CN109601665A - A kind of cynomorium songaricum Pu'er tea and preparation method thereof - Google Patents
A kind of cynomorium songaricum Pu'er tea and preparation method thereof Download PDFInfo
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- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
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Abstract
The present invention provides a kind of cynomorium songaricum Pu'er tea and preparation method thereof, is related to tea making technology field, is prepared from raw materials including the following parts by weight: 1~5 part and 2~50 parts of Yunnan Pu'er cooked tea of Herba Cynomorii extract；Contain cynomorium songaricum polysaccharide and cynomorium songaricum polyphenol in the Herba Cynomorii extract.Refreshed oneself significant effect using cynomorium songaricum Pu'er tea provided by the invention, also there is warm stomach effect, mda content is effectively reduced, improved mouse liver superoxide dismutase activity, can effectively prevent the damage caused by body of free radical and its metabolite.
Description
Technical field
The present invention relates to tea making technology fields, and in particular to a kind of cynomorium songaricum Pu'er tea and preparation method thereof.
Background technique
The earliest function of tea be it is medicinal, Shennong's Herbal record, shennong went into the mountains collecting, tasting and testing different kinds of herbs to be used as medicine, day meet 72 poison, tea and solve it.
Tealeaves has good medical effect, and the Tang Dynasty is to have " tea medicine " word, in " mountain man is clear to supply " that Song Dynasty woods flood is write, also there is " tea, i.e. medicine
" judgement.
China be to the preparation of tea it is diversified, have smoked beans tea, fragrance tea, ginger salt tea etc..9 beginnings of the century, tealeaves at
Divide gradually clear.By the separation and identification of modern science, chemical component is contained in tealeaves up to more than 450 kinds, inorganic mineral is first
Element is up to more than 40 kinds.Chemical component has tea polyphenols, vegetable soda, protein, amino acid, vitamin, pectin element, organic acid, rouge
Polysaccharide, carbohydrate, enzyme, pigment etc..
Pu'er cooked tea, by ripe loose tea made of artificial rapid-result fermentation, there is warm stomach to protect stomach function with big-leaf species in yunnan sundrying tea
Effect, can detoxify, defaecation.Clear people's Zhao Xuemin's supplementary Amplifications of the Compendium of Materia Medica is recorded: " Pu'er tea bitter solves greasy cattle and sheep poison -- include intestines
Remove heat by catharsis ", have the function of helping digestion.Pu'er tea flavour it is warm-natured and, after the mellow Pu'er tea of sweet cunning enters human body stomach, protection can be formed
Film is attached to the surface layer of stomach, and long-term drinking Pu'er tea can play the role of nourishing the stomach, shield stomach.
Contain tea polyphenols, catechin, tea polysaccharide, theophylline, anthocyanidin etc. in Pu'er tea.Tea Polysaccharides compound is commonly referred to as
Tea polysaccharide is the mixture that the element of the first species is complicated and changes greatly, and tea polysaccharide is a kind of acidoglycoprotein, and saccharide portion is by Ah
Draw uncle's sugar, xylose, ribose, glucose and galactolipin to form, and be combined with a large amount of mineral matter element, main calcic, magnesium, iron,
Manganese and a small amount of microelement etc..Catechin is the peculiar composition of tealeaves, is that a kind of important non-enzymatic generated in plant resists
Oxidant is the main active of tea polyphenols, has sterilization, anti-oxidation efficacy, and major function is scavenging capacity oxygen radical,
Anti-lipid peroxidation and the endogenous enzyme activity for adjusting and activating internal oxygen radical.But do not disclose in the prior art as
What can further increase the antioxidant activity of Pu'er tea.
Summary of the invention
The purpose of the present invention is to provide a kind of cynomorium songaricum Pu'er tea and preparation method thereof is provided, using lock provided by the invention
Positive Pu'er tea improves the antioxidant activity of Pu'er tea.
The present invention provides a kind of cynomorium songaricum Pu'er teas, are prepared from raw materials including the following parts by weight: Herba Cynomorii extract 1
~5 parts and 2~50 parts of Yunnan Pu'er cooked tea；
Contain cynomorium songaricum polysaccharide and cynomorium songaricum polyphenol in the Herba Cynomorii extract.
