CN109644948A - A kind of experimental provision and application for laboratory cultures earthworm - Google Patents

A kind of experimental provision and application for laboratory cultures earthworm Download PDF

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Publication number
CN109644948A
CN109644948A CN201910003065.4A CN201910003065A CN109644948A CN 109644948 A CN109644948 A CN 109644948A CN 201910003065 A CN201910003065 A CN 201910003065A CN 109644948 A CN109644948 A CN 109644948A
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China
Prior art keywords
incubator
interior
earthworm
experimental provision
laboratory cultures
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Withdrawn
Application number
CN201910003065.4A
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Chinese (zh)
Inventor
董炜华
刘宝林
宋扬
李晓强
李明
杨翔宇
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Changchun Normal University
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Changchun Normal University
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Priority to CN201910003065.4A priority Critical patent/CN109644948A/en
Publication of CN109644948A publication Critical patent/CN109644948A/en
Withdrawn legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0332Earthworms

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Processing Of Solid Wastes (AREA)
  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of experimental provision for laboratory cultures earthworm and applications, it accurately the various parameters to earthworm can carry out real-time monitoring and control, to guarantee earthworm well-grown, different batches consistency is good, it can ensure the repeatability and science tested, and, the present invention is provided with miniature ventilation assembly in the bottom of interior incubator, it may be implemented to carry out ventilation appropriate in bottom, improve the oxygen content of the decomposed compost of biology, guarantee the growth conditions of earthworm, the present invention is simple and convenient to the collection of earthworm, the design that interior incubator can skid off, it can be in order to clearing up it, and, temperature control is carried out by external incubator, the temperature of interior incubator can be made more preferably stable, realize accurately controlling for parameter.

Description

A kind of experimental provision and application for laboratory cultures earthworm
Technical field
The present invention relates to a kind of experimental provision for laboratory cultures earthworm and applications, belong to the experiment and cultivation of earthworm Equipment technical field.
Background technique
Earthworm is both consumer, analyst and attemperator in the ecosystem, is changed to soil fertility and the soil texture Change plays a significant role.It by feeding, digestion, the activity such as drain and burrow there is weight to soil nutrient and soil physico-chemical property Want meaning.Meanwhile the correlation as animals and plants and microorganism in important ecological factor and the ecosystem is also very It is worth fastidious.Earthworm is located at the bottom of land ecologic food chain, has significant enrichment to a variety of environmental contaminants, point Cloth is extensive, is the good instruction biology for monitoring soil and water body environment pollution situation.
For the culture experiment of earthworm, the cultivation parameter of earthworm is different, and the earthworm growth conditions of each batch have Biggish difference, this is for the culture research of earthworm, it is difficult to ensure the repeatability and science of experiment.But city at present Special equipment is cultivated without experiment earthworm in face, usually carries out in the incubator or directly buys ready-made earthworm, this training method The earthworm turned out is difficult to realize effective intelligent control, moreover, current incubator is excessively closed, it is easy to earthworm be caused to lack The problems such as oxygen, and heating temperature is also unstable, and this generates biggish adverse effect for the growth of earthworm compared with.
The present invention improves earthworm in view of the above problems, provide a kind of experimental provision for laboratory cultures earthworm and application The science and repeatability of earthworm culture experiment.
