CN109832607A - A kind of preparation method of coordinating intestines and stomach function ferment - Google Patents
A kind of preparation method of coordinating intestines and stomach function ferment Download PDFInfo
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Abstract
The present invention provides a kind of preparation method of coordinating intestines and stomach function ferment, by grape fruit, dregs of rapeseed cake, tea-cake dregs, scutellariae,radix, malt are prepared by compound bacteria fermentation, enzyme activity content of material prepared by the present invention is high, while improving functions of intestines and stomach, moreover it is possible to improve the immunity of the human body and be metabolized, there is good economic benefit.Preparation process of the invention is simple, and the period is short, and ferment nutritive value is high.
Description
Technical field
The invention belongs to health food preparation technical field, in particular to a kind of preparation side of coordinating intestines and stomach function ferment
Method.
Background technique
With the improvement of living standards, the factors such as life stress increase, environmental pollution cause human endogenous's property enzyme to reduce, food
Product finishing destroys exogenous enzymes again, and unreasonable dietary structure causes nutrient to lack, so that functions of intestines and stomach disorder,
Gastrointestinal microbiological group's confusion causes the incidence of the alimentary canals such as enterogastritis, colitis and blood chronic disease higher and higher, body
Easily occur that enzyme is unbalance or declines, unbalanced nutrition and sub-health state is presented.Enzyme food can effectively adjust body enzyme
System and nutrient balance make human body keep fit state, and therefore, ferment is increasingly approved and pushed away as a kind of health-preserving food
It is high.It is prevailing on Japan, the U.S., Taiwan and other places at present, and it is relatively slow in China's Mainland development, and most enzyme foods are all
From import, the market price is expensive, and carrying out scientific research to preparation method has huge value.
Patent of invention CN201210317614.3 longan ferment capable of soothing nerves and invigorating stomach processing method and formula, take processing fresh
Spontaneous fermentation forms a variety of fruits and vegetables such as fruit (especially pineapple), dried longan (or dried longan pulp), plant beans together, is extracted it
In fruits and vegetables essence, the plant water-solubles fiber such as product fruit rich in, sufficient vitamin needed by human, amino
The microelements such as acid, minerals；The design of the invention simultaneously is rationally matching for main effect using diversification comprehensive enzyme, probiotics
Side has fall fire, ease constipation, defaecation, improvement sleep and other effects, (first conserves enteron aisle and bacterium phase) based on gastrointestinal system, and be aided with
Functional food materials promote intestines peristalsis and free stool.
A kind of multi-functional ferment and preparation method thereof for purifying stomach of patent of invention CN201610591537.9, raw material packet
It includes: Maca, pineapple, car li, blackcurrant, wax-apple, lemon, Kiwi berry, butter fruit, grape fruit, manaca, chlorella powder, red
Beans, mung bean, soya bean, corn, semen coicis, Semen Lablab Album, Radix Angelicae Sinensis, motherwort, argy wormwood, rhizoma polygonati and witloof, more function made from the invention
Energy ferment can purify gastrointestinal environment, remove enteron aisle garbage toxins, decompose harmful carcinogen and superabundant fats, play stable enteron aisle
PH value and the function of activating intestines peristalsis.
In the prior art, the ferment of coordinating intestines and stomach is all to carry out fermentation with a variety of medicinal material combination fruit-vegetable foods to be prepared,
Raw material dosage is big, and nutritional ingredient is more and complicated, and specific aim is not strong, simultaneously as the multifarious limitation of raw material leads to fermentation period
It is long, nutrient loss.
Summary of the invention
For defect existing for prior art preparation coordinating intestines and stomach ferment, the present invention provides a kind of system of coordinating intestines and stomach ferment
Preparation Method.
