CN109793840A - Radix tetrastigme wound healing promoting cream and preparation method thereof - Google Patents
Radix tetrastigme wound healing promoting cream and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of radix tetrastigme wound healing promoting cream and preparation method, preparation method is to include the following steps: that radix tetrastigme root tuber crushes deionized water, gained lysate vacuum filter is added, and filtrate is concentrated to get Lemna paucicostata；Radix tetrastigme wound healing promoting cream is by following weight content at being grouped as: Lemna paucicostata 10%-15%, borneol 0.5%-2%, glycerin monostearate 10%-15%, stearic acid 15%-20%, glycerine 8%-12%, triethanolamine 2%-4%, ethylparaben 0.5%, surplus are deionized water；Mentioned component is mixed, radix tetrastigme wound healing promoting cream is obtained.The radix tetrastigme wound healing promoting cream can effectively inhibit the bacteria breed of wound circumference, thus reach anti-inflammatory, antibacterial, dispel swollen, putrefaction-removing granulation-promoting, wound healing the effect of, and it is easy to use.
Description
Technical field
The invention belongs to field of medical technology, and in particular to a kind of radix tetrastigme wound healing promoting cream and preparation method thereof.
Background technique
Wound healing refers to body by external force, and the tissues such as skin occur more answering process, packet after detachment or defect
The complex combination that the regeneration and granulation tissue hyperplasia, cicatricial tissue for including various tissues are formed, the collaboration for showing various processes are made
With.The basic process of the wound healing of wound: acute inflammation stage → hyperplasia phase → cicatrization phase → epidermis and other groups
Knit regeneration.It is using operation needle mostly because the patient of larger wound caused by body surface operation or wound is in healing process
Method carries out wound reparation, and existing stickup cream is easy to fall off or deform from wound site when user's carry out activity,
Wound is contacted with extraneous bacterium, and wound is caused to be difficult to heal.
Radix tetrastigme, scientific name tetratigma hemsleyanum, alias gold thread, which is hung, to be collapsed sound, the flat rattan of three leaves, has angle Japanese Cayratia Herb, is stone mouse, small flat
Rattan, stone monkey, soil are through ball, three leaves pair.Its root tuber is known as hemsley rockvine root, belongs to Angiospermae, Dicotyledoneae, rose algae Asia
Guiding principle, vitaceae.It is the exclusive valuable ingredient of traditional Chinese medicine in China, perennial herbaceous stem climbing vine, dioecism, nature and flavor slight bitter,
Channel tropism is flat, nontoxic, with dried root or all herbal medicine.Wild radix tetrastigme is mainly distributed on Jiangxi, Zhejiang, Fujian, Anhui, wide
West, part in Sichuan mountain area, mainly have kind of a kind: one is brown Chinese wistaria radix tetrastigme, the maturity period is close to the coat color of root rattan
Puce is grown in Jiangxi, Zhejiang mountainous area to the north of bright 37.3 ° of degree；Another kind is cyan rattan radix tetrastigme, and the surface color of rattan is
Bluish white is grown in some areas in 27.30 ° of Fujian in the south of China's degree of unraveling silk, Hunan, Sichuan, Guangdong, Guangxi, Yunnan.Chinese medicine
Material radix tetrastigme is distributed mainly on Hainan, Guangdong, Hong Kong, Guangxi, Yunnan, Guizhou, Sichuan, Chongqing, Hunan, Hubei, Jiangxi, good fortune
It builds, Zhejiang each province, which is suitble to be grown on more than mean annual precipitation is, the mean sea level Yunnan-Guizhou Plateau below, the Changjiang river
Middle and lower reaches Plain and southeast knob, this area are subtropical zone monsoon moist climate, the continental moist climate in temperate zone, temperature
Band monsoon moist climate, average temperature of the whole year ° or more, the sunshine-duration is long, and vegetation is in great numbers.