Summary of the invention
To achieve the above object, the invention provides the following technical scheme: a kind of experiment for laboratory cultures earthworm fills It sets comprising pedestal, sparge pipe, interior incubator, outer incubator, the decomposed compost of biology, interior ventilation assembly, is driven Liquid spraying pump Component and controller, wherein at least one outer incubator, the setting that can be skidded off in the outer incubator are provided on the pedestal Have interior incubator, the decomposed compost of biology be equipped in the interior incubator, which is characterized in that the bottom of the interior incubator with Cavity is provided between the inner bottom wall of the outer incubator, the bottom of the interior incubator is provided with interior ventilation assembly, described outer The temperature heating element that is controlled by the controller and can be guaranteed constant temperature is provided in incubator, the interior incubator One end is removably provided with the collecting box being collected to earthworm, and described to drive component setting separate in the interior incubator The side of the collecting box is provided with culture liquid case, the training to drive earthworm to the collecting box on the pedestal Nutrient solution case is connected to the sparge pipe by the Liquid spraying pump, and the sparge pipe protrudes into the interior incubator, and is located at described The top of the decomposed compost of biology, the interior incubator is interior to be sensed at least provided with temperature sensor, humidity sensor and pH value Device, the temperature sensor, humidity sensor and pH value sensor are controlled with the controller and are connected, to be passed according to temperature Culture solution concentration, culture solution in sensor, the value of feedback of humidity sensor and pH value sensor and set value calculation culture liquid case Spray volume and temperature heating element heating movement, be provided at least one separate ventilation hole on the outer incubator.
Further, preferably, one end of the close collecting box of the outer incubator is provided with chamber door, the bottom of the chamber door Portion is hingedly arranged on the cabinet of the outer incubator, the inside of the chamber door and outer incubator it is opposite with the chamber door Outer temperature sensor is provided on inside, the top of the interior incubator is provided with interior case using what fastening shoulder detachably fastened Lid.
Further, it preferably, the component of driving includes above driving lamp and lamp is driven in side, is set on the inside of the interior case lid It is equipped with reflective mirror, the center of the reflective mirror or the side far from collecting box are provided with and drive lamp, and lamp position is driven in the side In on the inside of the one side separate and opposite with the collecting box of the interior incubator.
Further, preferably, the interior incubator and interior case lid are all made of Heat Conduction Material and are made, and the interior culture Intensive on case to be provided with pore, the diameter of the pore is 0.1-0.2mm.
Further, preferably, the interior ventilation assembly includes mini fan, porous plate, ventilating board and bottom ventilation hole, In, the bottom of the interior incubator is provided with multiple bottom ventilation holes, and the bottom inside of the interior incubator is set using membrane support It is equipped with ventilating board, is provided with mini fan between the ventilating board and the bottom interior wall of the interior incubator, on the ventilating board The position of the corresponding mini fan is provided with inner vent, and the upper end support of the ventilating board is provided with porous plate, and biology is rotten Ripe compost is laid on the porous plate.
Further, preferably, the both ends of the inwardly angled incubator of the inner vent on the ventilating board are arranged, and institute The upper surface for stating ventilating board is provided with ventilation groove, and the ventilation groove is connected to each inner vent.
Further, preferably, the collecting box includes collection tank, hook, hanging seat, baffle, climbs out of hole, wherein institute The one end for stating interior incubator is provided with hanging seat, and the upper end of the collection tank is provided with hook, and the collection tank uses institute It states hook to be hung on the hanging seat, open at one end, the interior incubator close to the interior incubator of the collection tank It is upper to be located at the collection tank and the interior incubator contradicts to be provided at connection fragment position and multiple climbs out of hole, the interior incubator Inner wall on climb out of that the position in hole can plug together described in face be provided with baffle.
Further, preferably, the component of driving further includes electromagnetic viscosimeter component, the electromagnetic viscosimeter component is located at institute It states between ventilating board and the inner bottom wall of the interior incubator.
Further, preferably, the bottom of the interior incubator is arranged, there are two sliding blocks, and the bottom of the outer incubator is set It is equipped with sliding slot, the setting that the sliding block can slide into and out on the sliding slot, the decomposed compost of biology includes at least bottom sea Continuous layer, top spongy layer and biological feed layer, wherein the bottom spongy layer, top spongy layer are located at the biological feed layer The thickness of bottom and top, the top spongy layer is less than the bottom spongy layer.
Further, the present invention provides a kind of applications of experimental provision for laboratory cultures earthworm, it is characterised in that: It is applied to laboratory cultures earthworm, and carries out real-time monitoring and control to the culture parameters of earthworm, wherein culture parameters are at least Including temperature sensor, humidity sensor and pH value sensor.
