CN101338333A - Method for conversing agricultural and forest residues to be fermentable sugars - Google Patents
Method for conversing agricultural and forest residues to be fermentable sugars Download PDFInfo
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- CN101338333A CN101338333A CNA2008100215313A CN200810021531A CN101338333A CN 101338333 A CN101338333 A CN 101338333A CN A2008100215313 A CNA2008100215313 A CN A2008100215313A CN 200810021531 A CN200810021531 A CN 200810021531A CN 101338333 A CN101338333 A CN 101338333A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Abstract
The invention belongs to the field of cellulase hydrolysis and discloses a method for converting forestry and agricultural residues into fermentability sugar. The method includes adopting steam explosion pre-treatment and washing on the forestry and agricultural residues after being cracked; then zymohydrolysis and saccharification is carried out. An activator is used during the zymohydrolysis and saccharification process. Compared with the prior method, as the activator is added to stop the lignin to generate non-productability absorption to the cellulose, the method of the invention improves the effective concentration of the cellulose and solves the problem of more consumption of the cellulose during the traditional hydrolyzing technique. The method of the invention has the characteristics of low cellulose consumption, simple technique, high sugar conversion rate, etc.
Description
Technical field
The invention belongs to the cellulase hydrolysis field, relate to a kind of method that agriculture and forestry organic waste material is converted into fermentable sugars that cellulose conversion gets sugared rate that improves.
Technical background
Along with the fast development and the progress of science and technology of industry, the exhaustion day by day of Nonrenewable resources and the progressively raising that environmental protection standard is required press for the exploitation substitutability energy new, cleaning.The agriculture and forestry organic waste material raw material is the very huge renewable resources of occurring in nature quantity, also is simultaneously to produce the huge potential resources of alcoholic acid, to solving energy problem, very large potentiality is arranged.China is large agricultural country, profuse agricultural crop straw raw material is arranged, annual about 700,000,000 tons of the agricultural crop straw that produces, comprising 2.3 hundred million tons of rice straws, 2.2 hundred million tons of maize straws, 1.2 hundred million tons of wheat stalks, 100,000,000 tons of beans and coarse cereals crop materials, other has 100,000,000 tons of stalks such as peanut, but utilization ratio is very low at present, less than 20%.Overwhelming majority agricultural crop straw is directly burned, and has both caused serious environmental to pollute, and has also produced the surprising wasting of resources simultaneously.
The saccharification technology of agriculture and forestry organic waste material raw material is a biomass material key for high-efficient use technology.The agriculture and forestry organic waste material raw material mainly contains Mierocrystalline cellulose, hemicellulose and xylogen.Three kinds of components are organic combines closely for this.Hemicellulose and xylogen are built by covalency and are formed tight network structure, form micro-fibril by hydrogen bond between the cellulosic molecule, crystallizing field and pars amorpha, crystalline texture makes cellulose polymer compound demonstrate rigidity and height water-insoluble, and destroying these structures has important effect for the saccharification of agriculture and forestry organic waste material.
The saccharification of cellulose technology mainly contains acid hydrolysis and two kinds of methods of enzymic hydrolysis.Acid-hydrolysis method occurs very early, the reaction needed High Temperature High Pressure, the energy consumption height, requirement for anticorrosion to equipment is also high, environmental issue is serious, and not only the sugar fermentation yield is low for hydrolytic process, and the sugar that produces further is degraded to by product furfural and furfural acid easily, follow-up ethanol fermentation there is very big restraining effect, the trend that is replaced by enzyme hydrolysis method is gradually arranged at present.The topmost advantage of enzymic hydrolysis is that equipment requirements is low, and energy consumption is little, the reaction conditions gentleness, and product is more single, helps the fermentation of follow-up sugar.The subject matter that faces is the influence that enzymolysis efficiency often is subjected to the agriculture and forestry organic waste material fibrous texture, and the hydrolysis cycle is longer, and the cost that enzyme utilizes is higher, and becoming is the bottleneck of enzymic hydrolysis industrialization maximum.
