CN109846804A - A kind of method for extracting sheep embryo extract and the shin moisturizer containing sheep embryo extract - Google Patents
A kind of method for extracting sheep embryo extract and the shin moisturizer containing sheep embryo extract Download PDFInfo
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Abstract
The present invention provides a kind of shin moisturizer containing sheep embryo extract; it is characterized in that; it is made of sheep embryo extract and shin moisturizer matrix, the shin moisturizer matrix includes the component of following mass percent: EDTA-2Na 0.05%-0.1%, water-soluble jojoba oil 0.5%-1%; Vitamin C Ethyl Ether 0.5%-1%; niacinamide 0.1%-0.5%, Matrixyl -4 0.01%-0.02%, Argireline 0.01%-0.02%; hexylene glycol 0.5%-1%, remaining as distilled water.The invention also discloses the extracting methods of the sheep embryo extract.Shin moisturizer disclosed by the invention containing sheep embryo extract can mitigate wrinkle of skin, and delay skin aging, transdermal effect is significant, long shelf-life, it is non-stimulated to skin with more superior whitening effect, it is good using rear skin elasticity, cosmetology effect is good, wide adaptability, has very high promotional value.
Description
Technical field
The present invention relates to shin moisturizer technical field more particularly to a kind of method for extracting sheep embryo extract and containing sheep embryo extract
Shin moisturizer.
Background technique
Being constantly progressive with the abundant of material life and being gradually deteriorated for skin is increased with the age with social economy
Objective reality and people like to be beautiful and thirst for the eternal pursuit of young state, various skin care item are continuously developed out in recent years, its energy
Effectively improve the health and self-confident degree of user.The skin care item a great variety of commercial type at present, although playing certain
Skin care effect, still, since chemical synthesis substance is too many, some side effects of simultaneous and the obvious of pollution environment are lacked
Point limits its extensive use, is unable to satisfy people seeking beauty to the requirements at the higher level of skin care item.
With the exploitation of sheep embryo extract, existing some skin care item also use sheep embryo extract extracting solution, and sheep embryo extract is one
Kind has the small molecule of high bioactivity, and can quickly be absorbed by the body utilization, and use is safe, effect is obvious, it passes through enhancing
Immune function of human body adjusts human endocrine, accelerates the division of cell, repairs damaged cell, activates senile cell, keeps human body each
Cell and tissue reach younger state, and each function of human body is activated, and reach anti-aging truly, treat and prevent
The purpose of disease.However, the macromolecular substances in the sheep placenta extracted according to a conventional method are more, complicated component, anaphylaxis is high, activity
It is low, bioavailability is low.In addition, since sheep embryo extract is difficult in the skin protection cosmetics such as liquid state, cream kind to keep life for a long time
The activity of species extraction of substance, therefore existing product cannot get a desired effect.
At present declare on the market addition sheep placenta skin care item it is very much, but actual interpolation be mostly sheep placenta, be with
Goat Placenta is that the extraction of one of primary raw material or primary raw material is made.Goat Placenta is a kind of functional organ specifically changed, wherein containing
There are more human chorionic gonadtropin, estrogen, progestational hormone etc., and hormone can be removed in subtractive process without effective means
Or inactivation.The hormonal substance contained in sheep placenta part population can be adversely affected (children, pregnant woman and uterus,
Ovary, patient with mammary carcinoma)；And clearly by being included in of estrogenic chemicals in " cosmetics safety technical specification " (2015 editions)
Cosmetic disables component list, and the cosmetics for adding sheep placenta will face biggish regulation risk.
Application publication number is that 107898735 A of CN discloses a kind of compound sheep embryo extract shin moisturizer, utilizes sheep embryo
Element, propolis, madecassoside or brahmic acid and Chinese medical extract as raw material, cooperate chitosan, moisturizer,
Emollient, dextrin, guar gum, diglycol, carbonic acid dioctyl ester, Cetyl octanoate, deionized water carry out sheep embryo extract skin care
Deionized water is divided into three parts when preparation by the preparation of liquid, is separately added into other raw materials in three times and is carried out repeated ultrasonic high speed point
Dissipate finished product.The invention product preparation process method is simple, and prepared product has more superior whitening effect, to skin
Skin is non-stimulated, good using rear skin elasticity, and cosmetology effect is good.But this shin moisturizer complicated component, does not disclose sheep embryo
The extraction process of element is added with traditional Chinese medicine ingredients, Yi Yinqi allergy or rejection, and transdermal effect is bad, be unfavorable for activity at
The absorption divided.