The present invention is realized by following technical side:
A kind of preparation method of coordinating intestines and stomach function ferment, is prepared by the following steps and obtains:
(1) by ratio of weight and the number of copies, 15 ~ 20 parts of grape fruit are taken, peeling separates fruit and pericarp, then fruit squeezed the juice, institute
It obtains and retains pulp in fruit juice, while pericarp solarization dry doubling is crushed into powder end, obtain grape grapefruit juice and grapefruit peel powder；Take wheat
10 ~ 20 parts of bud, add water enchroachment (invasion) bubble 20 ~ for 24 hours afterwards take out smash to pieces, be added saccharomycete ferment, malt fermentation liquid is obtained, then by wheat
Bud fermentation liquid is uniformly mixed with grape grapefruit juice, bentonite is added by the 6 ~ 8% of total liquid quality, with vinegar acid for adjusting pH to 1 ~ 2, room
After the lower 1 ~ 3h of stirring and leaching of temperature, centrifugation removal bentonite precipitating obtains extracting solution；
(2) by ratio of weight and the number of copies, 15 ~ 20 parts of scutellariae,radix are taken, the distillations that granularity is 8 ~ 10 times of amounts of addition after 40 ~ 50 mesh are crushed to
Water decocts 1.5 ~ 2h and obtains radix scutellariae medical fluid, and after radix scutellariae medical fluid is cooled to 40 ~ 60 DEG C, 12 ~ 15 points are centrifuged under 2000r/min
Clock takes supernatant to get radix scutellariae extracting solution is arrived；By radix scutellariae extracting solution mass ratio be added 1.0% peptone, 0.05% sodium chloride,
Adjusting pH is 7.0 ~ 8.6, and high pressure sterilization simultaneously is cooled to room temperature to obtain radix scutellariae fermentation liquid I, is then inoculated in radix scutellariae fermentation liquid I multiple
Combined bacteria I obtains radix scutellariae fermentation liquid II in 45 ~ 55 DEG C, 100 ~ 200r/min shaking table 4 ~ 6d of top fermentation；
By I volume basis of radix scutellariae fermentation liquid, the compound bacteria I be 4 ~ 6% Bacillus nattoBacillus nattoBacterium solution and 3 ~ 5% mouse
Lee's sugar lactobacillusLactobacillus rhamnosusBacterium solution combination；
(3) by ratio of weight and the number of copies, 10 ~ 20 parts of dregs of rapeseed cake, 10 ~ 20 parts of tea-cake dregs are taken, is uniformly mixed after being crushed to 50 ~ 100 mesh,
Obtain grouts powder；Grapefruit peel powder, grouts powder are uniformly mixed, the water of 10 ~ 20 times of amounts is added, high pressure is gone out after mixing
Bacterium obtains fermentation liquid III, and after its cooling, inoculation compound bacteria II is fermented at 35 ~ 40 DEG C；
By III volume basis of fermentation liquid, the compound bacteria II be 4 ~ 6% bacillus subtilisBacillus subtilisBacterium solution
With 2 ~ 4% aspergillus nigerAspergillus nigerBacterium solution combination；
(4) after fermentation liquid III ferments 6 ~ 7 days, extracting solution and radix scutellariae fermentation liquid II is added, at the same be added III volume ratio 5 of fermentation liquid ~
10% chitosan solution is uniformly mixed after continuing fermentation 6 ~ 7 days, by filtering fermentation liquor, obtains clarified solution, by clarified solution in 70 ~
The ferment of coordinating intestines and stomach can be obtained in 30 ~ 50min of high pressure sterilization at 80 DEG C after cooling.
It adsorbs excessive extract on bentonite in order to prevent in step (1), first time can be centrifuged to gained precipitating and added
Enter 2 times of amount distilled water to be washed, then be centrifugated removal precipitating, by gained filtrate and is centrifugated gained filtrate for the first time and closes
And；
As a further improvement of the present invention, step (1) the grape fruit pericarp is dried to that water content is 10 ~ 20%, is crushed to 15
~ 20 mesh.
The leaching rate of particle size influences folic acid, can completely in 15 ~ 20 mesh folic acid and maximization is extracted leaching.
As a further improvement of the present invention, the water temperature that step (1) described malt is soaked in water is 15 ~ 20 DEG C, specific to send out
Fermenting process is as follows: its 1 ~ 1.5 times water of quality and 1 ~ 1.2% sodium chloride is added in the malt after smashing to pieces, is inoculated with 3% saccharomycete
Ferment 6h at 35 DEG C, obtains malt fermentation liquid.
Phytic acid in malt can be gradually decreased with the growth of malt, and phytic acid activity reduces at low temperature, malt growth by
To inhibition, the consumption of phytic acid is reduced；Yeast fermentation changes the structure and metabolism of malt, improves the yield of phytic acid.
