CN109694900A - A kind of indoor rapid identification method of maize diseases resistance - Google Patents
A kind of indoor rapid identification method of maize diseases resistance Download PDFInfo
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- CN109694900A CN109694900A CN201910122344.2A CN201910122344A CN109694900A CN 109694900 A CN109694900 A CN 109694900A CN 201910122344 A CN201910122344 A CN 201910122344A CN 109694900 A CN109694900 A CN 109694900A
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- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 134
- 201000010099 disease Diseases 0.000 title claims abstract description 83
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title claims abstract description 33
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 title claims abstract description 29
- 235000009973 maize Nutrition 0.000 title claims abstract description 29
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 105
- 235000005822 corn Nutrition 0.000 claims abstract description 105
- 241000228197 Aspergillus flavus Species 0.000 claims abstract description 91
- 239000000725 suspension Substances 0.000 claims abstract description 53
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 39
- 238000011534 incubation Methods 0.000 claims abstract description 34
- 238000002360 preparation method Methods 0.000 claims abstract description 26
- 241000228212 Aspergillus Species 0.000 claims description 28
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000009736 wetting Methods 0.000 claims description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 13
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 13
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 13
- 239000008223 sterile water Substances 0.000 claims description 12
- 238000012545 processing Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims 1
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- 238000012797 qualification Methods 0.000 abstract description 5
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- 239000000843 powder Substances 0.000 description 5
- 229930195730 Aflatoxin Natural products 0.000 description 4
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 description 4
- 239000005409 aflatoxin Substances 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
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Abstract
The invention belongs to corn aspergillus flavus Ear rot resistance technical fields, disclose a kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in respectively on each seed for trying corn sample of every kind of disinfection, constant temperature incubation obtains the corn variety of introduced disease；D, use the susceptible variety that onset grade is 9 grades as control, determine that the average sick grade of the corn variety of every kind of introduced disease realizes the identification of corn aspergillus flavus Ear rot resistance according to the average disease scale resistance.The present invention has the characteristics that qualification cycle is short, appraisal cost is low, result is accurate.
Description
Technical field
The invention belongs to corn aspergillus flavus Ear rot resistance identification technology fields, and in particular to a kind of maize diseases resistance
Indoor rapid identification method.
Background technique
Maize kernel rot is a maize diseases close with human health.Maize production area from all parts of the world is all
There is generation.The fungi complex of this disease can produce several mycotoxins (mycotoxin), such as aflatoxin and Fu Ma
The chemical substance of the influence humans and animals health such as toxin.
Aspergillus flavus (Aflavus) in Aspergillus is not only caused harm the plurality of cereals such as corn, also generation toxic metabolic products
Aflatoxin causes people and domestic animal, poultry poisoning.Medical research shows to eat the aflatoxin containing low concentration for a long time
The main reason for food is considered as leading to liver cancer, gastric cancer, the diseases such as intestinal cancer.
Corn ear and seed can be aggrieved, symptom be killed fruit ear or seed yellow, yellow green, yellowish-brown occur mould
Layer, the i.e. mycelium, conidiophore of pathogen and conidium.Infected seed is matt, not full, rots, internal hollow, Chang Wei
The mycelia of intertexture and spore are filled.Bract is often run through by intensive mycelia in fruit ear disease portion, is bonded together and is affixed on fruit ear not
It is easily peelable.
Corn once infects the disease, and caused aflatoxin accumulates problem, will lead to food pollution, food pollution,
And human body is entered by food chain, and then causes human lesion.Toxin in corn is exceeded to make Feed Manufacturing producer be reluctant to receive
Local corn is purchased as feed processing raw material, causes local corn commodity rate and price low.Currently, chemical control is to the disease
Evil is substantially invalid.Therefore, plantation disease-resistant variety is to solve the most economical effective approach of the disease.The screening of disease-resistant variety needs letter
Single, easy, accurate Resistance Identification technology.
However, existing corn aspergillus flavus Ear rot resistance identification technology is generally crop field artificial inoculation technique, pass through jade
Rice kind stabs seed inoculation with band acicula under conditions of field planting, in the corn ear spinning phase, until fruit ear maturation is received
Resistance level investigation is carried out when obtaining to be identified；The defect of the prior art is to need in field planting corn variety, heavy workload,
It is at high cost, the period is long, qualification result climate condition influences big.
Summary of the invention
In order to solve the above problems existing in the present technology, it is an object of that present invention to provide a kind of rooms of maize diseases resistance
Interior rapid identification method.
