CN107198893A - Leucocyte-removing PRP preparation method - Google Patents

Leucocyte-removing PRP preparation method Download PDF

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Publication number
CN107198893A
CN107198893A CN201710529142.0A CN201710529142A CN107198893A CN 107198893 A CN107198893 A CN 107198893A CN 201710529142 A CN201710529142 A CN 201710529142A CN 107198893 A CN107198893 A CN 107198893A
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China
Prior art keywords
prp
layers
leucocyte
syringe
blood
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CN201710529142.0A
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Chinese (zh)
Inventor
巩家富
巩杰
张加琪
王进
田茂清
李磊磊
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Priority to CN201710529142.0A priority Critical patent/CN107198893A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02—Separation of non-miscible liquids
    • B01D17/0217—Separation of non-miscible liquids by centrifugal force
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/16—Blood plasma; Blood serum

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Thermal Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • External Artificial Organs (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to a kind of leucocyte-removing PRP preparation method, it is characterized in that, comprise the following steps：(1) anti-coagulants is added in syringe, blood is extracted；(2) whole bloods in 500g 800g, 4 DEG C of centrifugation 10min, test tube are carried out first and are divided into 3 layers, upper strata is serum, and lower floor is red blood cell, visible one layer of two layers of intersection very thin pale yellow chromatograph, i.e. PRP layers；(3) serum and PRP layers and PRP layers lower 1 3mm red blood cells are moved on in another centrifuge tube with syringe, mixed, static 20 40min at 4 DEG C uses filter for removing white blood cell filtrating leukocytes；(4) suspension is being subjected to 500g 800g, 4 DEG C of centrifugation 10min, supernatant removed, remaining is PRP；(5) PRP is resuspended with a small amount of supernatant, it is standby.This method can effectively remove leucocyte, to meet use demand.

Description

Leucocyte-removing PRP preparation method
Technical field：
The present invention relates to a kind of leucocyte-removing PRP preparation method.
Background technology：
The PRP used on the market at present prepares suit, and the PRP (platelet rich plasma) of preparation is general containing substantial amounts of white Cell, when for wound, the effect with part anti-inflammation and sterilization, but situations such as such as pulled muscle, pulled tendon When, the presence of leucocyte can cause local inflammation reaction, influence therapeutic effect, or even can suppress the recovery of damage location.
The content of the invention：
The present invention will provide a kind of preparation method for the leucocyte-removing PRP that can effectively remove leucocyte.
Leucocyte-removing PRP provided by the present invention preparation method, it is characterized in that, comprise the following steps：
(1) anti-coagulants is added in syringe, blood is extracted；
(2) whole blood carried out first in 500g-800g, 4 DEG C of centrifugation 10min, test tube is divided into 3 layers, and upper strata is serum, lower floor It is red blood cell, visible one layer of two layers of intersection very thin pale yellow chromatograph, i.e. PRP layers；
(3) serum and PRP layers and PRP layers lower 1-3mm red blood cells are moved on in another centrifuge tube with syringe, mixed, The static 20-40min at 4 DEG C, uses filter for removing white blood cell filtrating leukocytes；
(4) suspension is being subjected to 500g-800g, 4 DEG C of centrifugation 10min, supernatant removed, remaining is PRP；
(5) PRP is resuspended with a small amount of supernatant, it is standby.
Red blood cell layer is removed in described step (2).
Extracted in described step (1) after blood, the blood of extraction turns upside down mixing in syringe, is put into 3-5 ° of ice In case, 40min is freezed.
The beneficial effects of the invention are as follows：
Leucocyte can be effectively removed, to meet use demand.
Specific embodiment：
Leucocyte-removing PRP provided by the present invention preparation method, wherein specific embodiment 1, comprise the following steps：
(1) anti-coagulants is added in syringe, blood is extracted；
(2) whole blood carried out first in 500g-800g, 4 DEG C of centrifugation 10min, test tube is divided into 3 layers, and upper strata is serum, lower floor It is red blood cell, visible one layer of two layers of intersection very thin pale yellow chromatograph, i.e. PRP layers；
(3) serum and PRP layers and PRP layers lower 1-3mm red blood cells are moved on in another centrifuge tube with syringe, mixed, The static 20-40min at 4 DEG C, uses filter for removing white blood cell filtrating leukocytes；
(4) suspension is being subjected to 500g-800g, 4 DEG C of centrifugation 10min, supernatant removed, remaining is PRP；
(5) PRP is resuspended with a small amount of supernatant, it is standby.
Embodiment 2：Red blood cell layer is removed in described step (2).
Embodiment 3：Extracted in described step (1) after blood, the blood of extraction turns upside down mixing in syringe, puts Enter in 3-5 ° of refrigerator, freeze 40min.
Above-mentioned case study on implementation is only for clear example of the present invention, and to be not the limit to embodiment of the present invention It is fixed.The obvious changes or variations amplified out the spirit for belonging to the present invention is still in protection scope of the present invention.