Radix tetrastigme has clearing heat and detoxicating, blood circulation promoting and dispersing pathogen accumulation, anti-inflammatory analgetic, dispelling pathogenic wind and eliminating phlegm, regulating qi-flowing for strengthening spleen and other effects.It is known as
" bouvardin " cures mainly high fever, tonsillitis, bronchitis, pneumonia, sphagitis, hepatitis, gastritis, cervicitis, lymph
Tuberculosis, septicemia and viral meningitis, external application venomous snake bite whole year can adopt, cleaning, drying, dry or using fresh herb.Radix tetrastigme
Efficacy effect, the successive dynasties, name doctor respectively had discussion, specific as follows:
Compendium of Material Medica records " tetratigma hemsleyanum, bitter in taste, pungent, cool, clearing heat and detoxicating, promoting blood circulation by removing wind ".
" the civil common herbal medicine in Zhejiang ": record " controls children's wind-heat, infantile convulsion and hernia pain；It controls snake to sting ".
" Illustrated Book on Plants " records " hemsley rockvine root can control infantile hyperpyrexia, stop abdominal pain, slurry be taken to take after mixing it with water ".
" Jiangxi herbal medicine " records " controlling children with high fever induced convulsions, pneumonia, hepatitis, traumatic hemorrhage ".
" Chinese draft " records " heat reliving and toxin-eliminating；Expelling wind and activating blood flow.Main hyperpyretic convulsion；Pneumonia；Asthma；Hepatitis；Ephritis；Rheumatism
Numbness pain；Traumatic injury；Carbuncle furunculosis furuncle；Eczema；Snakebite."
" promoting blood circulation to remove blood stasis strengthens the bone for " the practical Chinese herbal medicine in Guangxi newly selects " record.Control traumatic injury, lumbago due to wind-wetness evil, furuncle is wet
Rash."
" Illustrated Book on Plants " record " takes slurry to take after mixing it with water, controls abdominal pain in children, bring down a fever.Control traumatic injury, married woman's irregular menstruation,
Apply all nameless sores or boils."
Therefore radix tetrastigme is natural " bouvardin " that Western medicine can not replace, and has very high medical value and economy
Value.
The current existing method for preparing liquid adhesive bandage using radix tetrastigme has the following two kinds:
The invention " a kind of Chinese medicinal liquid band-aid and preparation method " of method one, 2012105738821, discloses in one kind
Medical fluid body adhesive bandage, contains following component and its weight fraction ratio: Lemna paucicostata 1-15, chitosan 1-10, ethyl alcohol 1-25,
Glycerol 0.5-2, hydrogen peroxide 0.5-3, menthol 0.5-5, purified water 1-30, pharmaceutical grade azone, the 0.02-2.0% of total amount.Have
It is easy to use, do not limited by wound and present position, after coating can fast filming, easy osmotic absorption, to human skin
Stimulate it is small, without allergic reaction and drug resistance；Promote musculature reparation and regeneration, protection and wound healing, shortens healing
Time, the problems such as effectivelying prevent wound infection；Its antibacterial and the main component of filmogen are degradable, easy organism-absorbing material
Material, biocompatibility is good, can voluntarily degrade, safety and environmental protection.
Lemna paucicostata is prepared using following methods:
1) it, is impregnated 3~5 minutes after the washing of radix tetrastigme root tuber with Eusol, it is clean then to drench Xian with drinking water, dries in the air
It is dry；
2), the radix tetrastigme root tuber crushing and beating that will be dried, slurries are put into microwave abstracting tank, and the purified water that equivalent is added is stirred
It mixes uniformly, opens the extraction of microwave abstracting tank and filter to obtain extract liquor in 3~5 minutes；
3) extract liquor, is heated to 38~42 DEG C, is handled 6~8 hours with alpha-amylase, digests the starch in solution completely
Solution after must digesting；
4) solution is atomized after eluent filtering with pressure type through macroporous resin adsorption with 50% ethanol elution after, digesting
Drier is dried up to three leafiness extract dry powder, and the control of atomization drying temperature is in 45-50 DEG C, atomizing pressure
5000kpa。
The invention " liquid bandage and preparation method thereof " of method two, 2017107360726 discloses a kind of liquid wound
Patch and preparation method thereof.The liquid bandage includes following components and its parts by weight: 20~60 parts of radix tetrastigme extracting solution；It is white
10~50 parts of splendid achnatherum extracting solution；5~50 parts of filmogen；20~200 parts of volatile solvent；0.5~15 part of plasticizer；Moisturizer
0.5~10 part；1~10 part of Laurocapram.Liquid bandage provided by the invention can be in the rapid shape of the surface of a wound after being coated on affected part
At one layer of soft, not easily to fall off, nontoxic nonirritant skin sparing film, there is hemostasis, prevent wound infection, waterproof etc.
