CN105232603A - Folium artemisiae argyi extract preparation method - Google Patents
Folium artemisiae argyi extract preparation method Download PDFInfo
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Abstract
The invention relates to a folium artemisiae argyi extract preparation method which includes choosing dried folium artemisiae argyi with water content lower than 10%, soaking dried folium artemisiae argyi in isopropyl alcohol and ethylalcohol respectively to extract effective components of dried folium artemisiae argyi, mixing soak solution and then concentrating the soak solution under vacuum by twice in an external circulator, and finally reducing to obtain a folium artemisiae argyi extract. The preparation method has the advantages that utilization rate of folium artemisiae argyi is increased, extraction of related effective components is complete, extraction purity is high, and extraction efficiency is good; accordingly, the preparation method is practical and effective and is applicable to production in scale.
Description
Technical field
The present invention relates to a kind of preparation method of Chinese herbal medicine extract, particularly a kind of preparation method of Folium Artemisiae Argyi extract.
Background technology
Folium Artemisiae Argyi calls Folium Artemisiae Argyi, Du Aiye, wither Artemisia, and be the dried leaves of feverfew Chinese mugwort, be herbaceos perennial, be a kind of conventional Chinese herbal medicine, warm in nature, bitter in the mouth, has the effects such as dispersing cold for relieving pain, warming the meridian for stopping bleeding, anti-bacteria and anti-virus.Be distributed in the ground such as Heilungkiang, Jilin, Liaoning, Hebei, Shandong, Anhui, Jiangsu, Zhejiang, Guangdong, Guangxi, Jiangxi, Hunan, Hubei, Sichuan, Guizhou, Yunnan, Shaanxi, Gansu.Although Folium Artemisiae Argyi is widely distributed throughout the country, wild resource is horn of plenty comparatively, and its quality often affects by the place of production.In the Song dynasty, as well, so far, people generally believe that Folium Artemisiae Argyi is good with Qichun, Hubei product person to Song Yihou for the north Chinese mugwort that Folium Artemisiae Argyi produces with volt road, Tangyin, Henan and the Hai Ai that Ningbo of Zhejiang is produced.
Folium Artemisiae Argyi is as China's Chinese medicine, be widely used as oral medicine very early and be used for disease therapy, modern technologies are again for the extensive use of Folium Artemisiae Argyi opens more wide prospect, develop the multiple product such as Folium Artemisiae Argyi toothpaste, Folium Artemisiae Argyi bath agent, Oleum Folium Artemisiae Argyi essence, Radix Artemisia ordosicae pillow, health-care waist belt, Artemisia argyi Levl. et Van. var. argyi cv. Qiai mosquito incense, what developing in addition take Folium Artemisiae Argyi as the medicated cigarette of primary raw material, not containing nicotine, or can be used as the succedaneum of medicated cigarette.Clinically in order to treat few abdomen cold type of pain, coldness and unbalance in meridians, cold womb infertile, spit blood, epistaxis, metrorrhagia is through many, gestation hemorrhage, digestive tract tumor, pulmonary carcinoma, thyroid tumor, cancer of pancreas, external treatment skin pruritus.Although this medicinal plants commonly uses existing several thousand history among the people, the medicinal component of Radix Artemisia ordosicae is analysed scientifically and utilized, then starts from modern age.
Modern science research shows, the medicinal function of Folium Artemisiae Argyi derives from wherein contained chemical substance, and the chemical composition of Folium Artemisiae Argyi mainly contains volatile oil after deliberation, mainly contains 60 kinds of compositions such as 2-methyl butanol, 2-hexenoic aldehyde, tricyclene, α-thujene in volatile oil.Produce the volatile oil of wild Folium Artemisiae Argyi from Shandong Laoshan and identify 34 kinds of compositions, what wherein content was higher has limonene, α-absinthol, α-phellandrene and citronellol etc.32 compositions such as hydration lauro lene, Isosorbide-5-Nitrae-cineole, artemisia ketone, piperitone and widdrene are identified from the volatile oil of Herb.As a kind of extensive distribution and the Chinese crude drug be easy to get, the medical value of Folium Artemisiae Argyi can be develop and useedd further.
