CN109746129B - Integrated centrifugal machine - Google Patents
Integrated centrifugal machine Download PDFInfo
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- CN109746129B CN109746129B CN201711071241.5A CN201711071241A CN109746129B CN 109746129 B CN109746129 B CN 109746129B CN 201711071241 A CN201711071241 A CN 201711071241A CN 109746129 B CN109746129 B CN 109746129B
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Abstract
The invention discloses an integrated centrifuge, aiming at integrating the mixing, centrifugation and chemical reaction in the dairy product treatment into one device, shortening the pretreatment time of samples, improving the pretreatment efficiency of dairy products to be detected and enhancing the sensitivity and detection flux of chemical pollutants in the dairy products determined by the existing colloidal gold immunoassay products. The integrated centrifuge comprises a composite stirrer, a rotor and a motor, and is a device for integrally realizing the steps of uniformly mixing, centrifuging, chemical reaction and the like; the composite stirrer is a device with the functions of top cover and stirring; the rotor is an inverted cone-shaped structure and is a device for containing liquid and realizing various chemical reactions and physical separation; the motor is a device for providing power for centrifugation, and can enable the rotor to realize uniform mixing, centrifugation, drug release and chemical reaction at different rotating speeds. The integrated centrifuge provided by the invention can be used for processing materials such as dairy products, blood, tissues and the like; can be used for sample pretreatment in various fields such as clinical diagnosis, inspection and quarantine, food safety detection, judicial appraisal, drug detection and the like, and has wide market prospect.
Description
Technical Field
The invention relates to a chemical reaction device, in particular to a centrifuge which integrates the operations of blending, centrifugation and chemical reaction, and belongs to the technical field of biomedicine.
Background
Sample handling is essential in various assay testing practices. Especially for samples with complex matrixes, different processing methods are set for different detection instruments. The steps of mixing and centrifuging are the most common operations with certain chemical reactions. Wherein, the uniform mixing is mainly carried out on vortex and oscillation equipment so as to realize the uniform mixing of different components or carry out specific reaction; the centrifugation mainly depends on centrifuges of different models and matched rotors to realize the separation of different components; the chemical reaction is mainly carried out by various special devices.
Take the example of detecting chemical contaminants in dairy products by a colloidal gold detection card. Most of the traditional detection methods are direct sample loading, so that the simplicity of operation is realized, but the complex matrix of the dairy product causes the detection sensitivity to be obviously limited. After the milk sample to be detected is treated by uniform mixing, centrifugation and certain chemical reaction, the sensitivity of the detection card can be obviously improved compared with that of direct sample loading. But the complexity of the pretreatment obviously reduces the detection flux and easily causes the pollution of the sample to be detected. Simple and rapid sample pretreatment has important value for improving the flux and sensitivity of detection methods including colloidal gold detection.
Disclosure of Invention
Aiming at the defects in the milk product colloidal gold detection practice, the invention discloses an integrated centrifuge, aiming at integrating the uniform mixing, centrifugation and chemical reaction in the milk product treatment into one device, reducing the pretreatment time of samples, improving the pretreatment efficiency of milk products to be detected and enhancing the sensitivity and detection flux of chemical pollutants in the milk products measured by the existing colloidal gold immunodetection products. In order to achieve the purpose, the invention adopts the following technical scheme:
the integrated centrifuge comprises a composite stirrer, a rotor and a motor, and is a device for integrally realizing the steps of uniformly mixing, centrifuging, chemical reaction and the like; the composite stirrer is a device with the functions of top cover and stirring; the rotor is an inverted cone-shaped structure and is a device for containing liquid and realizing various chemical reactions and physical separation; the motor is a device for providing power for centrifugation, and can enable the rotor to realize uniform mixing, centrifugation, drug release and chemical reaction at different rotating speeds. Furthermore, the reverse taper rotor internally comprises a clamping groove, a barrier and a platform. The inverted cone design aims to automatically concentrate liquid subjected to chemical reaction or physical separation at a platform by utilizing gravity; the clamping groove is used for placing a reaction reagent wrapped by the capsule, the capsule in the clamping groove is dissolved by liquid in the rotor under the condition of a certain rotating speed, and the reaction reagent is released for chemical reaction; the barrier is perpendicular to the outer wall of the rotor and arranged centripetally, and aims to ensure that liquid enters the interior of the barrier in the acceleration process of the rotor and quickly accelerates along with the rotor to improve the centrifugal force, so that the centrifugal effect is effectively realized; the platform provides a place for the composite stirrer to stir the reaction solution and is used for collecting the liquid part after centrifugal separation; when the motor provides different rotating speeds, the dissolution and release of the capsule reagent in the clamping groove and the separation of the solution under the assistance of the barrier can be realized, and the chemical reaction and the collection of the centrifugal solution assisted by stirring on the platform can be realized.