Preferably, the mass percentage of the cynomorium songaricum polysaccharide in the cynomorium songaricum Pu'er tea is 10~15.7%.
Preferably, the mass percentage of the cynomorium songaricum polyphenol in the cynomorium songaricum Pu'er tea is 15~20.3%.
Preferably, the Herba Cynomorii extract preparation method the following steps are included:
1) it is soaked in after being sliced cynomorium songaricum in ethanol solution, 2~10h is extracted at 40~60 DEG C, extracted 3 times, merging mentions
After taking liquid, removing ethyl alcohol, the extracting solution for obtaining the polyphenol containing cynomorium songaricum and the cynomorium songaricum after alcohol extracting；
2) cynomorium songaricum after the alcohol extracting for obtaining the step 1) is dry, obtained drying cynomorium songaricum is soaked in water, 80
1~12h is extracted at~100 DEG C, and obtained extracting solution is filtered, the extracting solution containing cynomorium songaricum polysaccharide is obtained；
3) the mentioning containing cynomorium songaricum polysaccharide for obtaining the extracting solution for the polyphenol containing cynomorium songaricum that the step 1) obtains with step 2)
It takes liquid to mix, obtains cynomorium songaricum extracting solution, the cynomorium songaricum extracting solution is dry, obtain Herba Cynomorii extract.
Preferably, the concentration of the step 1) ethanol solution is 65~95%.
Preferably, the time that the step 1) is impregnated is 2~for 24 hours.
Preferably, the pH value of the step 2) water is 4~8.5.
Preferably, the time that the step 2) is impregnated is 2~for 24 hours.
Preferably, the extracting solution of step 3) polyphenol containing cynomorium songaricum also wraps after mixing with the extracting solution containing cynomorium songaricum polysaccharide
Include: the mixed liquor filter membrane that will be obtained, obtained mistake film liquid are cynomorium songaricum extracting solution；
The aperture of the filter membrane is 0.1~0.22 micron.
The present invention also provides the preparation methods of the cynomorium songaricum Pu'er tea described in above-mentioned technical proposal, comprising: by the cynomorium songaricum
Extract is mixed with Yunnan Pu'er cooked tea, obtains cynomorium songaricum Pu'er tea.
The present invention provides a kind of cynomorium songaricum Pu'er teas, are prepared from raw materials including the following parts by weight: Herba Cynomorii extract 1
~5 parts and 2~50 parts of Yunnan Pu'er cooked tea.In the present invention, the polysaccharide in the Herba Cynomorii extract and polyphenol and Pu'er cooked tea
Common allotment, significant effect of refreshing oneself have warm stomach effect, and mda content is effectively reduced, and improve mouse liver superoxides discrimination
Change enzymatic activity, can effectively prevent the damage caused by collective of free radical and its metabolite.
The embodiment of the present invention is as the result is shown: being refreshed oneself significant effect using cynomorium songaricum Pu'er tea provided by the invention, is had warm
Stomach effect, is effectively reduced mda content, improves mouse liver superoxide dismutase activity, can effectively prevent free radical and its
Metabolite is damaged caused by collective.
Detailed description of the invention
Fig. 1 is the infrared spectroscopy essential groups distribution map of cynomorium songaricum extracting solution.
Specific embodiment
The present invention provides a kind of cynomorium songaricum Pu'er teas, are prepared from raw materials including the following parts by weight: Herba Cynomorii extract 1
~5 parts and 2~50 parts of Yunnan Pu'er cooked tea；Contain cynomorium songaricum polysaccharide and cynomorium songaricum polyphenol in the Herba Cynomorii extract.
In the present invention, the cynomorium songaricum is preferably the cynomorium songaricum of Dulan cultivation.
In the present invention, the mass percentage of the cynomorium songaricum polysaccharide in the cynomorium songaricum Pu'er tea is preferably 10~15.7%.
In the present invention, the mass percentage of the cynomorium songaricum polyphenol in the cynomorium songaricum Pu'er tea is preferably 15~20.3%.
