CN109762744A - Fungal bacterial strain and its cultural method and purposes for degradable polyurethane plastics - Google Patents

Fungal bacterial strain and its cultural method and purposes for degradable polyurethane plastics Download PDF

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Publication number
CN109762744A
CN109762744A CN201910156110.XA CN201910156110A CN109762744A CN 109762744 A CN109762744 A CN 109762744A CN 201910156110 A CN201910156110 A CN 201910156110A CN 109762744 A CN109762744 A CN 109762744A
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bacterial strain
fungal bacterial
flavus
culture
agar
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Inventor
赛隆·可汗
许建初
桂恒
赛迪亚·娜迪尔
彼得·莫蒂默
叶磊
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Kunming Institute of Botany of CAS
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Kunming Institute of Botany of CAS
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Priority to CN201910156110.XA priority Critical patent/CN109762744A/en
Priority to CN202010879237.7A priority patent/CN111944704B/en
Priority to PCT/CN2019/080949 priority patent/WO2020177175A1/en
Publication of CN109762744A publication Critical patent/CN109762744A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/67—Aspergillus flavus
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2375/00—Characterised by the use of polyureas or polyurethanes; Derivatives of such polymers
    • C08J2375/04—Polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Abstract

The present invention provides one plant of fungal bacterial strain A.flavus G10 for being used for degradable polyurethane plastics, and microbial preservation number is: GDMCC 60537.Also provide a kind of method for cultivating fungal bacterial strain A.flavus G10, comprising: isolate fungal bacterial strain from the enteron aisle of cricket；Using PU as sole carbon source, cultivated the fungal bacterial strain to obtain culture solution in liquid medium；The culture solution is diluted and is coated on cured nutrient agar and the potato dextrose agar containing tetracycline antibiotic and obtains culture growth-gen；The culture growth-gen is subjected to secondary culture on fresh plate at 30 DEG C, until obtaining single fungal bacterial strain on each plate.Above-mentioned fungal bacterial strain A.flavus G10 degradable polyurethane plastics speed is fast.

Description

Fungal bacterial strain and its cultural method and purposes for degradable polyurethane plastics
Technical field
The present invention relates to biodegradable field, in particular to a kind of fungal bacterial strain for degradable polyurethane plastics, bacterial strain Culture and application thereof.
Background technique
Plastics are a kind of multifunctional materials, may be used with nearly the every aspect of our daily lifes.Global synthetic plastic Consumption per head is about annual 38 kilograms.From nineteen fifty by 2015, about 91,500,000 tons of plastics are produced, have been caused in the earth Surface generates about 69,450,000 tons of plastic wastes (Gyer etc., 2015).Very small part can recycle in the plastic wastes of generation (9%) and (12%) is burned, about 79% plastics assemble (Gyer still in refuse landfill, other earth's surfaces and marine environment Deng 2015).It is estimated that the urban solid garbage total amount produced every year is 2.430 hundred million tons, and waste plastics accounts for 13% in the U.S. (EPA report 2009, the U.S.).In Europe, waste plastic accounts for the 15-25% (Eurostat.2008) of 2.2 hundred million solid refuses.? China, waste plastics account for 12% (" China Statistical Yearbook ", 2001-2007) of domestic waste total amount.
Most of plastic garbage in refuse landfill is polyurethane (PU).PU is a kind of synthetic plastic, for manufacturing work The various objects of industry, automobile and medical field.PU accounts for 7% or so of global plastics total output, and global annual output is about 12,000,000 Ton, major part eventually become plastic garbage (Plastic Europe).Since its degradation in the environment can be ignored, Discarded PU plastic wastes are accumulated in soil, deposit and refuse landfill with ever-increasing speed, therefore are in environment The main reason for plastic pollution.The increasingly depleted and subsequent land pollution of PU plastics shortage degradability and refuse landfill Researcher is promoted to develop environmental-friendly biodegradable plastics, including biodegradable PU plastics.However, these can Time needed for the destiny of degradative plastics in the environment and its permineralization is not fully understood.In addition, waste plastic product Main methods include: refuse landfill landfill, burn and recycle, but all lasting without solving the environment of PU waste Property and its potential pollution problem (Macromolecules symposium, 2006；Waste Management, 2009).
