CN102261004B - Biological-chemical combined pretreatment method of lignocellulosic materials - Google Patents
Biological-chemical combined pretreatment method of lignocellulosic materials Download PDFInfo
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Abstract
The invention belongs to the technical field of biochemical engineering and particularly relates to a method and system for carrying out combined pretreatment on lignocellulosic materials by combining microbiological treatment and dilute acid treatment. The method is characterized by grinding the lignocellulosic materials to 5-60 meshes, then putting the ground lignocellulosic materials in a microbiological treatment tank, and injecting microbiological bacteria liquid for biological treatment for 5-10 days; and putting the materials subjected to microbiological treatment in a dilute acid recycle reactor, injecting dilute acid, and starting a circulating pump to carry out circulating treatment at 80-100 DEG C for 1-4 hours, thus completing pretreatment. The method has the advantages of lowacid consumption, mild operation conditions, low requirements for compression resistance and corrosion resistance of the reactor, low treatment cost, less secondary pollution, environment friendliness and the like.
Description
Technical field
The invention belongs to technical field of biochemical industry, be specifically related to a kind of employing microbiological treatment and diluted acid and handle to combine and carry out lignocellulosic material and unite pretreated method and system thereof.
Background technology
Lignocellulosic material is through behind the enzymatic saccharification, and can obtain hexose based on glucose and reach pentose based on wood sugar, be the important source material of industries such as food and chemical industry.Lignocellulosic material mainly is made up of cellulose, hemicellulose and lignin three big compositions, hemicellulose is combined between cellulose and the lignin as the molecule binder, cellulosic molecule is embedded in wherein, form a kind of natural cover for defense, make enzyme be difficult for contacting with cellulosic molecule, cause in the lignocellulosic material enzymolysis of polysaccharide slow.Therefore the enzymatic saccharification that will realize lignocellulosic material must effectively separate its three kinds of compositions by suitable preconditioning technique, interrupt the part glycosidic bond simultaneously, reduce the degree of polymerization, remove lignin, improve the natural structure of lignocellulosic material, releasing strengthens enzyme and cellulose accessibility to the barrier inhibition of enzyme, improves cellulase hydrolysis saccharification efficient.Therefore suitable preprocess method is one of lignocellulosic material trans-utilization key link.
At present, the pretreated method of lignocellulosic material mainly contains physics method, chemical method and bioanalysis.Physics, chemical pretreatment techniques have several different methods such as mechanical pulverization process, steam blasting processing, microwave and ultrasonic wave processing, radiation treatment, alkaline process processing, peroxide oxidation processing, diluted acid immersion treatment, organic solvent processing, ammonia explosion treatment, carbon dioxide explosion treatment, wet oxidation process.But multiple reasons such as a lot of physical chemistry methods wherein are high because of poor processing effect, environmental pollution, equipment cost and operating cost, complicated operation are not promoted, and wherein comparatively maturation method mainly contains steam explosion, two kinds of methods of diluted acid infusion method.
Steam explosion is the most frequently used method of a kind of lignocellulosic material preliminary treatment, under the situation that does not add catalyst, utilize high steam to heat up rapidly, with raw material and water or steam etc. at high temperature, after (150～240 ℃) handle certain hour under the high pressure, be down to normal temperature immediately, normal pressure, in the steam blasting process, high steam infiltrates to fibrous inside, mode with air-flow discharges from the blind bore crack, make fiber that certain mechanical breaking take place, simultaneously high temperature, the destruction of the inner hydrogen bond of high pressure aggravation cellulose, the free hydroxyl that makes new advances, ordered structure in the cellulose changes, and has increased cellulosic adsorption capacity.But because the acidity of the acetic acid that produces in the steam blasting process and some other acid is very weak, it is not very high causing double cellulosic changing effect, generally only be about 65～70%, and acetic acid also is a kind of inhibition product of alcohol fermentation; Sugar monomer after the hemicellulose degraded at high temperature can be converted into fermentation inhibitors such as furfural, hydroxymethylfurfural, and raw material need also cause the loss of sugared composition simultaneously with big water gaging flushing; Steam explosion is very low to lignin removing rate, and the lignin behind steam blasting has higher non-specific adsorption to cellulase, and some oligosaccharides and fracture lignin can attach to cellulose surface at the cooling postprecipitation simultaneously, hinder cellulase hydrolysis.
