CN109645026B - Composite disinfectant for silkworm - Google Patents
Composite disinfectant for silkworm Download PDFInfo
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- CN109645026B CN109645026B CN201811607284.5A CN201811607284A CN109645026B CN 109645026 B CN109645026 B CN 109645026B CN 201811607284 A CN201811607284 A CN 201811607284A CN 109645026 B CN109645026 B CN 109645026B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/04—Sulfonic acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- Pest Control & Pesticides (AREA)
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Abstract
The invention provides a compound disinfectant for silkworms, and particularly relates to the technical field of prevention and treatment of silkworm diseases. The composite disinfectant consists of main agent, assistant and desiccant, the main agent is prepared by mixing 120 weight portions of chlorine dioxide powder and 80-100 weight portions of basic magnesium hypochlorite, the assistant is 40-60 weight portions of dodecyl ethoxy sulfobetaine as zwitterionic surfactant, and the desiccant is 80-100 weight portions of silica gel. The composite disinfectant for silkworms has the characteristics of good stability, wide bactericidal spectrum, good bactericidal effect, high efficiency, no influence of environmental conditions and environmental protection.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of prevention and treatment of silkworm diseases, and particularly relates to a compound disinfectant for silkworms.
[ background of the invention ]
In the process of silkworm breeding, disinfection is the premise of preventing and treating silkworm diseases and ensuring high yield and high quality of silkworm breeding, so that the disinfection work is particularly important. There are five main classes of disinfectants currently on the market: the disinfectant containing chlorine has wide application and good effect, but is easily influenced by the concentration of effective chlorine, the pH value, the time, the temperature, the concentration of organic matters in water and the like; the aldehyde disinfectant has strong killing capability on pathogenic microorganisms, is not influenced at all, but has strong volatility and irritation on human bodies, skin, eyes and mucous membranes; the surfactant disinfectant has narrow sterilization spectrum and high sterilization concentration, and needs to be matched with other disinfectants to improve the sterilization effect; the sulfur-containing disinfectant is only effective on fungi and ticks, has little effect on other pathogens, and is effective only when requiring a closed environment and higher humidity and temperature; the strong basicity of lime can make the virus polyhedron shell quickly dissolve, so that the virus ions are exposed and easy to kill, but after the lime absorbs the moisture of air, the disinfection effect is lost.
In the prior art, in the research on the disinfectant for silkworms, a liquid disinfectant is manufactured in a patent document with a Chinese patent publication number of CN104825945A named as a disinfectant for silkworms, and has the advantages of wide sterilization spectrum, good sterilization effect, short period, no chemical residue and no pollution to the environment, but the humidity of a silkworm room is increased in the disinfection process, so that the growth of the silkworms is not facilitated. The invention discloses a gas disinfectant for silkworms and a patent document applying the same, wherein the Chinese patent publication No. CN105053001A is named as a gas disinfectant for silkworms, and the gas disinfectant for silkworms is prepared by reacting raw materials consisting of chlorine dioxide liquid medicine and citric acid liquid according to the volume ratio of 1:1 in a reactor to generate gas for disinfection.
Therefore, on the premise of ensuring the disinfection effect, the invention provides a nontoxic, efficient, environment-friendly and convenient disinfectant for silkworm production, and has important significance.
[ summary of the invention ]
The invention aims to: the composite disinfectant for the silkworms has the characteristics of good stability, wide bactericidal spectrum, good bactericidal effect, high efficiency, no influence of environmental conditions and environmental protection.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a composite disinfectant for silkworm is prepared from chlorine dioxide powder and basic magnesium hypochlorite through mixing, surfactant chosen from laurylethoxy sulfobetaine and silica gel as assistant, and drying agent chosen from silica gel.