On solving the problems, such as plastic wastes, biodegrade is simpler, endangers smaller.Current verified several microorganism energy Enough biodegradable plastics (Kaplan etc., 1979；Otake etc., 1995；Nakamiya etc., 1997；Cacciari etc., 1993； Sivan etc., 2008；Arkatkar etc., 2009；Atiq etc., 2010；Zafar etc., 2013,2014；Yang etc., 2014；Khan Deng 2017).Researcher has found the microorganism of degradation PU from soil, refuse landfill and compost；Wherein fungi quilt It is the discovery that dominant (Boubendir 1993；Crabbe etc., 1994；Nakajima-Kambe 1995；Baumgartner Deng 1997；Blake and Howard 1998；Akutsu etc., 1998；Howard and Blake 1999；Allen et al., 1999； Manna and Paul, 2000；Yamada-Onodera et al., 2001；Zafar et al., 2013,2014；Khan et al., 2017；). Fungi known secretes different types of hydrolase, including esterase, urase and protease (Khan etc., 2017).Research finds polyester Type PU is easier to be hydrolyzed (Tokiwa etc., 2009) by fungi than polyether-type PU.Biodegradable degree also with PU polymer and The chemical property of fungal species and change.Some fungal species need to add some chemical inducers, induce biodegradable institute The secretion of the enzyme needed.By by Curvularia senegalensis, Fusarium solani, Aureobasidium The microorganism consortium of pullulans and Cladosporium sp, composition carry out biodegrade to colloid polyester PU.Wherein, C.senegalensis is more effective, and it generates extracellular polyurethane enzyme (PUase) enzyme (Crabbe etc., 1994).
However, being that plastic degradation speed is slow using the main problem of plastic degradation microorganism.For example, from sludge, Polluted Soil The mixed culture of several bacteriums and fungal bacterial strain isolated in earth and marine sediment shows that biodegradation rate is extremely slow (Nadnda and Sahu, 2010).In addition, being dropped using the mixed microorganism culture separated from sludge, soil, muck, rubbish In the test for solving polystyrene, using isotope tracer measurement, its degradation 0.55% needs 11 weeks (Kaplan etc., 1979).True After being incubated for 3 months in the liquid culture of bacterium mould (Penicillium simplicissimum), observe that polyester (PE) has Slowly degradation (Yamada-Onodera etc., 2001).Recently, microorganism consortium terephthalate is reported The biological degradation rate of ester (PET) plastics is daily 0.13mg/cm-2(Yoshida etc., 2016).PU is divided due to its polymer property Son amount is high, and structure is complicated, is generally difficult to biodegradable in the natural environment.
In spite of the report about PU degrading microorganism, but so far it has been reported that degradation rate it is slower.It is so far Only, not yet it is found to have the method for the biodegradable PU with high-speed (such as in less than 30 days) and large-scale production.Therefore, It needs to develop more effective PU biodegrading process and improved biosystem, degradation PU rate is improved, so that this method can be in quotient Be able in industry using.
Summary of the invention
The invention mainly solves the technical problem of providing a kind of degradation speeds to be used for degradable polyurethane plastics faster Fungal bacterial strain, strain culture and application thereof.
One plant of fungal bacterial strain A.flavus G10 for degradable polyurethane plastics, microbial preservation number is: GDMCC 60537。
A kind of purposes of above-mentioned fungal bacterial strain in polyurethane plastics.
The polyurethane plastics is foam type or transparent type in one of the embodiments,.
One kind containing fungal bacterial strain A.flavus G10 bioreactor.
A kind of strain culture for cultivating above-mentioned fungal bacterial strain A.flavus G10.
