CN109813688A - Method for evaluating quality of fulvic acid solution and method for producing fulvic acid solution - Google Patents
Method for evaluating quality of fulvic acid solution and method for producing fulvic acid solution Download PDFInfo
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- CN109813688A CN109813688A CN201811253300.5A CN201811253300A CN109813688A CN 109813688 A CN109813688 A CN 109813688A CN 201811253300 A CN201811253300 A CN 201811253300A CN 109813688 A CN109813688 A CN 109813688A
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Abstract
The present invention relates to a method for evaluating the quality of a fulvic acid solution and a method for producing a fulvic acid solution. Provided are an evaluation method for obtaining a large amount of fulvic acid inexpensively and easily, and a method for producing a fulvic acid solution. A method for evaluating the quality of a fulvic acid solution and a method for producing a fulvic acid solution based on the above evaluation method, wherein a cultured mushroom bed, the main component of which is wood chips, is used, the quality of the mushroom bed is judged based on the appearance and brittleness of the bed, and the quality of a solution derived from the mushroom bed is judged based on pH and color.
Description
Technical field
The present invention relates to the manufacturing methods of the evaluation method of fulvic acid solution superiority and inferiority and fulvic acid solution.
Background technique
Fulvic acid (fulvic acid, fulvic acid) refer to Plant death and rot after humus group general name.State
Border humus association (Internat ional Humic Substances Society, hereinafter referred to as IHSS) is to fulvic acid
It is defined.
Application for fulvic acid, using the application (patent document that the purpose of harvest yield when increasing rice cropping is representative
1), there are also by seafood raise for the purpose of application；To improve health, beauty as nutrition fortifier ingredient, cosmetic composition
Application for the purpose of appearance etc..
However, the main component of humus is humic acid, and compared with humic acid, the research of the fulvic acid as micro constitutent
Status be not yet to be developed.In particular, there are time-consuming when carrying out according to method shown in IHSS in terms of manufacturing fulvic acid
The high problem with cost.
In this regard, it is disclosed in document: by immature bark compost, the sawdust, rice for not aggravating rotten plant
The organic materials such as grass, rice husk, dewatered cake impregnate the manufacturing method (patent document 2) to manufacture in an acidic solution；In high temperature
In the air of high pressure, manufacturing method (the patent text that makes humus be degraded by rudimentary oxidation of coal and be extracted using high-temperature-hot-water
It offers 3)；The manufacturing method (patent document 4) of curing acquisition is carried out by the peat and water that acquire from underground.
However, the invention of patent document 2 is in terms of needing the strongly acidic solutions such as sulfuric acid solution, hydrochloric acid, acetic acid, there are expenses
The problem of, processing when need with caution pay attention to the problem of.The invention of patent document 3 is also same.The invention of patent document 4 is utilized
Peat and water, but need heat drying in vinyl house, by fulvic acid aqueous solution curing step as unit of year.
As the cheap raw material for manufacturing fulvic acid, disclose using mushroom give up bacterium bed (mushroom culture after bacterium
Bed) a possibility that (non-patent literature 1).However, the method according to shown in IHSS or in this way on the basis of method when exist
With same problems mentioned above.
Existing technical literature
Patent document
Patent document 1: Japanese Unexamined Patent Publication 2006-151706 bulletin
Patent document 2: Japanese Unexamined Patent Publication 2010-148398 bulletin
Patent document 3: Japanese Unexamined Patent Publication 2004-269484 bulletin
Patent document 4: Japanese Unexamined Patent Publication 2014-162723 bulletin
Non-patent literature
Non-patent literature 1: " 28 year of Heisei CO2Discharge cuts down countermeasure and strengthens induction type Ji Shu Kai Development Actual Certificate cause Abolished
The place of production disappears モ デ Le real Certificate bacterium bed バ イ オ マ ス turning sludge into fuel skill art with opening development To I Ru Abolished waste resource お I び
Achievement Report book " (28 year of Heisei CO2Mitigation options reinforcement technique exploitation/demonstrative project utilizes discarded bacterium bed biomass fuel
Change technological development and carry out the recycling of waste and the demonstration result report of the local consumption model of local production) (Heisei 29
March in year) (u'eno village mushroom center Co., Ltd., star university, Japanese industry university)
Summary of the invention
Problems to be solved by the invention
The problem to be solved by the present invention is that provide: without expensive raw material, drug, complexity operation and even if
There is no special knowledge and can easily obtain the evaluation method of a large amount of fulvic acid and fulvic acid based on aforementioned evaluation method molten
The manufacturing method of liquid.