The chemical composition aspect of Folium Artemisiae Argyi finds that it is except containing except main component volatile oil, and also containing tannin, flavone, alcohol, polysaccharide, trace element and other organic principles etc., these elements effectively can be reconciled human body domestic demand and be asked.
And in pharmacological research, find that Folium Artemisiae Argyi has antiviral, relievings asthma, antitussive, eliminate the phlegm, antiallergic, hemostasis and the effect such as anticoagulation, enhancing immunologic function, all have inhibitory action to anthrax bacillus, α-hemolytic streptococcus, beta hemolytic streptococcus, diphtheria corynebacterium, Diplococcus pneumoniae, staphylococcus aureus, bacillus subtilis etc. in vitro.The volatile oil wherein extracted from Folium Artemisiae Argyi have relieving asthma, antitussive, expectorant, antibacterial, the effect such as antiallergic, hepatoprotective function of gallbladder promoting, facilitating digestion and complement activation; wherein principle active component is terpinenol; directly act on trachea; can to relieving asthma eliminating phlegm and relieving cough; adjustment maincenter; have obvious protective effect to drug-induced asthma, and can extend acetylcholine add histamine draw roar incubation period.The water logging agent of Folium Artemisiae Argyi has inhibitory action to Common Pathogenic dermatophytes.
Folium Artemisiae Argyi decocts use mainly with entering compound decoction in the use of Chinese medicine, be still the using method of most of doctor and hospital at present, but use still aobvious trouble, and Folium Artemisiae Argyi is after extracting the process of discarding the dross and selecting the essential, remain the original composition of medical material, decrease the trouble of decoction, do not affect effect of crude drug.
Folium Artemisiae Argyi extract is brown ceramic powder product, is with the Folium Artemisiae Argyi medical material of drying for raw material, the Folium Artemisiae Argyi extract powder obtained through a series of course of processing.The extraction ratio of usual Folium Artemisiae Argyi is about 20%, and namely the mass ratio of extract and medical material is 1:5, but in order to strengthen the utilization rate of medical material, so its extraction ratio need further raising, to avoid waste as far as possible.
Summary of the invention
For solving the problem, the invention discloses a kind of preparation method of Folium Artemisiae Argyi extract, this preparation method extraction efficiency is high, extract comparatively abundant, and the Folium Artemisiae Argyi extract purity obtained by the method is also higher.
Technical scheme of the present invention is: a kind of preparation method of Folium Artemisiae Argyi extract, comprises the following steps:
(1), choose moisture lower than 10% dry moxa leaf, be crushed to 50-200 object Folium Artemisiae Argyi powder, at room temperature soaked by organic solvent and filter for 15-30 hour, soak moves in storage tank for the first time, slag body soaks filtration in 15-30 hour again by organic solvent, and second time soak again moves in storage tank and merges with first time soak;
(2), the Folium Artemisiae Argyi soak in storage tank is carried out first time concentrating under reduced pressure by outer circulation concentrator, after concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.05-0.15 times of Folium Artemisiae Argyi soak volume, collects Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure;
(3), by the Folium Artemisiae Argyi of collection first time concentrated solution carry out second time concentrating under reduced pressure by outer circulation concentrator, receive cream after second time is concentrated and obtain Folium Artemisiae Argyi extract.
The organic solvent soaking Folium Artemisiae Argyi powder in described step (1) is isopropyl alcohol, and wherein the weight ratio of Folium Artemisiae Argyi powder and isopropyl alcohol is 1:(10-15); The organic solvent soaking slag body to be volumetric concentration be 70% ethanol, wherein slag body and ethanol weight ratio are 1:(5.0-6.0).Soaking Folium Artemisiae Argyi powder in currently available technology is generally be solvent with ethanol, and the isopropyl alcohol that the present invention uses especially significantly advantage, first its plant cell wall will be destroyed owing to extracting Folium Artemisiae Argyi effective ingredient, and isopropyl alcohol can lipid material quickly in dissolved cell wall as the ethanol of ratio of solvent same concentrations, cause intracellular matter stripping, and to volatile oil in Folium Artemisiae Argyi, the dissolving power of the lipophilicity substances such as pyrite is stronger than ethanol, also be less expensive solvent, energy and water freely mix, so use isopropyl alcohol can improve the extraction efficiency of effective ingredient in Folium Artemisiae Argyi significantly in the first step.And the slag body after being soaked first time uses ethanol to do to soak for the second time, the cellularity soaking rear Folium Artemisiae Argyi due to first time destroys, isopropyl alcohol has dissolved most chemical composition, but also have some residual components to fail to be dissolved in isopropyl alcohol, now isopropyl alcohol in slag body can be failed the abundant remaining extracts active ingredients extracted out by ethanol.
