CN109874789A - A method of using sustained release agent and carrier dual-sustained-release technology come slow release pest sex pheromone - Google Patents
A method of using sustained release agent and carrier dual-sustained-release technology come slow release pest sex pheromone Download PDFInfo
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- CN109874789A CN109874789A CN201910157159.7A CN201910157159A CN109874789A CN 109874789 A CN109874789 A CN 109874789A CN 201910157159 A CN201910157159 A CN 201910157159A CN 109874789 A CN109874789 A CN 109874789A
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Abstract
The invention discloses a kind of biological controls using sustained release agent and carrier dual-sustained-release technology come the method for slow release pest sex pheromone, for fruit tree pest insect oriental fruit months.Sex pheromone solution is obtained after sex pheromone of Grapholitha molesta is mixed with sustained release agent, then, selects release carrier of the plastic tube as sex pheromone, the isotropic element of oriental fruit months property can be obtained.The isotropic element of property of dual-sustained-release provided by the present invention can be realized the slow release of sex pheromone, it is short to solve the isotropic product lasting period of oriental fruit months property currently on the market, the release of pheromones component is too fast, and if pheromone release amount is too low, and the problems such as control efficiency is not achieved.
Description
Technical field
The present invention relates to the slow release method of sex pheromone of Grapholitha molesta, the biology for belonging to fruit tree pest insect oriental fruit months is anti-
Control field.
Background technique
Oriental fruit months has become the primary pest for endangering China main producing region Li Hetao, seriously affects fruit quality and pears
Fruit yield.For a long time, in agricultural production to the prevention and treatment of oriental fruit months still based on chemical pesticide, but its drug resistance is sent out
Exhibition quickly, makes control efficiency have a greatly reduced quality, meanwhile, the residue problem of chemical pesticide annoyings always peasant.In recent years, property is believed
It ceases plain technology and shows good application effect in terms of monitoring with prevention and control oriental fruit months.Insect sex pheromone is produced by insect
It is raw, outside releaser, it can lure or evoke the anisotropic insect progress movable chemical substance of mating of the same race.Technology that pheromones are isotropic is just
It is to confuse male pest in field adaptability female adult pheromones using special release, it is made to can not find mating object, thus dry
The mating behavior of male and female is disturbed and hindered, the population growth of pest is finally inhibited.Pheromones are isotropic product is particularly suitable for big
The crops of area contiguous plant, control efficiency is obvious, and saves labor cost and reduce labor intensity, is American-European-Japanese prosperity
The mainstream of national information element product.
It needs to keep certain density property information for a long time in prevention and treatment region using the isotropic technology prevention pest of sex pheromone
Plain component.But since sex pheromone has volatility, chemical component is relative complex, is difficult to be mass produced, cost compares
Height, therefore in practical application, slow release method must be utilized, to achieve the purpose that effectively to delay the rate of release of pheromones.It presses
The mode of action between sustained release agent and active material, slow release method are commonly divided into physics sustained release and chemical slow release two major classes.Object
Reason sustained release is usually by active material dissolution, disperses or be embedded in sustained release agent, hinders to make to play the release of active material
With.Chemical slow release refer between active material and sustained release agent with chemical bond is connected and formed delivery systme.Chemical slow release technology
Medicine, in terms of application it is very mature, and significant effect, but but application is compared in terms of sex pheromone
It is few.The unit raw medicine dosage of the isotropic element of oriental fruit months property is 240mg-500mg, 90 days lasting periods-on domestic and international market at present
150 days.The lasting period of the existing isotropic element product of property is shorter, sometimes needs to use twice in one season of growth of fruit tree, people
Work high expensive.And sex pheromone burst size is too low is unable to reach the problem of effectively preventing pest.If being excessively used property is believed
Breath element, although control efficiency is guaranteed, the cost of product is obviously higher, is unfavorable for promoting and applying.
Summary of the invention
The present invention provides a kind of dual-sustained-release technology of isotropic element of oriental fruit months property, can effectively achieve fruit tree pest insect pears
The biological control of Grapholita spp, the sex pheromone sustained release period is long, and isotropic excellent effect, can reach within the sustained release period
Excellent control efficiency.The technology can overcome the disadvantage that control efficiency is good in the prior art and the lasting period is short simultaneously, using this
The method of invention, not only the lasting period is long, but also control efficiency is good, and sex pheromone dosage is few, and the original of sex pheromone is greatly saved
Medicine cost.