The use method of the compound disinfectant comprises the following steps: before disinfection, the silkworm chamber and the silkworm tool are cleaned by clean water, air-dried for 12 hours, then the main agent is uniformly spread in the silkworm chamber and on the silkworm tool, after the main agent stays for 1-2 hours, the main agent powder is cleaned and recovered, then the auxiliary agent is prepared into 5% -10% aqueous solution and sprayed on the silkworm chamber and the silkworm tool, after 20-30 minutes, the auxiliary agent is cleaned by clean water, and finally, drying agents are placed on the silkworm chamber and the silkworm seat to enable the silkworm chamber to be in a dry state.
Optimally, the particle size of the main agent powder is 300-400 meshes.
Preferably, the auxiliary agent is added into clear water before use to prepare 5-10% aqueous solution.
Further preferably, the primary agent, the secondary agent and the drying agent are packaged separately and independently.
Further optimized, the compound disinfectant for silkworms is used in silkworm rearing rooms and silkworm rearing instruments, but not used in silkworm bodies and mulberry leaves.
Still further preferably, the pH of the sterilization environment is between 7.5 and 8.5.
And further optimizing, wherein the relative humidity after drying is 45-55%.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the disinfectant main agent is formed by combining chlorine dioxide and basic magnesium hypochlorite, the chlorine dioxide is a broad-spectrum and efficient disinfectant, the disinfectant has high stability, is non-toxic and is slightly influenced by temperature and pH value, the effective chlorine is as high as 260 percent, and all microorganisms including bacterial spores can be killed; basic magnesium hypochlorite is an inorganic antibacterial agent, is insoluble in water, is non-toxic, is quite stable in air, has about 40 percent of available chlorine, and has excellent disinfection and sterilization capabilities. The effect of chlorine-containing disinfectants is determined by oxidation, chlorine acts on certain enzymes to interfere cell metabolism and cause death of pathogenic microorganisms, and available chlorine refers to the amount of chlorine with oxidation capacity, so that the higher the available chlorine, the better the oxidation capacity and the better the disinfection effect. The synergistic effect of chlorine dioxide and basic magnesium hypochlorite enhances the oxidation effect of available chlorine, and further increases the disinfection effect without being influenced by temperature, pH value, time and the like.
2. The whole disinfection environment is under the condition of pH value of 7.5-8.5, the alkalescence is favorable for silkworm breeding, and can inhibit inactivation of NPV and CPV which are two main pathogens of the silkworm industry, thereby improving the disinfection effect.
3. The disinfectant auxiliary agent adopts a zwitterionic surfactant dodecyl ethoxy sulfobetaine, the zwitterionic surfactant has the detergency of an anionic surfactant and the bactericidal effect of a cationic surfactant, has no stimulation to skin and low corrosivity, is used after being matched with a chlorine-containing disinfectant, can remove microbial bacterial debris on the surface of an object, achieves the effect of secondary disinfection, prevents bacterial regeneration, and has biodegradability and no pollution to the environment.
4. After disinfection, a drying agent is added because the silkworm needs to be in a dry breeding environment, silica gel is used as the drying agent of the silkworm rearing instrument in the silkworm rearing room, and the silica gel is a high-microporous-structure water-containing materialSilicon dioxideIs nontoxic, tasteless and non-toxicSmelling, stable chemical property and strong moisture absorption performance, and is a high-activity adsorption material. The dry environment is beneficial to the breeding of the silkworm, and can also inhibit the growth of bacteria, fungi and the like, thereby prolonging the disinfection effect.
[ detailed description ] embodiments
The present invention will be further described with reference to the following examples.
Example 1
A composite disinfectant for silkworm is composed of main agent, auxiliary agent and drying agent, which are packed separately. The main agent is formed by mixing 100 parts of chlorine dioxide powder and 80 parts of basic magnesium hypochlorite by weight, and the granularity is 300-400 meshes; the auxiliary agent is 40 parts of a zwitterionic surfactant dodecyl ethoxy sulfobetaine, and before the auxiliary agent is used, the auxiliary agent is added into clear water to prepare a 5% -10% aqueous solution; the drying agent is 80 parts of silica gel.
