CN109799252A - The automatically receiving device of biological tissue's sequence section - Google Patents
The automatically receiving device of biological tissue's sequence section Download PDFInfo
- Publication number
- CN109799252A CN109799252A CN201811618445.0A CN201811618445A CN109799252A CN 109799252 A CN109799252 A CN 109799252A CN 201811618445 A CN201811618445 A CN 201811618445A CN 109799252 A CN109799252 A CN 109799252A
- Authority
- CN
- China
- Prior art keywords
- slice
- biological tissue
- silicon wafer
- sequence section
- receiving device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000007246 mechanism Effects 0.000 claims abstract description 95
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 91
- 239000010703 silicon Substances 0.000 claims abstract description 91
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims abstract description 90
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 9
- 238000006073 displacement reaction Methods 0.000 claims description 23
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 23
- 239000000919 ceramic Substances 0.000 claims description 19
- 230000005611 electricity Effects 0.000 claims description 4
- 238000004364 calculation method Methods 0.000 claims description 3
- 229910021421 monocrystalline silicon Inorganic materials 0.000 claims description 3
- 238000013519 translation Methods 0.000 claims description 2
- 239000000758 substrate Substances 0.000 abstract description 12
- 238000003384 imaging method Methods 0.000 description 10
- 239000002390 adhesive tape Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 230000008520 organization Effects 0.000 description 6
- 230000033001 locomotion Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013528 artificial neural network Methods 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000009940 knitting Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- UONOETXJSWQNOL-UHFFFAOYSA-N tungsten carbide Chemical compound [W+]#[C-] UONOETXJSWQNOL-UHFFFAOYSA-N 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 230000010148 water-pollination Effects 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to sequence sections to collect field, in particular to a kind of automatically receiving device of biological tissue's sequence section.In order to solve it is existing by silicon wafer as substrate manually collect biological tissue's sequence section in the way of operation difficulty is big, collects the problems such as low efficiency, automatically receiving device proposed by the present invention includes slice sink, is sliced and collects mechanism, positioning system and control system, control system can send a signal to positioning system, and positioning system collects pose of the mechanism relative to slice sink according to the Signal Regulation slice received；Control system can also send a signal to silicon slice rotating mechanism to control the working method after silicon slice rotating mechanism starting/stopping and starting；The biological tissue's sequence section floated on the intracorporal water surface of section water vallecular cavity can be collected under the driving of silicon slice rotating mechanism by collecting silicon wafer.The present invention realizes collecting automatically for biological tissue's sequence section using the excellent silicon wafer of surface characteristic as substrate is collected while guaranteeing to be sliced subsequent image quality.
Description
Technical field
The invention belongs to sequence sections to collect field, in particular to a kind of biological tissue's sequence section collects dress automatically
It sets.
Background technique
Understand in biological tissue the structure of neural network and working principle firstly the need of using scanning electron microscope to life
Object tissue sample carries out high accuracy three-dimensional reconstruction.The main base of three-dimensional reconstruction is carried out to biological organization sample using electron microscope
In ultra-thin sequence section 3 dimension imaging technology, that is, refers to and biological tissue section is cut into ultra-thin sequence section or section, and
It is imaged piecewise using electron microscope.Current ultra-thin sequence section 3 dimension imaging technology be broadly divided into sequence section at
Picture and sequence section are imaged two kinds: (1) sequence cross-sectional imaging mode is to put biological organization sample and ultra-thin section mechanism simultaneously
Enter that electron microscopic sample is intracavitary, be imaged while slice using section of the electron microscope to sample, the shortcomings that this mode
It is damage property slicing mode, secondary imaging can not be carried out to sample, be unfavorable for the development of large-scale reconstruction work；(2) sequence
After sample is cut into sequence section using ultramicrotome by slice imaging mode, while the substrate for using hydrophily to be suitable for is collected
Sequence section is imaged using electron microscope.
Sequence section imaging mode relative to sequence cross-sectional imaging mode be conducive to secondary imaging, it is cheap the advantages that,
But at present for there is also problems on the collecting mode of sequence section.The collection of sequence section mainly has using silicon wafer as substrate
Manually collect and by the automatic slice taking-up machine of substrate of adhesive tape collect two ways automatically: (1) using adhesive tape as substrate from
Dynamic collection mode is able to achieve the automatic of sequence section and collects, however the charge accumulation effects as brought by the non-conductive property of adhesive tape
The image quality of extreme influence slice, while also needing to stick on adhesive tape in subsequent processing on silicon wafer and being sent into electron microscope
Imaging, this causes subsequent operation cumbersome；It (2) is the artificial collecting mode of substrate due to the high conductivity of silicon wafer and excellent using silicon wafer
Good surface characteristic can realize the imaging of good quality to biological tissue's sequence section, however manually collect sectioning difficulty
Greatly, low efficiency is collected.