The major reason that the enzymolysis yield is low is to contain xylogen in the natural fiber raw material, agriculture and forestry organic waste material is difficult to removal xylogen in full force and effect pretreated the time, and a part of cellulase causes cellulase enzymolysis yield in enzymolysis process not high owing to being caused the cellulase waste by the nonproductive xylogen surface that is adsorbed in the enzymolysis process.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can improve enzymolysis yield, the cost method that agriculture and forestry organic waste material is converted into fermentable sugars low, easy and simple to handle.
The objective of the invention is to realize by following technical measures:
A kind of agriculture and forestry organic waste material is converted into the method for fermentable sugars, this method comprises the following steps:
A. the agriculture and forestry organic waste material after the agriculture and forestry organic waste material raw material being pulverized with physical method adopts the steam explosion treatment, and vapor pressure is to keep 4～15min under 1.0～1.8MPa condition;
C. be 5～10 times water washing of its dry weight with the agriculture and forestry organic waste material weight after the steam explosion, wash 3～5 times,, filter and obtain the steam explosion slag,, dry standby as hydrolysis material to neutral;
D. steam explosion slag and promoting agent are inserted in the hydrolysis reactor, add entry, pH damping fluid or acid, alkali miscible to the solid-liquid weight ratio be 1: 5～1: 20, pH is 3.5～6.0 in control, the cellulase consumption of every gram over dry steam explosion slag is controlled at 4～40FPIU/g, under 30～70 ℃ of conditions, carry out enzymolysis, agitation condition: 50～300r/min, the reaction times is 10～48 hours.
Described method, wherein agriculture and forestry organic waste material is one or more in maize straw, straw, grass stalk, kaoliang stalk, reed, fiber crops, flax, hemp, the corn cob.
Described method, wherein acid is hydrochloric acid or sulfuric acid; Alkali is sodium hydroxide or potassium hydroxide; Transferring the pH damping fluid is phosphoric acid/sodium phosphate buffer, acetic acid/sodium-acetate buffer, citric acid/sodium citrate damping fluid.
Described method, wherein promoting agent is polyoxyethylene glycol (PEG), polyamino acid (PAA), alkyl-glucoside (APG) or sucrose fatty ester (SE).
Described method, wherein the promoting agent usage quantity is 1～10g/L enzymolysis solution.
Described method, wherein the miscible back of buffer system over dry steam explosion slag concentration is at 50～150g/L, and the cellulase consumption is 8～20FPIU/g over dry steam explosion slag.
Described method, wherein cellulase is to be provided by in Trichodermareesei (Trichoderma reesei), viride (Trichoderma viride), aspergillus niger (Aspergillus niger), the koning trichoderma (Trichoderma koningii) one or more.
Described method, wherein the molecular weight of polyoxyethylene glycol is 800～8000Da.
Described method, wherein polyamino acid is polyphenylalanine, poly aspartic acid or polytyrosine, and the molecular weight of above-mentioned three seed amino acids is respectively 1000～10000Da.
Described method, wherein the carbochain in the alkyl-glucoside is C
4～40Straight-chain paraffin, the fatty acid chain length in the sucrose fatty ester is C
4～30Straight-chain paraffin.
Beneficial effect of the present invention:
The present invention is directed to present cellulase not high problem of enzymolysis yield in enzymolysis process, utilize the technology of promoting agent, a kind of method that agriculture and forestry organic waste material is converted into fermentable sugars is provided the cellulase desorption.Substantive distinguishing features of the present invention and obtained technical progress are: by adding promoting agent in enzymatic hydrolysis system, make the xylogen in the substrate (agriculture and forestry organic waste material) weaken to the unproductive adsorption of cellulase, the effective concentration of cellulase in solution improves, reduce the loss that cellulase causes owing to unproductive absorption in enzymolysis process, thereby improved a kind of method of enzymolysis fermentable sugars yield.Advantage of the present invention also is embodied in:
1, cellulase utilization ratio height.Because the existence of promoting agent has reduced the unproductive absorption of cellulase, has improved the enzyme activity and the stability of cellulase.The present invention can improve fermentable sugars (glucose, down together) yield 10-15% after adding promoting agent.