Function.
The Effect of Alcohol Extracting Procedureto Total of radix tetrastigme extracting solution specifically: radix tetrastigme leaf be crushed into 20 meshes, be placed in 10 times it is anhydrous
Impregnate 12 hours in ethyl alcohol, then refluxing extraction is colourless to filtrate for several times under the conditions of 60 DEG C, extracts 2 hours every time, merging filtrate
And be concentrated, obtain radix tetrastigme extracting solution.
It joined ethyl alcohol in liquid adhesive bandage described in the above method one, for the patient for having wound, may have
Irritation pain, and poor air permeability.The shortcomings that liquid adhesive bandage described in method two equally exists poor air permeability.
For synthesis, after the painting of aforesaid liquid adhesive bandage is spread, can not rapid-curing cutback, and hinder the intercommunication with air, cause to hurt
Mouth does not reach air, is easy cutaneous necrosis；When wound is serious, liquid applicator is in great pain (especially to contain alcohol in formula
When)；When wound infection, the smell is awful for liquid adhesive bandage.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of radix tetrastigme wound healing promoting cream and preparation method thereof.
In order to solve the above technical problem, the present invention provides a kind of preparation methods of radix tetrastigme wound healing promoting cream, including
Following steps:
1), by radix tetrastigme root tuber crushed after being dried, deionized water is added in resulting root tuber powder, ultrasound makes root tuber powder
Last sufficiently dissolution, obtains lysate；
2), by lysate vacuum filter, gained filtrate is concentrated (being concentrated by Rotary Evaporators), obtains Lemna paucicostata
(for radix tetrastigme effective active composition extract)；
3), setting formula:
Radix tetrastigme wound healing promoting cream is by following weight content at being grouped as:
Lemna paucicostata 10%-15%, borneol 0.5%-2%, glycerin monostearate 10%-15%, stearic acid
15%-20%, glycerine 8%-12%, triethanolamine 2%-4%, ethylparaben 0.5%, surplus are deionized water；
4) glycerin monostearate, stearic acid, ethylparaben are uniformly mixed under heating condition, obtain solution I；
Borneol is added in solution I downwards in heat-retaining condition and is uniformly mixed, the solution I containing borneol is obtained；
First Lemna paucicostata, glycerine, deionized water are uniformly mixed under heating condition, then at heat preservation and stirring bar
It is uniformly mixed after triethanolamine is added under part, obtains solution II；
5), under heat preservation and stirring condition, the solution I containing borneol is added dropwise in solution II, time for adding is
30~60s；It is cooled to room temperature after being added dropwise, obtains radix tetrastigme wound healing promoting cream.
Another: resulting radix tetrastigme wound healing promoting cream is stored in a conventional manner, is filled.
The improvement of preparation method as radix tetrastigme wound healing promoting cream of the invention:
Solution I is to be prepared at 60 ± 5 DEG C of temperature (heating water bath)；Borneol is added under heat-retaining condition, must contain
There is the solution I of borneol；
Preparation temperature of the preparation temperature (heating water bath) of solution II with solution I；
Under heat-retaining condition and stirring condition, the solution I containing borneol is added dropwise to solution II.
The further improvement of preparation method as radix tetrastigme wound healing promoting cream of the invention, step 1) are as follows:
The sieve of 24 mesh will be crushed to after the drying to moisture content≤5% of radix tetrastigme root tuber；(preferably according to 1g/8~12ml
Deionized water is added, ultrasonic technological parameter are as follows: power in solid-liquid ratio 1g/10ml) in resulting root tuber powder (500g):
600±100w；Frequency: 45 ± 5KHz, time are 30 ± 5min.
The present invention is gone back while providing the radix tetrastigme wound healing promoting cream being prepared using the above method.