Guarantee that subsequent image quality is that a needs solve while the automation of sequence section is collected currently, realizing
Problem.Therefore, design it is a kind of using silicon wafer as substrate biological tissue's sequence section automate collection apparatus be of great significance.
Summary of the invention
In order to solve the above problem in the prior art, in order to solve existing manually to collect biology using silicon wafer as substrate
The mode operation difficulty of organization order's slice is big, collects the problems such as low efficiency, and the invention proposes a kind of biological tissue's sequences to cut
The automatically receiving device of piece, biological tissue's sequence section are cut by ultramicrotome, which includes:
It is sliced sink, the slice sink has the cavity for holding pure water, biological tissue's sequence after the ultramicrotome cutting
Slice can float on the intracorporal water surface of the chamber；Slice collects mechanism, the slice collect mechanism include collect silicon wafer and
For driving the silicon slice rotating mechanism for collecting silicon wafer rotation；Positioning system, it is described slice collect mechanism be fixed on it is described fixed
In the system of position, the positioning system is used to adjust the slice and collects pose of the mechanism relative to the slice sink；Control system
System, the control system can send a signal to the positioning system, and the positioning system is according to receiving described in Signal Regulation
Slice collects pose of the mechanism relative to the slice sink；And the control system can also send a signal to the silicon
Piece rotating mechanism is to control the working method after the silicon slice rotating mechanism starting/stopping and starting；It is described to collect silicon wafer energy
It is enough that biological group floated on the intracorporal water surface of section water vallecular cavity is collected under the driving of the silicon slice rotating mechanism
Knit sequence section.
In the preferred embodiment of the automatically receiving device of above-mentioned biological tissue's sequence section, the slice sink includes
Slicer fixed frame, first baffle and second baffle, the slicer fixed mount setting are used in the lateral wall of the slice sink
It is fixed on the slicer of the ultramicrotome in by the slice sink；The first baffle and second baffle setting
In the inner sidewall opposite with the slicer fixed frame of the slice sink, and the first baffle and the second baffle
Between have can by pure water fill gap；Biological tissue's sequence section after the ultramicrotome cutting can be along institute
The water stated in gap is mobile away from the direction of the slicer fixed frame.
In the preferred embodiment of the automatically receiving device of above-mentioned biological tissue's sequence section, the silicon slice rotating mechanism
Including loading stage and the electric machine assembly being fixed on the loading stage and power output component, the electric machine assembly includes electricity
Machine and the motor driver to work for controlling the motor, the motor driver can receive the signal of the control system
The revolving speed after starting/stopping and starting to drive the motor；The output precision include T shape bevel gear and with the T
The bevel gear shaft of shape bevel gear engagement, the T shape bevel gear are connected on the output shaft of the motor, and the bevel gear shaft can revolve
It is connected on the loading stage with turning；The top collected silicon wafer and be sheathed on the bevel gear shaft, the motor pass through described
Silicon wafer rotation is collected described in output precision driving.
In the preferred embodiment of the automatically receiving device of above-mentioned biological tissue's sequence section, the silicon slice rotating mechanism
It further include adjusting gasket and fixing screws, silicon wafer, the adjusting gasket and the fixing screws collected successively are sheathed on institute
The top of bevel gear shaft is stated, the silicon wafer of collecting is fixed on the bevel gear shaft by the adjusting gasket by the fixing screws
Top.
In the preferred embodiment of the automatically receiving device of above-mentioned biological tissue's sequence section, the silicon slice rotating mechanism
It further include motor fixing plate, the motor fixing plate is used to the motor being fixed to the loading stage.
In the preferred embodiment of the automatically receiving device of above-mentioned biological tissue's sequence section, the positioning system includes
Three axle allocation mechanisms and controller, the loading stage are fixed on three axle allocation mechanism, and the controller can receive institute
It states the signal that control system is sent and three axle allocation mechanism is driven according to the signal to adjust the slice and collect mechanism
Pose relative to the slice sink.
In the preferred embodiment of the automatically receiving device of above-mentioned biological tissue's sequence section, three axle allocation mechanism
For three axle allocation mechanism of piezoelectric ceramics, the controller is piezoelectric ceramics controller.
In the preferred embodiment of the automatically receiving device of above-mentioned biological tissue's sequence section, the control system packet
It includes: image acquisition unit, the electronic chart of biological tissue's sequence section after being used to acquire the ultramicrotome cutting in real time
Picture；Processor, the processor can receive the collected electronic image of described image acquiring unit, and by the electronics
Image progress analytical calculation collects revolving speed required for silicon wafer described in obtaining；The processor collects silicon wafer described in can also obtaining
Collection information and receive motor driver feedback the motor work state information；The processor is by basis
The work state information for collecting revolving speed required for silicon wafer, the collection information for collecting silicon wafer and the motor calculates
To control signal be sent to the motor driver with control the electric motor starting/stopping and starting after working method.