The present invention provides a kind of isotropic element of sex pheromone of oriental fruit months by sustained release agent and carrier dual-sustained-release.It will
Sex pheromone of Grapholitha molesta component mixes according to a certain percentage, and sustained-release liquid is added and is made into pheromones solution, and will be described
Pheromones solution is added in release carrier, the property of can be obtained is isotropic element.The sex pheromone of Grapholitha molesta and sustained-release liquid
Weight ratio is 1:1-10.
The sex pheromone of Grapholitha molesta effective component is by mass percentage are as follows: Z-8- dodecylene -1- acetic acid esters
85.5-89.9%, E-8- dodecylene -1- acetic acid esters 6.9-8.5%, Z-8- dodecylene -1- alcohol 0.73-0.99%.
Wherein the sustained-release liquid is atoleine or commercially available edible oil.Commercially available edible oil is peanut oil, rapeseed oil, soybean
Oil, corn oil or olive oil.
Wherein the release carrier is plastic tube, including pvc tube, vinyl tube, polypropylene plastics
Pipe.The internal diameter of plastic tube is between 0.5 millimeter to 5 millimeters, and length is between 200-500 millimeters.
When specifically used oriental fruit months information disposition of the present invention is isotropic element, to occur in oriental fruit months early
Phase that is, before adult eclosion, starts to use in orchard, can reach good control efficiency.The isotropic element of oriental fruit months property
Application method it is simple and easy, can save labour turnover.
The isotropic pipe of oriental fruit months of the present invention is in orchard in application, the both ends of release carrier plastics pipe are closed
, pheromones solution will not be trickled down on fruit.Pheromones are isotropic, and pipe is done in orchard in application, being suspended on top fruit tree, is
It is uniformly placed according to every 16 square metres of isotropic pipes, i.e., hangs 40 isotropic pipes per acre.The lasting period of isotropic pipe was on 180 days left sides
The right side, and very high isotropic rate and control efficiency can be reached during it.
Compared with the prior art, the advantages of the present invention are as follows: the rate of release of pheromones component drops in dual-sustained-release technology
It is low, and kept for the time of effective dose long, made property is isotropic, and element can effectively interfere pest to mate in orchard, a life
Long season need to only use primary；In the also extended release time limit, Insect pest suppression effect is very significant, greatly reduces pears in orchard
The larva radix of Grapholita spp, and this will not develop drug resistance.The pheromones have many advantages, such as non-toxic, pollution-free.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below to the preferred embodiment of the present invention into
Row detailed description.
Embodiment 1:
Sex pheromone of Grapholitha molesta component (200mg) is encased in 2mL opening centrifuge tube, is placed at room temperature freely
Release records the variation of total weight every other week, and continuous observation surrounding calculates the burst size of sex pheromone of Grapholitha molesta, and
Pass through isotropic test statistics efficiency time and isotropic rate (being shown in Table 1).
Wherein: the sex pheromone of Grapholitha molesta is by mass percentage are as follows: Z-8- dodecylene -1- acetic acid esters
89.9%, E-8- dodecylene -1- acetic acid esters 6.9%, Z-8- dodecylene -1- alcohol 0.99%, remaining for other it is nonactive at
Point.
Wherein, isotropic experimental design:
The orchard for selecting a certain peach in certain city and pear tree mixing to plant, average 48 fruit trees of plantation per acre, the age of tree 9 years.Choosing
It selects 50 mu and is used as the isotropic treatment region of pheromones, in addition administer check plot as conventional for 10 mu.In isotropic area, by the small food of the pears
Equipment that heart worm is isotropic is arranged according to every 16 square metres one isotropic pipe, and the principle based on " periphery is close, and centre is dredged ", is guaranteed as far as possible
Uniform fold of the sex pheromone in orchard eliminates error caused by different location release unevenly.In check plot and isotropic area
Five sets of oriental fruit months trapper monitorings are inside randomly placed respectively, and 9 points of observation records lured and obtained adult every morning, and statistics lures borer population
Amount, to calculate isotropic rate.And in the pears peach maturity period, random searching orchard ripening fruits wormed fruit rate, calculate opposite prevention and treatment
Effect.
Interpretation of result:
Isotropic rate is the evaluation index that the isotropic technology of pheromones acts on oriental fruit months intraspecific interference, and calculation method is such as
Lower formula.Isotropic rate is higher, and the population density of oriental fruit months is lower in isotropic treatment region, illustrates pheromones to oriental fruit months
Control efficiency it is better.
