CN109797179A - A kind of environment-friendly preparation method thereof of yeast dextran - Google Patents
A kind of environment-friendly preparation method thereof of yeast dextran Download PDFInfo
- Publication number
- CN109797179A CN109797179A CN201910216107.2A CN201910216107A CN109797179A CN 109797179 A CN109797179 A CN 109797179A CN 201910216107 A CN201910216107 A CN 201910216107A CN 109797179 A CN109797179 A CN 109797179A
- Authority
- CN
- China
- Prior art keywords
- added
- tourmaline
- temperature
- yeast
- environment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to dextran techniques fields, more particularly, to a kind of environment-friendly preparation method thereof of yeast dextran: step 1, common yeast cell wall is added in distilled water, it stirs evenly to form suspension, then heated at constant temperature 2-4h is added tourmaline and hydrogen peroxide Sealed steam cooking 2-4h is added, keeps the temperature 2-6h, tourmaline is taken out, obtains sediment after centrifugation；Step 2, sediment will be added into distilled water and is stirred evenly, dispersing agent is added and heating stirring forms suspension, it is 9-11 that lye, which is then added, and is adjusted to pH, obtains alkaline suspension；Step 3, protease and tourmaline are added into alkaline suspension, constant temperature digests 4-6h, is centrifuged after taking out tourmaline, and washing obtains insoluble matter；Step 4, microwave reaction 2-5h is added into acetone in insoluble matter, freeze-drying obtains yeast dextran powder after filtering.The present invention as a raw material for production, using tourmaline as cocatalyst and auxiliary oxidizing agent, greatly improves the yield of yeast dextran with yeast cell wall.
Description
Technical field
The invention belongs to dextran techniques fields, more particularly, to a kind of environment-friendly preparation method thereof of yeast dextran.
Background technique
Yeast callose (abbreviation yeast beta-dextran or yeast dextran) is that one kind possesses good physiology function at present
The immunomodulator (nonspecific immunity strengthening agent) of energy has strengthen immunity, antitumor, antibacterial anti-inflammatory, anti-oxidant, promotion
The physiological functions such as wound healing, anti-radiation, reduction blood lipid, but its not soluble in water and most other solvent, are only very slightly soluble in
Dimethyl sulfoxide, this severely limits the applications of yeast dextran.
In order to improve the solubility of yeast dextran, domestic and foreign scholars have carried out modification and study on the modification to it, including change
Method and physical method.Main chemical modification method has phosphorylation, carboxy methylation, Sulfation and sulfonates modification, passes through
These polar groups are connected on glucan to increase its solubility.But this kind of reaction usually will be homogeneous in liquid or non-equal
It is carried out in phase system, needs to consume a large amount of solvent, even dangerous organic solvent and acid, alkali etc. are not only at high cost, dirty
Dye is serious, it is also possible to cause security risk.Main physical method is ultrasonic depolymerization and oxidation degradation method, by reducing glucan
Molecular weight increase its solubility.But ultrasonic depolymerization can only reduce the molecular weight of glucan to a limit constant value, and
Oxidative degradation can then introduce the impurity such as oxidant in glucan.
Summary of the invention
For the problems of the prior art, the present invention provides a kind of environment-friendly preparation method thereof of yeast dextran, thin with yeast
Cell wall as a raw material for production, using tourmaline as cocatalyst and auxiliary oxidizing agent, greatly improves the production of yeast dextran
Rate.