2, shorten enzymolysis time and minimizing enzyme dosage, reduce the use cost of cellulase.Easy to operate, simple, be convenient to industrialized extensive use.
3, use agriculture and forestry organic waste material to make raw material, can effectively reduce production cost.
Embodiment
The invention will be further described below by embodiment.
The bacterium of the cellulase-producing that embodiment mentions and be the technology of well known to a person skilled in the art by the method for the fermentation using bacteria production of cellulose enzyme of cellulase-producing.
The fermentable sugars yield is that glucose yield accounts for that the Mierocrystalline cellulose theoretical conversion is the per-cent of glucose amount in the over dry steam explosion slag.
Embodiment 1: Trichodermareesei fermentative preparation cellulase.
1, enzyme nutrient solution prescription is produced in preparation: paper pulp 10g/L, and glucose 1.0g/L, peptone 1.0g/L, potassium primary phosphate 2.0g/L, magnesium sulfate heptahydrate 0.05g/L, calcium chloride 0.4g/L regulates pH to 5.0.
2, get the triangular flask that the 50mL nutrient solution places 250mL band tampon, QM9414 is inoculated in the triangular flask with Trichodermareesei (Trichodermareesei), cultivates on the constant temperature oscillator of 170r/min, produces the enzyme temperature and is controlled at (30 ± 1) ℃
3, cultivate after 4 days centrifugal 10min under 3000r/min, getting clear liquid is crude enzyme liquid, and recording filter paper enzyme activity is 2.15FPIU/mL.
Embodiment 2
1. crushed corn straws 22g is dropped in the steam explosion jar and handle 14min under the 1.2MPa condition, instant decompression discharges afterwards, obtain through the maize straw after the steam explosion processing, and use the clear water of 10 times of maize straw dry weights to wash 5 times it to neutral, air-dry standby.
With dried steam explosion slag with after pH4.8 citric acid/sodium citrate damping fluid mixes, add cellulase (available from Sigma company), the cellulase usage quantity is a 10FPIU/g maize straw over dry steam explosion slag, solid-to-liquid ratio is 1: 9 (weight ratio), adds promoting agent PEG80010g/L enzymolysis solution.
3. under 50 ℃ of conditions, agitation condition is: 100r/min, enzymolysis 48h.
4. the fermentable sugars yield that adopts above-mentioned condition to obtain is: 89%.
Comparative example 2:
1. crushed corn straws 22g is dropped in the steam explosion jar and handle 14min under the 1.2MPa condition, instant decompression discharges afterwards, obtain through the maize straw after the steam explosion processing, and use the clear water of 10 times of maize straw dry weights to wash 5 times it to neutral, air-dry standby.
With the steam explosion slag with after pH4.8 citric acid/sodium citrate damping fluid mixes, add cellulase (available from Sigma company), the cellulase usage quantity is a 10FPIU/g maize straw over dry steam explosion slag, solid-to-liquid ratio is 1: 9 (weight ratio).
3. under 50 ℃ of conditions, agitation condition is: 100r/min, enzymolysis 48h.
4. the fermentable sugars yield that adopts above-mentioned condition to obtain is: 74%.
Embodiment 3
1. the straw 24g after will pulverizing drops in the steam explosion jar and handles 7min under the 1.6MPa condition, instant decompression discharges afterwards, obtain the straw after the process steam explosion is handled, and use the clear water washing of 6 times of straw dry weights to wash extremely neutral, air-dry standby to clear water 4 times it.
