CN109607789A - Efficiently nitrogen-cycle bacteria is ecological bed - Google Patents
Efficiently nitrogen-cycle bacteria is ecological bed Download PDFInfo
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- CN109607789A CN109607789A CN201811642193.5A CN201811642193A CN109607789A CN 109607789 A CN109607789 A CN 109607789A CN 201811642193 A CN201811642193 A CN 201811642193A CN 109607789 A CN109607789 A CN 109607789A
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- nitrogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Biological Treatment Of Waste Water (AREA)
Abstract
The invention belongs to ecological protection fields; pass through the ecological bed of efficient nitrogen-cycle bacteria; in the conversion zone that ecological bed interior formation is stable, overcomes conventional art to fail or be forced substantially the shortcomings that stopping in autumn and winter, realize the continuous operation of ecological engineering at a lower temperature.By the effect of nitrobacteria and denitrifying bacteria, make that natural water, sanitary sewage, the nitrogen of various forms is gradually removed according to nitrogen cycle reaction in industrial wastewater, has a significant effect to nitrogen removal.
Description
Technical field
The invention belongs to ecological engineering of environment technical fields, are related to microorganism artificial screening and its answering in environmental improvement
With more specifically, being that efficient nitrogen-cycle bacteria is ecological bed.
Background technique
Lake, the pollution of the water bodys such as river and eutrophication are Chinese or even human health is jeopardized in many areas in the world,
Restrict the severe ecological environment problem of Sustainable Socioeconomic Development.So far existing research shows that causing water eutrophication
All kinds of nutriments in, nitrogen, phosphorus are main limitation sex factors.Therefore solve the problems, such as one of key of water eutrophication just
It is to control the content of the nitrogen of water body.
The most of improvement research and application both domestic and external at present for sanitary sewage and industrial wastewater are biological denitrificaions
Method, but nitrification-denitrification process is difficult to unify over time and space, denitrification effect is poor, causes this multistep of biological denitrificaion
Rapid biocatalytic reaction is limited by matrix delivery rate, substrate and Product inhibiton etc..Nitrification needs nitrobacteria, wherein nitre
Changing bacterium includes nitrite bacteria and nitrate bacteria, and nitrification must could be completed by the collective effect of these two types of bacteriums,
Single bacterium cannot complete, but two class bacteriums will lead to the reduction of nitrification in varying environment, therefore need two class bacteriums
Rong He Dao Cu.
Summary of the invention
It is followed in view of the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is that providing a kind of efficient nitrogen
Ring bacteria artificial screening.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: a kind of efficient nitrogen-cycle bacteria is ecological bed, packet
The fixation device of efficient nitrogen-cycle bacteria release, ecological floating island and the fixed ecological floating island is included, the ecological floating island includes
Multiple PVC sticks, the edge of the PVC stick are equipped with evagination buckle and indent locked groove, and PVC stick one end center offers circle
Hole, another end surfaces of the PVC stick are equipped with boss, and the boss center is equipped with the regular polygon for penetrating the round hole
Pipe, the regular polygon pipe are located at the boss one end open, other end closing, and the efficient nitrogen-cycle bacteria release is fixed
It is set to regular polygon pipe open end, the round hole is fixedly connected with the regular polygon pipe by multiple connecting plates,
The connecting plate and the regular polygon pipe will be divided into multiple exhalant regions, the regular polygon Guan Shangshe inside the round hole
There are multiple limbers, the water in the regular polygon pipe is flowed into the multiple exhalant region by the multiple limbers, and from
Other end outflow.
Preferably, the evagination buckle and the indent locked groove are disposed adjacent.
Preferably, the evagination buckle and the indent locked groove are symmetrical arranged.
Preferably, the projection shape is regular hexagon.
Preferably, the projection shape is circle.
Preferably, the fixed device include the sliding slot that river both sides are set, it is more above river across being arranged in
A crossbeam, the crossbeam move along the chute, and suspention is equipped with the ecological floating island on two adjacent crossbeams.
Preferably, the ecological floating island Corner Strapped is connected with sunpender, and the sunpender is height-adjustable screw rod,
The upper end of the sunpender is provided with idler wheel, and the idler wheel is arranged on the crossbeam, and along the cross beam movement, the sunpender
Upper end, which is provided with, pulls crossbeam.