Therefore, seek the method for extraction sheep embryo extract a kind of easy to operate, at low cost, and develop and a kind of can mitigate skin
Wrinkle, delay skin aging, transdermal effect is significant, long shelf-life, has more superior whitening effect, non-stimulated to skin,
Good using rear skin elasticity, the good shin moisturizer containing this sheep embryo extract of cosmetology effect accords with the demands of the market, and has wide
General market value and application prospect has very important significance to the development for promoting cosmetics industry.
Summary of the invention
The purpose of the present invention is overcome the deficiencies of the prior art and provide a kind of method for extracting sheep embryo extract and embryo containing sheep
The shin moisturizer of element, the extracting method of the sheep embryo extract is simple to operation, containing compared with comprehensive biological nutrition, work in the sheep embryo extract of extraction
Property substance, and hormone-content is extremely low；Shin moisturizer containing the sheep embryo extract can mitigate wrinkle of skin, delay skin aging, transdermal effect
Fruit is significant, long shelf-life, has more superior whitening effect, non-stimulated to skin, good using rear skin elasticity, improves looks
Effect is good, wide adaptability, has very high promotional value.
The present invention can be achieved through the following technical solutions:
A kind of shin moisturizer containing sheep embryo extract according to the present invention, which is characterized in that by sheep embryo extract and shin moisturizer
Matrix composition, the shin moisturizer matrix includes the component of following mass percent: EDTA-2Na 0.05%-0.1%, it is water-soluble suddenly
Bar oil 0.5%-1%, Vitamin C Ethyl Ether 0.5%-1%, niacinamide 0.1%-0.5%, -4 0.01%- of Matrixyl suddenly
0.02%, Argireline 0.01%-0.02%, hexylene glycol 0.5%-1%, remaining as distilled water.
Further, the sheep embryo extract, shin moisturizer matrix mass ratio be (2-5): 100.
Further, the preparation method of a kind of shin moisturizer containing sheep embryo extract, includes the following steps: EDTA-
2Na, water-soluble jojoba oil, Vitamin C Ethyl Ether, niacinamide, Matrixyl -4, Argireline, hexylene glycol, distilled water
It mixes in proportion, it is 10-15 minutes ultrasonic, sheep embryo extract is added afterwards, is uniformly mixed, obtains the shin moisturizer containing sheep embryo extract.
Further, the extracting method of the sheep embryo extract, includes the following steps:
Step S1: choosing the sheep embryo being pregnant 4-6 weeks, is tentatively rubbed after cleaning using Foods pulverizer, twists
Broken 1~2h rubs sheep embryo to paste, and the distilled water of handled 10 times of weight of sheep embryo of addition or more or physiology salt water logging
Bubble, makes cell suction moisture, is in swelling state, and cell can be crushed uniformly when subsequent grinding, and adequately protect wherein active material；
Step S2: the embryo's paste handled by step S1 is placed in 20~25h of freezing, sheep embryonic cell at -30 DEG C
Position is fixed rapidly, and cell rupture lacks cell liquid and is lost less, is freezed completely to sheep embryo, is further taken out the sheep embryo freezed in room temperature
Lower dissolution is ground using colloid mill afterwards；
Step S3: the distilled water of handled 1 times of weight of sheep embryo is added in the embryo's paste handled by step S2
It is centrifuged, solution temperature is -15~-10 DEG C or less in entire centrifugal process；
Step S4: the supernatant after taking centrifugation aseptically uses the ultrafiltration membrane of 100,000 molecular weight to be filtered, into
The preliminary removal of impurities of row；It also needs to be filtered using the ultrafiltration membrane of 10,000 molecular weight later, it at this time can be by macro-molecular protein in extract
It removes, and active material of the molecular weight less than 10,000, there are anti-aging effects, can be retained；It is extract obtained to be placed in -10 DEG C
Lower preservation, to inhibit the activity of wherein catabolic enzyme.
Preferably, the amount of water of distilled water described in step S1 is 10 times of handled sheep embryo weight.
Preferably, the distilled water is medical grade distilled water.
Preferably, in order to the time to be dissolved such as shorten, and the degree of crushing of sheep embryo is further provided for, it will be in step S2
The sheep embryo freezed is crushed 10~20min at normal temperature, and crushing energy at this time accelerates to dissolve and further rubs sheep embryo
To enhance its mobility, convenient for being ground in colloid mill；Being ground with colloid mill is also to carry out at normal temperature, and sheep embryo is further molten
Solution grinds be better than the crushing effect of cell for through the quick-frozen sheep embryo directly ground at this time；
Preferably, the colloid milling time is 1~2h.