With dried steam explosion slag with after water mixes, the sulfuric acid of use 1% and 1% NaOH regulate pH5.0, add cellulase (Trichodermareesei product), and the cellulase usage quantity is a 12FPIU/g straw over dry steam explosion slag, solid-to-liquid ratio is 1: 9, adds promoting agent PEG80001g/L enzymolysis solution.
3. under 40 ℃ of conditions, agitation condition is: 200r/min, enzymolysis 48h.
4. the fermentable sugars yield that adopts above-mentioned condition to obtain is: 78%.
Comparative Examples 2: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 2, and resulting fermentable sugars yield is: 67%.
Embodiment 3
1. handle 4min under the 1.8MPa condition in the straw stalk 20g input steam explosion jar after will pulverizing, instant decompression discharges afterwards, obtains through the straw stalk after the steam explosion processing, and uses the clear water of 5 times of straw stalk dry weights to wash 5 times to neutral it, and is air-dry standby.
With dried steam explosion slag with after pH4.6 acetic acid/sodium-acetate buffer mixes, add cellulase (the mould product of healthy and free from worry wood), the cellulase usage quantity is a 14FPIU/g straw stalk over dry steam explosion slag, and solid-to-liquid ratio is 1: 8, and cellulase adds promoting agent PEG40004g/L enzymolysis solution.
3. under 60 ℃ of conditions, agitation condition is: 50r/min, enzymolysis 36h.
4. the fermentable sugars yield that adopts above-mentioned condition to obtain is: 86%.
Comparative Examples 3: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 3, and resulting fermentable sugars yield is: 73%.
Embodiment 4
1. crushed corn straws 22g is dropped in the steam explosion jar and handle 14min under the 1.2MPa condition, instant decompression discharges afterwards, obtain through the maize straw after the steam explosion processing, and use the clear water of 7 times of maize straw dry weights to wash 3 times it to neutral, air-dry standby.
With dried steam explosion slag with after pH4.8 phosphoric acid/sodium phosphate buffer mixes, add cellulase (viride product), usage quantity is a 12FPIU/g maize straw over dry steam explosion slag, and solid-to-liquid ratio is 1: 7, adds promoting agent polyphenylalanine (molecular weight 5000Da) 4g/L enzymolysis solution.
3. under 45 ℃ of conditions, agitation condition is: 150r/min, enzymolysis 48h.
4. the fermentable sugars yield that adopts above-mentioned condition to obtain is: 88%.
Comparative Examples 4: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 4, and resulting fermentable sugars yield is: 74%.
Embodiment 5
1. handle 7min under the 1.2MPa condition in the straw 24g input steam explosion jar after will pulverizing, instant decompression discharges afterwards, obtains through the straw after the steam explosion processing, and uses the clear water of 8 times of straw dry weights to wash 4 times to neutral it, and is air-dry standby.
With dried steam explosion slag with after water mixes, use acid-alkali accommodation pH4.8, add cellulase (available from Sigma company), usage quantity is a 11FPIU/g straw over dry steam explosion slag, solid-to-liquid ratio is 1: 13, adds promoting agent poly aspartic acid (molecular weight 1000Da) 10g/L enzymolysis solution.
3. under 55 ℃ of conditions, agitation condition is: 170r/min, enzymolysis 48h.
4. the fermentable sugars yield that adopts above-mentioned condition to obtain is: 85%.
Comparative Examples 5: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 5, and resulting fermentable sugars yield is: 71%.
Embodiment 6
1. handle 4min under the 1.8MPa condition in the straw stalk 20g input steam explosion jar after will pulverizing, instant decompression discharges afterwards, obtains through the straw stalk after the steam explosion processing, and uses the clear water of 5 times of grass stalk dry weights to wash 5 times to neutral it, and is air-dry standby.