To realize the above technical purpose, the technical scheme is that
A kind of environment-friendly preparation method thereof of yeast dextran, includes the following steps:
Step 1, by common yeast cell wall be added distilled water in, stir evenly to form suspension, heated at constant temperature 2-4h, then plus
Enter tourmaline and hydrogen peroxide Sealed steam cooking 2-4h is added, keep the temperature 2-6h, takes out tourmaline, obtain sediment after centrifugation；
Step 2, sediment will be added into distilled water and is stirred evenly, dispersing agent is added and heating stirring forms suspension, so
It is 9-11 that lye is added afterwards and is adjusted to pH, obtains alkaline suspension；
Step 3, protease and tourmaline are added into alkaline suspension, constant temperature digests 4-6h, is centrifuged after taking out tourmaline, water
It washes to obtain insoluble matter；
Step 4, microwave reaction 2-5h is added into acetone in insoluble matter, freeze-drying obtains yeast dextran powder after filtering.
Concentration of the yeast cell wall in distilled water in the step 1 is 15-45g/L, the mixing speed stirred evenly
For 500-1000r/min, the temperature of heated at constant temperature is 90-100 DEG C.
The quality of tourmaline in the step 1 is common yeast cell wall quality 2-10%, the additional amount of the hydrogen peroxide
It is the 110-150% of tourmaline quality, the temperature of the Sealed steam cooking is 120-150 DEG C, and holding temperature is 80-90 DEG C.
Concentration of the sediment in distilled water in the step 2 is 100-190g/L, and the dispersing agent uses polyethylene
Pyrrolidones, additional amount are the 2-5% of sediment quality, and the temperature of the heating stirring is 50-70 DEG C, mixing speed 100-
300r/min, the lye use sodium hydroxide solution.
Protease additional amount in the step 3 is the 0.1-0.3% of sediment quality, and the tourmaline is using in step 1
Tourmaline.
The temperature of constant temperature enzymatic hydrolysis in the step 3 is 30-40 DEG C.
The concentration of insoluble matter in acetone in the step 4 is 10-30g/L, and the temperature of microwave reaction is 20-30 DEG C,
Power is 300-500W.
Freeze-drying in the step 4 includes -70 DEG C of precoolings 22h, -60 DEG C of freeze-drying 6-10h.
Common yeast cell wall is added distilled water and forms suspension by step 1, and the mode of heated at constant temperature carries out high temperature extraction,
The hole of cell wall is opened；The pyroelectricity of tourmaline is sufficiently used in the cooperation of tourmaline and hydrogen peroxide simultaneously, generates anion,
The peroxy-radical of anion and hydrogen peroxide itself forms cooperation, can sufficiently open cell wall, is significantly promoted and extracts yield.
Sediment is added to distilled water and is added to be formed in dispersing agent and is sufficiently stirred by step 2, and in alkaline condition shape
At alkaline suspension, the use of dispersing agent can further promote the dispersion of sediment.
The use of protease and tourmaline is carried out constant temperature enzyme digestion reaction, tourmaline makes full use of its thermoelectricity at this time by step 3
Property the anion that generates, the active group of stimulates the protein enzyme effectively improves the progress of enzyme digestion reaction, while anion is to heavy
Activity enzymatic hydrolysis group in starch plays facilitation.
Acetone is added in insoluble matter by step 4, and is effectively mentioned the kinetic energy of impurity inside insoluble matter using microwave reaction
Rise removal of impurity.
From the above, it can be seen that the present invention has following advantages:
1. the present invention with yeast cell wall as a raw material for production, using tourmaline as cocatalyst and auxiliary oxidizing agent, significantly
Improve the yield of yeast dextran.
2. the present invention is matched with tourmaline with hydrogen peroxide, yeast cell wall is opened in a manner of boiling, it can be effective
The release for promoting yeast dextran, solves the problems, such as that the release rate of traditional extraction technique is low.
3. the present invention promotes the enzymatic hydrolysis of protease to imitate using tourmaline as cocatalyst by the pyroelectricity of tourmaline
Rate not only improves enzymolysis speed, but also improves enzymatic hydrolysis depth.
4. the impurity that the present invention is promoted by the way of microwave reaction in insoluble matter quickly dissolves, reduce the residual of impurity
It stays.
Specific embodiment
The present invention will be described in detail in conjunction with the embodiments, but does not do any restriction to claim of the invention.
