WO2018133619A1

WO2018133619A1 - Method for producing sugar by hydrolyzing thick mash of agricultural and forest biomass raw material

Info

Publication number: WO2018133619A1
Application number: PCT/CN2017/117785
Authority: WO
Inventors: 孙付保; 洪嘉鹏; 唐松; 周豪
Original assignee: 江南大学
Priority date: 2017-01-18
Filing date: 2017-12-21
Publication date: 2018-07-26
Also published as: CN106755198B; CN106755198A

Abstract

Provided is a method for producing sugar by hydrolyzing a thick mash of an agricultural and forest biomass raw material, comprising the following steps: (1) subjecting a cellulosic raw material to a digestion treatment in a glycerol phase, wherein a glycerol crude cellulose and a glycerol treatment liquid are obtained after separation; (2) recycling the above-mentioned collected glycerol treatment liquid in the digestion treatment in step (1) 7 to 11 times; and (3) hydrolyzing, by means of cellulase, the glycerol crude cellulose obtained in step (2) to obtain a fermentable sugar. The glycerol phase digestion treatment refers to a digestion temperature of 230ºC-270ºC at a gauge pressure of 0-5 atm and maintaining the temperature for 10-30 minutes; or the acid addition amount of 0.01%-0.3% (w/v) solution volume, a digestion temperature of 160ºC-240ºC at a gauge pressure of 0-5 atm and maintaining the temperature for 10-30 minutes; or the alkali addition amount of 0.01%-0.3% (w/v) solution volume, a digestion temperature of 160ºC-240ºC at a gauge pressure of 0-5 atm and maintaining the temperature for 10-30 minutes.

Description

Method for concentrating hydrolysis and producing sugar of agricultural and forestry biomass raw materials

Technical field

The invention relates to the field of resource utilization of agricultural and forestry biomass materials and the technical field of biomass chemicals, in particular to a method for saccharifying and saccharifying a fibrous raw material.

Background technique

With the increasingly prominent problems of resource and energy crisis, greenhouse effect and environmental pollution, the use of abundant agricultural and forestry cellulose-based biomass renewable resources, bio-refining can replace petrochemical production of bio-liquid fuels and bio-based chemicals. The current mainstream of the world bioenergy industry. However, the real era of biomass economy has not yet been realized, and the biorefinery technology for bioenergy and bio-based products from agricultural and forestry fiber-based biomass still faces many bottlenecks, including lignocellulose-sugar platform (pretreatment, enzymatic hydrolysis). The pretreatment and enzymatic hydrolysis problems of sugar fermentation are particularly prominent.

At present, representative methods for fiber-based biomass pretreatment include: dilute sulfuric acid method, hot water method, steam explosion method, lime method, ionic liquid and low-boiling organic solvent, etc., which are used in pretreatment evaluation ( Economics, effectiveness, processing time, safety and environmental friendliness, as well as impact on subsequent processes, have problems to varying degrees: 1) most require high-pressure operation, there are safety hazards; 2) strong acid/base requires higher equipment materials 3) expensive reagents; 4) high energy consumption; 5) poor selectivity of components, residual large amount of lignin, resulting in unsatisfactory pretreatment effect; 6) large amount of fermentation inhibitors, affecting subsequent processes; 7) large amount of wastewater generated Easy to cause secondary pollution.

On the other hand, glycerin as a by-product of biodiesel (1/10 biodiesel), production has risen with the increasing global biodiesel, and there has been a serious oversupply, and these low-grade glycerin is refined into food and Pharmaceutical grade (~99.5%) requires advanced technology and high investment costs. Based on these problems, the present inventors have been working hard to establish a glycerol organic solvent pretreatment aspect of a fibrous raw material (CN101148830B; CN101440108B). At the same time, it has taken the lead in developing and establishing a pretreatment process for glycerol organic solvents, and has received the attention and attention of nearly 20 international research groups. The published English articles have been cited more than 50 times.