It preferably, is abundant smudge cells, the freezing successively carried out in step S2, dissolution are crushed and are ground to one
Circulation abundant above-mentioned circulation 3 times, can reach microscopy without intact cell configuration, active material has been dissolved in steaming at this time after being repeated 3 times
In distilled water.
Further, a kind of sheep embryo extract is prepared using a kind of above-mentioned extracting method of sheep embryo extract.
Compared with the prior art, the invention has the benefit that
(1) a kind of method for extracting sheep embryo extract disclosed by the invention, this method is simple to operation, the sheep embryo extract of extraction
In containing compared with comprehensive biological nutrition, active material, and hormone-content is extremely low, has very high promotional value.
(2) a kind of shin moisturizer containing sheep embryo extract disclosed by the invention, the shin moisturizer for containing the sheep embryo extract can mitigate skin
Skin wrinkle, delay skin aging, transdermal effect is significant, long shelf-life, has more superior whitening effect, stingless to skin
Swash, good using rear skin elasticity, cosmetology effect is good, wide adaptability, and use is safe.
(3) a kind of shin moisturizer containing sheep embryo extract disclosed by the invention replaces sheep placenta to be applied to beauty with sheep embryo extract
Hold skin care item, skin can be made to obtain more fully nutrition and the drawback without hormone side effect.
(4) a kind of shin moisturizer containing sheep embryo extract disclosed by the invention, sheep embryo extract can promote cell division, activation aging
Cell plays and mitigates the beauty functions such as wrinkle, delay skin aging.Sheep embryo can be enhanced by being used cooperatively with shin moisturizer matrix
The skin degree of being absorbed and utilized of element, so that its effect is strengthened.
(5) a kind of shin moisturizer containing sheep embryo extract disclosed by the invention, shin moisturizer matrix each component synergistic effect, so that shield
Skin significant effect, transdermal characteristic is good, is more advantageous to skin absorption, can effectively cooperate with sheep embryo extract, extend the shelf life, so that skin care
Fluidity can be more stable.
Specific embodiment
In order that those skilled in the art will better understand the technical solution of the present invention, below with reference to embodiment to this hair
Bright product is described in further detail.
The drug and instrument being related in the embodiment of the present invention are commercially available.
Embodiment 1
A kind of shin moisturizer containing sheep embryo extract, which is characterized in that be made of sheep embryo extract and shin moisturizer matrix, the skin care
Liquid-based matter includes the component of following mass percent: EDTA-2Na 0.05%, water-soluble jojoba oil 0.5%, vitamin C ethyl
Ether 0.5%, niacinamide 0.1%, Matrixyl -4 0.01%, Argireline 0.01%, hexylene glycol 0.5% are remaining
For distilled water.
The sheep embryo extract, shin moisturizer matrix mass ratio be 2:100.
A kind of preparation method of the shin moisturizer containing sheep embryo extract, include the following steps: by EDTA-2Na, it is water-soluble flash
Bar oil, Vitamin C Ethyl Ether, niacinamide, Matrixyl -4, Argireline, hexylene glycol, distilled water mix in proportion,
Ultrasound 10 minutes, is added sheep embryo extract afterwards, is uniformly mixed, obtains the shin moisturizer containing sheep embryo extract.
The extracting method of the sheep embryo extract, includes the following steps:
Step S1: choosing the sheep embryo being pregnant 4 weeks, is tentatively rubbed after cleaning using Foods pulverizer, rubs
1h rubs sheep embryo to paste, and the distilled water immersion of handled 10 times of weight of sheep embryo is added, and cell is made to suction moisture,
In swelling state, cell can be crushed uniformly when subsequent grinding, and adequately protect wherein active material；
Step S2: the embryo's paste handled by step S1 being placed at -30 DEG C and freezes 20h, sheep embryonic cell position
Fixed rapidly, cell rupture lacks cell liquid and is lost less, freezes completely to sheep embryo, it is molten at room temperature to further take out the sheep embryo freezed
Solution is ground using colloid mill afterwards；
Step S3: the distilled water of handled 1 times of weight of sheep embryo is added in the embryo's paste handled by step S2
It is centrifuged, solution temperature is -15 DEG C or less in entire centrifugal process；
Step S4: the supernatant after taking centrifugation aseptically uses the ultrafiltration membrane of 100,000 molecular weight to be filtered, into
The preliminary removal of impurities of row；It also needs to be filtered using the ultrafiltration membrane of 10,000 molecular weight later, it at this time can be by macro-molecular protein in extract
It removes, and active material of the molecular weight less than 10,000, there are anti-aging effects, can be retained；It is extract obtained to be placed in -10 DEG C
Lower preservation, to inhibit the activity of wherein catabolic enzyme.