Embodiment 1
A kind of environment-friendly preparation method thereof of yeast dextran, includes the following steps:
Step 1, common yeast cell wall is added in distilled water, stirs evenly to form suspension, then heated at constant temperature 2h is added
Simultaneously hydrogen peroxide Sealed steam cooking 2h is added in tourmaline, keeps the temperature 2h, takes out tourmaline, obtains sediment after centrifugation；
Step 2, sediment will be added into distilled water and is stirred evenly, dispersing agent is added and heating stirring forms suspension, so
It is 9 that lye is added afterwards and is adjusted to pH, obtains alkaline suspension；
Step 3, protease and tourmaline are added into alkaline suspension, constant temperature digests 4h, is centrifuged after taking out tourmaline, washes
Obtain insoluble matter；
Step 4, microwave reaction 2h is added into acetone in insoluble matter, freeze-drying obtains yeast dextran powder after filtering.
Concentration of the yeast cell wall in distilled water in the step 1 is 15g/L, and the mixing speed stirred evenly is
500r/min, the temperature of heated at constant temperature are 90 DEG C.
The quality of tourmaline in the step 1 is common yeast cell wall quality 2%, and the additional amount of the hydrogen peroxide is
The 110% of tourmaline quality, the temperature of the Sealed steam cooking are 120 DEG C, and holding temperature is 80 DEG C.
Concentration of the sediment in distilled water in the step 2 is 100g/L, and the dispersing agent uses polyvinyl pyrrole
Alkanone, additional amount are the 2% of sediment quality, and the temperature of the heating stirring is 50 DEG C, mixing speed 100r/min, described
Lye uses sodium hydroxide solution.
Protease additional amount in the step 3 is the 0.1% of sediment quality, and the tourmaline is using the electricity in step 1
Gas stone.
The temperature of constant temperature enzymatic hydrolysis in the step 3 is 30 DEG C.
The concentration of insoluble matter in acetone in the step 4 is 10g/L, and the temperature of microwave reaction is 20 DEG C, and power is
300W。
Freeze-drying in the step 4 includes -70 DEG C of precoolings 22h, -60 DEG C of freeze-drying 6h.
Embodiment 2
A kind of environment-friendly preparation method thereof of yeast dextran, includes the following steps:
Step 1, common yeast cell wall is added in distilled water, stirs evenly to form suspension, then heated at constant temperature 4h is added
Simultaneously hydrogen peroxide Sealed steam cooking 4h is added in tourmaline, keeps the temperature 6h, takes out tourmaline, obtains sediment after centrifugation；
Step 2, sediment will be added into distilled water and is stirred evenly, dispersing agent is added and heating stirring forms suspension, so
It is 11 that lye is added afterwards and is adjusted to pH, obtains alkaline suspension；
Step 3, protease and tourmaline are added into alkaline suspension, constant temperature digests 6h, is centrifuged after taking out tourmaline, washes
Obtain insoluble matter；
Step 4, microwave reaction 5h is added into acetone in insoluble matter, freeze-drying obtains yeast dextran powder after filtering.
Concentration of the yeast cell wall in distilled water in the step 1 is 45g/L, and the mixing speed stirred evenly is
1000r/min, the temperature of heated at constant temperature are 100 DEG C.
The quality of tourmaline in the step 1 is common yeast cell wall quality 10%, and the additional amount of the hydrogen peroxide is
The 150% of tourmaline quality, the temperature of the Sealed steam cooking are 150 DEG C, and holding temperature is 90 DEG C.
Concentration of the sediment in distilled water in the step 2 is 190g/L, and the dispersing agent uses polyvinyl pyrrole
Alkanone, additional amount are the 5% of sediment quality, and the temperature of the heating stirring is 70 DEG C, mixing speed 300r/min, described
Lye uses sodium hydroxide solution.
