CN109576321A - A kind of agriculture astaxanthin and preparation method thereof - Google Patents
A kind of agriculture astaxanthin and preparation method thereof Download PDFInfo
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- CN109576321A CN109576321A CN201811625576.1A CN201811625576A CN109576321A CN 109576321 A CN109576321 A CN 109576321A CN 201811625576 A CN201811625576 A CN 201811625576A CN 109576321 A CN109576321 A CN 109576321A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
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Abstract
The present invention relates to a kind of agriculture astaxanthins and preparation method thereof, are made of astaxanthin, chitin and chitin, it is characterised in that also contain canthaxanthin.Agricultural astaxanthin is obtained by crab shell and/or shrimp shell through microbial fermentation, and the microorganism is two kinds of one of lactic acid bacteria and saccharomycete or arbitrary proportion.Astaxanthin prepared by the present invention is applied to agricultural, and the coloring and storage stability of fruit can be improved.
Description
Technical field
The present invention relates to a kind of agriculture astaxanthins and preparation method thereof.
Background technique
Natural astaxanthin is primarily present in certain animals, algae and microbial body, production can be divided into from animal and its
It is extracted in byproduct, microbial fermentation is extracted and used from algae.It is extracted from animal and byproduct, astaxanthin is largely distributed
In in the internal and molluscan shell of aquatic animal (good-win, 1984), these animals itself are unable to synthesizing astaxanthin, it
Intracorporal astaxanthin all derive from food (1932) algae mainly in water, karrer etc. extracted from crab ovum earliest
After astaxanthin, the by-product of Crustachia aquatic products (shrimp, crab) is always the primary raw material for extracting natural astaxanthin.In Norway, adopt
After crushing shrimp shell, with acid or enzymatic hydrolysis, astaxanthin finally is extracted with the method that organic solvent extracts.Such method mentions
Take rate low, and there are waste water and exhaust gas, pollute environment.
The method for extracting agriculture astaxanthin in shrimp and crab shells using microorganism at present, majority have ignored the extraction of canthaxanthin,
And canthaxanthin has raising crop immunity applied to agriculture, promotes fruit color, reduction fertilizer amount and raising fruit are hard
The effect of degree and resistant storage properties.
In addition, being extracted using microorganism, extraction time is longer, at high cost.
Summary of the invention
The present invention provides a kind of agriculture astaxanthin and preparation method thereof, and solving technical problem is the bacterium that 1) selection is suitable
Strain, extracts shrimp and crab shells, shortens extraction time；2) increase the content of canthaxanthin in astaxanthin extract.
In order to solve the above-mentioned technical problem, the invention adopts the following technical scheme:
A kind of agriculture astaxanthin, is made of astaxanthin, chitin and chitin, also contains canthaxanthin.
Chitin, chitin, astaxanthin and canthaxanthin mass ratio be 56~85:0.8~5:3~10:0.8~5.
Preferably, the agriculture astaxanthin is obtained by crab shell and/or shrimp shell through microbial fermentation；
The microorganism is two kinds of one of acid-producing bacteria and saccharomycete or arbitrary proportion.
Preferably, the microorganism is the composition of acid-producing bacteria and saccharomycete.
Preferably, the acid-producing bacteria is clostridium；The saccharomycete is shadow yeast.
Preferably, the clostruidium is clostridium butyricum；The shadow yeast buying is in Chinese industrial microbial bacteria
Kind preservation administrative center deposit number is CICC 33080.
A kind of preparation method of agriculture astaxanthin, follows the steps below:
1) it is crushed and impregnates: being added to after crab shell and/or shrimp shell are crushed in the solution that pH is 1.5~3.2, impregnate 20~60min,
Obtain soak；
2) filtering cleaning: soak is cleaned after filtering, and cleaning to pH is 6.0~7.2, obtains cleaning materials；
3) it preparing agriculture astaxanthin: cleaning materials is added in fermentor, tune water content to 60%~75% accesses microorganism,
Anaerobic fermentation is carried out to get agricultural astaxanthin.
Fermentation condition described in step 3) is that oxygen content is 0.5mg~5mg；Fermentation time is 5~7 days；Cleaning materials and micro-
The mass ratio of biology is 99.8~99.999:0.001~0.2.