The diluted acid infusion method also is to use comparatively ripe preprocess method at present.Refer to lignocellulosic material to be handled being below or above under 100 ℃ the temperature with the diluted acid (0.5～1%) of low concentration.When carrying out preliminary treatment under being lower than 100 ℃ temperature, the processing time is generally 4～24h; When carrying out preliminary treatment under being higher than 100 ℃ temperature, general a few minutes in processing time were to several hours.The dilute acid hydrolysis hemicellulose also changes the cellulose crystal formation, increases its specific area, improves follow-up enzymolysis efficiency.But the reaction time of diluted acid infusion method is longer, the energy consumption height, and the hemicellulose conversion ratio is not high yet, is generally 70～80%; The diluted acid infusion method does not remove substantially to lignin, can not efficient solution remove lignin to ineffective adsorption and the steric restriction of cellulase, suppress product because the long reaction time causes sugar monomer further to generate fermentations such as furfural, formic acid, acetic acid, hydroxymethylfurfural simultaneously, influence follow-up enzymolysis, fermentation.
Bioanalysis mainly utilizes the biocatalysis (comprising fungi, bacterium, actinomyces etc.) of microorganism to carry out the degraded of components such as lignin, hemicellulose, cellulose, improves follow-up cellulose, hemicellulose saccharification efficient.This method reaction condition gentleness, not causing advantages such as environmental pollution, cost be low, is the focus of research both at home and abroad at present.But report that from domestic and international research there are shortcomings such as length, low, the sugared composition loss of efficient of pretreatment time in bioanalysis.
Problem in view of above preconditioning technique existence, press for and seek a kind of new preprocess method and to obtain higher hemicellulose conversion ratio, less inhibition product and to remove lignin barrier inhibition and the ineffective adsorption of cellulase substituted existing method, raising lignocellulosic material enzymolysis efficiency.
Summary of the invention
For overcoming the problem that above-mentioned physics method, chemical method and bioanalysis pretreating process exist, the invention provides a kind of method that can effectively improve hemicellulose conversion ratio in the lignocellulosic material, delignification rate and cellulase hydrolysis conversion coefficient and reduce fermentation inhibition product.
Another object of the present invention is to provide a kind of coupling system for carrying out said process.
The present invention can realize in the following manner:
A kind of biological-chemical combined pretreatment method of lignocellulosic material, lignocellulosic material is crushed in the microbiological treatment pond of packing into after 5～60 purpose granularities, injecting microbial inoculum carries out a biological disposal upon, microorganism used therefor bacterium liquid is efficient lignin degradation microorganism, and the biological treatment condition is: press 5～10% of lignocellulosic material dry weight, add microbial inoculum, add water to the reaction system moisture content and reach 65～75%, 25～35 ℃ of temperature are handled 5～10d, and blow vent is opened.
Described lignocellulosic material is mainly maize straw, wheat stalk and rice straw.
Described microbial inoculum mainly comprise bristle bolt bacterium (
Trametes trogii) bacterium numbering: CGMCC NO.3772; Variegated bolt bacterium (
Trametes versicolor) bacterium numbering: CGMCC NO.3773, this two strains bacterial classification on April 23rd, 2010 in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101.
The above-mentioned raw material of crossing through microbiological treatment is packed in the diluted acid recirculation reactor, and injection diluted acid, opening circulating pump is to carry out circular treatment under 80～100 ℃ in temperature, being about to react filtrate ceaselessly extracts out and is back to again and carry out circular treatment in the reactor, the circular treatment time is generally 1～4h, finishes preliminary treatment.
After reaction finishes the treatment fluid that obtains is collected, neutralization deacidifies, filters the back and is used for later use.
The mass fraction of described diluted acid is 0.1～2.0% dilute sulfuric acid; The addition of diluted acid is: the weight ratio of lignocellulosic material dry weight and pickle is 1:5～20.
