CN1097195A - The synthesis technique of Antai growth promotor - Google Patents

Abstract

The synthesis technique of Antai growth promotor is made up of operations such as acidifying dextran, complexing, refining, preparation, sterilizations, and the dextran molecular-weight average of employing is 4000～9000, and the complexing time is 1～2 hour.Synthesis technique of the present invention is simple, and cost is low, the Antai growth promotor injection of making, the effect that has prevention and treatment piglet and other livestock hypoferric anemia, enhancing body immunizing power and promote to grow.

Description

The synthesis technique of Antai growth promotor

The present invention relates to a kind of veterinary drug, particularly treat the synthesis technique of the medicament of livestock hypoferric anemia.

Iron is the necessary a kind of trace element of animal body, and the storage of pig embryo iron is few, and the iron reserves are only 40～53 milligrams in the newborn piglet body, and the piglet early growth is fast, and the iron level of sow breast is low, and piglet is not if mend iron, and then the oxyphorase in the blood drops to ill threshold value very soon; Known benefit iron injection, how plain as the blood of Britain, technology is complicated, need repeatedly complexing, cost height and heavy metal content are higher, and the patent application of China (application number 88107217.6) discloses a kind of preparation method of Iron-Dextrin Complex, utilize molecular weight be 1000～1500 dextran through alkalization, complexing, refining forming, add 20% sodium hydroxide solution during complexing, make pH value progressively be increased to 7～8, the complexing time is 5～7 hours, such technology, the complexing time is long, and technology is still complicated.

The objective of the invention is to simplify synthesis technique, the Antai growth promotor that production cost is lower.

Synthesis technique of the present invention is: the employing molecular-weight average is 4000～9000 dextran, carries out acidolysis with hydrochloric acid, adds ferric chloride aqueous solutions again, after being 1～3 with strong base-weak acid salt adjustment solution to pH value therebetween, be heated to 50～70 ℃, insulation was not less than 1/3 hour, made its reaction; Add alkali then in reaction solution, transfer pH value to 9～12, and be heated to 100～120 ℃, be incubated 1～2 hour, make its complexing.Add ethanol after cooling and make it precipitation,, promptly make solid-state Antai growth promotor the throw out oven dry; Solid-state Antai growth promotor is mixed with the injection liquid of different concns with redistilled water, behind autoclaving, promptly becomes the Antai growth promotor injection.

Antai growth promotor is a kind of high organic iron complex injection liquid of concentration of iron that contains, and has prevention and treatment piglet and other livestock hypoferric anemia, the effect of enhancing body immunizing power and promotion growth.Stable breeding piglet hypoferric anemia has become generally acknowledged problem, the injection Antai growth promotor can effectively improve the oxyphorase value, mend iron piglet fur light, skin is ruddy, being full of animal spirits, use the thymidine method of mixing and measure the injection Antai growth promotor, confirm that the injection Antai growth promotor can effectively improve the immunologic function of piglet the piglet immune function influence.

Application examples:

With the piglet of birth after 2～3 days, be that unit is divided into two groups at random with the nest.Test group is in 1 milliliter of the Antai growth promotor of piglet buttocks muscle and deep injection iron content 150mg/ml, and blank group is not injected.Two groups of piglets all when test and 35 age in days time-divisions another name heavy, and add up its weightening finish and survival condition.It the results are shown in table 1 and table 2.

By table 1,2 as seen, improve 0.78 kilogram than average every the weightening finish of blank group behind the injection Antai growth promotor, improve 19.7%.Case fatality rate has descended 57.89% simultaneously.

Examine the piglet growth developmental state during whole test, visible general or locality toxic reaction all do not appear in the piglet of injection Antai growth promotor.At ruddy, the good bloom of whole skin lactation, spirit is active, appetite is good.And after ablactation, still keep good growing way.According to the weightening finish situation statistics (table 3) of 60 ages in days,

Show in piglet 25 days behind 35 ages in days of injecting Antai growth promotor and improve 0.9 kilogram, improve 12.7% than blank group weightening finish.

Table 3 60 age in days pig weightening finish situations statistics

For fully disclosing synthesis technique of the present invention, be illustrated for example below.

In the porcelain enamel barrel of 20L, add distilled water 3456ml, dissolve in solid iron trichloride 1890 grams again; Other gets a porcelain enamel barrel and adds distilled water 5184ml, and being heated to the back of boiling, to add molecular-weight average be 4000～9000 dextran, and it is concentrated hydrochloric acid acidifying in boiling water bath of 11mol/L that the dissolving back adds 36.7ml concentration.The mixing of two solution is mixed thoroughly, transfer PH=1～3, in stirring, drip 15% sodium carbonate solution 4L, and porcelain enamel barrel put be warming up to 65 ℃ in the water-bath, insulation half an hour, dripping concentration in stirring fast is the sodium hydroxide 1015g of 11mol/L, use again with the sodium hydroxide of concentration and regulate PH=10.5, be heated to 120 ℃, and kept 1.5 hours, make its complexing, cool off standby then.

With the refrigerative complex liquid through cloth filter back with 2 times of volume of ethanol precipitate, cloth is filtered, press dry throw out, with redistilled water throw out is dissolved fully, add 2.5 times of ethanol and make its redeposition once, the throw out that press dry is for the second time promptly got purer Iron Dextran through 50 ℃ of oven dry.

Preparation and sterilization: adding 1% phenol water (by the redistilled water preparation) weightmeasurement ratio in the solid Iron Dextran is 1g: 1.8ml, after dissolving fully in 90 ℃ of water-baths, measures iron-holder, is the injection liquid of 150mg/ml with redistilled water furnishing iron-holder then.Through No. 3 sintered glass funnel suction filtrations, packing, autoclaving was the Antai growth promotor injection liquid in 30 minutes under 110～120 ℃ temperature again.

Claims (4)

1, the synthesis technique of Antai growth promotor has complexing, refining, preparation and sterilization process, the invention is characterized in:

A. adopting the hcl acidifying molecular-weight average is 4000～9000 dextran, adds ferric chloride aqueous solutions again, adjusts solution to PH=1-3 with alkali therebetween; And the heating make the temperature of solution rise to 50～70 ℃, the insulation be not less than 1/3 hour, make its reaction.

B. add alkali and in reaction solution, transfer to PH=9-12, and be heated to 100 ℃～120 ℃, be incubated 1～2 hour, make its complexing.

2, Antai growth promotor synthesis technique according to claim 1, the molecular-weight average that it is characterized in that dextran is 6200.

3, Antai growth promotor synthesis technique according to claim 1 and 2 is characterized in that using 11mol/L hydrochloric acid; Alkali is 15% yellow soda ash and the sodium hydroxide of 11mol/L.

4,, it is characterized in that the complexing time is 1.5 hours according to claim 1 or 2 or 3 described Antai growth promotor synthesis techniques.