Aperture is adopted to be that the filter membrane of 5-10 μm filters when filtering in described step (1).In order to retain more effective ingredient, the aperture of filter membrane can not be too little, otherwise can not through some macromole, some materials in Folium Artemisiae Argyi such as polysaccharide are insoluble to alcohol solvent, usually polysaccharide has part to precipitate after with Ethanol Treatment, isopropyl alcohol then has good dissolubility to polysaccharide, thus this important constituent of polysaccharide is retained.Aperture is that the filter membrane porosity of 5-10 μm is high, resistance is little, filtering velocity is fast, adsorb minimum, the aperture of 5-10 μm through polysaccharide and other macromolecular compounds, can remove again the residue such as impurity, stalk in Folium Artemisiae Argyi, extraction of the present invention so also can be made more abundant.
In described step (2), the temperature of concentrating under reduced pressure is 60-65 DEG C for the first time, and the vacuum of concentrating under reduced pressure is higher than-0.08Mpa for the first time, and concentrating under reduced pressure time first time is 4-6 hour.Ethanol and isopropyl alcohol all have stronger volatility, wherein the boiling point of ethanol is 78.3 DEG C, the boiling point of isopropyl alcohol is 82.45 DEG C, BITAI is not high for temperature concentrated under reduced pressure atmosphere, it is too fierce that too high temperature can make solvent seethe with excitement, cause the bad control of process concentrated, and the lower composition of some Folium Artemisiae Argyi volatile oil mid-boiling points can be lost; On the other hand in order to the speed accelerating concentrating under reduced pressure is raised the efficiency, temperature is arranged also should not be too low.First time concentrating under reduced pressure vacuum higher than-0.08Mpa, concentrating under reduced pressure reduces the boiling point of solvent, and solvent can be evaporated rapidly, when vacuum is greater than-0.08Mpa, the boiling point of solvent close to 60 DEG C, therefore temperature is set to 60-65 DEG C the most suitable.Concentrated after 4-6 hour, just can obtain volume is Folium Artemisiae Argyi soak volume 0.05-0.15 concentrated solution doubly.
After described step (2) concentrating under reduced pressure of middle first time, Folium Artemisiae Argyi first time concentrated solution proportion under temperature is 60-65 DEG C of condition is 0.900-1.000.
In described step (3), the temperature of second time concentrating under reduced pressure is 60-65 DEG C, and the vacuum of second time concentrating under reduced pressure is higher than-0.08Mpa, and the second time concentrating under reduced pressure time is 2.5-4 hour.The composition in Folium Artemisiae Argyi soak is concentrated in together effectively because first time is concentrated, and volume is only the 0.05-0.15 of soak volume doubly, therefore the time that second time concentrates does not need oversize, need not more than 4 hours, but be preferably no less than 2.5 hours, such second time is concentrated can collect all the components as much as possible, and reduces concentrated solution volume, makes proportion reach requirement.Here temperature is identical with step (2) with the principle of the setting of vacuum.
In described step (3), after second time concentrating under reduced pressure, Folium Artemisiae Argyi second time concentrated solution proportion under temperature is 60 DEG C of-65 DEG C of conditions is 1.200-1.250.
Beneficial effect of the present invention: compared to common Folium Artemisiae Argyi extract preparation method; method of the present invention improves the utilization rate of medical material; extract more abundant; and extraction efficiency is high; the extract purity obtained is also relatively high, is a kind of practicability and effectiveness and is applicable to the preparation method of large-scale production.
Detailed description of the invention
Below in conjunction with detailed description of the invention, illustrate the present invention further, following detailed description of the invention should be understood and be only not used in for illustration of the present invention and limit the scope of the invention.