In terms of enzymatic hydrolysis, at present, although the production method of cellulosic ethanol (mainly including raw material pretreatment, enzymatic hydrolysis, ethanol fermentation, separation and purification, etc.) has been opened, too high enzymatic cost seriously hinders cellulosic ethanol. Commercialization process. Researchers believe that one of the main reasons for the high cost of enzymatic hydrolysis is that the cellulase saccharification does not reach the "three high" (high concentration, high conversion rate and high efficiency) index of starchy enzymatic hydrolysis. Therefore, researchers at home and abroad have studied fiber-rich saccharification in terms of exploring cost-effective pretreatment methods, improving the purity and enzymatic solubility of matrix cellulose, and reducing the production of fermentation inhibitors, in order to achieve the "three highs" of fermentable sugar. "index.

In summary, the present invention further studies and innovates the glycerol organic solvent pretreatment process, and investigates the concentrated enzymatic saccharification of glycerol pretreated cellulose, so that it has more industrial application potential.

Summary of the invention

The invention establishes an atmospheric pressure degreasing crude glycerin pretreatment method for realizing the separation of lignocellulosic biomass components in the early stage. The invention improves the cooking method and the washing mode in the pretreatment process, and has the following two advantages. : 1) Reduce cooking energy consumption, reduce washing water consumption, and avoid fermentation inhibitors; 2) Selective removal of lignin and glycerol cellulose can significantly improve enzymatic activity. In order to solve the problem that the current fibrous raw material has difficulty in enriching and enzymatic saccharification, the present invention provides a method for concentrating hydrolysis of sugar from agroforestry biomass raw materials, comprising the following steps:

(1) The fibrous raw material is pulverized to 3 to 50 mm.

(2) the fibrous raw material pulverized in the step (1) is cooked in glycerin, and after separation, glycerin crude cellulose and glycerin treatment liquid are obtained;

(3) The glycerin treatment liquid collected above is recycled for 7-11 times in the retort treatment described in the step (2).

(4) The glycerol crude cellulose obtained in the step (2) is hydrolyzed by a microorganism or a cellulase to obtain a sugar-based bioenergy or a chemical.

Further, the fibrous raw material includes one or more derived from agricultural and forestry biomass, industrial processing residues, kitchen waste, animal waste and algae, and any mixture thereof.

Further, in the step (2), glycerol refers to different concentrations of glycerin solution, usually including crude glycerin, industrial glycerin and refined glycerin.

Further, in the step (2), the mass ratio of the fibrous raw material to the glycerin (converted to pure glycerin) is 1:6-1:30.

Further, in the step (2), the glycerol autocatalytic cooking condition is: the cooking gauge pressure is 0-5 atm, the cooking temperature is 230-270 ° C, and the heat preservation is 1-30 min, and the method has the characteristics of normal pressure, high temperature and short time. , low requirements on the treatment device, and selective removal of some lignin and hemicellulose components in the fibrous raw material, resulting in autocatalytic glycerin crude cellulose, wherein the cellulose content is 55-65%, hemicellulose content It is 15-25% and the lignin content is 10-20%.

Further, in the step (2), the glyceric acid catalytic heat preservation cooking condition is: the cooking surface pressure is 0-5 atm, the acid addition amount is 0.01%-0.3% (w/v) solution volume, and the cooking temperature is 160-240 ° C, Insulation for 1-30min, heat preservation for 1-30min, the method has the characteristics of normal pressure, high temperature and short-time, low requirements on the treatment device, and the selective removal of most hemicellulose components in the fibrous raw material, resulting in acid catalysis Glycerol crude cellulose having a cellulose content of 55-70%, a hemicellulose content of 2-10%, and a lignin content of 20-30%.

Further, in the step (2), the glycerol-base catalyzed heat-cooking condition is: the cooking table pressure is 0-5 atm, the alkali amount is 0.01%-0.3% (w/v) solution volume, and the cooking temperature is 160-240 ° C. Insulation for 1-30min, the method has the characteristics of atmospheric pressure and short-time, low requirements on the treatment device, and the selective removal of most lignin in the fiber, and the production of alkali-catalyzed glycerin crude cellulose, wherein the cellulose content is 55. -60%, hemicellulose content is 25-30%, and lignin content is 5-10%.

