CN109663661B - Separation method for shelled artemia cysts after hatching - Google Patents
Separation method for shelled artemia cysts after hatching Download PDFInfo
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- CN109663661B CN109663661B CN201811474410.4A CN201811474410A CN109663661B CN 109663661 B CN109663661 B CN 109663661B CN 201811474410 A CN201811474410 A CN 201811474410A CN 109663661 B CN109663661 B CN 109663661B
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- separation
- nauplii
- hatching
- artemia
- fresh water
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- 238000000926 separation method Methods 0.000 title claims abstract description 76
- 241001247197 Cephalocarida Species 0.000 title claims abstract description 34
- 208000031513 cyst Diseases 0.000 title claims abstract description 25
- 230000012447 hatching Effects 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 235000013601 eggs Nutrition 0.000 claims abstract description 22
- -1 peroxide compound Chemical class 0.000 claims abstract description 19
- 239000013505 freshwater Substances 0.000 claims abstract description 15
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 239000012267 brine Substances 0.000 claims abstract description 7
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims abstract description 7
- 150000003839 salts Chemical class 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims description 18
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 206010011732 Cyst Diseases 0.000 claims description 2
- 241000133262 Nauplius Species 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 125000000864 peroxy group Chemical group O(O*)* 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 239000012535 impurity Substances 0.000 abstract description 3
- 239000000126 substance Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000238557 Decapoda Species 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 210000003278 egg shell Anatomy 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 208000003243 intestinal obstruction Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
- B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
- B03B5/28—Washing granular, powdered or lumpy materials; Wet separating by sink-float separation
- B03B5/30—Washing granular, powdered or lumpy materials; Wet separating by sink-float separation using heavy liquids or suspensions
- B03B5/44—Application of particular media therefor
- B03B5/442—Application of particular media therefor composition of heavy media
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a separation liquid after hatching of shelled artemia cysts and a separation method thereof, wherein the separation liquid comprises a first separation liquid and a second separation liquid; the first separation liquid comprises fresh water and peroxide compound, and the ratio of the peroxide compound to the fresh water is 0.2-1: 2.5-5; the second separation liquid comprises high salinity brine with the salt concentration of 100-. The separation liquid and the separation method thereof after the shelling artemia cysts are incubated solve the problem of separation of hollow membranes, dead eggs and impurities after the artemia cysts are shelled and incubated, and ensure safe and convenient use of artemia nauplii.
Description
Technical Field
The invention belongs to the field of artemia nauplii application, and particularly relates to a separation solution for hatching unshelled artemia eggs and a separation method thereof.
Background
The artemia cysts are high-quality bait for the fry of the aquatic products and have important function in the aquaculture industry. The artemia cysts contain ferrous lipoprotein, and the fry and shrimp cannot be digested, so that the artemia larva generally needs to be hatched first, and then the larva is used as bait to feed. The egg shells and the larvae after hatching are not easy to be completely separated, and the loss of part of the hatched larvae along with the falling of the shells is easy to cause; or the egg shell and the larva are taken together to cause the intestinal obstruction of the fish and shrimp seedlings to die.
The shelled artemia cysts are a novel product, and the artemia cysts can be directly fed after shelling and can also be hatched into larvae. Because no egg shell exists, the large energy consumption of the larva during shell breaking can be reduced, and more nutrition can be kept. In the process of raising fish and shrimp seedlings, the cost of artemia cysts usually accounts for 50-70% of the cost of a seedling raising field. Through the mode of shelling, can improve the hatchability and make the artemia larva keep more nutrition, can also utilize the low-grade ovum that a large amount of hatchability is low simultaneously, reduce the cost of growing seedlings, it is significant to the seedling field.
However, after the eggs of the artemia cysts with shells are hatched into nauplii, the hatching mixture comprises unhatched dead eggs, empty shells and nauplii; the mixture of the shelled artemia cysts after hatching is dead eggs, empty membranes and nauplii; because the mixture is different, the separation method and the separation process are different. Therefore, the prior art methods for separation of hatching mixtures of artemia cysts cannot be followed for the separation of nauplii.
The development of a separation process suitable for use after hatching of shelled artemia cysts is urgently needed.