Compared with prior art, the beneficial effects of the present invention are:
Experimental provision for laboratory cultures earthworm of the invention can accurately the various parameters to earthworm carry out in fact When monitoring and control, to guarantee earthworm well-grown, different batches consistency is good, it can be ensured that the repeatability and science of experiment Property, moreover, the present invention is provided with miniature ventilation assembly in the bottom of interior incubator, may be implemented to carry out in bottom appropriate logical Wind improves the oxygen content of the decomposed compost of biology, guarantees the growth conditions of earthworm, and the present invention is simple and convenient to the collection of earthworm, The design that interior incubator can skid off, can in order to clear up it, moreover, by external incubator carry out temperature control, can So that the temperature of interior incubator is more preferably stable, accurately controlling for parameter is realized.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the experimental provision entirety for laboratory cultures earthworm of the present invention;
Fig. 2 is a kind of structural representation of the decomposed compost of biology of experimental provision for laboratory cultures earthworm of the present invention Figure;
Fig. 3 is a kind of structural schematic diagram of the collecting box of the experimental provision for laboratory cultures earthworm of the present invention;
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment shall fall within the protection scope of the present invention.
Fig. 1-3 is please referred to, the present invention provides a kind of technical solution: a kind of experimental provision for laboratory cultures earthworm, It includes pedestal 4, Liquid spraying pump 1, sparge pipe 8, interior incubator 6, outer incubator 5, the decomposed compost 9 of biology, interior ventilation assembly, drive Catch up with component and controller, wherein be provided at least one outer incubator 5 on the pedestal 4, can skid off in the outer incubator 5 Be provided with interior incubator 6, the decomposed compost 9 of biology is equipped in the interior incubator 6, which is characterized in that the interior culture Cavity is provided between the bottom of case 6 and the inner bottom wall of the outer incubator, the bottom of the interior incubator is provided with interior ventilation Component is provided with the temperature heating element that is controlled by the controller and can be guaranteed constant temperature, institute in the outer incubator The one end for stating interior incubator 6 is removably provided with the collecting box 18 being collected to earthworm, and the component of driving is arranged in institute The side in interior incubator far from the collecting box is stated to be arranged on the pedestal to drive earthworm to the collecting box There is culture liquid case 2, the culture liquid case 2 is connected to the sparge pipe 8, the sparge pipe by pipe fitting 3 by the Liquid spraying pump 1 It protrudes into the interior incubator, and is located at the top of the decomposed compost of biology, at least provided with temperature in the interior incubator Spend sensor, humidity sensor and pH value sensor, the temperature sensor, humidity sensor and pH value sensor with it is described Controller control connection, so as to according to temperature sensor, the value of feedback of humidity sensor and pH value sensor and setting value control The heating movement of culture solution concentration, the spray volume of culture solution and temperature heating element, the outer incubator in system culture liquid case On be provided at least one separate ventilation hole.
In the present embodiment, one end of the close collecting box of the outer incubator is provided with chamber door 19, the chamber door 19 Bottom is hingedly arranged on the cabinet of the outer incubator, the inside of the chamber door and outer incubator it is opposite with the chamber door Inside on be provided with outer temperature sensor, the top of the interior incubator using fasten that shoulder detachably fastens be provided in Case lid 23.
The component of driving includes above driving lamp 7 and lamp 20 is driven in side, and the inside of the interior case lid 23 is provided with reflective mirror 24, the center of the reflective mirror 24 or the side far from collecting box are provided with and drive lamp 7, and the side drives lamp 20 and is located at institute It states on the inside of the one side separate and opposite with the collecting box of interior incubator.
As preferred embodiment, the interior incubator and interior case lid are all made of Heat Conduction Material and are made, and the interior training Support it is intensive on case be provided with pore, the diameter of the pore is 0.1-0.2mm.