After the diluted acid circular treatment finished, remaining lignocellulosic material water flushing back was taken out and is used for follow-up enzymolysis in the reactor, namely removes residual diluted acid and lignin with warm water washing earlier, took out subsequently to be used for follow-up enzymolysis.
A kind of biological-chemical associating pretreatment system of lignocellulosic material mainly is made up of microbiological treatment pond, diluted acid recirculation reactor, acid storage tank, collecting tank and material conveyor.The microbiological treatment pond mainly comprises seeding tank and spray line, charging aperture, blow vent and discharging opening, seeding tank is located at the top for the treatment of pond, spray line is located at the top for the treatment of pond, be communicated with seeding tank by pipeline, charging aperture and discharging opening are located at the upper and lower for the treatment of pond respectively, and a plurality of blow vent branches are located at the treatment pond bottom; The choosing of diluted acid recirculation reactor mainly comprises charging aperture, discharging opening, tapping valve, spray head, filter plate and circulating pump, charging aperture and discharging opening are located at the reactor upper and lower, tapping valve is located at reactor bottom, spray head is located at reactor head, filter plate is located at the junction of reactor column part and hemispherical portion, circulating pump is installed in the reactor outside, and the entrance of circulating pump is communicated with by pipeline with reactor bottom, and outlet of circulating pump is communicated with by pipeline with the spray head that is located at reactor head; Acid storage tank is communicated with diluted acid recirculation reactor top by pipeline; Collecting tank is communicated with the diluted acid reactor bottom by pipeline; Material conveyor is installed between microbiological treatment pond and the diluted acid recirculation reactor, and microbiological treatment pond discharging opening is communicated with diluted acid recirculation reactor charging aperture by pipeline.
This system also comprises and is located at diluted acid reactor head hot-water line and is located at bottom discharging of waste liquid pipe.
Above-mentioned circulating pump is the centrifugal pump of acid corrosion-resistant.
Biological-chemical combined pretreatment method provided by the invention passes through first step microbiological treatment the lignin in the lignocellulosic material, hemicellulose and cellulose composition small amount of degradation, reduce lignin and hemicellulose to cellulosic package action, make raw material loose, softening, thus be beneficial to the infiltration of follow-up pickle; Raw material after microbiological treatment enters the second step diluted acid circular treatment, by the pickle circulation static acid soak processing mode is become dynamic processing mode.Static acid soak processing mode namely leaves standstill batch formula reaction, like this after lignocellulosic material is handled a period of time, catabolite concentration around it can exceed much than other places, be unfavorable for that like this direction of reacting to catabolite carries out, treatment effeciency is limited, and near the catabolite the lignocellulosic material is taken away in the continuous circulation that the diluted acid circular treatment can be passed through, be diffused in the whole reaction system and go, make to remain lower catabolite near the lignocellulosic material, be conducive to improve treatment effeciency; Simultaneously, adopt dynamic circulation to handle the catabolite that can break up some slightly solubilities that are blocked in the lignocellulosic material surface, make acid solution to contact that with lignocellulosic material it is carried out catalytic reaction better, accelerate the degraded of lignocellulose raw material.Therefore can effectively improve the treatment effeciency of lignocellulosic material after microbiological treatment and the diluted acid circulation associating.
Biological-chemical associating pretreatment system provided by the invention is made up of microbiological treatment pond, material conveyor and diluted acid recirculation reactor three parts.The microbial pretreatment pond is preferably square, and the upper and lower is respectively equipped with charging aperture and discharging opening, and the bottom is also set up a plurality of blow vents separately simultaneously, sufficient oxygen supply during with the assurance microbiological treatment; The top is provided with seeding tank, be communicated with spray line in the microbiological treatment pond by pipeline, spray function by spray line can guarantee that strain liquid is evenly distributed on and be beneficial to inoculation on the lignocellulosic material evenly, can also carry out raw material moisture by spray line simultaneously and regulate and replenish.Material conveyor adopts the spiral pusher mode, is installed between microbiological treatment pond discharging opening and the diluted acid recirculation reactor charging aperture, and the raw material after the microbiological treatment is transported to the diluted acid recirculation reactor.The diluted acid recirculation reactor is preferably cylindrical, and the upper and lower is respectively equipped with charging aperture and discharging opening; The top is planar structure, the bottom is hemispherical dome structure, the top is provided with spray head and links to each other with external pipe, the diafiltration reactant liquor passes back into reactor by the effect of circulating pump through spray head during preliminary treatment, spray head is except allowing percolate be distributed in uniformly on the lignocellulosic material to be conducive to the reaction, can also suppress moisture evaporation, guarantee that reaction system is even; The below of reactor is provided with a filter plate with holes, and filter plate can partly be blocked in raw material solid in the reactor, and liquid partly enters the lower hemisphere space, guarantees to handle to continue to circulate to carry out.
