CN103243136A - Method for improving enzymolysis saccharification efficiency of lignocellulose - Google Patents
Method for improving enzymolysis saccharification efficiency of lignocellulose Download PDFInfo
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- CN103243136A CN103243136A CN2013101668148A CN201310166814A CN103243136A CN 103243136 A CN103243136 A CN 103243136A CN 2013101668148 A CN2013101668148 A CN 2013101668148A CN 201310166814 A CN201310166814 A CN 201310166814A CN 103243136 A CN103243136 A CN 103243136A
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Abstract
The invention discloses a method for improving the enzymolysis saccharification efficiency of lignocellulose, which comprises the following steps of: (1) crushing a lignocellulose raw material with a water mass of 30-75%, packaging the crushed material in a sealing device, and storing the crushed material; (2) adding the material obtained in the step (1) into a steam explosion reactor, heating the material by using steam, instantaneously releasing pressure so as to obtain a solid-liquid mixture, carrying out e solid-liquid separation on the solid-liquid mixture, carrying out washing and drying on the obtained solid; and (3) adding cellulase and water into the dried matter, adjusting the pH value of the obtained product to be 4.5-5.5, carrying out enzymolysis on the obtained product, and carrying out solid-liquid separation on the obtained product again so as to obtain a liquid rich in glucose and xylose. According to the method disclosed by the invention, the loss of biomass can be reduced, and the flexibility and porosity of a biomass structure can be maintained; the recovery rate of sugar is high, and degradation products are less; and the enzymolysis saccharification rate is significantly increased.
Description
Technical field
The present invention relates to the biomass technical field of new energies, particularly a kind of method that improves lignocellulose enzymatic saccharification efficient.
Background technology
Be badly in need of a kind of new forms of energy and substitute exhausted day by day fossil energy the world today, thereby reduce the caused economy of fossil energy, safety and environmental stress.Be that the bio-ethanol of raw material production is considered to the useful of fossil energy and replenishes with the lignocellulose, have advantages such as low-carbon environment-friendly, aboundresources, it is of value to realizes that national economy increases the purpose of energy security and environment protection.
The lignocellulose ethanol mainly comprises plantation and the unit operations such as harvesting, storage and pulverizing, pre-treatment, enzymolysis, fermentation and purification of raw material.Wherein the storage of raw material not only can influence biomass transhipment and physicochemical property, but also can follow-up pre-treatment, enzymolysis and the fermentation of remarkably influenced.Simultaneously, in cellulosic ethanol production, occupy very high specific gravity with the relevant required cost of raw material storage, and alcohol yied is had crucial effects.
Xylogen and hemicellulose make plain this body structure of natural wooden fiber tight to the crystalline structure of cellulosic provide protection and Mierocrystalline cellulose itself.If directly it is hydrolyzed, hydrolysis conversion is extremely low.Therefore, must carry out pre-treatment to Mierocrystalline cellulose.Pretreatment process mainly comprises physics method, chemical method, physico-chemical processes and biological process.Steam explosion is considered to a kind of preconditioning technique with broad prospect of application.The steam explosion process mainly comprises two processes: from hydrolytic process and blasting process.Under high pressure conditions, with steam pending raw material is heated to 160 ℃-260 ℃, keeps several minutes to tens minute, it is taken place fully from hydrolysis, then moment release of pressure, make the raw material explosion.Acetic acid and other acid catalysis hydrolysis of hemicellulose from hydrolytic process release.At high temperature, water is also as acid catalyzed reaction.From hydrolytic process, the softening and part degraded of the xylogen of middle lamella, thus weakened interfibrous adhesion effect.Blasting process, step-down rapidly make the gas phase media in the raw material spray, expand, externally do work, and cell is dissociated into single fibrocyte from middle lamella, thereby has increased raw material enzymolysis accessibility.The steam explosion pre-treatment has does not add any catalyzer, and the treatment time is short, advantages such as environmentally safe.
But the efficient utilization that realizes lignocellulose of how effectively the storage mode of lignocellulose being combined with the steam explosion pre-treatment does not still have research.
Summary of the invention
The objective of the invention is low at existing storage mode technical efficiency, cost is high, and the storing technology of poor efficiency can cause problems such as pre-treatment and enzymolysis efficiency reduction, and a kind of method that improves lignocellulose enzymatic saccharification efficient is provided.