The distilled water is medical grade distilled water.
In order to the time to be dissolved such as shorten, and the degree of crushing of sheep embryo is further provided for, will frozen described in step S2
Sheep embryo firmly is crushed 10min at normal temperature, and crushing energy at this time accelerates to dissolve and rubs sheep embryo further to enhance its stream
Dynamic property, convenient for being ground in colloid mill；Being ground with colloid mill is also to carry out at normal temperature, and sheep embryo is further dissolved, and is ground at this time
It grinds and the crushing effect of cell is better than as through the quick-frozen sheep embryo directly ground；
The colloid milling time is 1h.
It is the freezing that is successively carried out in step S2, dissolution, broken and be ground to a circulation for abundant smudge cells, it fills
Divide above-mentioned circulation 3 times, can reach microscopy after being repeated 3 times without intact cell configuration, active material has been dissolved in distilled water at this time.
A kind of sheep embryo extract is prepared using a kind of above-mentioned extracting method of sheep embryo extract.
Embodiment 2
A kind of shin moisturizer containing sheep embryo extract, which is characterized in that be made of sheep embryo extract and shin moisturizer matrix, the skin care
Liquid-based matter includes the component of following mass percent: EDTA-2Na 0.06%, water-soluble jojoba oil 0.6%, vitamin C ethyl
Ether 0.6%, niacinamide 0.2%, Matrixyl -4 0.013%, Argireline 0.013%, hexylene glycol 0.6% remain
Remaining is distilled water.
The sheep embryo extract, shin moisturizer matrix mass ratio be 3:100.
A kind of preparation method of the shin moisturizer containing sheep embryo extract, include the following steps: by EDTA-2Na, it is water-soluble flash
Bar oil, Vitamin C Ethyl Ether, niacinamide, Matrixyl -4, Argireline, hexylene glycol, distilled water mix in proportion,
Ultrasound 12 minutes, is added sheep embryo extract afterwards, is uniformly mixed, obtains the shin moisturizer containing sheep embryo extract.
The extracting method of the sheep embryo extract, includes the following steps:
Step S1: choosing the sheep embryo being pregnant 5 weeks, is tentatively rubbed after cleaning using Foods pulverizer, rubs
1.2h rubs sheep embryo to paste, and the physiological saline that handled 12 times of weight of sheep embryo is added impregnates, and cell is made to suction water
Point, it is in swelling state, cell can be crushed uniformly when subsequent grinding, and adequately protect wherein active material；
Step S2: the embryo's paste handled by step S1 being placed at -30 DEG C and freezes 22h, sheep embryonic cell position
Fixed rapidly, cell rupture lacks cell liquid and is lost less, freezes completely to sheep embryo, it is molten at room temperature to further take out the sheep embryo freezed
Solution is ground using colloid mill afterwards；
Step S3: the distilled water of handled 1 times of weight of sheep embryo is added in the embryo's paste handled by step S2
It is centrifuged, solution temperature is -13 DEG C or less in entire centrifugal process；
Step S4: the supernatant after taking centrifugation aseptically uses the ultrafiltration membrane of 100,000 molecular weight to be filtered, into
The preliminary removal of impurities of row；It also needs to be filtered using the ultrafiltration membrane of 10,000 molecular weight later, it at this time can be by macro-molecular protein in extract
It removes, and active material of the molecular weight less than 10,000, there are anti-aging effects, can be retained；It is extract obtained to be placed in -10 DEG C
Lower preservation, to inhibit the activity of wherein catabolic enzyme.
In order to the time to be dissolved such as shorten, and the degree of crushing of sheep embryo is further provided for, will frozen described in step S2
Sheep embryo firmly is crushed 13min at normal temperature, and crushing energy at this time accelerates to dissolve and rubs sheep embryo further to enhance its stream
Dynamic property, convenient for being ground in colloid mill；Being ground with colloid mill is also to carry out at normal temperature, and sheep embryo is further dissolved, and is ground at this time
It grinds and the crushing effect of cell is better than as through the quick-frozen sheep embryo directly ground；
The colloid milling time is 1.2h.