Isotropic rate (%)=[(check plot induced moth number-treatment region induced moth number)/check plot induced moth number] × 100%
Embodiment 2:
It with corn oil is by weight that 1:1 is mixed by 1 sex pheromone of Grapholitha molesta component (200mg) of embodiment,
It is reloaded into open centrifuge tube.It is placed at room temperature for, records the variation of total weight every other week, it is small to calculate pears for continuous observation surrounding
The burst size of heart-eating worm sex pheromone, and by isotropic test statistics efficiency time and isotropic rate (being shown in Table 1),
Embodiment 3:
Other conditions are changed to 1 with embodiment 2, by sex pheromone of Grapholitha molesta component (200mg) and corn oil weight ratio:
3 are mixed, and are reloaded into open centrifuge tube.It is placed at room temperature for, records the variation of total weight, continuous observation four every other week
Week calculates the burst size of sex pheromone of Grapholitha molesta, and passes through isotropic test statistics efficiency time and isotropic rate (being shown in Table 1).
Embodiment 4:
Other conditions are changed to 1 with embodiment 2, by sex pheromone of Grapholitha molesta component (200mg) and corn oil weight ratio:
5 are mixed, and are reloaded into open centrifuge tube.It is placed at room temperature for, records the variation of total weight, continuous observation four every other week
Week calculates the burst size of sex pheromone of Grapholitha molesta, and passes through isotropic test statistics efficiency time and isotropic rate (being shown in Table 1).
Embodiment 5:
It with soybean oil is by weight that 1:1 is mixed by 1 sex pheromone of Grapholitha molesta component (200mg) of embodiment,
It is reloaded into 2mL opening centrifuge tube.It is placed at room temperature for, records the variation of total weight every other week, continuous observation surrounding calculates pears
The burst size of Grapholita spp sex pheromone, and pass through isotropic test statistics efficiency time and isotropic rate (being shown in Table 1)
Embodiment 6:
Other conditions are changed to 1 with embodiment 5, by sex pheromone of Grapholitha molesta component (200mg) and soybean oil weight ratio:
3 are mixed.It is placed at room temperature for, records the variation of total weight every other week, continuous observation surrounding calculates oriental fruit months property information
The burst size of element, and pass through isotropic test statistics efficiency time and isotropic rate (being shown in Table 1).
Embodiment 7:
Other conditions are changed to 1 with embodiment 5, by sex pheromone of Grapholitha molesta component (200mg) and soybean oil weight ratio:
5 are mixed, and are reloaded into 2mL opening centrifuge tube.It is placed at room temperature for, records the variation of total weight, continuous observation every other week
Surrounding calculates the burst size of sex pheromone of Grapholitha molesta, and (is shown in Table by isotropic test statistics efficiency time and isotropic rate
1)。
Embodiment 8:
With atoleine by weight it is that 1:1 is mixed by sex pheromone of Grapholitha molesta component (200mg), is reloaded into
Into 2mL opening centrifuge tube.It is placed at room temperature for, records the variation of total weight every other week, continuous observation surrounding calculates the small food heart of pears
The burst size of worm sex pheromone, and by isotropic test statistics efficiency time and with isotropic rate (being shown in Table 1).
Table 1: the burst size of sex pheromone of Grapholitha molesta in different sustained release agents
By obtaining table 1 after the observing and recording of lasting surrounding, it can be seen that pheromones raw medicine and the ratio of sustained release agent are 1:
When 3, rate of release is moderate.In view of soybean oil itself has kind of a special beany flavor, we have selected corn oil as sustained release agent.
Embodiment 9:
It is molten that pheromones are obtained after 1 oriental fruit months pheromones (200mg) of embodiment are mixed with corn oil by weight 1:3
Liquid is then encased in the polyethylene pipe that internal diameter is 0.5 millimeter, length is 250 millimeters, and is sealed with sealing machine, that is, makes
The isotropic pipe of oriental fruit months is obtained, is placed at room temperature for, records the variation of total weight every other week, continuous observation surrounding calculates the small food of pears
The burst size of heart worm sex pheromone, and pass through isotropic test statistics efficiency time and isotropic rate (being shown in Table 2).
Embodiment 10:
Other conditions are changed to 1.0 millimeters with embodiment 9, by vinyon bore, and prepared isotropic pipe room temperature is put
It sets, records the variation of total weight every other week, continuous observation surrounding calculates the burst size of sex pheromone of Grapholitha molesta, and leads to
Cross isotropic test statistics efficiency time and isotropic rate (being shown in Table 2).