Contain tetrahydrofolate components in grape fruit, promote stomach amino acid and glycometabolism, the synthesis of human body choline is improved, to promote
Fat metabolism, folic acid can also promote dregs of rapeseed cake and cake of camellia oleifera seeds to decompose release phytic acid and Tea Saponin；Contain in malt and rapeseed dregs
There is phytic acid abundant, thallus fermentation efficiency can be promoted, to improve ferment content；Phosphorus after hydrolysis of phytic acid can improve tunning
The vigor of enzyme, to improve the nutritive value of ferment；Natural montmorillonite can protein impurities and color effectively in adsorbing and extracting liquid
The bentonite of element, dosage 6g/10ml is maximum to the adsorption capacity of protein impurities and pigment, improves the pure of malt phytic acid
Degree.
Contain Tea Saponin in tea-cake dregs, there is anti-inflammatory analgesic, stomach invigorating and other effects, while tea-cake dregs and rapeseed dregs are microorganisms
The good sources of carbon and nitrogen source of fermentation；
As a further improvement of the present invention, swelling soil granularity described in step (1) is 100 ~ 120 mesh.
As a further improvement of the present invention, step (2) compound bacteria I is the Bacillus natto for being 12 ~ 14h by kind of ageBacillus nattoIt is 14 ~ 16h Lactobacillus rhamnosus with kind of ageLactobacillus rhamnosusCombination.
Baicalein in radix scutellariae fermentation liquid can improve malt phytic acid dissolution rate；Kind age is 12 ~ 14h Bacillus natto in fermentation process
In can generate the multiple active enzymes such as Nattokinase, superoxide dismutase, glucuronic acid key that can effectively in hydrolytic glycone,
By in radix scutellariae scutelloside and wogonoside be converted to baicalein and wogonin；The rhamnose lactic acid bacteria that kind age is 14 ~ 16h sends out
Ferment secretes rhamnolipid ability maximum, can improve the bacterium vigor of Bacillus natto, enhance the metabolism of Bacillus natto, to promote scutelloside
It is converted to baicalein and wogonin with wogonoside, reduces overall fermentation time；Lactobacillus rhamnosus is one of human flora,
The activity of various enzymes and the quantity of Bacillus natto bacterium in reaction system can be improved, the balance for adjusting flora is played, improves body environment
Effect.
As a further improvement of the present invention, step (3) compound bacteria II is to plant the bacillus subtilis that age is 12 ~ 14h
BacteriumBacillus subtilisThe aspergillus niger of sumAspergillus nigerCombination.
Mixed fermentation tea-cake dregs and rapeseed dregs effect are fermented better than single bacterium, using enter logarithmic growth phase (kind age 12 ~
Bacillus subtilis and aspergillus niger 14h) ferments to tea-cake dregs and rapeseed dregs, can maximize and decompose to it, generates
Various enzymes and Tea Saponin and phytic acid.
As a further improvement of the present invention, deacetylating degree of chitosan described in step (4) is 50 ~ 60%.
Chitosan has good enzyme adsorption capacity, can adsorb the enzyme in fermentation liquid, improves fermentation enzyme amount；Chitosan
Suction-operated fermentation liquid can also be made to clarify, reduce the cost of downstream engineering；The chitosan water solubility of deacetylation 50 ~ 60% is most
It is good, it can effectively be dissolved in fermentation liquid；
Beneficial effects of the present invention: different enzyme and other by-products are generated by microbial fermentation specified raw material, promotes hair
Ferment efficiency, shortens fermentation period；The middle effective component of Chinese medicine and fruits and vegetables retains its activity by microbial fermentation can completely,
Improve the health care effectiveness of ferment；It is fermented using oil expression by-product grouts as culture medium and extracts Tea Saponin and phytic acid promotion system
Zymogenic growth and efficiency, improve resource utilization.
Specific embodiment
Below with reference to embodiment, the present invention is further described.