The present invention has the following advantages:
1, the present invention is by the associating of microbiological treatment and diluted acid circular treatment, can realize both effective supplements, microbiological treatment has reduced lignin and hemicellulose to cellulosic package action, make raw material loose, softening, thereby be beneficial to the infiltration of follow-up pickle, improve the efficient of diluted acid circular treatment;
2, compare with traditional dilute acid pretreatment method, this method acid consumption significantly reduces, operating condition gentleness, to the resistance to compression of reactor with corrosion resistance is less demanding, processing cost is low, secondary pollution is few, environmental friendliness;
3, compare with traditional dilute acid pretreatment method, the diluted acid circulation of this method can flow into the product such as pentose that generate rapidly in the pipeline of circulation by the continuous circulation of diluted acid reactant liquor, and the liquid glucose temperature is reduced rapidly, reduces the generation of accessory substances such as furfural.
In a word, compare with the pre existing treatment technology, the present invention has that acid consumption is few, operating condition is gentle, to the resistance to compression of reactor with corrosion resistance is less demanding, processing cost is low, secondary pollution is few and advantages of environment protection, the accessory substance of Sheng Chenging is few simultaneously, significant loss is low.By microbiological treatment and diluted acid circular treatment associating complementation, solved under the operating condition and low pollution of gentleness, the technical barrier of efficiently preprocessing lignocellulose raw material, for taking full advantage of of lignocellulosic material opened up new way, has great application prospect in fields such as bioenergy, bio-based chemicals, industrial fermentations.
Description of drawings
Accompanying drawing is the structural representation of biological-chemical associating pretreatment system of the present invention.
1-8 is valve among the figure, the 9th, and acid storage tank, the 10th, collecting tank, the 11st, diluted acid recirculation reactor, the 12nd, diluted acid recirculation reactor charging aperture, the 13rd, diluted acid recirculation reactor discharging opening, the 14th, spray head, the 15th, filter plate, the 16th, circulating pump, the 17th, material conveyer, the 18th, seeding tank, the 19th, microbiological treatment pond, the 20th, microbiological treatment pond discharging opening, the 21st, microbiological treatment pond charging aperture, the 22nd, blow vent, the 23rd, bacterial classification spray line.
The specific embodiment
Embodiment 1: the maize straw (cellulose 33.31%, hemicellulose 26.82%, lignin 21.46%) that will dry constant weight is crushed to behind 10 orders from charging aperture 20 is packed microbiological treatment pond 19 into, open valve 8, from seeding tank 18, inject cultured hair bolt bacterium strain liquid, inoculum concentration 5% (percentage by weight, dry weight), transfer moisture content to 65%, open blow vent 22, handle 5d down at 30 ℃.Open discharging opening 20, open raw material that material conveyer 17 crosses microbiological treatment from charging aperture 12 is packed diluted acid recirculation reactor 11 into, open valve 1, valve 2, the dilute sulfuric acid of injection 0.5% is in reactor 11 from acid storage tank 9, and the solid-to-liquid ratio of maize straw and dilute sulfuric acid is 1:10; Open valve 3, valve 4, valve-off 1, valve 2, start circulating pump 16, at 90 ℃ of following circular response 2h, stage of reaction interstitial fluid body portion circulates by the Separation of Solid and Liquid effect of filter plate 15, solid material part is separated by filter plate 15 and is stopped and stays in the diluted acid recirculation reactor 11, and liquid partly extracts through circulating pump 16 and is promoted to spray head 14 and disperses to be back in the diluted acid recirculation reactor 11 in the mode of spray; After reaction finished, valve-off 4 was opened charging aperture 12 and valve 6, and processing reaction liquid flows in the collecting tank 10, and the neutralization back is used for later use; Valve-off 6 is opened valve 1, valve 5, and the solid in the reactor is stayed in water flowing (HP) flushing, and foreign material such as the residual diluted acid of flush away, lignin and carbohydrate, waste liquid (WL) are discharged through valve 5 and carried out subsequent treatment; Open discharging opening 20 at last, remaining solid is taken out, be used for follow-up enzymatic saccharification.Half fiber conversion ratio reaches 90.5%, and xylose concentration is 20.6g/L; Lignin removing rate reaches 11.4%; Pretreated solid material is enzymolysis 60h(50 ℃, pH4.8, solid-to-liquid ratio 1:30 under cellulase consumption 30FPU/g effect), conversion coefficient reaches 72.3%.