As preferred embodiment, the interior ventilation assembly includes that mini fan 10, porous plate 26, ventilating board 16 and bottom are logical Air holes 22, wherein the bottom of the interior incubator is provided with multiple bottom ventilation holes 22, and the bottom inside of the interior incubator uses Membrane support is provided with ventilating board 16, is provided with mini fan between the ventilating board 16 and the bottom interior wall of the interior incubator 10, the position that the mini fan is corresponded on the ventilating board 16 is provided with inner vent, and the upper end support of the ventilating board is set It is equipped with porous plate 26, the decomposed compost of biology is laid on the porous plate 26.The inclination of inner vent on the ventilating board The both ends setting of inside incubator, and the upper surface of the ventilating board is provided with ventilation groove 27, the ventilation groove with it is each Inner vent connection.
As more preferably embodiment, the collecting box includes collection tank 18, hook 13, hanging seat 14, baffle 12, climbs out of hole 11, wherein the one end of the interior incubator is provided with hanging seat 14, and the upper end of the collection tank is provided with hook 13, described Collection tank is hung on the hanging seat 14 using described, and one end close to the interior incubator of the collection tank is opened Mouthful, it is located at the collection tank and the interior incubator conflict connection fragment position on the interior incubator and is provided with multiple climb out of Hole 11, climb out of that the position in hole can plug together on the inner wall of the interior incubator described in face is provided with baffle 12.
As more preferably embodiment, the component of driving further includes electromagnetic viscosimeter component 15, electromagnetic viscosimeter component position Between the ventilating board and the inner bottom wall of the interior incubator.
As more preferably embodiment, there are two sliding block 21, the bottoms of the outer incubator for the bottom setting of the interior incubator Portion is provided with sliding slot 17, the setting that the sliding block can slide into and out on the sliding slot, and the decomposed compost of biology at least wraps Include bottom spongy layer, top spongy layer 29 and biological feed layer 28, wherein the bottom spongy layer, top spongy layer 29 are located at described The thickness of the bottom and top of biological feed layer 28, the top spongy layer is less than the bottom spongy layer.
The present invention provides a kind of applications of experimental provision for laboratory cultures earthworm, it is characterised in that: it is applied Real-time monitoring and control are carried out in laboratory cultures earthworm, and to the culture parameters of earthworm, wherein culture parameters include at least temperature Spend sensor, humidity sensor and pH value sensor.
Experimental provision for laboratory cultures earthworm of the invention can accurately the various parameters to earthworm carry out in fact When monitoring and control, to guarantee earthworm well-grown, different batches consistency is good, it can be ensured that the repeatability and science of experiment Property, moreover, the present invention is provided with miniature ventilation assembly in the bottom of interior incubator, may be implemented to carry out in bottom appropriate logical Wind improves the oxygen content of the decomposed compost of biology, guarantees the growth conditions of earthworm, and the present invention is simple and convenient to the collection of earthworm, The design that interior incubator can skid off, can in order to clear up it, moreover, by external incubator carry out temperature control, can So that the temperature of interior incubator is more preferably stable, accurately controlling for parameter is realized.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (10)

1. a kind of experimental provision for laboratory cultures earthworm comprising pedestal, Liquid spraying pump, sparge pipe, interior incubator, outer training It supports case, the decomposed compost of biology, interior ventilation assembly, drive component and controller, wherein be provided at least one on the pedestal Outer incubator, what can be skidded off in the outer incubator is provided with interior incubator, and the decomposed training of biology is equipped in the interior incubator Support soil, which is characterized in that cavity is provided between the bottom of the interior incubator and the inner bottom wall of the outer incubator, it is described interior The bottom of incubator is provided with interior ventilation assembly, is provided in the outer incubator and is controlled and can be protected by the controller The temperature heating element of constant temperature is demonstrate,proved, one end of the interior incubator is removably provided with the collecting box being collected to earthworm, The side driven component and the separate collecting box in the interior incubator is set, to drive earthworm to the collection In case, culture liquid case is provided on the pedestal, the culture liquid case is connected to the sparge pipe by the Liquid spraying pump, described Sparge pipe protrudes into the interior incubator, and is located at the top of the decomposed compost of biology, at least sets in the interior incubator It is equipped with temperature sensor, humidity sensor and pH value sensor, the temperature sensor, humidity sensor and pH value sensor are equal It controls and connects with the controller, according to the value of feedback of temperature sensor, humidity sensor and pH value sensor and to set The heating movement of culture solution concentration, the spray volume of culture solution and temperature heating element, described outer in setting control culture liquid case At least one separate ventilation hole is provided on incubator.