In the preferred embodiment of the automatically receiving device of above-mentioned biological tissue's sequence section, the automatically receiving device is also
Including displacement platform, institute's translation stage includes length travel mechanism, pedestal and angle displacement platform, and the pedestal is fixed on described
Length travel mechanism can simultaneously be moved up and down along the length travel mechanism, and the angle displacement platform is fixed on the pedestal,
The positioning system is fixed on the angular displacement platform；It can adjust the height of the positioning system by the pedestal, and because
The slice that this adjusting is fixed in the positioning system collects the height of mechanism；It can be adjusted by the angle displacement platform
The angle of the positioning system, and therefore adjust the angle that the slice being fixed in the positioning system collects mechanism.
In the preferred embodiment of the automatically receiving device of above-mentioned biological tissue's sequence section, the length travel mechanism
For long stroke length travel mechanism comprising pedestal and the first sliding bar and the second sliding bar that are set on the pedestal, institute
The one end for stating pedestal is respectively slidably set on first sliding bar and second sliding bar；And/or it is described
Silicon wafer is collected using the monocrystalline silicon piece Jing Guo laser boring；And/or the silicon wafer of collecting is circle.
With it is existing using silicon wafer compared with the artificial collecting mode of biological tissue's sequence section of substrate, the present invention can be achieved with
Silicon wafer is that the automation of biological tissue's sequence section of substrate is collected, and overcomes operation when manually collecting biological tissue's sequence section difficult
Degree is big, collects the problems such as low efficiency；With it is existing using adhesive tape as biological tissue's sequence collection apparatus of substrate compared with, it is of the invention
Biological tissue's sequence section automation collection apparatus directly collects biological tissue's sequence section on silicon wafer, overcomes subsequent behaviour
The problems such as making low image quality brought by cumbersome, adhesive tape electric conductivity difference.The present invention can be widely used in biological tissue's sequence
Column slice automation is collected in task.
Detailed description of the invention
Fig. 1 is the overall structure diagram of the automatically receiving device of biological tissue's sequence section of the present invention；
Fig. 2 is that the slice of the automatically receiving device of biological tissue's sequence section of the present invention collects the structural schematic diagram of mechanism；
Fig. 3 is the structural schematic diagram of the slice sink of the automatically receiving device of biological tissue's sequence section of the present invention；
Fig. 4 is a kind of control principle structural schematic diagram of the automatically receiving device of biological tissue's sequence section of the present invention.
Drawing reference numeral: 1- slice sink, 10- cavity, 11- first baffle, 12- second baffle, 13- slicer fixed frame,
2- slice collects mechanism, and 21- collects silicon wafer, 22- silicon slice rotating mechanism, 221- loading stage, 222- motor, 223-T shape bevel gear,
224- bevel gear shaft, 225- adjusting gasket, 226- fixing screws, 227- motor fixing plate, 3- positioning system, 4- slicer, 51-
Length travel mechanism, the first sliding bar of 511-, the second sliding bar of 512-, 513- pedestal, 52- pedestal, 53- angle displacement platform.
Specific embodiment
To keep the embodiment of the present invention, technical solution and advantage more obvious, below in conjunction with attached drawing to skill of the invention
Art scheme carries out clear, complete description, it is clear that and the embodiment described is a part of the embodiments of the present invention, rather than all
Embodiment.It will be apparent to a skilled person that these embodiments are used only for explaining technical principle of the invention, and
It is not intended to be limiting protection scope of the present invention.
Biological tissue's sequence section of the invention can be cut by ultramicrotome.It is with reference first to Fig. 1, Fig. 1
The overall structure diagram of the automatically receiving device of biological tissue's sequence section of the present invention.As shown in Figure 1, of the invention biological group
The automatically receiving device for knitting sequence section mainly includes slice sink 1, is sliced and collects mechanism 2, positioning system 3 and control system
(not shown in figure 1).Wherein, there is slice sink 1 cavity 10 for holding pure water (referring to Fig. 3, to show chamber to Fig. 3 cleaning
Body 10), biological tissue's sequence section after ultramicrotome cutting can float on the water surface in cavity 10；Slice collects machine
Structure 2 includes collecting silicon wafer 21 and for driving the silicon slice rotating mechanism 22 for collecting the rotation of silicon wafer 21；Slice is collected mechanism 2 and is fixed on
In positioning system 3, positioning system 3 is used to adjust slice and collects pose of the mechanism 2 relative to slice sink 1；Control system can
Positioning system 3 is sent a signal to, positioning system 3 collects mechanism 2 relative to slice sink 1 according to Signal Regulation slice is received
Pose, control system can also send a signal to silicon slice rotating mechanism 22 with control 22 startings of silicon slice rotating mechanism/stopping and
Working method after starting.It collects silicon wafer 12 and can be collected under the driving of silicon slice rotating mechanism 22 and float on slice sink 1
Biological tissue's sequence section on the water surface in cavity 10.