It is 1~5 part of Herba Cynomorii extract that cynomorium songaricum Pu'er tea provided by the invention, which includes parts by weight, preferably 2~4 parts.
In the present invention, cynomorium songaricum polysaccharide and cynomorium songaricum polyphenol are contained in the Herba Cynomorii extract.
In the present invention, the preparation method of the Herba Cynomorii extract preferably includes following steps:
1) it is soaked in after being sliced cynomorium songaricum in ethanol solution, 2~10h is extracted at 40~60 DEG C, extracted 3 times, merging mentions
After taking liquid, removing ethyl alcohol, the extracting solution for obtaining the polyphenol containing cynomorium songaricum and the cynomorium songaricum after alcohol extracting；
2) cynomorium songaricum after the alcohol extracting for obtaining the step 1) is dry, obtained drying cynomorium songaricum is soaked in water, 80
1~12h is extracted at~100 DEG C, and obtained extracting solution is filtered, the extracting solution containing cynomorium songaricum polysaccharide is obtained；
3) the mentioning containing cynomorium songaricum polysaccharide for obtaining the extracting solution for the polyphenol containing cynomorium songaricum that the step 1) obtains with step 2)
It takes liquid to mix, obtains cynomorium songaricum extracting solution, the cynomorium songaricum extracting solution is dry, obtain Herba Cynomorii extract.
The present invention is soaked in ethanol solution after being sliced cynomorium songaricum, and 2~10h is extracted at 40~60 DEG C, is extracted 3 times, is closed
And after extracting solution, removing ethyl alcohol, the extracting solution for obtaining the polyphenol containing cynomorium songaricum and the cynomorium songaricum after alcohol extracting.
The slice thickness and be not particularly limited using slice tool that the present invention is sliced the cynomorium songaricum be using conventional
Can, the slice tool is preferably the wooden knife.
In the present invention, the concentration of the ethanol solution is preferably 65~95%, and more preferably 70~90%, most preferably
75~85%.In the present invention, the time that the cynomorium songaricum after the slice is impregnated in ethanol solution is preferably 2~for 24 hours, more preferably
For 5~20h, most preferably 10~15h.
In the present invention, the temperature of the extraction is preferably 40~60 DEG C, and more preferably 50 DEG C；The time of the extraction is excellent
It is selected as 2~10h, more preferably 4~8h, most preferably 5~6h；The number of the extraction is preferably 3 times.
Cynomorium songaricum after the alcohol extracting that the present invention obtains is dry, obtained drying cynomorium songaricum is soaked in water, at 80~100 DEG C
1~12h is extracted, obtained extracting solution is filtered, the extracting solution containing cynomorium songaricum polysaccharide is obtained.
In the present invention, the dry cynomorium songaricum be soaked in the time in water be preferably 2~for 24 hours, more preferably 5~20h, most
Preferably 10~15h.In the present invention, the time of the extraction is preferably 1~12h, more preferably 4~10h, and most preferably 5
~8h；The temperature of the extraction is preferably 80~100 DEG C.In the present invention, the pH value of the water is preferably 4~8.5, more preferably
It is 5.8~7.0.
The present invention mixes the extracting solution of the obtained polyphenol containing cynomorium songaricum with the obtained extracting solution containing cynomorium songaricum polysaccharide, obtains
Cynomorium songaricum extracting solution, the cynomorium songaricum extracting solution is dry, obtain Herba Cynomorii extract.
In the present invention, the extracting solution of the polyphenol containing cynomorium songaricum preferably also wraps after mixing with the extracting solution containing cynomorium songaricum polysaccharide
Include: the mixed liquor filter membrane that will be obtained, obtained mistake film liquid are cynomorium songaricum extracting solution.In the present invention, the aperture of the filter membrane is
0.1~0.22 micron.In the present invention, the temperature when mixed liquor filter membrane is preferably 30~50 DEG C, more preferably 35~
45 DEG C, most preferably 40 DEG C.
It is 2~50 parts of Yunnan Pu'er cooked tea that cynomorium songaricum Pu'er tea provided by the invention, which includes parts by weight, preferably 10~
40 parts, more preferably 15~25 parts.The present invention is not particularly limited the source of the Yunnan Pu'er cooked tea, using conventional commercial
Product.
