CN109692761A - A kind of platelet rich plasma extraction element and its method - Google Patents
A kind of platelet rich plasma extraction element and its method Download PDFInfo
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- CN109692761A CN109692761A CN201811594893.1A CN201811594893A CN109692761A CN 109692761 A CN109692761 A CN 109692761A CN 201811594893 A CN201811594893 A CN 201811594893A CN 109692761 A CN109692761 A CN 109692761A
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- sheath body
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
- B04B9/02—Electric motor drives
- B04B9/04—Direct drive
Abstract
The present invention relates to the field of medical instrument technology, in particular to a kind of platelet rich plasma extraction element and its method, device include cabinet and control system, and supporting table and equipment box are fixedly connected in cabinet, is fixedly installed with driving motor and pallet in supporting table；Equipment upper box part is equipped with vibrating disk, and vibrating disk is connected with driving device；Using the extracting method of this device, include the following steps, before sample extraction, injects recombined human thrombopoietin injection to donor；Anti-coagulants and inhibitor are added in syringe, extracts blood；It at 4 DEG C, is centrifuged, layering；1-3mm red blood cell under the serum and platelet rich plasma layer and platelet rich plasma layer in the sheath body handled well is moved on in another sheath body with syringe, is mixed, it is static；For suspension at 4 DEG C, centrifugation removes supernatant, and remaining is platelet rich plasma；The present invention has the characteristics that extraction platelet rich plasma speed is fast, capacity is big, with high purity, activity is high.
Description
Technical field
The present invention relates to the field of medical instrument technology, in particular to a kind of platelet rich plasma extraction element and its method.
Background technique
Platelet rich plasma (Platelet-rich plasma, PRP) is to obtain the whole blood of animal or people after centrifugation
The blood plasma rich in high concentration blood platelet arrived can be changed to jelly, therefore also referred to as Fu Xue little after fibrin ferment is added wherein
Plate gel or rich platelet leucocyte gel (PLG).Hood in 1993 etc. proposes platelet rich plasma (PRP) concept first, and
It was found that PRP blood platelet rich in, 3 times higher than number in whole blood of number or more.In blood platelet containing a large amount of growth because
Son, such as platelet derived growth factor (PDGF), transforming growth factor-β (TGF-β), insulin-like growth factor (IGF), table
Skin growth factor (EGF), vascular endothelial growth factor (VEGF) etc..In the department of stomatology and field of orthopaedics, platelet rich plasma research
It is awfully hot.Blood platelet has a big chunk to be activated to platelet rich plasma (PRP) on the market during the extraction process now, is infusing
After being mapped to disease sites, whole effects cannot be played.
Existing publication: a kind of rich platelet enriching apparatus provided by CN207707921U, the device are small size
Sampled plasma device, structure is simple, and it is convenient to operate, and is a kind of a small amount of platelet rich plasma receipts that can be ensured clinical safety and use
Collection, save set, and be conducive to surgery and realize self timely re-injection, but when platelet rich plasma extracts, speed is slow, body
Product is few, and the capacity once extracted is limited.
The blood plasma in different sources has slight difference at the extraction, and existing extraction element function is too single,
It cannot be applicable in the blood plasma of separate sources, for this purpose, it is proposed that a kind of platelet rich plasma extraction element.
Summary of the invention
The purpose of the present invention is to provide a kind of platelet rich plasma extraction element and its methods, to solve above-mentioned background skill
The problem of being proposed in art.
To achieve the above object, the invention provides the following technical scheme: a kind of platelet rich plasma extraction element, including case
Body and control system, it is characterised in that: supporting table and equipment box, fixed peace in the supporting table are fixedly connected in the cabinet
Equipped with driving motor, the output shaft of the driving motor passes through the fixed company of bottom center of supporting table and the pallet above it
It connects, the tray top is equidistant angularly to offer embedded hole, embeddable sheath body in the embedded hole；The equipment upper box part
Vibrating disk is installed, vibration pan bottom is connected with push rod, and the other end of push rod is connected with driving device, driving device driving push rod
It moves horizontally, drives sliding slot sliding of the vibrating disk on equipment box；The control system can control driving motor and driving device.