The material of the integrated centrifuge can be plastic, and can also be all plastic materials such as metal, paper, glass and the like.
The sample pretreatment provided by the invention is matched with an integrated centrifuge, and can be used for processing materials such as dairy products, blood, tissues and the like; can be used for sample pretreatment in various fields such as clinical diagnosis, inspection and quarantine, food safety detection, judicial appraisal, drug detection and the like, and has wide market prospect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings obtained from the drawings belong to the protection scope of the present invention for those skilled in the art without creative efforts.
FIG. 1 is a combined view of an integrated centrifuge according to the present invention
In the drawings, the materials represented by the various reference numerals are listed below:
1. compounding a stirrer; 2. a rotor; 3. a motor;
FIG. 2 is a diagram of an integrated centrifuge according to the present invention
In the drawings, the materials represented by the various reference numerals are listed below:
1. compounding a stirrer; 2. a rotor; 3. a motor;
FIG. 3 is a bottom structure view of an integrated centrifuge rotor according to the present invention
In the drawings, the materials represented by the various reference numerals are listed below:
4. a card slot; 5. a barrier; 6. a platform;
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 application of an integrated centrifuge to colloidal gold assay detection after protein precipitation of dairy products
The method comprises the following operation steps: adding a rotor into the dairy product and adding a reaction reagent A; placing a capsule reagent B into the rotor clamping groove; starting a motor power supply; fourthly, after the rotor is covered with the composite stirrer, uniformly mixing the reagent A and the dairy product to be detected by the composite stirrer at the rotating speed of 1 (low speed, about 600rpm) to generate a first-step chemical reaction to generate protein precipitate; converting to a rotating speed of 2 (high speed, about 3000rpm), and rapidly realizing the aggregation and separation of denatured protein at the bottom of the barrier under the assistance of the barrier; sixthly, converting the speed to 3 (medium speed, about 1500rpm), dissolving and releasing the capsule reagent B in the clamping groove, and carrying out a second step of chemical reaction; seventhly, repeating the step 5, and separating the newly generated protein precipitate; stopping the centrifuge, and determining the target solution gathered by the collection platform under the action of gravity, such as detecting chemical pollutants in the dairy product by a colloidal gold detection card.
The integrated centrifuge provided by the embodiment of the invention is described in detail above, can effectively reduce the influence of protein in dairy products on chromatographic detection, improves the detection sensitivity, and is suitable for field matching use. The integrated centrifuge provided by the invention can be used for rapid sample pretreatment in the fields of clinical diagnosis, inspection and quarantine, food safety detection, judicial identification, medicine, drug detection and the like.
