CN109607815A - Utilize the method for heavy metal in spindle lysine bacillus mineralized waste incineration bottom ash leachate - Google Patents
Utilize the method for heavy metal in spindle lysine bacillus mineralized waste incineration bottom ash leachate Download PDFInfo
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- CN109607815A CN109607815A CN201811487978.XA CN201811487978A CN109607815A CN 109607815 A CN109607815 A CN 109607815A CN 201811487978 A CN201811487978 A CN 201811487978A CN 109607815 A CN109607815 A CN 109607815A
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- Prior art keywords
- bottom ash
- waste incineration
- incineration bottom
- ash leachate
- lysine bacillus
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/103—Arsenic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
Abstract
The invention discloses a kind of methods using heavy metal in spindle lysine bacillus mineralized waste incineration bottom ash leachate, spindle lysine bacillus is inoculated in the waste incineration bottom ash leachate containing urea and calcium chloride, the shaken cultivation in 20-30 DEG C, the shaking table of 100-150rpm realizes the mineralising to heavy metal in waste incineration bottom ash leachate.The present invention has many advantages, such as that simple process, easy to operate, effect is obvious, processing cost is low, applied widely and without secondary pollution by heavy metal of spindle lysine bacillus under conditions of urea and calcium chloride in repair process waste incineration bottom ash leachate.Leachate after reparation is cleared up with ICP-OES analysis shows that the removal rate of Pb, Cd and As are respectively 96.0%, 98.9%, 92.8%.
Description
(1) technical field
The present invention relates to a kind of sides using spindle lysine bacillus mineralising processing waste incineration bottom ash leachate
Method belongs to environmental protection field of solid waste treating technology.
(2) background technique
With the development of Chinese society and perfect, from the last century 80's, government is also dedicated to consumer waste incineration
The construction of factory, until 2012 end of the years, the whole nation is completed 213 garbage burning factories, and garbage burning factory day processing rubbish ability is
215844t/d.Contain various heavy metal components (such as cadmium, lead, arsenic etc.) in bottom ash caused by waste incineration.The rubbish largely stored up
Rubbish incineration bottom ash can generate a large amount of leachate containing heavy metal under the natural conditions such as rainfall, to cause serious secondary dirt
Dye.
Lead is a kind of common heavy metal, toxic.The long-term persistence of lead in the environment, but to many vital tissues have compared with
Strong potentiality toxicity, so lead is listed in always strong pollutant range.At very low concentrations, the chronic long health effect of lead
It should show themselves in that influence brain and nervous system.After lead invades nervous system, there is insomnia, dreaminess, hypomnesia, tired, in turn
Development is manic, blindness, in a certain state of delirium, stupor, finally death due to cerebrovascular anoxic.It is micro- that Pb-B is often higher than 2.16
When mol/L, it just will appear clinical symptoms, therefore Pb-B is although higher in many children's bodies, but particularly not
Suitable, the change on mild mental or behavior is also difficult to be found by parent or doctor.This is also why children's lead poisoning is in foreign countries
The reason of referred to as " hiding killer ".In addition, lead also has carcinogenic, teratogenesis and mutagenic effect.Heavy metal cadmium has incubation period
The features such as length, toxicity are high, difficult to degrade, ranking the 7th in 20 kinds of elements for seriously threatening human health are a kind of strong carcinogenic
Heavy metal element.The cardinal symptom of cadmium poisoning is anaemia, nervous function imbalance and injury of kidney.The compound of arsenic has trivalent arsenic and five
Valence arsenic, wherein the toxicity of trivalent arsenic compound be much larger than pentavalent arsenic toxicity, about 60 times.Water, food and air of the arsenic through polluting
After entering human body, according to enter human body number can cause acute or chronic arsenic poisoning.Acute arsenic poisoning: Report is more common in alimentary canal,
It is mainly shown as that the vomiting occurred immediately, esophagus, abdominal pain bleeding and bloody stool etc., rescue can cause death not in time.
Meanwhile arsenic can cause cutaneum carcinoma, lung cancer, bladder cancer and kidney.
Current main heavy metal leachate restorative procedure has an electroosmose process, heavy metal flocculant method, oxidation-reduction method etc.,
There is poor removal effects for these physico-chemical process, at high cost, and be easy to cause secondary pollution problems.