The composition proportion of radix tetrastigme wound healing promoting cream and the primary efficacy of every kind of ingredient are described in table 1 below.
The composition proportion of table 1, radix tetrastigme wound healing promoting cream
Radix tetrastigme wound healing promoting cream of the invention, main active are Lemna paucicostata, add borneol rush
It into anti-inflammatory, removes necrosis and promote granulation, under the cooperation of special process and specific auxiliary material, obtaining can effectively realize that the radix tetrastigme of wound healing promotees
Ointment for wound healing.The radix tetrastigme wound healing promoting cream can effectively inhibit the bacteria breed of wound circumference, to reach anti-inflammatory, resist
Bacterium, dispel swollen, putrefaction-removing granulation-promoting, wound healing the effect of, and it is easy to use.Raw material sources of the present invention are extensive, processing technology letter
Single, chemical agent content is few, without any side effects to human body, safely and effectively.
Radix tetrastigme wound healing promoting cream of the invention need to be set and save at shady and cool ventilation drying.
Radix tetrastigme wound healing promoting cream application method of the invention are as follows: external application after clean wound, takes appropriate this product uniformly to apply
It smears in wound (can uniformly fill entire wound surface), 3 times a day.
In the present invention,
1, radix tetrastigme active component detection
It is detected using effective active composition of the UPLC-MS method to radix tetrastigme
Chromatographic condition: Waters CORTECS C18(2.1mm×100mm,1.6μm)；Mobile phase: solvent A (acetonitrile) with it is molten
Agent B (0.1% formic acid water), gradient elution (0~0.5min, solvent A 8%~10%；0.5~2.5min, solvent A 10%~
15%；2.5~4.5min, solvent A 15%~20%；4.5~6.5min, solvent A 20%~40%；6.5~8.5min, it is molten
Agent A (40%~60%), flow velocity: 0.25mL/min, column temperature: 45 DEG C, sample volume: 1 μ L.
Mass Spectrometry Conditions: Negative electrospray ionization mode, capillary voltage 2.5kv, orifice potential 50v, desolventizing air-flow N2
800L/h, 200 DEG C of desolventizing temperature, taper hole air-flow N250L/h, 150 DEG C of ion source temperature, Extractor 3.00V, collision
Gases argon.
Test solution preparation: taking radix tetrastigme pulverizing medicinal materials, crosses 60 meshes and obtains radix tetrastigme medicinal powder.Take radix tetrastigme
0.5g, it is accurately weighed, it sets in triangular flask, 80% methanol 25mL, close plug is added in precision, and weighed weight is ultrasonically treated (power
250W, frequency 50kHz) 30min, it lets cool, then weighed weight, supplies less loss weight with 80% methanol, shake up, 0.22 μm of filter membrane filter
It crosses, takes subsequent filtrate, dilute 5 times, measured after internal standard is added by 1:1 (300:300 μ L).
Reference substance solution preparation: precision weighs neochlorogenic acid, Quercetin, Kaempferol, catechin, Kaempferol -3-0- rue
Glucosides, astragalin, resveratrol, isovitexin, rutin, each 1.0000mg of apiolin reference substance, are separately added into the pure methanol of 1mL
(chromatographic grade) dissolves it sufficiently and is diluted to stock solution, spare.
It is accurate respectively to measure test sample and each 1 μ L of reference substance solution, Ultra Performance Liquid Chromatography instrument is injected, from mass spectrum software
Obtain following result:
(1) neochlorogenic acid:
tRIt is m/z 353 [M-H] for quasi-molecular ion peak under 3.05min negative ion mode --, slough shape after-caffeoy
At fragments characteristic m/z 191 [M-H-caffeoy] -- fragment ion peaks are waited, are analysed according to Elemental Composition, the compound molecule formula
For C16H18O9.Speculate that the substance is neochlorogenic acid accordingly.
(2) Quercetin:
tRIt is m/z 301 [M-H]-for quasi-molecular ion peak under 10.38min negative ion mode, in conjunction with two H-shaped at m/z
The peak of 303 [M+H]-, is analysed according to Elemental Composition, which is C15H10O7.Speculate that the substance is Quercetin accordingly.