Fermentation condition described in step 3) is that temperature is 15~25 DEG C.
Invention has following advantageous effects:
1. canthaxanthin content in astaxanthin can be improved in the application, being applied to agricultural has raising crop immunity, promotes fruit
Coloring reduces fertilizer amount and improves the effect of the hardness of fruit and resistant storage properties.
2. the application can shorten fermentation time using acid-producing bacteria and saccharomycete combined ferment.
Specific embodiment
The present invention is further illustrated below with reference to specific example.
Embodiment 1
A kind of agriculture astaxanthin, is made of astaxanthin, chitin and chitin, also contains canthaxanthin.
Chitin content is 67.8%, chitin content is 2.3%, content astaxanthin is 7.4% and canthaxanthin content is
3.5%。
Embodiment 2
A kind of agriculture astaxanthin, is made of astaxanthin, chitin and chitin, also contains canthaxanthin.
Chitin content is 67.8%, chitin content is 2.3%, content astaxanthin is 7.4% and canthaxanthin content is
3.5%。
A kind of preparation method of agriculture astaxanthin, follows the steps below:
1) it is crushed and impregnates: shrimp shell being added in the solution that pH is 1.5~2.2 after broken, impregnate 20~30min, obtain soak；
2) filtering cleaning: soak is cleaned after filtering, and cleaning to pH is 6.5~7.2, obtains cleaning materials；
3) it prepares agriculture astaxanthin: cleaning materials is added in fermentor, the mass ratio of cleaning materials and microorganism is 99.998:
0.002；Adjust water content to 65%~70%, access microorganism, carry out anaerobic fermentation, fermentation condition be oxygen content be 0.8mg~
1.2mg, temperature are 18~20 DEG C, ferment 7 days to get agricultural astaxanthin.
The microorganism is the composition of clostridium butyricum and shadow yeast according to mass ratio 1:8；The shadow yeast buying
In Chinese industrial Microbiological Culture Collection administrative center deposit number be CICC 33080.
Embodiment 3
A kind of agriculture astaxanthin, is made of astaxanthin, chitin and chitin, also contains canthaxanthin.
Chitin content is 68.4%, chitin content is 2.6%, content astaxanthin 2.6%, canthaxanthin 0.2.
A kind of preparation method of agriculture astaxanthin, follows the steps below:
1) it is crushed and impregnates: shrimp shell being added in the solution that pH is 1.5~2.2 after broken, impregnate 20~30min, obtain soak；
2) filtering cleaning: soak is cleaned after filtering, and cleaning to pH is 6.5~7.2, obtains cleaning materials；
3) it prepares agriculture astaxanthin: cleaning materials is added in fermentor, the mass ratio of cleaning materials and microorganism is 99.998:
0.002；Adjust water content to 65%~70%, access microorganism, carry out anaerobic fermentation, fermentation condition be oxygen content be 0.8mg~
1.2mg, temperature are 26~28 DEG C, ferment 7 days to get agricultural astaxanthin.
The microorganism is the composition of clostridium butyricum and shadow yeast according to mass ratio 1:8；The shadow yeast buying
In Chinese industrial Microbiological Culture Collection administrative center deposit number be CICC 33080.
Embodiment 4
A kind of agriculture astaxanthin, is made of astaxanthin, chitin and chitin, also contains canthaxanthin.
Chitin content is 68.2%, chitin content is 2.4%, content astaxanthin 1.8%, canthaxanthin 0.1.
A kind of preparation method of agriculture astaxanthin, follows the steps below:
1) it is crushed and impregnates: being added to after crab shell and/or shrimp shell are crushed in the solution that pH is 1.5~2.2, impregnate 20~30min,
Obtain soak；
2) filtering cleaning: soak is cleaned after filtering, and cleaning to pH is 6.5~7.2, obtains cleaning materials；
3) it prepares agriculture astaxanthin: cleaning materials is added in fermentor, the mass ratio of cleaning materials and microorganism is 99.998:
0.002；Adjust water content to 65%~70%, access microorganism, carry out aerobic fermentation, fermentation condition be oxygen content be 16mg~
18mg, temperature are 28~30 DEG C, ferment 7 days to get agricultural astaxanthin.
