CN109666591B - 提高紫球藻中藻红素含量的方法 - Google Patents
提高紫球藻中藻红素含量的方法 Download PDFInfo
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- CN109666591B CN109666591B CN201910109463.4A CN201910109463A CN109666591B CN 109666591 B CN109666591 B CN 109666591B CN 201910109463 A CN201910109463 A CN 201910109463A CN 109666591 B CN109666591 B CN 109666591B
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- porphyridium
- phycoerythrin
- content
- inoculating
- nitrogen
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- 108010004729 Phycoerythrin Proteins 0.000 title claims abstract description 49
- 241000206618 Porphyridium Species 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000005286 illumination Methods 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 64
- 229910052757 nitrogen Inorganic materials 0.000 claims description 32
- 238000012258 culturing Methods 0.000 claims description 17
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 16
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims description 15
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 claims description 15
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 12
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 235000010344 sodium nitrate Nutrition 0.000 claims description 8
- 239000004317 sodium nitrate Substances 0.000 claims description 7
- 239000003617 indole-3-acetic acid Substances 0.000 claims description 6
- 239000004115 Sodium Silicate Substances 0.000 claims description 5
- FRHBOQMZUOWXQL-UHFFFAOYSA-L ammonium ferric citrate Chemical compound [NH4+].[Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FRHBOQMZUOWXQL-UHFFFAOYSA-L 0.000 claims description 5
- 230000003698 anagen phase Effects 0.000 claims description 5
- 229910021538 borax Inorganic materials 0.000 claims description 5
- 229960004642 ferric ammonium citrate Drugs 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000004313 iron ammonium citrate Substances 0.000 claims description 5
- 235000000011 iron ammonium citrate Nutrition 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- 238000010899 nucleation Methods 0.000 claims description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 5
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 5
- 239000004328 sodium tetraborate Substances 0.000 claims description 5
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 5
- 241000195493 Cryptophyta Species 0.000 abstract description 20
- 230000012010 growth Effects 0.000 abstract description 8
- 239000000243 solution Substances 0.000 description 21
- 210000004027 cell Anatomy 0.000 description 16
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- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- 241000234427 Asparagus Species 0.000 description 4
- 235000005340 Asparagus officinalis Nutrition 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000003375 plant hormone Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 241001428407 Ceramium Species 0.000 description 2
- 241000206572 Rhodophyta Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 125000001477 organic nitrogen group Chemical group 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000001054 red pigment Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 241000199913 Crypthecodinium Species 0.000 description 1
- 108010053210 Phycocyanin Proteins 0.000 description 1
- 241000206609 Porphyra Species 0.000 description 1
- 241000206607 Porphyra umbilicalis Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
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- 230000003203 everyday effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000000168 pyrrolyl group Chemical group 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
Abstract
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CN201910109463.4A CN109666591B (zh) | 2019-02-11 | 2019-02-11 | 提高紫球藻中藻红素含量的方法 |
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CN201910109463.4A CN109666591B (zh) | 2019-02-11 | 2019-02-11 | 提高紫球藻中藻红素含量的方法 |
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CN109666591A CN109666591A (zh) | 2019-04-23 |
CN109666591B true CN109666591B (zh) | 2022-07-12 |
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CN111172096B (zh) * | 2020-04-11 | 2022-04-12 | 杭州渔森农业技术开发有限公司 | 一种高密度培养异胶藻的生产工艺 |
CN114854597B (zh) * | 2022-07-05 | 2022-09-23 | 烟台泓源生物肥料有限公司 | 一种雨生红球藻培养基 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101463320A (zh) * | 2007-12-17 | 2009-06-24 | 华北制药集团有限责任公司 | 一种生物氮素及其制备方法和应用 |
CN101941869A (zh) * | 2010-09-01 | 2011-01-12 | 山东圣鹏农药有限公司 | 一种含芸苔素内酯和磷酸二氢钾的植物生长促进剂组合物及应用 |
CN106942226A (zh) * | 2017-03-27 | 2017-07-14 | 武汉中博水产生物技术有限公司 | 促进虾、蟹塘中水草和有益藻类生长的植物生长调节剂 |
CN108410737A (zh) * | 2018-05-28 | 2018-08-17 | 中国科学院南海海洋研究所 | 一种紫球藻的两步培养法 |
CN109258448A (zh) * | 2018-09-12 | 2019-01-25 | 集美大学 | 一种紫菜藻红蛋白高效富集的培养方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2009335976A1 (en) * | 2008-12-19 | 2011-08-11 | Alpha-J Research Limited Partnership | Optimization of algal product production through uncoupling cell proliferation and algal product production |
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2019
- 2019-02-11 CN CN201910109463.4A patent/CN109666591B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101463320A (zh) * | 2007-12-17 | 2009-06-24 | 华北制药集团有限责任公司 | 一种生物氮素及其制备方法和应用 |
CN101941869A (zh) * | 2010-09-01 | 2011-01-12 | 山东圣鹏农药有限公司 | 一种含芸苔素内酯和磷酸二氢钾的植物生长促进剂组合物及应用 |
CN106942226A (zh) * | 2017-03-27 | 2017-07-14 | 武汉中博水产生物技术有限公司 | 促进虾、蟹塘中水草和有益藻类生长的植物生长调节剂 |
CN108410737A (zh) * | 2018-05-28 | 2018-08-17 | 中国科学院南海海洋研究所 | 一种紫球藻的两步培养法 |
CN109258448A (zh) * | 2018-09-12 | 2019-01-25 | 集美大学 | 一种紫菜藻红蛋白高效富集的培养方法 |
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Effective date of registration: 20231116 Address after: No. 305, Liming, Yushan Village, Yushan Township, Fuyang District, Hangzhou City, Zhejiang Province, 310000 Patentee after: HANGZHOU YUSEN AGRICULTURAL TECHNOLOGY DEVELOPMENT Co.,Ltd. Address before: 311400 building 6, No.3 Shangzhuang Road, Changkou Industrial Park, Fuyang District, Hangzhou City, Zhejiang Province Patentee before: HANGZHOU YUANTAI BIOTECHNOLOGY Co.,Ltd. |
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Denomination of invention: A method to increase the content of phycoerythrin in purple algae Effective date of registration: 20231219 Granted publication date: 20220712 Pledgee: Zhejiang Fuyang Rural Commercial Bank Co.,Ltd. Lishan Sub branch Pledgor: HANGZHOU YUSEN AGRICULTURAL TECHNOLOGY DEVELOPMENT Co.,Ltd. Registration number: Y2023980072199 |