The principles and embodiments of the present invention have been described herein using specific examples, which are provided only to help understand the method and the core concept of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (2)
1. An integrated centrifuge is characterized in that the integrated centrifuge comprises a composite stirrer, a rotor and a motor, and is a device for integrally realizing the steps of uniformly mixing, centrifuging and chemical reaction; the composite stirrer is arranged at the upper part of the rotor and connected with the rotor, the motor is arranged at the lower part of the rotor, and the composite stirrer is a device with the functions of a top cover and stirring; the rotor is an inverted cone-shaped structure and is a device for containing liquid and realizing various chemical reactions and physical separation; the motor is a device for providing power for centrifugation, and can enable the rotor to realize uniform mixing, centrifugation, drug release and chemical reaction at different rotating speeds; the integrated centrifuge is used for the treatment of dairy products, blood and tissue materials or the pretreatment of clinical diagnosis, inspection and quarantine, food safety detection, judicial identification and drug detection samples; the rotor is internally provided with a clamping groove, a barrier and a platform, and the clamping groove, the barrier and the platform are arranged on the rotor; the inverted cone structure aims to automatically collect liquid subjected to chemical reaction or physical separation at a platform by utilizing gravity; the clamping groove is used for placing a reaction reagent wrapped by the capsule, and the liquid in the rotor dissolves the capsule in the clamping groove and releases the reaction reagent to carry out chemical reaction under the condition of a certain rotating speed; the barrier is vertical to the outer wall of the rotor and arranged centripetally; the platform provides a place for the composite stirrer to stir the reaction solution and is used for collecting the liquid part after centrifugal separation; when the motor provides different rotating speeds, the dissolution and release of a capsule reagent in the clamping groove can be realized, the separation of solution under the assistance of the barrier can be realized, and the stirring-assisted chemical reaction and the collection of centrifugal solution on the platform can be realized; the clamping groove is arranged between the platform and the outer wall of the rotor.
2. An integrated centrifuge as defined in claim 1 wherein: the material of the integrated centrifuge is all plastic materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711071241.5A CN109746129B (en) | 2017-11-03 | 2017-11-03 | Integrated centrifugal machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711071241.5A CN109746129B (en) | 2017-11-03 | 2017-11-03 | Integrated centrifugal machine |
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| Publication Number | Publication Date |
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| CN109746129A CN109746129A (en) | 2019-05-14 |
| CN109746129B true CN109746129B (en) | 2022-01-04 |
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| CN201711071241.5A Active CN109746129B (en) | 2017-11-03 | 2017-11-03 | Integrated centrifugal machine |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0106536A2 (en) * | 1982-09-15 | 1984-04-25 | Ortho Diagnostic Systems Inc. | Carousel microparticle separating, washing and reading device and method of use |
| CN202305303U (en) * | 2011-06-16 | 2012-07-04 | 丹东市百特仪器有限公司 | Circulation sample injection system of powder particle size testing |
| CN104316384A (en) * | 2014-09-30 | 2015-01-28 | 深圳市理邦精密仪器股份有限公司 | Centrifugal type quick gas-liquid mixing device and method applying same |
| WO2015145646A1 (en) * | 2014-03-27 | 2015-10-01 | 東京濾器株式会社 | Oil separator |
| CN107138293A (en) * | 2017-06-30 | 2017-09-08 | 吴素贞 | A kind of teaching experiment centrifuge |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE536671C2 (en) * | 2012-04-23 | 2014-05-13 | 3Nine Ab | Tapered disc elements for a rotor for centrifugal separators and rotors containing such disc elements |
-
2017
- 2017-11-03 CN CN201711071241.5A patent/CN109746129B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0106536A2 (en) * | 1982-09-15 | 1984-04-25 | Ortho Diagnostic Systems Inc. | Carousel microparticle separating, washing and reading device and method of use |
| CN202305303U (en) * | 2011-06-16 | 2012-07-04 | 丹东市百特仪器有限公司 | Circulation sample injection system of powder particle size testing |
| WO2015145646A1 (en) * | 2014-03-27 | 2015-10-01 | 東京濾器株式会社 | Oil separator |
| CN104316384A (en) * | 2014-09-30 | 2015-01-28 | 深圳市理邦精密仪器股份有限公司 | Centrifugal type quick gas-liquid mixing device and method applying same |
| CN107138293A (en) * | 2017-06-30 | 2017-09-08 | 吴素贞 | A kind of teaching experiment centrifuge |
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| CN109746129A (en) | 2019-05-14 |
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