CN109893473B

CN109893473B - Deep ocean concentrate skin care kit and application thereof

Info

Publication number: CN109893473B
Application number: CN201810883066.8A
Authority: CN
Inventors: 苏珍仪
Original assignee: Individual
Current assignee: Su Zhenyi
Priority date: 2017-12-08
Filing date: 2018-08-06
Publication date: 2022-07-26
Anticipated expiration: 2038-08-06
Also published as: TW201924648A; CN109893473A; TWI791587B

Abstract

The invention relates to a deep-sea concentrated skin care kit containing a deep-sea concentrate and a skin care formula. The deep ocean concentrate skin care kit of the present invention has: 1. instantly micronizing skin care formulation; 2. the high surface tension of the deep sea concentrate is immediately reduced, and the problem that the deep sea concentrate is not easy to absorb by the skin is solved; 3. one of the physical properties of the concentrate of the deep ocean layer is the Driving Force for penetration of the micronized skin care formulation (drying Force), which makes the skin (dermis) more easily absorbed. The present invention further provides a method of making a deep ocean concentrate skin care kit that results in improved skin appearance.

Description

Skin care kit with deep ocean concentrate and application thereof

[ technical field ] A method for producing a semiconductor device

The invention relates to a skin care kit containing a deep ocean concentrate and a skin care formula, in particular to a composition of the deep ocean concentrate and the skin care formula which is prepared in real time.

[ background of the invention ]

Deep Seawater (DSW) is low-temperature, clean and pollution-free seawater below sea level by 200m, and is rich in metal ions such as magnesium, calcium and potassium, nutrient salts and inorganic components with high solubility and difficult decomposition. The deep seawater is rich in minerals, mainly because sunlight cannot penetrate through the deep seawater, photosynthesis cannot occur, so that inorganic nutrient salts are not consumed by phytoplankton, and the deep seawater has higher content of the inorganic nutrient salts compared with surface seawater. Because of the abundant inorganic nutritive salt, deep seawater is widely used in health beverages, foods, medical products and aquaculture at present.

In recent years, deep seawater has been widely used in food processing industry, agriculture, and medicine. Research literature indicates that the deep seawater can reduce blood fat, lipid oxidation, angiosclerosis and hypertension, and can also reduce vascular wall hyperplasia; although it has been found that patients who drink or soak deep seawater can improve the symptoms of skin inflammation and allergy in the research of atopic dermatitis, it has not been applied to the cosmetic industry on a large scale. In addition, drinking deep ocean water has also been found to improve cataracts.

Since deep ocean water is only available in four parts of the world, taiwan has successfully collected and made concentrated solutions, which can be eaten and used as a health product. In the cell experiments, it has been shown that deep seawater can stimulate the regeneration of collagen, whiten skin, and inhibit or treat inflammation, but deep seawater has not been used in large quantities in the addition of cosmetics and care products. Generally, the commercially available skin care products have large molecular groups and are not easily absorbed by the skin because of stable oil-water fusion by an emulsifier, while the deep ocean concentrate has a balanced and stable ionic composition, is easily absorbed by cells, but has a high surface tension characteristic, so that the deep ocean concentrate is not easily penetrated into the skin. At present, no effective product of the health care product directly mixed with the deep sea concentrated solution is disclosed in the literature. In order to provide more effective skin care products and increase the industrial value of deep ocean water, there is a need to provide a composition containing deep ocean concentrate (liquid/powder) and skin care formula.

[ summary of the invention ]

Accordingly, the present invention provides a skin care kit comprising a deep marine concentrate for improving skin health or appearance, and a skin care formulation, methods of using the same, and methods of making the same. The skin care kit of the deep ocean concentrate is characterized by immediately micronizing the skin care formula, immediately reducing the high surface tension of the deep ocean concentrate, solving the problem that the skin is not easy to absorb and the material property of the deep ocean concentrate can be the penetration Driving Force (Driving Force) of the micronized skin care formula so as to ensure that the skin (dermis) is easier to absorb.

The invention provides a skin care kit of a deep sea concentrate, which comprises a first agent and a second agent, wherein the first agent is the deep sea concentrate, the second agent is a skin care formula, and the first agent and the second agent are independently packaged.

The invention provides a skin care kit of a deep sea concentrate, wherein the concentration of magnesium in the deep sea concentrate is 6,500-110,000 mg/l.

The invention provides a skin care and maintenance kit of a deep sea concentrate, wherein the deep sea concentrate has a sodium concentration of 3,800-30,000 mg/l, a potassium concentration of 400-18,000 mg/l and a calcium concentration of 100-500 mg/l.