Automatically receiving device of the invention is described in further detail below with reference to a kind of specific embodiment.
As an example, the Leica EM of the invention that Lai Ka company can be used when preparing biological tissue's sequence section
UC7 ultramicrotome, the ultramicrotome be fixed in matched vibration isolation table, with the collocation of slice sink 1 using preparing biology
Organization order's slice.Facilitate sight equipped with the mobile Leica M80 stereomicroscope system of homocentric formula about the ultramicrotome
Slice environment and slicing processes is surveyed, while arranging in pairs or groups and the available slice environment of Leica IC90E microscope CCD camera and being sliced
The real-time electronic image of journey, is herein no longer described in detail ultramicrotome.Below with reference to Fig. 3 to cutting in the present embodiment
Piece sink 1 is described in further detail.
It is the structural representation of the slice sink of the automatically receiving device of biological tissue's sequence section of the present invention referring to Fig. 3, Fig. 3
Figure.As shown in figure 3, slice sink 1 includes first baffle 11, second baffle 12 and slicer fixed frame 13, slice sink 1 also has
There is the cavity 10 for holding pure water.Wherein, slicer fixed frame 13 is set to the lateral wall of slice sink 1, is used for section water
Slot 1 is fixed on the slicer 4 of ultramicrotome；First baffle 11 and second baffle 12 be set to slice sink 1 with slice
The opposite inner sidewall of knife fixed frame 13, and having between first baffle 11 and second baffle 12 can be by between pure water filling
Gap；The side of water of biological tissue's sequence section in the gap after ultramicrotome cutting away from slicer fixed frame 13
To movement.
Specifically, appropriate high-purity is added in the cavity 10 of slice sink 1 when cutting biological tissue's sequence section
Water makes water surface elevation keep concordant with first baffle 11, second baffle 12, can open after the parameter of ultramicrotome is then arranged
The slice preparation of eozoon organization order, cooperates first baffle 11 and second baffle 12 that can make biological tissue's sequence section of preparation
Advance to perfect order on the water surface between the first baffle 11 and second baffle 12 of slice sink 1.In the present embodiment, slicer 4 can
Using the triangle tungsten carbide steel knife of DDK company.
Referring to Fig. 2, Fig. 2 is that the slice of the automatically receiving device of biological tissue's sequence section of the present invention collects the structure of mechanism
Schematic diagram, for the sake of clarity, in Fig. 2 to collect silicon wafer 21 made cut clearly to show that silicon slice rotating mechanism 22 respectively forms
Partial structure.As shown in Fig. 2, silicon slice rotating mechanism 22 includes loading stage 221 and the motor being fixed on loading stage 221
Component and power output component.Electric machine assembly includes motor 222 and the motor driver for controlling the work of motor 222, motor
Driver can receive the revolving speed after the signal of control system is started with starting/stopping of driving motor 222 and motor 222.
Output precision includes the bevel gear shaft 224 that T shape bevel gear 223 and T shape bevel gear 223 engage, and T shape bevel gear 223 is connected to electricity
On the output shaft of machine 222, bevel gear shaft 224 is rotatably connected on loading stage 221.It collects silicon wafer 21 and is sheathed on bevel gear shaft
224 top, motor 222 are collected silicon wafer 21 by output precision driving and are rotated.
As an example, collecting as shown in Fig. 2, silicon slice rotating mechanism 22 further includes adjusting gasket 225 and fixing screws 226
Silicon wafer 21, adjusting gasket 225 and fixing screws 226 are successively sheathed on the top of bevel gear shaft 224, and fixing screws 226 pass through tune
The top that section gasket 225 will collect silicon wafer 21 and be fixed on bevel gear shaft 224.Further, silicon slice rotating mechanism 22 further includes motor
Fixed plate 227, motor fixing plate 227 are used to motor 222 being fixed to loading stage 221.In the present embodiment, in the present embodiment
Rotating electric machine 222 can using the 3564K024B rotating electric machine and mating planetary reduction box, motor driver of FAULHABER company
Using the MCBL3003/06S motion controller of FAULHABER company.