The beneficial effects of the present invention are: the present invention passes through the ecological bed of the efficient nitrogen-cycle bacteria of reform first, by inhalant region
It is arranged to roomy regular hexagon pipe, in this way the inflation of guarantee oxygen, better conforms to bacterial nitrification reaction, water passes through regular hexagon pipe
On limbers flow into narrow exhalant region, form anaerobic zone, ensure that the anti-nitration reaction of bacterium, in this way setting sufficiently decomposition
Nitrogen in river water；The present invention, which also passes through, improves bacteria selection and enrichment method, and obtaining efficiencies of nitrogen removal, preferably efficient nitrogen follows
Ring bacterium.These efficient nitrogen-cycle bacterias are applied to sanitary sewage, the improvement of industrial wastewater and the life to eutrophication water
When state is repaired, ecological engineering-immobilization technology is enhanced to the tolerance of adverse circumstances, overcomes conventional art in autumn and winter
Substantially it fails or is forced the shortcomings that stopping, realizing the continuous operation of ecological engineering at a lower temperature
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is the structural schematic diagram that device is fixed in the present invention；
Fig. 3 is that ecological floating island is located at the top view of river water in a case study on implementation in the present invention；
Fig. 4 is one end structural schematic diagram of PVC stick in a case study on implementation in the present invention；
Fig. 5 is the other end structural schematic diagram of PVC stick in a case study on implementation in the present invention；
Fig. 6 is that ecological floating island is located at the top view of river water in another case study on implementation in the present invention；
Fig. 7 is one end structural schematic diagram of PVC stick in another case study on implementation in the present invention；
Fig. 8 is the other end structural schematic diagram of PVC stick in another case study on implementation in the present invention.
Description of symbols:
1-ecological floating island；2-fixed devices；3-sliding slots；4-crossbeams；5-idler wheels；6-sunpenders；7-pull crossbeam 7；
8-PVC sticks；9-high nitrogen-cycle bacteria releases；81-evaginations buckle；82-indent locked grooves；83-round holes；84-is just more
Side shape pipe；85-connecting plates；86-exhalant regions；87-limbers；88-boss；81a-evagination buckle；82a-indent locked groove；
83a-round hole；84a-regular polygon pipe；85a-connecting plate；86a-exhalant region；87a-limbers；88a-boss.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein are not precluded one or more
The presence or addition of a other elements or combinations thereof.
As shown in Figs. 1-2, the present embodiment provides a kind of the ecological bed of efficient nitrogen-cycle bacteria, including efficient nitrogen-cycle bacteria
The fixation device 2 of release 9, ecological floating island 1 and fixed ecological floating island, fixed device 2 include the sliding slot that river both sides are arranged in
3, across the multiple crossbeams 4 being arranged in above river, the crossbeam 43 moves along the chute, suspends in midair and set on two adjacent crossbeams 4
There is ecological floating island 1,1 Corner Strapped of ecological floating island is connected with sunpender 6, and sunpender 6 is height-adjustable screw rod, the upper end of sunpender 6
It is provided with idler wheel 5, the setting of idler wheel 5 is moved on crossbeam 4, and along crossbeam 4, and 6 upper end of sunpender, which is provided with, pulls crossbeam 7, sets in this way
Meter can according to need puts multiple groups ecological floating island 1 in river, and sunpender 6 can change in the position of korneforos, also conveniently take
It puts.
As shown in figures 3-8, ecological floating island 1 includes multiple PVC sticks 8, and the edge of PVC stick 8 is equipped with evagination buckle 81 and indent
Locked groove 82,8 one end center of PVC stick offer round hole 83, and another end surfaces of PVC stick 8 are equipped with boss 88, and 88 center of boss is set
There is the regular polygon pipe 84 for penetrating round hole 83, regular polygon pipe 84 is located at 88 one end open of boss, and other end closing is high
Effect nitrogen-cycle bacteria release 9 is fixedly installed on 84 open end of regular polygon pipe, and round hole 83 and regular polygon pipe 84 pass through more
A connecting plate 85 is fixedly connected, and connecting plate 85 and regular polygon pipe 84 will be divided into multiple exhalant regions 86 inside round hole 83, just
Polygonal tube 84 is equipped with multiple limbers 87, and the water in regular polygon pipe 84 flows into multiple exhalant regions by multiple limbers 87
It in 86, designs in this way, bacterium is discharged by the water inlet that efficient nitrogen-cycle bacteria release 9 is set to PVC stick 8 by river water
In PVC stick 8, such bacterium is attached in PVC stick 8, can form stable aerobic zone and anaerobic zone, is sufficiently decomposed in water
Nitrogen realizes that river water maximizes purification.