The invention provides a skin care kit of a deep sea concentrate, wherein the deep sea concentrate has a hardness of 80,000-420,000 mg/l and a salinity of 380-430 per mill.

The invention provides a skin care kit of a deep sea concentrate, wherein each 100g of the deep sea concentrate contains 1,000-1,500 mg of calcium, 1,400-2,100 mg of magnesium, 2,000-4,000 mg of sodium, 1,400-2,100 mg of potassium and sulfate radicals less than 6.5 wt%, and the specific gravity of the deep sea concentrate is 1.17-1.32g/cm ³ The marine mineral solution of (a).

The present invention provides a deep ocean concentrate skin care kit, wherein the deep ocean concentrate is taken from a source of water below 200m of the ocean.

The present invention provides a skin care kit comprising a deep ocean concentrate, wherein the deep ocean concentrate is taken from a source of water below 500m of the ocean.

The invention provides a skin care and maintenance kit of a deep sea concentrate, wherein the skin care and maintenance formula comprises whitening products, moisturizing products, wrinkle removing products, wound healing products, medical or beauty leading-in products, collagen hyperplasia promoting products and other products for improving skin appearance.

The invention also provides a skin care kit of the deep sea concentrate, wherein the deep sea concentrate is liquid or powder.

The invention also provides a skin care kit of the deep sea concentrate, wherein the skin care formulation is in a gel or cream shape.

The present invention also provides a method of promoting penetration of a skin care formulation, (a) providing a skin care formulation; (b) mixing the skin care formulation with the deep ocean concentrate prior to use; the skin care formulation is immediately micronized to increase the penetration of the skin care formulation, which can rapidly enter the skin to reach the dermis.

The invention also provides a method for promoting the penetrability of the skin care and care formula, wherein the concentration of magnesium in the deep sea concentrate is 6,500-110,000 mg/l.

The invention also provides a method for promoting the penetrability of the skin care and care formula, wherein the concentration of sodium, potassium and calcium in the deep sea concentrate is 3,800-30,000 mg/l, 400-18,000 mg/l and 100-500 mg/l.

The invention also provides a method for promoting the penetrability of the skin care and maintenance formula, wherein the hardness of the deep sea concentrate is 80,000-420,000 mg/l, and the salinity is 380-430 per mill.

The invention also provides a method for promoting the penetrability of the skin care and care formula, wherein the deep sea concentrate is liquid or powder.

The present invention also provides a method of promoting penetration of a skin care formulation, wherein the skin care formulation is in the form of a gel or cream.

The present invention further provides a method of enhancing penetration of a skin care formulation, wherein the concentration of the deep ocean concentrate effective after mixing with the skin care formulation is at least greater than or equal to 1.25 (v/v)%.

The invention also provides a method for promoting the penetrability of the skin care and maintenance formula, wherein the deep ocean concentrate is mixed with the skin care and maintenance formula and then immediately smeared on an affected part.

The present invention further provides a method of promoting penetration of a skin care formulation, wherein the affected area is skin.

The invention also provides a method for caring skin, which comprises the step of administering the deep sea concentrate skin caring kit to skin immediately after preparation.

The invention also provides a method for caring and maintaining skin, wherein the effective concentration of the deep sea concentrate is at least greater than or equal to 1.25 (v/v)%, after the deep sea concentrate is mixed with the skin caring and maintaining formula.

The invention also provides a method for caring and maintaining the skin, wherein the deep ocean concentrate skin caring and maintaining kit can quickly enter the skin to reach the dermis layer and achieve the effects of removing wrinkles, whitening, compacting and the like on improving the appearance of the skin.

To achieve the foregoing and other objects, one or more specific embodiments of the present invention are described below. Further features or advantages of the invention are described in detail in the description of the embodiments and the claims.

[ description of the drawings ]

Fig. 1A-1C show the change before and after application of the "deep ocean concentrate skin care kit" to a wound, wherein fig. 1A is a photograph of the wound before application. Fig. 1B is a photograph of a wound on which the deep ocean concentrate skin care kit has just been applied. Fig. 1C is the wound after several hours of application of the deep ocean concentrate skin care kit.

Fig. 2A-2G show the photographs taken with an optical microscope at 40 times and 200 times, which respectively observe the deep sea concentrated solution (fig. 2A), the maintenance gel (fig. 2B), the maintenance cream (fig. 2C-1 and 2C-2), the real-time mixed deep sea concentrated solution and maintenance gel (deep sea concentrated solution volume: maintenance gel volume: 1) (fig. 2D-1 and 2D-2), the real-time mixed deep sea concentrated solution and maintenance cream (deep sea concentrated solution volume: maintenance cream volume: 1) (fig. 2E-1 and 2E-2), the mixed deep sea concentrated solution and maintenance gel (deep sea concentrated solution volume: 1) (fig. 2F-1 and 2F-2) after standing for 3 hours, the mixed deep sea concentrated solution and maintenance cream (deep sea concentrated solution volume: maintenance cream volume: 1) (fig. 2G -1, 2G-2).