The present invention also provides the preparation methods of the cynomorium songaricum Pu'er tea described in above-mentioned technical proposal, comprising: by the cynomorium songaricum
Extract is mixed with Yunnan Pu'er cooked tea, obtains cynomorium songaricum Pu'er tea.
A kind of cynomorium songaricum Pu'er tea of the present invention and preparation method thereof is done further in detail combined with specific embodiments below
Thin introduction, technical solution of the present invention include but is not limited to following embodiment.
Embodiment 1
1) cynomorium songaricum that fresh Dulan is cultivated is cleaned up, the wooden knife slice, 65% ethyl alcohol impregnates 2 hours, 40
10h is extracted at DEG C, is extracted 3 times, after combined extract, removing ethyl alcohol, the extracting solution and the lock after alcohol extracting that obtain the polyphenol containing cynomorium songaricum
Sun.
2) cynomorium songaricum after alcohol extracting is dry, it is 6.8 that water, which is added, and adjusts pH value, impregnates 2 hours, it is small that 2.5 are extracted at 80 DEG C
When, obtained extracting solution is filtered, extracting solution containing cynomorium songaricum polysaccharide is obtained.
3) extracting solution containing cynomorium songaricum polysaccharide obtained by the above-mentioned extracting solution of polyphenol containing cynomorium songaricum 1) obtained and 2) merges, at 30 DEG C
Cross film, membrane aperture is 0.22 micron, obtains cynomorium songaricum extracting solution, measure content, polyphenol containing cynomorium songaricum 15.8%, contain cynomorium songaricum polysaccharide in
12.5%.Spray drying, obtains Herba Cynomorii extract.
4) 2 parts of Yunnan Pu'er cooked tea are weighed, 1 part of above-mentioned Herba Cynomorii extract is added, compression moulding obtains cynomorium songaricum Pu'er tea.
Embodiment 2
1) cynomorium songaricum that fresh Dulan is cultivated is cleaned up, the wooden knife slice, 95% ethyl alcohol impregnates 24 hours, 60
2h is extracted at DEG C, is extracted 3 times, after combined extract, removing ethyl alcohol, the extracting solution and the lock after alcohol extracting that obtain the polyphenol containing cynomorium songaricum
Sun.
2) cynomorium songaricum after alcohol extracting is dry, it is 5.5 that water, which is added, and adjusts pH value, impregnates 24 hours, it is small that 12 are extracted at 100 DEG C
When, obtained extracting solution is filtered, extracting solution containing cynomorium songaricum polysaccharide is obtained.
3) extracting solution containing cynomorium songaricum polysaccharide obtained by the above-mentioned extracting solution of polyphenol containing cynomorium songaricum 1) obtained and 2) merges, at 50 DEG C
Film is crossed, membrane aperture is 0.1 micron, obtains cynomorium songaricum extracting solution, measures content, and polyphenol containing cynomorium songaricum 20.2% contains cynomorium songaricum polysaccharide
14.9%.Spray drying, obtains Herba Cynomorii extract.
4) 50 parts of Yunnan Pu'er cooked tea are weighed, 5 parts of above-mentioned Herba Cynomorii extract is added, compression moulding obtains cynomorium songaricum Pu'er tea.
Embodiment 3
1) cynomorium songaricum that fresh Dulan is cultivated is cleaned up, the wooden knife slice, 85% ethyl alcohol impregnates 12 hours, 50
8h is extracted at DEG C, is extracted 3 times, after combined extract, removing ethyl alcohol, the extracting solution and the lock after alcohol extracting that obtain the polyphenol containing cynomorium songaricum
Sun.
2) cynomorium songaricum after alcohol extracting is dry, it is 4.0 that water, which is added, and adjusts pH value, impregnates 16 hours, it is small that 8 are extracted at 90 DEG C
When, obtained extracting solution is filtered, extracting solution containing cynomorium songaricum polysaccharide is obtained.