Technical scheme concrete grammar step of the present invention is as follows:
A kind of method that improves lignocellulose enzymatic saccharification efficient comprises the steps:
(1) lignocellulosic material that will contain quality 30%-75% is pulverized, and is encapsulated in the tightness system storage;
(2) material that step (1) is obtained joins in the steam explosion reactor, uses steam heating, and abrupt release pressure obtains solidliquid mixture, and with the solidliquid mixture solid-liquid separation, solid washes with water, drying;
(3) dry thing adds cellulase and water, transfers pH=4.5-5.5, enzymolysis, and solid-liquid separation again obtains being rich in the liquid of glucose and wood sugar.
Step (1) is preferably: will contain the lignocellulosic material that quality is 30%-75%, it is long to be crushed to 0.5cm-4.0cm, is that 50g/L-150g/L is encapsulated in the tightness system with the density of lignocellulosic material dry weight, storage 1-12 month.
Step (2) is preferably: the material that step (1) is obtained joins in the steam explosion reactor, with being steam heated to 170 ℃-220 ℃, keeps 3-10min, abrupt release pressure, obtain solidliquid mixture, with the solidliquid mixture solid-liquid separation, solid washes with water, drying.
Step (3) is preferably: the ratio in the dry thing of 1g, 5FPU-36FPU cellulase and 9-24g water is added to the water dry thing, cellulase, transfers pH=4.5-5.5, enzymolysis 36-168h, and solid-liquid separation again obtains being rich in the liquid of glucose and wood sugar.
The quality that contains of lignocellulosic material is 35%-60%.
Lignocellulosic material is maize straw, broomcorn straw, wheat stalk or rice straw.
The length that lignocellulose is pulverized is 1.0cm-3.0cm.
The density that is encapsulated in the tightness system is 60g/L-120g/L.
The time of described storage is preferably 3-9 month.
Steam-heated temperature is preferably 190 ℃-210 ℃, keeps 5min-8min.
The present invention has following advantage:
(1) method of the present invention can reduce losing of biomass, keeps snappiness and the porousness of biomass structure simultaneously.
(2) Tang rate of recovery height, degraded product is few.The enzymatic saccharification rate significantly improves.
Embodiment
The present invention is further described below by specific embodiment:
Embodiment 1
A kind of method that improves lignocellulose enzymatic saccharification efficient comprises the steps:
(1) maize straw that will newly gather in is dried to that to contain quality be 60%, pulverizes with pulverizer then, and making length is 2.8 ± 0.2cm, is that 60g/L is encapsulated in the sealing bag with the density of lignocellulosic material dry weight, stores 9 months
(2) material that step (1) is obtained joins in the retort of steam explosion reactor, closes the material inlet valve, feeds steam heating, to temperature of reaction be 210 ℃, keep 5min, abrupt release pressure, in receiving tank, cooling 10min opens the material outlet valve with the material explosion, collect solidliquid mixture, solid-liquid separation, the solid water cleans, 45 ℃ of oven dry down, collect liquid and measure composition, the steam explosion pre-treatment dextran rate of recovery is 100%, and the xylan rate of recovery is 72%
(3) in the cellulase of the dried dry thing of 1g, 36FPU and the ratio of 18g water dry thing and cellulase are added in the water, with 1M sodium citrate buffer solution regulator solution pH=5.0, at 50 ℃, enzyme digestion reaction 168h in the 200rpm shaking table, solidliquid mixture is centrifugal 10min under 12000rpm, obtain being rich in the liquid of glucose and wood sugar, enzymolysis dextran transformation efficiency is 92%, and the xylan transformation efficiency is 87%.
Embodiment 2
A kind of method that improves lignocellulose enzymatic saccharification efficient comprises the steps:
(1) broomcorn straw that will newly gather in is dried to that to contain quality be 35%, pulverizes with pulverizer then, and making length is 3.8 ± 0.2cm, is that 50g/L is encapsulated in the sealed can with the density of lignocellulosic material dry weight, stores 3 months;
(2) material that step (1) is obtained joins in the retort of intermittent type steam explosion reactor, closes the material inlet valve, feeds steam heating, to temperature of reaction be 170 ℃, keep 10min, abrupt release pressure, in receiving tank, cooling 10min opens the material outlet valve with the material explosion, collect solidliquid mixture, solid-liquid separation, the solid water cleans, 45 ℃ of oven dry down, collect liquid and measure composition, the steam explosion pre-treatment dextran rate of recovery is 98%, and the xylan rate of recovery is 75%;
(3) in the cellulase of the dried dry thing of 1g, 5FPU and the ratio of 24g water dry thing and cellulase are added in the water, with 1M sodium citrate buffer solution regulator solution pH=5.5, at 50 ℃, enzyme digestion reaction 120h in the 200rpm shaking table, solidliquid mixture is centrifugal 10min under 12000rpm, obtain being rich in the liquid of glucose and wood sugar, enzymolysis dextran transformation efficiency is 87%, and the xylan transformation efficiency is 82%.