Embodiment 1
The preparation method of the Folium Artemisiae Argyi extract of the present embodiment, comprises the following steps:
(1), choose 100g moisture lower than 10% dry moxa leaf, 50 object Folium Artemisiae Argyi powders are crushed to pulverizer, at room temperature soak filtration in 15 hours by organic solvent, soak moves in storage tank for the first time, slag body soaks filtration in 15 hours again by organic solvent, and second time soak again moves in storage tank and merges with first time soak;
(2), by the Folium Artemisiae Argyi soak in storage tank carry out first time concentrating under reduced pressure by outer circulation concentrator, after concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.15 times of Folium Artemisiae Argyi soak volume, collects Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure;
(3), by the Folium Artemisiae Argyi of collection first time concentrated solution carry out second time concentrating under reduced pressure by outer circulation concentrator, receive cream after second time is concentrated and obtain Folium Artemisiae Argyi extract.
Embodiment 2
The preparation method of the Folium Artemisiae Argyi extract of the present embodiment, comprises the following steps:
(1), choose 100g moisture lower than 10% dry moxa leaf, 200 object Folium Artemisiae Argyi powders are crushed to pulverizer, at room temperature soak filtration in 20 hours by organic solvent, soak moves in storage tank for the first time, slag body soaks filtration in 18 hours again by organic solvent, and second time soak again moves in storage tank and merges with first time soak;
(2), by the Folium Artemisiae Argyi soak in storage tank carry out first time concentrating under reduced pressure by outer circulation concentrator, after concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.05 times of Folium Artemisiae Argyi soak volume, collects Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure;
(3), by the Folium Artemisiae Argyi of collection first time concentrated solution carry out second time concentrating under reduced pressure by outer circulation concentrator, receive cream after second time is concentrated and obtain Folium Artemisiae Argyi extract.
Embodiment 3
The preparation method of the Folium Artemisiae Argyi extract of the present embodiment, comprises the following steps:
(1), choose 100g moisture lower than 10% dry moxa leaf, 150 object Folium Artemisiae Argyi powders are crushed to pulverizer, at room temperature soak filtration in 24 hours by organic solvent, soak moves in storage tank for the first time, slag body soaks filtration in 25 hours again by organic solvent, and second time soak again moves in storage tank and merges with first time soak;
(2), by the Folium Artemisiae Argyi soak in storage tank carry out first time concentrating under reduced pressure by outer circulation concentrator, after concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.08 times of Folium Artemisiae Argyi soak volume, collects Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure;
(3), by the Folium Artemisiae Argyi of collection first time concentrated solution carry out second time concentrating under reduced pressure by outer circulation concentrator, receive cream after second time is concentrated and obtain Folium Artemisiae Argyi extract.
Embodiment 4
The preparation method of the Folium Artemisiae Argyi extract of the present embodiment, comprises the following steps:
(1), choose 100g moisture lower than 10% dry moxa leaf, 120 object Folium Artemisiae Argyi powders are crushed to pulverizer, at room temperature soak filtration in 28 hours by organic solvent, soak moves in storage tank for the first time, slag body soaks filtration in 26 hours again by organic solvent, and second time soak again moves in storage tank and merges with first time soak;
(2), by the Folium Artemisiae Argyi soak in storage tank carry out first time concentrating under reduced pressure by outer circulation concentrator, after concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.12 times of Folium Artemisiae Argyi soak volume, collects Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure;
(3), by the Folium Artemisiae Argyi of collection first time concentrated solution carry out second time concentrating under reduced pressure by outer circulation concentrator, receive cream after second time is concentrated and obtain Folium Artemisiae Argyi extract.
Embodiment 5
The preparation method of the Folium Artemisiae Argyi extract of the present embodiment, comprises the following steps:
(1), choose 100g moisture lower than 10% dry moxa leaf, 100 object Folium Artemisiae Argyi powders are crushed to pulverizer, at room temperature soak filtration in 30 hours by organic solvent, soak moves in storage tank for the first time, slag body soaks filtration in 30 hours again by organic solvent, and second time soak again moves in storage tank and merges with first time soak;
(2), by the Folium Artemisiae Argyi soak in storage tank carry out first time concentrating under reduced pressure by outer circulation concentrator, after concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.10 times of Folium Artemisiae Argyi soak volume, collects Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure;
(3), by the Folium Artemisiae Argyi of collection first time concentrated solution carry out second time concentrating under reduced pressure by outer circulation concentrator, receive cream after second time is concentrated and obtain Folium Artemisiae Argyi extract.