It is the freezing that is successively carried out in step S2, dissolution, broken and be ground to a circulation for abundant smudge cells, it fills
Divide above-mentioned circulation 3 times, can reach microscopy after being repeated 3 times without intact cell configuration, active material has been dissolved in distilled water at this time.
A kind of sheep embryo extract is prepared using a kind of above-mentioned extracting method of sheep embryo extract.
Embodiment 3
A kind of shin moisturizer containing sheep embryo extract, which is characterized in that be made of sheep embryo extract and shin moisturizer matrix, the skin care
Liquid-based matter includes the component of following mass percent: EDTA-2Na 0.08%, water-soluble jojoba oil 0.8%, vitamin C ethyl
Ether 0.7%, niacinamide 0.3%, Matrixyl -4 0.015%, Argireline 0.015%, hexylene glycol 0.8% remain
Remaining is distilled water.
The sheep embryo extract, shin moisturizer matrix mass ratio be 4:100.
A kind of preparation method of the shin moisturizer containing sheep embryo extract, include the following steps: by EDTA-2Na, it is water-soluble flash
Bar oil, Vitamin C Ethyl Ether, niacinamide, Matrixyl -4, Argireline, hexylene glycol, distilled water mix in proportion,
Ultrasound 13 minutes, is added sheep embryo extract afterwards, is uniformly mixed, obtains the shin moisturizer containing sheep embryo extract.
The extracting method of the sheep embryo extract, includes the following steps:
Step S1: choosing the sheep embryo being pregnant 6 weeks, is tentatively rubbed after cleaning using Foods pulverizer, rubs
1.5h rubs sheep embryo to paste, and the physiological saline that handled 15 times of weight of sheep embryo is added impregnates, and cell is made to suction water
Point, it is in swelling state, cell can be crushed uniformly when subsequent grinding, and adequately protect wherein active material；
Step S2: the embryo's paste handled by step S1 being placed at -30 DEG C and freezes 23h, sheep embryonic cell position
Fixed rapidly, cell rupture lacks cell liquid and is lost less, freezes completely to sheep embryo, it is molten at room temperature to further take out the sheep embryo freezed
Solution is ground using colloid mill afterwards；
Step S3: the distilled water of handled 1 times of weight of sheep embryo is added in the embryo's paste handled by step S2
It is centrifuged, solution temperature is -12 DEG C or less in entire centrifugal process；
Step S4: the supernatant after taking centrifugation aseptically uses the ultrafiltration membrane of 100,000 molecular weight to be filtered, into
The preliminary removal of impurities of row；It also needs to be filtered using the ultrafiltration membrane of 10,000 molecular weight later, it at this time can be by macro-molecular protein in extract
It removes, and active material of the molecular weight less than 10,000, there are anti-aging effects, can be retained；It is extract obtained to be placed in -10 DEG C
Lower preservation, to inhibit the activity of wherein catabolic enzyme.
In order to the time to be dissolved such as shorten, and the degree of crushing of sheep embryo is further provided for, will frozen described in step S2
Sheep embryo firmly is crushed 15min at normal temperature, and crushing energy at this time accelerates to dissolve and rubs sheep embryo further to enhance its stream
Dynamic property, convenient for being ground in colloid mill；Being ground with colloid mill is also to carry out at normal temperature, and sheep embryo is further dissolved, and is ground at this time
It grinds and the crushing effect of cell is better than as through the quick-frozen sheep embryo directly ground；
The colloid milling time is 1.5h.
It is the freezing that is successively carried out in step S2, dissolution, broken and be ground to a circulation for abundant smudge cells, it fills
Divide above-mentioned circulation 3 times, can reach microscopy after being repeated 3 times without intact cell configuration, active material has been dissolved in distilled water at this time.
A kind of sheep embryo extract is prepared using a kind of above-mentioned extracting method of sheep embryo extract.
Embodiment 4
A kind of shin moisturizer containing sheep embryo extract, which is characterized in that be made of sheep embryo extract and shin moisturizer matrix, the skin care
Liquid-based matter includes the component of following mass percent: EDTA-2Na 0.09%, water-soluble jojoba oil 0.9%, vitamin C ethyl
Ether 0.9%, niacinamide 0.45%, Matrixyl -4 0.018%, Argireline 0.019%, hexylene glycol 0.9% remain
Remaining is distilled water.
The sheep embryo extract, shin moisturizer matrix mass ratio be 4:100.
