CN108148879A - A kind of preprocess method of maize straw - Google Patents
A kind of preprocess method of maize straw Download PDFInfo
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- CN108148879A CN108148879A CN201810176982.8A CN201810176982A CN108148879A CN 108148879 A CN108148879 A CN 108148879A CN 201810176982 A CN201810176982 A CN 201810176982A CN 108148879 A CN108148879 A CN 108148879A
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- stalk
- maize straw
- straw
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention discloses a kind of preprocess method of maize straw, this method is by maize straw after crushing or mechanical cutoff, adds Ca (OH)2And water, sealing and standing is handled after mixing, is passed through carbon dioxide later and is neutralized.Treated stalk, content of reducing sugar reaches as high as 0.7g/g dry straws after enzymolysis, is more than the 90% of theoretical value.Pretreated straw is easily biological trans-utilization, has larger use value.Calcium hydroxide is as a kind of substance for being slightly soluble in water, in Ca2+It is sustainable that leniently lignocellulosic is modified constantly during dissociation release, achieve the purpose that abolish its structural barriers, stalk structure is made to become loose, effectively improve the utilization rate of stalk in production.This method is with easy to operate, mild condition, and enzymolysis efficiency is high, and inorganic agent is cheap convenient for recycling, sewage is not generated, suitable for industrial mass processing.
Description
Technical field
The invention belongs to applied technical fields, and in particular to a kind of preprocess method of maize straw.
Background technology
Corn is one of Chinese staple crops, and annual China's maize straw yield is up to 300,000,000 tons or so.Stalk mainly by
Three kinds of cellulose, hemicellulose and lignin are into being grouped as.Three is combined together to form by different degrees of crosslinked action
Stalk extremely firm structure hinders the efficient enzymolysis of stalk.Cellulose, hemicellulose in stalk can be converted into sugar
It is further converted to multiple product after class, and lignin hinders cellulose, hemicellulose efficiently uses.In order to effectively sharp
With stalk cellulose, it is necessary first to which stalk is pre-processed.The method of pretreated straw has physics pretreatment at present, chemistry
Pretreatment and Biological Pretreatment three classes.Wherein Physical has attrition grinding method, steam explosion；Chemical method mainly includes
Sour processing, alkali process, oxidant and ionic liquid processing；Bioanalysis is handled lignocellulosic using fungi.
Related patents have：A kind of raising gramineae stalk anaerobic digestion gas production effect of 1061192 A of Publication No. CN
The preprocess method of rate carries out 30d anaerobic fermentations after impregnating 40 mesh stalk pretreatments using 10% hydrochloric acid and 10% ammonium hydroxide.It is public
The number of opening is a kind of method of High-efficiency straw producing methane of 105420281 A of CN, utilizes the aqueous solution soaking of metal salt catalyst
Pretreated liquid portion is subjected to biogas fermentation after stalk.Physical-chemical-life of 105255953 A of Publication No. CN
The method that object pre-processes maize straw combines 1% sodium hydroxide and mould processing stalk progress stalk ingredient point using crushing
Analysis.Publication No. CN 106119295A's utilizes NH4HCO3To pre-process the anaerobism of the method for maize straw and maize straw hair
Fermenting process, using NH4HCO3Processing maize straw has simultaneously carried out anaerobic fermentation to stalk after processing.
More than invention carries out anaerobic fermentation again after being pre-processed to stalk, shorten anaerobic fermentation to a certain extent
Period, improve gas production, but all exist and operate more numerous and diverse, due to needing to add bronsted lowry acids and bases bronsted lowry, security risk is high,
Sewage can be also generated in preprocessing process leads to secondary pollution etc..Therefore, straw pretreatment method needs new breakthrough.
Invention content
The purpose of the present invention is to provide a kind of preprocess method of maize straw, this method can effectively improve the enzyme of raw material
Efficiency is solved, and easy to operate, mild condition, inorganic agent additive amount are low, no contaminated wastewater, convenient for the industrialization of maize straw
Using.
