CN109699690A - A kind of botanical fungicide of anti-Erwinia carotorora - Google Patents
A kind of botanical fungicide of anti-Erwinia carotorora Download PDFInfo
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- CN109699690A CN109699690A CN201910038810.9A CN201910038810A CN109699690A CN 109699690 A CN109699690 A CN 109699690A CN 201910038810 A CN201910038810 A CN 201910038810A CN 109699690 A CN109699690 A CN 109699690A
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- smartweed
- volatile oil
- fungicide
- botanical fungicide
- erwinia
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Abstract
The present invention provides a kind of botanical fungicide of anti-Erwinia carotorora, which is using smartweed volatile oil as major ingredient, and configuration ethyl alcohol, Tween-20, water are prepared；The smartweed volatile oil is prepared via a method which to obtain: crushing smartweed, is sieved, being soaked in water and then obtaining smartweed volatile oil with extraction by steam distillation.The preparation method of the fungicide is simple, low in cost.The fungicide has significant bactericidal effect to the Erwinia.carotovora pv.carotovora (Erwinia carotorora) for causing soft rot of cabbage.After spraying Chinese cabbage with the fungicide, soft rot of cabbage can be played it is preferable prevent and treat effect, and product dispersibility, have good stability, free from environmental pollution, noresidue, the effect of may also function as green manure, is a kind of ideal botanical fungicide.
Description
Technical field
It is specifically a kind of using smartweed volatile oil as the anti-soft rot of cabbage of primary raw material the present invention relates to botanical fungicide
The botanical fungicide of bacterium.
Background technique
Chinese cabbage is Northeast China and North China's winter, spring main vegetables.Soft rot of cabbage is commonly called as rotten pimple, rots
Disease is a kind of destructive disease in Chinese cabbage production, and production loss is generally 5%~20%, and serious plot even has no harvest.Chinese cabbage
Soft rot is invaded by Erwinia.carotovora pv.carotovora (Erwinia carotorora) by Chinese cabbage wound, or passes through rainwater, insect
Caused by propagation etc..Germ will cause Chinese cabbage and rot after infecting, the rotten Chinese cabbage disease leaf dehydration of forth infection dries out, in half
Thin transparent paper-like is affixed on leaf-head, and final entire dish ball softening rots, gives out a foul smell.In addition, in addition to the field growing phase, Chinese cabbage
Bacteria erwinia can also cause Chinese cabbage to be rotted in transport and storage period, cause damages.
Currently, for the medical treatment of Erwinia carotorora, it is main still to utilize chemical bactericide, such as network ammonia copper, enemy gram
Pine etc..It is well known, however, that chemical bactericide will cause soil and water resource pollution, the harm such as pesticide residue also will cause agriculture
Product edible safety hidden danger.As people are increasingly enhanced environmental protection, food and agricultural product edible safety consciousness, it is badly in need of research and opens
Send out a kind of inhibit Erwinia carotorora efficient, free of contamination botanical fungicide, for soft rot of cabbage green prevent and treat provide
One new approach.
Smartweed (Polygonum orientale L.) belongs to large-scale herbaceous plant, up to two meters, is distributed widely in complete
State various regions.Smartweed growth is very rapid, wild and cultivation can vigorous growth, adaptability is extremely strong.Since smartweed yield is high, it is easy to
Materials, can provide stable source for the research and development of botanical fungicide.The experimental results showed that smartweed volatile oil is to Chinese cabbage
Bacteria erwinia has strong fungistatic effect.Have using smartweed volatile oil as the botanical fungicide that major ingredient configures to soft rot of cabbage good
Good preventive effect and therapeutic effect.Therefore, research and development smartweed volatile oil botanical fungicide can be Erwinia carotorora
Green prevention and treatment provides effective way.
Summary of the invention
The purpose of the present invention is to provide a kind of using smartweed volatile oil as the green of the anti-Erwinia carotorora of primary raw material
Botanical fungicide.
A kind of botanical fungicide of anti-Erwinia carotorora provided by the invention, contains smartweed volatile oil.The smartweed
The ingredient of volatile oil is mainly the compounds such as alcohols, alkanes, terpene.These ingredients are pollution-free to crops and soil environment,
To human health, environmentally protective fungicide product can be used as.