Embodiment 3
A kind of method that improves lignocellulose enzymatic saccharification efficient comprises the steps:
(1) wheat stalk that will newly gather in is dried to that to contain quality be 30%, pulverizes with pulverizer then, and making length is 1.2 ± 0.2cm, is that 120g/L is encapsulated in the sealing cellar for storing things with the density of lignocellulosic material dry weight, stores 1 month;
(2) material that step (1) is obtained joins in the retort of steam explosion reactor, closes the material inlet valve, feeds steam heating, to temperature of reaction be 190 ℃, keep 8min, abrupt release pressure, in receiving tank, cooling 10min opens the material outlet valve with the material explosion, collect solidliquid mixture, solid-liquid separation, the solid water cleans, 45 ℃ of oven dry down, collect liquid and measure composition, the steam explosion pre-treatment dextran rate of recovery is 90%, and the xylan rate of recovery is 62%;
(3) in the cellulase of the dried dry thing of 1g, 12FPU and the ratio of 12g water dry thing and cellulase are added in the water, with 1M sodium citrate buffer solution regulator solution pH=4.5, at 50 ℃, enzyme digestion reaction 96h in the 200rpm shaking table, solidliquid mixture is centrifugal 10min under 12000rpm, obtain being rich in the liquid of glucose and wood sugar, enzymolysis dextran transformation efficiency is 95%, and the xylan transformation efficiency is 86%.
Embodiment 4
A kind of method that improves lignocellulose enzymatic saccharification efficient comprises the steps:
(1) rice straw that will newly gather in is dried to that to contain quality be 75%, pulverizes with pulverizer then, and making length is 0.7 ± 0.2cm, is that 150g/L is encapsulated in the sealing bag with the density of lignocellulosic material dry weight, stores 12 months;
(2) material that step (1) is obtained joins in the retort of intermittent type steam explosion reactor, close the material inlet valve, feed steam heating, to temperature of reaction be 220 ℃, keep 3min, abrupt release pressure, with the material explosion to steam explosion in receiving tank, the cooling 10min, open the material outlet valve, collect solidliquid mixture, solid-liquid separation, the solid water cleans, 45 ℃ of oven dry down, collect liquid and measure composition, the steam explosion pre-treatment dextran rate of recovery is 95%, and the xylan rate of recovery is 68%;
(3) in the cellulase of the dried dry thing of 1g, 18FPU and the ratio of 9g water dry thing and cellulase are added in the water, with 1M sodium citrate buffer solution regulator solution pH=5.0, at 50 ℃, enzyme digestion reaction 36h in the 200rpm shaking table, solidliquid mixture is centrifugal 10min under 12000rpm, obtain being rich in the liquid of glucose and wood sugar, enzymolysis dextran transformation efficiency is 91%, and the xylan transformation efficiency is 84%.
Claims (10)
1. a method that improves lignocellulose enzymatic saccharification efficient is characterized in that comprising the steps:
(1) lignocellulosic material that will contain quality 30%-75% is pulverized, and is encapsulated in the tightness system storage;
(2) material that step (1) is obtained joins in the steam explosion reactor, uses steam heating, and abrupt release pressure obtains solidliquid mixture, and with the solidliquid mixture solid-liquid separation, solid washes with water, drying;
(3) dry thing adds cellulase and water, transfers pH=4.5-5.5, enzymolysis, and solid-liquid separation again obtains being rich in the liquid of glucose and wood sugar.
2. method according to claim 1, it is characterized in that described step (1) is: will contain the lignocellulosic material that quality is 30%-75%, it is long to be crushed to 0.5cm-4.0cm, is that 50g/L-150g/L is encapsulated in the tightness system with the density of lignocellulosic material dry weight, storage 1-12 month.