The technical scheme adopted by the invention is as follows:
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
Further, the step A includes: that aspergillus flavus strain is inoculated on PDA plate, and constant temperature incubation goes out aspergillus flavus spore
Son obtains Aspergillus flavus spore suspension after being mixed with sterile water with the aspergillus spore.
Further, the temperature of constant temperature is 20 DEG C -25 DEG C in the step A；It is yellow in the Aspergillus flavus spore suspension
The quantity of aspergillus spore is 1 × 105A/mL-1 × 107A/mL.
Further, the step B includes: that each seed to each for trying corn variety carries out disinfection processing, sterile water
Cleaning, sterilizing filter paper obtained after draining a variety of disinfections for try corn variety sample.
Further, the disinfection treatment of the step B include by it is each for try corn variety each seed successively alcohol,
The step of sodium hypochlorite impregnates.
Further, the mass fraction of alcohol is 75% in the step B, and the mass fraction of sodium hypochlorite is 3%；It is each to supply
Each seed of examination corn variety is 30s with the time that alcohol impregnates；Respectively for each seed sodium hypochlorite of examination corn variety
The time of immersion is 3min.
Further, the specific steps of the step C: drawing quantitative Aspergillus flavus spore suspension and soak sterilizing filter paper,
Obtain wetting filter paper；It will be placed on wetting filter paper for each seed for trying corn sample, constant temperature incubation obtains the jade of introduced disease
Rice kind.
Further, the temperature of constant temperature incubation is 20 DEG C -25 DEG C in the step C；The step C soaks a sterilizing filter
The volume for the Aspergillus flavus spore suspension that paper is drawn is 5mL；The seed quantity that every wetting filter paper is placed is 10.
The invention has the benefit that a kind of indoor rapid identification method of maize diseases resistance of the invention is using indoor
The mode of identification avoids land used expense, the cost of cultivation management expense that crop field identification needs to be planted in field；Of the invention
The qualification cycle of identification method is 1 week, compared with crop field identifies that a season needs 4 months, greatly reduces the time cycle；The present invention
Identification method cultivate in constant incubator indoors, avoid field weather conditions it is unstable caused by morbidity is insufficient etc. leads
The error of the result inaccuracy of cause；The present invention can greatly reduce a large amount of Aspergillus flavus of field test generation, reduce to environment
Pollution.Easy to operate present invention saves human and material resources cost, qualification result is more stable, credible.To which the present invention has
The feature that qualification cycle is short, appraisal cost is low and result is accurate.
Specific embodiment
Further explaination is done to the present invention combined with specific embodiments below.
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
Further, the step A includes: that aspergillus flavus strain is inoculated on PDA plate, and constant temperature incubation goes out aspergillus flavus spore
Son obtains Aspergillus flavus spore suspension after being mixed with sterile water with the aspergillus spore.
Further, the temperature of constant temperature is 20 DEG C -25 DEG C in the step A；It is yellow in the Aspergillus flavus spore suspension
The quantity of aspergillus spore is 1 × 105A/mL-1 × 107A/mL.
Further, the step B includes: that each seed to each for trying corn variety carries out disinfection processing, sterile water
Cleaning, sterilizing filter paper obtained after draining a variety of disinfections for try corn variety sample.
Further, the disinfection treatment of the step B include by it is each for try corn variety each seed successively alcohol,
The step of sodium hypochlorite impregnates.
Further, the mass fraction of alcohol is 75% in the step B, and the mass fraction of sodium hypochlorite is 3%；It is each to supply
Each seed of examination corn variety is 30s with the time that alcohol impregnates；Respectively for each seed sodium hypochlorite of examination corn variety
The time of immersion is 3min.
Further, the specific steps of the step C: drawing quantitative Aspergillus flavus spore suspension and soak sterilizing filter paper,
Obtain wetting filter paper；It will be placed on wetting filter paper for each seed for trying corn sample, constant temperature incubation obtains the jade of introduced disease
Rice kind.
Further, the temperature of constant temperature incubation is 20 DEG C -25 DEG C in the step C；The step C soaks a sterilizing filter
The volume for the Aspergillus flavus spore suspension that paper is drawn is 5mL；The seed quantity that every wetting filter paper is placed is 10.
Embodiment 1
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
Embodiment 2
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
The step A includes: that aspergillus flavus strain is inoculated on PDA plate, and constant temperature incubation goes out aspergillus spore, and use is sterile
Water obtains Aspergillus flavus spore suspension after mixing with the aspergillus spore.