In a kind of specific embodiment, positioning system 3 includes three axle allocation mechanisms and controller, and loading stage 221 is fixed
On three axle allocation mechanisms, controller can receive the signal that control system is sent and according to signal drive three axle allocation mechanisms with
Adjusting slice is collected mechanism 2 and (is collected relative to the pose of slice sink 1 specifically by silicon slice rotating mechanism 22 is adjusted with adjusting
The angle relative to slice sink 1 of silicon wafer 21).As an example, three axle allocation mechanisms are three axle allocation mechanism of piezoelectric ceramics, control
Device processed is piezoelectric ceramics controller.Specifically, piezoelectric ceramics controller can be fixed on the vibration isolation table that ultramicrotome is fixed
On, piezoelectric ceramics controller also uses the E-665 servo controller of PI Corp. in the present embodiment, and the piezoelectric ceramics controller is simultaneously
Piezoelectric amplifier is configured with for driving three axle allocation mechanism of piezoelectric ceramics.Three axle allocation mechanism of piezoelectric ceramics can be public using PI
The P-611XYZ Three Degree Of Freedom locator of department, the stroke in single-degree-of-freedom are 120 microns, resolution ratio up to 0.2 nanometer, it can be achieved that
Position accurate adjustment to silicon slice rotating mechanism 22.
Referring back to Fig. 1, the automatically receiving device of biological tissue's sequence section of the invention further includes displacement platform, is used
In providing the adjustment of support and preliminary pose for entire automatically receiving device.The displacement platform include length travel mechanism 51,
Pedestal 52 and angle displacement platform 53.Wherein, pedestal 52 is fixed on length travel mechanism 51 and can be along longitudinal direction on displacement mechanism 51
Lower movement, angle displacement platform 53 are fixed on pedestal 52, and positioning system 3 is fixed on (specially piezoelectric ceramics on angular displacement platform 53
Three axle allocation mechanisms are fixed on angle displacement platform 53).Further, three axis localization machine of piezoelectric ceramics can be adjusted by pedestal 52
The height of structure, and therefore adjust the height that the slice being fixed on three axle allocation mechanism of piezoelectric ceramics collects mechanism 2；Pass through angle
Displacement platform 53 can adjust the angle of three axle allocation mechanism of piezoelectric ceramics, and therefore adjust and be fixed on three axis localization machine of piezoelectric ceramics
Slice on structure collects the angle of mechanism 2.
In a kind of specific embodiment, length travel mechanism 51 is long stroke length travel mechanism comprising pedestal
513 respectively slidably cover with the first sliding bar 511 and the second sliding bar 512, one end of pedestal 52 being set on pedestal 513
It is located on the first sliding bar 511 and the second sliding bar 512, as shown in Figure 1, pedestal 52 can be along the first sliding bar 511 and second
Sliding bar 512 slides up and down, and is fixed on the first sliding bar 511 and the suitable position of the second sliding bar 512.As an example,
Long stroke length travel mechanism can use the VAP10 of Thorlabs company, which has indulging for 254mm
To stroke；Pedestal 52 can use Thorlabs company MB1530F optics bread board；Angle displacement platform 53 can use
Thorlabs company GNL10 angle displacement platform, rotation angle are ± 10 °.
So, automatically receiving device of the invention can by displacement platform and three axis fine-tuned system of piezoelectric ceramics come
Position and the angle for collecting silicon wafer 21 are adjusted, collects life so as to collect silicon wafer 21 and extend into slice sink 1 with suitable pose
Object organization order slice.Preferably, collecting silicon wafer 21 can also further be incited somebody to action using the monocrystalline silicon piece Jing Guo laser boring
Silicon wafer 21 is collected to be provided in round.
In a kind of possible embodiment, control system includes image acquisition unit and processor.Wherein, image obtains
Electronic image of the unit for biological tissue's sequence section after acquiring ultramicrotome cutting in real time；Processor can receive figure
It is obtained required for collecting silicon wafer 21 as the collected electronic image of acquiring unit, and by carrying out analytical calculation to electronic image
Revolving speed；And processor can also obtain the collection information for collecting silicon wafer 21 and receive the motor 222 of motor driver feedback
Work state information, and the work of the revolving speed according to required for collecting silicon wafer 21, the collection information and motor 222 of collecting silicon wafer 21
The control signal being calculated as status information is sent to motor driver to control 222 startings of motor/stopping and motor
Working method after 222 startings.As an example, image acquisition unit can be microscope CCD camera, the microscope CCD camera
The a part that can be used as ultramicrotome, is also possible to independently of ultramicrotome.
Below with reference to the working principle of Fig. 4 the present invention will be described in detail automatically receiving device.