A kind of botanical fungicide of anti-Erwinia carotorora, component by weight percentage: smartweed volatile oil 2%~
4%, Tween-20 2%~8%, ethyl alcohol 8%~15%, water surplus.
Preferably, component is by weight percentage: smartweed volatile oil 3%, Tween-20 8%, ethyl alcohol 12%, more than water
Amount.
The method of the smartweed volatile oil as follows is prepared: (1) acquiring smartweed, clean, under natural conditions
It dries, smash it through 60~100 meshes；(2) it takes smartweed powder to add 8~12 times of water to mix by quality, impregnates 0~24 hour, water
Steam distillation method is extracted 3~9 hours, and smartweed volatile oil is obtained.
Preferably, the method for the smartweed volatile oil as follows is prepared: (1) smartweed is acquired, is cleaned, from
It dried under the conditions of so, smash it through 80 meshes；(2) it takes smartweed powder to add 10 times of water to mix by quality, impregnates 3 hours, vapor
The way of distillation is extracted 8 hours, and smartweed volatile oil is obtained.
A kind of application method of the botanical fungicide of anti-Erwinia carotorora is diluted with water smartweed volatilization oil botanical and kills
0~320 times of microbial inoculum, at Chinese cabbage foliar spray 1~2 time.
Preferably, 0~80 times of smartweed volatile oil botanical fungicide is diluted with water, at Chinese cabbage foliar spray 1 time.
The effect of each auxiliary material in the present invention: ethyl alcohol, solvent, cheap, small toxicity can preferably dissolve smartweed volatilization
Oil.With ethyl alcohol, methanol, DMSO, petroleum ether, acetone, n-Butanol soluble smartweed volatile oil, according to dissolubility, diffusivity and environment
Safety, screening show that ethyl alcohol is the solvent of smartweed volatile oil botanical fungicide.With Tween-20, Tween-80, OP-10,
Span-80, PEG-600, PEG-400, NP-40 are emulsifier, configure botanical fungicide.Sterilize virulence experiment the result shows that,
OP-10 and the plant source missible oil of Tween-20 configuration have good bactericidal effect.But since OP-10 is not degradable, to environment and
Organism is likely to result in adverse effect, and Tween-20 has good emulsification, peptizaiton, therefore final choice Tween-20 is
The emulsifier of smartweed volatile oil botanical fungicide.
Beneficial effects of the present invention:
Virulence sterilization experiment shows that smartweed volatile oil botanical fungicide has significant bactericidal effect, and inhibition zone is straight
Diameter is 14.18~16.88mm.Preventive effect of the smartweed volatile oil botanical fungicide to soft rot of cabbage: it is within the 3rd day
83.33%~98.67%, the 5th day is 50%~94.67%；To the therapeutic effect of soft rot of cabbage: the 3rd day for 75%~
97.33%, the 5th day is 50%~94.67%.Especially smartweed volatile oil botanical fungicide stoste, to soft rot of cabbage
Prevention and therapeutic effect can reach 90%, illustrate that it has extraordinary control efficiency to soft rot of cabbage.Dispersibility experiment table
Bright, smartweed volatile oil botanical fungicide can be spread rapidly, good dispersion.Stability experiment shows smartweed volatilization oil botanical
Fungicide is at a temperature of 32 DEG C ± 2 DEG C, static 1 hour, does not precipitate or missible oil is precipitated, stability is good.Ultraviolet light stability is real
It tests and shows that smartweed volatile oil botanical fungicide has preferable environmental stability to ultraviolet light, do not need to be protected from light place in the application
Reason.
To sum up, smartweed volatile oil botanical fungicide provided by the invention is obvious to the bactericidal effect of Erwinia carotorora,
It is significant to the control efficiency of soft rot of cabbage, and product is dispersed, has good stability, it is free from environmental pollution, it may also function as green manure
Effect is a kind of ideal botanical fungicide.
Detailed description of the invention
Fungistatic effect of Fig. 1 smartweed volatile oil botanical fungicide to Erwinia carotorora
Fig. 2 smartweed volatile oil the 5th day preventive effect of botanical fungicide
Fig. 3 smartweed volatile oil the 5th day therapeutic effect of botanical fungicide
Specific embodiment:
Embodiment 1,2,3 is the preparation of smartweed volatile oil botanical fungicide；Embodiment 4 is that smartweed volatilization oil botanical kills
The toxicity test of microbial inoculum；Embodiment 5 is the preventive effect of smartweed volatile oil botanical fungicide；Embodiment 6 is smartweed volatile oil
The therapeutic effect of botanical fungicide；Embodiment 7 is that the property of smartweed volatile oil botanical fungicide measures.