PDA in the present invention be potato dextrose agar (potato 200g, glucose 15g, agar powder 18g,
Water 1000mL), it is used by turning out aspergillus spore as subsequent inoculations step after PDA plate inoculation aspergillus flavus strain.
Embodiment 3
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
The step A includes: that aspergillus flavus strain is inoculated on PDA plate, and constant temperature incubation goes out aspergillus spore, and use is sterile
Water obtains Aspergillus flavus spore suspension after mixing with the aspergillus spore.
PDA in the present invention be potato dextrose agar (potato 200g, glucose 15g, agar powder 18g,
Water 1000mL), it is used by turning out aspergillus spore as subsequent inoculations step after PDA plate inoculation aspergillus flavus strain.
The temperature of constant temperature is 20 DEG C in the step A；The quantity of aspergillus spore in the Aspergillus flavus spore suspension
It is 1 × 105A/mL.
Embodiment 4
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
The step A includes: that aspergillus flavus strain is inoculated on PDA plate, and constant temperature incubation goes out aspergillus spore, and use is sterile
Water obtains Aspergillus flavus spore suspension after mixing with the aspergillus spore.
PDA in the present invention be potato dextrose agar (potato 200g, glucose 15g, agar powder 18g,
Water 1000mL), it is used by turning out aspergillus spore as subsequent inoculations step after PDA plate inoculation aspergillus flavus strain.
The temperature of constant temperature is 23 DEG C in the step A；The quantity of aspergillus spore in the Aspergillus flavus spore suspension
It is 1 × 107A/mL.
Embodiment 5
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
The step A includes: that aspergillus flavus strain is inoculated on PDA plate, and constant temperature incubation goes out aspergillus spore, and use is sterile
Water obtains Aspergillus flavus spore suspension after mixing with the aspergillus spore.
PDA in the present invention be potato dextrose agar (potato 200g, glucose 15g, agar powder 18g,
Water 1000mL), it is used by turning out aspergillus spore as subsequent inoculations step after PDA plate inoculation aspergillus flavus strain.
The temperature of constant temperature is 25 DEG C in the step A；The quantity of aspergillus spore in the Aspergillus flavus spore suspension
It is 1 × 106A/mL.
Embodiment 6
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
The step A includes: that aspergillus flavus strain is inoculated on PDA plate, and constant temperature incubation goes out aspergillus spore, and use is sterile
Water obtains Aspergillus flavus spore suspension after mixing with the aspergillus spore.
PDA in the present invention be potato dextrose agar (potato 200g, glucose 15g, agar powder 18g,
Water 1000mL), it is used by turning out aspergillus spore as subsequent inoculations step after PDA plate inoculation aspergillus flavus strain.
The temperature of constant temperature is 25 DEG C in the step A；The quantity of aspergillus spore in the Aspergillus flavus spore suspension
It is 1 × 106A/mL.
The step B includes: that each seed to each for trying corn variety carries out disinfection processing, sterile water wash, sterilizing
Filter paper obtained after draining a variety of disinfections for try corn variety sample；The disinfection treatment of the step B includes by each for trying corn
The step of each seed of kind successively uses alcohol, sodium hypochlorite to impregnate；The mass fraction of alcohol is 75% in the step B,
The mass fraction of sodium hypochlorite is 3%；It is respectively 30s for trying the time that each seed alcohol of corn variety impregnates；Respectively for examination
Each seed of corn variety is 3min with the time that sodium hypochlorite impregnates.
Embodiment 7
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
The specific steps of the step C: it draws quantitative Aspergillus flavus spore suspension and soaks sterilizing filter paper, obtain wetting filter
Paper；It will be placed on wetting filter paper for each seed for trying corn sample, constant temperature incubation obtains the corn variety of introduced disease.
The temperature of constant temperature incubation is 20 DEG C in the step C；The step C soaks the aspergillus flavus that a sterilizing filter paper is drawn
The volume of bacterium spore suspension is 5mL；The seed quantity that every wetting filter paper is placed is 10.
Embodiment 8
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
The specific steps of the step C: it draws quantitative Aspergillus flavus spore suspension and soaks sterilizing filter paper, obtain wetting filter
Paper；It will be placed on wetting filter paper for each seed for trying corn sample, constant temperature incubation obtains the corn variety of introduced disease.
The temperature of constant temperature incubation is 22 DEG C in the step C；The step C soaks the aspergillus flavus that a sterilizing filter paper is drawn
The volume of bacterium spore suspension is 5mL；The seed quantity that every wetting filter paper is placed is 10.