The difference of embodiment 6-10 and embodiment 1-5 is only that the organic solvent soaking Folium Artemisiae Argyi powder in step (1) is isopropyl alcohol, and wherein the weight ratio of Folium Artemisiae Argyi powder and isopropyl alcohol is followed successively by 1:10,1:11,1:12,1:13,1:15; The organic solvent soaking slag body to be volumetric concentration be 70% ethanol, wherein slag body and ethanol weight ratio are followed successively by as 1:5.0,1:5.2,1:5.5,1:5.7,1:6.0.
The difference of embodiment 11-15 and embodiment 6-10 is only to adopt when filtering in step (1) aperture to be that the filter membrane of 10 μm carries out filtering (aperture of filter membrane can also be other arbitrary values between 5,6,7,8,9 μm and 5-10 μm).
The difference of embodiment 16-20 and embodiment 6-10 is only the temperature of concentrating under reduced pressure for the first time in step (2) to be 60 DEG C, and (temperature can also be 61, 62, 63, 64, other arbitrary values between 65 DEG C and 60-65 DEG C), the vacuum of concentrating under reduced pressure is that (vacuum can also be-0.085 to-0.09Mpa for the first time,-0.088,-0.095,-0.10MPa and be greater than other arbitrary values of-0.08MPa), concentrating under reduced pressure time first time is that (concentrating under reduced pressure time first time can also be 4.1 in 4 hours, 4.2, 4.3, 4.5, 5.0, 5.6, 5.7, other random time values between 6.0 hours and 4-6 hour).
After the difference of embodiment 21-25 and embodiment 16-20 to be only in step (2) first time concentrating under reduced pressure Folium Artemisiae Argyi first time concentrated solution proportion under temperature is 60-65 DEG C of condition be 0.900 (proportion can also be 0.910,0.920,0.930,0.940,0.955,0.990,1.000 and 0.900-1.000 between other arbitrary values).
The difference of embodiment 26-30 and embodiment 6-10 is only that the temperature of second time concentrating under reduced pressure in step (3) is 60-65 DEG C, the vacuum of second time concentrating under reduced pressure be-0.09Mpa (vacuum can also for-0.085 ,-0.088 ,-0.095 ,-0.10MPa and be greater than other arbitrary values of-0.08MPa), the second time concentrating under reduced pressure time is 2.5 hours (the concentrating under reduced pressure time can also be other random time values between 2.6,2.7,2.8,2.9,3.0,3.5,4.0 hours and 2.5-4 hour for the second time).
The difference of embodiment 31-35 and embodiment 26-30 to be only in step (2) after second time concentrating under reduced pressure Folium Artemisiae Argyi second time concentrated solution proportion under temperature is 60-65 DEG C of condition be 1.200 (proportion can also be 1.210,1.220,1.230,1.240,1.250 and 1.200-1.250 between other arbitrary values).
Embodiment 36
The preparation method of the Folium Artemisiae Argyi extract of the present embodiment, comprises the following steps:
(1), choose 100g moisture lower than 10% dry moxa leaf, 100 object Folium Artemisiae Argyi powders are crushed to pulverizer, at room temperature soak filtration in 30 hours by the isopropyl alcohol of 1000g, soak moves in storage tank for the first time, slag body soaks filtration in 30 hours again by the ethanol of 70%, and second time soak again moves in storage tank and merges with first time soak; Adopt aperture to be that the filter membrane of 5 μm filters when wherein filtering, slag body and ethanol weight ratio are 1:5.0;
(2), by the Folium Artemisiae Argyi soak in storage tank by outer circulation concentrator, 60 DEG C, carry out first time concentrating under reduced pressure under the vacuum of-0.085MPa, the time is 4 hours; After concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.10 times of Folium Artemisiae Argyi soak volume, filter (filtering when concentrated solution exists by-product after first time is concentrated) with the filter membrane that aperture is 10 μm after concentrating under reduced pressure and collect Folium Artemisiae Argyi first time concentrated solution, the proportion of the concentrated solution obtained is 0.900;
(3), by the Folium Artemisiae Argyi first time concentrated solution collected by outer circulation concentrator, 60 DEG C, carry out second time concentrating under reduced pressure under the vacuum of-0.085MPa, the time is 2.5 hours; Concentrated solution proportion after second time is concentrated is 1.200, finally receives cream and obtains Folium Artemisiae Argyi extract.