Embodiment 2: the maize straw (cellulose 33.31%, hemicellulose 26.82%, lignin 21.46%) that will dry constant weight is crushed to behind 10 orders from charging aperture 20 is packed microbiological treatment pond 19 into, open valve 8, from seeding tank 18, inject cultured variegated bolt bacterium strain liquid, inoculum concentration 5% (percentage by weight, dry weight), transfer moisture content to 65%, open blow vent 22, handle 5d down at 30 ℃.Open discharging opening 20, open raw material that material conveyer 17 crosses microbiological treatment from charging aperture 12 is packed diluted acid recirculation reactor 11 into, open valve 1, valve 2, the dilute sulfuric acid of injection 0.5% is in reactor 11 from acid storage tank 9, and the solid-to-liquid ratio of maize straw and dilute sulfuric acid is 1:10; Open valve 3, valve 4, valve-off 1, valve 2, start circulating pump 16, at 90 ℃ of following circular response 2h, stage of reaction interstitial fluid body portion circulates by the Separation of Solid and Liquid effect of filter plate 15, solid material part is separated by filter plate 15 and is stopped and stays in the diluted acid recirculation reactor 11, and liquid partly extracts through circulating pump 16 and is promoted to spray head 14 and disperses to be back in the diluted acid recirculation reactor 11 in the mode of spray; After reaction finished, valve-off 4 was opened charging aperture 12 and valve 6, and processing reaction liquid flows in the collecting tank 10, and the neutralization back is used for later use; Valve-off 6 is opened valve 1, valve 5, and the solid in the reactor is stayed in water flowing (HP) flushing, and foreign material such as the residual diluted acid of flush away, lignin and carbohydrate, waste liquid (WL) are discharged through valve 5 and carried out subsequent treatment; Open discharging opening 20 at last, remaining solid is taken out, be used for follow-up enzymatic saccharification.Half fiber conversion ratio reaches 78.6%, and xylose concentration is 17.4g/L; Lignin removing rate reaches 8.5%; Pretreated solid material is enzymolysis 60h(50 ℃, pH4.8, solid-to-liquid ratio 1:30 under cellulase consumption 30FPU/g effect), conversion coefficient reaches 62.8%.