The solution to the problem
The first invention completed to solve aforementioned problems is the evaluation method of fulvic acid solution superiority and inferiority, and feature exists
In when extracting fulvic acid solution from the mushroom bacterium bed after culture, the main component of aforementioned bacterium bed is sawdust, the evaluation side
Method includes the following steps: pH verification step: after aforementioned mushroom bacterium bed is enclosed in container, to the molten of dissolution to aforesaid receptacle bottom
The pH of liquid is confirmed；Color verification step: the color of previous solu is confirmed；And judgment step: based in aforementioned pH
The pH confirmed in verification step and the color confirmed in aforementioned color verification step, in pH be 4.0 or less and color is in Meng
In the Munsell hue ring (40 form and aspect) of Sai Er color system (Munsell color system), with it is water-soluble go out when form and aspect be
2.5YR~10YR, with the mixed liquor of wood vinegar or bamboo vinegar or wood vinegar and bamboo vinegar dissolve out when form and aspect be 10YR~10Y
In the case where, the solution is judged as excellent.In addition, the second invention is a kind of manufacturing method of fulvic acid solution, feature exists
In in the manufacturing method by the mushroom bacterium bed manufacture fulvic acid solution after cultivating, the main component of aforementioned bacterium bed is sawdust, institute
It states manufacturing method and includes the following steps: that step 1 is rapid: will visually confirm the height in aforementioned bacterium bed, width, length direction
Being covered with mycelia and sensory evaluation on the whole, to be that the high mushroom bacterium bed of brittleness is evaluated as excellent, is evaluated as in addition to this bad；Step 2
It is rapid: aforementioned mushroom bacterium bed being enclosed in container, makes solution dissolution to aforesaid receptacle bottom；Third step: in aforementioned step 1
It is be evaluated as excellent mushroom bacterium bed in rapid, the pH of the dissolution fluid of aforementioned second step is confirmed；And step 4 is rapid: for
The pH confirmed in aforementioned third step is the color of 4.0 solution below and is evaluated as bad mushroom bacterium bed in aforementioned 1 step
The color of the solution of aforementioned second step, by the color of any one in the Munsell hue ring (40 form and aspect) of Munsell colour system
It is mutually set as 0, rotates clockwise+20 relative to aforementioned form and aspect, rotation -20 is counterclockwise come when indicating, the form and aspect of another one are in+
Solution in 4~+6 or -4~-6 hue range is judged as excellent, and excellent solution will be judged as in aforementioned step 4 is rapid and is made
For fulvic acid solution.In addition, the 3rd invention is a kind of manufacturing method of fulvic acid solution, which is characterized in that by the mushroom after cultivating
Mushroom bed manufactures in the manufacturing method of fulvic acid solution, and the main component of aforementioned bacterium bed is sawdust, and the manufacturing method includes such as
Lower step: step 1 is rapid: will visually confirm the height in aforementioned bacterium bed, width, length direction and be covered with mycelia on the whole
And to be that the high mushroom bacterium bed of brittleness is evaluated as excellent for sensory evaluation, is evaluated as in addition to this bad；Second step: by aforementioned mushroom bacterium bed
It is enclosed in container together with the mixed liquor of bamboo vinegar with wood vinegar or bamboo vinegar or wood vinegar, makes solution dissolution to aforementioned appearance
Device bottom；Third step: for being be evaluated as excellent mushroom bacterium bed in aforementioned step 1 is rapid, to the dissolution fluid of aforementioned second step
PH confirmed；And step 4 is rapid: being lower than aforementioned wood vinegar or aforementioned bamboo vinegar for the pH confirmed in aforementioned third step
The color of the solution of the pH of the mixed liquor of liquid or aforementioned wood vinegar and bamboo vinegar and aforementioned wood vinegar or aforementioned bamboo vinegar or
The color of the mixed liquor of the aforementioned wood vinegar of person and bamboo vinegar, will in the Munsell hue ring (40 form and aspect) of Munsell colour system
The form and aspect of any one are set as 0, rotate clockwise+20 relative to aforementioned form and aspect, rotation -20 is counterclockwise come when indicating, by another one
Form and aspect be in+4~+6 or -4~-6 hue range in solution be judged as excellent, will be judged as in aforementioned step 4 is rapid
Excellent solution is as fulvic acid solution.Color refers to the constituent element of color in the present invention, for example, can enumerate referred to as form and aspect,
Three attributes of chroma, lightness.Color, which can be in the constituent element of color, in the present invention judges useful one to aforementioned superiority and inferiority
A factor can also be comprehensive sexual factor.