A method of cultivating above-mentioned fungal bacterial strain A.flavus G10, comprising:
Fungal bacterial strain is isolated from the enteron aisle of cricket；
Using PU as sole carbon source, cultivated the fungal bacterial strain to obtain culture solution in liquid medium；
The culture solution is diluted and is coated on cured nutrient agar and Ma Ling containing tetracycline antibiotic Culture growth-gen is obtained on potato glucose agar medium；
The culture growth-gen is subjected to secondary culture on fresh plate at 30 DEG C, is obtained until on each plate Obtain single fungal bacterial strain.
The fluid nutrient medium is by the way that KH is added in deionized water in one of the embodiments,2PO4、K2HPO4、 MgSO4·7H2O、NH4NO3、NaCl、FeSO4·7H2And ZnSO O,4·7H2O is made.
The potato dextrose agar is by the way that horse is added in deionized water in one of the embodiments, Bell sweet potato starch, sugar and agar are made.
The nutrient agar is by the way that peptone, ox are added in deionized water in one of the embodiments, Digested tankage, NaCl and agar are made.
The fluid nutrient medium, nutrient agar and potato dextrose agar training in one of the embodiments, It supports base and is using preceding equal high-temperature sterilization.
Above-mentioned fungal bacterial strain can effectively in a short time degrade polyurethane plastics.When PU film and fungal bacterial strain When A.flavus G10 is cultivated together, fungal bacterial strain A.flavus G10 can effectively degrade PU piece, weekly loss of weight 4.4%.
Detailed description of the invention
Fig. 1 is upgrowth situation schematic diagram of the fungal bacterial strain of an embodiment on culture medium flat plate；
Fig. 2 is the mycelium and spore shape figure for the fungal bacterial strain that the present invention isolates, and wherein a is under optical microscopy Mycelia；B is conidium under optical microscopy；C, d, e are the conidium under SEM；
Attached drawing 3 is the schematic diagram of normal PU film (a) and G10 treated PU film (b)；
Attached drawing 4 is PU film SEM figure, and wherein a is normal PU film SEM figure, and b, c, d, e, f is that the processed PU of G10 is thin Film SEM figure；
Attached drawing 5 is using three-dimensional (3D) shape appearance figure of the PU film surface of atomic force microscope (AFM), and wherein A- expression is not located The 3D surface scan figure of the PU film of reason.B, C and D indicate the 3D surface scan figure that 2,4 and 6 hours PU films are handled with G10.
Specific embodiment
The present invention provides the fungal bacterial strain A.flavus G10 for degradable polyurethane plastics of an embodiment, micro- life Object deposit number is: GDMCC 60537.Collection: Guangdong Province's Culture Collection.
Above-mentioned fungal bacterial strain can be applied in degradable polyurethane plastics.
Specifically, polyurethane plastics is foam type or transparent type.
Specifically, polyurethane plastics is poly- [4,4- di-2-ethylhexylphosphine oxides (phenyl isocyanate)-salt -1,4-butanediol/bis- (third Glycol)/polycaprolactone-acetone].Specifically, chemical formula are as follows: poly- [4,4- di-2-ethylhexylphosphine oxides (phenyl isocyanate)-salt-Isosorbide-5-Nitrae-fourth Glycol/bis- (propylene glycol)/polycaprolactone-acetone] PU, bead be purchased from Aldrich Chemical Company, Inc.(beauty State)).According to the method for Khan et al., these beads are used to prepare PU film.In short, passing through the rotational oscillation in 150rpm Shake 30min in 250ml flask in device, by the PU pearl of about 1.25g be dissolved in 100ml tetrahydrofuran (PanreacQuimica, SA in).PU solution is poured into four glass culture dish, and by being at room temperature maintained at the big plastic casing with gel beads Solidify within 48 hours around PU diaphragm plate.After evaporating solvent, dry PU film is separated from culture dish, and stores at room temperature.