Claims (3)

1. a kind of leucocyte-removing PRP preparation method, it is characterized in that, comprise the following steps：
(1) anti-coagulants is added in syringe, blood is extracted；
(2) whole blood carried out first in 500g-800g, 4 DEG C of centrifugation 10min, test tube is divided into 3 layers, and upper strata is serum, and lower floor is red Cell, visible one layer of two layers of intersection very thin pale yellow chromatograph, i.e. PRP layers；
(3) serum and PRP layers and PRP layers lower 1-3mm red blood cells are moved on in another centrifuge tube with syringe, mixed, at 4 DEG C Under static 20-40min, use filter for removing white blood cell filtrating leukocytes；
(4) suspension is being subjected to 500g-800g, 4 DEG C of centrifugation 10min, supernatant removed, remaining is PRP；
(5) PRP is resuspended with a small amount of supernatant, it is standby.
2. leucocyte-removing PRP according to claim 1 preparation method, it is characterized in that, removed in described step (2) red Cellular layer.
3. leucocyte-removing PRP according to claim 1 preparation method, it is characterized in that, blood is extracted in described step (1) After liquid, the blood of extraction turns upside down mixing in syringe, is put into 3-5 ° of refrigerator, freezes 40min.
CN201710529142.0A 2017-07-01 2017-07-01 Leucocyte-removing PRP preparation method Pending CN107198893A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710529142.0A CN107198893A (en) 2017-07-01 2017-07-01 Leucocyte-removing PRP preparation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710529142.0A CN107198893A (en) 2017-07-01 2017-07-01 Leucocyte-removing PRP preparation method

Publications (1)

Publication Number Publication Date
CN107198893A true CN107198893A (en) 2017-09-26

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CN201710529142.0A Pending CN107198893A (en) 2017-07-01 2017-07-01 Leucocyte-removing PRP preparation method

Country Status (1)

Country Link
CN (1) CN107198893A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109692761A (en) * 2018-12-25 2019-04-30 山东博森医学工程技术有限公司 A kind of platelet rich plasma extraction element and its method
WO2021006839A1 (en) * 2019-07-09 2021-01-14 Gazi̇ Endüstri̇ Anoni̇m Şi̇rketi̇ Method for obtaining solid plasma as a filling material
US11944667B2 (en) * 2018-12-07 2024-04-02 Biotherapy Services Limited Methods of making a wound treatment composition

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1276725A (en) * 1997-10-17 2000-12-13 丰收技术股份有限公司 Precipitation of growth-factor-enriched fibrinogen concentrate from platelet rich plasma
EP1723959A1 (en) * 2004-01-30 2006-11-22 Emi Sumida Composition comprising platelet-rich plasma
CN101528243A (en) * 2006-10-27 2009-09-09 尼普洛株式会社 Platelet-rich plasma separator and platelet-rich plasma separation method
CN102421362A (en) * 2009-05-14 2012-04-18 生物技术研究所I+D有限公司 Method for preparing at least one compound from blood, and sampling device for use when carrying out said method
CN104337835A (en) * 2014-11-13 2015-02-11 河南金泰生物技术股份有限公司 Preparation method for platelet rich plasma (PRP)
CN104548232A (en) * 2013-10-15 2015-04-29 金鸿 Device and method of extracting high-concentration plasma from whole blood
WO2016109655A1 (en) * 2014-12-31 2016-07-07 Anthrogenesis Corporation Methods for isolation of platelets

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1276725A (en) * 1997-10-17 2000-12-13 丰收技术股份有限公司 Precipitation of growth-factor-enriched fibrinogen concentrate from platelet rich plasma
EP1723959A1 (en) * 2004-01-30 2006-11-22 Emi Sumida Composition comprising platelet-rich plasma
CN101528243A (en) * 2006-10-27 2009-09-09 尼普洛株式会社 Platelet-rich plasma separator and platelet-rich plasma separation method
CN102421362A (en) * 2009-05-14 2012-04-18 生物技术研究所I+D有限公司 Method for preparing at least one compound from blood, and sampling device for use when carrying out said method
CN104548232A (en) * 2013-10-15 2015-04-29 金鸿 Device and method of extracting high-concentration plasma from whole blood
CN104337835A (en) * 2014-11-13 2015-02-11 河南金泰生物技术股份有限公司 Preparation method for platelet rich plasma (PRP)
WO2016109655A1 (en) * 2014-12-31 2016-07-07 Anthrogenesis Corporation Methods for isolation of platelets

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11944667B2 (en) * 2018-12-07 2024-04-02 Biotherapy Services Limited Methods of making a wound treatment composition
CN109692761A (en) * 2018-12-25 2019-04-30 山东博森医学工程技术有限公司 A kind of platelet rich plasma extraction element and its method
WO2021006839A1 (en) * 2019-07-09 2021-01-14 Gazi̇ Endüstri̇ Anoni̇m Şi̇rketi̇ Method for obtaining solid plasma as a filling material

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Application publication date: 20170926

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