The effect of invention
The present invention is based on pH and color to comment the superiority and inferiority of the mushroom bacterium bed solution after the culture for becoming fulvic acid raw material
Valence, therefore can expect inexpensively and easily to obtain a large amount of fulvic acid.In particular, being Composting for the acidification for bacterium bed of giving up
Main restraining factors this point have not focused on all the time, and by it is aforementioned evaluation have found acidification aggravation useless bacterium bed
Serviceability, it is possible thereby to expect inexpensively to supply fulvic acid with the purpose of breed of crop, raising purpose of seafood etc..
Detailed description of the invention
Fig. 1 is derived from mushroom and gives up that (horizontal axis is wavelength of fluorescence (nm), the longitudinal axis is excitation wave for the 3D fluorescence spectrum of solution of bacterium bed
It is long (nm), following same).
Fig. 2 is the 3D fluorescence spectrum of fulvic acid titer.
Fig. 3 is derived from the 3D fluorescence spectrum of the solution of the bacterium bed in mushroom cultivation.
Fig. 4 is to be given up the 3D fluorescence spectrum of the liquid obtained after bacterium bed is handled using autoclave to mushroom.
Specific embodiment
[embodiment 1]
Embodiments of the present invention will be described below.
(1) bacterium bed
As sawdust, use by making hardwood sawdust as main component, the wheat bran as other auxiliary elements, corn
The mushroom bacterium bed of the compositions such as skin and root, fossil shell.Construct is not limited for the sawdust of bacterium bed, such as can be only by sawdust structure
At.In addition, the cultivation number for the mushroom in same bacterium bed does not also limit, but cultivate in the richness in the more solution of number
The bigger tendency of acid concentration is desired.It should be noted that useless bacterium bed, bacterium, fulvic acid as described below in the present invention
Analysis method is identical as the content of non-patent literature 1.
(2) compliance test result for bacterium bed of giving up
(formula is ground in the north of non-patent literature 1 to the useless bacterium bed of mushroom is originated from filter (0.45 microfilter of ADVANTEC)
Bacterium bed) and from mushroom cultivate in bacterium bed solution in include ingredient be filtered after for 3D spectrofluorimetry (point
Analysis is the result is that table 1: main dissolved element) (device: Hitachi, Ltd F-4500 type spectrofluorophotometer).Solution be with
Time passes through the substance oozed out naturally from bacterium bed.