A kind of bioreactor containing fungal bacterial strain A.flavus G10, can be used for the PU that degrades.Bioreactor allows Environmental condition is controlled to provide the optimum growh of fungi.Specifically, which may include providing fungal bacterial strain and making Inoculum is generated with the bacterial strain.Inoculum can be used for being inoculated with it is small-sized containing petroleum-based plastics and minimal medium, it is medium-sized or Macro-organism reactor.It can monitor and control interior environmental conditions and output and input stream.Specifically, in an embodiment In, inside bioreactor environmental condition includes temperature, and pH, carbon source and mixing speed are controllable.Specifically, in an embodiment In, bioreactor can be the bioreactor or fed-batch bioreactor of continuous feed.Specifically, in an embodiment party In formula, bioreactor can be equipped with the recycling of fluent material.Specifically, in one embodiment, when plastics are biological anti- When answering in device, biodegrade can be monitored by sampling and monitoring.
Specific embodiment
It please refers to Fig. 1-5 and understands embodiment.
Embodiment 1: fungal bacterial strain A.flavus G10 separation screening:
S110, fungal bacterial strain is isolated from the enteron aisle of cricket.
Specifically, which can be cricket " Gryllus bimaculatus ".Specifically, divide from the enteron aisle of cricket Separate out fungal bacterial strain.
S120, using PU as sole carbon source, cultivated the fungal bacterial strain to obtain culture solution in liquid medium；
Specifically, by the way that each section of cricket enteron aisle is dissolved in sterile saline solution and incubates enteral in liquid In culture medium.
Wherein, fluid nutrient medium the preparation method comprises the following steps: in 1000ml deionized water add 0.7g KH2PO4、7.7g K2HPO4、0.7g MgSO4·7H20、1.0gNH4NO3、0.005g NaCl、0.002g FeSO4·7H2O、0.002g ZnSO4· 7H2O,0.001gxx.Fluid nutrient medium is sterilized using preceding in the completion in 15 minutes of 121 DEG C of high pressure sterilizations.
S130, the culture solution containing fungal bacterial strain is diluted and is coated on the cured nutrition fine jade containing tetracycline antibiotic Culture growth-gen is obtained on rouge culture medium and potato dextrose agar.
Specifically, 5.0g/L potato starch, 20.0g/L sugar and 15g agar are added in 1000mL deionized water and are made Standby potato dextrose agar (PDA) culture medium can specifically configure in Erlenmeyer flask.Specifically, it is distributing Before into culture dish, high pressure sterilization 20 minutes is completed to sterilize at 121 DEG C.
Specifically, by 10g peptone, 3g powdered beef, 5g NaCl and 15g agar is added in 1000mL deionized water and makes Standby nutrient agar.Specifically, before being assigned in culture dish, high pressure sterilization 20 minutes is completed to go out at 121 DEG C Bacterium.
S140, culture growth-gen is subjected to secondary culture on fresh plate at 30 DEG C, until on each plate Obtain single fungal bacterial strain.
Embodiment 2: the identification of fungi
Under room temperature (25-28 DEG C), the DNA of degradative fungi bacterial strain is separated, after purification at m alt extract agar (MEA) On plate.Morphological characterization is carried out by optical microscopy and stereo electron microscope.By molecular conformation and systematic growth point The PU degradative fungi being separated by decantation to, is identified as aspergillus flavus G10.
Specifically, identification method are as follows: Morphological characterization is carried out by optical microscopy and stereo electron microscope.In inside Transcribed spacer (ITS5/ITS4), large subunit (LR0R/LR5), the second large subunit of rna plymerase ii (fRPB2-5f/fRPB2- 7cR), calmodulin (CAL-228F/CAL2Rd) and β-carry out Molecular Identification.Tubulin (T1/T2) serial method.In the research The ITS sequence data of middle generation GenBank RiboaptDB (www http: // Blast.ncbi.nlm.nih.gov/ blast search is carried out in) to determine their most probable closely related taxons.Make With MAFFT v.7.215 (Katoh and Standley 2016:(http: //mafft.cbrc.jp/alignment/server/ Index.html single-gene sequence alignment) is carried out, and is compiled manually in BioEdit vv7.2.5 (Hall 2004) if necessary Volume.The Phylogenetic Analysis of correlation data is based on maximum likelihood (ML).With FigTree v1.4.0 program (Rambaut 2012) Show Phylograms, and in Microsoft powerpoint (2007) andCS5 (version 15.0.0, Adobe) in recombinated.San Jose, CA).