Embodiment 1
Evagination buckle 81 and indent locked groove 82 are disposed adjacent, and shape is adapted, and design in this way does not have to distinguish PVC stick 8
Direction can just think and quickly be spliced into ecological floating island 1, and 88 shape of boss is regular hexagon, in this way with the cross section shape of PVC stick 8
Shape is the same, and processing does not have to replacement program, facilitates processing, and increase boss 88 can distinguish direction into the water.
Embodiment 2
Evagination buckles 81a and indent locked groove 82a and is symmetrical arranged, and shape is adapted, and design is so that splicing ecology is floating in this way
Island 1 is spliced in one direction, and when PVC stick 8 damages, the position of damage, boss 88a shape can be quickly found along stitching direction
Shape is circle, and circular design does not have corner angle, it is not easy to tangle water pollutant, will not block water inlet in this way.
As seen in figures 3-6, wherein regular polygon pipe is positive hexagonal tube, but regular polygon pipe can not only be positive
Hexagonal tube further includes other all regular polygon pipes.
The water inlet of regular hexagon pipe 84 is equipped with efficient nitrogen-cycle bacteria release device, and efficient nitrogen-cycle bacteria manually sieves
Select step:
Acquire bacterium water sample:
The water surface or less 10~20cm water layer is protruded into sterilized sterile test tube (1 × 1.5cm) in the water bodys such as lake to take
Sample seals immediately after sampling, to be seeded in laboratory.
Culture medium:
The culture medium of nitrite bacteria: (NH4)2SO42.0g, NaHPO4 0.25g, MnSO4·4H2O0.01g, K2HPO4
0.75g, MgSO4·7H27.2 121 DEG C of sterilizing 30min of O 0.03g, CaCO3 1.0g, distilled water 1000ml, pH.
The culture medium of nitrate bacteria: NaNO21.0g, Na2CO31.0g, NaHPO4 0.25g, CaCO3 1.0g, K2HPO4
0.75g, MnSO4 0.01g,MgSO4·4H27.2 121 DEG C of sterilizing 30min of O 0.03g, distilled water 1000ml, pH.
Denitrifying bacteria culture medium:
KNO32.0g, MgSO40.2g, KHSO40.5g, NaKC4H4O6·4H2O, distilled water 1000ml, pH 7.2
121 DEG C of sterilizing 30min.
Separation method:
(1) enrichment culture
Sample collected access is sterilized in advance, in the triangular flask of built-in bead and 90ml culture medium, in 17 DEG C
Shaking table 150r/min is cultivated 10 days.Purpose is to break up zoogloea, is dispersed in water bacterium at unicellular, while making mud sample
In nitrobacteria quantity increase.
(2) plating dilutions separate
From being taken out in test tube of the 1mL bacteria suspension access equipped with 9mL sterile water in enrichment culture bacteria suspension, 10 are obtained-1Gradient
Bacteria suspension, according to the above method available 10-2、10-3、10-4... the bacteria suspension of gradient chooses gradient 10-4—10-8Bacterium it is outstanding
Liquid, takes 1mL bacteria suspension to be put into plate, and culture medium appropriate is then added and mixes, and is inverted plate after culture medium solidification, is put into 17
7-14d is cultivated in DEG C incubator.After forming bacterium colony on culture medium to bacterium individual cells or cell lineage, single bacterium is taken
It falls within and is cultivated on slant medium, marked, in case subsequent use.
(3) plate streaking separates
From the lawn of the slant medium of the strain after dilution separation choose it is a little carry out plate streaking separation, so as into one
Step purifying.Whole process is required to sterile working.
The bacterium that picking is grown on a small quantity first from slant medium, then in the flat lining out made in advance.When scribing line
It is drawn as far as possible in the range of drawing again completely, then by oese calcination, is rotated further by certain angle and connects the region crossed again
Another area is drawn, completely entire plate is finally drawn, is put into 17 DEG C of incubators and cultivates 7-14d, obtain single colonie.Different bacterium colonies is turned
It is connected on inclined-plane, single plant bacterial strain can be obtained after being repeated several times, move into inclined-plane and give over to subsequent experimental use.
(4) separating resulting
According to the method described above, 42 plants of pure bacterial strains are isolated from nitrite bacteria culture medium, is separated from nitrate bacteria culture medium
45 plants of pure bacterial strains out isolate 27 plants of pure bacterial strains from denitrifying bacteria culture medium.