Embodiment 3: the maize straw (cellulose 33.31%, hemicellulose 26.82%, lignin 21.46%) that will dry constant weight is crushed to behind 10 orders from charging aperture 20 is packed microbiological treatment pond 19 into, open valve 8, from seeding tank 18, inject cultured hair bolt bacterium strain liquid, inoculum concentration 5% (percentage by weight, dry weight), transfer moisture content to 65%, open blow vent 22, handle 5d down at 30 ℃.Open discharging opening 20, open raw material that material conveyer 17 crosses microbiological treatment from charging aperture 12 is packed diluted acid recirculation reactor 11 into, open valve 1, valve 2, the dilute sulfuric acid of injection 0.5% is in reactor 11 from acid storage tank 9, and the solid-to-liquid ratio of maize straw and dilute sulfuric acid is 1:10; Open valve 3, valve 4, valve-off 1, valve 2, start circulating pump 16, at 85 ℃ of following circular response 2h, stage of reaction interstitial fluid body portion circulates by the Separation of Solid and Liquid effect of filter plate 15, solid material part is separated by filter plate 15 and is stopped and stays in the diluted acid recirculation reactor 11, and liquid partly extracts through circulating pump 16 and is promoted to spray head 14 and disperses to be back in the diluted acid recirculation reactor 11 in the mode of spray; After reaction finished, valve-off 4 was opened charging aperture 12 and valve 6, and processing reaction liquid flows in the collecting tank 10, and the neutralization back is used for later use; Valve-off 6 is opened valve 1, valve 5, and the solid in the reactor is stayed in water flowing (HP) flushing, and foreign material such as the residual diluted acid of flush away, lignin and carbohydrate, waste liquid (WL) are discharged through valve 5 and carried out subsequent treatment; Open discharging opening 20 at last, remaining solid is taken out, be used for follow-up enzymatic saccharification.Half fiber conversion ratio reaches 82.2%, and xylose concentration is 18.9g/L; Lignin removing rate reaches 9.7%; Pretreated solid material is enzymolysis 60h(50 ℃, pH4.8, solid-to-liquid ratio 1:30 under cellulase consumption 30FPU/g effect), conversion coefficient reaches 66.8%.
Embodiment 4: the maize straw (cellulose 33.31%, hemicellulose 26.82%, lignin 21.46%) that will dry constant weight is crushed to behind 10 orders from charging aperture 20 is packed microbiological treatment pond 19 into, open valve 8, from seeding tank 18, inject cultured hair bolt bacterium strain liquid, inoculum concentration 5% (percentage by weight, dry weight), transfer moisture content to 65%, open blow vent 22, handle 5d down at 30 ℃.Open discharging opening 20, open raw material that material conveyer 17 crosses microbiological treatment from charging aperture 12 is packed diluted acid recirculation reactor 11 into, open valve 1, valve 2, the dilute sulfuric acid of injection 0.5% is in reactor 11 from acid storage tank 9, and the solid-to-liquid ratio of maize straw and dilute sulfuric acid is 1:10; Open valve 3, valve 4, valve-off 1, valve 2, start circulating pump 16, at 95 ℃ of following circular response 1h, stage of reaction interstitial fluid body portion circulates by the Separation of Solid and Liquid effect of filter plate 15, solid material part is separated by filter plate 15 and is stopped and stays in the diluted acid recirculation reactor 11, and liquid partly extracts through circulating pump 16 and is promoted to spray head 14 and disperses to be back in the diluted acid recirculation reactor 11 in the mode of spray; After reaction finished, valve-off 4 was opened charging aperture 12 and valve 6, and processing reaction liquid flows in the collecting tank 10, and the neutralization back is used for later use; Valve-off 6 is opened valve 1, valve 5, and the solid in the reactor is stayed in water flowing (HP) flushing, and foreign material such as the residual diluted acid of flush away, lignin and carbohydrate, waste liquid (WL) are discharged through valve 5 and carried out subsequent treatment; Open discharging opening 20 at last, remaining solid is taken out, be used for follow-up enzymatic saccharification.Half fiber conversion ratio reaches 92.6%, and xylose concentration is 21.1g/L; Lignin removing rate reaches 14.7%; Pretreated solid material is enzymolysis 60h(50 ℃, pH4.8, solid-to-liquid ratio 1:30 under cellulase consumption 30FPU/g effect), conversion coefficient reaches 73.8%.
Claims (6)
1. the biological-chemical combined pretreatment method of a lignocellulosic material, it is characterized in that: lignocellulosic material is crushed in the microbiological treatment pond of packing into after 5～60 purpose granularities, injects bristle bolt bacterium Trametes trogii CGMCC NO.3772 or the efficient lignin degradation microbial inoculum of the variegated bolt bacterium Trametes versicolor CGMCC NO.3773 5～10d that carries out a biological disposal upon; To pack in the diluted acid recirculation reactor through the raw material that microbiological treatment is crossed, and the implantation quality mark is 0.1～2.0% dilute sulfuric acid that opening circulating pump is to carry out circular treatment 1～4h under 80～100 ℃ in temperature, finishes preliminary treatment.