Fig. 3A-3C show the change in physical properties of deep ocean concentrate after 10 days of mixing with a skin care formulation. FIG. 3A shows that the deep ocean concentrate forms a white lump precipitate after 10 days of mixing with the cosmetic gel. FIG. 3B shows that after mixing the deep ocean concentrate with the maintenance gel for 10 days, the gel disintegrates and the viscosity of the gel is significantly reduced. Fig. 3C shows that after mixing the deep ocean concentrate with the skin care cream for 10 days, the cream disintegrates and the stickiness of the cream is significantly reduced.

FIGS. 4A-4B show the improvement of facial texture and streaking by iontophoresis of a skin care kit of ocean deep concentrate on the face of a patient.

FIGS. 5A-5B are graphs showing dynamic laser particle size analysis, wherein FIG. 5A is a graph showing particle size analysis of a deep sea concentrate; fig. 5B is a particle size analysis chart of the real-time mixed deep ocean concentrated solution and maintenance gel (weight of deep ocean concentrated solution: weight of maintenance gel: 1).

Fig. 6A shows a graph of particle size change at different time points after mixing the deep ocean concentrate with the maintenance gel (weight of deep ocean concentrate: weight of maintenance gel: 1) in real time.

FIG. 6B shows a particle size microscopic view of the maintenance gel.

Fig. 6C shows a microscopic observation image of the particle size of the mixture of purified water and a preservative gel (purified water: preservative gel weight: 1).

Fig. 6D shows a microscopic view of micelle particles obtained by mixing the deep sea concentrated solution with a maintenance gel (the weight of the deep sea concentrated solution: the weight of the maintenance gel: 1).

[ detailed description ] embodiments

The invention provides a skin care kit of a deep ocean concentrate.

The deep seawater in the invention is seawater which is taken from 200 to 1500 meters below sea level, preferably 500 to 700 meters below sea level, and each 100g of solution (liquid/powder) contains 1000 to 1500mg of calcium, 1400 to 2100mg of magnesium, 2000 to 4000mg of sodium, 1400 to 2100mg of potassium and sulfate radical which are less than 6.5 wt%, and the specific gravity of the deep seawater is 1.17 to 1.32g/cm ³ A solution of marine minerals of (a).

The deep sea concentrate in the invention refers to liquid or powder produced by concentrating deep sea water. The deep sea concentration process includes fiber filtering system, ultrafiltering system, reverse sea water permeating system, low temperature vacuum evaporating and concentrating system and/or centrifuging.

The deep sea concentrate has a hardness of 80,000-420,000 mg/l, a salinity of 380-430 ‰, a magnesium concentration of 6,500-110,000 mg/l, and a sodium concentration of 3,800-30,000 mg/l. The deep ocean concentrate of the present invention is rich in various minerals including, but not limited to, trace elements such as calcium (Ca), potassium (K), iron (Fe), zinc (Zn), molybdenum (Mo), manganese (Mn), lithium (Li), strontium (Sr), copper (Cu), silicon (Si), etc. In one embodiment, the composition of the concentrate is shown in table one, but the concentrate of the present invention is not limited thereto since the detection unit does not detect the complete elements.

Ingredients of epi-I, deep sea concentrate

The skin care and care formula in the invention refers to a formula for a treatment course used by general care products and beauty-related institutions, and can be a cream or gel care product. For example, skin whitening products, moisturizing products, wrinkle removing products, wound healing products, quick healing treatment and other products for improving the appearance of skin.

The "mixture of the deep ocean concentrate and the skin care and care formula" or the "deep ocean concentrate mixture" of the present invention refers to a mixture of the deep ocean concentrate and the skin care and care formula. The concentrated solution can be added into gel or cream-like skin care product to immediately generate phase transfer to reduce molecular size, so that the skin care product can be easily absorbed by skin to reach deep layer of skin to exert effect.

However, deep ocean concentrates, when added to skin care formulations for a period of time, can cause the skin care formulations to deteriorate, destabilize and disintegrate. Thus, the mixture of the deep ocean concentrate of the present invention and the skin care and care formulation is preferably freshly prepared and used within 10 days, preferably within 9 days, 8 days, 7 days, 6 days, 5 days, 4 days, 3 days, 2 days, 1 day after mixing, most preferably within 12 hours, 6 hours, 3 hours, 2 hours, 1 hour.