Referring to Fig. 4, Fig. 4 is that a kind of control principle structure of the automatically receiving device of biological tissue's sequence section of the present invention is shown
It is intended to.As shown in figure 4, processor is by carrying out the collected electronic image of microscope CCD camera (i.e. image acquisition unit)
Analysis perceives fltting speed of biological tissue's sequence section in slice sink 1 to resolve the suitable rotational speed for collecting silicon wafer 21, and
And processor can also perceive the degree of collecting for collecting silicon wafer 21, while receive the work for the motor 222 that motor driver transmits
The control instruction being finally calculated is transmitted to motor driver to control motor 222 and work by state.Specifically, motor drives
Dynamic device receives the control instruction of processor, driving motor 222 after control instruction is amplified, while acquiring the work shape of motor 222
State feeds back to processor.
Referring to Fig.1-4, the working principle of automatically receiving device of the invention are as follows: be first loaded into slice sink 1 ultra-thin
Biological tissue's sequence section is used to prepare on the knife platform of slicer.Recycle displacement platform to the first step of automation collection apparatus
After appearance is adjusted, makes to collect silicon wafer 21 and protrude into slice sink 1, then processor is by sending control instruction to piezoelectric ceramics
Controller makes it control the movement of three axle allocation mechanism of Piezoelectric Ceramic and collects silicon wafer 12 to suitable pose with accurate adjusting.
Further, appropriate high-purity water is added in slice sink 1 keeps water surface elevation and the first baffle of slice sink 1, second
Baffle is concordant, starts the preparation of biological tissue's sequence section after ultramicrotome parameter is arranged, is adopted in real time by microscope CCD camera
Electronic image is simultaneously fed back to processor by the electronic image of collection biological tissue's sequence section preparation, and processor passes through to electronic image
Information is analyzed to obtain fltting speed of biological tissue's sequence section in slice sink 1 and collects the suitable of silicon wafer 21 to resolve
Revolving speed, and at the same time analyzing the degree of collecting for collecting silicon wafer 21, the control that resolving obtains sequence section automatically receiving device refers to
It enables, exports control instruction to motor driver using processor and control the electricity in biological tissue's sequence section automatically receiving device
The start and stop and rotation speed of machine 222 collect the collecting automatically to biological tissue's sequence section of silicon wafer 21 to realize.
So far, it has been combined preferred embodiment shown in the drawings and describes technical solution of the present invention, still, this field
Technical staff is it is easily understood that protection scope of the present invention is expressly not limited to these specific embodiments.Without departing from this
Under the premise of the principle of invention, those skilled in the art can make equivalent change or replacement to the relevant technologies feature, these
Technical solution after change or replacement will fall within the scope of protection of the present invention.
Claims (10)
1. a kind of automatically receiving device of biological tissue's sequence section, which is cut by ultramicrotome
It forms, which is characterized in that the automatically receiving device includes:
It is sliced sink, the slice sink has the cavity for holding pure water, the biological tissue after the ultramicrotome cutting
Sequence section can float on the intracorporal water surface of the chamber；
Slice collects mechanism, and it includes collecting silicon wafer and for driving the silicon wafer for collecting silicon wafer rotation that the slice, which collects mechanism,
Rotating mechanism；
Positioning system, the slice are collected mechanism and are fixed in the positioning system, and the positioning system is for adjusting described cut
Piece collects pose of the mechanism relative to the slice sink；
Control system, the control system can send a signal to the positioning system, and the positioning system is according to receiving letter
Number adjust it is described slice collect mechanism relative to it is described slice sink pose；And the control system can also send letter
Number to the silicon slice rotating mechanism with control the silicon slice rotating mechanism starting/stopping and starting after working method；
It is described collect silicon wafer and can be collected under the driving of the silicon slice rotating mechanism described float on the section water slot cavity body
Biological tissue's sequence section on the interior water surface.
2. the automatically receiving device of biological tissue's sequence section according to claim 1, which is characterized in that the section water
Slot includes slicer fixed frame, first baffle and second baffle,
The slicer fixed mount setting is described super for the slice sink to be fixed in the lateral wall of the slice sink
On the slicer of slice machine；
The first baffle and the second baffle be set to it is described slice sink it is opposite with the slicer fixed frame in
Side wall, and there is the gap that can be filled by pure water between the first baffle and the second baffle；
Biological tissue's sequence section after the ultramicrotome cutting can be cut along the water in the gap away from described
The direction of piece knife fixed frame is mobile.