The use method of the compound disinfectant comprises the following steps: before disinfection, the silkworm chamber and the silkworm tool are cleaned by clean water, air-dried for 12 hours, then the main agent is uniformly scattered in the silkworm chamber and on the silkworm tool, after the main agent stays for 1-2 hours, the main agent powder is cleaned and recovered, then the auxiliary agent is prepared into 5% -10% aqueous solution and sprayed on the silkworm chamber and the silkworm tool, after 20-30 minutes, the auxiliary agent is cleaned by clean water, and finally, drying agents are placed on the silkworm chamber and the silkworm seat to enable the silkworm chamber to be in a dry state, and the relative humidity after drying is 45% -55%. The pH value of the disinfection environment is between 8 and 9.
The compound disinfectant for silkworm is used in silkworm room and silkworm tool, but not in silkworm body and mulberry leaf.
Example 2
A composite disinfectant for silkworm is composed of main agent, auxiliary agent and drying agent, which are packed separately. The main agent is formed by mixing 120 parts of chlorine dioxide powder and 100 parts of basic magnesium hypochlorite by weight, and the granularity is 300-400 meshes; 60 parts of zwitterionic surfactant dodecyl ethoxy sulfobetaine serving as an auxiliary agent is added into clear water before use to prepare a 5-10% aqueous solution; the drying agent is 100 parts of silica gel.
The use method of the compound disinfectant comprises the following steps: before disinfection, the silkworm chamber and the silkworm tool are cleaned by clean water, air-dried for 12 hours, then the main agent is uniformly scattered in the silkworm chamber and on the silkworm tool, after the main agent stays for 1-2 hours, the main agent powder is cleaned and recovered, then the auxiliary agent is prepared into 5% -10% aqueous solution and sprayed on the silkworm chamber and the silkworm tool, after 20-30 minutes, the auxiliary agent is cleaned by clean water, and finally, drying agents are placed on the silkworm chamber and the silkworm seat to enable the silkworm chamber to be in a dry state, and the relative humidity after drying is 45% -55%. The pH value of the disinfection environment is between 8 and 9.
The compound disinfectant for silkworm is used in silkworm room and silkworm tool, but not in silkworm body and mulberry leaf.
Example 3
A composite disinfectant for silkworm is composed of main agent, auxiliary agent and drying agent, which are packed separately. The main agent is formed by mixing 105 parts of chlorine dioxide powder and 85 parts of basic magnesium hypochlorite by weight, and the granularity is 300-400 meshes; the auxiliary agent is 45 parts of a zwitterionic surfactant dodecyl ethoxy sulfobetaine, and before the auxiliary agent is used, the auxiliary agent is added into clear water to prepare a 5% -10% aqueous solution; the drying agent is 85 parts of silica gel.
The use method of the compound disinfectant comprises the following steps: before disinfection, the silkworm chamber and the silkworm tool are cleaned by clean water, air-dried for 12 hours, then the main agent is uniformly scattered in the silkworm chamber and on the silkworm tool, after the main agent stays for 1-2 hours, the main agent powder is cleaned and recovered, then the auxiliary agent is prepared into 5% -10% aqueous solution and sprayed on the silkworm chamber and the silkworm tool, after 20-30 minutes, the auxiliary agent is cleaned by clean water, and finally, drying agents are placed on the silkworm chamber and the silkworm seat to enable the silkworm chamber to be in a dry state, and the relative humidity after drying is 45% -55%. The pH value of the disinfection environment is between 8 and 9.
The compound disinfectant for silkworm is used in silkworm room and silkworm tool, but not in silkworm body and mulberry leaf.
