CN109642221B - 新酯酶及其用途 - Google Patents

新酯酶及其用途 Download PDF

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Publication number
CN109642221B
CN109642221B CN201780051442.0A CN201780051442A CN109642221B CN 109642221 B CN109642221 B CN 109642221B CN 201780051442 A CN201780051442 A CN 201780051442A CN 109642221 B CN109642221 B CN 109642221B
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China
Prior art keywords
esterase
polyester
host cell
seq
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201780051442.0A
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English (en)
Chinese (zh)
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CN109642221A (zh
Inventor
V·图尼尔
H·特谢尔
M-L·德鲁索
C·托珀姆
I·安德烈
S·巴比
S·迪奎斯内
A·马蒂
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Carbios SA
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Carbios SA
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Publication date
Application filed by Carbios SA filed Critical Carbios SA
Priority to CN202210724621.9A priority Critical patent/CN114891768B/zh
Priority to CN202210723659.4A priority patent/CN114891766A/zh
Publication of CN109642221A publication Critical patent/CN109642221A/zh
Application granted granted Critical
Publication of CN109642221B publication Critical patent/CN109642221B/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01001—Carboxylesterase (3.1.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
  • Detergent Compositions (AREA)
CN201780051442.0A 2016-07-12 2017-07-12 新酯酶及其用途 Active CN109642221B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202210724621.9A CN114891768B (zh) 2016-07-12 2017-07-12 新酯酶及其用途
CN202210723659.4A CN114891766A (zh) 2016-07-12 2017-07-12 新酯酶及其用途

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16305899 2016-07-12
EP16305899.3 2016-07-12
PCT/EP2017/067577 WO2018011281A1 (en) 2016-07-12 2017-07-12 Novel esterases and uses thereof

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN202210724621.9A Division CN114891768B (zh) 2016-07-12 2017-07-12 新酯酶及其用途
CN202210723659.4A Division CN114891766A (zh) 2016-07-12 2017-07-12 新酯酶及其用途

Publications (2)

Publication Number Publication Date
CN109642221A CN109642221A (zh) 2019-04-16
CN109642221B true CN109642221B (zh) 2022-07-08

Family

ID=56511494

Family Applications (3)

Application Number Title Priority Date Filing Date
CN201780051442.0A Active CN109642221B (zh) 2016-07-12 2017-07-12 新酯酶及其用途
CN202210724621.9A Active CN114891768B (zh) 2016-07-12 2017-07-12 新酯酶及其用途
CN202210723659.4A Pending CN114891766A (zh) 2016-07-12 2017-07-12 新酯酶及其用途

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN202210724621.9A Active CN114891768B (zh) 2016-07-12 2017-07-12 新酯酶及其用途
CN202210723659.4A Pending CN114891766A (zh) 2016-07-12 2017-07-12 新酯酶及其用途

Country Status (8)

Country Link
US (2) US10590401B2 (https=)
EP (1) EP3485006B1 (https=)
JP (2) JP7189866B2 (https=)
KR (2) KR102685555B1 (https=)
CN (3) CN109642221B (https=)
CA (1) CA3030469C (https=)
MX (1) MX2019000493A (https=)
WO (1) WO2018011281A1 (https=)