Spindle lysine bacillus is a kind of urease-producing bacterium, and urease-producing bacterium can secrete urase, and urase hydrolyzes urea meeting
Form CO3 2-And NH4 +Ion, while keeping the pH of environment significantly raised, under conditions of meta-alkalescence, heavy metal ion a part with
Carbanion forms precipitating, and another part heavy metal can be precipitated in the form of co-precipitation.Currently, what is had been reported utilizes production urea
Enzyme bacterium processing heavy metal technique is mainly the heavy metal in microorganism curing soil, but utilizes urease-producing bacterium mineralising repair process rubbish
Rubbish incineration bottom ash leachate has not been reported.
(3) summary of the invention
The mineralized waste incineration bottom ash leaching of spindle lysine bacillus is utilized the purpose of the present invention is to provide a kind of
The method of heavy metal in liquid solves heavy metal in leachate produced by causing the waste incineration bottom ash of stockpiling as natural precipitation etc.
The problem of to soil and underground water pollution.
Technical scheme is as follows:
The present invention, which provides, a kind of utilizes heavy metal in spindle lysine bacillus mineralized waste incineration bottom ash leachate
Method, the method are as follows: by spindle lysine bacillus be inoculated with the waste incineration bottom ash leaching containing urea and calcium chloride
In liquid, shaken cultivation (preferably 30 DEG C, 130rpm cultivate 2 days), is realized to rubbish in 20-30 DEG C, the shaking table of 100-150rpm
The mineralising of heavy metal in incineration bottom ash leachate.
Further, spindle lysine bacillus of the present invention be preferably purchased from BeiJing, China north receive biological inoculum preservation
Library, microorganism resource number BNCC210664.
Further, spindle lysine bacillus of the present invention first carries out enrichment culture before inoculation, then will enrichment
Culture bacterium solution is seeded in waste incineration bottom ash leachate to OD600For 0.8~1.2 (preferably 1.0), the Enrichment culture method
Are as follows: spindle lysine bacillus is seeded in nutrient broth medium, in 25~35 DEG C, 100~150rpm (preferably 30
DEG C, 130rpm) shaking table in cultivate 8~16h, obtain bacterium solution；The nutrient broth medium composition are as follows: peptone 10g/L, ferment
Mother leaching powder 1.5g/L, beef extract 1.5g/L, sodium chloride 5g/L, solvent be waste incineration bottom ash leachate, pH4.0 ± 0.1~
12.0 ± 0.1, preferably pH8.5 ± 0.1.
Further, in waste incineration bottom ash leachate of the present invention heavy metal Pb, Cd and As concentration be respectively 17.50 ±
0.50mg/kg、6.00±0.20mg/kg、13.00±0.50mg/kg。
Further, the concentration of urea is 10-100g/L, preferably 15~25g/L in waste incineration bottom ash leachate of the present invention
(more preferable 20g/L), the concentration of calcium chloride are 10-100mmol/L, preferably 20~30mmol/L (more preferable 25mmol/L).
Waste incineration bottom ash leachate of the present invention is to be drenched with the waste incineration bottom ash of 5g:1ml solid water ratio with tap water
It filters, wherein waste incineration bottom ash is derived from garbage incinerating power plant, and garbage incinerating power plant is by special burning boiler
Combusting Municipal Solid rubbish, then a kind of forms of electricity generation to be generated electricity by steam turbine generator group.Wherein boiler combustion solid garbage
Bottom ash caused by after body waste is heretofore described waste incineration bottom ash, mainly contains heavy metal lead Pb, cadmium Cd
With arsenic As.
The present invention using BeiJing, China north receive biological inoculum save library buy one plant of urease-producing bacterium --- spindle relies ammonia
Sour bacillus (number BNCC210664), mineralising repair waste incineration bottom ash leachate and rely spindle under aerobic condition
Propylhomoserin bacillus is seeded in the nutrient broth medium being made by waste incineration bottom ash leachate, and urine is added in bacterium solution
Element and calcium chloride calcification 2-3 days, complete the mineralising of heavy metal in waste incineration bottom ash leachate under the conditions of 20~30 DEG C
Process.Carbonate sediment and part heavy metal and calcium carbonate are generated under conditions of urea and calcium chloride by urease-producing bacterium
Co-precipitation is generated, so that mineralising repairs the heavy metal in waste incineration bottom ash leachate.