With dried steam explosion slag with after pH4.6 citric acid/sodium citrate damping fluid mixes, add cellulase (available from Sigma company), usage quantity is a 9FPIU/g straw stalk over dry steam explosion slag, solid-to-liquid ratio is 1: 8, adds promoting agent polytyrosine (molecular weight 10000Da) 2g/L enzymolysis solution.
3. under 60 ℃ of conditions, agitation condition is: 220r/min, enzymolysis 36h.
4. the fermentable sugars yield that obtains with above-mentioned condition is: 83%.
Comparative Examples 6: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 6, and resulting fermentable sugars yield is: 69%.
Embodiment 7
1. disintegrating Chinese sorghum stalk 25g is dropped in the steam explosion jar and handle 15Min under the 1.0MPa condition, instant decompression discharges afterwards, obtain through the sorghum stalk after the steam explosion processing, and use the clear water of 9 times of kaoliang stalk dry weights to wash 4 times it to neutral, air-dry standby.
With dried steam explosion slag with after pH4.8 acetic acid/sodium-acetate buffer mixes, add cellulase (available from Sigma company), usage quantity is a 8FPIU/g sorghum stalk over dry steam explosion slag, and solid-to-liquid ratio is 1: 15, adds promoting agent C-4 alkyl-glucoside 10g/L enzymolysis solution.
3. under 45 ℃ of conditions, agitation condition is: 120r/min, enzymolysis 36h.
4. the fermentable sugars yield that obtains with above-mentioned condition is: 87%.
Comparative Examples 7: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 7, and resulting fermentable sugars yield is: 72%.
Embodiment 8
1. the reed stalk 27g after will pulverizing drops in the steam explosion jar and handles 10min under the 1.2MPa condition, and instant decompression discharges afterwards, obtains through the reed stalk after the steam explosion processing, and uses the clear water washing to neutral it, and is air-dry standby.
With dried steam explosion slag with after pH4.8 phosphoric acid/sodium phosphate buffer mixes, add cellulase (available from Sigma company), solid-to-liquid ratio is 1: 12, and the cellulase usage quantity is a 8FPIU/g reed stalk over dry steam explosion slag, adds promoting agent C-40 alkyl-glucoside 1g/L enzymolysis solution.
3. under 40 ℃ of conditions, agitation condition is: 50r/min, enzymolysis 48h.
4. the fermentable sugars yield that obtains with above-mentioned condition is: 86%.
Comparative Examples 8: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 8, and resulting fermentable sugars yield is: 71%.
Embodiment 9
1. handle 7min under the 1.5MPa condition in the hemp stalk 35g input steam explosion jar after will pulverizing, instant decompression discharges afterwards, obtains through the hemp stalk after the steam explosion processing, and uses the clear water of 8 times of waste of flax dry weights to wash 4 times to neutral it, and is air-dry standby.
With dried steam explosion slag with after water mixes, use acid-alkali accommodation pH4.6, add cellulase (available from Sigma company), usage quantity is a 11FPIU/g hemp stalk over dry steam explosion slag, solid-to-liquid ratio is 1: 7, adds promoting agent C-25 alkyl-glucoside 6.0g/L enzymolysis solution.
3. under 55 ℃ of conditions, agitation condition is: 300r/min, enzymolysis 12h.
4. the fermentable sugars yield that obtains with above-mentioned condition is: 81%.
Comparative Examples 9: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 9, and resulting fermentable sugars yield is: 67%.
Embodiment 10
1. the hemp stalk 30g after will pulverizing drops in the steam explosion jar and handles 9min under the 1.6MPa condition, and instant decompression discharges afterwards, obtains through the hemp stalk after the steam explosion processing, and uses the clear water of 5 times of big waste of flax dry weights to wash 5 times to neutral it, and is air-dry standby.