Protease additional amount in the step 3 is the 0.3% of sediment quality, and the tourmaline is using the electricity in step 1
Gas stone.
The temperature of constant temperature enzymatic hydrolysis in the step 3 is 40 DEG C.
The concentration of insoluble matter in acetone in the step 4 is 30g/L, and the temperature of microwave reaction is 30 DEG C, and power is
500W。
Freeze-drying in the step 4 includes -70 DEG C of precoolings 22h, -60 DEG C of freeze-drying 10h.
Embodiment 3
A kind of environment-friendly preparation method thereof of yeast dextran, includes the following steps:
Step 1, common yeast cell wall is added in distilled water, stirs evenly to form suspension, then heated at constant temperature 3h is added
Simultaneously hydrogen peroxide Sealed steam cooking 3h is added in tourmaline, keeps the temperature 4h, takes out tourmaline, obtains sediment after centrifugation；
Step 2, sediment will be added into distilled water and is stirred evenly, dispersing agent is added and heating stirring forms suspension, so
It is 10 that lye is added afterwards and is adjusted to pH, obtains alkaline suspension；
Step 3, protease and tourmaline are added into alkaline suspension, constant temperature digests 5h, is centrifuged after taking out tourmaline, washes
Obtain insoluble matter；
Step 4, microwave reaction 4h is added into acetone in insoluble matter, freeze-drying obtains yeast dextran powder after filtering.
Concentration of the yeast cell wall in distilled water in the step 1 is 30g/L, and the mixing speed stirred evenly is
800r/min, the temperature of heated at constant temperature are 100 DEG C.
The quality of tourmaline in the step 1 is the 8% of common yeast cell wall quality, the additional amount of the hydrogen peroxide
It is the 130% of tourmaline quality, the temperature of the Sealed steam cooking is 140 DEG C, and holding temperature is 85 DEG C.
Concentration of the sediment in distilled water in the step 2 is 160g/L, and the dispersing agent uses polyvinyl pyrrole
Alkanone, additional amount are the 4% of sediment quality, and the temperature of the heating stirring is 60 DEG C, mixing speed 200r/min, described
Lye uses sodium hydroxide solution.
Protease additional amount in the step 3 is the 0.2% of sediment quality, and the tourmaline is using the electricity in step 1
Gas stone.
The temperature of constant temperature enzymatic hydrolysis in the step 3 is 35 DEG C.
The concentration of insoluble matter in acetone in the step 4 is 20g/L, and the temperature of microwave reaction is 25 DEG C, and power is
400W。
Freeze-drying in the step 4 includes -70 DEG C of precoolings 22h, -60 DEG C of freeze-drying 8h.
Performance detection
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Condition | Pale yellow powder | Pale yellow powder | Pale yellow powder |
| Beta-dextran content | 80% | 85% | 83% |
In conclusion the invention has the following advantages that
1. the present invention with yeast cell wall as a raw material for production, using tourmaline as cocatalyst and auxiliary oxidizing agent, significantly
Improve the yield of yeast dextran.
2. the present invention is matched with tourmaline with hydrogen peroxide, yeast cell wall is opened in a manner of boiling, it can be effective
The release for promoting yeast dextran, solves the problems, such as that the release rate of traditional extraction technique is low.
3. the present invention promotes the enzymatic hydrolysis of protease to imitate using tourmaline as cocatalyst by the pyroelectricity of tourmaline
Rate not only improves enzymolysis speed, but also improves enzymatic hydrolysis depth.
4. the impurity that the present invention is promoted by the way of microwave reaction in insoluble matter quickly dissolves, reduce the residual of impurity
It stays.
It is understood that being merely to illustrate the present invention above with respect to specific descriptions of the invention and being not limited to this
Technical solution described in inventive embodiments.Those skilled in the art should understand that still can be carried out to the present invention
Modification or equivalent replacement, to reach identical technical effect；As long as meet use needs, all protection scope of the present invention it
It is interior.
