CN109735611A - 一种用于检测骨髓衰竭综合征的基因组合、引物库、构建高通量测序文库的方法及其应用 - Google Patents
一种用于检测骨髓衰竭综合征的基因组合、引物库、构建高通量测序文库的方法及其应用 Download PDFInfo
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Abstract
Description
组分 | 反应体系(20μL) |
高保真PCR缓冲液 | 4 |
5倍浓度的引物库 | 4 |
DNA,10ng | Y |
去离子水 | (12-Y) |
总体积 | 20 |
温度 | 时间 |
50℃ | 10min |
55℃ | 10min |
60℃ | 20min |
10℃ | 保持(最多1小时) |
组分 | 体积 |
Switch溶液 | 4μL |
稀释特异性标签混合液 | 2μL |
DNA连接酶 | 2μL |
总体积(包含22μL消化的扩增子) | 30μL |
温度 | 时间 |
22℃ | 30min |
72℃ | 10min |
10℃ | 保持(最多1小时) |
样本编号 | Q>20数据量 | 测序reads数 | 靶标区域覆盖度 | 测序深度(x) | 均一性 |
样本1 | 583,540 | 654,588 | 97% | 624 | 91% |
样本2 | 719,580 | 785,456 | 94% | 746 | 94% |
Claims (10)
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Cited By (2)
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CN110331446A (zh) * | 2019-08-27 | 2019-10-15 | 上海鹍远生物技术有限公司 | Dna甲基化标志物筛查试剂盒及方法 |
CN111073961A (zh) * | 2019-12-20 | 2020-04-28 | 苏州赛美科基因科技有限公司 | 一种基因稀有突变的高通量检测方法 |
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CN103993096A (zh) * | 2014-06-09 | 2014-08-20 | 中国医学科学院血液病医院(血液学研究所) | 一种用于诊断先天性骨髓衰竭性疾病的试剂盒 |
CN104095878A (zh) * | 2005-11-09 | 2014-10-15 | 阿特西斯公司 | 多潜能成体祖细胞的免疫调节特性及其用途 |
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CN106350589A (zh) * | 2016-08-31 | 2017-01-25 | 汪道文 | 一种检测遗传性血管疾病致病基因的dna文库及其应用 |
CN107916290A (zh) * | 2017-12-29 | 2018-04-17 | 天津协和华美医学诊断技术有限公司 | 一种检测遗传性血小板减少症相关基因群的检测试剂盒 |
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2018
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CN104095878A (zh) * | 2005-11-09 | 2014-10-15 | 阿特西斯公司 | 多潜能成体祖细胞的免疫调节特性及其用途 |
CN103993096A (zh) * | 2014-06-09 | 2014-08-20 | 中国医学科学院血液病医院(血液学研究所) | 一种用于诊断先天性骨髓衰竭性疾病的试剂盒 |
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CN106350589A (zh) * | 2016-08-31 | 2017-01-25 | 汪道文 | 一种检测遗传性血管疾病致病基因的dna文库及其应用 |
CN107916290A (zh) * | 2017-12-29 | 2018-04-17 | 天津协和华美医学诊断技术有限公司 | 一种检测遗传性血小板减少症相关基因群的检测试剂盒 |
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CN110331446A (zh) * | 2019-08-27 | 2019-10-15 | 上海鹍远生物技术有限公司 | Dna甲基化标志物筛查试剂盒及方法 |
CN111073961A (zh) * | 2019-12-20 | 2020-04-28 | 苏州赛美科基因科技有限公司 | 一种基因稀有突变的高通量检测方法 |
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