When the deep ocean concentrate is mixed with the skin care formulation, the surface tension of the mixture (the deep ocean concentrate and the skin care formulation) can be reduced, and the absorption of the skin (dermis) into the care kit can be increased. Therefore, the mixture is easily absorbed by the skin and reaches the dermis to exert the effect.

The present invention also provides a method of enhancing penetration of a skin care formulation comprising providing a skin care formulation and mixing the skin care formulation with a deep ocean concentrate prior to use.

The mixing method is not particularly limited, and a general method such as stirring, shaking, tube shaking, ultrasonic wave, etc. may be used as long as both can be uniformly mixed. The mixing time is not particularly limited, and the mixing time is not preferably too long, usually within 15 minutes, preferably within 14, 13, 12, 11, 10, 9, 8, 7, 6,5, 4, 3 minutes, since it is necessary to use the skin care formulation as soon as possible after mixing to avoid deterioration of the quality of the skin care formulation. The mixing time can be adjusted appropriately according to the skin care formulation and the mixing method used.

As mentioned above, the deep ocean concentrate must be applied to the skin as soon as possible after mixing with the skin care formulation in order to avoid quality changes in the skin care formulation.

After the deep sea concentrate is added into a skin care and care formula, the gel and cream care product is obviously deteriorated after only 10 days. In addition, the deep ocean concentrate can cause the gel care product to generate unknown precipitates and floating matters, and shows that if the deep ocean concentrate is directly added into the skin care formula for a period of time, ions and components of the deep ocean concentrate can generate chemical changes, the effect of the original skin care formula on skin is reduced, and even possible harmful skin derivatives are generated. Therefore, the deep sea concentrate and the maintenance product must be separately packaged and freshly prepared before use, so that the synergistic effect can be achieved.

The deep sea concentrate can be added into skin care formula to generate phase transition immediately, so that the molecules of the skin care formula are reduced, and the skin care formula can be easily absorbed by skin to reach the dermis. Therefore, the deep ocean concentrate can promote the skin penetration of the skin care and care formula sold in the market, and accelerate the effect of the skin care and care formula.

The present invention further provides a method of caring skin comprising administering the deep ocean concentrated mixture of the present invention to the skin immediately after the preparation thereof. The deep sea concentrated mixture can reach the inside of the skin quickly and be absorbed by the body after being smeared or introduced to the skin through an instrument, and has the effect.

[ examples ] A method for producing a compound

The preparation method of the deep sea concentrate comprises:

the preparation method of the deep sea concentrate comprises the following steps: firstly, providing deep seawater at a preset depth from the sea level, filtering the deep seawater by using a fiber filtering system, an ultrafiltration system and a seawater reverse osmosis system in sequence to obtain a first concentrated solution and purified water, and concentrating the first concentrated solution by using a low-temperature vacuum evaporation concentration system to obtain a second concentrated solution and calcium sulfate (gypsum). Since the low-temperature vacuum evaporation concentration system is a circulating system, the second concentrated solution can be continuously concentrated by the system. During the concentration process, the second concentrated solution will generate crystallized salts, so that solid granular salt (deep sea salt) and the third concentrated solution can be obtained by centrifugation. Heating the third concentrated solution at a predetermined high temperature (90-120 ℃), wherein the third concentrated solution continuously separates out crystal salt in the heating process, standing the heated third concentrated solution to precipitate the crystal salt in the lower layer, and drawing the supernatant to obtain a fourth concentrated solution. Finally, filtering the cooled fourth concentrated solution by using a filter membrane with a predetermined pore size (0.5-1.5 mu m) to obtain a seawater deep-layer concentrate.

The deep sea concentrated mixture with different effects is prepared according to the material characteristics of different formulas of the deep sea concentrated product and the properties of the nursing formula, and the effects of whitening, moisturizing, wrinkle removing, wound healing and the like of the mixture can be optimized.

Various formulations of deep ocean concentrates (liquids/powders) were prepared by the above process and reasonable intervals were selected from each formulation as shown in table two below.

Ingredient contents of deep ocean concentrates (liquid/powder) with different formulas

Example 1 wound test

The mixture of the real-time mixed deep sea concentrated solution and the maintenance gel is smeared on the injured leg, and the wound change is observed.

Referring to fig. 1A-1C, the legs were injured at 10:16 am on 23.9.7.2017 (see fig. 1A), and the skin care kit of deep ocean concentrated liquid/powder "mixing the deep ocean concentrated liquid and care gel in real time" was applied to the sterilized wounds at 3:56 pm (see fig. 1B), and the wounds subsided red swelling and no scab was generated at 10:45 night (see fig. 1C).