3) extracting solution of polyphenol containing cynomorium songaricum obtained by the above-mentioned extracting solution of polyphenol containing cynomorium songaricum 1) obtained and 2) merges, at 40 DEG C
Film is crossed, membrane aperture is 0.22 micron, obtains cynomorium songaricum extracting solution, measures content, and polyphenol containing cynomorium songaricum 18.7% contains cynomorium songaricum polysaccharide
15.0%.Spray drying, obtains Herba Cynomorii extract.
4) 25 parts of Yunnan Pu'er cooked tea are weighed, 2 parts of above-mentioned Herba Cynomorii extract is added, compression moulding obtains cynomorium songaricum Pu'er tea.
Embodiment 4
1) cynomorium songaricum that fresh Dulan is cultivated is cleaned up, the wooden knife slice, 75% ethyl alcohol impregnates 6 hours, 50
DEG C choosing extract 9h, extract 3 times, combined extract, remove ethyl alcohol after, the extracting solution and the lock after alcohol extracting that obtain the polyphenol containing cynomorium songaricum
Sun.
2) cynomorium songaricum after alcohol extracting is dry, it is 5.8 that water, which is added, and adjusts pH value, impregnates 8 hours, it is small that 12 are extracted at 85 DEG C
When, obtained extracting solution is filtered, extracting solution containing cynomorium songaricum polysaccharide is obtained.
3) extracting solution containing cynomorium songaricum polysaccharide obtained by the above-mentioned extracting solution of polyphenol containing cynomorium songaricum 1) obtained and 2) merges, at 45 DEG C
Film is crossed, membrane aperture is 0.1 micron, obtains cynomorium songaricum extracting solution, measures content, and polyphenol containing cynomorium songaricum 19.6% contains cynomorium songaricum polysaccharide
13.4%.Spray drying, obtains Herba Cynomorii extract.
4) 50 parts of Yunnan Pu'er cooked tea are weighed, 1 part of above-mentioned Herba Cynomorii extract is added, compression moulding obtains cynomorium songaricum Pu'er tea.
Embodiment 5
Cynomorium songaricum extracting solution prepared by embodiment 1 carries out infrared light detection, the result is shown in Figure 1.
As can be drawn from Figure 1, there is C-O stretching vibration at 1045, compound is polysaccharide；There is phenyl ring skeleton at 1604
Flexible amplitude or NH2 scissors bending vibration, compound are phenols.
Cynomorium songaricum extracting solution prepared by embodiment 1 carries out content detection, the results are shown in Table 1.
1 cynomorium songaricum film of table filters content detection
| Cynomorium songaricum extracting solution | Cynomorium songaricum polyphenol | Cynomorium songaricum polysaccharide |
| Content | 15.8% | 12.5% |
Embodiment 6
By healthy KM mouse 40,4 groups are randomly divided into, half male and half female gives prepared by embodiment 1 low dose of cynomorium songaricum Pu'er tea
Amount group and high dose group (5g/kg/d, 10g/kg/d), blank group press 0.2ml/10g stomach-filling pure water, give cynomorium songaricum Pu'er tea 30
It.The content for detecting malonaldehyde (MDA), superoxide dismutase (SOD), the results are shown in Table 2.
The influence of 2 cynomorium songaricum Pu'er tea mouse liver biochemical indicator of table
Compared with blank group: P < 0.05 *, P < 0.01 * *
By table 2, it can be concluded that, cynomorium songaricum Pu'er tea can improve mouse liver superoxide dismutase activity, reduce malonaldehyde and contain
Amount, can effectively prevent the damage caused by collective of free radical and its metabolite.
By healthy KM mouse 30,3 groups are randomly divided into, cynomorium songaricum Pu'er tea two of half male and half female, the preparation of embodiment 1 are administered
Group (low, high dose group dosage is respectively 5g/kg/d, 10g/kg/d), blank control group are administered seven days.Detect gastric juice amount, stomach
The content of sour (pH value), the results are shown in Table 3.