Further, in the step (2), the separation is directly washed with tap water 1-3 times, so that the glycerin content of the glycerin cellulose matrix does not exceed 15-20%.

Further, the glycerin treatment liquid includes a fermentation inhibitor, the fermentation inhibitor is furfural and 5-hydroxymethylfurfural, and the total amount of furfural and 5-hydroxymethylfurfural is 0.1-1.0 Torr (w/w).

Further, during the glycerol cooking process, the glycerol molecule is linked to the monosaccharide (glucose and xylose) or the oligosaccharide (cellobiose, xylobiose, etc.) by a glycosidic bond in the presence of a high temperature or a base. a coupling reaction occurs, and in the step (2), the glycerin pretreatment liquid contains glycerol glycoside, glycerol glucoside, and glycerol oligoglucoside, which are glycerol oligoglycoside, glycerol xyloside, and glycerol xylan glycoside. One or several. These products have moisturizing and anti-oxidation functions and can be used as cosmetic additives, and are by-products having high added value in the present invention.

Further, in the step (4), the crude glycerin fiber concentration is 20-40%, the cellulase addition amount is 2-15 FPU·g ^-1 glycerol crude cellulose, and the hydrolysis rate is 72-96 h, and the cellulolytic rate can be reached. 70–90%.

Advantageous effects of the invention

(1) The matrix of the invention has strong applicability and can be widely applied to different kinds of agricultural and forestry biomass raw materials;

(2) The pretreatment of the invention belongs to the selective dissolution type of lignin, and the lignin content in the solid matrix after pretreatment can be less than 10%, the purity of the cellulose can reach 60-70%, and the high concentration hydrolysis and concentration of the cellulose raw material can be achieved. Fermentation, the cellulose raw material treated by the method of the invention is subjected to enzymatic hydrolysis, the enzymatic hydrolysis rate can reach 75% or more, and the reducing sugar concentration can reach 171 g·L ^-1 ;

(3) In the alkali-added pre-catalyzed treatment method of the present invention, the amount of alkali added is small, and only 0.01%-0.3% (w/v) of the solution volume is required, which is advantageous for the continued use of the subsequent glycerin treatment liquid, and on the other hand. Effectively reduce the energy consumption of sewage treatment;

(4) The glycerol lignin of the present invention has the characteristics of high purity, small molecular weight, narrow distribution, and abundant active groups, and can be used for further fermentation; and compared with other pretreatment solvents (organic solvent or ionic liquid), glycerin is A variety of microbes have excellent carbon sources, and a small amount of residues have no significant adverse effects on subsequent microbial/enzymatic activities;

(5) The pretreatment method of the present invention hardly produces fermentation inhibitors (furfural and hydroxymethylfurfural, etc.), and the total amount of furfural and 5-hydroxymethylfurfural after treatment is 0.1-1.0 ‰ (w/w);

(6) The pretreatment of the present invention uses the biodiesel industrial by-product glycerin for the pretreatment of the cellulosic ethanol industry, and contributes to the simultaneous industrialization of biodiesel and bioethanol;

(7) The invention has the characteristics of constant pressure and short time to reduce energy consumption regardless of whether it is self-catalyzed or alkali-catalyzed pretreatment, and the alkali-catalyzed pretreatment can reduce the pretreatment temperature to further consume energy;

(8) Moreover, the present invention is simple in washing mode, avoids the hot glycerin-water solution washing and hot water washing mentioned in the published patent, and washes by controlling the residual amount of glycerin, usually washing with tap water 1-2 The glycerin pretreatment liquid can be recycled in the invention.