Embodiment 11:
Other conditions are changed to 1.5 millimeters with embodiment 9, by vinyon bore, and prepared isotropic pipe room temperature is put
It sets, records the variation of total weight every other week, continuous observation surrounding calculates the burst size of sex pheromone of Grapholitha molesta, and leads to
Cross isotropic test statistics efficiency time and isotropic rate (being shown in Table 2).
Embodiment 12:
Other conditions are changed to pvc tube, prepared isotropic pipe room temperature with embodiment 9, by vinyl tube
It places, records the variation of total weight every other week, continuous observation surrounding calculates the burst size of sex pheromone of Grapholitha molesta, and
Pass through isotropic test statistics efficiency time and isotropic rate (being shown in Table 2).
Embodiment 13:
For other conditions with embodiment 9, it is 1.0 millimeters of polychlorostyrene that the vinyl tube that internal diameter is 0.5 millimeter, which is changed to internal diameter,
Vinyl tube, prepared isotropic pipe are placed at room temperature for, and record the variation of total weight every other week, and continuous observation surrounding calculates
The burst size of sex pheromone of Grapholitha molesta, and pass through isotropic test statistics efficiency time and isotropic rate (being shown in Table 2).
Embodiment 14:
For other conditions with embodiment 9, it is 1.5 millimeters of polychlorostyrene that the vinyl tube that internal diameter is 0.5 millimeter, which is changed to internal diameter,
Vinyl tube, prepared isotropic pipe are placed at room temperature for, and record the variation of total weight every other week, and continuous observation surrounding calculates
The burst size of sex pheromone of Grapholitha molesta, and pass through isotropic test statistics efficiency time and isotropic rate (being shown in Table 2).
Embodiment 15
Compared with embodiment 11, E-8- dodecylene -1- second is lacked in the sex pheromone of Grapholitha molesta effective component
Acid esters lacks component and is replaced with Z8- dodecylene -1- alcohol acetic ester, remaining component and condition, will be prepared with embodiment 10
Isotropic pipe is placed at room temperature for, and records the variation of total weight every other week, and continuous observation surrounding calculates sex pheromone of Grapholitha molesta
Burst size, and pass through isotropic test statistics efficiency time and isotropic rate (being shown in Table 2).
Embodiment 16
Compared with embodiment 11, Z-8- dodecylene -1- alcohol is lacked in the sex pheromone of Grapholitha molesta effective component,
Lack component to be replaced with Z8- dodecylene -1- alcohol acetic ester, remaining component and condition, will be prepared isotropic with embodiment 10
Pipe is placed at room temperature for, and records the variation of total weight every other week, and continuous observation surrounding calculates the release of sex pheromone of Grapholitha molesta
Amount, and pass through isotropic test statistics efficiency time and isotropic rate (being shown in Table 2).
Table 2: burst size of the sex pheromone of Grapholitha molesta in different tubing
By obtaining table 2 after the observing and recording of lasting surrounding, it has been found that pears in the polyethylene pipe that thickness of pipe wall is 1.0 millimeters
The rate of release of small sex pheromone is medium and fluctuation is smaller, therefore to select thickness of pipe wall be 1.0 millimeters, length is 250 millimeters poly-
Ethylene tube is used as the tubing of isotropic pipe.
Claims (8)
1. it is a kind of using sustained release agent and carrier dual-sustained-release technology come the method for slow release pest sex pheromone, feature exists
In: sex pheromone of Grapholitha molesta component and sustained-release liquid are made into pheromones solution by certain weight ratio, and by the pheromones
Solution is injected into release carrier, and the isotropic element of dual-sustained-release sex pheromone can be obtained.
2. according to claim 1 using sustained release agent and carrier dual-sustained-release technology come the side of slow release pest sex pheromone
Method, it is characterised in that: the sex pheromone of Grapholitha molesta effective component includes: Z8- dodecylene -1- alcohol acetic ester, E8- ten
Two carbene -1- alcohol acetic esters and Z8- dodecylene -1- alcohol.
3. according to claim 2 using sustained release agent and carrier dual-sustained-release technology come the side of slow release pest sex pheromone
Method, it is characterised in that: the sex pheromone of Grapholitha molesta effective component is by mass percentage are as follows: Z-8- dodecylene -1-
Acetic acid esters 85.5-89.9%, E-8- dodecylene -1- acetic acid esters 6.9-8.5%, Z-8- dodecylene -1- alcohol 0.73-
0.99%, remaining is other non-active ingredients.