Embodiment 37
The preparation method of the Folium Artemisiae Argyi extract of the present embodiment, comprises the following steps:
(1), choose 100g moisture lower than 10% dry moxa leaf, 100 object Folium Artemisiae Argyi powders are crushed to pulverizer, at room temperature soak filtration in 30 hours by the isopropyl alcohol of 1100g, soak moves in storage tank for the first time, slag body soaks filtration in 30 hours again by the ethanol of 70%, and second time soak again moves in storage tank and merges with first time soak; Adopt aperture to be that the filter membrane of 8 μm filters when wherein filtering, slag body and ethanol weight ratio are 1:5.2;
(2), by the Folium Artemisiae Argyi soak in storage tank by outer circulation concentrator, 63 DEG C, carry out first time concentrating under reduced pressure under the vacuum of-0.088MPa, the time is 4.5 hours; After concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.10 times of Folium Artemisiae Argyi soak volume, and collect Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure, the proportion of the concentrated solution obtained is 0.905;
(3), by the Folium Artemisiae Argyi first time concentrated solution collected by outer circulation concentrator, 63 DEG C, carry out second time concentrating under reduced pressure under the vacuum of-0.088MPa, the time is 3 hours; Concentrated solution proportion after second time is concentrated is 1.210, finally receives cream and obtains Folium Artemisiae Argyi extract.
Embodiment 38
The preparation method of the Folium Artemisiae Argyi extract of the present embodiment, comprises the following steps:
(1), choose 100g moisture lower than 10% dry moxa leaf, 100 object Folium Artemisiae Argyi powders are crushed to pulverizer, at room temperature soak filtration in 30 hours by the isopropyl alcohol of 1200g, soak moves in storage tank for the first time, slag body soaks filtration in 30 hours again by the ethanol of 70%, and second time soak again moves in storage tank and merges with first time soak; Adopt aperture to be that the filter membrane of 7 μm filters when wherein filtering, slag body and ethanol weight ratio are 1:5.4;
(2), by the Folium Artemisiae Argyi soak in storage tank by outer circulation concentrator, 62 DEG C, carry out first time concentrating under reduced pressure under the vacuum of-0.089MPa, the time is 5 hours; After concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.10 times of Folium Artemisiae Argyi soak volume, and collect Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure, the proportion of the concentrated solution obtained is 0.923;
(3), by the Folium Artemisiae Argyi first time concentrated solution collected by outer circulation concentrator, 62 DEG C, carry out second time concentrating under reduced pressure under the vacuum of-0.089MPa, the time is 3.4 hours; Concentrated solution proportion after second time is concentrated is 1.205, finally receives cream and obtains Folium Artemisiae Argyi extract.
Embodiment 39
The preparation method of the Folium Artemisiae Argyi extract of the present embodiment, comprises the following steps:
(1), choose 100g moisture lower than 10% dry moxa leaf, 100 object Folium Artemisiae Argyi powders are crushed to pulverizer, at room temperature soak filtration in 30 hours by the isopropyl alcohol of 1350g, soak moves in storage tank for the first time, slag body soaks filtration in 30 hours again by the ethanol of 70%, and second time soak again moves in storage tank and merges with first time soak; Adopt aperture to be that the filter membrane of 6 μm filters when wherein filtering, slag body and ethanol weight ratio are 1:5.8;
(2), by the Folium Artemisiae Argyi soak in storage tank by outer circulation concentrator, 65 DEG C, carry out first time concentrating under reduced pressure under the vacuum of-0.090MPa, the time is 5.5 hours; After concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.10 times of Folium Artemisiae Argyi soak volume, and collect Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure, the proportion of the concentrated solution obtained is 1.000;
(3), by the Folium Artemisiae Argyi first time concentrated solution collected by outer circulation concentrator, 65 DEG C, carry out second time concentrating under reduced pressure under the vacuum of-0.090MPa, the time is 3.5 hours; Concentrated solution proportion after second time is concentrated is 1.240, finally receives cream and obtains Folium Artemisiae Argyi extract.