For achieving the above object, the present invention takes following technical measures：
A kind of preprocess method of maize straw, includes the following steps：Maize straw is after pulverizing and sieving or blocking, addition
Ca(OH)2And water, maize straw and Ca (OH)2Mass ratio be 100：1-10；Final content of water in system 50-85%, after mixing
0-50 DEG C of sealing and standing 1-10 days, is passed through the gas containing carbon dioxide to stalk pH to 6.0-8.5；
In schemes described above, it is preferred that be crushed to after the maize straw naturally dry below 10 mesh or mechanical
It blocks to below 5cm；
In schemes described above, it is preferred that maize straw and Ca (OH)2Mass ratio be 100：8, final system is aqueous
It measures as 75% (w/w).
In schemes described above, it is preferred that the temperature of sealing and standing is 25-30 DEG C, and time of repose is 6-9 days.
The gas containing carbon dioxide includes：Flue gas (the generations such as such as boiler, hot-blast stove that various burners generate
Flue gas etc.) or the tail gas (tail gas of the generations such as such as aerobic tank, anaerobic jar) that generates of fermentation tank or be passed directly into sky
Gas.
A kind of application of the processing method of maize straw prepares natural pond including the use of the stalk that method provided by the invention obtains
Gas prepares lactic acid；
In above-described application, it is preferred that be to access sludge in the stalk of preparation to carry out anaerobism when preparing biogas
Fermentation.
In above-described application, it is preferred that be that drying stalk prepared by 3g is added to 100mL's when preparing lactic acid
In the carbon-free carbon-free MRS culture mediums of MRS culture mediums, then fermented with bacillus coagulans FL209.
The present invention has the following advantages：
(1) pretreatment is lime using inorganic agent, and recyclable recycling, pretreated straw, which is not required to wash, avoids alkali process
The drawbacks of middle discharge of wastewater.
(2) pretreatment condition is mild, and without high temperature and pressure, no especial equipment requirements, low energy consumption.
(3) closed environment avoids Ca (OH)2With air CO2,H2The reaction of O improves treatment effect.
(4) it is neutralized using carbon dioxide, is conducive to the recycling of calcium hydroxide, moreover it is possible to subtract to a certain extent
Enter the carbon dioxide in air less, solve the problems, such as greenhouse effects.
(5)Ca(OH)2And CO2Generation calcium carbonate can stablize stalk fermentation system pH.
(6) sugared yield height is digested, reduced sugar is up to 0.70g/g dry straws after enzymolysis.
(7) pretreated straw biogas fermentation performance is improved well, and 15 days methane productions of unit mass stalk improve
60.82%.Pretreated straw fermenting property is improved well, and unit mass stalk 48h lactic acid producing amounts are reachable
0.415g。
Description of the drawings
Fig. 1 is pretreated maize straw fermentation cumulative gas schematic diagram.
Specific embodiment
Technical solution of the present invention if not otherwise specified, belongs to the ordinary skill in the art；The reagent or material
Material, if not otherwise specified, derives from commercial channel.
Stalk enzymolysis and its content of reducing sugar assay method used in the embodiment of the present invention is as follows：
Sample is dried, 1g samples is taken to be placed in 250mL conical flasks, adds 30U cellulases and 100mL 0.1mol/L
PH5.0 sodium citrate buffer solutions are placed in 50 DEG C of 150rpm shaking baths and digest 12h, and supernatant DNS methods is taken to survey reduced sugar.
Embodiment 1：
A kind of preprocess method of maize straw, includes the following steps：
100 kilograms of stalks (dry weight, length 0.8-1.2cm) is taken to be positioned in rustless steel container, add 8 kilograms of hydroxides
Calcium, it is 65% to add water adjustment moisture, and sealing mixing is placed at 20 DEG C, after handling 7d, is passed through aerobic fermentation tank tail gas, under
Into above going out, until stalk PH to 8.0.The enzymolysis content of reducing sugar of stalk reaches 0.55g/g stalks after processing, is theoretical value
78%, it is only 0.25g/g stalks without pretreated straw.
Embodiment 2：
A kind of preprocess method of maize straw, includes the following steps：
100 kilograms of stalk length (dry weight, 1.5cm) is taken to be positioned in rustless steel container, 2 kilograms of calcium hydroxides is added, adds
Water adjustment moisture be 55%, sealing mixing be placed at 50 DEG C, handle 10d after, be passed through anaerobism aerobe fermentation tank tail gas, under into
On go out, until stalk PH to 6.0.The enzymolysis content of reducing sugar of stalk reaches 0.40g/g stalks after processing, is the 57% of theoretical value,
It is only 0.25g/g stalks not have pretreated straw.