[table 1]
| Useless bacterium bed | Bacterium bed in cultivation | |
| Dissolved element | Fulvic acid | Humic acid |
Analysis the result is that: what useless bacterium bed solution was observed near excitation wavelength 340nm, near wavelength of fluorescence 400nm
Peak (Fig. 1: horizontal axis is wavelength of fluorescence, the longitudinal axis is excitation wavelength, following same).The result is suggested that due to obviously observing in richness
The peak (Fig. 2) of acid, to show there is fulvic acid in the solution.Solution from the bacterium bed in cultivation is in wavelength of fluorescence 530nm
Nearby, excitation wavelength 430nm nearby observes that there is larger offset at peak, fluorescent places and aforementioned useless bacterium bed solution, suggests: with richness
In acid compare, a possibility that amount of the humic acid as the rotten main component for planting ingredient is more (Fig. 3).According to result above
It suggests that: fulvic acid being extracted by water about bacterium bed is originated from, compared with being originated from the bacterium bed in cultivating, being originated from useless bacterium bed can be with
It obtains more fulvic acid and impurity is few (raw material as manufacture fulvic acid is more high-quality).In addition, being used in the embodiment
Mushroom bacterium bed, but same effect is also suggested that other mushroom bacterium beds.
(3) the superiority and inferiority judgement for bacterium bed of giving up
Bacterium bed be repeated using various bacterium beds contain fulvic acid in moisture and analyze, as a result known to: it is pure in order to obtain
Du Genggao, a large amount of fulvic acid good bacterium bed can be by visual observation by appearance, the i.e. distribution of the mycelia on bacterium bed surface
To be judged.Specifically, being able to observe that the case where white to milky mycelia is integrally distributed in bacterium bed surface
The dissolution of fulvic acid can be confirmed.
Herein, integrally distribution does not imply that mycelia must cover the state of bacterium bed whole surface, as long as but being capable of meat
Eye confirms the height in bacterium bed, width, length direction and has substantially been covered with mycelia on the whole.In addition, the color root of mycelia
It is different and different according to the type of mushroom, therefore white to milky situation is only an example.In addition, for for this analysis
Useless bacterium bed, it is known that compared with the color of non-prime bacterium bed, the color of good bacterium bed itself seems deeper, can also pass through
Color is judged.It can thus be appreciated that the appearance information of useless bacterium bed judges that element is useful as superiority and inferiority.
In addition, it will be appreciated that: the appearance confirmation of mycelia is not only carried out, is confirmed and energy together with the brittleness with bacterium bed
Enough judge whether it is the good bacterium bed for obtaining fulvic acid.In the present invention, brittleness refers to the degree that bacterium bed is easily destroyed.It is right
Bacterium bed before planting bacterium or in mycelia cultivation, crisp degree are as follows: by holding bacterium bed top with one hand and lifting journey upwards
The power of degree, the part held are thereafter easily released.On the other hand, the bacterium bed such as whole surface that can obtain a large amount of fulvic acid is applied
It is hardened as covering, that is, uses and hold bacterium bed with the power equal extent or stronger power for being applied to aforementioned bacterium bed and will not be easy
Ground removing.It is difficult to define the brittleness of bacterium bed rigorously.However, passing through the analysis repeated about the present invention, without tight
When simply being evaluated under the premise of the inspection of lattice, bacterium bed surface is directly contacted, passes through following aesthetic evaluation:
" hard (bacterium bed surface is the state being hardened, and is placed on desk even if being pressed with finger with bacterium bed to the power of transverse shifting degree
Bacterium bed side, finger will not be fallen into bacterium bed) ", " slightly soft (bacterium bed surface is slightly soft state, is used in the same manner as aforementioned
When finger presses, finger is easily trapped into inside bacterium bed) ", " which is not (not soft not hard) ".Thus it suggests that: low in brittleness
In the case where the bacterium bed of (crisp), it is mixed with what humic acid, sawdust etc. were dissolved out from bacterium bed construct in obtained fulvic acid solution
Impurity；In the case where the bacterium bed of brittleness height (survivable), obtained fulvic acid solution has different colors in the height direction
Color region.Herein, as long as referring to that for example solution form and aspect have different parts (at solution bottom with different color areas
The case where portion's retained sediments object, is more, might not entire solution be same form and aspect).That is, suggesting that the high bacterium bed of brittleness can be with
Obtain the few fulvic acid solution of impurity.
By these opinions, can consider as shown in the table (table 2) for obtaining the superiority and inferiority judgement of bacterium bed of fulvic acid.