Further, it is respectively connected with lid eaves at the top of the sheath body, the sheath body outer wall is symmetrically fixedly connected with fixture block, described
Tray top, which is located at the top of embedded hole, offers card slot, and the fixture block is mutually clamped with corresponding card slot.
Further, the top of the box side is rotatably connected to case lid by hinge.
Further, the equipment box top side, which is placed, is equipped with incubator.
Further, the driving device includes servo motor, rotating plate and connecting rod, and the servo motor is fixedly mounted on
In equipment box, the output end of servo motor is fixedly connected to be parallel to each other and out-of-alignment rotating plate there are two identical, described two
The one end of rotating plate far from servo motor is articulated and connected the both ends of connecting rod by shaft respectively, is had among connecting rod rectangular logical
Hole, the push rod are equipped with push rod end, the fixed rectangle penetrated among connecting rod in push rod end close to one end of servo motor
Through-hole, servo motor drive two rotating plate vertical rotatings, and two rotating plates drive connecting rod to move up and down by shaft, connecting rod band
Dynamic push rod moves horizontally.
Further, support rod is installed, the support bar top is fixedly connected with sleeve, the set in the equipment box
Level slidably connects push rod in cylinder.
Further, the push rod end be fixed with nut insertion connecting rod among rectangular through holes, push rod it is another
End is fixedly connected with draw runner, is fixedly connected at the top of the draw runner with the vibrating disk outside equipment box, equidistantly opens up at the top of the vibrating disk
It is put into hole.
A kind of extracting method using a kind of platelet rich plasma extraction element described above, includes the following steps,
(1) before sample extraction, recombined human thrombopoietin injection is injected to donor, it is limited using Shenyang three lives pharmacy
The spy of responsible company's production is than Australia's recombined human thrombopoietin injection；
(2) anti-coagulants sodium citrate and prostaglandin E1 inhibitor are added in syringe, extracts blood, in which: anti-coagulants
It is 1:8-10 with blood ratios, inhibitor and blood ratios are 1:5-7；
(3) the blood 500ml-800ml that step (2) is handled well is uniformly distributed into sheath body first, covers lid eaves 22, it will
The good sheath body of fixture block card, is put into the embedded hole of pallet, and sheath body and card slot coincide, and at 4 DEG C, is centrifuged 10min ± 1min, in sheath body
Whole blood be divided into 3 layers, upper layer is serum, and lower layer is red blood cell, the pale yellow chromatograph that middle layer is one layer very thin, i.e. rich platelet blood
Pulp layer；
(4) 1- under the serum and PRP layers and platelet rich plasma layer in sheath body handled well step (3) with syringe
3mm red blood cell moves on in another sheath body, mixes, at 4 DEG C, static 20-40min；
(5) the suspension 500ml-800ml for handling step (4) well, is uniformly distributed into sheath body, covers lid eaves, by fixture block
Sheath body is blocked, has been put into the embedded hole 5 of pallet 3, sheath body and card slot coincide, and at 4 DEG C, are centrifuged 10min ± 1min, supernatant is moved
It removes, remaining is platelet rich plasma.
Further, after being handled by above step (1)-(5), blood is again split into three layers, and upper layer is plasma layer, centre
Layer be include blood platelet, the tunica albuginea layer of white blood cell, lower layer be red blood cell layer, sheath body is removed, the syringe needle of needle tubing is inserted into blood plasma
In blood platelet between white blood cell, thrombocyte plasma is extracted out, calcium or fibrin ferment are added in the blood platelet of extraction, makes blood platelet
Activation can be injected into skin corium, convenient to extract to thrombocyte plasma.
Compared with prior art, the beneficial effects of the present invention are:
The present invention has the characteristics that extraction platelet rich plasma speed is fast, capacity is big, activity is high.
1, the platelet rich plasma activity that the present invention extracts is high, in the extraction process of platelet rich plasma, in addition to being added
Anti-coagulants is additionally added inhibitor, and the work of bactericidal antiphlogistic more can be enhanced in the effective activation for inhibiting blood platelet when for wound
With.
2, the platelet rich plasma speed that the present invention extracts is fast, capacity is big, and tray design is reasonable, can handle simultaneously a large amount of
Blood, safe and reliable to operation, centrifugation apparatus design rationally, at the top of sheath body is respectively connected with lid eaves, meanwhile, fixture block and card slot kissing
It closes, when driving motor carries out telling rotation, good separating effect is not easy to leak outside；Vibrating disc is ingenious in design, can shake separation simultaneously
The blood plasma of a large amount of separate sources, good separating effect.
Detailed description of the invention
Fig. 1 is overall structure cross-sectional view of the present invention；
Fig. 2 is support holder structure top view of the present invention；
Fig. 3 is the A-A cross-sectional view of Fig. 2；
Fig. 4 is vibrating tray structure top view of the present invention.
In figure: 1, cabinet；2, supporting table；3, pallet；4, driving motor；5, embedded hole；6, equipment box；7, sheath body；8, block
Block；9, card slot；10, rotating plate；11, servo motor；111, shaft；12, connecting rod；13, push rod；131, push rod end；14, it supports
Bar；15, sleeve；16, draw runner；17, vibrating disk；18, it is put into hole；19, sliding slot；20, case lid；21, incubator；22, lid eaves.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
A kind of platelet rich plasma extraction element, including cabinet 1 and control system, it is characterised in that: solid in the cabinet 1
Surely it is connected with supporting table 2 and equipment box 6, driving motor 4, the output of the driving motor 4 are fixedly installed in the supporting table 2
Axis passes through supporting table 2 and is fixedly connected with the bottom center of the pallet 3 above it, and 3 top of pallet is equidistant angularly to open
Equipped with embedded hole 5, embeddable sheath body 7 in the embedded hole 5；6 top of equipment box is equipped with vibrating disk 17,17 bottom of vibrating disk
Portion is connected with push rod 13, and the other end of push rod is connected with driving device, and driving device driving push rod 13 moves horizontally, and drives vibration
Sliding slot 19 of the disk 17 on equipment box 6 slides；The control system can control driving motor 4 and driving device.
Further, it is respectively connected with lid eaves 22 at the top of the sheath body 7, safe and reliable to operation, blood can not leak outside, the set
7 outer wall of shell is symmetrically fixedly connected with fixture block 8, is located at the top of embedded hole 5 at the top of the pallet 3 and offers card slot 9, the fixture block 8
It is clamped with corresponding 9 phase of card slot.
Further, 1 top side of cabinet is rotatably connected to case lid 20 by hinge.
Further, 6 top side of equipment box, which is placed, is equipped with incubator 21.
Further, the driving device includes servo motor 11, rotating plate 10 and connecting rod 12, and the servo motor 11 is solid
In the equipment box 6, the output end of servo motor 11 is fixedly connected to be parallel to each other and out-of-alignment turns there are two identical for Dingan County
Plate 10, the described two one end of rotating plate 10 far from servo motor 11 pass through the both ends of the articulated connection connecting rod 12 of shaft 111 respectively,
There is rectangular through-hole among connecting rod 12, the push rod 13 is equipped with push rod end 131, push rod close to one end of servo motor 11
The fixed rectangular through holes penetrated among connecting rod 12 in end 131, two 10 vertical rotatings of rotating plate of drive of servo motor 11, two
Rotating plate 10 drives connecting rod 12 to move up and down by shaft 111, and connecting rod 12 drives push rod 13 to move horizontally.
Further, support rod 14 is installed in the equipment box 6, is fixedly connected with sleeve at the top of the support rod 14
15, level slidably connects push rod 13 in the sleeve 15.
Further, the push rod end 131 is fixed with the rectangular through holes that nut penetrates 12 centre of connecting rod, push rod 13
The other end be fixedly connected with draw runner 16,16 top of draw runner is fixedly connected with the vibrating disk 17 outside equipment box 6, the vibrating disk
17 tops, which equidistantly offer, is put into hole 18.
Working principle are as follows: the sheath body 7 equipped with blood is put into embedded hole 5, the sheath body 7 of different-diameter matches different cards
Block 8 clamps fixture block 8 just by sheath body 7, meanwhile, fixture block 8 matches with card slot 9, and when driving motor 4 rotates, pallet 3 is opened
Begin quickly to rotate, be separated blood plasma, blood platelet, white blood cell and the red blood cell in blood by centrifugal force, by sheath body after separation
It removes, the syringe needle of needle tubing is inserted into the blood platelet between blood plasma and white blood cell, thrombocyte plasma is extracted out, in the blood platelet of extraction
In calcium or fibrin ferment is added, make biologically active pdgf, skin corium can be injected into, it is convenient that thrombocyte plasma is extracted,
Convenient for the timely injection of patient, the activity of blood platelet is kept.
Sheath body 7 containing blood is placed on being put into hole 18 of vibrating disk 17, passes through the rotation of servo motor 11, drives two
A 10 vertical rotating of rotating plate, two rotating plates drive connecting rod 12 to move up and down by shaft 111, and connecting rod 12 drives push rod 13 to exist
Horizontal round-trip sliding, push rod 13 push 16 back and forth movement of draw runner to make blood so that vibrating disk 17 be made ceaselessly to vibrate in sleeve 15
In different specific weight ingredient between it is disconnected from each other, by control servo motor 11 revolving speed can control different vibration frequencies with
Just it is suitable for the blood plasma of separate sources.
Embodiment 1
A kind of extracting method using a kind of platelet rich plasma extraction element described above, includes the following steps,
(1) before sample extraction, recombined human thrombopoietin injection is injected to donor, it is limited using Shenyang three lives pharmacy
The spy of responsible company's production is than Australia's recombined human thrombopoietin injection；
(2) sodium citrate anticoagulant and 16ml prostaglandin E1 inhibitor of 10ml are added in syringe, extracts blood
80ml；
(3) the blood 500ml that step (2) is handled well is uniformly distributed into sheath body first, covers lid eaves 22, by fixture block card
Good sheath body, is put into the embedded hole of pallet, and sheath body and card slot coincide, and at 4 DEG C, is centrifuged 9min, the whole blood in sheath body is divided into 3
Layer, upper layer are serum, and lower layer is red blood cell, the pale yellow chromatograph that middle layer is one layer very thin, i.e. platelet rich plasma layer；
(4) serum in sheath body and platelet rich plasma layer and rich platelet handled well step (3) with syringe
1-3mm red blood cell moves on in another sheath body under plasma layer, mixes, at 4 DEG C, static 20min；
(5) the suspension 500ml for handling step (4) well, is uniformly distributed into sheath body, covers lid eaves, and fixture block card is covered well
Shell is put into the embedded hole 5 of pallet 3, and sheath body and card slot coincide, and at 4 DEG C, is centrifuged 9min, supernatant is removed, remaining to be
Platelet rich plasma.
Further, after being handled by above step (1)-(5), blood is again split into three layers, and upper layer is plasma layer, centre
Layer be include blood platelet, the tunica albuginea layer of white blood cell, lower layer be red blood cell layer, sheath body is removed, the syringe needle of needle tubing is inserted into blood plasma
In blood platelet between white blood cell, thrombocyte plasma is extracted out, calcium or fibrin ferment are added in the blood platelet of extraction, makes blood platelet
Activation can be injected into skin corium, convenient to extract to thrombocyte plasma.
Embodiment 2
A kind of extracting method using a kind of platelet rich plasma extraction element described above, includes the following steps,
(1) before sample extraction, recombined human thrombopoietin injection is injected to donor, it is limited using Shenyang three lives pharmacy
The spy of responsible company's production is than Australia's recombined human thrombopoietin injection；
(2) sodium citrate anticoagulant and 15ml prostaglandin E1 inhibitor of 10ml are added in syringe, extracts blood
90ml；
(3) the blood 600g that step (2) is handled well is uniformly distributed into sheath body first, covers lid eaves 22, by fixture block card
Good sheath body, is put into the embedded hole of pallet, and sheath body and card slot coincide, and at 4 DEG C, is centrifuged 10min, the whole blood in sheath body is divided into 3
Layer, upper layer are serum, and lower layer is red blood cell, the pale yellow chromatograph that middle layer is one layer very thin, i.e. PRP layers；
(4) serum and PRP layers and PRP layers lower 1-3mm red blood cell in sheath body handled well step (3) with syringe
It moves on in another sheath body, mixes, at 4 DEG C, static 30min；
(5) the suspension 600ml for handling step (4) well, is uniformly distributed into sheath body, covers lid eaves, and fixture block card is covered well
Shell is put into the embedded hole 5 of pallet 3, and sheath body and card slot coincide, and at 4 DEG C, is centrifuged 10min, supernatant is removed, remaining to be
Platelet rich plasma.
Further, after being handled by above step (1)-(5), blood is again split into three layers, and upper layer is plasma layer, centre
Layer be include blood platelet, the tunica albuginea layer of white blood cell, lower layer be red blood cell layer, sheath body is removed, the syringe needle of needle tubing is inserted into blood plasma
In blood platelet between white blood cell, thrombocyte plasma is extracted out, calcium or fibrin ferment are added in the blood platelet of extraction, makes blood platelet
Activation can be injected into skin corium, convenient to extract to thrombocyte plasma.
Embodiment 3
A kind of extracting method using a kind of platelet rich plasma extraction element described above, includes the following steps,
(1) before sample extraction, recombined human thrombopoietin injection is injected to donor, it is limited using Shenyang three lives pharmacy
The spy of responsible company's production is than Australia's recombined human thrombopoietin injection；
(2) sodium citrate anticoagulant and 15ml prostaglandin E1 inhibitor of 10ml are added in syringe, extracts blood
100ml；
(3) the blood 800ml that step (2) is handled well is uniformly distributed into sheath body first, covers lid eaves 22, by fixture block card
Good sheath body, is put into the embedded hole of pallet, and sheath body and card slot coincide, and at 4 DEG C, is centrifuged 11min, the whole blood in sheath body is divided into 3
Layer, upper layer are serum, and lower layer is red blood cell, the pale yellow chromatograph that middle layer is one layer very thin, i.e. platelet rich plasma layer；
(4) serum in sheath body and platelet rich plasma layer and rich platelet handled well step (3) with syringe
1-3mm red blood cell moves on in another sheath body under plasma layer, mixes, at 4 DEG C, static 30min；
(5) the suspension 800ml for handling step (4) well, is uniformly distributed into sheath body, covers lid eaves, and fixture block card is covered well
Shell is put into the embedded hole 5 of pallet 3, and sheath body and card slot coincide, and at 4 DEG C, is centrifuged 11min, supernatant is removed, remaining to be
Platelet rich plasma.
Further, after being handled by above step (1)-(5), blood is again split into three layers, and upper layer is plasma layer, centre
Layer be include blood platelet, the tunica albuginea layer of white blood cell, lower layer be red blood cell layer, sheath body is removed, the syringe needle of needle tubing is inserted into blood plasma
In blood platelet between white blood cell, thrombocyte plasma is extracted out, calcium or fibrin ferment are added in the blood platelet of extraction, makes blood platelet
Activation can be injected into skin corium, convenient to extract to thrombocyte plasma.
Embodiment 4
A kind of extracting method using a kind of platelet rich plasma extraction element described above, includes the following steps,
(1) (limited using the pharmacy of the Shenyang three lives to donor injection recombined human thrombopoietin injection before sample extraction
The spy of responsible company's production is than Australia's recombined human thrombopoietin injection)；
(2) sodium citrate anticoagulant and 15ml prostaglandin E1 inhibitor of 11ml are added in syringe, extracts blood
105ml；
(3) the blood 700ml that step (2) is handled well is uniformly distributed into sheath body first, covers lid eaves 22, by fixture block card
Good sheath body, is put into the embedded hole of pallet, and sheath body and card slot coincide, and at 4 DEG C, is centrifuged 10min, the whole blood in sheath body is divided into 3
Layer, upper layer are serum, and lower layer is red blood cell, the pale yellow chromatograph that middle layer is one layer very thin, i.e. platelet rich plasma layer；
(4) serum in sheath body and platelet rich plasma layer and rich platelet handled well step (3) with syringe
1-3mm red blood cell moves on in another sheath body under plasma layer, mixes, at 4 DEG C, static 40min；
(5) the suspension 700ml for handling step (4) well, is uniformly distributed into sheath body, covers lid eaves, and fixture block card is covered well
Shell is put into the embedded hole 5 of pallet 3, and sheath body and card slot coincide, and at 4 DEG C, is centrifuged 11min, supernatant is removed, remaining to be
Platelet rich plasma.
Further, after being handled by above step (1)-(5), blood is again split into three layers, and upper layer is plasma layer, centre
Layer be include blood platelet, the tunica albuginea layer of white blood cell, lower layer be red blood cell layer, sheath body is removed, the syringe needle of needle tubing is inserted into blood plasma
In blood platelet between white blood cell, thrombocyte plasma is extracted out, calcium or fibrin ferment are added in the blood platelet of extraction, makes blood platelet
Activation can be injected into skin corium, convenient to extract to thrombocyte plasma.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (9)
1. a kind of platelet rich plasma extraction element, including cabinet (1) and control system, it is characterised in that: in the cabinet (1)
It is fixedly connected with supporting table (2) and equipment box (6), is fixedly installed with driving motor (4), the driving in the supporting table (2)
The output shaft of motor (4) passes through supporting table (2) and is fixedly connected with the bottom center of the pallet (3) above it, the pallet (3)
Top is equidistant angularly to be offered embedded hole (5), embeddable sheath body (7) in the embedded hole (5)；On the equipment box (6)
Portion is equipped with vibrating disk (17), and vibrating disk (17) bottom is connected with push rod (13), and the other end of push rod is connected with driving device, is driven
Dynamic device driving push rod (13) move horizontally, and drive sliding slot (19) sliding of vibrating disk (17) on equipment box (6)；The control
System can control driving motor (4) and driving device.
2. a kind of platelet rich plasma extraction element according to claim 1, it is characterised in that: at the top of the sheath body (7)
It is respectively connected with lid eaves (22), sheath body (7) outer wall is symmetrically fixedly connected with fixture block (8), and insertion is located at the top of the pallet (3)
It is offered card slot (9) at the top of hole (5), the fixture block (8) is mutually clamped with corresponding card slot (9).
3. a kind of platelet rich plasma extraction element according to claim 1, it is characterised in that: at the top of the cabinet (1)
Side is rotatably connected to case lid (20) by hinge.
4. a kind of platelet rich plasma extraction element according to claim 1, it is characterised in that: equipment box (6) top
Portion side, which is placed, is equipped with incubator (21).
5. a kind of platelet rich plasma extraction element according to claim 1, it is characterised in that: the driving device includes
Servo motor (11), rotating plate (10) and connecting rod (12), the servo motor (11) are fixedly mounted in equipment box (6), servo
The output end of motor (11) is fixedly connected to be parallel to each other and out-of-alignment rotating plate (10) there are two identical, described two rotating plates
(10) one end far from servo motor (11) passes through the both ends that shaft (111) is hingedly connected to connecting rod (12), connecting rod respectively
(12) intermediate to have rectangular through holes, the push rod (13) is equipped with push rod end (131) close to the one end of servo motor (11), push rod
End (131) is fixed to penetrate the intermediate rectangular through holes of connecting rod (12), and servo motor (11) drives two rotating plates (10) vertical
Rotation, two rotating plates (10) drive connecting rod (12) to move up and down by shaft (111), and connecting rod (12) drives push rod (13) water
Translation is dynamic.
6. a kind of platelet rich plasma extraction element according to claim 5, it is characterised in that: in the equipment box (6)
It is equipped with support rod (14), is fixedly connected with sleeve (15) at the top of the support rod (14), horizontal sliding in the sleeve (15)
It is connected with push rod (13).
7. a kind of platelet rich plasma extraction element according to claim 5, it is characterised in that: the push rod end
(131) the intermediate rectangular through holes of nut insertion connecting rod (12) are fixed with, the other end of push rod (13) is fixedly connected with draw runner
(16), it is offered sliding slot (19) at the top of the equipment box (6), the draw runner (16) (19) can be slided along the chute, the draw runner
(16) top is fixedly connected with the vibrating disk (17) of equipment box (6) outside, is equidistantly offered at the top of the vibrating disk (17) and is put into hole
(18)。
8. it is a kind of using a kind of extracting method of platelet rich plasma extraction element according to any one of claims 1 to 7,
It is characterized in that, includes the following steps,
(1) before sample extraction, recombined human thrombopoietin injection is injected to donor；
(2) anti-coagulants and inhibitor are added in syringe, extracts blood, in which: anti-coagulants is 1:8-10 with blood ratios, is inhibited
Agent and blood ratios are 1:5-7；
(3) the blood 500ml-800ml that step (2) is handled well is uniformly distributed into sheath body first, covers lid eaves 22, by fixture block
Sheath body is blocked, has been put into the embedded hole of pallet, sheath body and card slot coincide, and at 4 DEG C, are centrifuged 10min ± 1min, complete in sheath body
Blood system is 3 layers, and upper layer is serum, and lower layer is red blood cell, the pale yellow chromatograph that middle layer is one layer very thin, i.e. platelet rich plasma
Layer；
(4) serum in sheath body and platelet rich plasma layer and platelet rich plasma handled well step (3) with syringe
The lower 1-3mm red blood cell of layer moves on in another sheath body, mixes, at 4 DEG C, static 20-40min；
(5) the suspension 500ml-800ml for handling step (4) well, is uniformly distributed into sheath body, covers lid eaves, and fixture block card is good
Sheath body is put into the embedded hole 5 of pallet 3, and sheath body and card slot coincide, and at 4 DEG C, is centrifuged 10min ± 1min, supernatant is removed,
Remaining is platelet rich plasma.
9. a kind of extracting method of platelet rich plasma extraction element according to claim 8, it is characterised in that: pass through step
Suddenly after (1)-(5) processing, blood is again split into three layers, and upper layer is plasma layer, middle layer is the tunica albuginea for including blood platelet, white blood cell
Layer, lower layer are red blood cell layer, and sheath body is removed, and the syringe needle of needle tubing are inserted into the blood platelet between blood plasma and white blood cell, extraction
Calcium or fibrin ferment are added in the blood platelet of extraction, makes biologically active pdgf, can be injected into skin corium for thrombocyte plasma,
It is convenient that thrombocyte plasma is extracted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811594893.1A CN109692761A (en) | 2018-12-25 | 2018-12-25 | A kind of platelet rich plasma extraction element and its method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811594893.1A CN109692761A (en) | 2018-12-25 | 2018-12-25 | A kind of platelet rich plasma extraction element and its method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109692761A true CN109692761A (en) | 2019-04-30 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113499609A (en) * | 2021-08-19 | 2021-10-15 | 拜澳泰克(沈阳)生物医学集团有限公司 | Platelet-rich plasma automatic extraction device |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3720368A (en) * | 1971-07-15 | 1973-03-13 | Bio Dynamics Inc | Centrifuge with blood sample holding means |
| DE3101078A1 (en) * | 1980-01-16 | 1982-02-18 | Separex Teknik AB, 11633 Stockholm | Centrifuge |
| CN1503698A (en) * | 2001-04-20 | 2004-06-09 | 日立工机株式会社 | Centrifugal machine |
| CN102665922A (en) * | 2009-11-11 | 2012-09-12 | 费柏莱特离心机有限公司 | Fixed angle centrifuge rotor with tubular cavities and related methods |
| CN104383726A (en) * | 2012-07-30 | 2015-03-04 | 博雅干细胞科技有限公司 | Extraction method of platelet rich plasma and extracted platelet rich plasma |
| CN204233506U (en) * | 2014-11-19 | 2015-04-01 | 沈阳优吉诺生物科技有限公司 | A kind of test kit of rapid extraction blood plasma |
| CN106727700A (en) * | 2016-11-29 | 2017-05-31 | 第五空间健康管理江苏有限公司 | Prepare the method for platelet rich plasma PRP and the purposes of the platelet rich plasma |
| CN107198893A (en) * | 2017-07-01 | 2017-09-26 | 张方亮 | Leucocyte-removing PRP preparation method |
| CN108479177A (en) * | 2018-06-06 | 2018-09-04 | 郑州大学第附属医院 | A kind of separator for extracting blood platelet from whole blood |
| CN108593408A (en) * | 2018-05-04 | 2018-09-28 | 罗继伟 | A kind of anti-condensation rocking equipment of blood testing blood |
-
2018
- 2018-12-25 CN CN201811594893.1A patent/CN109692761A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3720368A (en) * | 1971-07-15 | 1973-03-13 | Bio Dynamics Inc | Centrifuge with blood sample holding means |
| DE3101078A1 (en) * | 1980-01-16 | 1982-02-18 | Separex Teknik AB, 11633 Stockholm | Centrifuge |
| CN1503698A (en) * | 2001-04-20 | 2004-06-09 | 日立工机株式会社 | Centrifugal machine |
| CN102665922A (en) * | 2009-11-11 | 2012-09-12 | 费柏莱特离心机有限公司 | Fixed angle centrifuge rotor with tubular cavities and related methods |
| CN104383726A (en) * | 2012-07-30 | 2015-03-04 | 博雅干细胞科技有限公司 | Extraction method of platelet rich plasma and extracted platelet rich plasma |
| CN204233506U (en) * | 2014-11-19 | 2015-04-01 | 沈阳优吉诺生物科技有限公司 | A kind of test kit of rapid extraction blood plasma |
| CN106727700A (en) * | 2016-11-29 | 2017-05-31 | 第五空间健康管理江苏有限公司 | Prepare the method for platelet rich plasma PRP and the purposes of the platelet rich plasma |
| CN107198893A (en) * | 2017-07-01 | 2017-09-26 | 张方亮 | Leucocyte-removing PRP preparation method |
| CN108593408A (en) * | 2018-05-04 | 2018-09-28 | 罗继伟 | A kind of anti-condensation rocking equipment of blood testing blood |
| CN108479177A (en) * | 2018-06-06 | 2018-09-04 | 郑州大学第附属医院 | A kind of separator for extracting blood platelet from whole blood |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113499609A (en) * | 2021-08-19 | 2021-10-15 | 拜澳泰克(沈阳)生物医学集团有限公司 | Platelet-rich plasma automatic extraction device |
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