Embodiment 3：
A kind of preprocess method of maize straw, includes the following steps：
100 kilograms of stalks (dry weight, length is in 2.5-3.2cm) is taken to be positioned in rustless steel container, add 5 kilograms of hydroxides
Calcium, it is 50% to add water adjustment moisture, and sealing mixing is placed at 30 DEG C, after handling 5d, is passed through hot-blast stove flue flue gas, under
Into above going out, until stalk PH to 7.5.The enzymolysis content of reducing sugar of stalk reaches 0.45g/g stalks after processing, is theoretical value
64%, it is only 0.25g/g stalks without pretreated straw.
Embodiment 4：
A kind of preprocess method of maize straw, includes the following steps：
100 kilograms of stalks (dry weight, length is in 3.5-4.5cm) is taken to be positioned in rustless steel container, add 10 kilograms of hydrogen-oxygens
Change calcium, it is 60% to add water adjustment moisture, and sealing mixing is placed at 15 DEG C of room temperature, after handling 8d, is passed through hot-blast stove flue cigarette
Gas, bottom in and top out, until stalk PH to 6.5.The enzymolysis content of reducing sugar of stalk reaches 0.52g/g stalks after processing, is theoretical value
74%, be only 0.25g/g stalks without pretreated straw.
Embodiment 5：
A kind of preprocess method of maize straw, includes the following steps：
100 kilograms of stalks (length is in 2cm) is taken to be positioned in rustless steel container, 3 kilograms of calcium hydroxides is added, water is added to adjust
Moisture is 75%, and sealing mixing is placed at 10 DEG C of room temperature, after handling 3d, is passed through hot-blast stove flue flue gas, bottom in and top out,
To stalk PH to 6.5.The enzymolysis content of reducing sugar of stalk reaches 0.38g/g stalks after processing, is the 54% of theoretical value, does not have
Pretreated straw is only 0.25g/g stalks.
Embodiment 6：
A kind of application of the pretreated straw that processing method of maize straw obtains in biogas is prepared：
This experiment is divided into two groups：
Control group：Untreated maize straw biogas fermentation experiment.
Experimental group：Pretreated straw biogas fermentation is tested.
Pretreated straw preparation method is as follows：100g stalks (length is 0.5cm or so) is taken to add 8g in closed container
Calcium hydroxide, it is 75% to add water adjustment moisture, and sealing mixing is placed at 30 DEG C, and CO is led to after 7d2To stalk pH to 8.5, dry
It is dry spare.
Marsh gas fermentation processes：Take the sludge that stalk, dry weight that dry weight is 57.14g are 22.86g (dirty from Angel Yeast
Water treatment plant's anaerobic pond) add in 1L fermentation tank, 800mL is settled to water, after mixing by the initial p H tune of fermentation system
To 8.5,55 DEG C of anaerobic fermentations of 15 days are carried out, fermentation gas is collected by airbag.
Fermentation 1d, 2d, 3d, 4d, 5d, 6d, 7d, 10d, 12d, 15d utilize Portable methane analyzer Gasboard-
3200L is measured gas componant.Calculate 15 days final cumulative gas and methane phase yield.
1 straw methane fermentation result of table
Experimental group improves 61.29% than control group unit mass stalk gas production as can be seen from Table 1, unit mass straw
Stalk methane production improves 60.82%.It is obtained for by pretreated stalk gas production and methane production and significantly carries
It is high.
It can be seen that the advantage that experimental group is fermented after fermenting 7 days is embodied well, the drying straw of experimental group 57.14g
15 days methane phase total amounts of stalk fermentation are higher by 5201mL than control group..
Embodiment 7：
A kind of application of the pretreated straw that processing method of maize straw obtains in lactic acid is prepared：
Pretreated straw preparation method is the same as embodiment 6.
Lactic acid fermentation processes are as follows：Measuring the carbon-free MRS culture mediums of 100mL, (formula of carbon-free MRS culture mediums is：10g
Peptone, 10g beef extracts, 5g dusty yeasts, 1g Tween 80s, 2g K2HPO4, 5g sodium acetates, 2g lemon acid diamines,
0.2gMgSO4·7H2O, 0.05gMnSO4·H2O, distilled water are settled to 1000ml, pH 6.5-6.8), addition 3g is dried pre-
Handle stalk, 30U cellulases, by volume 1：(preserving number is 100 inoculation bacillus coagulans FL209 seed liquors
CCTCCNO：M2015773 30 DEG C of incubators) are placed in and carry out 48h lactic fermentations, in fermentation 12h, for 24 hours, 36h, 48h are sampled, and
With sodium hydroxide regulation system PH to 6.5-7.0.Lactic acid content is measured by HPLC methods.