From these results, it was found that mixing the skin care gel with the deep ocean concentrated solution accelerates skin absorption and promotes wound healing.

Example 2 Effect of deep ocean concentrate on cream maintenance and gel penetration

Sucking the mixture of deep sea concentrated solution and cream or gel with quantitative dropper. The control group is (a) water, (b) deep sea concentrated solution, (c) gel, or (d) cream. The experimental groups are 1mL of deep ocean concentrated solution (1 time, 5 times, 10 times, 20 times and 40 times) and 1mL of cream or gel, and the effective concentration in the final mixture of the deep ocean concentrated solution is 50 (v/v)%, 10 (v/v)%, 5 (v/v)%, 2.5 (v/v)% and 1.25 (v/v)%.

On the penetration test regular cloth AATCC97, 0.5mL of the mixture was dropped on regular cloth AATCC97 at a fixed distance of 1cm in height. After the mixture of the 'deep sea concentrated solution and the gel' is dripped, the time required for the mixture to completely penetrate the test cloth is counted; on the other hand, after dropping the mixture of "deep ocean water concentrate and cream", the mixture was observed for penetration at a timing of 2 minutes, and the results are shown in table three.

Comparing penetration force of the skin care product and the deep sea concentrated solution before and after mixing

As shown by the experimental results of (b), the ionic deep sea concentrated solution has many documents to prove that it has skin whitening and anti-aging effects on skin cells, and as many other components for skin care, it has high surface tension and is difficult to absorb and penetrate skin cells.

However, after the deep sea concentrate is mixed with the maintenance gel or the maintenance cream in a volume of 1:1, the molecular group can be reduced, the penetrating power and the absorption power of the maintenance product can be immediately improved, the high surface tension of the deep sea concentrate can be immediately reduced, and the problem that the skin is not easy to absorb is solved. To produce excellent permeability while increasing the absorption rate of the skin and achieving better results.

Example 3 Effect of deep ocean concentration on skin Care formulation size

The experiment was conducted by the institute of Industrial and research, materials and chemical institute, and photographed by an optical microscope, the deep sea concentrated solution (fig. 2A), the maintenance gel (fig. 2B), the maintenance cream (fig. 2C), the real-time mixed deep sea concentrated solution and maintenance gel (the volume of the deep sea concentrated solution: the volume of the maintenance gel: 1) (fig. 2D), the real-time mixed deep sea concentrated solution and maintenance cream (the volume of the deep sea concentrated solution: the volume of the maintenance cream: 1) (fig. 2E), the mixed deep sea concentrated solution and maintenance gel (the volume of the deep sea concentrated solution: the volume of the maintenance gel: 1) (fig. 2F) after standing for 3 hours, and the mixed deep sea concentrated solution and maintenance cream (the volume of the deep sea concentrated solution: the volume of the maintenance cream: 1) (fig. 2F) after standing for 3 hours were observed respectively.

The maintenance gel (fig. 2B) and the maintenance cream (fig. 2C-1) were observed under a microscope at a magnification of 40 times and had a compact molecular mass without granular particles. The maintenance cream was observed at 200 x (see fig. 2C-2), and was also not visibly grainy.

Observing under microscope of 40 times and 200 times, mixing the deep sea concentrated solution and caring gel (shown in figure 2D-1 and figure 2D-2) and mixing the deep sea concentrated solution and caring cream (shown in figure 2E-1 and figure 2E-2) in real time, wherein the caring gel and caring cream with compact molecular groups are immediately micronized by charged mineral ions to be 3 times or less of the original size and uniformly distributed. In conclusion, mixing the deep ocean concentrate and the skin care gel/cream in real time can result in clear encapsulation and uniform mixing of oil and water, which makes it easier to be absorbed by the skin.

However, when the mixed deep sea concentrated solution and the caring gel (see fig. 2F-1, fig. 2F-2) and the mixed deep sea concentrated solution and caring cream (see fig. 2G-1, fig. 2G-2) are observed under a microscope of 40 times and 200 times for 3 hours, the molecular clusters are obviously increased, and when the mixed deep sea concentrated solution and caring gel (see fig. 2F-2, fig. 2G-2) and the mixed deep sea concentrated solution and caring cream (see fig. 2F-2, fig. 2G-2) are observed under a microscope of 200 times for 3 hours, the phenomenon of the molecular clusters are more likely to be found, and the skin absorption is more difficult.