The microorganism is the composition of clostridium butyricum and shadow yeast according to mass ratio 1:8；The shadow yeast buying
In Chinese industrial Microbiological Culture Collection administrative center deposit number be CICC 33080.
Embodiment 5
A kind of agriculture astaxanthin, is made of astaxanthin, chitin and chitin, also contains canthaxanthin.
Chitin content is 68.5%, chitin content is 2.5%, content astaxanthin 10.2%, canthaxanthin 0.3.
A kind of preparation method of agriculture astaxanthin, follows the steps below:
1) it is crushed and impregnates: shrimp shell being added in the solution that pH is 1.5~2.2 after broken, impregnate 20~30min, obtain soak；
2) filtering cleaning: soak is cleaned after filtering, and cleaning to pH is 6.5~7.2, obtains cleaning materials；
3) it prepares agriculture astaxanthin: cleaning materials is added in fermentor, the mass ratio of cleaning materials and microorganism is 99.998:
0.002；Adjust water content to 65%~70%, access microorganism, carry out anaerobic fermentation, fermentation condition be oxygen content be 16mg~
18mg, temperature are 18~20 DEG C, ferment 7 days to get agricultural astaxanthin.
The microorganism is the composition of clostridium butyricum and shadow yeast according to mass ratio 1:8；The shadow yeast buying
In Chinese industrial Microbiological Culture Collection administrative center deposit number be CICC 33080.
Embodiment 6
A kind of agriculture astaxanthin, is made of astaxanthin, chitin and chitin, also contains canthaxanthin.
A kind of preparation method of agriculture astaxanthin, follows the steps below:
1) it is crushed and impregnates: will climb after shrimp shell is crushed and be added in the solution that pH is 3.0, and impregnate 20~30min, obtain soak；
2) filtering cleaning: soak is cleaned after filtering, and cleaning to pH is 6.2~6.8, obtains cleaning materials；
3) it prepares agriculture astaxanthin: cleaning materials is added in fermentor, the mass ratio of cleaning materials and microorganism is 99.9:
0.01；It adjusts water content to 70%~75%, accesses microorganism, carry out anaerobic fermentation, fermentation condition is that oxygen content is 1.5mg~2mg,
Temperature is 20~22 DEG C, ferments 5 days to get agricultural astaxanthin.
The microorganism is the composition of clostridium butyricum and shadow yeast according to mass ratio 1:3.
Embodiment 7
A kind of agriculture astaxanthin, is made of astaxanthin, chitin and chitin, also contains canthaxanthin.
A kind of preparation method of agriculture astaxanthin, follows the steps below:
1) it is crushed and impregnates: being added to after shrimp shell and crab shell mixture are crushed in the solution that pH is 2.4, impregnate 45~50min, obtain
Soak；
2) filtering cleaning: soak is cleaned after filtering, and cleaning to pH is 7.0~7.2, obtains cleaning materials；
3) it prepares agriculture astaxanthin: cleaning materials is added in fermentor, the mass ratio of cleaning materials and microorganism is 99.995:
0.005；Adjust water content to 70%~75%, access microorganism, carry out anaerobic fermentation, fermentation condition be oxygen content be 3.2mg~
4mg, temperature are 22~24 DEG C, ferment 6 days to get agricultural astaxanthin.
The microorganism is the composition of clostridium butyricum and shadow yeast according to mass ratio 3:2.
Embodiment 8
A kind of agriculture astaxanthin, is made of astaxanthin, chitin and chitin, also contains canthaxanthin.
A kind of preparation method of agriculture astaxanthin, follows the steps below:
1) it is crushed and impregnates: crab shell being added in the solution that pH is 2.5 after broken, impregnate 55~60min, obtain soak；
2) filtering cleaning: soak is cleaned after filtering, and cleaning to pH is 7.0~7.2, obtains cleaning materials；
3) it prepares agriculture astaxanthin: cleaning materials is added in fermentor, the mass ratio of cleaning materials and microorganism is 99.95:
0.05；It adjusts water content to 70%~75%, accesses microorganism, carry out anaerobic fermentation, fermentation condition is that oxygen content is 3.2mg~4mg,
Temperature is 22~24 DEG C, ferments 6 days to get agricultural astaxanthin.