For being judged as excellent bacterium bed, have the tendency that humic acid is not easy to dissolve out, fulvic acid is easy dissolution.Herein, the bacterium bed in the present invention
Superiority and inferiority judgement should not strictly be construed to the sentence of following table, can also be replaced with substantially equivalent other indexs etc..
[table 2]
Zero: excellent
(4) the superiority and inferiority judgement of fulvic acid solution
As the method for collecting fulvic acid solution, there are as below methods: bacterium bed being enclosed in container, is collected as time goes by
The method that the moisture of bacterium bed flows out to the substance of container bottom；With by bacterium bed dipping in water in the state of enclose container in, etc.
The method being acquired again is flowed out to fulvic acid.It is not limited herein for container, metal container, glass system can be used
Container, resin container also do not limit capacity.For whether carrying out closed, the gasification of solution in order to prevent to container
And it is desired for air-tight state, but be not meant to be stringent air-tight state.Solution takes environment also not have under room temperature, normal pressure
Problem, but to avoiding the breeding of microorganism and being desired for 15 DEG C or less.Herein, in the useless bacterium bed about 20g of fine crushing
Be added purified water 300g, is carried out under 121 DEG C, 2 atmospheric pressure using autoclave 20 minutes handle, as a result obtain with it is aforementioned richness in
Different (black) liquid of the form and aspect of acid solution.It confirmed to dissolve out together with fulvic acid based on 3D pattern analysis results (Fig. 4)
A large amount of impurity suggest that forcing to extract fulvic acid under high temperature environment is not appropriate for obtaining the fulvic acid of purity is high.
3D spectrofluorimetry is carried out to dipping water when being originated from the solution of bacterium bed, impregnating bacterium bed in water, it is thus identified that
PH and fulvic acid whether there is relationship.The result confirmed: the amount of (pH is lower) fulvic acid increases when pH is 4.0 or less；PH is
The amount of fulvic acid is few when 5.0 or more, amount of humic acid increases.The solution obtained by being judged as excellent bacterium bed in aforementioned (3)
Have as time goes by pH be reduced to 4.0 tendencies below.
Similarly, it is thus identified that solution from bacterium bed, by bacterium bed dipping in water when dipping water color and fulvic acid
With the presence or absence of relationship.In the Munsell hue ring (40 form and aspect) of Munsell colour system, for being originated from aforementioned third step
It is judged as the solution of excellent mushroom bacterium bed and is originated to be judged as in the solution of bad mushroom bacterium bed, any form and aspect are set as
0, with rotate clockwise+20, rotation -20 is counterclockwise come when indicating, be in+4 with the form and aspect that the form and aspect 0 are benchmark another one~+
When in 6 or -4~-6 hue range, it can be confirmed that the amount of fulvic acid in the solution for being originated from and being judged as excellent mushroom bacterium bed increases
It is more, not within the above range the case where humic acid amount increase.It should be noted that having following tendency: from being judged as
The form and aspect of the solution of excellent bacterium bed are 2.5YR~10YR, lightness 5, chroma are near 5；From being judged as bad bacterium bed
The form and aspect of solution are 10RP~10R, lightness 2, chroma are near 2.
The superiority and inferiority judgement of fulvic acid solution can consider as shown in following table (table 3).Being judged as excellent solution has not
Easily dissolution humic acid, is easy the tendency of dissolution fulvic acid.Herein, the superiority and inferiority judgement of the bacterium bed in the present invention only should not be solved strictly
The hue range of the Munsell hue ring (40 form and aspect) of following table is released, can also be replaced with substantially equivalent other indexs etc..