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CN107709457B (zh) 2015-06-12 2021-05-25 卡比奥斯公司 可生物降解聚酯组合物及其用途
MX2018014090A (es) 2016-05-19 2019-04-01 Carbios Un proceso para degradar productos de plastico.
CA3030469C (en) * 2016-07-12 2024-04-30 Carbios Esterases and uses thereof
WO2018011284A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
BR112019012258A2 (pt) 2016-12-16 2020-10-20 Carbios proteases de degradação de plástico aprimoradas
DE102017214870A1 (de) * 2017-08-24 2019-03-14 Henkel Ag & Co. Kgaa Verbesserte Pflegeeigenschaften von Polyester Textilien II
JP7217267B2 (ja) 2017-08-31 2023-02-02 キャルビオス 生物学的実体を含む液体組成物及びその使用
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
DE102018210609A1 (de) * 2018-06-28 2020-01-02 Henkel Ag & Co. Kgaa Polyesterase II
US11692181B2 (en) * 2018-07-27 2023-07-04 Carbios Esterases and uses thereof
PT3830254T (pt) * 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações
JP7601756B2 (ja) * 2018-07-27 2024-12-17 キャルビオス 新規なエステラーゼ及びその使用
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
EP3699272A1 (en) 2019-02-21 2020-08-26 Henkel AG & Co. KGaA Improved caring properties on polyester textiles by use of a cutinase
CA3129549A1 (en) 2019-03-28 2020-10-01 Carbios Multicomponent thermoplastic product
JP7638952B2 (ja) * 2019-07-11 2025-03-04 キャルビオス 新規エステラーゼ及びその使用
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EP3838976B1 (en) 2019-12-19 2025-02-12 Carbios Process for degrading plastic products
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GB202005073D0 (en) 2020-04-06 2020-05-20 Mellizyme Biotechnology Ltd Enzymatic degradation of plastics
WO2022090290A1 (en) * 2020-10-27 2022-05-05 Carbios Novel esterases and uses thereof
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WO2022090288A1 (en) * 2020-10-27 2022-05-05 Carbios Novel esterases and uses thereof
CN117413007A (zh) 2021-05-20 2024-01-16 卡比奥斯公司 降解包含至少一种聚酯的塑料产品的方法
AU2022277645A1 (en) 2021-05-20 2023-11-30 Carbios Process for degrading a plastic product comprising at least one polyester
JP7732636B2 (ja) * 2021-10-13 2025-09-02 国立大学法人静岡大学 タンパク質、ポリヌクレオチド、組換えベクター、形質転換体、ポリエチレンテレフタレート分解用組成物、及びリサイクル品の製造方法
GB202207298D0 (en) * 2022-05-18 2022-06-29 Cambridge Entpr Ltd Improved plastic degrading enzymes
WO2023242197A1 (en) 2022-06-13 2023-12-21 Carbios Process for purifying mono-ethylene glycol
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
JPWO2024122208A1 (https=) * 2022-12-08 2024-06-13
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FR3144996A1 (fr) 2023-01-18 2024-07-19 Carbiolice Melange maitre enzyme comprenant des proteines
CN116286727B (zh) * 2023-03-06 2025-09-16 山东大学 一种角质酶变体及其制备方法和在塑料降解中的应用
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TW202511480A (zh) 2023-05-15 2025-03-16 法商卡爾畢歐斯公司 新穎之酯酶及其用途
TW202509131A (zh) 2023-07-10 2025-03-01 法商卡爾畢歐斯公司 紡織廢料之預處理方法
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BR112019012258A2 (pt) 2016-12-16 2020-10-20 Carbios proteases de degradação de plástico aprimoradas

Also Published As

Publication number Publication date
EP3485006A1 (en) 2019-05-22
CN109642221A (zh) 2019-04-16
CA3030469C (en) 2024-04-30
JP2019527060A (ja) 2019-09-26
JP7189866B2 (ja) 2022-12-14
CN114891768B (zh) 2024-09-24
CN114891768A (zh) 2022-08-12
US11072784B2 (en) 2021-07-27
KR20230011505A (ko) 2023-01-20
JP2022101632A (ja) 2022-07-06
US10590401B2 (en) 2020-03-17
KR20190028754A (ko) 2019-03-19
KR102489444B1 (ko) 2023-01-20
US20200277585A1 (en) 2020-09-03
US20190225954A1 (en) 2019-07-25
EP3485006B1 (en) 2025-10-29
JP7392029B2 (ja) 2023-12-05
MX2019000493A (es) 2019-04-01
KR102685555B1 (ko) 2024-07-17
CN114891766A (zh) 2022-08-12
CA3030469A1 (en) 2018-01-18
EP3485006C0 (en) 2025-10-29
WO2018011281A1 (en) 2018-01-18

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