The Protoplast fusion of nitrite bacteria and nitrate bacteria
1. using the nitrite bacteria with selective genetic marker as parent.
Cell wall is removed through lysozyme 2. preparing protoplast, protoplast is released, maintains it in the hypertonic transparent liquid of juxtaposition
Stablize.
3. promoting fusion polyethylene glycol is added to protoplast to promote to merge, polyethylene glycol is a kind of surfactant, energy
Enforceable promotion protoplast fusion can more promote to merge in the presence of having Ca2+, Mg2+ ion.
4. protoplast regeneration protoplast has lost cell wall, though there is bioactivity, do not given birth on ordinary culture medium
It is long, it is necessary to be coated on regeneration culture medium, be allowed to regenerate.
5. detecting fusant using the genetic marker on Selective agar medium, it is determined whether be fusant.
6. the type that may have heterozygosis amphiploid different with single times of recombinant in the fusant that fusant screening generates, the former
Performance is unstable, to select single times of stable recombinant of performance, and repeated screening, which goes out, produces fusant of good performance.
Efficient immobilized nitrogen cycle bacteria (ammonification-nitrification-nitrosation-denitrification) is applied to the step of the current waters such as river
It is rapid:
(h) the efficient nitrogen-cycle bacteria for obtaining efficient nitrogen-cycle bacteria artificial screening described in claim 1 carries out rich
Collection；
(j) it by the blending decision after enrichment and after the preferred bacterium of denitrifying bacteria mixes, puts into the carrier prepared and is fixed
Change；
(k) wastewater treatment or general will be carried out in the efficient nitrogen-cycle bacteria investment wastewater treatment equipment of the immobilization prepared
The efficient nitrogen-cycle bacteria of immobilization is put into natural water and carries out restoration of the ecosystem to natural water.
Ecological floating island can be laid in step (j) in natural water, the high nitrogen cycle that ecological floating island hangs immobilization is thin
Immobilized nitrogen cycle bacteria carrier is placed in the container with mesh by bacterium release, the release, so that the height of immobilization
Effect circulation bacterium can contact water body, so that immobilized nitrogen cycle bacteria is discharged into natural water in this way, it is right
Natural water carries out restoration of the ecosystem.
Specific embodiment:
A. to be seeded in natural water body aquatic plants growth root zone water sampling
B. bacterium, denitrifying bacteria are merged by what above-mentioned artificial screening obtained efficient nitrate bacteria and nitrite bacteria.
C. two kinds of nitrogen-cycle bacterias are mixed in investment carrier, efficient immobilized nitrogen cycle bacteria is made.
D. the periphery natural environment of water body is investigated with pollution sources, determines the fluidised form and influent quality of neighbouring water body,
It determines the specific distribution of Project Areas, and starts to carry out the monitoring of Project Areas water quality.
E. ecological engineering test block is established in water body, check plot, immobilized nitrogen cycle bacteria test block are divided into experiment,
20kg immobilized nitrogen cycle bacteria is divided into 5kg, 5kg, 5kg by water plant+immobilized nitrogen cycle bacteria test block, and 5kg tetra-
Point, immobilized nitrogen cycle bacteria test block is arranged in ecological floating island 1 respectively and water plant+immobilized nitrogen cycle bacteria is tested
The both ends in area, each ecological floating island 1 hang an immobilized nitrogen cycle bacteria release, and the release is by immobilization nitrogen cycle
Bacteria carrier is placed in the special container with mesh, is placed under the water surface at 20~30cm, so that immobilization bacterium
Water body can be contacted.
F. the release of nitrogen-cycle bacteria increases the nitrogen-cycle bacteria quantity in unit volume water body in carrier, and tentatively increases
Nitrification-denitrification interface in water body enhances the Nitrogen releasing activity of ecological engineering trial zone water body, to introduce a fine variety in next step
Aquatic higher plants create condition.
G. monitoring works area water quality, when determining that having been able to guarantee aquatic higher plants grows, to artificial floating island
Introduce a fine variety aquatic higher plants.Aquatic higher plants are introduced a fine variety, and are made to increase many rhizosphere microecological systems in water body, are enhanced nitrogen
Recycle the treatment effect of bacterium.And the release of immobilized nitrogen cycle bacteria, and obviously increase aquatic higher plants rhizosphere bacteria；
And as the movement of water flow, nitrogen-cycle bacteria are further downstream spread, promotes the nitrogen of downstream water to spread, enhance full basin
Self-purification capacity.