Embodiment 3: using the method for fungal bacterial strain A.flavus G10 degradable polyurethane plastics:
Condition of culture: being maintained at 24 ± 2 DEG C of temperature in the condition of incubator, relative humidity 75 ± 2%, light application ratio 8:10 item Under part, 300 crickets are cultivated, a total of 6 small incubators in an incubator there are 50 crickets in each incubator.
Wherein, control group: being 24 ± 2 DEG C in temperature, and relative humidity is 75 ± 2%, and light application ratio is the collating condition of 8:10 150 healthy adult crickets of lower raising feed and contain wheat bran, and the ratio of wheat embryo and yeast powder is respectively 10:3:1.In addition it mends Fill 0.2% vitamin powder and the total amount of water.
Experimental group: another group of 150 healthy adult crickets are 24 ± 2 DEG C in temperature, and relative humidity is 75 ± 2%, illumination It is raised under the collating condition that time is 8:10, feeding contains diet of 4 grams of PUF as sole carbon source.
By checking the weight of cricket, the weight of each cricket and the percentage of excrement are degraded to verify the PU of cricket.Often Three repetitions of a experiment, we determined that average value and standard error SE (n=3) (experimental group and control group) per treatment.It is logical The weight of five crickets of random measurement is crossed to determine the variation of biomass.After the zero-time (experiment starting point) and at interval of three days The excrement for measuring cricket, continues 18 days.Biodegradable degradation is checked by the appearance and weight loss of PU film.
In order to study the chemical digestion of the edible PU of cricket, excrement is analyzed by scanning electron microscope (SEM).? After three days, by the collecting dung of cricket in the sampling bag of sterilizing, continue 18 days, and is stored in -20 DEG C until further making With.The excrement of collection is dissolved in sterile water, is separated, wash and is separately stored in the small pieces PUF being discharged in excrement for SEM Analysis.
Determine that the surface of PU piece is opened up using atomic force microscope (AFM) (AFM, Dimension Icon, Veeco, USA) It flutters and cross-sectional, top view.By A.flavus G10 mycelia, mycelium and spore mortar and pestle are crushed, and it is thin to be coated in three pieces PU On film, cultivated at 37 DEG C 2,4 and 6 hours.PU piece is gently washed with sterile water and ethyl alcohol and is stored at room temperature for AFM points Analysis.Degradation is confirmed using two methods of AFM and SEM respectively.AFM is set as 2000nm and scans 3-d photographs.Scanning electricity Sub- microscope (SEM), is studied, acceleration voltage 3-7KV, amplification factor 50X- using ZEISS Sigma 300 4500X, resolution ratio are 200 μm of -200nm.Use AT-FTIR research and application chemical degradation.Spectral region is 4000 to 600cm-1, Resolution ratio is 4cm-1.Sample is placed on the ATR spot of the other part ATR and is slowly suppressed.Each processing and control sample Three be recycled and reused for analyzing.The roughness on the surface PU as the result is shown AFM, cavity, hole etc..By using SEM microscope, we It can be seen that growing fungi on PU film surface and forming hole on the surface.
Through experimental analysis it is found that in experimental group, fungal bacterial strain A.flavus G10 of the invention can degrade PU in 28 days. PU mass is unchanged in control group.
According to above-mentioned experiment condition, 5 groups are done simultaneously with 5 films, first group terminates when first week and reacts, and second Group terminates reaction when second week ... and so on, and the 5th group terminates reaction when the 5th week, as a result such as the following table 1 It is shown.It is demonstrated experimentally that fungal bacterial strain A.flavus G10 can when PU film is cultivated together with fungal bacterial strain A.flavus G10 With PU piece of effectively degrading, after stablizing, loss of weight 4.4% weekly.