The above description is only an overview of the technical solutions of the present invention, and the technical means of the present invention can be more clearly understood and can be implemented in accordance with the contents of the specification. Hereinafter, the preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

DRAWINGS

Figure 1. Example 3 post-pretreatment matrix batch concentration enzyme.

detailed description

The specific embodiments of the present invention are further described in detail below with reference to the drawings and embodiments. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

Example 1:

The collected air-dried bagasse was sieved to 5-20 mm.

Then, 100 g of the above bagasse and 1.4 kg of industrial glycerin were placed in a 5-liter three-necked flask, heated at a stirring rate of 250 rpm to 240 ° C, and then incubated for 10 minutes. After completion of the reaction, the mixture was cooled to 100 ° C, washed with 1.5 kg of boiling water, mechanically stirred, and further cooled to 50-60 ° C. Because glycerin is denser and has a lower viscosity, some of the removed lignin is often adhered to the surface of the material and is not completely dissolved in glycerin. Therefore, adding boiling water to form an aqueous glycerin solution and mechanically stirring it is more conducive to the dissolution of lignin. Then, it was filtered with a G4 core funnel, and then filtered with tap water for 2 times (2 kg each time); the filter residue was glycerin crude cellulose, the mass of which was 62% of bagasse, and its cellulose content was 61%, hemicellulose. The content is 26%, the lignin content is 13%, and the glycerin residue is 0.39% of the dry weight of the matrix. The removal rates of hemicellulose and lignin are 40% and 65%, respectively. The filtrate is a glycerin treatment solution containing obvious glycerol glycosides (glycerol glucoside and glycerol oligoglucoside, glycerol glycoside is glycerol oligoglycoside, glycerol xyloside and glycerol xylan glycoside), two main fermentation inhibitors 5-hydroxyl Methylfurfural and furfural were 0.66 g/kg bagasse and 0.20 g/kg bagasse, respectively.

Take 5 g of the above glycerin crude cellulose, and add 25 mL of citric acid buffer (pH 4.8). At this time, the substrate concentration is 20% (w/v), and the cellulase C-Tce2 (141FPU·g ^-1 ) is added in an amount of 6 FPU· g ^-1 matrix, 180 rpm, enzymatic hydrolysis at 50 °C for 48 h, the enzymatic hydrolysis rate reached 72%, the reducing sugar concentration was 125.06 g·L ^-1 , the enzymatic hydrolysis rate reached 80% at 72 h, and the reducing sugar concentration at this time was 138.9 g·L. ^-1 .

Example 2:

The collected bagasse was air-dried and sieved to collect materials in the range of 5-20 mm.

Then, 100 g of the above-mentioned pulverized bagasse and 1.0 kg of the above industrial glycerin were placed in a 5-liter three-necked flask cooking apparatus, and 1 ml of concentrated sulfuric acid (the amount of sulfuric acid added was 0.1%, w/v) was heated and heated, and stirred at 250 rpm, and the temperature was raised to Heat at 230 ° C for 30 minutes. After the reaction is completed, the mixture is cooled to 100 ° C, 1.5 kg of boiling water is added, mechanically stirred, and the temperature is lowered to 50-60 ° C, and filtered with a G4 sand core funnel, and the filter residue is further filtered with 2 kg of tap water, and filtered by a G4 sand core funnel; The filter residue is glycerin crude cellulose, the quality of which is 57.9% of bagasse before pretreatment, the cellulose content is 64%, the hemicellulose content is 6.61%, the lignin content is 18.14%, and the glycerin residue is 22.3% dry weight. The cellulose and lignin removal rates were 83.9% and 49.4%, respectively. The filtrate is a glycerin treatment solution containing obvious glycerol glycosides (glycerol glucoside and glycerol oligoglucoside, glycerol glycoside is glycerol oligoglycoside, glycerol xyloside and glycerol xylan glycoside), and no fermentation inhibition is detected in the glycerol treatment solution. Furfural, but 5-hydroxymethylfurfural content is also very low, only 0.09g / kg bagasse.