[table 3]
Zero: excellent
The moisture content of bacterium bed for Such analysis substantially 50~60 (weight) %.As long as at least 50% or more contains
Water rate can recycle the solution of sufficient amount.Main useless bacterium bed for the analysis should cover upper cover after cultivation to avoid wind
Rain, direct sunlight are taken care of.These useless bacterium beds it is expected that the useless bacterium bed after cultivation is used for solution as early as possible without returning during curing
It receives.Although without being crushed, the case where adapting to the size of solution returnable, dipping in water the case where can also be into
Row is broken.In the present invention, it is above-mentioned it is aqueous be not that only confession under directions is also directed toward dry in the bacterium bed of superior and inferior evaluating contains moisture the case where
The case where giving water in the bacterium bed of dry state.When giving water and bacterium bed being impregnated in a reservoir, do not have for the upper limit of administered dose
It limits.
In addition, the mixed liquor of wood vinegar or bamboo vinegar or wood vinegar and bamboo vinegar can also be given instead of water.As wood
Vinegar liquid does not limit, for example, the substance extracted from Japanese blue Oak Tree (Quercus glauca Thunb) can be enumerated.Make
When with the mixed liquor of wood vinegar or bamboo vinegar or wood vinegar and bamboo vinegar, it can promote the dissolution of fulvic acid compared with water, have
The advantages of obtaining a large amount of fulvic acid with shorter time.The mixed of any mixed proportion can be used in the mixed liquor of wood vinegar and bamboo vinegar
Close object.When useless bacterium bed being immersed in the wood vinegar of pH4.0 herein, at least making pH is judge fulvic acid dissolution the 1st lower than 4.0
Condition.In addition, using following situation as the 2nd condition for judging fulvic acid dissolution: in the Munsell hue of Munsell colour system
It, will wherein for the form and aspect after the form and aspect and fulvic acid dissolution for impregnating the wood vinegar before useless bacterium bed in ring (40 form and aspect)
The form and aspect of any one be set as 0, with rotate clockwise+20, rotation -20 is counterclockwise come when indicating, on the basis of the form and aspect 0, separately
The form and aspect of one are in+4~+6 or -4~-6 hue range.Aforementioned 1st, it is judged as excellent when the 2nd condition is all satisfied.It needs
It is noted that important is the form and aspect of the wood vinegar in repeating test for dissolving out fulvic acid are 7.5R~7.5YR, bright
Degree is 7, chroma 12, the form and aspect of dissolution fulvic acid solution are 10YR~10Y, lightness 2, chroma 1.For wood vinegar
There is no limit pH is preferably 4.0 or less for pH, administered dose, dip time.It should be noted that confirmed the feelings using bamboo vinegar
Condition also be substantially same result the case where using wood vinegar.
Industrial availability
According to the present invention, due to judged based on the appearance of bacterium bed and brittleness culture after mushroom bacterium bed superiority and inferiority, be based on
PH and form and aspect judge the superiority and inferiority of solution, therefore are useful as the technology that can inexpensively and easily obtain a large amount of fulvic acid
's.
Claims (3)
1. a kind of evaluation method of fulvic acid solution superiority and inferiority, which is characterized in that
When extracting fulvic acid solution from the mushroom bacterium bed after culture, the main component of the bacterium bed is sawdust,
The evaluation method includes the following steps:
PH verification step: after the mushroom bacterium bed is enclosed in container, the pH of the solution of dissolution to the container bottom is carried out
Confirmation；
Color verification step: the color of the solution is confirmed；And
Judgment step: based on the pH confirmed in the pH verification step and the color confirmed in the color verification step,
PH be 4.0 or less and color in the Munsell hue ring (40 form and aspect) of Munsell colour system, with it is water-soluble go out when form and aspect
For 2.5YR~10YR, with the mixed liquor of wood vinegar or bamboo vinegar or wood vinegar and bamboo vinegar dissolve out when form and aspect be 10YR~
In the case where 10Y, the solution is judged as excellent.