Embodiment 1
(1) by ratio of weight and the number of copies, 15 parts of grape fruit are taken, peeling separates fruit and pericarp, then fruit squeezed the juice, gained fruit
Retain pulp in juice, while pericarp be dried to that water content is 10%, and be ground into 15 mesh powders, obtain grape grapefruit juice and
Grapefruit peel powder；10 parts of malt are taken, takes out and smashs to pieces after adding water enchroachment (invasion) to steep 20h, saccharomycete is added and ferments, obtains malt fermentation
Then malt fermentation liquid is uniformly mixed by liquid with grape grapefruit juice, the swelling that granularity is 100 mesh is added by the 6% of total liquid quality
Soil, with vinegar acid for adjusting pH to 1 ~ 2, at room temperature after stirring and leaching 1h, centrifugation removal bentonite precipitating obtains extracting solution；
The water temperature that the malt is soaked in water is 15 ~ 20 DEG C, and specific fermentation process is as follows: its quality is added in the malt after smashing to pieces
1 times of water and 1% sodium chloride, be inoculated with 3% saccharomycete and ferment at 35 DEG C 6h, obtain malt fermentation liquid.
(2) by ratio of weight and the number of copies, 15 parts of scutellariae,radix are taken, 8 times of distilled water measured are added after being crushed to 40 mesh, decocts 1.5h and obtains
To radix scutellariae medical fluid, after radix scutellariae medical fluid is cooled to 40 DEG C, it is centrifuged 12 ~ 15 minutes under 2000r/min, takes supernatant to get arriving
Radix scutellariae extracting solution；1.0% peptone is added by the mass ratio of radix scutellariae extracting solution, 0.05% sodium chloride, adjusting pH is 7.0 ~ 8.6, high
Pressure sterilize simultaneously be cooled to room temperature to obtain radix scutellariae fermentation liquid I, then in radix scutellariae fermentation liquid I be inoculated with compound bacteria I, 45 DEG C, 100 ~
200r/min shaking table top fermentation 4d, obtains radix scutellariae fermentation liquid II；
By I volume basis of radix scutellariae fermentation liquid, the compound bacteria I is the Bacillus natto for 4% kind of age being 12hBacillus nattoBacterium solution
It is 14h Lactobacillus rhamnosus with 3% kind of ageLactobacillus rhamnosusBacterium solution combination；
(3) by ratio of weight and the number of copies, 10 parts of dregs of rapeseed cake, 10 parts of tea-cake dregs are taken, is uniformly mixed after being crushed to 50 mesh, obtains grouts
Powder；Grapefruit peel powder, grouts powder are uniformly mixed, the water of 10 times of amounts is added, high pressure sterilization, obtains fermentation liquid after mixing
III, after its cooling, inoculation compound bacteria II is fermented at 35 DEG C；
By III volume basis of fermentation liquid, the compound bacteria II is the bacillus subtilis for 4% kind of age being 12hBacillus subtilisThe aspergillus niger that bacterium solution and 2 ~ 4% kinds of ages are 12hAspergillus nigerBacterium solution combination；
(4) after fermentation liquid III ferments 6 days, extracting solution and radix scutellariae fermentation liquid II is added, while it is de- that III volume ratio 5% of fermentation liquid is added
The chitosan solution that acetyl degree is 50% is uniformly mixed after continuing fermentation 7 days, by filtering fermentation liquor, obtains clarified solution, will clarify
The ferment of coordinating intestines and stomach can be obtained in liquid high pressure sterilization 30min at 70 DEG C after cooling.
Embodiment 2
(1) by ratio of weight and the number of copies, 20 parts of grape fruit are taken, peeling separates fruit and pericarp, then fruit squeezed the juice, gained fruit
Retain pulp in juice, while pericarp be dried to that water content is 20%, and be ground into 20 mesh powders, obtain grape grapefruit juice and
Grapefruit peel powder；20 parts of malt are taken, adds water enchroachment (invasion) bubble to take out afterwards for 24 hours and smashs to pieces, saccharomycete is added and ferments, obtain malt fermentation
Then malt fermentation liquid is uniformly mixed by liquid with grape grapefruit juice, the swelling that granularity is 120 mesh is added by the 8% of total liquid quality
Soil, with vinegar acid for adjusting pH to 1 ~ 2, at room temperature after stirring and leaching 3h, centrifugation removal bentonite precipitating obtains extracting solution；
The water temperature that the malt is soaked in water is 20 DEG C, and specific fermentation process is as follows: its quality 1.5 is added in the malt after smashing to pieces
Times water and 1.2% sodium chloride, be inoculated with 3% saccharomycete and ferment at 35 DEG C 6h, obtain malt fermentation liquid.