Example 4
A composite disinfectant for silkworm is composed of main agent, auxiliary agent and drying agent, which are packed separately. The main agent is formed by mixing 115 parts of chlorine dioxide powder and 95 parts of basic magnesium hypochlorite by weight, and the granularity is 300-400 meshes; 55 parts of a zwitterionic surfactant dodecyl ethoxy sulfobetaine as an auxiliary agent, and the auxiliary agent is added into clear water to prepare a 5-10% aqueous solution before use; the drying agent is 95 parts of silica gel.
The use method of the compound disinfectant comprises the following steps: before disinfection, the silkworm chamber and the silkworm tool are cleaned by clean water, air-dried for 12 hours, then the main agent is uniformly scattered in the silkworm chamber and on the silkworm tool, after the main agent stays for 1-2 hours, the main agent powder is cleaned and recovered, then the auxiliary agent is prepared into 5% -10% aqueous solution and sprayed on the silkworm chamber and the silkworm tool, after 20-30 minutes, the auxiliary agent is cleaned by clean water, and finally, drying agents are placed on the silkworm chamber and the silkworm seat to enable the silkworm chamber to be in a dry state, and the relative humidity after drying is 45% -55%. The pH value of the disinfection environment is between 8 and 9.
The compound disinfectant for silkworm is used in silkworm room and silkworm tool, but not in silkworm body and mulberry leaf.
Example 5
A composite disinfectant for silkworm is composed of main agent, auxiliary agent and drying agent, which are packed separately. The main agent is formed by mixing 110 parts of chlorine dioxide powder and 90 parts of basic magnesium hypochlorite by weight, and the granularity is 300-400 meshes; 50 parts of a zwitterionic surfactant dodecyl ethoxy sulfobetaine serving as an auxiliary agent is added into clear water before use to prepare a 5-10% aqueous solution; the drying agent is 90 parts of silica gel.
The use method of the compound disinfectant comprises the following steps: before disinfection, the silkworm chamber and the silkworm tool are cleaned by clean water, air-dried for 12 hours, then the main agent is uniformly scattered in the silkworm chamber and on the silkworm tool, after the main agent stays for 1-2 hours, the main agent powder is cleaned and recovered, then the auxiliary agent is prepared into 5% -10% aqueous solution and sprayed on the silkworm chamber and the silkworm tool, after 20-30 minutes, the auxiliary agent is cleaned by clean water, and finally, drying agents are placed on the silkworm chamber and the silkworm seat to enable the silkworm chamber to be in a dry state, and the relative humidity after drying is 45% -55%. The pH value of the disinfection environment is between 8 and 9.
The compound disinfectant for silkworm is used in silkworm room and silkworm tool, but not in silkworm body and mulberry leaf.
Example 6
The disinfectant was obtained according to the example described in a patent document entitled disinfectant for silkworms having chinese patent publication No. CN 104825945A.
Example 7
Fresh lime powder was used as the disinfectant.
Example 8
The disinfectant was obtained in the same manner as in example 5, except that the adjuvant zwitterionic surfactant lauryl ethoxy sulfobetaine was not added.
Example 9
Comparison of Experimental results
1. Sample preparation: disinfectants were prepared as in examples 1-8, with samples 1-8 numbered separately.
2. Test methods and evaluation methods: at the same time and place, samples 1-8 were used to sterilize the rearing room rearing bed, and after sterilization, the sterilization rate, the microbial survival rate after 30 hours, the young silkworm qualification rate and the silkworm pupa qualification rate were measured. The total number of pathogenic bacteria in a rearing room and rearing bed is measured before disinfection, the number of the residual pathogenic bacteria is measured after disinfection, the number of the pathogenic bacteria is measured once again after 30 hours, and the number of 1-year silkworms, the number of 4-year silkworms and the number of 5-year silkworms are measured.