3. the automatically receiving device of biological tissue's sequence section according to claim 2, which is characterized in that the silicon wafer rotation
Rotation mechanism includes loading stage and the electric machine assembly being fixed on the loading stage and power output component,
The electric machine assembly includes motor and the motor driver for controlling the motor work, and the motor driver can
Revolving speed after the signal of the control system is received to drive the motor starting/stopping and starting；
The output precision includes T shape bevel gear and the bevel gear shaft that engages with the T shape bevel gear, and the T shape bevel gear connects
It connects on the output shaft of the motor, the bevel gear shaft is rotatably connected on the loading stage；
The top collected silicon wafer and be sheathed on the bevel gear shaft, the motor described in output precision driving by collecting
Silicon wafer rotation.
4. the automatically receiving device of biological tissue's sequence section according to claim 3, which is characterized in that the silicon wafer rotation
Rotation mechanism further includes adjusting gasket and fixing screws,
The top collected silicon wafer, the adjusting gasket and the fixing screws and be successively sheathed on the bevel gear shaft, it is described
Fixing screws are by the adjusting gasket by the top collected silicon wafer and be fixed on the bevel gear shaft.
5. the automatically receiving device of biological tissue's sequence section according to claim 4, which is characterized in that the silicon wafer rotation
Rotation mechanism further includes motor fixing plate,
The motor fixing plate is used to the motor being fixed to the loading stage.
6. the automatically receiving device of biological tissue's sequence section according to claim 3, which is characterized in that the positioning system
System includes three axle allocation mechanisms and controller,
The loading stage is fixed on three axle allocation mechanism, and the controller can receive the letter that the control system is sent
Number and three axle allocation mechanism is driven according to the signal to adjust the slice and collect mechanism relative to the slice sink
Pose.
7. the automatically receiving device of biological tissue's sequence section according to claim 6, which is characterized in that three axis is fixed
Position mechanism is three axle allocation mechanism of piezoelectric ceramics, and the controller is piezoelectric ceramics controller.
8. the automatically receiving device of biological tissue's sequence section according to claim 6, which is characterized in that the control system
System includes:
Image acquisition unit, the electronic chart of biological tissue's sequence section after being used to acquire the ultramicrotome cutting in real time
Picture；
Processor, the processor can receive the collected electronic image of described image acquiring unit, and by the electricity
Subgraph progress analytical calculation collects revolving speed required for silicon wafer described in obtaining；
The processor can also obtain the collection information for collecting silicon wafer and receive the institute of the motor driver feedback
State the work state information of motor；
The processor will collect revolving speed required for silicon wafer, the collection information for collecting silicon wafer and the motor according to
The control signal that is calculated of work state information be sent to the motor driver with control the electric motor starting/stopping,
And the working method after starting.
9. the automatically receiving device of biological tissue's sequence section according to any one of claim 1 to 8, feature exist
In the automatically receiving device further includes displacement platform, and institute's translation stage includes length travel mechanism, pedestal and angle displacement
Platform,
The pedestal is fixed on the length travel mechanism and can move up and down along the length travel mechanism, the angle position
Moving stage is fixed on the pedestal, and the positioning system is fixed on the angular displacement platform；
The height of the positioning system can be adjusted by the pedestal, and therefore adjusts the institute being fixed in the positioning system
State the height that slice collects mechanism；
The angle of the positioning system can be adjusted by the angle displacement platform, and is therefore adjusted and be fixed on the positioning system
On the slice collect the angle of mechanism.
10. the automatically receiving device of biological tissue's sequence section according to claim 9, which is characterized in that the longitudinal direction
Displacement mechanism is long stroke length travel mechanism comprising pedestal and the first sliding bar being set on the pedestal and second are slided
Lever,
One end of the pedestal is respectively slidably set on first sliding bar and second sliding bar；
And/or the silicon wafer of collecting is using the monocrystalline silicon piece Jing Guo laser boring；