Take 5 g of the above glycerin crude cellulose, and add 25 mL of citric acid buffer (pH 4.8). At this time, the substrate concentration is 20% (w/v), and the cellulase C-Tce2 (141FPU·g ^-1 ) is added in an amount of 6 FPU· g ^-1 matrix, 180 rpm, enzymatic hydrolysis at 50 °C for 48 h, the enzymatic hydrolysis rate reached 75%, the reducing sugar concentration was 117.4 g·L ^-1 , the enzymatic hydrolysis rate reached 83% at 72 h, and the reducing sugar concentration at this time was 131.2 g·L. ^-1 .

Example 3:

The collected air-dried bagasse was sieved to 5-20 mm.

Then, 100 g of the above-mentioned pulverized bagasse and 1.4 kg of the above industrial glycerin solution were placed in a cooking device, and 3 g of sodium hydroxide (0.2% sodium hydroxide, w/w) was added thereto, and the mixture was heated and stirred at 200 rpm, and cooked at 240 ° C. minute. After the reaction is completed, it is cooled to 100 ° C, 1.5 kg of boiling water is added, mechanically stirred, and the temperature is lowered to 50-60 ° C. The G4 sand core funnel is suction filtered, the filter residue is eluted with normal temperature tap water, and the G4 sand core funnel is filtered twice (2 kg each time). The filter residue is glycerin crude cellulose, the mass of which is 64% of the bagasse before the reaction, the cellulose content is 58%, the hemicellulose content is 28%, the lignin content is 10%, and the glycerin residue is 0.47% of the substrate dry weight. Correspondingly, the removal rates of hemicellulose and lignin were 20% and 70%, respectively. The filtrate is a glycerin treatment solution containing obvious glycerol glycosides (glycerol glucoside and glycerol oligoglucoside, glycerol glycoside is glycerol oligoglycoside, glycerol xyloside and glycerol xylan glycoside); two main fermentation inhibitors 5-hydroxyl Methylfurfural and furfural were 0.57 g/kg bagasse and 0.21 g/kg bagasse, respectively.

Take the above glycerol crude cellulose 3.75g (15% matrix concentration, w/v) as the initial concentration of enzymatic hydrolysis, and add cellulase C-Tce2 (141FPU·g ^-1 ) at a concentration of 35% matrix. 6FPU·g ^-1 matrix, a certain amount of citrate buffer (pH 4.8) was added, and the whole enzymatic hydrolysis system was added in an amount of 25 mL, and enzymatic hydrolysis was carried out at 180 rpm and 50 °C. 1.25 g of substrate (5%, w/v) were added at four time points of 12 hr, 18 hr, 24 hr and 30 hr after the start of enzymatic hydrolysis, so that the final substrate concentration was 35% (w/v). When the enzyme was hydrolyzed for 72 hr, the glucose concentration reached 171 g·L ^-1 , and the cellulose conversion rate at this time was 75.5%.

Example 4:

Then, 100 g of the above-mentioned pulverized bagasse and 1.0 kg of the above industrial glycerin were placed in a 5-liter three-necked flask cooking apparatus, and 1 mL of concentrated sulfuric acid (the amount of sulfuric acid added was 0.1%, w/v) was heated and heated, and stirred at 250 rpm. Heat up to 220 ° C for 30 minutes. After the reaction, the mixture was cooled to 100 ° C, 1.5 kg of boiling water was added, mechanically stirred, and the temperature was lowered to 50-60 ° C. The mixture was filtered with a G4 sand core funnel, and the filter residue was filtered with tap water and filtered with a G4 core funnel twice. 2kg); the filter residue is glycerin crude cellulose, the quality of which is 58.9% of bagasse before pretreatment, the cellulose content is 57.37%, the hemicellulose content is 7.76%, the lignin content is 24.27%, and the glycerol residue is the substrate dry weight. At 0.31%, the removal rates of hemicellulose and lignin were 80.79% and 31.22%, respectively. The filtrate is a glycerin treatment solution containing obvious glycerol glycosides (glycerol glucoside and glycerol oligoglucoside, glycerol glycoside is glycerol oligoglycoside, glycerol xyloside and glycerol xylan glycoside), and no fermentation inhibition is detected in the glycerol treatment solution. 5-hydroxymethylfurfural, but the furfural content is also very low, only 0.09g / kg of bagasse.