A kind of preparation method of the shin moisturizer containing sheep embryo extract, include the following steps: by EDTA-2Na, it is water-soluble flash
Bar oil, Vitamin C Ethyl Ether, niacinamide, Matrixyl -4, Argireline, hexylene glycol, distilled water mix in proportion,
Ultrasound 14 minutes, is added sheep embryo extract afterwards, is uniformly mixed, obtains the shin moisturizer containing sheep embryo extract.
The extracting method of the sheep embryo extract, includes the following steps:
Step S1: choosing the sheep embryo being pregnant 5 weeks, is tentatively rubbed after cleaning using Foods pulverizer, rubs
1.8h rubs sheep embryo to paste, and handled sheep embryo 10 times of distilled water immersions of weight are added, and cell is made to suction moisture,
In swelling state, cell can be crushed uniformly when subsequent grinding, and adequately protect wherein active material；
Step S2: the embryo's paste handled by step S1 being placed at -30 DEG C and is freezed for 24 hours, sheep embryonic cell position
Fixed rapidly, cell rupture lacks cell liquid and is lost less, freezes completely to sheep embryo, it is molten at room temperature to further take out the sheep embryo freezed
Solution is ground using colloid mill afterwards；
Step S3: the distilled water of handled 1 times of weight of sheep embryo is added in the embryo's paste handled by step S2
It is centrifuged, solution temperature is -11 DEG C or less in entire centrifugal process；
Step S4: the supernatant after taking centrifugation aseptically uses the ultrafiltration membrane of 100,000 molecular weight to be filtered, into
The preliminary removal of impurities of row；It also needs to be filtered using the ultrafiltration membrane of 10,000 molecular weight later, it at this time can be by macro-molecular protein in extract
It removes, and active material of the molecular weight less than 10,000, there are anti-aging effects, can be retained；It is extract obtained to be placed in -10 DEG C
Lower preservation, to inhibit the activity of wherein catabolic enzyme.
The distilled water is medical grade distilled water.
In order to the time to be dissolved such as shorten, and the degree of crushing of sheep embryo is further provided for, will frozen described in step S2
Sheep embryo firmly is crushed 18min at normal temperature, and crushing energy at this time accelerates to dissolve and rubs sheep embryo further to enhance its stream
Dynamic property, convenient for being ground in colloid mill；Being ground with colloid mill is also to carry out at normal temperature, and sheep embryo is further dissolved, and is ground at this time
It grinds and the crushing effect of cell is better than as through the quick-frozen sheep embryo directly ground；
The colloid milling time is 1.8h.
It is the freezing that is successively carried out in step S2, dissolution, broken and be ground to a circulation for abundant smudge cells, it fills
Divide above-mentioned circulation 3 times, can reach microscopy after being repeated 3 times without intact cell configuration, active material has been dissolved in distilled water at this time.
A kind of sheep embryo extract is prepared using a kind of above-mentioned extracting method of sheep embryo extract.
Embodiment 5
A kind of shin moisturizer containing sheep embryo extract, which is characterized in that be made of sheep embryo extract and shin moisturizer matrix, the skin care
Liquid-based matter includes the component of following mass percent: EDTA-2Na 0.1%, water-soluble jojoba oil 1%, Vitamin C Ethyl Ether
1%, niacinamide 0.5%, Matrixyl -4 0.02%, Argireline 0.02%, hexylene glycol 1%, remaining as distillation
Water.
The sheep embryo extract, shin moisturizer matrix mass ratio be 5:100.
A kind of preparation method of the shin moisturizer containing sheep embryo extract, include the following steps: by EDTA-2Na, it is water-soluble flash
Bar oil, Vitamin C Ethyl Ether, niacinamide, Matrixyl -4, Argireline, hexylene glycol, distilled water mix in proportion,
Ultrasound 15 minutes, is added sheep embryo extract afterwards, is uniformly mixed, obtains the shin moisturizer containing sheep embryo extract.