(3) Kaempferol:
tRBe m/z 287 [M+H] for quasi-molecular ion peak under 10.36min positive ion mode --, obtain a molecule H-shaped at
M/z 288 [M+2H] --, it is analysed according to Elemental Composition, which is C15H10O6, speculate that the substance is kaempferia galamga accordingly
Phenol.
(4) catechin:
tRIt is m/z 289 [M-H] for quasi-molecular ion peak under 4.30min negative ion mode --, lose a molecule CO2It generates
Fragments characteristic m/z 245 [M-H-CO2] --, in tRIt is 291 [M+ of m/z for quasi-molecular ion peak under 4.29min positive ion mode
H] --, 1,3 open loop of A ring cracks the peak to form fragments characteristic m/z 139, is analysed according to Elemental Composition, which is
C15H14O6, speculate that the substance is catechin accordingly.
(5) Kaempferol -3-0- rutinoside:
tRIt is m/z 593 [M-H] for quasi-molecular ion peak under 7.43min negative ion mode --, flavones part is lost, is generated
M/z285 includes the fragment of glycosyl part, is analysed according to Elemental Composition, which is C27H30O15, speculating accordingly should
Substance is Kaempferol -3-0- rutinoside.
(6) astragalin:
tRIt is m/z 447 [M-H] for quasi-molecular ion peak under 7.82min negative ion mode --, break to form fragments characteristic
The peak of m/z 285, then slough CH2O forms the fragment of m/z 255, and the fragment of m/z 255 sloughs a molecule glycosyl and forms m/z
227 fragments, are analysed according to Elemental Composition, which is C21H20O11, speculate that the substance is astragalin accordingly.
(7) white Chenopodiaceae family alcohol:
tRIt is m/z 227 [M-H] for quasi-molecular ion peak under 9.52min negative ion mode --, two molecule H-shaped are obtained into m/
Z 229 [M+H] --, tRIt is m/z 229 [M+H] for quasi-molecular ion peak under 9.55min positive ion mode --, lose-C6H6O shape
At fragments characteristic m/z 135 [M-H-C6H6O] --, a molecule H-shaped is obtained at m/z 230 [M+2H] --.According to Elemental Composition
Analysis, the compound molecule formula are C14H12O3, speculate that the substance is white Chenopodiaceae family alcohol accordingly.
(8) isovitexin:
tRIt is m/z 431 [M-H] for quasi-molecular ion peak under 6.65min negative ion mode --, a molecule H-shaped is obtained into m/
The peak of z 432, and fragments characteristic m/z 311 [M-H-C4H8O4] is formed by C ring crack solution --, which loses CO again and generates m/
The peak of z 283, tR are that quasi-molecular ion peak is m/z 433 [M+H] under 6.64min positive ion mode --, according to Elemental Composition
Analysis, the compound molecule formula are C21H20O10, speculate that the substance is isovitexin accordingly.
(9) rutin:
tRIt is m/z 609 [M-H]-for quasi-molecular ion peak under 6.57min negative ion mode, sloughs-Rha-Glc and obtain m/z
300 [M-H-Rha-Glc]-wait fragment ion peaks, are analysed according to Elemental Composition, which is C27H30O16, accordingly
Speculate that the substance is rutin.
(10) apiolin:
tRIt is m/z 271 [M+H]-for quasi-molecular ion peak under 8.08min positive ion mode, C ring is broken to obtain m/z
163 fragment, then the peak of m/z 434 is formed in conjunction with quasi-molecular ion, it is analysed according to Elemental Composition, which is
C15H10O5, speculate that the substance is apiolin accordingly.
The effect of above-mentioned 10 kinds of active constituents, is as described in Table 2.
Table 2, radix tetrastigme active constituent and its effect
From upper table 2 it is found that in radix tetrastigme effective active composition have the effects that antibacterial, it is anti-oxidant, remove necrosis and promote granulation, be formed into
The ideal natural origin of wound healing promoting cream.