From the above experiments, it can be known that the deep ocean concentrate of the present application has the efficacy of instantly micronizing skin care and care formulations. The maintenance gel and the maintenance cream which respectively exist independently are macromolecules, have compact molecular groups, and are not easy to be absorbed by skin. The mixed deep ocean concentrate and the maintenance gel/maintenance cream after being left for a period of time have the phenomenon of molecular clustering, which makes it more difficult to be absorbed. Only mixing the deep ocean concentrate and the skin care gel/cream in real time can make the respective molecules micronized and mixed uniformly, which can be absorbed by the skin more easily.

Example 4 Effect of deep ocean concentrate on cream and gel body maintenance and stability

Respectively filling 1mL of cream and gel into a micro centrifuge tube, centrifuging at 2000rpm for 5 minutes, and sequentially diluting the deep sea concentrated solution with sterilized ultrapure water by 1 time, 5 times, 10 times, 20 times and 40 times. Taking 1mL of deep sea concentrated solution with different dilution times, respectively adding into a microcentrifuge tube filled with 1mL of cream or gel, and mixing. The control groups respectively comprise (a)1mL of ultrapure water +1mL of gel, (b)0.5mL of ultrapure water +0.5mL of cream; the experimental groups are 1mL of deep ocean concentrated solution (1 time, 5 times, 10 times, 20 times and 40 times) and 1mL of cream or gel. The effective concentration in the final mixture of the deep ocean layer concentrated solution is 50 (v/v)%, 10 (v/v)%, 5 (v/v)%, 2.5 (v/v)% and 1.25 (v/v)%. The mixture was mixed vigorously using a test tube shaker, placed on a test tube stand at room temperature for 10 days, and the viscosity (viscocity) of the mixture was measured using a glass capillary. Fixing the microcentrifuge tube filled with the mixture on a table top, taking a glass capillary tube to go deep into the microcentrifuge tube, timing for 1 minute, taking out the capillary tube, and measuring the height of the fluid in the tube.

Referring to fig. 3A, the viscosity of the deep ocean concentrate was significantly changed 10 days after mixing with the maintenance gel, which indicates that the physical properties have changed and that the precipitation of white lumps is clearly visible under visual observation, indicating that the chemical change of the mixture occurred during 10 days of standing.

Referring to fig. 3B, after the deep ocean layer concentrate is mixed with the skin care cream, the viscosity of the cream is significantly reduced through visual and experimental tests, which indicates that the stability of the cream is changed.

Referring to FIG. 3C, 10 days after the deep ocean concentrate was mixed with the maintenance gel, it was found that the gel was disintegrated and the consistency was reduced by 100%, and from FIG. 3C, it was found that the capillary height was 1.5cm, representing a gel phase, when the deep ocean concentrate was not present; when the deep sea water concentrated solution with high or low concentration is added, even the deep sea water concentrated solution diluted by 40 times (the concentration of the deep sea water concentrated solution is 1.25%), the capillary height is 3cm, which represents that the water phase is changed.

As can be seen from the above, the deep sea concentrate (liquid/powder) may cause the oil-water separation of the skin care formula to be unstable, even may generate chemical crystallization quality change during long-term storage, and is not confirmed to be beneficial to skin health, so the deep sea concentrate cannot be stored for a long time after being mixed with the skin care cream or gel, and the best use method is suggested to be that the deep sea concentrate is equally mixed before use and then applied to skin.

EXAMPLE 5 real-time blending of Marine concentrates and maintenance gel non-invasive Water needle introduction test

In 2017, 10 and 15 months, a mixture of the marine concentrate and the maintenance gel is introduced into the right face by using a medical-grade iontophoresis instrument. The right face was reintroduced at 26 days 10 months. After the skin is introduced into the right face in 10 months and 15 days, the right face turns red, and the phenomenon of the skin appearance shows that high-concentration ions in the deep ocean concentrated solution penetrate into the dermis layer and even the basal layer after the introduction, so that the local capillary blood circulation of the deep skin is increased, the skin temperature is slightly increased, the skin metabolic rate is effectively promoted, the red phenomenon disappears within a few hours, and compact and smooth skin textures are presented (as shown in figure 4A). The right face was introduced again at 26 days 10 months, and the eyebrow height was changed by inserting the right face into the anterior and posterior regions and pulling it up. The proportions of the introduced anterior and posterior facial textures, superficial layer plaques, purpurins and brown plaques were analyzed as shown in Table four.

TABLE IV comparison of facial skin before and after introduction

	10 months and 15 days	10 months and 26 days	Changes to
				Texture of	51％	61％	10％
Superficial spots	88％	91％	3％
				Purple matter (deep layer spot)	89％	92％	3％
Brown colored spot (basal)	47％	67％	20％

The above experimental results show that the effects of improving skin firmness, reducing fine wrinkles and skin luster can be affected after the skin is introduced into the deep layer of cells by using an iontophoresis instrument for 24 hours. The material characteristics of the deep sea concentrate can become a Driving Force (Driving Force) for skin to permeate with the nursing and caring formula after the skin is micronized, so that the skin (corium layer) is easier to absorb, the basal layer is really repaired quickly, and the effects of quickly repairing, immediately increasing the water retention capacity of the horny layer and obviously improving the culture are achieved.