The extracting method of the sheep embryo extract, includes the following steps:
Step S1: choosing the sheep embryo being pregnant 6 weeks, is tentatively rubbed after cleaning using Foods pulverizer, rubs
2h rubs sheep embryo to paste, and distilled water or the physiological saline immersion of handled 10 times of weight of sheep embryo is added, and makes cell
Moisture is suctioned, swelling state is in, cell can be crushed uniformly when subsequent grinding, and adequately protect wherein active material；
Step S2: the embryo's paste handled by step S1 being placed at -30 DEG C and freezes 25h, sheep embryonic cell position
Fixed rapidly, cell rupture lacks cell liquid and is lost less, freezes completely to sheep embryo, it is molten at room temperature to further take out the sheep embryo freezed
Solution is ground using colloid mill afterwards；
Step S3: the distilled water of handled 1 times of weight of sheep embryo is added in the embryo's paste handled by step S2
It is centrifuged, solution temperature is -10 DEG C or less in entire centrifugal process；
Step S4: the supernatant after taking centrifugation aseptically uses the ultrafiltration membrane of 100,000 molecular weight to be filtered, into
The preliminary removal of impurities of row；It also needs to be filtered using the ultrafiltration membrane of 10,000 molecular weight later, it at this time can be by macro-molecular protein in extract
It removes, and active material of the molecular weight less than 10,000, there are anti-aging effects, can be retained；It is extract obtained to be placed in -10 DEG C
Lower preservation, to inhibit the activity of wherein catabolic enzyme.
The distilled water is medical grade distilled water.
In order to the time to be dissolved such as shorten, and the degree of crushing of sheep embryo is further provided for, will frozen described in step S2
Sheep embryo firmly is crushed 20min at normal temperature, and crushing energy at this time accelerates to dissolve and rubs sheep embryo further to enhance its stream
Dynamic property, convenient for being ground in colloid mill；Being ground with colloid mill is also to carry out at normal temperature, and sheep embryo is further dissolved, and is ground at this time
It grinds and the crushing effect of cell is better than as through the quick-frozen sheep embryo directly ground；
The colloid milling time is 2h.
It is the freezing that is successively carried out in step S2, dissolution, broken and be ground to a circulation for abundant smudge cells, it fills
Divide above-mentioned circulation 3 times, can reach microscopy after being repeated 3 times without intact cell configuration, active material has been dissolved in distilled water at this time.
A kind of sheep embryo extract is prepared using a kind of above-mentioned extracting method of sheep embryo extract.
Comparative example
The present invention provides a kind of compound sheep embryo extract shin moisturizer, implements according to Chinese invention patent CN 107898735A
The formula and preparation method of example one are prepared.
The sheep embryo extract shin moisturizer skin effect of embodiment 1-5 and comparative example is verified, verification method is as follows: random
The women that 70 ages are 35~45 years old is chosen, 70 faces grow obvious wrinkle.70 people are randomly divided into 7 groups, respectively A,
B, C, D, E, F, G group, every group of 10 people, everyone uses sheep embryo extract shin moisturizer 1 time of embodiment 1 A group daily, everyone is daily for B group
Using sheep embryo extract shin moisturizer 1 time of embodiment 2, everyone uses sheep embryo extract shin moisturizer 1 time of embodiment 3 to C group daily, D group
Everyone uses sheep embryo extract shin moisturizer 1 time of embodiment 4 daily, E group everyone use the sheep embryo extract shin moisturizer of embodiment 5 daily
1 time, everyone uses the shin moisturizer matrix in embodiment 11 time F group daily, G group everyone use the sheep embryo extract of comparative example to protect daily
It skin liquid 1 time, is used continuously 2 weeks.
Test front and back carries out comparison scoring of taking pictures to test personnel's facial wrinkles, then carries out statistics calculating to using effect,
The results are shown in Table 1.Standards of grading are as follows:
| Test result | Scoring |
| Main wrinkle length reduces 30% or more | 3 points |
| Main wrinkle length reduces 20%~30% | 2 points |
| Main wrinkle length reduces 10%~20% | 1 point |
| Wrinkle state is without substantially changeing | 0 point |
Wherein, effective is scoring at 3 points, be effectively scoring is 1-2 point, and invalid is 0 point.
Table 1
| Group | A | B | C | D | E | F | G |
| Effective (people) | 3 | 4 | 5 | 6 | 8 | 0 | 0 |
| Effectively (people) | 6 | 6 | 5 | 4 | 2 | 8 | 6 |
| (people) in vain | 1 | 0 | 0 | 0 | 0 | 2 | 4 |
| Overall efficiency (%) | 90 | 100 | 100 | 100 | 100 | 80 | 60 |
As seen from Table 1, using the test personnel of sheep embryo extract shin moisturizer of the present invention, facial wrinkles improve situation and are substantially better than
Shin moisturizer matrix control group illustrates that sheep embryo extract shin moisturizer provided by the invention can effectively desalinate wrinkle, enhances skin elasticity, prolong
Slow skin aging.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (9)
1. a kind of shin moisturizer containing sheep embryo extract, which is characterized in that be made of sheep embryo extract and shin moisturizer matrix, the shin moisturizer
Matrix includes the component of following mass percent: EDTA-2Na 0.05%-0.1%, water-soluble jojoba oil 0.5%-1%, dimension life
Plain C ethylether 0.5%-1%, niacinamide 0.1%-0.5%, -4 0.01%-0.02% of Matrixyl, Argireline
0.01%-0.02%, hexylene glycol 0.5%-1%, remaining as distilled water.