Specific embodiment
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This:
Embodiment 1, a kind of preparation method of radix tetrastigme wound healing promoting cream, successively carry out following steps:
1) sieve of 24 mesh, will be crushed to after the drying to moisture content≤5% of radix tetrastigme root tuber；
According to the solid-liquid ratio of 1g/10ml, deionized water is added in resulting root tuber powder 500g and carries out ultrasound, power:
600w；Frequency: 45KHz, time 30min；To make root tuber powder sufficiently dissolve, lysate is obtained.
2), by lysate, (vacuum degree 98kPa) is filtered under vacuum condition, and gained filtrate passes through Rotary Evaporators (evaporation
Temperature setting is 80 DEG C) it is concentrated into and closely does, obtain Lemna paucicostata (for radix tetrastigme effective active composition extract)；
3), setting formula:
Radix tetrastigme wound healing promoting cream is by following weight content at being grouped as:
Lemna paucicostata 10%, borneol 0.5%, glycerin monostearate 10%, stearic acid 15%, glycerine 8%, three
Ethanol amine 2%, ethylparaben 0.5%, surplus are deionized water；
4), glycerin monostearate, stearic acid, ethylparaben are stirred evenly under 60 ± 5 DEG C of heating water bath mixed
It closes, obtains solution I；Borneol is added in solution I downwards in heat-retaining condition and stirs evenly mixing, obtains the solution I containing borneol；
First Lemna paucicostata, glycerine, deionized water are uniformly mixed under 60 ± 5 DEG C of heating water bath, then at guarantor
It is uniformly mixed after triethanolamine is added under the conditions of gentle agitation, obtains solution II；
5), under 60 ± 5 DEG C and stirring condition, the solution I containing borneol that temperature is 60 ± 5 DEG C, which is added dropwise to temperature, is
In 60 ± 5 DEG C of solution II, time for adding 30s；Rear cooled to room temperature is added dropwise, obtains radix tetrastigme wound healing promoting
Cream.
Another: resulting radix tetrastigme wound healing promoting cream is sealed after filling in a conventional manner, is protected from light storage.
2~embodiment of embodiment 7, the formula for changing the step radix tetrastigme wound healing promoting cream 3), remaining is equal to implementation
Example 1.Formula is described in table 3 below
The formula of table 3,1~embodiment of embodiment 7
Following radix tetrastigme pharmacology is carried out to the resulting radix tetrastigme wound healing promoting cream of 1~embodiment of above-described embodiment 7
It examines:
Test the bacteriostasis of one, radix tetrastigme wound healing promoting cream
After radix tetrastigme wound healing promoting cream is mixed with water according to 1:2 volume ratio, radix tetrastigme wound healing promoting cream dilution is obtained.
Take the uniform staphylococcus aureus bacterium solution of dilution, escherichia coli bacterium solution, pseudomonas aeruginosa bacterium solution, green pus bar
Bacterium bacterium solution, staphylococcus epidermis bacterium solution, enterococcus bacterium solution, using dilution spread flat band method respectively by staphylococcus aureus bacterium
Liquid, escherichia coli bacterium solution, pseudomonas aeruginosa bacterium solution, Pseudomonas aeruginosa bacterium solution, staphylococcus epidermis bacterium solution, enterococcus bacterium solution are equal
It is even to be inoculated on prepared solid medium that (bacterial concentration is 1 × 106Cfu/ml), after the completion of inoculation, culture medium is set
It is cultivated for 24 hours in 37 DEG C of constant incubator；
After taking-up, 10 μ L of radix tetrastigme wound healing promoting cream dilution is instilled into media surface, is placed again into 37 DEG C of perseverance
It is cultivated in warm incubator for 24 hours, takes out, there is inhibition zone to be formed.Antibacterial circle diameter size is measured, each parallel laboratory test 6 times is averaged
Value.Acquired results are described in table 4 below.
The fungistatic effect of table 4, radix tetrastigme wound healing promoting cream
Chinese medicine antibacterial susceptibility grade classification, referring to corresponding antibiotic standard, antibacterial circle diameter > 20mm is Gao Min, Cong Shangbiao
Experimental result is it is found that radix tetrastigme has certain fungistatic effect, and significant effect.
For further verify radix tetrastigme promotion wound healing effect, using mouse experiment.