Compared with prior art, the beneficial effects are mainly reflected as follows: by the leachate after reparation through resolution use
ICP-OES is analysis shows that the removal rate of Pb, Cd and As are respectively 96.0%, 98.9%, 92.8%.
The present invention passes through repair process waste incineration under conditions of urea and calcium chloride of spindle lysine bacillus
Heavy metal in bottom ash leachate, with simple process, easy to operate, effect is obvious, processing cost is low, applied widely and nothing
The advantages that secondary pollution.
(4) Detailed description of the invention
Fig. 1 is life of the 1 spindle lysine bacillus of embodiment in the waste incineration bottom ash leachate of various concentration
Long curve.
(5) specific embodiment
Below by specific embodiment, the present invention is further illustrated, but protection scope of the present invention is not limited in
This.
The spindle lysine bacillus used in embodiment purchased from BeiJing, China north receive biological inoculum save library, strain
Resource number BNCC210664.
Waste incineration bottom ash is derived from Shaoxing, Zhejiang Province city, obtains waste incineration bottom ash leachate by solid water ratio 5g:1ml,
After measured in waste incineration bottom ash leachate heavy metal Pb, Cd and As concentration be respectively 17.98mg/kg, 5.98mg/kg,
13.31mg/kg。
Adaptation Journal of Sex Research nutrient broth of the 1 spindle lysine bacillus of embodiment in waste incineration bottom ash leachate
Culture medium composition are as follows: peptone 10g/L, yeast extract 1.5g/L, beef extract 1.5g/L, sodium chloride 5g/L, solvent burn for rubbish
It burns bottom ash leachate to be made into deionized water, pH8.5 ± 0.1.
Spindle lysine bacillus is seeded to by waste incineration bottom ash leachate and deionized water by different volumes
It is made into the nutrient broth medium of solvent preparation, the bulk concentration gradient of waste incineration bottom ash leachate in solvent are as follows: 0%,
1%, 2.5%, 5%, 10%, 25%, 50%, 100%.The overnight incubation (12h) in 30 DEG C, the shaking table of 130rpm, passes through spinning
Growth curve of the capitate lysine bacillus in the waste incineration bottom ash leachate of various concentration (uses OD600Indicate) see Fig. 1
It is shown, the results showed that, spindle lysine bacillus can be with normal growth in waste incineration bottom ash leachate.
2 spindle lysine bacillus mineralized waste incineration bottom ash leachate of embodiment
(1) enrichment culture is carried out to spindle lysine bacillus
Spindle lysine bacillus is seeded to the nutrient meat being made by waste incineration bottom ash leachate as solvent
In soup culture medium, the overnight incubation (12h) in 30 DEG C, the shaking table of 130rpm obtains bacterium solution, passes through waste incineration bottom ash leachate
Dilution adjusts bacterium solution OD600It is 1.0；
The formula of nutrient broth medium are as follows: 1L waste incineration bottom ash leachate, 10g peptone, 1.5g yeast extract,
1.5g beef extract and 5g sodium chloride, pH are 8.5 ± 0.1.
(2) spindle lysine bacillus mineralized waste incineration bottom ash leachate is used
In the bacterium solution obtained to 1000ml step (1), 20g urea, 2.776g (25mmol) calcium chloride is added, is contained
Then the bacterium solution of 20g/L urea and 25mM calcium chloride vibrates bacterium solution 2 days in 30 DEG C, the shaking table of 130rpm, after being repaired
Bacterium solution.By the bacterium solution centrifugal filtration after reparation, measured with the analysis that ICP-OES carries out lead Pb, cadmium Cd and arsenic As, as the result is shown
The removal rate of Pb, Cd and As are respectively 96.0%, 98.9%, 92.8%.
Comparative example 1
Spindle lysine bacillus in embodiment 2 is replaced with to the Staphylococcus cohnis solution urea Asia that can produce urase
Kind of (bacterium receives biological inoculum purchased from BeiJing, China north and saves library, microorganism resource number BNCC337537), other same embodiments of operation
2, when the bacterium being selected to carry out mineralising reparation, tested by growth curve, bacterium solution OD600=0, which is difficult in waste incineration bottom ash
Normal growth in leachate.