Embodiment 1
The preparation step of smartweed volatile oil:
(1) smartweed is acquired, is cleaned, dried under natural conditions, smash it through 60 meshes；
(2) add 8 times of hard water mixing by the quality of smartweed powder, do not impregnate, extraction by steam distillation 3 hours, obtain smartweed
Volatile oil.
The preparation of smartweed volatile oil botanical fungicide:
Raw material proportioning: 2 kilograms of smartweed volatile oil, 2 kilograms of Tween-20,8 kilograms of ethyl alcohol, 88 kilograms of hard water.Match by raw material
Than being uniformly mixed, it is packaged to be smartweed volatile oil botanical fungicide product.
Embodiment 2
The preparation step of smartweed volatile oil:
(1) smartweed is acquired, is cleaned, dried under natural conditions, smash it through 80 meshes；
(2) add 10 times of hard water mixing by the quality of smartweed powder, impregnate 3 hours, extraction by steam distillation 8 hours, obtain
Smartweed volatile oil.
The preparation of smartweed volatile oil botanical fungicide:
Raw material proportioning: 3 kilograms of smartweed volatile oil, 8 kilograms of Tween-20,12 kilograms of ethyl alcohol, 77 kilograms of hard water.Match by raw material
Than being uniformly mixed, it is packaged to be smartweed volatile oil botanical fungicide product.
Embodiment 3
The preparation step of smartweed volatile oil:
(1) smartweed is acquired, is cleaned, dried under natural conditions, smash it through 100 meshes；
(2) add 12 times of hard water mixing by the quality of smartweed powder, impregnate 24 hours, extraction by steam distillation 9 hours, obtain
Smartweed volatile oil.
The preparation of smartweed volatile oil botanical fungicide:
Raw material proportioning: 4 kilograms of smartweed volatile oil, 8 kilograms of Tween-20,15 kilograms of ethyl alcohol, 73 kilograms of hard water.Match by raw material
Than being uniformly mixed, it is packaged to be smartweed volatile oil botanical fungicide product.
Embodiment 4
Smartweed volatile oil botanical fungicide is prepared by embodiment 2, is control with auxiliary material, poison is carried out to Erwinia carotorora
Power bacteriostatic experiment, as a result as shown in Figure 1, in figure: A is the fungistatic effect of smartweed volatile oil botanical fungicide；B is control auxiliary material
Fungistatic effect.Table 1 is the result shows that the smartweed volatile oil botanical fungicide under each extension rate has Erwinia carotorora
Apparent bactericidal effect, antibacterial circle diameter is between 14.18mm~16.88mm.
Fungistatic effect of the 1 smartweed volatile oil botanical fungicide of table to Erwinia carotorora
Embodiment 5
Smartweed volatile oil botanical fungicide is prepared according to embodiment 2, hard water dilutes 0~320 times, sparges on Chinese cabbage.
After ten minutes, 100 μ L of Erwinia carotorora is accessed by general wound inoculation method to each processing cabbage leaf, in the 3rd day, the 5th
It is observed, and result is as shown in Fig. 2, in figure within the 5th day: A is the rotten level of control group Chinese cabbage；B is smartweed volatile oil plant
Source bactericide dilutes 0 times of rotten level for acting on lower Chinese cabbage；C is white under 40 times of effects of smartweed volatile oil botanical fungicide dilution
The rotten level of dish；D is that smartweed volatile oil botanical fungicide dilutes 80 times of rotten levels for acting on lower Chinese cabbage；E waves for smartweed
Hair oil botanical fungicide dilutes 160 times of rotten levels for acting on lower Chinese cabbage；F is the dilution of smartweed volatile oil botanical fungicide
The rotten level of the lower Chinese cabbage of 320 times of effects.Not spray the Chinese cabbage of smartweed volatile oil botanical fungicide for control, by following public affairs
Show the preventive effect (table 2) for calculating smartweed volatile oil botanical fungicide.3rd day preventive effect be 83.33%~
98.67%, the 5th day preventive effect is 50%~94.67%.