Experiment two promotees more to test
90 mouse are randomly divided into 9 groups, are 1 blank control group (not making any medication processing), 1 matrix group respectively
(giving wound healing promoting cream base matter), 7 radix tetrastigme wound healing promoting cream groups (respectively corresponding 1~embodiment of embodiment 7).Abdominal cavity
Inject 5% chloraldurate 0.08mL (10g)- 1Anesthesia, after with shaver shave off back of mice hair, area about 3cm × 3cm,
Depilatory cream is then smeared, scrapes unhairing after acting on 5min, then with dipping in water cotton balls for shaving area wiped clean, then with the wiping of 75% ethyl alcohol
Disinfection is wiped, the round skin of diameter about 1.5cm is cut off with aseptic operation, wound is deep to fascia, with sterile yarn after the completion of operation
Cloth wrapping, next day start medication, and medicaments uniformity is filled entire wound surface with cotton swab every time.Observe Wound exudate amount
Number and extinction time, wound start heal (surface of a wound periphery starts furfur) and heal completely time (incrustation all falls off,
New skin is grown).And on day 3, the 6th day, the 9th day, wound area is recorded with hyaline membrane tracing method within the 12nd day, and count record wound and be cured
Product × 100% before conjunction rate (%)=(area after area-medication before medication)/medication.
The formula of the wound healing promoting cream base matter is borneol 2%, glycerin monostearate 15%, stearic acid 20%, third
Triol 12%, triethanolamine 4%, ethylparaben 0.5%, surplus are deionized water.
The wound healing rate of table 5, each experimental group
It can be obtained from upper table experimental result, radix tetrastigme has the apparent effect for promoting wound healing, effectively shortens wound and is cured
The time of conjunction, in the formula range that the present invention is set, under the premise of effective component is raised, wound healing effect is more preferably.It is real
Applying example 7 is best case.
Experiment three, the experiment of repair cell film
In cell film layer, oxidative pressure causes the peroxidating of phosphatide first, generates to cell membrane damage substance, radix tetrastigme
The noxious products efficiently can be reduced to nontoxic alcohols by effective active composition in extract, be made to play protection cell membrane
With, experiment specific steps are as follows:
1) compound concentration is each 10mL of peroxidating linoleic acid aqueous solution of 3mmol/mL, 6mmol/mL, 15mmol/mL；
2) compound concentration is calf serum (BSA) the aqueous solution 10mL of 30mmol/ml；
3) Lemna paucicostata aqueous solution (0.05%, 0.1%, 0.2%) 10mL of different content is configured；
4) configured solution is mixed in certain sequence, order by merging is successively that BSA- Lemna paucicostata-peroxide is sub-
Oleic acid；
5) reaction solution prepared is incubated for 3h at 37 DEG C；
6) reaction product is diluted (about 100-1000 times), is analyzed with HPLC method, and Lemna paucicostata energy is detected
It is no peroxide linoleic acid to be reduced to nontoxic alcohols, to play the role of protecting cell membrane.
Table 6, radix tetrastigme effective active composition are to the linoleic reduction rate of peroxidating
Note: reduction rate=(peroxide linoleic acid amount after peroxide linoleic acid amount-reaction before reacting)/peroxide linoleic acid amount before reacting
× 100%
As can be known from the above table, radix tetrastigme effective active composition has very high reduction rate for peroxidating linoleic acid, and effectively
Activity component concentration is bigger, and reduction rate is higher.Cell film layer peroxide can be reduced to nontoxic by radix tetrastigme effective active composition
Alcohols to play the role of protecting cell, and then has the effect of myogenic.
" Lemna paucicostata " in the formula of embodiment 7 is changed to " 2017107360726 invention " liquid by comparative example 1
Bandage and preparation method thereof " preparation radix tetrastigme extracting solution ", remaining is equal to embodiment 7.
Comparative example 2-1,7 step 1) ultrasonic technique parameter of embodiment is changed to conventional ultrasonic technique parameter, specifically: function
Rate 500w, frequency 20KHz, time 15min；Remaining is equal to embodiment 7.
Comparative example 2-2,7 step 2) vacuum filter of embodiment is changed to conventional filtering；Remaining is equal to embodiment 7.
