该专利包含的范围 。
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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EP1840572A1 (en) * | 2006-03-30 | 2007-10-03 | Stiftung Für Diagnostische Forschung | A system for the enhancement of the detection of antibodies directed against red cell antigens |
CN101718786A (zh) * | 2009-11-25 | 2010-06-02 | 江阴力博医药生物技术有限公司 | 抗人球蛋白交叉配血卡的制备方法 |
CN108548921A (zh) * | 2018-03-13 | 2018-09-18 | 中国人民解放军第二军医大学第二附属医院 | 一种间接抗人球蛋白加速配血的试验方法 |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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EP1840572A1 (en) * | 2006-03-30 | 2007-10-03 | Stiftung Für Diagnostische Forschung | A system for the enhancement of the detection of antibodies directed against red cell antigens |
CN101718786A (zh) * | 2009-11-25 | 2010-06-02 | 江阴力博医药生物技术有限公司 | 抗人球蛋白交叉配血卡的制备方法 |
CN108548921A (zh) * | 2018-03-13 | 2018-09-18 | 中国人民解放军第二军医大学第二附属医院 | 一种间接抗人球蛋白加速配血的试验方法 |
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Application publication date: 20190621 |