And/or the silicon wafer of collecting is circle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811618445.0A CN109799252A (en) | 2018-12-28 | 2018-12-28 | The automatically receiving device of biological tissue's sequence section |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811618445.0A CN109799252A (en) | 2018-12-28 | 2018-12-28 | The automatically receiving device of biological tissue's sequence section |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109799252A true CN109799252A (en) | 2019-05-24 |
Family
ID=66557976
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811618445.0A Pending CN109799252A (en) | 2018-12-28 | 2018-12-28 | The automatically receiving device of biological tissue's sequence section |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109799252A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5906148A (en) * | 1997-01-10 | 1999-05-25 | Chuo Precision Industrial Co., Ltd. | Microtome having vibration dampening and micro-feeding mechanism |
| CN102689678A (en) * | 2012-06-20 | 2012-09-26 | 徐浩钟 | Guide floater collection boat |
| CN103103970A (en) * | 2012-12-08 | 2013-05-15 | 重庆示展科技发展中心 | Floating type drifter guide cleaning station |
| CN103175780A (en) * | 2013-03-20 | 2013-06-26 | 上海交通大学 | Automated imaging system of large-size serial section of biological tissue |
| CN107505339A (en) * | 2017-07-21 | 2017-12-22 | 中国科学院生物物理研究所 | A kind of preparation of Serial ultrathin sections and automatic collection method |
| CN107574806A (en) * | 2017-09-28 | 2018-01-12 | 广西大学 | A kind of mobile method and device for collecting illiquidity floater |
| CN207864220U (en) * | 2018-02-05 | 2018-09-14 | 浙江科力风机有限公司 | A kind of wind turbine rotating platform |
| CN108747691A (en) * | 2018-06-14 | 2018-11-06 | 德清阳瑞光学科技有限公司 | Eyeglass chamfered edge grinder |
| CN208214571U (en) * | 2018-03-12 | 2018-12-11 | 苏州富润泽激光科技有限公司 | A kind of laser-beam welding machine |
-
2018
- 2018-12-28 CN CN201811618445.0A patent/CN109799252A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5906148A (en) * | 1997-01-10 | 1999-05-25 | Chuo Precision Industrial Co., Ltd. | Microtome having vibration dampening and micro-feeding mechanism |
| CN102689678A (en) * | 2012-06-20 | 2012-09-26 | 徐浩钟 | Guide floater collection boat |
| CN103103970A (en) * | 2012-12-08 | 2013-05-15 | 重庆示展科技发展中心 | Floating type drifter guide cleaning station |
| CN103175780A (en) * | 2013-03-20 | 2013-06-26 | 上海交通大学 | Automated imaging system of large-size serial section of biological tissue |
| CN107505339A (en) * | 2017-07-21 | 2017-12-22 | 中国科学院生物物理研究所 | A kind of preparation of Serial ultrathin sections and automatic collection method |
| CN107574806A (en) * | 2017-09-28 | 2018-01-12 | 广西大学 | A kind of mobile method and device for collecting illiquidity floater |
| CN207864220U (en) * | 2018-02-05 | 2018-09-14 | 浙江科力风机有限公司 | A kind of wind turbine rotating platform |
| CN208214571U (en) * | 2018-03-12 | 2018-12-11 | 苏州富润泽激光科技有限公司 | A kind of laser-beam welding machine |
| CN108747691A (en) * | 2018-06-14 | 2018-11-06 | 德清阳瑞光学科技有限公司 | Eyeglass chamfered edge grinder |
Non-Patent Citations (1)
| Title |
|---|
| LIU W, CHENG L, MENG D.: "Brain slices microscopic detection using simplified SSD with Cycle-GAN data augmentation", 《INTERNATIONAL CONFERENCE ON NEURAL INFORMATION PROCESSING》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208459680U (en) | A kind of 3D microscope | |
| CN103399398B (en) | Automatically-focused microscope | |
| JP4297736B2 (en) | Focused ion beam device | |
| EP3063523A1 (en) | Motion strategies for scanning microscope imaging | |
| CN109799252A (en) | The automatically receiving device of biological tissue's sequence section | |
| CN108593648A (en) | A kind of 3D micro-structure observation device and its observation procedure | |
| CN209784016U (en) | Blood smear preparation instrument | |
| CN216144726U (en) | Real-time fluorescence monitoring freezing focusing ion beam processing device | |
| JP2022099272A (en) | Ultramicrotome for three-dimensional imaging | |
| CN112763498A (en) | Micro milling cutter quality detection device and method | |
| CN114603611B (en) | Accurate paraffin section device and use method thereof | |
| CN113686557B (en) | Copper deposition backlight slice preparation method and detection method | |
| CN213351033U (en) | Hydraulic cylinder machining platform for metallurgical equipment | |
| CN215912151U (en) | Remote transmission device for digital pathological section | |
| CN212009140U (en) | Electric objective table suitable for micro-scanner and micro-scanner | |
| CN211477663U (en) | Sheet arranging machine and sheet dragging and arranging machine for slicing and displaying | |
| CN211477660U (en) | Pathological microtome | |
| CN211477662U (en) | Pathological section developing machine | |
| CN113670964A (en) | Real-time fluorescence monitoring freezing focusing ion beam processing device and method | |
| EP3009873B1 (en) | Sample retaining member insertion-removal mechanism and image acquisition device | |
| CN112068302A (en) | Pathological section microscopic detection device capable of reducing pollution | |
| CN218002943U (en) | Pathologic sampling device for hard texture samples | |
| CN220360462U (en) | Microscope cantilever convenient to adjust and used for medical operation | |
| CN220297177U (en) | Multifunctional pathological section knife | |
| CN220893798U (en) | Slicer convenient to observe |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190524 |