Embodiment 9
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of A, preparation Aspergillus flavus spore suspension；
B, preparation disinfection for try corn variety sample；C, Aspergillus flavus spore suspension is seeded in the beautiful for examination of every kind of disinfection respectively
On each seed of rice sample, constant temperature incubation obtains the corn variety of introduced disease；It D, the use of onset grade is 9 grades susceptible
Kind determines the average sick grade of the corn variety of every kind of introduced disease as control, real according to the average disease scale resistance
The identification of existing corn aspergillus flavus Ear rot resistance.
The specific steps of the step C: it draws quantitative Aspergillus flavus spore suspension and soaks sterilizing filter paper, obtain wetting filter
Paper；It will be placed on wetting filter paper for each seed for trying corn sample, constant temperature incubation obtains the corn variety of introduced disease.
The temperature of constant temperature incubation is 25 DEG C in the step C；The step C soaks the aspergillus flavus that a sterilizing filter paper is drawn
The volume of bacterium spore suspension is 5mL；The seed quantity that every wetting filter paper is placed is 10.
Embodiment 10
A kind of indoor rapid identification method of maize diseases resistance, comprising steps of
Bacterium source preparation method: the aspergillus flavus strain of preservation is inoculated on PDA plate, constant temperature incubation 5d at 25 DEG C, to Huang
After Aspergillus covers with plate, with aspergillus spore under sterile washing, is counted under the microscope with blood counting chamber, bacterium solution is diluted
At 1 × 106A/ml is stand-by.
For examination corn sample processing: it chooses full seed, seed in the same size, healthy appearance and carries out disinfection processing, elder generation
It is impregnated one minute with 75% alcohol, is then impregnated three minutes with 3% sodium hypochlorite, finally wash with distilled water three times, be placed on and go out
It drains away the water on bacterium filter paper spare.
Inoculation method: placing one layer of sterilizing filter paper in culture dish bottom, draw 5mL spore suspension uniform wet filter paper,
The seed sterilized is put on the wet filter paper in culture dish, every ware uniformly places 10, covers lid, is put into constant temperature incubation
It is cultivated at 25 DEG C in case.Observation is taken pictures after 7 days, records incidence.
In addition, step C of the invention can be inoculated with following manner, replaced using the PDA plate for being vaccinated with bacterial strain
Sterilizing filter paper, or with being placed in sterilizing filter paper after being soaked in aspergillus flavus bacterium solution for examination corn kernel sample, or by Corn Seeds
After grain sample is placed in sterilizing filter paper, bacterium solution is sprayed on seed and is inoculated with, 3 kinds of methods can make seed fall ill.
Use the susceptible variety that onset grade is 9 grades as control, determines being averaged for the corn variety of every kind of introduced disease
Sick grade realizes the identification of corn aspergillus flavus Ear rot resistance according to the average disease scale resistance.
Comparative example
Control setting: it uses susceptible variety as control, when reaching 9 grade standard wait compare morbidity, institutes an inquiry for sample
Product incidence.
Sick grade judgment criteria: such as the following table 1.
1 corn aspergillus flavus resisting ear rot of table identifies state of an illness partition of the level
| Severity Scaling | Description |
| 1 | Onset area accounts for the seed gross area 0%~1% |
| 3 | Onset area accounts for the seed gross area 2%~10% |
| 5 | Onset area accounts for the seed gross area 11%~25% |
| 7 | Onset area accounts for the seed gross area 26%~50% |
| 9 | Onset area accounts for the seed gross area 51%~100% |
It will be placed in culture dish for the seed for trying 26 corn varieties, 7 days after inoculation, investigation result such as the following table 2.Under
The result of table 2, can be by different jade according to the anti-sense criteria for classifying of kind it is found that 26 kind disease grades minimum 3.9, are up to 9.0
The resistance of rice kind gives full expression to.
Wherein average sick grade is the sum of sick grade of all seeds for trying corn sample of each kind and each kind
The ratio of the quantity of all seeds.
The average sick grade result of 2 26 corn variety indoor inoculation identification aspergillus flavus of table
Specific resistance level division is shown in such as the following table 3.
3 corn of table divides the resistance level of Ear rot resistance
| Average state of an illness rank | Resistance |
| ≤1.5 | Highly resistance Highly resistant (HR) |
| 1.6~3.5 | Anti- Resistant (R) |
| 3.6~5.5 | In anti-Moderately resistant (MR) |
| 5.6~7.5 | Feel Susceptible (S) |
| 7.6~9.0 | Height sense Highly susceptible (HS) |
To which the present invention uses susceptible variety to realize indoor mirror using above-mentioned indoor rapid identification method as control
It is fixed, greatly reduce the time cycle；Avoid weather conditions it is unstable caused by morbidity it is insufficient it is equal caused by result inaccuracy
Error；The pollution to environment is reduced simultaneously.