Comparative example 2
Urea in embodiment 2 and calcium chloride are removed, other same, the removal rate of Pb, Cd and As are respectively as the result is shown
18.2%, 28.9%, 9.9%.
Embodiment 3
PH in experimental example 2 is changed to 4.0 ± 0.1,12.0 ± 0.1, other operations are the same as embodiment 2, the leaching of waste incineration bottom ash
The removal rate of Pb, Cd and As are shown in Table 1 in filtrate.
The heavy metal removing rate of difference pH in the reparation of 1 spindle lysine bacillus mineralising of table
Embodiment 4
The concentration of urea in embodiment 2 is changed to 10,20,50 and 100g/L respectively, other operations are the same as embodiment 2, rubbish
The removal rate of Pb, Cd and As are shown in Table 2 in incineration bottom ash leachate.
The heavy metal removing rate of different urea concentrations in the reparation of 2 spindle lysine bacillus mineralising of table
Embodiment 5
By when calcium chloride concentration is changed to 10mM, 25mM, 50mM and 100mM in embodiment 2, other operations are the same as embodiment 2, rubbish
The removal rate of Pb, Cd and As are shown in Table 3 in rubbish incineration bottom ash leachate.
The heavy metal removing rate of different calcium chloride concentrations in the reparation of 3 spindle lysine bacillus mineralising of table
Claims (7)
1. a kind of method using heavy metal in spindle lysine bacillus mineralized waste incineration bottom ash leachate, feature
It is the method are as follows: spindle lysine bacillus is inoculated with the waste incineration bottom ash leachate containing urea and calcium chloride
In, the shaken cultivation in 20-30 DEG C, the shaking table of 100-150rpm realizes the mine to heavy metal in waste incineration bottom ash leachate
Change.
2. the method as described in claim 1, it is characterised in that the spindle lysine bacillus is BeiJing, China Bei Na
The spindle lysine bacillus of biological inoculum preservation library number BNCC210664.
3. the method as described in claim 1, it is characterised in that the spindle lysine bacillus is inoculated with OD600For 0.8~
1.2。
4. the method as described in claim 1, it is characterised in that the concentration of urea is 10 in the waste incineration bottom ash leachate
~100g/L, the concentration of calcium chloride are 10~100mmol/L.
5. the method as described in claim 1, it is characterised in that the concentration of urea is in the waste incineration bottom ash leachate
20g/L, the concentration of calcium chloride are 25mmol/L.
6. the method as described in claim 1, it is characterised in that the spindle lysine bacillus first carries out before inoculation
Enrichment culture, then enrichment culture bacterium solution is seeded in waste incineration bottom ash leachate to OD600It is 0.8~1.2, the enrichment
Cultural method are as follows: spindle lysine bacillus is seeded in nutrient broth medium, 25~35 DEG C, 100~
8~16h is cultivated in the shaking table of 150rpm, obtains bacterium solution；The nutrient broth medium composition are as follows: peptone 10g/L, yeast leaching
Powder 1.5g/L, beef extract 1.5g/L, sodium chloride 5g/L, solvent be waste incineration bottom ash leachate, pH4.0 ± 0.1~12.0 ±
0.1。
7. the method as described in claim 1, it is characterised in that heavy metal Pb, Cd and As in the waste incineration bottom ash leachate
Concentration is respectively 17.50 ± 0.50mg/kg, 6.00 ± 0.20mg/kg, 13.00 ± 0.50mg/kg.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110054444A (en) * | 2019-04-30 | 2019-07-26 | 东南大学 | It is a kind of suitable for the cement-based material self repairing agent of low temperature environment and its application |
| CN111534689A (en) * | 2020-03-20 | 2020-08-14 | 浙江工业大学 | Method for mineralizing and fixing heavy metal in tailings by utilizing fusiform lysine bacillus and quicklime |
| CN114292882A (en) * | 2022-01-06 | 2022-04-08 | 兰州大学 | Method for promoting microorganism to induce carbonate precipitation by using attapulgite clay |
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Cited By (3)
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| CN114292882A (en) * | 2022-01-06 | 2022-04-08 | 兰州大学 | Method for promoting microorganism to induce carbonate precipitation by using attapulgite clay |
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