2. a kind of experimental provision for laboratory cultures earthworm according to claim 1, it is characterised in that: the outer training The one end for supporting the close collecting box of case is provided with chamber door, and the cabinet of the outer incubator is hingedly arranged in the bottom of the chamber door On, outer temperature sensor, institute are provided on the inside opposite with the chamber door of the inside of the chamber door and outer incubator The top for stating interior incubator is provided with interior case lid using what fastening shoulder detachably fastened.
3. a kind of experimental provision for laboratory cultures earthworm according to claim 2, it is characterised in that: described to drive Component includes above driving lamp and lamp is driven in side, is provided with reflective mirror on the inside of the interior case lid, the center of the reflective mirror or Side far from collecting box, which is provided with, drives lamp, and lamp position is driven in the separate with the collecting box of the interior incubator in the side And on the inside of opposite one side.
4. a kind of experimental provision for laboratory cultures earthworm according to claim 3, it is characterised in that: the interior training It supports case and interior case lid to be all made of Heat Conduction Material and be made, and intensive on the interior incubator is provided with pore, the capillary The diameter in hole is 0.1-0.2mm.
5. a kind of experimental provision for laboratory cultures earthworm according to claim 1, it is characterised in that: described interior logical Wind component includes mini fan, porous plate, ventilating board and bottom ventilation hole, wherein the bottom of the interior incubator is provided with multiple The bottom inside of bottom ventilation hole, the interior incubator is provided with ventilating board, the ventilating board and the interior training using membrane support It supports and is provided with mini fan between the bottom interior wall of case, the position that the mini fan is corresponded on the ventilating board is provided with interior lead to Air holes, the upper end support of the ventilating board are provided with porous plate, and the decomposed compost of biology is laid on the porous plate.
6. a kind of experimental provision for laboratory cultures earthworm according to claim 5, it is characterised in that: the ventilation The both ends of the inwardly angled incubator of inner vent on plate are arranged, and the upper surface of the ventilating board is provided with ventilation groove, The ventilation groove is connected to each inner vent.
7. a kind of experimental provision for laboratory cultures earthworm according to claim 5, it is characterised in that: the collection Case includes collection tank, hook, hanging seat, baffle, climbs out of hole, wherein the one end of the interior incubator is provided with hanging seat, described The upper end of collection tank is provided with hook, and the collection tank is hung on the hanging seat using described, the collecting box Body it is open at one end close to the interior incubator, the collection tank is located on the interior incubator and is supported with the interior incubator Touching connection fragment position at be provided with it is multiple climb out of hole, the position for climbing out of hole on the inner wall of the interior incubator described in face can plug together Be provided with baffle.
8. a kind of experimental provision for laboratory cultures earthworm according to claim 1, it is characterised in that: described to drive Component further includes electromagnetic viscosimeter component, the electromagnetic viscosimeter component be located at the ventilating board and the interior incubator inner bottom wall it Between.
9. a kind of experimental provision for laboratory cultures earthworm according to claim 1, it is characterised in that: the interior training The bottom setting of case is supported there are two sliding block, the bottom of the outer incubator is provided with sliding slot, and the sliding block can on the sliding slot The setting slid into and out, the decomposed compost of biology include at least bottom spongy layer, top spongy layer and biological feed layer, wherein The bottom spongy layer, top spongy layer are located at the bottom and top of the biological feed layer, and the thickness of the top spongy layer is small In the bottom spongy layer.