PU loss of weight monitoring data in 1 experimental group of table
Time Initial weight (g) Final weight (g) Weight difference (g) Loss of weight percentage (g)
0th 0.349 0.0349 0.00 0.00%
First week 0.341 0.338 0.003 0.8%
Second week 0.347 0.342 0.005 1.4%
Third week 0.374 0.366 0.008 2.2%
4th week 0.340 0.325 0.015 4.4%
In addition, another it was found that, if PU film first passes through fungi A.flavus G10 pretreatment, biology drop Solution increases.Experiment discovery, as FeSO of the PU piece 1%45 points are radiated with being incubated for 24 hours in NaCl salting liquid and being exposed to UV Zhong Shi, weight reduce weekly 6.1%.
The above is only embodiments of the present invention, are not intended to limit the scope of the invention, all to utilize the present invention Equivalent structure or equivalent flow shift made by specification and accompanying drawing content is applied directly or indirectly in other relevant technologies Field is included within the scope of the present invention.

Claims (10)

1. one plant of fungal bacterial strain A.flavus G10 for degradable polyurethane plastics, microbial preservation number is GDMCC 60537。
2. purposes of the fungal bacterial strain as described in claim 1 in polyurethane plastics.
3. purposes according to claim 2, which is characterized in that the polyurethane plastics is foam type or transparent type.
4. one kind contains fungal bacterial strain A.flavus G10 bioreactor described in claim 1.
5. a kind of strain culture for cultivating fungal bacterial strain A.flavus G10 described in claim 1.
6. a kind of method for cultivating fungal bacterial strain A.flavus G10 described in claim 1 characterized by comprising
Fungal bacterial strain is isolated from the enteron aisle of cricket；
Using PU as sole carbon source, cultivated the fungal bacterial strain to obtain culture solution in liquid medium；
The culture solution is diluted and is coated on the cured nutrient agar containing tetracycline antibiotic and potato Portugal Culture growth-gen is obtained on grape sugar agar medium；
The culture growth-gen is subjected to secondary culture on fresh plate at 30 DEG C, it is single until being obtained on each plate A fungal bacterial strain.
7. the method for fungal bacterial strain A.flavus G10 according to claim 6, which is characterized in that the fluid nutrient medium For by the way that KH is added in deionized water2PO4、K2HPO4、MgSO4·7H2O、NH4NO3、NaCl、FeSO4·7H2And ZnSO O,4· 7H2O is made.
8. the method for fungal bacterial strain A.flavus G10 according to claim 6, which is characterized in that the potato grape Sugared agar medium by the way that potato starch, sugar and agar are added in deionized water by being made.
9. the method for fungal bacterial strain A.flavus G10 according to claim 6, which is characterized in that the nutrient agar training Base is supported by being made by the way that peptone, powdered beef, NaCl and agar are added in deionized water.
10. the method for fungal bacterial strain A.flavus G10 according to claim 6, which is characterized in that the Liquid Culture Base, nutrient agar and potato dextrose agar are using preceding equal high-temperature sterilization.
CN201910156110.XA 2019-03-01 2019-03-01 Fungal bacterial strain and its cultural method and purposes for degradable polyurethane plastics Withdrawn CN109762744A (en)

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CN202010879237.7A CN111944704B (en) 2019-03-01 2019-03-01 Fungus strain for degrading polyurethane plastics, and culture method and application thereof
PCT/CN2019/080949 WO2020177175A1 (en) 2019-03-01 2019-04-02 Fungal strain for degrading polyurethane plastic, culture method therefor and use thereof

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CN111671013A (en) * 2020-05-19 2020-09-18 华南农业大学 Edible micro-plastic remover and application thereof
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CN112481137B (en) * 2020-12-04 2022-08-19 南京工业大学 Cladosporium and application thereof in degradation of polyurethane plastic
CN112522358B (en) * 2020-12-04 2023-05-26 南京工业大学 System for evaluating polyurethane degradation
CN112553108A (en) * 2020-12-08 2021-03-26 南京工业大学 Bacillus brevis and application thereof in degradation of polyurethane
CN112553108B (en) * 2020-12-08 2023-01-24 南京工业大学 Bacillus brevis and application thereof in degradation of polyurethane
CN114410485A (en) * 2022-02-18 2022-04-29 中国科学院昆明植物研究所 Fungus strain for degrading polyurethane in soil and application thereof

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