2. a kind of manufacturing method of fulvic acid solution, which is characterized in that manufacturing fulvic acid solution by the mushroom bacterium bed after cultivating
Manufacturing method in, the main component of the bacterium bed is sawdust,
The manufacturing method includes the following steps:
Step 1 is rapid: will visually confirm the height in the bacterium bed, width, length direction be covered on the whole mycelia and
It is excellent that sensory evaluation is that the high mushroom bacterium bed of brittleness is evaluated as, and is evaluated as in addition to this bad；
Second step: the mushroom bacterium bed is enclosed in container, makes solution dissolution to the container bottom；
Third step: for being be evaluated as excellent mushroom bacterium bed in the step 1 is rapid, to the pH of the dissolution fluid of the second step
Confirmed；And
Step 4 is rapid: for color that the pH that confirms in the third step is 4.0 solution below in the step 1 is rapid
It is evaluated as the color of the solution of the second step of bad mushroom bacterium bed, in the Munsell hue ring (40 of Munsell colour system
Form and aspect) in the form and aspect of any one are set as 0 ,+20 are rotated clockwise relative to the form and aspect, rotation -20 is counterclockwise come when indicating,
The solution form and aspect of another one being in+4~+6 or -4~-6 hue range is judged as excellent,
Excellent solution is judged as fulvic acid solution using in the step 4 is rapid.
3. a kind of manufacturing method of fulvic acid solution, which is characterized in that manufacturing fulvic acid solution by the mushroom bacterium bed after cultivating
Manufacturing method in, the main component of the bacterium bed is sawdust,
The manufacturing method includes the following steps:
Step 1 is rapid: will visually confirm the height in the bacterium bed, width, length direction be covered on the whole mycelia and
It is excellent that sensory evaluation is that the high mushroom bacterium bed of brittleness is evaluated as, and is evaluated as in addition to this bad；
Second step: the mushroom bacterium bed is enclosed together with the mixed liquor of bamboo vinegar with wood vinegar or bamboo vinegar or wood vinegar
In container, make solution dissolution to the container bottom；
Third step: for being be evaluated as excellent mushroom bacterium bed in the step 1 is rapid, to the pH of the dissolution fluid of the second step
Confirmed；And
Step 4 is rapid: being lower than the wood vinegar or the bamboo vinegar or the wood for the pH confirmed in the third step
The color of the solution of the pH of the mixed liquor of vinegar liquid and bamboo vinegar and the wood vinegar or the bamboo vinegar or the wood vinegar with
The color of the mixed liquor of bamboo vinegar sets the form and aspect of any one in the Munsell hue ring (40 form and aspect) of Munsell colour system
It is 0, rotates clockwise+20 relative to the form and aspect, rotation 20 is counterclockwise come when indicating, the form and aspect of another one are in+4~+
Solution in 6 or -4~-6 hue range is judged as excellent,
Excellent solution is judged as fulvic acid solution using in the step 4 is rapid.
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| JP2017224893A JP6331206B1 (en) | 2017-11-22 | 2017-11-22 | Method for evaluating quality of fulvic acid solution and method for producing fulvic acid solution |
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| CN112790064A (en) * | 2021-01-08 | 2021-05-14 | 华南农业大学 | Luminous edible fungus cultivation method based on carbon-based fluorescent nano material |
| JP7043019B1 (en) | 2021-11-14 | 2022-03-29 | 株式会社日本ソフケン | How to release humic substances slowly |
| JP7055311B1 (en) | 2021-11-14 | 2022-04-18 | 株式会社日本ソフケン | Manufacturing method of humic substance solution and humic substance |
| CN114375637A (en) * | 2021-12-27 | 2022-04-22 | 中国农业科学院农业环境与可持续发展研究所 | Wheat seed germination and seedling stage regulation and control method based on fulvic acid spectroscopy characteristics |
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| JP2004269484A (en) * | 2003-03-06 | 2004-09-30 | Fumihiko Yazaki | Method for producing fulvic acid from low-grade coal using high-temperature water under pressure |
| JP6095110B2 (en) * | 2013-02-21 | 2017-03-15 | 孝義 豊田 | Humic acid-derived fulvic acid aqueous solution and method for producing the same |
| JP6026631B1 (en) * | 2015-12-25 | 2016-11-16 | 充日児 ▲高▼味 | Method and apparatus for producing fulvic acid-containing liquid |
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