(2) by ratio of weight and the number of copies, 20 parts of scutellariae,radix are taken, 10 times of distilled water measured are added after being crushed to 50 mesh, decocts 2h and obtains
It to radix scutellariae medical fluid, after radix scutellariae medical fluid is cooled to 60 DEG C, is centrifuged 15 minutes under 2000r/min, takes supernatant to get radix scutellariae is arrived
Extracting solution；1.0% peptone is added by the mass ratio of radix scutellariae extracting solution, 0.05% sodium chloride, adjusting pH is 8.6, and high pressure sterilization is simultaneously
It is cooled to room temperature to obtain radix scutellariae fermentation liquid I, compound bacteria I is then inoculated in radix scutellariae fermentation liquid I, in 55 DEG C, 100 ~ 200r/min
Shaking table 4 ~ 6d of top fermentation, obtains radix scutellariae fermentation liquid II；
By I volume basis of radix scutellariae fermentation liquid, the compound bacteria I is the Bacillus natto for 6% kind of age being 14hBacillus nattoBacterium solution
It is 16h Lactobacillus rhamnosus with 5% kind of ageLactobacillus rhamnosusBacterium solution combination；
(3) by ratio of weight and the number of copies, 20 parts of dregs of rapeseed cake, 20 parts of tea-cake dregs are taken, is uniformly mixed after being crushed to 100 mesh, obtains grouts
Powder；Grapefruit peel powder, grouts powder are uniformly mixed, the water of 20 times of amounts is added, high pressure sterilization, obtains fermentation liquid after mixing
III, after its cooling, inoculation compound bacteria II is fermented at 40 DEG C；
By III volume basis of fermentation liquid, the compound bacteria II is the bacillus subtilis for 6% kind of age being 14hBacillus subtilisThe aspergillus niger that bacterium solution and 4% kind of age are 14hAspergillus nigerBacterium solution combination；
(4) after fermentation liquid III ferments 7 days, extracting solution and radix scutellariae fermentation liquid II is added, while it is de- that III volume ratio 10% of fermentation liquid is added
The chitosan solution that acetyl degree is 60% is uniformly mixed after continuing fermentation 7 days, by filtering fermentation liquor, obtains clarified solution, will clarify
The ferment of coordinating intestines and stomach can be obtained in liquid high pressure sterilization 50min at 80 DEG C after cooling.
Embodiment 3
(1) by ratio of weight and the number of copies, 18 parts of grape fruit are taken, peeling separates fruit and pericarp, then fruit squeezed the juice, gained fruit
Retain pulp in juice, while pericarp be dried to that water content is 15%, and be ground into 20 mesh powders, obtain grape grapefruit juice and
Grapefruit peel powder；15 parts of malt are taken, takes out and smashs to pieces after adding water enchroachment (invasion) to steep 20h, saccharomycete is added and ferments, obtains malt fermentation
Then malt fermentation liquid is uniformly mixed by liquid with grape grapefruit juice, the swelling that granularity is 110 mesh is added by the 7% of total liquid quality
Soil, with vinegar acid for adjusting pH to 1 ~ 2, at room temperature after stirring and leaching 2h, centrifugation removal bentonite precipitating obtains extracting solution；
The water temperature that the malt is soaked in water is 15 DEG C, and specific fermentation process is as follows: its quality 1.2 is added in the malt after smashing to pieces
Times water and 1.1% sodium chloride, be inoculated with 3% saccharomycete and ferment at 35 DEG C 6h, obtain malt fermentation liquid.