Embodiment 40
The preparation method of the Folium Artemisiae Argyi extract of the present embodiment, comprises the following steps:
(1), choose 100g moisture lower than 10% dry moxa leaf, 100 object Folium Artemisiae Argyi powders are crushed to pulverizer, at room temperature soak filtration in 30 hours by the isopropyl alcohol of 1500g, soak moves in storage tank for the first time, slag body soaks filtration in 30 hours again by the ethanol of 70%, and second time soak again moves in storage tank and merges with first time soak; Adopt aperture to be that the filter membrane of 10 μm filters when wherein filtering, slag body and ethanol weight ratio are 1:6.0;
(2), by the Folium Artemisiae Argyi soak in storage tank by outer circulation concentrator, 64 DEG C, carry out first time concentrating under reduced pressure under the vacuum of-0.095MPa, the time is 6 hours; After concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.10 times of Folium Artemisiae Argyi soak volume, and collect Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure, the proportion of the concentrated solution obtained is 0.900;
(3), by the Folium Artemisiae Argyi first time concentrated solution collected by outer circulation concentrator, 64 DEG C, carry out second time concentrating under reduced pressure under the vacuum of-0.095MPa, the time is 4 hours; Concentrated solution proportion after second time is concentrated is 1.250, finally receives cream and obtains Folium Artemisiae Argyi extract.
Comparative example
Take 100g moisture lower than 10% dry moxa leaf, 100 object Folium Artemisiae Argyi powders are crushed to pulverizer, add the ethanol that 1200g volumetric concentration is 70%, divide three soak extraction, extraction time is respectively 30h, 24h, 12h, merge extractive liquid, carry out sucking filtration, discard slag body, gained filtrate is concentrated into 0.025 of former ethanol contend, finally receives cream and obtains Folium Artemisiae Argyi extract.
The Folium Artemisiae Argyi extract that embodiment 5,10,15,20,25,30,35,36,40 and comparative example obtain is carried out precise respectively, and the qualitative data claimed is listed in table 1.
The Folium Artemisiae Argyi extract that embodiment 5,10,15,20,25,30,35,36,40 and comparative example obtain is added 500ml distilled water respectively fully dissolve, then steam distillation heating extraction is carried out, volatile oil volume (ml) calculated yield (%) extracted according to every Ke Aiye, yield value is listed in table 1;
The Folium Artemisiae Argyi extract that embodiment 5,10,15,20,25,30,35,36,40 and comparative example obtain is added 2000ml distilled water respectively fully dissolve, take rutin as the content that object of reference measures total flavones in extract, ultraviolet-uisible spectrophotometer is used to measure absorbance, obtain concentration, calculate general flavone content (%), content value is listed in table 1;
The Folium Artemisiae Argyi extract that embodiment 5,10,15,20,25,30,35,36,40 and comparative example obtain is obtained polysaccharide precipitation respectively by alcohol deposition method, carries out precise after being dried, and calculate its mass fraction in extract, data are listed in table 1.
From table 1 data, the extractive content obtained by Folium Artemisiae Argyi extract preparation method of the present invention is apparently higher than the preparation method of comparative example, no matter be volatile oil, pyrite or polysaccharide all extract more abundant, purity is also higher, has good extraction efficiency.
Table 1
In view of the present invention program's embodiment is numerous, each embodiment experimental data is huge numerous, be not suitable for particularize herein to illustrate, but the content of the required checking of each embodiment is all close with the final conclusion obtained, so do not illustrate one by one the checking content of each embodiment herein.
The technical scope midrange non-limit part that this place embodiment is protected application claims and in embodiment technical scheme to the new technical scheme that the equal replacement of single or multiple technical characteristic is formed, equally all in the scope of protection of present invention; Simultaneously in all embodiments enumerated or do not enumerate of the present invention program, parameters in the same embodiment only represents an example (i.e. a kind of feasible scheme) of its technical scheme, and between parameters, there is not strict cooperation and qualified relation, wherein each parameter can be replaced, except special declaration mutually when stating ask without prejudice to axiom and the present invention.
Technological means disclosed in the present invention program is not limited only to the technological means disclosed in above-mentioned technological means, also comprises the technical scheme be made up of above technical characteristic combination in any.The above is the specific embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.