2. the biological-chemical combined pretreatment method of a kind of lignocellulosic material according to claim 1, it is characterized in that: described biological treatment is: press 5～10% of lignocellulosic material dry weight, add microbial inoculum, add water to the reaction system moisture content and reach 65～75%, 25～35 ℃ of temperature, handle 5～10d, blow vent is opened.
3. the biological-chemical combined pretreatment method of a kind of lignocellulosic material according to claim 1, it is characterized in that: the addition of diluted acid is: the weight ratio of lignocellulosic material dry weight and pickle is 1:5～20.
4. the biological-chemical of lignocellulosic material associating pretreatment system comprises microbiological treatment pond, diluted acid recirculation reactor, acid storage tank, collecting tank and material conveyor; The microbiological treatment pond comprises seeding tank and spray line, charging aperture, blow vent and discharging opening, seeding tank is located at the top for the treatment of pond, spray line is located at the top for the treatment of pond, be communicated with seeding tank by pipeline, charging aperture and discharging opening are located at the upper and lower for the treatment of pond respectively, and a plurality of blow vent branches are located at the treatment pond bottom; The diluted acid recirculation reactor comprises charging aperture, discharging opening, tapping valve, spray head, filter plate and circulating pump, charging aperture and discharging opening are located at the reactor upper and lower, tapping valve is located at reactor bottom, spray head is located at reactor head, filter plate is located at the junction of reactor column part and hemispherical portion, circulating pump is installed in the reactor outside, and the entrance of circulating pump is communicated with by pipeline with reactor bottom, and outlet of circulating pump is communicated with by pipeline with the spray head that is located at reactor head; Acid storage tank is communicated with diluted acid recirculation reactor top by pipeline; Collecting tank is communicated with the diluted acid reactor bottom by pipeline; Material conveyor is installed between microbiological treatment pond and the diluted acid recirculation reactor, and microbiological treatment pond discharging opening is communicated with diluted acid recirculation reactor charging aperture by pipeline.
5. the biological-chemical of a kind of lignocellulosic material according to claim 4 is united pretreatment system, it is characterized in that: this system also comprises the hot-water line that is located at the diluted acid reactor head and is located at the discharging of waste liquid pipe of bottom.
6. according to the biological-chemical associating pretreatment system of claim 4 or 5 described a kind of lignocellulosic materials, it is characterized in that: described circulating pump is the centrifugal pump of acid corrosion-resistant.
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| CN102839116A (en) * | 2012-09-11 | 2012-12-26 | 中国科学院成都生物研究所 | System and method for producing methane through fermenting of mixed raw material |
| CN104611391B (en) * | 2013-11-05 | 2018-08-14 | 中国石油化工股份有限公司 | A kind of method that lignocellulosic material prepares hydrolysis sugar |
| CN105177055B (en) * | 2015-07-31 | 2018-10-09 | 湘潭大学 | A kind of biological-chemical combined treatment process improving lignocellulosic saccharification result |
| CN105753529A (en) * | 2016-03-08 | 2016-07-13 | 山东师范大学 | Compost treatment device and method of city domestic sludge and straw |
| CN110761103A (en) * | 2019-10-30 | 2020-02-07 | 北京地山谦生物科技有限公司 | Biological papermaking pulping system |
| CN113293104A (en) * | 2020-02-21 | 2021-08-24 | 北京威力格生物科技有限公司 | Fungus polysaccharide and preparation method and application thereof |
| CN111809429A (en) * | 2020-07-24 | 2020-10-23 | 淄博圣泉纸业有限公司 | Pulping method with biological enzyme cooperation |
| CN116640707A (en) * | 2023-07-27 | 2023-08-25 | 中国农业科学院农业环境与可持续发展研究所 | Method for strengthening agricultural waste composting by combining acid pretreatment and microorganism |
| CN117925768B (en) * | 2024-03-19 | 2024-06-14 | 北京林业大学 | Combined pretreatment method for improving lignocellulose degradation and sugar conversion rate |
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