Therefore, compared with the maintenance gel used alone, the maintenance gel mixed with the marine concentrated solution can accelerate the skin absorption and quickly reach the dermis layer or even the basal layer to produce the treatment effect.

EXAMPLE 6 Effect of deep ocean concentrate on gel particle size maintenance

The research institute of the institute of Industrial science and chemistry, as an example of the experiment, measured the particle size with a dynamic light scattering particle size analyzer, and examined the deep ocean concentrated solution (as shown in fig. 5A) and the real-time mixed deep ocean concentrated solution and maintenance gel (weight of deep ocean concentrated solution: weight of maintenance gel: 1) (as shown in fig. 5B).

A dynamic light scattering particle size analyzer uses laser light to irradiate a solution containing particles, scattered light is generated when the laser light impinges on the particles, and the change of the scattered light with time is measured to calculate the particle size distribution of the particles.

The dynamic laser particle size analysis showed that the particle size distribution of the deep ocean concentrated solution (as shown in fig. 5A) was mainly 0.89nm, the signal was the highest, and the overall average particle size was 19.2nm (19.2 nm), indicating that the sample was a liquid without obvious particles.

As shown in the dynamic laser particle size analysis chart, when the deep ocean concentrated solution and the maintenance gel (the weight of the deep ocean concentrated solution: the weight of the maintenance gel is 1:1) are mixed in real time (as shown in fig. 5B), micelle begins to form, the distribution of main particles shows that 5082nm has the highest signal value according to the analysis result, and the overall average particle size is 5.2 μm (Z average is 5.2 μm). As can be seen from fig. 5B, the distribution of the sample signal values is single and concentrated, which indicates that uniform size micelle particles can be formed immediately after mixing the deep ocean concentrated solution and the maintenance gel (the weight of the deep ocean concentrated solution: the weight of the maintenance gel: 1) in real time.

TABLE V, dynamic laser particle size analysis proportion distribution table of the deep sea concentrated solution and the real-time mixed deep sea concentrated solution and maintenance gel

Example 7 Effect on particle size in short time after real-time blending of deep ocean concentrate with maintenance gel

The experiment was conducted by the institute of materials and chemical industry, and the particle size of the sample was observed using an optical microscope and a dynamic light scattering particle size analyzer, and the change in particle size was detected at different time points after mixing the deep ocean layer concentrate and the maintenance gel (weight of deep ocean layer concentrate: weight of maintenance gel: 1) (see fig. 6A).

Observing the 0 second, 10 seconds, 20 seconds, 30 seconds, 1 minute, 2 minutes, 3 minutes and 5 minutes after the deep sea concentrated solution and the maintenance gel (the weight of the deep sea concentrated solution: the weight of the maintenance gel is 1:1) are mixed in real time under an optical microscope with a magnification of 200 times, and observing a sample by using a fixed visual field.

TABLE VI, dynamic laser particle size analysis table for different time points after mixing the deep sea concentrated solution and the maintenance gel in real time

It can be concluded from the above experiments of optical microscope and laser particle size that the effect of micelles is completed after the deep ocean layer concentrate and the maintenance gel are mixed in real time, and the average particle size of micelles is stable within 5 minutes after mixing, which means that the size of particles will not be changed by the increase of time after the deep ocean layer concentrate and the maintenance gel are uniformly mixed in a short time, and the composition is suitable for being immediately applied to human skin after mixing.

In addition, differences of micelle particles were observed under an optical microscope at 200 × magnification among a single dose of the maintenance gel (see fig. 6B), a real-time mixture of pure water and the maintenance gel (weight of pure water: weight of maintenance gel: 1) (see fig. 6C), and a real-time mixture of the deep ocean concentrated solution and the maintenance gel (weight of deep ocean concentrated solution: weight of maintenance gel: 1) (see fig. 6D).