Claims (7)
1. a preparation method for Folium Artemisiae Argyi extract, is characterized in that, the method comprises the following steps:
(1), choose moisture lower than 10% dry moxa leaf, be crushed to 50-200 object Folium Artemisiae Argyi powder, at room temperature soaked by organic solvent and filter for 15-30 hour, soak moves in storage tank for the first time, slag body soaks filtration in 15-30 hour again by organic solvent, and second time soak again moves in storage tank and merges with first time soak;
(2), the Folium Artemisiae Argyi soak in storage tank is carried out first time concentrating under reduced pressure by outer circulation concentrator, after concentrating under reduced pressure, the volume of Folium Artemisiae Argyi first time concentrated solution is 0.05-0.15 times of Folium Artemisiae Argyi soak volume, collects Folium Artemisiae Argyi first time concentrated solution after concentrating under reduced pressure;
(3), by the Folium Artemisiae Argyi of collection first time concentrated solution carry out second time concentrating under reduced pressure by outer circulation concentrator, receive cream after second time is concentrated and obtain Folium Artemisiae Argyi extract.
2. the preparation method of Folium Artemisiae Argyi extract according to claim 1, is characterized in that: the organic solvent soaking Folium Artemisiae Argyi powder in step (1) is isopropyl alcohol, and wherein the weight ratio of Folium Artemisiae Argyi powder and isopropyl alcohol is 1:(10-15); The organic solvent soaking slag body to be volumetric concentration be 70% ethanol, wherein slag body and ethanol weight ratio are 1:(5.0-6.0).
3. the preparation method of Folium Artemisiae Argyi extract according to claim 1 and 2, is characterized in that: adopt aperture to be that the filter membrane of 5-10 μm filters when filtering in step (1).
4. the preparation method of Folium Artemisiae Argyi extract according to claim 1, it is characterized in that: in step (2), the temperature of concentrating under reduced pressure is 60-65 DEG C for the first time, the vacuum of concentrating under reduced pressure is higher than-0.08Mpa for the first time, and concentrating under reduced pressure time first time is 4-6 hour.
5. the preparation method of the Folium Artemisiae Argyi extract according to claim 1 or 4, is characterized in that: after step (2) concentrating under reduced pressure of middle first time, Folium Artemisiae Argyi first time concentrated solution proportion under temperature is 60-65 DEG C of condition is 0.900-1.000.
6. the preparation method of Folium Artemisiae Argyi extract according to claim 1, it is characterized in that: in step (3), the temperature of second time concentrating under reduced pressure is 60-65 DEG C, the vacuum of second time concentrating under reduced pressure is higher than-0.08Mpa, and the second time concentrating under reduced pressure time is 2.5-4 hour.
7. the preparation method of the Folium Artemisiae Argyi extract according to claim 1 or 6, is characterized in that: in step (3), after second time concentrating under reduced pressure, Folium Artemisiae Argyi second time concentrated solution proportion under temperature is 60 DEG C of-65 DEG C of conditions is 1.200-1.250.
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| CN111329756A (en) * | 2020-04-30 | 2020-06-26 | 江西源生狼和药业有限公司 | A method for extracting moxibustion rod containing folium Artemisiae Argyi |
| CN111603497A (en) * | 2020-06-28 | 2020-09-01 | 河北中医学院 | Folium artemisiae argyi extract and application thereof in preparing hypoglycemic and lipid-lowering medicines |
| CN117160412A (en) * | 2023-10-08 | 2023-12-05 | 宁波绿之健药业有限公司 | Preparation method and application of modified activated carbon for mugwort leaf extraction |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111329756A (en) * | 2020-04-30 | 2020-06-26 | 江西源生狼和药业有限公司 | A method for extracting moxibustion rod containing folium Artemisiae Argyi |
| CN111603497A (en) * | 2020-06-28 | 2020-09-01 | 河北中医学院 | Folium artemisiae argyi extract and application thereof in preparing hypoglycemic and lipid-lowering medicines |
| CN117160412A (en) * | 2023-10-08 | 2023-12-05 | 宁波绿之健药业有限公司 | Preparation method and application of modified activated carbon for mugwort leaf extraction |
| CN117160412B (en) * | 2023-10-08 | 2024-02-27 | 宁波绿之健药业有限公司 | Preparation method and application of modified activated carbon for mugwort leaf extraction |
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