Take the above glycerin crude cellulose 3.75g (15% matrix concentration, w/v) as the initial concentration of the enzymatic hydrolysis, and add the cellulase C-Tce2 (141FPU·g ^-1 ) at a 30% matrix concentration. 10FPU·g ^-1 matrix, a certain amount of citrate buffer (pH 4.8) was added, and the whole enzymatic hydrolysis system was added in an amount of 25 mL, and enzymatic hydrolysis was carried out at 180 rpm and 50 °C. A 3.75 g matrix (15%, w/v) was added in one portion at 12 hr after the start of enzymatic hydrolysis so that the final substrate concentration was 30% (w/v). The content of reducing sugar reached 163.2 g/L when enzymatically hydrolyzed at 72 hr, and the cellulose conversion rate at this time was 83%.

Example 5:

The collected air-dried bagasse was sieved to 5-20 mm.

Then, 100 g of the above-mentioned pulverized bagasse and 1.4 kg of the above industrial glycerin solution were placed in a cooking apparatus, and 3 g of sodium hydroxide (0.2% sodium hydroxide, w/w) was added thereto, and the mixture was heated and stirred at 200 rpm, and cooked at 230 ° C. minute. After the reaction is completed, it is cooled to 100 ° C, 1.5 kg of boiling water is added, mechanically stirred, and the temperature is lowered to 50-60 ° C. The G4 sand core funnel is suction filtered, the filter residue is eluted with normal temperature tap water, and the G4 sand core funnel is filtered twice (2 kg each time). The filter residue is glycerin crude cellulose, the mass of which is 57% of the bagasse before the reaction, the cellulose content is 55%, the hemicellulose content is 28%, the lignin content is 12%, and the glycerin residue is 0.36% of the substrate dry weight. Correspondingly, the removal rates of hemicellulose and lignin were 12% and 60%, respectively. The filtrate is a glycerin treatment solution containing obvious glycerol glycosides (glycerol glucoside and glycerol oligoglucoside, glycerol glycoside is glycerol oligoglycoside, glycerol xyloside and glycerol xylan glycoside); two main fermentation inhibitors 5-hydroxyl Methylfurfural and furfural were 0.56 g/kg bagasse and 0.16 g/kg bagasse, respectively.

Take the above glycerol crude cellulose 3.75g (15% matrix concentration, w/v) as the initial concentration of enzymatic hydrolysis, and add cellulase C-Tce2 (141FPU·g ^-1 ) at a concentration of 35% matrix. 6FPU·g ^-1 matrix, a certain amount of citrate buffer (pH 4.8) was added, and the whole enzymatic hydrolysis system was added in an amount of 25 mL, and enzymatic hydrolysis was carried out at 180 rpm and 50 °C. 1.25 g of substrate (5%, w/v) were added at four time points of 12 hr, 18 hr, 24 hr and 30 hr after the start of enzymatic hydrolysis, so that the final substrate concentration was 35% (w/v). The content of reducing sugar reached 215 g/L at 72 hr, and the cellulose conversion rate at this time was 79.8%.

Although the present invention has been disclosed in the above preferred embodiments, the present invention is not limited thereto, and various modifications and changes can be made thereto without departing from the spirit and scope of the invention. The scope of the invention should be determined by the scope of the claims.