Disclosure of Invention
In view of the above, the invention aims to provide a separation solution for shelled artemia cysts after hatching and a separation method thereof, so that the problems of membrane empty, dead eggs and impurity separation after shelling and hatching of the artemia cysts are solved, and the safe and convenient use of the artemia nauplii is ensured.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
a separation liquid after hatching of shelled artemia cysts comprises a first separation liquid and a second separation liquid; the first separation liquid comprises fresh water and peroxide compound, and the volume ratio of the peroxide compound to the fresh water is 0.2-1: 2.5-5; the second separation liquid comprises high salinity brine with the salt concentration of 100-.
Preferably, in the first separation liquid, the volume ratio of the peroxide compound to the fresh water is 1: 5.
preferably, the peroxy compound comprises a peroxy-containing compound; preferred peroxides include hydrogen peroxide or carbon peroxide.
Preferably, the hydrogen peroxide is a liquid and has a concentration of 3 to 30%.
Preferably, the salt concentration of the high salinity brine is 200-.
A separation method after hatching of shelled artemia cysts adopts the separation liquid to separate a shelled artemia cyst hatching mixture, and comprises the following steps:
step 1: adjusting the density of nauplii in the unshelled artemia egg hatching mixture to 7000-10000/mL, putting the nauplii into a separation barrel, adding first separation liquid into the separation barrel, firstly adding fresh water, uniformly mixing, then adding peroxide compound, and mixing according to the ratio of nauplii (g) to fresh water volume (mL) and peroxide compound (mL) of 1-5: 2.5-5: 0.2-1 addition;
step 2: standing for 5-10 min, and collecting the first isolate at the bottom;
and step 3: adjusting the density of the nauplii in the first separation to 7000-: 5-6 adding;
and 4, step 4: stirring, standing for 5-15min, and collecting the second upper separated matter as separated nauplius larva.
Preferably, in step 2, 80% of the empty egg membranes and 10% of the dead eggs are removed from the first isolate.
Preferably, in step 4, the separation efficiency of the nauplii of the second isolate is 99%.
Compared with the prior art, the separation solution and the separation method thereof after hatching the shelled artemia cysts have the following advantages:
(1) according to the characteristics of the mixture after hatching the unshelled artemia cysts, and according to the properties of different specific gravities, oxidation dissolution speeds and the like of empty membranes, dead eggs and impurities, twice separation is designed, so that the separation effect is good, and the separation efficiency of the nauplii is high.
(2) The first separation liquid and the second separation liquid are designed to adapt to the technological process of two times of separation, so that the method is more pertinent, and saves resources and time.
(3) The first separation liquid adopts fresh water and hydrogen peroxide, most of dead eggs settle due to the large specific gravity, the empty membrane is supported under the action of the hydrogen peroxide, and the first-time separator at the bottom is collected, so that the separation efficiency of removing 80% of the empty egg membrane and 10% of the dead eggs is realized;
(4) in the second separation process, dead eggs and nauplii are screened by using the salinity of the strong brine, the salinity is 100-.
Drawings
FIG. 1 is a photograph of an nauplii microscopically examined second isolate as a real object in example 2 of the present invention.
Detailed Description
Unless defined otherwise, technical terms used in the following examples have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.
The present invention will be described in detail with reference to examples.
Example one
Putting the mixture of the hatched artemia cysts into a separation barrel, filtering redundant water by using filter cloth to ensure that the density of nauplii is 7000/mL-10000/mL, taking 400g of the mixture, adding 500mL of fresh water, then adding 100mL of 3 wt% hydrogen peroxide, discharging from the bottom of the separation barrel after reacting for 5 minutes, and collecting a first-time separator, wherein 80% of empty egg membranes and 10% of dead eggs are removed; and pouring the collected first-time separated substance into a separation barrel again, wherein the density of the nauplii is 7000/mL-10000/mL, the weight is 400g, 500mL of high-salinity water with the salinity of 200 per mill is added, the mixture is uniformly stirred and then stands for 20 minutes, the lower part of the mixture is discharged, and the second-time separated substance on the upper part of the mixture is collected to obtain the separated nauplii.
The nauplii are in perfect shapes and have no damage through microscopic examination, and the nauplii separation efficiency reaches 99 percent.
Example two
Putting the unshelled artemia egg hatching mixture into a separation barrel, filtering redundant water by using filter cloth to ensure that the density of nauplii is 7000/mL-10000/mL, taking 300Kg, adding 500L of fresh water, then adding 20L of 30 wt% hydrogen peroxide, discharging the nauplii from the bottom of the separation barrel after reacting for 10 minutes, and collecting a first-time separator, wherein 80% of empty egg membranes and 10% of dead eggs are removed; and pouring the collected first-time separated substance into a separation barrel again, wherein the density of the nauplii is 7000/mL-10000/mL, the weight is 300Kg, 600L of high-salinity water with the salinity of 300 per mill is added, the mixture is uniformly stirred and then stands for 20 minutes, the lower part of the mixture is discharged, and the second-time separated substance on the upper part of the mixture is collected to obtain the separated nauplii.