Influence after 3 cynomorium songaricum Pu'er tea stomach-filling of table 30 days to gastric juice index
| Group | Dosage (g/kg) | Gastric juice amount (ml) | Gastric acid (pH value) |
| Low dose group | 5 | 3.12±0.48* | 2.08±0.41** |
| High dose group | 10 | 3.24±0.26** | 2.21±0.32** |
| Blank group | —— | 2.35±0.30 | 3.54±0.50 |
| Model group | —— | 3.77±0.54## | 1.86±0.29## |
Compared with blank group: #P < 0.05, ##P < 0.01；Compared with model group: P < 0.05 *, P < 0.01 * *
By table 3, it can be concluded that, compared with blank group, model group gastric juice amount obviously increases (P < 0.01).Compared with model group,
Two dosage group gastric juice amounts of cynomorium songaricum Pu'er tea significantly reduce.Compared with blank group, model group gastric acid (pH value) be substantially reduced (P <
0.01).Compared with model group, two dosage group gastric acids (pH value) of cynomorium songaricum Pu'er tea are significantly raised.
It can be concluded that, refreshed oneself significant effect using cynomorium songaricum Pu'er tea provided by the invention by above embodiments, there is warm stomach
Mda content is effectively reduced in effect, improves mouse liver superoxide dismutase activity, can effectively prevent free radical and its generation
Thank to product damage caused by collective.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of cynomorium songaricum Pu'er tea, which is characterized in that be prepared from raw materials including the following parts by weight: Herba Cynomorii extract 1~5
Part and 2~50 parts of Yunnan Pu'er cooked tea；
Contain cynomorium songaricum polysaccharide and cynomorium songaricum polyphenol in the Herba Cynomorii extract.
2. cynomorium songaricum Pu'er tea according to claim 1, which is characterized in that the matter of the cynomorium songaricum polysaccharide in the cynomorium songaricum Pu'er tea
Measuring percentage composition is 10~15.7%.
3. cynomorium songaricum Pu'er tea according to claim 1, which is characterized in that the matter of the cynomorium songaricum polyphenol in the cynomorium songaricum Pu'er tea
Measuring percentage composition is 15~20.3%.
4. cynomorium songaricum Pu'er tea according to claim 1, which is characterized in that the preparation method of the Herba Cynomorii extract include with
Lower step:
1) it is soaked in after being sliced cynomorium songaricum in ethanol solution, 2~10h is extracted at 40~60 DEG C, extracted 3 times, combined extract,
After removing ethyl alcohol, the extracting solution for obtaining the polyphenol containing cynomorium songaricum and the cynomorium songaricum after alcohol extracting；
2) cynomorium songaricum after the alcohol extracting for obtaining the step 1) is dry, obtained drying cynomorium songaricum is soaked in water, 80~100
1~12h is extracted at DEG C, and obtained extracting solution is filtered, the extracting solution containing cynomorium songaricum polysaccharide is obtained；
3) extracting solution containing cynomorium songaricum polysaccharide for obtaining the extracting solution for the polyphenol containing cynomorium songaricum that the step 1) obtains and step 2)
Mixing, obtains cynomorium songaricum extracting solution, and the cynomorium songaricum extracting solution is dry, obtains Herba Cynomorii extract.
5. cynomorium songaricum Pu'er tea according to claim 4, which is characterized in that the concentration of the step 1) ethanol solution be 65~
95%.
6. cynomorium songaricum Pu'er tea according to claim 4, which is characterized in that the time that the step 1) is impregnated is 2~for 24 hours.
7. cynomorium songaricum Pu'er tea according to claim 4, which is characterized in that the pH value of the step 2) water is 4~8.5.
8. cynomorium songaricum Pu'er tea according to claim 4, which is characterized in that the time that the step 2) is impregnated is 2~for 24 hours.
9. cynomorium songaricum Pu'er tea according to claim 4, which is characterized in that the extracting solution of step 3) polyphenol containing cynomorium songaricum with
After extracting solution mixing containing cynomorium songaricum polysaccharide further include: the mixed liquor filter membrane that will be obtained, obtained mistake film liquid are cynomorium songaricum extraction
Liquid；
The aperture of the filter membrane is 0.1~0.22 micron.
10. the preparation method of cynomorium songaricum Pu'er tea according to any one of claims 1 to 9, comprising: by the Herba Cynomorii extract and cloud
Southern Pu'er cooked tea mixing, obtains cynomorium songaricum Pu'er tea.
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