Claims

A method for concentrating hydrolysis of sugar from agroforestry biomass raw material, characterized in that it comprises the following steps:

(1) The fibrous raw material is digested in the glycerin phase, and the glycerin crude cellulose and glycerin treatment liquid are obtained after separation; wherein the glycerin phase cooking treatment refers to a cooking temperature of 230-270 ° C at a gauge pressure of 0-5 atm, and the temperature is maintained for 10-30 min. Or add acid volume of 0.01%-0.3% (w / v) solution volume, gauge pressure 0-5atm cooking temperature 160-240 ° C, heat preservation 10-30min; or alkali addition amount of 0.01%-0.3% (w / v) solution volume, gauge pressure 0-5atm cooking temperature 160-240 ° C, holding 10-30min;

(2) recycling the collected glycerin treatment liquid in the cooking process described in step (1) 7-11 times;

(3) The glycerin crude cellulose obtained in the step (2) is hydrolyzed by a cellulase to obtain a fermentable sugar.
A method for concentrating hydrolysis of sugar from agroforestry biomass raw material, characterized in that it comprises the following steps:

(1) the fibrous raw material is digested in a glycerin phase, and after separation, a glycerin crude cellulose and a glycerin treatment liquid are obtained;

(2) recycling the collected glycerin treatment liquid in the cooking process described in step (1) 7-11 times;

(3) The glycerin crude cellulose obtained in the step (2) is hydrolyzed by a cellulase to obtain a fermentable sugar.
The method for enriching hydrolyzed sugar production of agricultural and forestry biomass raw materials according to claim 1, wherein the step (1) comprises a glycerin phase characterized by: glycerin content of 95-100% anhydrous glycerol, mainly involving industrial glycerin and refined glycerin, Refers to the glycerin phase of 30%-80% crude glycerol solution concentrated by heating.
According to the step (1) of claim 1, the glycerin phase cooking treatment is mentioned, characterized in that the fibrous raw material is subjected to retort pretreatment in the glycerin phase without adding any catalyst, and the pretreatment conditions are: cooking temperature at a gauge pressure of 0-5 atm. It is 230-270 ° C, and the temperature is kept for 1-30 min. The method has the characteristics of short pressure at normal pressure, low requirements on the treatment device, and selective removal of some lignin and hemicellulose, resulting in autocatalytic glycerin crude cellulose.
The glycerin phase cooking treatment according to the step (1) of claim 1 is characterized in that the acid addition amount is from 0.01% to 0.3% (w/v) of the solution volume, and the gauge pressure is from 0 to 5 atm, and the cooking temperature is from 160 to 240 ° C. Insulation for 1-30 min, the method has the characteristics of short-time at normal pressure, realizes selective removal of most hemicellulose components, and produces acid-catalyzed glycerin crude cellulose.
According to the step (1) of claim 1, the glycerin phase cooking treatment is mentioned, characterized in that the alkali addition amount is 0.01%-0.3% (w/v) of the solution volume, the gauge pressure is 0-5 atm, the cooking temperature is 160-240 ° C, and the heat preservation is performed. 1-30min, the method has the characteristics of short-time at normal pressure, realizes the selective removal of most lignin, and produces alkali-catalyzed glycerin crude cellulose.
The method for concentrating hydrolyzed sugar production of agricultural and forestry biomass raw materials according to claim 1, wherein in the step (1), the separating means directly washing with tap water during filtration or centrifugation to control glycerin residues in the glycerol cellulose matrix. Not more than 15-20%.
The autocatalytic glycerin crude cellulose according to claim 3, which has a cellulose content of 55 to 65%, a hemicellulose content of 15 to 25%, and a lignin content of 15 to 20%.
The acid-catalyzed glycerol crude cellulose according to claim 4, which has a cellulose content of 55 to 70%, a hemicellulose content of 5 to 10%, and a lignin content of 20 to 30%.
The base-catalyzed glycerin crude cellulose according to claim 5, which has a cellulose content of 55 to 60%, a hemicellulose content of 25 to 30%, and a lignin content of 5 to 10%.
The method for concentrating hydrolyzed sugar production of agroforestry biomass raw material according to claim 1, wherein in the step (3), the glycerin crude cellulose has a substrate concentration of 10-40% and a cellulase addition amount of 2 When the -15FPU·g -1 glycerol crude cellulose is 72h, the cellulolytic rate can reach 70-90%.