4. according to claim 1 using sustained release agent and carrier dual-sustained-release technology come the side of slow release pest sex pheromone
Method, it is characterised in that: the weight ratio of the sex pheromone of Grapholitha molesta and sustained-release liquid is 1:1-10.
5. according to claim 1 using sustained release agent and carrier dual-sustained-release technology come the side of slow release pest sex pheromone
Method, it is characterised in that: the sustained-release liquid is atoleine or commercially available edible oil；Wherein, commercially available edible oil is peanut oil, vegetable seed
Oil, soybean oil, one of corn oil or olive oil.
6. using sustained release agent and carrier dual-sustained-release technology come slow release pest sex pheromone according to claims 1
Method, it is characterised in that: the release carrier includes pvc tube, vinyl tube, in pp pipeline
One or more.
7. using sustained release agent and carrier dual-sustained-release technology come slow release pest sex pheromone according to claims 6
Method, it is characterised in that: the internal diameter of the release carrier is between 0.5 millimeter to 5 millimeters, and length is between 200-500 millimeters.
8. according to any one of claims 1-7 it is described using sustained release agent and carrier dual-sustained-release technology come slow release pest property
The method of pheromones, it is characterised in that: the isotropic element of dual-sustained-release sex pheromone is in orchard in application, being suspended on fruit tree top
On trunk, placed according to every 16 square metres one isotropic pipe, the isotropic prevention and treatment lasting period was at 180 days.
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| CN201910157159.7A CN109874789A (en) | 2019-03-01 | 2019-03-01 | A method of using sustained release agent and carrier dual-sustained-release technology come slow release pest sex pheromone |
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| CN201910157159.7A CN109874789A (en) | 2019-03-01 | 2019-03-01 | A method of using sustained release agent and carrier dual-sustained-release technology come slow release pest sex pheromone |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111227363A (en) * | 2020-03-16 | 2020-06-05 | 肇庆市飞翔文化传播有限公司 | Mask with slow-release sterilization function |
| CN114145290A (en) * | 2021-06-02 | 2022-03-08 | 贵州省烟草科学研究院 | Efficient long-acting insect sex attractant core and manufacturing method thereof |
| CN115039742A (en) * | 2022-05-24 | 2022-09-13 | 江苏宁录科技股份有限公司 | Spodoptera frugiperda sex pheromone dual slow-release lure and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704210A (en) * | 2013-12-18 | 2014-04-09 | 浙江农林大学 | Oriental fruit moth imago attractant as well as lure and preparation method thereof |
| CN104798779A (en) * | 2015-03-17 | 2015-07-29 | 常州宁录生物科技有限公司 | Sex attractant for fruit tree pest Grapholitha molesta Busck |
| CN108967423A (en) * | 2018-09-12 | 2018-12-11 | 南京新安中绿生物科技有限公司 | Pest mating is interfered to attract sustained release preparation and the application of beneficial insect |
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2019
- 2019-03-01 CN CN201910157159.7A patent/CN109874789A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704210A (en) * | 2013-12-18 | 2014-04-09 | 浙江农林大学 | Oriental fruit moth imago attractant as well as lure and preparation method thereof |
| CN104798779A (en) * | 2015-03-17 | 2015-07-29 | 常州宁录生物科技有限公司 | Sex attractant for fruit tree pest Grapholitha molesta Busck |
| CN106900704A (en) * | 2015-03-17 | 2017-06-30 | 常州宁录生物科技有限公司 | A kind of property lure of fruit tree pest insect oriental fruit months |
| CN108967423A (en) * | 2018-09-12 | 2018-12-11 | 南京新安中绿生物科技有限公司 | Pest mating is interfered to attract sustained release preparation and the application of beneficial insect |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111227363A (en) * | 2020-03-16 | 2020-06-05 | 肇庆市飞翔文化传播有限公司 | Mask with slow-release sterilization function |
| CN114145290A (en) * | 2021-06-02 | 2022-03-08 | 贵州省烟草科学研究院 | Efficient long-acting insect sex attractant core and manufacturing method thereof |
| CN115039742A (en) * | 2022-05-24 | 2022-09-13 | 江苏宁录科技股份有限公司 | Spodoptera frugiperda sex pheromone dual slow-release lure and preparation method thereof |
| CN115039742B (en) * | 2022-05-24 | 2023-08-29 | 江苏宁录科技股份有限公司 | Spodoptera frugiperda sex pheromone dual slow-release lure core and preparation method thereof |
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