Sterilizing rate, determining the number of residual microorganisms/determining the total number of microorganisms in rearing room before sterilizing
Determining the total number of microorganisms in the rearing room and rearing bed before disinfection after 30 hr
The qualification rate of young silkworms is 1 instar/4 instar
The qualification rate of silkworm chrysalis is 1 age silkworm/5 age silkworm
3. The experimental results are as follows: according to the measured data, the experimental results of table 1 are obtained;
TABLE 1 results of the experiment
4. The experimental results are as follows:
as can be seen from the experimental results in Table 1, samples 1 to 5 are superior to samples 6 to 8 in terms of sterilization rate, microbial survival rate after 30 hours, and young silkworm qualification rate.
In the patent document named as a disinfectant for silkworms, which is published under the Chinese patent No. CN104825945A, sample 6 obtained by the above example had a good sterilization rate, but had a poor persistence, which affected the qualification rate of young silkworms.
Sample 7 used fresh lime powder as a disinfectant, and the sterilization rate, the microbial survival rate after 30 hours and the pass rate of young silkworms were all inferior to those of samples 1 to 5.
The adjuvant zwitterionic surfactant dodecyl ethoxy sulfobetaine is not added to the sample 8, the disinfectant is obtained in the same way as the disinfectant obtained in the example 5, the adjuvant is not added, the disinfection is not thorough, and the qualification rate of young silkworms and the qualification rate of silkworm chrysalis are reduced.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (8)
1. The composite disinfectant for silkworm is characterized by comprising a main agent, an auxiliary agent and a drying agent, wherein the main agent is formed by mixing 120 parts by weight of chlorine dioxide powder and 80-100 parts by weight of basic magnesium hypochlorite, the auxiliary agent is 40-60 parts by weight of a zwitterionic surfactant dodecyl ethoxy sulfobetaine, and the drying agent is 80-100 parts by weight of silica gel; the use method of the compound disinfectant comprises the following steps: cleaning a silkworm rearing room and a silkworm rearing instrument with clean water before disinfection, drying for 12 hours in the air, uniformly scattering a main agent in the silkworm rearing room and on the silkworm rearing instrument, cleaning and recovering main agent powder after the main agent stays for 1-2 hours, spraying an auxiliary agent prepared into an aqueous solution on the silkworm rearing room and the silkworm rearing instrument, cleaning the auxiliary agent with clean water after 20-30 minutes, and placing drying agents on the silkworm rearing room and a silkworm rearing bed when silkworms reach 4 years old so as to keep a silkworm rearing environment in a dry state.
2. The compound disinfectant for silkworms as claimed in claim 1, wherein the compound disinfectant comprises a main agent, an auxiliary agent and a drying agent, wherein the main agent is prepared by mixing 115 parts by weight of chlorine dioxide powder 105-115 parts and 85-95 parts by weight of basic magnesium hypochlorite, the auxiliary agent is 45-55 parts by weight of zwitterionic surfactant dodecyl ethoxy sulfobetaine, and the drying agent is 85-95 parts by weight of silica gel.
3. The complex disinfectant for silkworms of claim 1 or 2, wherein the main agent, the auxiliary agent and the drying agent are separately packaged.
4. The complex disinfectant for silkworm as claimed in claim 1 or 2, wherein the particle size of the main agent is 300-400 mesh.
5. The disinfectant composition according to claim 1 or 2, wherein the adjuvant is added to clean water to prepare a 5% -10% aqueous solution before use.
6. The disinfectant composition according to claim 1 or 2, wherein the disinfectant composition is used in silkworm rearing rooms and rearing instruments, and is not used in silkworm rearing bodies and mulberry leaves.
7. The complex disinfectant for silkworms of claim 1, wherein the relative humidity after drying is 45% to 55%.
8. The complex disinfectant for silkworms of claim 1 or 2, wherein the pH of the disinfection environment is between 7.5 and 8.5.
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CN114586783B (en) * | 2022-03-23 | 2023-10-20 | 湖南普泰尔环境股份有限公司 | Efficient deodorizing disinfectant and preparation method and application thereof |
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