With dried steam explosion slag with after pH4.6 citric acid/sodium citrate damping fluid mixes, add cellulase (available from Sigma company), usage quantity is a 8FPIU/g hemp stalk over dry steam explosion slag, and solid-to-liquid ratio is 1: 15, adds promoting agent C-4 sucrose fatty ester 10.0g/L enzymolysis solution.
3. under 60 ℃ of conditions, agitation condition is: 250r/min, enzymolysis 24h.
4. the fermentable sugars yield that obtains with above-mentioned condition is: 83%.
Comparative Examples 10: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 10, and resulting fermentable sugars yield is: 68%.
Embodiment 11
1. the corn cob 28g after will pulverizing drops in the steam explosion jar and handles 12min under the 1.5MPa condition, instant decompression discharges afterwards, obtain through the corn cob after the steam explosion processing, and use the clear water of 6 times of corn cob dry weights to wash 3 times it to neutral, air-dry standby.
With dried steam explosion slag with after pH4.8 phosphoric acid/sodium phosphate buffer mixes, add cellulase (available from Sigma company), usage quantity is a 9FPIU/g corn cob over dry steam explosion slag, and solid-to-liquid ratio is 1: 14, adds promoting agent C-30 sucrose fatty ester 1.0g/L enzymolysis solution.
3. under 50 ℃ of conditions, agitation condition is: 80r/min, enzymolysis 36h.
4. the fermentable sugars yield that obtains with above-mentioned condition is: 90%.
Comparative Examples 11: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 11, and resulting fermentable sugars yield is: 75%.
Embodiment 12
1. crushed corn straws 22g is dropped in the steam explosion jar and handle 14min under the 1.2MPa condition, instant decompression discharges afterwards, obtain through the maize straw after the steam explosion processing, and use the clear water of 8 times of maize straw dry weights to wash 4 times it to neutral, air-dry standby.
With dried steam explosion slag with after pH4.8 acetic acid/sodium-acetate buffer mixes, add cellulase (available from Sigma company), usage quantity is a 15FPIU/g maize straw over dry steam explosion slag, and solid-to-liquid ratio is 1: 15, adds promoting agent C-22 sucrose fatty ester 4.0g/L enzymolysis solution.
3. under 60 ℃ of conditions, agitation condition is: 270r/min, enzymolysis 48h.
4. the saccharification of cellulose rate that obtains with above-mentioned condition is: 88%.
Comparative Examples 12: except that not adding the promoting agent, all the other operation stepss and condition are with embodiment 12, and resulting fermentable sugars yield is: 73%.
Claims (10)
1. one kind is converted into the method for fermentable sugars with agriculture and forestry organic waste material, it is characterized in that this method comprises the following steps:
A. the agriculture and forestry organic waste material after the agriculture and forestry organic waste material raw material being pulverized with physical method adopts the steam explosion treatment, and vapor pressure is to keep 4～15min under 1.0～1.8MPa condition;
C. be 5～10 times water washing of its dry weight with the agriculture and forestry organic waste material weight after the steam explosion, wash 3～5 times,, filter and obtain the steam explosion slag,, dry standby as hydrolysis material to neutral;
D. steam explosion slag and promoting agent are inserted in the hydrolysis reactor, add entry, pH damping fluid or soda acid miscible to the solid-liquid weight ratio be 1: 5～1: 20, pH is 3.5～6.0 in control, the cellulase consumption of every gram over dry steam explosion slag is controlled at 4～40FPIU/g, under 30～70 ℃ of conditions, carry out enzymolysis, agitation condition: 50～300r/min, the reaction times is 10～48 hours.
2, method according to claim 1 is characterized in that described agriculture and forestry organic waste material is one or more in maize straw, straw, grass stalk, kaoliang stalk, reed, fiber crops, flax, hemp, the corn cob.
3, method according to claim 1 is characterized in that described acid is hydrochloric acid or sulfuric acid; Alkali is sodium hydroxide or potassium hydroxide; Transferring the pH damping fluid is phosphoric acid/sodium phosphate buffer, acetic acid/sodium-acetate buffer, citric acid/sodium citrate damping fluid.