3. method according to claim 1, it is characterized in that described step (2) is: the material that step (1) is obtained joins in the steam explosion reactor, with being steam heated to 170 ℃-220 ℃, keep 3-10min, abrupt release pressure, obtain solidliquid mixture, with the solidliquid mixture solid-liquid separation, solid washes with water, drying.
4. method according to claim 1, it is characterized in that described step (3) is: the ratio in the dry thing of 1g, 5FPU-36FPU cellulase and 9-24g water is added to the water dry thing, cellulase, transfer pH=4.5-5.5, enzymolysis 36-168h, solid-liquid separation again obtains being rich in the liquid of glucose and wood sugar.
5. method according to claim 1 and 2 is characterized in that the quality that contains of described lignocellulosic material is 35%-60%.
6. according to claim 1,2 or 5 described methods, it is characterized in that described lignocellulosic material is maize straw, broomcorn straw, wheat stalk or rice straw.
7. method according to claim 1 and 2 is characterized in that the length that described lignocellulose is pulverized is 1.0cm-3.0cm.
8. method according to claim 2 is characterized in that density is 60g/L-120g/L.
9. method according to claim 2, the time that it is characterized in that described storage is 3-9 month.
10. method according to claim 3 is characterized in that steam-heated temperature is 190 ℃-210 ℃, keeps 5min-8min.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073532A (en) * | 2014-06-09 | 2014-10-01 | 华南理工大学 | Method for preparing xylose and glucose by pre-treating bagasse materials |
| CN105567744A (en) * | 2016-01-21 | 2016-05-11 | 天津大学 | Method for improving utilization rate of xylose in lignocellulose hydrolysate |
| CN112458127A (en) * | 2020-12-04 | 2021-03-09 | 浙江华康药业股份有限公司 | Method for improving high-solid-state enzymolysis rate of biomass |
| CN114098053A (en) * | 2021-12-08 | 2022-03-01 | 福建农林大学 | Preparation method of edible fungus leftover instant powder |
| CN114210714A (en) * | 2021-11-05 | 2022-03-22 | 新疆冠农果茸股份有限公司 | Anaerobic fermentation method capable of improving utilization rate of waste straws |
| CN114454280A (en) * | 2022-02-15 | 2022-05-10 | 浙江农林大学 | Method for adjusting chromatic aberration of wood veneer |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101338333A (en) * | 2008-08-12 | 2009-01-07 | 南京林业大学 | Method for conversing agricultural and forest residues to be fermentable sugars |
| CN102605020A (en) * | 2012-03-29 | 2012-07-25 | 天津大学 | Method for improving lignocellulose enzymolysis and saccharification efficiency |
-
2013
- 2013-05-08 CN CN2013101668148A patent/CN103243136A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101338333A (en) * | 2008-08-12 | 2009-01-07 | 南京林业大学 | Method for conversing agricultural and forest residues to be fermentable sugars |
| CN102605020A (en) * | 2012-03-29 | 2012-07-25 | 天津大学 | Method for improving lignocellulose enzymolysis and saccharification efficiency |
Non-Patent Citations (2)
| Title |
|---|
| LIU, ZH.H.等: "Effects of biomass particle size on steam explosion pretreatment performance for improving the enzyme digestibility of corn stover", 《INDUSTRIAL CROPS AND PRODUCTS》 * |
| LIU, ZH.H.等: "Evaluation of storage methods for the conversion of corn stover biomass to sugars based on steam explosion pretreatment", 《 BIORESOURCE TECHNOLOGY》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073532A (en) * | 2014-06-09 | 2014-10-01 | 华南理工大学 | Method for preparing xylose and glucose by pre-treating bagasse materials |
| CN105567744A (en) * | 2016-01-21 | 2016-05-11 | 天津大学 | Method for improving utilization rate of xylose in lignocellulose hydrolysate |
| CN112458127A (en) * | 2020-12-04 | 2021-03-09 | 浙江华康药业股份有限公司 | Method for improving high-solid-state enzymolysis rate of biomass |
| CN114210714A (en) * | 2021-11-05 | 2022-03-22 | 新疆冠农果茸股份有限公司 | Anaerobic fermentation method capable of improving utilization rate of waste straws |
| CN114098053A (en) * | 2021-12-08 | 2022-03-01 | 福建农林大学 | Preparation method of edible fungus leftover instant powder |
| CN114454280A (en) * | 2022-02-15 | 2022-05-10 | 浙江农林大学 | Method for adjusting chromatic aberration of wood veneer |
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Application publication date: 20130814 |