Claims (8)
1. a kind of environment-friendly preparation method thereof of yeast dextran, characterized by the following steps:
Step 1, by common yeast cell wall be added distilled water in, stir evenly to form suspension, heated at constant temperature 2-4h, then plus
Enter tourmaline and hydrogen peroxide Sealed steam cooking 2-4h is added, keep the temperature 2-6h, takes out tourmaline, obtain sediment after centrifugation；
Step 2, sediment will be added into distilled water and is stirred evenly, dispersing agent is added and heating stirring forms suspension, so
It is 9-11 that lye is added afterwards and is adjusted to pH, obtains alkaline suspension；
Step 3, protease and tourmaline are added into alkaline suspension, constant temperature digests 4-6h, is centrifuged after taking out tourmaline, water
It washes to obtain insoluble matter；
Step 4, microwave reaction 2-5h is added into acetone in insoluble matter, freeze-drying obtains yeast dextran powder after filtering.
2. the environment-friendly preparation method thereof of yeast dextran according to claim 1, it is characterised in that: the ferment in the step 1
Concentration of the mother cell wall in distilled water is 15-45g/L, and the mixing speed stirred evenly is 500-1000r/min, heated at constant temperature
Temperature be 90-100 DEG C.
3. the environment-friendly preparation method thereof of yeast dextran according to claim 1, it is characterised in that: the electricity in the step 1
The quality of gas stone is common yeast cell wall quality 2-10%, and the additional amount of the hydrogen peroxide is the 110- of tourmaline quality
150%, the temperature of the Sealed steam cooking is 120-150 DEG C, and holding temperature is 80-90 DEG C.
4. the environment-friendly preparation method thereof of yeast dextran according to claim 1, it is characterised in that: heavy in the step 2
Concentration of the starch in distilled water is 100-190g/L, and the dispersing agent uses polyvinylpyrrolidone, and additional amount is sediment
The 2-5% of quality, the temperature of the heating stirring are 50-70 DEG C, mixing speed 100-300r/min, and the lye uses hydrogen
Sodium hydroxide solution.
5. the environment-friendly preparation method thereof of yeast dextran according to claim 1, it is characterised in that: the egg in the step 3
White enzyme additional amount is the 0.1-0.3% of sediment quality, and the tourmaline is using the tourmaline in step 1.
6. the environment-friendly preparation method thereof of yeast dextran according to claim 1, it is characterised in that: the perseverance in the step 3
The temperature of temperature enzymatic hydrolysis is 30-40 DEG C.
7. the environment-friendly preparation method thereof of yeast dextran according to claim 1, it is characterised in that: in the step 4 not
The concentration of molten object in acetone is 10-30g/L, and the temperature of microwave reaction is 20-30 DEG C, power 300-500W.