As seen from the observation by an optical microscope, the cream bodies of the maintenance gel (fig. 6B) prepared by single-dose, and the maintenance gel prepared by mixing pure water and the maintenance gel in real time (weight of pure water: maintenance gel is 1:1) (fig. 6C) were not uniformly dispersed, and the effect of particle micelles was effectively generated, so that the aggregation phenomenon was remarkable. On the other hand, when the deep sea water concentrate and the maintenance gel (weight of deep sea water concentrate: weight of maintenance gel: 1) were mixed in real time (see fig. 6D), Phase Transfer (Phase Transfer) occurred immediately in the cream texture of the maintenance gelatin and micelle particles were formed, and the average particle size was about 5.03 μm. Compared with the common care product with the particle size of 15-50 μm, the particle size of the care product molecular group can be reduced by one third to one tenth, which is beneficial to improving the probability that the care product molecular group can enter the deep layer of the skin more effectively.

EXAMPLE 8 real-time Propulsion Force (Driving Force) measurement of blending ocean deep concentrate with maintenance gel

The experiment was conducted by the institute of materials and chemical industry, and the real-time mixture of the deep ocean concentrated solution and the maintenance gel (the weight of the deep ocean concentrated solution: the weight of the maintenance gel: 1) was examined for conductivity by using a conductivity analyzer, thereby explaining the propulsive force.

The conductivity of the mixture was measured by a conductivity analyzer, which was used to control (a) pure water and (b) a real-time mixture of pure water and maintenance gel (pure water: maintenance gel weight: 1) and (c) a real-time mixture of the deep ocean concentrate and maintenance gel (deep ocean concentrate weight: maintenance gel weight: 1), and the samples were mixed in real time and then directly measured by the analyzer.

Conductivity test of Epimedium, deep ocean concentrate and different mixtures

From the conductivity test, it was found that the conductivity of the real-time mixed ocean deep concentrated solution and maintenance gel (weight of ocean deep concentrated solution: weight of maintenance gel: 1) of the experimental group (c) is more than 20 times that of the real-time mixed pure water and maintenance gel (weight of pure water: weight of maintenance gel: 1) of the experimental group (b). Compared with pure water of the control group (a), the conductivity is even 12,250 times, and the obvious difference of the conductivity shows that the real-time mixing of the deep sea concentrated solution and the maintenance gel (the weight of the deep sea concentrated solution: the weight of the maintenance gel: 1) provides good propulsive Force (Driving Force) of the mixture, so that the human body is promoted to effectively absorb the maintenance products to enter the deep layer of the skin through the propulsive Force, and the efficacy of the skin care and maintenance formula is improved.

All of the features disclosed in the present application may be implemented in any combination. Each feature disclosed in this specification may be replaced by an alternative feature serving the same, equivalent, or similar purpose. Thus, unless expressly stated otherwise, each feature disclosed is one example only of a generic series of equivalent or similar features.

Claims

1. A deep sea concentrate skin care kit is characterized by comprising a first agent and a second agent, wherein the first agent is a deep sea concentrate, and the second agent is a skin care formula; wherein the effective concentration of the deep ocean concentrate is 1.25-50v/v% after the deep ocean concentrate is mixed with the skin care formula; the hardness of the deep sea concentrate is 80,000-420,000 mg/l, and the salinity is 380-430 per mill; the magnesium concentration of the deep sea concentrate is 6, 500-110, 000 mg/l; the sodium concentration is 3, 800-30, 000mg/l, the potassium concentration is 400-18, 000mg/l, and the calcium concentration is 100-500 mg/l; the skin care formulation is in a gel or cream state.

2. The deep sea concentrate skin care kit according to claim 1, wherein the deep sea concentrate comprises 1000-1500 mg of calcium, 1400-2100 mg of magnesium, 2000-4000 mg of calcium per 100gSodium, 1400-2100 mg of potassium, sulfate radical < 6.5 wt%, and specific gravity of 1.17-1.32g/cm ³ A solution of marine minerals of (a).

3. The deep ocean concentrate skin care kit of claim 1 wherein the deep ocean concentrate is taken from a source below 200 meters of ocean water.

4. The deep ocean concentrate skin care kit of claim 1 wherein the deep ocean concentrate is obtained from a source of water below 500 meters of the ocean.

5. The deep ocean concentrate skin care kit according to claim 1, wherein the skin care formulation is one or more of a whitening product, a moisturizing product, a wrinkle removing product, a wound healing product, a medical or cosmetic introducing product.

6. The deep ocean concentrate skin care kit according to claim 5, wherein the wrinkle removing product is a collagen proliferation promoting product.

7. The deep ocean concentrate skin care kit of claim 1 wherein the deep ocean concentrate is a liquid or a powder.

8. Use of a deep ocean concentrate skin care kit for the preparation of a pharmaceutical composition for treating or caring skin, comprising administering the deep ocean concentrate skin care kit of claim 1 to the skin immediately after preparation.

9. The use of claim 8, wherein the deep ocean concentrate skin care kit provides quick access to the dermis layer and provides wrinkle reduction, skin whitening, and firmness.