The nauplii are in perfect shapes and have no damage through microscopic examination, and the nauplii separation efficiency reaches 99 percent.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. A separation method of shelled artemia cysts after hatching is characterized in that: separating the shelled artemia cyst hatching mixture by using a separation solution comprising a first separation solution and a second separation solution; the first separation liquid comprises fresh water and peroxide compound, and the volume ratio of the peroxide compound to the fresh water is 0.2-1: 2.5-5; the second separation liquid comprises high salinity brine, and the salt concentration of the high salinity brine is 100-;
the separation comprises the following steps:
step 1: adjusting the density of nauplii in the unshelled artemia egg hatching mixture to 7000-10000/mL, putting the nauplii into a separation barrel, adding first separation liquid into the separation barrel, firstly adding fresh water, uniformly mixing, then adding peroxide compound, and mixing according to the ratio of nauplii (g) to fresh water volume (mL) and peroxide compound (mL) of 1-5: 2.5-5: 0.2-1 addition;
step 2: standing for 5-10 min, and collecting the first isolate at the bottom;
and step 3: adjusting the density of the nauplii in the first separation to 7000-10000/mL, pouring the nauplii into a separation barrel, adding a second separation liquid inwards, and adjusting the volume (mL) of the nauplii (g) and the second separation liquid to 3-4: 5-6 adding;
and 4, step 4: stirring, standing for 5-15min, and collecting the second upper separated matter as separated nauplius larva.
2. The separation method according to claim 1, characterized in that: in the first separated liquid, the volume ratio of the peroxide compound to the fresh water is 1: 5.
3. the separation method according to claim 1, characterized in that: the peroxy compound comprises a peroxy group-containing compound.
4. The separation method according to claim 3, characterized in that: the peroxy compound includes hydrogen peroxide or carbon peroxide.
5. The separation method according to claim 4, characterized in that: the hydrogen peroxide is liquid and has a concentration of 3-30%.
6. The separation method according to claim 1, characterized in that: the salt concentration of the high salinity brine is 200-.
7. The separation method according to claim 1, characterized in that: in step 2, 80% of the empty egg membranes and 10% of the dead eggs were removed from the first isolate.
8. The method for post-hatching separation of shelled artemia cysts according to claim 1, wherein: in step 4, the separation efficiency of the nauplii of the second isolate reaches 99%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811474410.4A CN109663661B (en) | 2018-12-04 | 2018-12-04 | Separation method for shelled artemia cysts after hatching |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811474410.4A CN109663661B (en) | 2018-12-04 | 2018-12-04 | Separation method for shelled artemia cysts after hatching |
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| CN109663661A CN109663661A (en) | 2019-04-23 |
| CN109663661B true CN109663661B (en) | 2021-02-23 |
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| CN201811474410.4A Active CN109663661B (en) | 2018-12-04 | 2018-12-04 | Separation method for shelled artemia cysts after hatching |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110622895A (en) * | 2019-10-18 | 2019-12-31 | 塔里木大学 | Schizothorax prenanti artificial propagation spawning device |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1063801A (en) * | 1991-02-09 | 1992-08-26 | 辽宁师范大学科技开发部 | Artemia cysts processing technology and equipment thereof shell |
| CN1076827A (en) * | 1992-04-01 | 1993-10-06 | 李文 | The new method that artemia larvae separates |
| EP1836901A1 (en) * | 2006-03-23 | 2007-09-26 | INVE Technologies NV | Method for producing a feed comprising Artemia nauplii |
| CN103350026A (en) * | 2013-07-19 | 2013-10-16 | 中盐制盐工程技术研究院 | Method for separating artemia nauplii and unhatched artemia cysts |
| DE102015015609B3 (en) * | 2015-12-03 | 2016-08-18 | Lars Remke | Method for separating hatched Artemia from their dura eggs and separator for Artemia permanent eggs |
| CN206686954U (en) * | 2017-04-18 | 2017-12-01 | 天津渤海水产研究所 | The portable separator of indoor artemia hatching |
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