2. a kind of shin moisturizer containing sheep embryo extract according to claim 1, which is characterized in that the sheep embryo extract, skin care
The mass ratio of liquid-based matter is (2-5): 100.
3. a kind of shin moisturizer containing sheep embryo extract according to claim 1, which is characterized in that described a kind of containing sheep embryo extract
Shin moisturizer preparation method, include the following steps: by EDTA-2Na, water-soluble jojoba oil, Vitamin C Ethyl Ether, niacinamide,
Matrixyl -4, Argireline, hexylene glycol, distilled water mix in proportion, 10-15 minutes ultrasonic, rear that sheep embryo is added
Element is uniformly mixed, obtains the shin moisturizer containing sheep embryo extract.
4. a kind of shin moisturizer containing sheep embryo extract according to claim 1, which is characterized in that the extraction of the sheep embryo extract
Method includes the following steps:
Step S1: choosing the sheep embryo being pregnant 4-6 weeks, is tentatively rubbed after cleaning using Foods pulverizer, and rubbing 1~
2h rubs sheep embryo to paste, and the distilled water of handled 10 times of weight of sheep embryo of addition or more or physiological saline impregnate, and make
Cell suctions moisture, is in swelling state, and cell can be crushed uniformly when subsequent grinding, and adequately protect wherein active material；
Step S2: the embryo's paste handled by step S1 is placed in 20~25h of freezing, sheep embryonic cell position at -30 DEG C
Fixed rapidly, cell rupture lacks cell liquid and is lost less, freezes completely to sheep embryo, it is molten at room temperature to further take out the sheep embryo freezed
Solution is ground using colloid mill afterwards；
Step S3: being added the distilled water centrifugation of handled 1 times of weight of sheep embryo in the embryo's paste handled by step S2,
Solution temperature is -15~-10 DEG C or less in entire centrifugal process；
Step S4: the supernatant after taking centrifugation aseptically uses the ultrafiltration membrane of 100,000 molecular weight to be filtered, and carries out just
Step removal of impurities；It also needs to be filtered using the ultrafiltration membrane of 10,000 molecular weight later, macro-molecular protein in extract can be removed at this time
It goes, and active material of the molecular weight less than 10,000, there are anti-aging effects, can be retained；It is extract obtained to be placed at -10 DEG C
It saves, to inhibit the activity of wherein catabolic enzyme.
5. a kind of shin moisturizer containing sheep embryo extract according to claim 4, which is characterized in that distilled water described in step S1
Amount of water be 10 times of handled sheep embryo weight.
6. a kind of shin moisturizer containing sheep embryo extract according to claim 4, which is characterized in that the distilled water is medical grade
Distilled water.
7. a kind of shin moisturizer containing sheep embryo extract according to claim 4, which is characterized in that freezed described in step S2
Sheep embryo is crushed 10~20min at normal temperature, and crushing energy at this time accelerates to dissolve and rubs sheep embryo further to enhance its stream
Dynamic property, convenient for being ground in colloid mill；Being ground with colloid mill is also to carry out at normal temperature, and sheep embryo is further dissolved.
8. a kind of shin moisturizer containing sheep embryo extract according to claim 4, which is characterized in that the colloid milling time
For 1~2h.
9. a kind of shin moisturizer containing sheep embryo extract according to claim 4, which is characterized in that with successively in the step S2
The freezing of progress, is crushed and is ground to a circulation at dissolution, abundant above-mentioned circulation 3 times, can reach microscopy after being repeated 3 times without complete
Whole eucaryotic cell structure, active material has been dissolved in distilled water at this time.
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| US11571378B2 (en) | 2021-01-22 | 2023-02-07 | The Procter & Gamble Company | Skin care composition and method of using the same |
| US11771637B2 (en) | 2021-01-22 | 2023-10-03 | The Procter & Gamble Company | Skin care composition and method of using the same |
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