2 stalk lactic fermentation result of table
Stalk can effective microbe conversion lactic acid after handling as can be seen from Table 2.Handle stalk lactic fermentation lactic acid producing amount
Increase with the increase of fermentation time, 0.451g is up in fermentation every gram of dry straw lactic acid yield of 48h.
Claims (8)
1. a kind of preprocess method of maize straw, includes the following steps：Maize straw adds Ca after pulverizing and sieving or blocking
(OH)2And water, maize straw and Ca (OH)2Mass ratio be 100：1-10；Final content of water in system 50-85%, 0-50 after mixing
DEG C sealing and standing 1-10 days, is passed through the gas containing carbon dioxide to stalk pH to 6.0-8.5.
2. according to the method described in claim 1, be crushed to after the maize straw naturally dry below 10 mesh or machinery cut
Break to below 5cm.
3. according to the method described in claim 1, maize straw and Ca (OH)2Mass ratio be 100：8, final content of water in system
For 75% (w/w).
4. according to the method described in claim 1, the temperature of sealing and standing is 25-30 DEG C, time of repose is 6-9 days.
5. according to the method described in claim 1, the gas containing carbon dioxide includes：The cigarette that various burners generate
The gas tail gas that either fermentation tank generates is passed directly into air.
6. application of the stalk that method described in claim 1 obtains in biogas is prepared.
7. application of the stalk that method described in claim 1 obtains in lactic acid is prepared.
8. application according to claim 7 is the nothing that drying stalk prepared by 3g is added to 100mL when preparing lactic acid
In the carbon-free MRS culture mediums of carbon MRS culture mediums, then fermented with bacillus coagulans FL209.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810176982.8A CN108148879A (en) | 2018-03-04 | 2018-03-04 | A kind of preprocess method of maize straw |
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| CN201810176982.8A CN108148879A (en) | 2018-03-04 | 2018-03-04 | A kind of preprocess method of maize straw |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110643638A (en) * | 2019-09-25 | 2020-01-03 | 中国农业大学 | Straw treatment method and application thereof |
| CN113016423A (en) * | 2021-03-02 | 2021-06-25 | 黑龙江省农业科学院 | Tomato cultivation greenhouse and quality-improving cultivation method of greenhouse tomatoes |
| CN114908133A (en) * | 2022-04-19 | 2022-08-16 | 天津商业大学 | Method for improving yield of reducing sugar by hydrolyzing corn straws through biological combined ammoniation pretreatment |
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| CN105586295A (en) * | 2016-01-22 | 2016-05-18 | 竹山县鑫源皂素有限责任公司 | Bacillus coagulans, culture method thereof and application of Bacillus coagulans in preparation of bio-organic fertilizer |
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| US20050250192A1 (en) * | 2004-03-04 | 2005-11-10 | Univeristy Of Florida Research Foundation, Inc. | Production of chemicals from lignocellulose, biomass or sugars |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110643638A (en) * | 2019-09-25 | 2020-01-03 | 中国农业大学 | Straw treatment method and application thereof |
| CN110643638B (en) * | 2019-09-25 | 2021-08-10 | 中国农业大学 | Straw treatment method and application thereof |
| CN113016423A (en) * | 2021-03-02 | 2021-06-25 | 黑龙江省农业科学院 | Tomato cultivation greenhouse and quality-improving cultivation method of greenhouse tomatoes |
| CN113016423B (en) * | 2021-03-02 | 2021-11-09 | 黑龙江省农业科学院 | Tomato cultivation greenhouse and quality-improving cultivation method of greenhouse tomatoes |
| CN114908133A (en) * | 2022-04-19 | 2022-08-16 | 天津商业大学 | Method for improving yield of reducing sugar by hydrolyzing corn straws through biological combined ammoniation pretreatment |
| CN114908133B (en) * | 2022-04-19 | 2024-02-13 | 天津商业大学 | Method for improving reducing sugar produced by corn straw hydrolysis through biological combined ammoniation pretreatment |
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