(2) by ratio of weight and the number of copies, 18 parts of scutellariae,radix are taken, 9 times of distilled water measured are added after being crushed to 15 mesh, decocts 2h and obtains
Radix scutellariae medical fluid is centrifuged 13 minutes under 2000r/min after radix scutellariae medical fluid is cooled to 50 DEG C, supernatant is taken to mention to get to radix scutellariae
Take liquid；1.0% peptone is added by the mass ratio of radix scutellariae extracting solution, 0.05% sodium chloride, adjusting pH is 7.6, and high pressure sterilization is simultaneously cold
But radix scutellariae fermentation liquid I is obtained to room temperature, compound bacteria I is then inoculated in radix scutellariae fermentation liquid I, is shaken in 50 DEG C, 100 ~ 200r/min
Bed top fermentation 5d, obtains radix scutellariae fermentation liquid II；
By I volume basis of radix scutellariae fermentation liquid, the compound bacteria I is the Bacillus natto for 5% kind of age being 13hBacillus nattoBacterium solution
It is 15h Lactobacillus rhamnosus with 4% kind of ageLactobacillus rhamnosusBacterium solution combination；
(3) by ratio of weight and the number of copies, 15 parts of dregs of rapeseed cake, 16 parts of tea-cake dregs are taken, is uniformly mixed after being crushed to 80 mesh, obtains grouts
Powder；Grapefruit peel powder, grouts powder are uniformly mixed, the water of 15 times of amounts is added, high pressure sterilization, obtains fermentation liquid after mixing
III, after its cooling, inoculation compound bacteria II is fermented at 37 DEG C；
By III volume basis of fermentation liquid, the compound bacteria II is the bacillus subtilis for 5% kind of age being 13hBacillus subtilisThe aspergillus niger that bacterium solution and 3% kind of age are 13hAspergillus nigerBacterium solution combination；
(4) after fermentation liquid III ferments 6 days, extracting solution and radix scutellariae fermentation liquid II is added, while it is de- that III volume ratio 7% of fermentation liquid is added
The chitosan solution that acetyl degree is 55% is uniformly mixed after continuing fermentation 7 days, by filtering fermentation liquor, obtains clarified solution, will clarify
The ferment of coordinating intestines and stomach can be obtained in liquid high pressure sterilization 40min at 75 DEG C after cooling.
For guarantee ferment safety prepared by the present invention, it is evaluated through the following experiment:
1, simulate the gastric juice and the resistance test of intestinal juice
Take the hydrochloric acid 16.4mL of 100g/L that distilled water is added to dilute, making pH value is respectively 1.5,2.5 and 3.5, takes 100mL dilute hydrochloric acid molten
Liquid is separately added into 1g pepsin, dissolves it sufficiently, obtains simulate the gastric juice, and miillpore filter degerming (0.22 μm) is spare.Take phosphoric acid
Potassium dihydrogen 6.8g adds water 500mL to make to dissolve, and adjusts pH value to 6.8 with 0.1moL/L sodium hydroxide solution；Separately take trypsase
10g adds water 100mL to make to dissolve, and after two liquid are mixed, is diluted with water to 1000ml, obtains simulated intestinal fluid, miillpore filter degerming
(0.22 μm) is spare.
Taking the ferment solution of the preparation of 1mL embodiment 1 ~ 3 to be added in the simulate the gastric juice of 9mL respectively, (i.e. ten times dilute step by step
Release), and mix well on the oscillator rapidly, it is subsequently placed in 30 ~ 45 DEG C of 2 ~ 4h of stationary culture.Respectively in 1h, 2h, 3h, 4h
When take out culture solution and count remaining viable count immediately, be compared with former viable count, the results showed that, Viable detection is distinguished
For 98.6% and 98.9%.Then each 1mL of culture solution for digesting different time in simulated gastric fluid is taken, is inoculated in 9mL pH respectively
Value to be placed in 30 ~ 45 DEG C of 2 ~ 4h of stationary culture in 6.8 simulated intestinal fluid, and respectively 0,3,6, sample for 24 hours, measure its viable bacteria
Number, is compared, the results showed that Viable detection is 99% with former viable count.
2, the ferment that embodiment 1 ~ 3 is obtained according to the bowel relaxing functions detection method of " health food inspection and assessment technique specification "
Element is evaluated, specific as follows:
Test method:
1.1 experimental animals select adult female mice, weight 18~22g, and every group 10；Method is that modeling medicine is given in oral stomach-filling
Object compound diphenoxylate, establishes Constipated mice, and stomach-filling prepared Chinese ink measures the first grain row melena defecation time of mouse, in 5 hours
Defecation grain number and defecation weight, the defecation situation of Lai Fanying model mice.
1.2 grouping situations are as follows:
Group 1: the ferment of stomach-filling embodiment 1；
Group 2: the ferment of stomach-filling embodiment 2；
Group 3: the ferment of stomach-filling embodiment 3；
Above-mentioned group mouse is taken twice daily, and each 10ml takes after being diluted with warm water；
Group 4: model control group, the physiological saline of stomach-filling equivalent；
Group 5: blank control group, the physiological saline of stomach-filling equivalent.