The preventive effect of 2 smartweed volatile oil botanical fungicide of table
Embodiment 6
Each processing cabbage leaf accesses 100 μ L of Erwinia carotorora by general wound inoculation method.It is prepared according to embodiment 2
Smartweed volatile oil botanical fungicide, hard water dilute 0~320 times.Chinese cabbage connects bacterium after ten minutes, and smartweed volatilization oil botanical is killed
Microbial inoculum sparges on Chinese cabbage.Observed in the 3rd day, the 5th day, result is as shown in figure 3, in figure within the 5th day: A is control group Chinese cabbage
Rotten level；B is that smartweed volatile oil botanical fungicide dilutes 0 times of rotten level for acting on lower Chinese cabbage；C is smartweed volatile oil
Botanical fungicide dilutes 40 times of rotten levels for acting on lower Chinese cabbage；D is that smartweed volatile oil botanical fungicide dilutes 80 times of works
With the rotten level of lower Chinese cabbage；E is that smartweed volatile oil botanical fungicide dilutes 160 times of rotten levels for acting on lower Chinese cabbage；F is
Smartweed volatile oil botanical fungicide dilutes 320 times of rotten levels for acting on lower Chinese cabbage.Not spray smartweed volatilization oil botanical
The Chinese cabbage of fungicide is control, and the therapeutic effect (table 3) of smartweed volatile oil botanical fungicide is calculated by following publicity.3rd day
Therapeutic effect be 75%~97.33%, the 5th day therapeutic effect is 50%~94.67%.
The therapeutic effect of 3 smartweed volatile oil botanical fungicide of table
Embodiment 7
Dispersibility test, smartweed volatile oil botanical fungicide can be spread rapidly, good dispersion.Stability test, smartweed
Volatile oil botanical fungicide is at a temperature of 32 DEG C ± 2 DEG C, static 1 hour, does not precipitate or missible oil is precipitated, stability is good.It is purple
Outer light stability test, smartweed volatile oil botanical fungicide have preferable environmental stability to ultraviolet light, are not required in the application
It is protected from light processing.
Claims (6)
1. a kind of botanical fungicide of anti-Erwinia carotorora, it is characterised in that contain smartweed volatile oil.
2. a kind of botanical fungicide of anti-Erwinia carotorora as described in claim 1, which is characterized in that component is by weight
Percentages: smartweed volatile oil 2%~4%, Tween-20 2%~8%, ethyl alcohol 8%~15%, water surplus；
The method of the smartweed volatile oil as follows is prepared: (1) smartweed acquired, is cleaned, dried under natural conditions,
Smash it through 60~100 meshes；(2) it takes smartweed powder to add 8~12 times of water to mix by quality, impregnates 0~24 hour, vapor steams
It evaporates method to extract 3~9 hours, obtains smartweed volatile oil.
3. the botanical fungicide of anti-Erwinia carotorora as claimed in claim 2, which is characterized in that component percentage by weight
Than meter: smartweed volatile oil 3%, Tween-20 8%, ethyl alcohol 12%, water surplus.
4. the botanical fungicide of anti-Erwinia carotorora as claimed in claim 3, which is characterized in that the smartweed volatile oil
Method as follows is prepared: (1) acquiring smartweed, clean, dry under natural conditions, smash it through 80 meshes；(2)
It takes smartweed powder to add 10 times of water to mix by quality, impregnates 3 hours, extraction by steam distillation 8 hours, obtain smartweed volatile oil.
5. a kind of application method of the botanical fungicide of anti-Erwinia carotorora, which is characterized in that right such as is diluted with water and wants
0~320 times of the described in any item fungicide of 1-4 are sought, at Chinese cabbage foliar spray 1~2 time.
6. the application method of anti-Erwinia carotorora botanical fungicide as claimed in claim 5, which is characterized in that dilute with water
Described 0~80 times of fungicide is released, at Chinese cabbage foliar spray 1 time.
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| CN201910038810.9A CN109699690B (en) | 2019-01-16 | 2019-01-16 | Botanical fungicide for resisting cabbage soft rot |
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| CN201910038810.9A CN109699690B (en) | 2019-01-16 | 2019-01-16 | Botanical fungicide for resisting cabbage soft rot |
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