Application effect:
There is apparent influence to water plant root zone bacterial number in trial zone using this method release nitrogen-cycle bacteria, it is raw
Denitrifying bacteria, nitrobacteria (fusion of nitrate bacteria and nitrite bacteria) quantity of water plant root zone in state chinampa 1
Between 4 to 6 orders of magnitude, hence it is evident that higher than the water plant root zone bacterial population in no ecological floating island 1,1~2 number of mean height
Magnitude, wherein denitrifying bacteria, nitrobacteria MPN value reach extremely significant horizontal (P < 0.01)；F inspection result shows anti-at water outlet
Nitrobacteria MPN value is extremely significant to be higher than MPN value (P < 0.01) at water inlet；Variance analysis show upper spacious pool, lower spacious pool,
At immobilized nitrogen cycle bacteria 10cm, 30cm；The nitrobacteria number of release in trial zone everywhere obviously higher than water inlet at,
Wherein away from the nitrobacteria number highest at immobilization nitrogen cycle bacteria artificial floating island 10cm, MPN value is extremely significant to be higher than (P at water inlet
< 0.001).During test, nitrogen-cycle bacteria number is also apparently higher than into Shui Chu in the waters of downstream.
After immobilized nitrogen cycle bacteria is administered, test block total nitrogen, nitrite nitrogen, non-ionic ammonia respectively than engineering before
Be substantially reduced, TN, NO3-, NH3 after peak 4.613mg/L, 0.075mg/L, 0.15mg/L engineering before engineering respectively under
It is reduced to 0.21mg/L, 0.008mg/L and 0.05mg/L, is shown using efficient immobilized nitrogen cycle bacteria technology to applied to river
The nitrogen removal of the current waters such as stream has a significant effect.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside", " up time
The orientation or positional relationship of the instructions such as needle ", " counterclockwise ", " axial direction ", " radial direction ", " circumferential direction " be orientation based on the figure or
Positional relationship is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must
There must be specific orientation, be constructed and operated in a specific orientation, therefore be not considered as limiting the invention.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details.
Claims (7)
1. a kind of efficient nitrogen-cycle bacteria is ecological bed, including efficient nitrogen-cycle bacteria release, ecological floating island and the fixed life
The fixation device on state chinampa, which is characterized in that the ecological floating island includes multiple PVC sticks, and the edge of the PVC stick is equipped with evagination
Buckle and indent locked groove, PVC stick one end center offer round hole, and another end surfaces of the PVC stick are equipped with boss, institute
The regular polygon pipe that boss center penetrates the round hole equipped with one is stated, the regular polygon pipe is located at described boss one end and opens
It puts, other end closing, the efficient nitrogen-cycle bacteria release is fixedly installed on regular polygon pipe open end, the circle
Hole is fixedly connected with the regular polygon pipe by multiple connecting plates, and the connecting plate and the regular polygon pipe are by the circle
Multiple exhalant regions are divided into inside hole, the regular polygon pipe is equipped with multiple limbers, and the water in the regular polygon pipe is logical
It crosses the multiple limbers to flow into the multiple exhalant region, and is flowed out from the other end.
2. a kind of efficient nitrogen-cycle bacteria according to claim 1 is ecological bed, which is characterized in that the evagination buckle and institute
Indent locked groove is stated to be disposed adjacent.
3. a kind of efficient nitrogen-cycle bacteria according to claim 1 is ecological bed, which is characterized in that the evagination buckle and institute
Indent locked groove is stated to be symmetrical arranged.
4. a kind of efficient nitrogen-cycle bacteria according to claim 1 is ecological bed, which is characterized in that the projection shape is positive
Hexagon.
5. a kind of efficient nitrogen-cycle bacteria according to claim 1 is ecological bed, which is characterized in that the projection shape is circle
Shape.
6. a kind of efficient nitrogen-cycle bacteria according to claim 1 is ecological bed, which is characterized in that the fixed device includes
The sliding slot on river both sides is set, across the multiple crossbeams being arranged in above river, the crossbeam moves along the chute, and adjacent two
Suspention is equipped with the ecological floating island on a crossbeam.
7. a kind of efficient nitrogen-cycle bacteria according to claim 6 is ecological bed, which is characterized in that the ecological floating island quadrangle
It is fixedly connected with sunpender, the sunpender is height-adjustable screw rod, and the upper end of the sunpender is provided with idler wheel, the idler wheel
It is arranged on the crossbeam, and along the cross beam movement, the sunpender upper end, which is provided with, pulls crossbeam.
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