10. a kind of experimental provision for laboratory cultures earthworm described in a kind of -9 any one according to claim 1 is answered With, it is characterised in that: it is applied to laboratory cultures earthworm, and carries out real-time monitoring and control to the culture parameters of earthworm, In, culture parameters include at least temperature sensor, humidity sensor and pH value sensor.
CN201910003065.4A 2019-01-02 2019-01-02 A kind of experimental provision and application for laboratory cultures earthworm Withdrawn CN109644948A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110950687A (en) * 2019-10-31 2020-04-03 安徽理工大学 Integrated automatic earthworm body fluid extraction device
CN112352742A (en) * 2020-11-10 2021-02-12 安徽理工大学 Intelligent earthworm coelomic fluid extraction system
CN112605120A (en) * 2020-12-25 2021-04-06 生态环境部南京环境科学研究所 Experimental device for be used for studying earthworm restoration lead zinc mining area heavy metal contaminated soil

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CN104026077A (en) * 2014-05-05 2014-09-10 辽宁华电环保科技有限公司 Method for cultivating earthworms
CN107372362A (en) * 2017-07-26 2017-11-24 江苏卓达信息科技有限公司 A kind of vermiculture bed for being easy to the later stage to separate
CN107683826A (en) * 2017-09-25 2018-02-13 南京律智诚专利技术开发有限公司 A kind of earthworm training bed automatically controlled
CN108040981A (en) * 2017-10-30 2018-05-18 聚德天禾农业科技(天津)有限公司 A kind of standardization box for breeding for earthworm breeding
CN108094333A (en) * 2017-12-19 2018-06-01 奥为(天津)环保科技有限公司 A kind of intensive style earthworm breeding device
CN108812555A (en) * 2018-06-27 2018-11-16 陈盛联 Utilize the method for kitchen garbage raising earthworm
CN208144234U (en) * 2018-04-12 2018-11-27 天津市忠涛蚯蚓养殖专业合作社 A kind of earthworm is collected and transport device

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104026077A (en) * 2014-05-05 2014-09-10 辽宁华电环保科技有限公司 Method for cultivating earthworms
CN107372362A (en) * 2017-07-26 2017-11-24 江苏卓达信息科技有限公司 A kind of vermiculture bed for being easy to the later stage to separate
CN107683826A (en) * 2017-09-25 2018-02-13 南京律智诚专利技术开发有限公司 A kind of earthworm training bed automatically controlled
CN108040981A (en) * 2017-10-30 2018-05-18 聚德天禾农业科技(天津)有限公司 A kind of standardization box for breeding for earthworm breeding
CN108094333A (en) * 2017-12-19 2018-06-01 奥为(天津)环保科技有限公司 A kind of intensive style earthworm breeding device
CN208144234U (en) * 2018-04-12 2018-11-27 天津市忠涛蚯蚓养殖专业合作社 A kind of earthworm is collected and transport device
CN108812555A (en) * 2018-06-27 2018-11-16 陈盛联 Utilize the method for kitchen garbage raising earthworm

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110950687A (en) * 2019-10-31 2020-04-03 安徽理工大学 Integrated automatic earthworm body fluid extraction device
CN112352742A (en) * 2020-11-10 2021-02-12 安徽理工大学 Intelligent earthworm coelomic fluid extraction system
CN112605120A (en) * 2020-12-25 2021-04-06 生态环境部南京环境科学研究所 Experimental device for be used for studying earthworm restoration lead zinc mining area heavy metal contaminated soil
CN112605120B (en) * 2020-12-25 2022-01-18 生态环境部南京环境科学研究所 Experimental device for be used for studying earthworm restoration lead zinc mining area heavy metal contaminated soil

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Application publication date: 20190419