1.3 model foundation
After given the test agent 7 days, each group mouse is deprived of food but not water 16 hours.
5 blank control groups of group organize 1 ~ 4 stomach-filling and give compound diphenoxylate to distilled water.
The specific method of 1.4 index determinings
For group 1 ~ 4 to after compound diphenoxylate 0.5 hour, group 1 ~ 5 gives mouse prepared Chinese ink stomach-filling, and the equal single cage of animal is raised, normally
Since drinking-water feed record every small mouse's head grain row's melena time, arrange melena grain number and weight in 5 hours filling prepared Chinese ink.Specifically
As a result such as table 1.
1 each group mouse defecation result of table
| Group | First grain arranges melena time (h) | Melena grain number is arranged in 5 hours | Melena weight (g) is arranged in 5 hours |
| 1 | 0.80 | 18 | 2.50±0.51 |
| 2 | 0.83 | 16 | 2.20±0.53 |
| 3 | 0.78 | 19 | 2.54±0.43 |
| 4 | 4.20 | 5 | 0.88±0.25 |
| 5 | 1.05 | 14 | 2.11±0.44 |
From the above results, more significant by the resulting ferment relax bowel and defecation effect of the embodiment of the present invention 1 ~ 3, even better than group
5 blank control groups.
Although an embodiment of the invention has been described, it will be understood by those skilled in the art that: do not departing from this
These embodiments can be carried out with a variety of change, modification, replacement and modification in the case where the principle and objective of invention, it is of the invention
Range is defined by the claims and their equivalents.
Claims (7)
1. a kind of preparation method of coordinating intestines and stomach function ferment, which comprises the following steps:
(1) by ratio of weight and the number of copies, 15 ~ 20 parts of grape fruit are taken, peeling separates fruit and pericarp, then fruit squeezed the juice, institute
It obtains and retains pulp in fruit juice, while pericarp solarization dry doubling is crushed into powder end, obtain grape grapefruit juice and grapefruit peel powder；Take wheat
10 ~ 20 parts of bud, add water enchroachment (invasion) bubble 20 ~ for 24 hours afterwards take out smash to pieces, be added saccharomycete ferment, malt fermentation liquid is obtained, then by wheat
Bud fermentation liquid is uniformly mixed with grape grapefruit juice, bentonite is added by the 6 ~ 8% of total liquid quality, with vinegar acid for adjusting pH to 1 ~ 2, room
After the lower 1 ~ 3h of stirring and leaching of temperature, centrifugation removal bentonite precipitating obtains extracting solution；
(2) by ratio of weight and the number of copies, 15 ~ 20 parts of scutellariae,radix are taken, the distillations that granularity is 8 ~ 10 times of amounts of addition after 40 ~ 50 mesh are crushed to
Water decocts 1.5 ~ 2h and obtains radix scutellariae medical fluid, and after radix scutellariae medical fluid is cooled to 40 ~ 60 DEG C, 12 ~ 15 points are centrifuged under 2000r/min
Clock takes supernatant to get radix scutellariae extracting solution is arrived；By radix scutellariae extracting solution mass ratio be added 1.0% peptone, 0.05% sodium chloride,
Adjusting pH is 7.0 ~ 8.6, and high pressure sterilization simultaneously is cooled to room temperature to obtain radix scutellariae fermentation liquid I, is then inoculated in radix scutellariae fermentation liquid I multiple
Combined bacteria I obtains radix scutellariae fermentation liquid II in 45 ~ 55 DEG C, 100 ~ 200r/min shaking table 4 ~ 6d of top fermentation；
By I volume basis of radix scutellariae fermentation liquid, the compound bacteria I be 4 ~ 6% Bacillus nattoBacillus nattoBacterium solution and 3 ~ 5% mouse
Lee's sugar lactobacillusLactobacillus rhamnosusBacterium solution combination；
(3) by ratio of weight and the number of copies, 10 ~ 20 parts of dregs of rapeseed cake, 10 ~ 20 parts of tea-cake dregs are taken, is uniformly mixed after being crushed to 50 ~ 100 mesh,
Obtain grouts powder；Grapefruit peel powder, grouts powder are uniformly mixed, the water of 10 ~ 20 times of amounts is added, high pressure is gone out after mixing
Bacterium obtains fermentation liquid III, and after its cooling, inoculation compound bacteria II is fermented at 35 ~ 40 DEG C；
By III volume basis of fermentation liquid, the compound bacteria II be 4 ~ 6% bacillus subtilisBacillus subtilisBacterium solution
With 2 ~ 4% aspergillus nigerAspergillus nigerBacterium solution combination；
(4) after fermentation liquid III ferments 6 ~ 7 days, extracting solution and radix scutellariae fermentation liquid II is added, at the same be added III volume ratio 5 of fermentation liquid ~
10% chitosan solution is uniformly mixed after continuing fermentation 6 ~ 7 days, by filtering fermentation liquor, obtains clarified solution, by clarified solution in 70 ~
The ferment of coordinating intestines and stomach can be obtained in 30 ~ 50min of high pressure sterilization at 80 DEG C after cooling.