8. the environment-friendly preparation method thereof of yeast dextran according to claim 1, it is characterised in that: cold in the step 4
Being lyophilized dry includes -70 DEG C of precoolings 22h, -60 DEG C of freeze-drying 6-10h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910216107.2A CN109797179B (en) | 2019-03-20 | 2019-03-20 | Green preparation method of yeast glucan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910216107.2A CN109797179B (en) | 2019-03-20 | 2019-03-20 | Green preparation method of yeast glucan |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109797179A true CN109797179A (en) | 2019-05-24 |
| CN109797179B CN109797179B (en) | 2021-02-09 |
Family
ID=66563844
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910216107.2A Active CN109797179B (en) | 2019-03-20 | 2019-03-20 | Green preparation method of yeast glucan |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109797179B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113121719A (en) * | 2021-06-03 | 2021-07-16 | 南京先达医药科技有限公司 | Extraction process of yeast-beta-glucan |
| CN114163548A (en) * | 2021-12-13 | 2022-03-11 | 常州市第二人民医院 | Production process of yeast glucan |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101012468A (en) * | 2007-02-07 | 2007-08-08 | 天津科技大学 | Yeast glucans extraction process |
| CN101117357A (en) * | 2007-09-17 | 2008-02-06 | 中国农业大学 | Method for preparing water-soluble beta-1,3/1,6-dextran |
-
2019
- 2019-03-20 CN CN201910216107.2A patent/CN109797179B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101012468A (en) * | 2007-02-07 | 2007-08-08 | 天津科技大学 | Yeast glucans extraction process |
| CN101117357A (en) * | 2007-09-17 | 2008-02-06 | 中国农业大学 | Method for preparing water-soluble beta-1,3/1,6-dextran |
Non-Patent Citations (1)
| Title |
|---|
| 夏枚生等: "电气石对液态水团簇和沼泽红假单胞菌脱氢酶活性的影响", 《硅酸盐学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113121719A (en) * | 2021-06-03 | 2021-07-16 | 南京先达医药科技有限公司 | Extraction process of yeast-beta-glucan |
| CN113121719B (en) * | 2021-06-03 | 2022-03-04 | 南京先达医药科技有限公司 | Extraction process of yeast-beta-glucan |
| CN114163548A (en) * | 2021-12-13 | 2022-03-11 | 常州市第二人民医院 | Production process of yeast glucan |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109797179B (en) | 2021-02-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jeihanipour et al. | Ethanol production from cotton-based waste textiles | |
| CN101148830B (en) | Method for separating straw components by carrying steam explosion-microwave coupling treatment to plant straw | |
| CN102153763B (en) | Lignocellulose acid/alkali coupling pretreatment method | |
| CN109797179A (en) | A kind of environment-friendly preparation method thereof of yeast dextran | |
| CN101629191B (en) | Ultrasound-assisted enzymolysis wheat bran method for preparing ferulic acid | |
| CN102532329B (en) | Preparation method of low-viscosity octenyl succinic anhydride modified starch | |
| CN104211827B (en) | A kind of method extracting polysaccharide from Hericium erinaceus (Bull. Ex Fr.) Pers. dreg | |
| CN106491662B (en) | A method of polysaccharide and flavones are extracted simultaneously from Phellinus cultivation waste material | |
| CN101787381A (en) | Method for preparing fermentable reducing sugar by adopting ionic liquids to treat cellulose biomass | |
| CN101338333A (en) | Method for conversing agricultural and forest residues to be fermentable sugars | |
| CN104805151A (en) | Lignocellulosic feedstock pretreatment method without prepreg | |
| WO2010034220A1 (en) | Method for producing ethanol from raw material containing cassava residues | |
| CN101857639B (en) | Method for preparing sodium carboxymethylcellulose from residues generated by producing biobutanol from maize straw | |
| CN104894174A (en) | Method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation | |
| CN103243138A (en) | Method for preparing corncob xylooligosaccharide by a complex enzyme method | |
| CN102603917A (en) | Method for extracting yeast glucosan by utilizing enzymatic method | |
| CN103478410B (en) | Probiotic protein feed produced through peltate yam rhizomes and production method thereof | |
| CN108048495A (en) | A kind of biological extraction process of resveratrol | |
| CN103907938A (en) | Preparation method of jujube fruit residue dietary fiber | |
| CN103194504B (en) | Application of sulfonated lignin in wood fibre material enzyme hydrolysis saccharification | |
| WO2018133619A1 (en) | Method for producing sugar by hydrolyzing thick mash of agricultural and forest biomass raw material | |
| CN105803017A (en) | Method for improving wood fiber material enzyme hydrolysis saccharification efficiency | |
| CN102807629A (en) | Method for extracting rice bran polysaccharides by using continuous countercurrent ultrasonic equipment | |
| CN101333552B (en) | Process for producing yam saponin and co-producing glucose by acid-enzyme method prehydrolyzing turmeric | |
| CN109535443A (en) | The method for hydrolysis of lignocellulosic |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |