CN113499609A - Platelet-rich plasma automatic extraction device - Google Patents
Platelet-rich plasma automatic extraction device Download PDFInfo
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- CN113499609A CN113499609A CN202110955130.0A CN202110955130A CN113499609A CN 113499609 A CN113499609 A CN 113499609A CN 202110955130 A CN202110955130 A CN 202110955130A CN 113499609 A CN113499609 A CN 113499609A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/12—Auxiliary equipment particularly adapted for use with liquid-separating apparatus, e.g. control circuits
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
- B04B11/06—Arrangement of distributors or collectors in centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
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- Chemical & Material Sciences (AREA)
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Abstract
The invention relates to the field of medical instruments, in particular to an automatic platelet-rich plasma extraction device which comprises an aluminum profile combined frame, a centrifugal mechanism, a centrifuge tube carrying mechanism and an auxiliary liquid transfer mechanism. The device provided by the invention can realize the full automation of the process from centrifugation to PRP extraction, is simple to operate, saves manpower and has high extraction efficiency; the prepared product contains more platelets with higher concentration, and the process that the plasma is exposed in the air is avoided in the extraction process, so that the possibility of plasma pollution is reduced, and the infection probability is reduced; the extracted PRP is directly placed in a syringe, so that the use or the storage is convenient; the discharged red blood cell plasma is directly injected into the recovery bag, so that a user can prepare the required low-concentration platelet rich plasma and high-concentration platelet rich plasma without an operating room or a sterile super clean bench, and the defects of long preparation period, easy infection, low platelet concentration, non-universal centrifugal equipment and limited manufacturing place of other methods are overcome.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to an automatic platelet-rich plasma extraction device.
Background
Platelet-rich plasma (PRP) is Platelet-rich plasma obtained by centrifuging whole blood of animals or humans. When activated, platelets release a variety of growth factors that play important roles in promoting the proliferation, growth, differentiation of osteocytes and osteoblasts, and tissue formation. Since Marx et al used PRP composite bone graft to repair mandibular defect for the first time in 1998, PRP has been gradually applied to tissue repair in the fields of oral cavity, plastic surgery, orthopaedics, otorhinolaryngology, neurosurgery and the like, and also used in the fields of medical cosmetology, alopecia treatment, aging resistance and the like.
In the prior art, the extraction and preparation method of PRP does not form a unified standard, and the preparation method mainly comprises 2 methods of a density gradient centrifugation method and a plasma separation and replacement method. The density gradient centrifugation method is to separate and extract PRP from whole blood according to different sedimentation coefficients of components in the blood, and a secondary centrifugation method is generally adopted at present, however, the concentration and activity of platelets in PRP prepared by different centrifugal forces and centrifugal time are different, cell components in the blood are removed in the operation process, the operation is complex, the pollution is easily caused, the enrichment speed of platelet-rich plasma is low, and the clinical application of the platelet-rich plasma is limited; the plasma separation and replacement method is mainly applied to the collection of blood platelets in a blood bank and the transfusion of clinical components.
Disclosure of Invention
In order to overcome the above defects of the prior art, the present invention aims to provide an automatic platelet-rich plasma extraction device, which aims to solve the technical problems that: how to collect platelet-rich plasma from centrifugation to PRP extraction is inefficient.
In order to achieve the above object, the present invention provides the following technical solutions.
An automatic platelet-rich plasma extraction device comprises an aluminum profile combined frame, a centrifugal mechanism, a centrifuge tube carrying mechanism and an auxiliary liquid-transferring mechanism, the bottom end of the aluminum profile combined frame is fixedly provided with a centrifugal mechanism mounting plate, the side wall and the rear wall of the aluminum profile combined frame are respectively fixedly provided with a combined centrifugal injector placing safety door and a PRP extraction injector taking safety door, the middle part of the aluminum profile combined frame is fixedly provided with an equipment workbench, the aluminum profile combined frame is divided into an upper layer and a lower layer by the equipment workbench, the centrifugal mechanism is arranged on the lower layer, the centrifuge tube carrying mechanism and the auxiliary liquid transferring mechanism are arranged on the upper layer, the centrifugal mechanism is fixedly connected with the aluminum profile combined frame through a centrifugal mechanism mounting plate, supplementary liquid mechanism that moves passes through equipment workstation and aluminium alloy composite frame fixed connection, centrifuging tube transport mechanism is fixed to be set up in aluminium alloy composite frame upper rear side.
The centrifugal mechanism consists of a variable frequency motor, a speed reducer, a synchronous belt and gear combined speed change mechanism mounting plate, a synchronous belt and gear combined speed change mechanism, a centrifugal mechanism rotating main shaft, a combined centrifugal injector rotating disc and a combined centrifugal injector gravity overturning mechanism, wherein the variable frequency motor is fixedly connected with the speed reducer, the speed reducer is mounted on the right side of the upper surface of the centrifugal mechanism mounting plate, the synchronous belt and gear combined speed change mechanism mounting plate fixedly connected with the centrifugal mechanism mounting plate is mounted on the upper surface of the speed reducer, the synchronous belt and gear combined speed change mechanism coupled with an output shaft of the speed reducer are fixedly mounted right above the synchronous belt and gear combined speed change mechanism mounting plate, the centrifugal mechanism rotating main shaft is the tail end output shaft section of the synchronous belt and gear combined speed change mechanism, and passes through an equipment workbench to be coupled with the combined centrifugal injector rotating disc, the combined centrifugal injector rotating disc is arranged in a V shape, the two sides of the combined centrifugal injector rotating disc are respectively provided with a combined centrifugal injector gravity turnover mechanism, and a combined centrifugal injector and a counterweight combined pipe are respectively arranged in the two combined centrifugal injector gravity turnover mechanisms.
The auxiliary pipetting mechanism is composed of a centrifuge tube auxiliary disassembly pipetting frame, a PRP extraction syringe, an auxiliary pipetting mechanism mounting plate, a pipetting frame Z, a motor lead screw linear module, a combined centrifuge tube disassembly auxiliary clamping jaw, an auxiliary pipetting mechanism X, a servo rotary disk, an injection end sealing clamping jaw, a pipette connector clamping jaw, a liquid separation pipette, a red blood cell plasma recovery bag, a PRP extraction syringe fixing frame, a first liquid separation pipette sealing clamping jaw and a second liquid separation pipette sealing clamping jaw, the auxiliary pipetting mechanism is arranged on the right side of the upper surface of the equipment workbench through the auxiliary pipetting mechanism mounting plate, the centrifuge tube auxiliary disassembly pipetting frame and the auxiliary pipetting mechanism X are arranged on the auxiliary pipetting mechanism mounting plate to the motor lead screw linear module, the auxiliary pipetting mechanism X is positioned right in front of the centrifuge tube auxiliary disassembly pipetting frame, install on the supplementary liquid moving frame Z of dismantling of centrifuging tube and to motor lead screw straight line module, liquid moving frame Z installs combination centrifuging tube dismantles supplementary clamping jaw on to the slip table of motor lead screw straight line module, installs two servo rotary disks on the slip table of supplementary liquid moving mechanism X to motor lead screw straight line module, installs injection end closing cap clamping jaw and pipette connector clamping jaw respectively on two servo rotary disks, has placed the liquid separating pipette in the clamping jaw gap of pipette connector clamping jaw.
In a preferred embodiment, the centrifuge tube carrying mechanism comprises an X-direction motor screw linear transmission module, a centrifuge tube carrying and pipetting auxiliary gripper, a Z-direction motor screw linear transmission module, an electric rotating spindle mounting rack, a four-jaw chuck type pneumatic finger, a push rod gripping jaw mounting rack and a push rod gripping jaw.
In a preferred embodiment, X is to the top of motor lead screw linear transmission module setting at the equipment workstation, install on aluminium alloy composite frame's both sides stand, install centrifuging tube transport and move liquid supplementary tongs on X is to the slip table of motor lead screw linear transmission module, centrifuging tube transport and move liquid supplementary tongs through electric rotating spindle mounting bracket and X to the slip table fixed connection of motor lead screw linear transmission module, install perpendicular decurrent electric rotating spindle above electric rotating spindle mounting bracket, the output shaft and the pneumatic finger fixed coupling of four-jaw chuck formula of electric rotating spindle, it snatchs the clamping jaw mounting bracket to install the push rod in one side of electric rotating spindle mounting bracket, the push rod is installed the push rod and is snatched the clamping jaw at the bottom end that the clamping jaw mounting bracket was snatched to the push rod.
In a preferred embodiment, the liquid separating pipette is integrally made and consists of five parts, namely a red blood cell plasma recovery bag, a combined centrifugal syringe connector, a PRP extraction syringe connector, a first branch pipe and a second branch pipe.
In a preferred embodiment, divide the liquid pipette and the fixed combination centrifugal syringe connector that is equipped with of combination centrifugal syringe connector, divide the one end dress card that the liquid pipette has the combination centrifugal syringe connector at pipette connector clamping jaw positive center, divide liquid pipette both ends branch pipe to pass first branch liquid pipette and seal the clamping jaw with second branch liquid pipette respectively, divide the liquid pipette and draw the fixed PRP that is equipped with of syringe connector with PRP and draw the syringe connector, it is fixed with PRP and draw syringe mount dress card to have the one end that PRP drawed the syringe connector to pass second branch liquid pipette and seal the clamping jaw, and the one end that the liquid pipette has red cell plasma to retrieve the bag passes first branch liquid pipette and seals the clamping jaw, and the red cell plasma is retrieved the bag and is placed in the bottom space of first branch liquid pipette seal clamping jaw.
In a preferred embodiment, the combined centrifugal injector and the counterweight combined tube have the same structure and are composed of a combined centrifugal tube liquid storage tube, an injection end mounting turnbuckle, a push rod end mounting turnbuckle, an injector push rod, a push rod limiting sleeve, a sleeve turnbuckle connecting groove, an injection end sealing cover and a sealing cover turnbuckle connecting groove, and the combined centrifugal injector is divided into two working forms, namely an injector form and a centrifugal tube form.
In a preferred embodiment, the syringe configuration: is a basic form of a combined centrifugal syringe and is used for extracting blood plasma and consists of a combined centrifugal tube liquid storage tube, a syringe push rod and a corresponding needle head.
In a preferred embodiment, the centrifuge tube configuration of the combination centrifugal syringe is: after the plasma extraction finishes, take off the syringe needle, make injection end closing cap fixed mounting on combination centrifuging tube deposits the liquid pipe through the buckle notch structure of injection end installation turnbuckle and closing cap turnbuckle spread groove, make the spacing pipe sleeve fixed mounting of push rod on combination centrifuging tube deposits the liquid pipe through the buckle notch structure of push rod end installation turnbuckle and pipe sleeve turnbuckle spread groove for place and carry out centrifugal operation in centrifugal mechanism.
Compared with the prior art, the invention has the following technical effects and advantages.
The invention can realize the full automation of the process from centrifugation to PRP extraction, has simple operation, saves manpower and has high extraction efficiency; the prepared product contains more platelets with higher concentration, and the process that the plasma is exposed in the air is avoided in the extraction process, so that the possibility of plasma pollution is reduced, and the infection probability is reduced; the extracted PRP is directly placed in a syringe, so that the use or the storage is convenient; the discharged red blood cell plasma is directly injected into the recovery bag, so that a user can prepare the required low-concentration platelet rich plasma and high-concentration platelet rich plasma without an operating room or a sterile super clean bench, and the defects of long preparation period, easy infection, low platelet concentration, non-universal centrifugal equipment and limited manufacturing place of other methods are overcome.
Drawings
Fig. 1 is a schematic view of the overall three-dimensional structure of the present invention.
Fig. 2 is a front view of the overall structure of the present invention.
FIG. 3 is a three-dimensional schematic view of a centrifugal mechanism according to the present invention.
FIG. 4 is a three-dimensional schematic view of a centrifuge tube handling mechanism of the present invention.
Fig. 5 is a first perspective structural diagram of the auxiliary pipetting mechanism of the present invention.
Fig. 6 is a second perspective structural view of the auxiliary pipetting mechanism of the present invention.
Fig. 7 is a schematic view of a connection of a dispensing pipette of the present invention.
FIG. 8 is a schematic view of the combined centrifugal injector syringe of the present invention in a state.
Fig. 9 is a schematic view of the centrifuge tube state of the combined centrifugal syringe of the present invention.
The reference signs are: 1 aluminum profile combined frame, 2 centrifugal mechanisms, 3 combined centrifugal injectors, 4 counterweight combined tubes, 5 centrifugal tube carrying mechanisms, 6 centrifugal tube auxiliary disassembly liquid transferring frames, 7 auxiliary liquid transferring mechanisms, 8 PRP extraction injectors, 9 centrifugal mechanism mounting plates, 10 equipment workbenches, 11 combined centrifugal injector placing safety doors, 12 PRP extraction injector taking safety doors, 13 variable frequency motors, 14 speed reducers, 15 synchronous belt and gear combined speed change mechanism mounting plates, 16 synchronous belt and gear combined speed change mechanisms, 17 centrifugal mechanism rotating main shafts, 18 combined centrifugal injector rotating discs, 19 combined centrifugal injector gravity overturning mechanisms, 20 combined centrifugal tube liquid storing tubes, 21 injection end mounting turnbuckles, 22 push rod end mounting turnbuckles, 23 injector push rods, 24 push rod limiting pipe sleeves, 25 pipe sleeve turnbuckle connecting grooves, 26 injection end sealing covers, 27 sealing cover turnbuckle connecting grooves, 28X-direction motor screw linear transmission module, 29 centrifuge tube carrying and liquid transferring auxiliary gripper, 30Z-direction motor screw linear transmission module, 31 electric rotating main shaft, 32 electric rotating main shaft mounting rack, 33 four-jaw chuck type pneumatic finger, 34 push rod gripping gripper mounting rack, 35 push rod gripping gripper, 36 auxiliary liquid transferring mechanism mounting board, 37 liquid transferring rack Z-direction motor screw linear module, 38 combined centrifuge tube dismounting auxiliary gripper, 39 auxiliary liquid transferring mechanism X-direction motor screw linear module, 40 servo rotating disk, 41 injection end sealing cap gripper, 42 liquid transferring head gripper, 43 liquid separating pipette, 44 red blood cell plasma recovery bag, 45 PRP extraction injector mounting rack, 46 first liquid separating pipette sealing gripper, 47 second liquid separating pipette sealing gripper, 48 combined centrifugal injector connector, 49 PRP extraction injector connector, 50 first branch tube, 51 second branch.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides an automatic platelet-rich plasma extraction device, which comprises an aluminum profile combined frame 1, a centrifugal mechanism 2, a centrifuge tube carrying mechanism 5 and an auxiliary liquid-transferring mechanism 7, wherein the bottom end of the aluminum profile combined frame 1 is fixedly provided with a centrifugal mechanism mounting plate 9, the side wall and the rear wall of the aluminum profile combined frame 1 are respectively fixedly provided with a combined centrifugal injector placing safety door 11 and a PRP extraction injector taking safety door 12, the middle part of the aluminum profile combined frame 1 is fixedly provided with an equipment workbench 10, the aluminum profile combined frame 1 is divided into an upper layer and a lower layer by the equipment workbench 10, the centrifugal mechanism 2 is arranged at the lower layer, the centrifuge tube carrying mechanism 5 and the auxiliary liquid-transferring mechanism 7 are arranged at the upper layer, the centrifuge mechanism 2 is fixedly connected with the aluminum profile combined frame 1 through the centrifugal mechanism mounting plate 9, the auxiliary liquid-transferring mechanism 7 is fixedly connected with the aluminum profile combined frame 1 through the equipment workbench 10, centrifuge tube transport mechanism 5 is fixed to be set up in 1 upper strata rear side of aluminium alloy composite frame.
The centrifugal mechanism 2 consists of a variable frequency motor 13, a speed reducer 14, a synchronous belt and gear combined speed change mechanism mounting plate 15, a synchronous belt and gear combined speed change mechanism 16, a centrifugal mechanism rotating main shaft 17, a combined centrifugal injector rotating disc 18 and a combined centrifugal injector gravity overturning mechanism 19, wherein the variable frequency motor 13 is fixedly connected with the speed reducer 14, the speed reducer 14 is arranged on the right side of the upper surface of the centrifugal mechanism mounting plate 9, the synchronous belt and gear combined speed change mechanism mounting plate 15 fixedly connected with the centrifugal mechanism mounting plate 9 is arranged on the upper surface of the speed reducer 14, the synchronous belt and gear combined speed change mechanism 16 connected with an output shaft of the speed reducer 14 is fixedly arranged right above the synchronous belt and gear combined speed change mechanism mounting plate 15, the centrifugal mechanism rotating main shaft 17 is the tail end output shaft section of the synchronous belt and gear combined speed change mechanism 16, the centrifugal mechanism rotating main shaft 17 passes through the equipment workbench 10 to be connected with the combined centrifugal injector rotating disc 18, the combined centrifugal injector rotating disc 18 is V-shaped, the combined centrifugal injector gravity turnover mechanisms 19 are arranged on two sides of the combined centrifugal injector rotating disc 18, and the combined centrifugal injector 3 and the balance weight combined pipe 4 are respectively arranged in the two combined centrifugal injector gravity turnover mechanisms 19.
The auxiliary pipetting mechanism 7 is composed of a centrifuge tube auxiliary disassembly pipetting frame 6, a PRP extraction injector 8, an auxiliary pipetting mechanism mounting plate 36, a pipetting frame Z, a motor lead screw linear module 37, a combined centrifuge tube disassembly auxiliary clamping jaw 38, an auxiliary pipetting mechanism X, a motor lead screw linear module 39, a servo rotating disk 40, an injection end sealing clamping jaw 41, a pipette connecting head clamping jaw 42, a liquid separating pipette 43, a red blood cell plasma recovery bag 44, a PRP extraction injector fixing frame 45, a first liquid separating pipette sealing clamping jaw 46 and a second liquid separating pipette sealing clamping jaw 47, the auxiliary pipetting mechanism 7 is installed on the right side of the upper surface of the equipment workbench 10 through the auxiliary pipetting mechanism mounting plate 36, the centrifuge tube auxiliary disassembly pipetting frame 6 and the auxiliary pipetting mechanism X are installed on the auxiliary pipetting mechanism mounting plate 36, the auxiliary pipetting mechanism X is located in front of the centrifuge tube auxiliary disassembly pipetting frame 6, install on the supplementary liquid frame 37 that moves of dismantling of centrifuging tube and move liquid frame Z to motor lead screw straight line module 37, move liquid frame Z and install combination centrifuging tube and dismantle supplementary clamping jaw 38 on the slip table of motor lead screw straight line module 37, install two servo rotary disks 40 on the slip table of supplementary liquid moving mechanism X to motor lead screw straight line module 39, install injection end closing cap clamping jaw 41 and pipette connector clamping jaw 42 on two servo rotary disks 40 respectively, place in the clamping jaw gap of pipette connector clamping jaw 42 and divide liquid pipette 43.
The centrifuge tube carrying mechanism 5 comprises an X-direction motor lead screw linear transmission module 28, a centrifuge tube carrying and liquid transferring auxiliary gripper 29, a Z-direction motor lead screw linear transmission module 30, an electric rotating main shaft 31, an electric rotating main shaft mounting frame 32, a four-jaw chuck type pneumatic finger 33, a push rod gripping gripper mounting frame 34 and a push rod gripping gripper 35, wherein the X-direction motor lead screw linear transmission module 28 is arranged above the equipment workbench 10 and is mounted on two side columns of the aluminum profile combined frame 1, the centrifuge tube carrying and liquid transferring auxiliary gripper 29 is mounted on a sliding table of the X-direction motor lead screw linear transmission module 28, the centrifuge tube carrying and liquid transferring auxiliary gripper 29 is fixedly connected with the sliding table of the X-direction motor lead screw linear transmission module 28 through the electric rotating main shaft mounting frame 32, and a vertically downward electric rotating main shaft 31 is mounted above the electric rotating main shaft mounting frame 32, an output shaft of the electric rotating main shaft 31 is fixedly connected with a four-jaw chuck type pneumatic finger 33, a push rod grabbing clamping jaw mounting frame 34 is installed on one side of the electric rotating main shaft mounting frame 32, and a push rod grabbing clamping jaw 35 is installed at the tail end of the bottom of the push rod grabbing clamping jaw mounting frame 34.
The liquid separating pipette 43 is integrally manufactured, and comprises a red blood cell plasma recovery bag 44, a combined centrifugal injector connector 48, a PRP extraction injector connector 49, a first branch pipe 50 and a second branch pipe 51, wherein the combined centrifugal injector connector 48 is fixedly arranged at the connecting end of the liquid separating pipette 43 and the combined centrifugal injector 3, one end of the liquid separating pipette 43 with the combined centrifugal injector connector 48 is clamped in the positive center of a pipette connector clamping jaw 42, the branch pipes at the two ends of the liquid separating pipette 43 respectively penetrate through a first liquid separating pipette sealing clamping jaw 46 and a second liquid separating pipette sealing clamping jaw 47, the liquid separating pipette 43 and the PRP extraction injector 8 connecting end are fixedly provided with the PRP extraction injector connector 49, one end with the PRP extraction injector connector 49 penetrates through the second liquid separating pipette sealing clamping jaw 47 and the PRP extraction injector fixing frame 45 to be clamped and fixed, one end of the separating pipette 43 with the red blood cell plasma recovery bag 44 passes through the first separating pipette closing jaw 46, and the red blood cell plasma recovery bag 44 is placed in the bottom space of the first separating pipette closing jaw 46.
The combined centrifugal injector 3 and the counterweight combined tube 4 have the same structure and are composed of a combined centrifuge tube liquid storage tube 20, an injection end mounting turnbuckle 21, a push rod end mounting turnbuckle 22, an injector push rod 23, a push rod limiting tube sleeve 24, a tube sleeve turnbuckle connecting groove 25, an injection end sealing cover 26 and a sealing cover turnbuckle connecting groove 27, and the combined centrifugal injector 3 is divided into two working forms, namely an injector form and a centrifuge tube form; the shape of the injector is as follows: is a basic form of a combined centrifugal syringe 3 and is used for extracting plasma and consists of a combined centrifugal tube liquid storage tube 20, a syringe push rod 23 and a corresponding needle head; centrifuge tube shape of the combined centrifugal syringe 3: after the plasma is extracted, the needle head is taken down, the injection end sealing cover 26 is fixedly arranged on the combined centrifuge tube liquid storage tube 20 through the buckle notch structure of the injection end mounting turnbuckle 21 and the sealing cover turnbuckle connecting groove 27, and the push rod limiting tube sleeve 24 is fixedly arranged on the combined centrifuge tube liquid storage tube 20 through the buckle notch structure of the push rod end mounting turnbuckle 22 and the tube sleeve turnbuckle connecting groove 25 and is used for being placed in the centrifugal mechanism 2 for centrifugal operation.
As shown in fig. 1 to 9, the embodiment specifically is as follows: manually carrying out blood sampling operation through the syringe shape of the combined centrifugal syringe 3, after the blood sampling is finished, installing a push rod limiting pipe sleeve 24 and an injection end sealing cover 26 on a combined centrifuge tube liquid storage pipe 20 to enable the combined centrifugal syringe 3 to be in a centrifuge tube heart state, opening the combined centrifugal syringe placement safety door 11 at one side of the aluminum profile combined frame 1, vertically inserting the combined centrifugal syringe 3 downwards into a combined centrifugal syringe gravity turnover mechanism 19 of the centrifugal mechanism 2, placing the weighted balance combined pipe 4 into the combined centrifugal syringe gravity turnover mechanism 19 at the other side of the centrifugal mechanism 2, closing the combined centrifugal syringe placement safety door 11, opening the PRP extraction syringe extraction safety door 12 at one side of the aluminum profile combined frame 1, placing a liquid separating pipette 43 on the auxiliary liquid transfer mechanism 7 according to a corresponding state, closing the PRP extraction syringe extraction safety door 12 after placement, switching on a power supply of the workstation and starting the workstation; the combined centrifugal injector 3 firstly carries out centrifugal operation in the centrifugal mechanism 2, after the centrifugal operation is finished, the centrifuge tube carrying mechanism 5 grabs the combined centrifugal injector 3 through the four-jaw chuck type pneumatic finger 33, carries the combined centrifugal injector 3 to the position of the combined centrifuge tube dismounting auxiliary clamping jaw 38 of the auxiliary liquid-moving mechanism 7, after the combined centrifugal injector 3 is clamped by the combined centrifuge tube dismounting auxiliary clamping jaw 38, the electric rotating main shaft 31 on the centrifuge tube carrying mechanism 5 rotates to drive the four-jaw chuck type pneumatic finger 33 to take down the push rod limiting pipe sleeve 24 at the upper end of the combined centrifugal injector 3, meanwhile, the servo rotating disk 40 on the X-direction motor screw rod linear module 39 sliding table of the auxiliary liquid-moving mechanism moves to the position under the combined centrifugal injector 3 at the bottom of the combined centrifugal injector 3, and the injection end sealing cover clamping jaw 41 is in central symmetry with the combined centrifugal injector 3, the liquid moving frame Z moves downwards to the motor screw linear module 37, after the injection end sealing cover clamping jaw 41 clamps the injection end sealing cover 26, the servo rotating disc 40 below the injection end sealing cover clamping jaw 41 slightly rotates, simultaneously, the liquid moving frame Z slightly moves upwards to the motor screw linear module 37, the injection end sealing cover 26 at the bottom of the combined centrifugal injector 3 is taken down, then the auxiliary liquid moving mechanism X moves to the sliding table above the motor screw linear module 39, the servo rotating disc 40 at the other end is aligned with the injection port of the combined centrifugal injector 3, at the moment, the combined centrifugal injector connector 48 clamped at the center of the pipette connector clamping jaw 42 is positioned right below the combined centrifugal injector 3, the liquid moving frame Z moves downwards to the motor screw linear module 37, simultaneously, the servo rotating disc 40 slightly rotates, so that the combined centrifugal injector 3 is connected with the combined centrifugal injector connector 48, meanwhile, the first branch pipe 50 is loosened by the first liquid-dividing pipette closed clamping jaw 46, the second branch pipe 51 is clamped by the second liquid-dividing pipette closed clamping jaw 47, the first branch pipe 50 is ensured to be a passage, the second branch pipe 51 is a closed circuit, the injector push rod 23 is clamped by the push rod grabbing clamping jaw 35 on the centrifuge tube carrying and liquid-transferring auxiliary gripper 29, and the second liquid-dividing pipette closed clamping jaw 47 loosens the second branch pipe 51 by slowly moving downwards through the Z-direction motor screw rod linear transmission module 30, the erythrocyte plasma is pushed into the erythrocyte plasma recovery bag 44 through the first branch pipe 50, after the erythrocyte plasma is discharged, the first liquid-dividing pipette closed clamping jaw 46 clamps the first branch pipe 50, the second liquid-dividing pipette closed clamping jaw 47 loosens the second branch pipe 51, at the moment, the second branch pipe 51 is a passage, the first branch pipe 50 is a closed circuit, the Z-direction motor screw rod linear transmission module 30 continues to drive the push rod grabbing clamping jaw 35 to move downwards slowly, and push the PRP into the PRP extraction injector 8, and (3) closing the power supply of the workstation until the whole extraction process is finished, opening the PRP extraction injector to take out the safety door 12, and taking out the PRP extraction injector 8, wherein the PRP extraction injector 8 is the PRP to be extracted.
The process from centrifugation to PRP extraction is realized automatically, the operation is simple, and the labor is saved; in the extraction process, the plasma is not exposed to the air, so that the possibility of plasma pollution is reduced; the extracted PRP is directly placed in a syringe, so that the use or the storage is convenient; the discharged erythrocyte plasma is directly injected into the recovery bag, so that the subsequent treatment is convenient, the whole automation of the process from centrifugation to PRP extraction can be realized, the operation is simple and convenient, the labor is saved, the plasma pollution is reduced, and the efficiency of the enrichment method of the platelet-rich plasma is improved.
The points to be finally explained are: first, in the description of the present invention, it should be noted that, unless otherwise specified and limited, the terms "mounted," "connected," and "connected" should be understood broadly, and may be a mechanical connection or an electrical connection, or a communication between two elements, and may be a direct connection, "upper," "lower," "left," and "right," and the like are only used to indicate a relative positional relationship, and when the absolute position of the object to be described is changed, the relative positional relationship may be changed.
Secondly, the method comprises the following steps: in the drawings of the disclosed embodiments of the present invention, only the structures related to the disclosed embodiments are referred to, and other structures may refer to general designs, and the same embodiment and different embodiments of the present invention may be combined with each other without conflict.
And finally: the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention are intended to be included in the scope of the present invention.
Claims (8)
1. The utility model provides a platelet rich plasma automatic extraction device which characterized in that: including aluminium alloy combination frame (1), centrifugation mechanism (2), centrifuging tube transport mechanism (5) and supplementary move liquid mechanism (7), aluminium alloy combination frame (1) bottom mounting is equipped with centrifugation mechanism mounting panel (9), the lateral wall and the back wall punishment of aluminium alloy combination frame (1) do not fixedly are equipped with combination centrifugation syringe and place emergency exit (11) and PRP and draw syringe and take out emergency exit (12), aluminium alloy combination frame (1) middle part is fixed and is equipped with equipment workstation (10), aluminium alloy combination frame (1) is divided into upper and lower two-layer by equipment workstation (10), centrifugation mechanism (2) sets up in the lower floor, centrifuging tube transport mechanism (5) and supplementary move liquid mechanism (7) and set up in the upper strata, and centrifugation mechanism (2) is through centrifugation mechanism mounting panel (9) and aluminium alloy combination frame (1) fixed connection, the auxiliary liquid transfer mechanism (7) is fixedly connected with the aluminum profile combined frame (1) through an equipment workbench (10), and the centrifuge tube carrying mechanism (5) is fixedly arranged on the rear side of the upper layer of the aluminum profile combined frame (1);
Centrifugal mechanism (2) is by inverter motor (13), speed reducer (14), hold-in range and gear combination speed change mechanism mounting panel (15), hold-in range and gear combination speed change mechanism (16), centrifugal mechanism rotation main shaft (17), combination centrifugal syringe rolling disc (18) and combination centrifugal syringe gravity tilting mechanism (19) are constituteed, inverter motor (13) and speed reducer (14) fixed connection, and speed reducer (14) are installed on the upper surface of centrifugal mechanism mounting panel (9) right side partially, and the upper surface of speed reducer (14) is installed and is made up speed change mechanism mounting panel (15) with hold-in range and gear combination speed change mechanism of centrifugal mechanism mounting panel (9) fixed connection, and hold-in range and gear combination speed change mechanism (16) with speed reducer (14) output shaft hookup are fixed mounting directly over hold-in range and gear combination speed change mechanism mounting panel (15), and centrifugal mechanism rotates main shaft (17) and is hold-in range and gear combination speed change mechanism (16)'s (16) very end output A spindle section, wherein a rotating main shaft (17) of a centrifugal mechanism penetrates through an equipment workbench (10) to be connected with a rotating disc (18) of a combined centrifugal injector, the shape of the rotating disc (18) of the combined centrifugal injector is arranged in a V shape, combined centrifugal injector gravity turnover mechanisms (19) are arranged on two sides of the rotating disc (18) of the combined centrifugal injector, and a combined centrifugal injector (3) and a counterweight combined pipe (4) are respectively arranged in the two combined centrifugal injector gravity turnover mechanisms (19);
Supplementary pipetting mechanism (7) are by the supplementary dismantlement of centrifuging tube liquid transfer frame (6), PRP draws syringe (8), supplementary pipetting mechanism mounting panel (36), pipetting frame Z is to motor lead screw straight line module (37), combination centrifuging tube dismantles supplementary clamping jaw (38), supplementary pipetting mechanism X is to motor lead screw straight line module (39), servo rotary disk (40), injection end closing cap clamping jaw (41), pipette connector clamping jaw (42), divide liquid pipette (43), red cell plasma recovery bag (44), PRP draws syringe mount (45), first minute liquid pipette closing jaw (46) and second minute liquid pipette closing jaw (47) are constituteed, supplementary pipetting mechanism (7) are installed in the right side of equipment workstation (10) upper surface through supplementary pipetting mechanism mounting panel (36), install the supplementary dismantlement of centrifuging tube liquid transfer frame (6) and supplementary pipetting mechanism X on supplementary pipetting mechanism mounting panel (36) and to motor lead screw straight line module (39) ) The utility model discloses a liquid transfer machine, supplementary liquid-transfering mechanism X is located the dead ahead of centrifuging tube auxiliary disassembly liquid-transfering frame (6) to motor lead screw straight line module (39), install liquid-transfering frame Z to motor lead screw straight line module (37) on centrifuging tube auxiliary disassembly liquid-transfering frame (6), liquid-transfering frame Z installs combination centrifuging tube dismantlement auxiliary clamping jaw (38) to the slip table of motor lead screw straight line module (37), install two servo rotary disk (40) on the slip table of supplementary liquid-transfering mechanism X to motor lead screw straight line module (39), install injection end closing cap clamping jaw (41) and pipette connector clamping jaw (42) on two servo rotary disk (40) respectively, liquid (43) have been placed in the clamping jaw gap of pipette connector clamping jaw (42).
2. The automatic platelet-rich plasma extraction device according to claim 1, wherein: centrifuging tube transport mechanism (5) include that X snatchs clamping jaw mounting bracket (34) and push rod snatchs clamping jaw (35) to motor lead screw straight line transmission module (28), centrifuging tube transport and move liquid supplementary tongs (29), Z to motor lead screw straight line transmission module (30), electric rotating main shaft (31), electric rotating main shaft mounting bracket (32), four-jaw chuck formula pneumatic finger (33), push rod.
3. The automatic platelet-rich plasma extraction device according to claim 2, wherein: the X-direction motor screw rod linear transmission module (28) is arranged above the equipment workbench (10) and is arranged on the upright posts at the two sides of the aluminum profile combined frame (1), a centrifuge tube carrying and liquid transferring auxiliary gripper (29) is arranged on a sliding table of the X-direction motor lead screw linear transmission module (28), the centrifuge tube carrying and liquid transferring auxiliary gripper (29) is fixedly connected with the sliding table of the X-direction motor lead screw linear transmission module (28) through an electric rotating main shaft mounting frame (32), a vertically downward electric rotating main shaft (31) is arranged above the electric rotating main shaft mounting rack (32), an output shaft of the electric rotating main shaft (31) is fixedly connected with a four-jaw chuck type pneumatic finger (33), a push rod grabbing clamping jaw mounting frame (34) is installed on one side of the electric rotating main shaft mounting frame (32), and a push rod grabbing clamping jaw (35) is installed at the tail end of the bottom of the push rod grabbing clamping jaw mounting frame (34).
4. The automatic platelet-rich plasma extraction device according to claim 1, wherein: the liquid separating pipette (43) is integrally manufactured and comprises a red blood cell plasma recovery bag (44), a combined centrifugal injector connector (48), a PRP extraction injector connector (49), a first branch pipe (50) and a second branch pipe (51).
5. The automatic platelet-rich plasma extraction device according to claim 4, wherein: the liquid separating pipette (43) is fixedly provided with a combined centrifugal injector connector (48) with the connecting end of the combined centrifugal injector (3), one end of the liquid separating pipette (43) with the combined centrifugal injector connector (48) is clamped in the positive center of a pipette connector clamping jaw (42), branch tubes at two ends of the liquid separating pipette (43) respectively pass through a first liquid separating pipette closed clamping jaw (46) and a second liquid separating pipette closed clamping jaw (47), the liquid separating pipette (43) and a PRP extraction injector (8) connecting end are fixedly provided with a PRP extraction injector connector (49), one end with the PRP extraction injector connector (49) passes through a second liquid separating pipette closed clamping jaw (47) and the PRP extraction injector fixing frame (45) and is clamped and fixed, and one end of the liquid separating pipette (43) with a red cell plasma recovery bag (44) passes through the first liquid separating pipette closed clamping jaw (46), the red blood cell plasma recovery bag (44) is placed in the bottom void of the first pipette tip (46).
6. The automatic platelet-rich plasma extraction device according to claim 1, wherein: the combined centrifugal injector (3) is identical in structure with the counterweight combined tube (4), and consists of a combined centrifugal tube liquid storage tube (20), an injection end mounting turnbuckle (21), a push rod end mounting turnbuckle (22), an injector push rod (23), a push rod limiting tube sleeve (24), a tube sleeve turnbuckle connecting groove (25), an injection end sealing cover (26) and a sealing cover turnbuckle connecting groove (27), and the combined centrifugal injector (3) is divided into two working forms, namely an injector form and a centrifugal tube form.
7. The automatic platelet-rich plasma extraction device according to claim 6, wherein: the shape of the injector is as follows: consists of a combined centrifuge tube liquid storage tube (20), a syringe push rod (23) and a corresponding needle head.
8. The automatic platelet-rich plasma extraction device according to claim 6, wherein: the centrifuge tube shape: the buckle notch structure of the injection end mounting turnbuckle (21) and the sealing cover turnbuckle connecting groove (27) enables the injection end sealing cover (26) to be fixedly installed on the combined centrifuge tube liquid storage tube (20), and the buckle notch structure of the push rod end mounting turnbuckle (22) and the tube sleeve turnbuckle connecting groove (25) enables the push rod limiting tube sleeve (24) to be fixedly installed on the combined centrifuge tube liquid storage tube (20).
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| CN202110955130.0A CN113499609A (en) | 2021-08-19 | 2021-08-19 | Platelet-rich plasma automatic extraction device |
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| CN114209911A (en) * | 2021-12-21 | 2022-03-22 | 鲲石生物科技(深圳)有限公司 | Blood cell separation system |
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