CN109811020A8 - 利用乌鲁木齐奇异球菌催化合成5-羟甲基糠酸的方法 - Google Patents
利用乌鲁木齐奇异球菌催化合成5-羟甲基糠酸的方法 Download PDFInfo
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- CN109811020A8 CN109811020A8 CN201910210430.9A CN201910210430A CN109811020A8 CN 109811020 A8 CN109811020 A8 CN 109811020A8 CN 201910210430 A CN201910210430 A CN 201910210430A CN 109811020 A8 CN109811020 A8 CN 109811020A8
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- 241001478240 Coccus Species 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 230000002194 synthesizing effect Effects 0.000 title abstract 2
- NOEGNKMFWQHSLB-UHFFFAOYSA-N 5-hydroxymethylfurfural Chemical compound OCC1=CC=C(C=O)O1 NOEGNKMFWQHSLB-UHFFFAOYSA-N 0.000 abstract 2
- RJGBSYZFOCAGQY-UHFFFAOYSA-N hydroxymethylfurfural Natural products COC1=CC=C(C=O)O1 RJGBSYZFOCAGQY-UHFFFAOYSA-N 0.000 abstract 2
- 210000001082 somatic cell Anatomy 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 230000004913 activation Effects 0.000 abstract 1
- 239000011942 biocatalyst Substances 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000003054 catalyst Substances 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
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Abstract
本发明涉及一种利用乌鲁木齐奇异球菌催化合成5-羟甲基糠酸的方法，将乌鲁木齐奇异球菌R12接种于TGY培养基，活化和培养后，收集菌体细胞；将5-羟甲基糠醛加入到pH 6.0～10.0的缓冲液中，混合均匀后，加入收集的菌体细胞，在温度25～60℃下反应3～48h，得到5-羟甲基糠酸。本发明利用的生物催化剂乌鲁木齐奇异球菌对5-羟甲基糠醛具有高耐受性，能够催化高浓度底物选择性氧化合成目标产物，产率达89％以上。本发明方案克服了现有技术中化学催化剂环境不友好的缺点，并且具有底物浓度更高、反应效率更优异，选择性好的特点。
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| CN109811020A CN109811020A (zh) | 2019-05-28 |
| CN109811020A8 true CN109811020A8 (zh) | 2019-06-25 |
| CN109811020B CN109811020B (zh) | 2022-03-29 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP2473494B1 (en) * | 2009-09-02 | 2019-06-12 | Purac Biochem B.V. | Polypeptides having oxidoreductase activity and their uses |
| EP2875142A2 (en) * | 2012-07-20 | 2015-05-27 | Novozymes A/S | Enzymatic oxidation of 5-hydroxymethylfurfural and derivatives thereof |
| CN103626726B (zh) * | 2012-08-23 | 2016-08-03 | 中国科学院大连化学物理研究所 | 一种制备5-羟甲基糠酸和2，5-呋喃二甲酸的方法 |
| CN104846027B (zh) * | 2015-04-30 | 2018-04-27 | 华南理工大学 | 一种酶催化5-羟甲基糠醛合成高附加值衍生物的方法 |
| CN109400557B (zh) * | 2018-11-20 | 2020-08-11 | 贵州理工学院 | 一种利用生物质制备糠酸和5-羟甲基糠酸的方法 |
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