4, method according to claim 1 is characterized in that described promoting agent is polyoxyethylene glycol, polyamino acid, alkyl-glucoside or sucrose fatty ester.
5, method according to claim 1 is characterized in that described promoting agent usage quantity is 1～10g/L enzymolysis solution.
6, method according to claim 1 is characterized in that the miscible back of buffer system over dry steam explosion slag concentration at 50～150g/L, and the cellulase consumption is 8～20FPIU/g over dry steam explosion slag.
7, method according to claim 1 is characterized in that cellulase is by one or more generations in Trichodermareesei (Trichodermareesei), viride (Trichoderma viride), aspergillus niger (Aspergillus niger), the koning trichoderma (Trichodermakoningii).
8, method according to claim 4, the molecular weight that it is characterized in that described polyoxyethylene glycol is 800～8000Da.
9, method according to claim 4, described polyamino acid are polyphenylalanine, poly aspartic acid or polytyrosine, and the molecular weight of above-mentioned three seed amino acids is respectively 1000～10000Da.
10, method according to claim 4 is characterized in that the carbochain in the alkyl-glucoside is C
4～40Straight-chain paraffin, the fatty acid chain length in the sucrose fatty ester is C
4～30Straight-chain paraffin.
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| ITRM20090290A1 (en) * | 2009-06-09 | 2010-12-10 | Enea Ente Nuove Tec | PLANT AND ITS PROCEDURE FOR THE PRE-TREATMENT OF LIGNOCELLULOSIC BIOMASS |
| CN103224966A (en) * | 2013-05-08 | 2013-07-31 | 天津大学 | Method for improving enzymatic hydrolysis saccharification efficiency of straws through dry storage coupling steam explosion pretreatment |
| CN103243136A (en) * | 2013-05-08 | 2013-08-14 | 天津大学 | Method for improving enzymolysis saccharification efficiency of lignocellulose |
| CN103243137A (en) * | 2013-05-08 | 2013-08-14 | 天津大学 | Method for improving straw enzymolysis saccharification efficiency by two-step crushing combined with steam explosion |
| CN103305490A (en) * | 2012-03-12 | 2013-09-18 | 六安市凯旋大麻纺织有限责任公司 | Method for producing pectinase in fermentation manner by taking waste hemp degumming liquid as carbon source |
| CN104136619A (en) * | 2011-12-21 | 2014-11-05 | 贝塔可再生能源公司 | An improved pre-hydrolysis step involving vacuum |
| CN104846032A (en) * | 2015-04-09 | 2015-08-19 | 镇江博睿兴邦生物科技有限公司 | Method for preparing cellulose hydrolyzed sugar by using cellulase |
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| CN107058426A (en) * | 2017-04-14 | 2017-08-18 | 中国科学技术大学 | A kind of preprocess method of agriculture and forestry organic waste material |
| CN107614694A (en) * | 2015-04-10 | 2018-01-19 | 彗星生物炼制公司 | For the product for handling the method and composition of cellulose series biomass and thus producing |
| CN107916284A (en) * | 2016-10-11 | 2018-04-17 | 中国科学院大连化学物理研究所 | Lignocellulosic using polyethylene glycol/aqueous mixtures as medium digests saccharification technology |
| CN115011651A (en) * | 2022-07-11 | 2022-09-06 | 雄安创新研究院 | Method for efficiently preparing sugar by using reed |
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| ITRM20090290A1 (en) * | 2009-06-09 | 2010-12-10 | Enea Ente Nuove Tec | PLANT AND ITS PROCEDURE FOR THE PRE-TREATMENT OF LIGNOCELLULOSIC BIOMASS |
| CN104136619A (en) * | 2011-12-21 | 2014-11-05 | 贝塔可再生能源公司 | An improved pre-hydrolysis step involving vacuum |
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