Comparative example 2-3, the borneol in the formula of embodiment 7 is changed to menthol, content is constant；Remaining is equal to embodiment 7.
The step 4) of embodiment 7 and step 5) are changed to following content by comparative example 3: by the ingredient in step 3) formula
It is directly stirred under 60 ± 5 DEG C of water-bath, mixing time is 1 hour.Remaining is equal to embodiment 7.
" triethanolamine " in the formula of embodiment 7 is changed to " Arabic gum " by comparative example 4, and content is constant, remaining is equal to
Embodiment 7.
Above-mentioned all comparative examples are promoted more to test according to experiment two is described, acquired results are as shown in table 7 below.
The wound healing rate organized obtained by table 7, each comparative example
| Test group | 3 days (%) | 6 days (%) |
| Blank control group | 20 | 38 |
| Promoting healing cream base matter group | 35 | 53 |
| Comparative example 1 | 42 | 68 |
| Comparative example 2-1 | 40 | 65 |
| Comparative example 2-2 | 49 | 76 |
| Comparative example 2-3 | 43 | 68 |
| Comparative example 3 | 47 | 77 |
| Comparative example 4 | 48 | 78 |
The above list is only a few specific embodiments of the present invention for finally, it should also be noted that.Obviously, this hair
Bright to be not limited to above embodiments, acceptable there are many deformations.Those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (5)
1. the preparation method of radix tetrastigme wound healing promoting cream, it is characterized in that including the following steps:
1), by radix tetrastigme root tuber crushed after being dried, deionized water is added in resulting root tuber powder, ultrasound fills root tuber powder
Divide dissolution, obtains lysate；
2), by lysate vacuum filter, the concentration of gained filtrate obtains Lemna paucicostata；
3), setting formula:
Radix tetrastigme wound healing promoting cream is by following weight content at being grouped as:
Lemna paucicostata 10%-15%, borneol 0.5%-2%, glycerin monostearate 10%-15%, stearic acid 15%-
20%, glycerine 8%-12%, triethanolamine 2%-4%, ethylparaben 0.5%, surplus are deionized water；
4) glycerin monostearate, stearic acid, ethylparaben are uniformly mixed under heating condition, obtain solution I；Yu Bao
Borneol is added in solution I uniformly mixes downwards by warm condition, obtains the solution I containing borneol；
First Lemna paucicostata, glycerine, deionized water are uniformly mixed under heating condition, under heat preservation and stirring condition
It is uniformly mixed after triethanolamine is added, obtains solution II；
5), under heat preservation and stirring condition, the solution I containing borneol is added dropwise in solution II, time for adding be 30~
60s；It is cooled to room temperature after being added dropwise, obtains radix tetrastigme wound healing promoting cream.
2. the preparation method of radix tetrastigme wound healing promoting cream according to claim 1, it is characterized in that:
Solution I is to be prepared at a temperature of 60 ± 5 DEG C；Borneol is added under heat-retaining condition, obtains the solution I containing borneol；
Preparation temperature of the preparation temperature of solution II with solution I；
Under heat-retaining condition and stirring condition, the solution I containing borneol is added dropwise to solution II.
3. the preparation method of radix tetrastigme wound healing promoting cream according to claim 1 or 2, it is characterized in that the step 1)
Are as follows:
The sieve of 24 mesh will be crushed to after the drying to moisture content≤5% of radix tetrastigme root tuber；According to the solid-liquid ratio of 1g/8~12ml,
Deionized water, ultrasonic technological parameter are as follows: power: 600 ± 100w are added in resulting root tuber powder；Frequency: 45 ± 5KHz, when
Between be 30 ± 5min.
4. the preparation method of radix tetrastigme wound healing promoting cream according to claim 3, it is characterized in that the radix tetrastigme promotees wound
Mouthful healing ointment is by following weight content at being grouped as:
Lemna paucicostata 15%, borneol 2%, glycerin monostearate 15%, stearic acid 20%, glycerine 12%, three ethyl alcohol
Amine 4%, ethylparaben 0.5%, surplus are deionized water.
5. the radix tetrastigme wound healing promoting cream being prepared using such as Claims 1 to 4 either method.
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