The present invention is not limited to above-mentioned optional embodiment, anyone can show that other are each under the inspiration of the present invention
The product of kind form.Above-mentioned specific embodiment should not be understood the limitation of pairs of protection scope of the present invention, protection of the invention
Range should be subject to be defined in claims, and specification can be used for interpreting the claims.
Claims (8)
1. a kind of indoor rapid identification method of maize diseases resistance, it is characterised in that: comprising steps of
A, Aspergillus flavus spore suspension is prepared；
B, preparation disinfection for try corn variety sample；
C, Aspergillus flavus spore suspension is seeded in respectively on each seed for trying corn sample of every kind of disinfection, constant temperature training
It supports, obtains the corn variety of introduced disease；
D, use the susceptible variety that onset grade is 9 grades as control, determine the average disease of the corn variety of every kind of introduced disease
Grade realizes the identification of corn aspergillus flavus Ear rot resistance according to the average disease scale resistance.
2. a kind of indoor rapid identification method of maize diseases resistance according to claim 1, it is characterised in that: the step
Rapid A includes: that aspergillus flavus strain is inoculated on PDA plate, and constant temperature incubation goes out aspergillus spore, with sterile water and the aspergillus flavus
Aspergillus flavus spore suspension is obtained after spore mixing.
3. a kind of indoor rapid identification method of maize diseases resistance according to claim 2, it is characterised in that: the step
The temperature of constant temperature is 20 DEG C -25 DEG C in rapid A；The quantity of aspergillus spore is 1 × 10 in the Aspergillus flavus spore suspension5
A/mL-1 × 107A/mL.
4. a kind of indoor rapid identification method of maize diseases resistance according to claim 3, it is characterised in that: the step
Rapid B includes: that carry out disinfection processing, sterile water wash, sterilizing filter paper of each seed to each for trying corn variety obtains after draining
A variety of disinfections for try corn variety sample.
5. a kind of indoor rapid identification method of maize diseases resistance according to claim 4, it is characterised in that: the step
The disinfection treatment of rapid B includes the steps that successively alcohol, sodium hypochlorite impregnate each seed by each for trying corn variety.
6. a kind of indoor rapid identification method of maize diseases resistance according to claim 5, it is characterised in that: the step
The mass fraction of alcohol is 75% in rapid B, and the mass fraction of sodium hypochlorite is 3%；Respectively for each seed of examination corn variety
The time that alcohol impregnates is 30s；It is respectively 3min for trying the time that each seed sodium hypochlorite of corn variety impregnates.
7. a kind of indoor rapid identification method of maize diseases resistance according to claim 6, it is characterised in that: the step
The specific steps of rapid C: it draws quantitative Aspergillus flavus spore suspension and soaks sterilizing filter paper, obtain wetting filter paper；It will be for trying corn
Each seed of sample is placed on wetting filter paper, and constant temperature incubation obtains the corn variety of introduced disease.
8. a kind of indoor rapid identification method of maize diseases resistance according to claim 7, it is characterised in that: the step
The temperature of constant temperature incubation is 20 DEG C -25 DEG C in rapid C；It is outstanding that the step C soaks the Aspergillus flavus spore that a sterilizing filter paper is drawn
The volume of supernatant liquid is 5mL；The seed quantity that every wetting filter paper is placed is 10.
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| CN111411143A (en) * | 2020-04-30 | 2020-07-14 | 广东省农业科学院作物研究所 | Method for identifying corn bacterial stripe disease caused by Pantoea ananatis |
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| EP2011396A1 (en) * | 2007-07-03 | 2009-01-07 | Bayer CropScience AG | Active compound combinations |
| CN107870224A (en) * | 2017-11-03 | 2018-04-03 | 安徽省农业科学院烟草研究所 | A kind of field identification method for evaluating corn aspergillus flavus infection resistance ability |
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| EP2011396A1 (en) * | 2007-07-03 | 2009-01-07 | Bayer CropScience AG | Active compound combinations |
| CN107870224A (en) * | 2017-11-03 | 2018-04-03 | 安徽省农业科学院烟草研究所 | A kind of field identification method for evaluating corn aspergillus flavus infection resistance ability |
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| CN111411143A (en) * | 2020-04-30 | 2020-07-14 | 广东省农业科学院作物研究所 | Method for identifying corn bacterial stripe disease caused by Pantoea ananatis |
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