2. a kind of preparation method of coordinating intestines and stomach function ferment according to claim 1, which is characterized in that step (1) institute
It states grape fruit pericarp and is dried to that water content is 10 ~ 20%, be crushed to 15 ~ 20 mesh.
3. a kind of preparation method of coordinating intestines and stomach function ferment according to claim 1, which is characterized in that step (1) institute
Stating the water temperature that malt is soaked in water is 15 ~ 20 DEG C, and specific fermentation process is as follows: its quality 1 ~ 1.5 is added in the malt after smashing to pieces
Times water and 1 ~ 1.2% sodium chloride, be inoculated with 3% saccharomycete and ferment at 35 DEG C 6h, obtain malt fermentation liquid.
4. a kind of preparation method of coordinating intestines and stomach function ferment according to claim 1, which is characterized in that step (1) institute
The swelling soil granularity stated is 100 ~ 120 mesh.
5. a kind of preparation method of coordinating intestines and stomach function ferment according to claim 1, which is characterized in that step (2) institute
Stating compound bacteria I is the Bacillus natto for being 12 ~ 14h by kind of ageBacillus nattoIt is 14 ~ 16h Lactobacillus rhamnosus with kind of ageLactobacillus rhamnosusCombination.
6. a kind of preparation method of coordinating intestines and stomach function ferment according to claim 1, which is characterized in that step (3) institute
Stating compound bacteria II is to plant the bacillus subtilis that age is 12 ~ 14hBacillus subtilisThe aspergillus niger of sumAspergillus nigerCombination.
7. a kind of preparation method of coordinating intestines and stomach function ferment according to claim 1, which is characterized in that step (4) institute
The deacetylating degree of chitosan stated is 50 ~ 60%.
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| CN105146614A (en) * | 2015-08-01 | 2015-12-16 | 周学义 | Functional Chinese dwarf cherry seed ferment, ferment beverage and production method of ferment beverage |
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| EP3235386A1 (en) * | 2016-04-21 | 2017-10-25 | AMC Innova Juice & Drinks, S.L. | Process for obtaining juice from fruit peel |
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| WO2010061383A1 (en) * | 2008-11-26 | 2010-06-03 | Biodalia Microbiological Technologies Ltd. | A method of in-situ enrichment of foods with fructan |
| CN106107386A (en) * | 2016-07-26 | 2016-11-16 | 厦门益力康生物科技有限公司 | A kind of multi-functional ferment purifying the intestines and stomach and preparation method thereof |
| CN106174519A (en) * | 2016-07-26 | 2016-12-07 | 厦门益力康生物科技有限公司 | A kind of activity composite enzyme and preparation method thereof |
| CN106262824A (en) * | 2016-08-12 | 2017-01-04 | 青岛康大万叶生物科技有限公司 | A kind of production method of Chinese herbal medicine ferment stock solution |
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| CN105146614A (en) * | 2015-08-01 | 2015-12-16 | 周学义 | Functional Chinese dwarf cherry seed ferment, ferment beverage and production method of ferment beverage |
| CN105167072A (en) * | 2015-08-02 | 2015-12-23 | 周学义 | Production method of functional Chinese wolfberry fruit enzyme and product thereof |
| EP3235386A1 (en) * | 2016-04-21 | 2017-10-25 | AMC Innova Juice & Drinks, S.L. | Process for obtaining juice from fruit peel |
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