The microorganism is the composition of clostridium and saccharomycete according to mass ratio 3:2.
Beneficial effects of the present invention are further illustrated below with reference to experimental data:
It is fermented, is made using clostridium butyricum and shadow yeast prawn shell it can be seen from the data of embodiment 2 to embodiment 5
Standby agriculture astaxanthin, the content of astaxanthin and canthaxanthin is related with fermentation condition in the product of preparation, using anaerobic fermentation,
Fermentation temperature is 15~25 DEG C than conveniently, temperature is higher than astaxanthin and canthaxanthin content in 26 DEG C of embodiment 3 and embodiment 4
Seldom.
Experiment one
Material to be tested
1 materials and methods:
1.1 test sites: Gansu Agriculture University laboratory.
1.2 experiment detections: taking 20 apples from each hillslope processes at random, detects the transverse diameter of apple, color, hardness, solvable
Property solid content and total acid content.
1.3 materials to be tested: by blank (substance in addition to fertilizer is not added in 19-19-19 Water soluble fertilizer), embodiment 2, reality
It applies example 3, embodiment 4 and embodiment 5 to be added in the Water soluble fertilizer of 19-19-19 according to the mass ratio of 4:96 respectively, with the water of preparation
Molten fertilizer is experimental material.
1.4 experimental methods:
1 mu of apple orchard is divided into 6 cells, addition blank (is not added 100 square metres of every cell in 19-19-19 Water soluble fertilizer
Substance in addition to fertilizer), comparison 1(is except without in addition to canthaxanthin, other materials content and embodiment 4 are consistent), embodiment 2,
The Water soluble fertilizer of embodiment 3, embodiment 4 and embodiment 5 is after Apple Bags, by way of sprinkling irrigation, applies and arrives respective experiment
In cell, every each dose of cell is 10kg, and extension rate 800 is primary at interval of fertilising in 20 days, is stopped until plucking 15 days before bag
Only apply fertilizer.
In addition to experiment process is different, other operations are consistent for this experiment；Apple tree kind is Qin Fu 1, the age of tree 12 years.
2. testing result
Average transverse diameter, color, hardness, solid acid ratio, soluble solid and the total acid content of apple are shown in Table 1
By above-mentioned experiment compare it can be seen from the application can significantly improve the quality of apple, while being influenced by tunning,
There is also differences for the final result of the application improvement crop quality, wherein to add anaerobism, tunning under lower temperature conditions
The effect of embodiment 2 is best, at the same by embodiment 5 and 2 data of embodiment compare it can be seen between astaxanthin and canthaxanthin
There is synergistic effect in terms of improving the quality of crop, effect is more preferable.
Claims (9)
1. a kind of agriculture astaxanthin, is made of, it is characterised in that also contain canthaxanthin astaxanthin, chitin and chitin.
2. agriculture astaxanthin as described in claim 1, which is characterized in that the matter of chitin, chitin, astaxanthin and canthaxanthin
Amount is than being 56~85:0.8~5:3~10:0.8~5.
3. agriculture astaxanthin as claimed in claim 1 or 2, which is characterized in that the agriculture astaxanthin is by crab shell and/or shrimp
Shell is obtained through microbial fermentation；
The microorganism is two kinds of one of acid-producing bacteria and saccharomycete or arbitrary proportion.
4. agriculture astaxanthin as claimed in claim 3, which is characterized in that the microorganism is the combination of acid-producing bacteria and saccharomycete
Object.
5. agriculture astaxanthin as claimed in claim 4, which is characterized in that the acid-producing bacteria is clostridium；The ferment
Female bacterium is shadow yeast.
6. agriculture astaxanthin as claimed in claim 5, which is characterized in that the clostruidium is clostridium butyricum；It is described
Shadow yeast buying is CICC 33080 in Chinese industrial Microbiological Culture Collection administrative center deposit number.
7. a kind of preparation method of agriculture astaxanthin, which is characterized in that follow the steps below:
1) it is crushed and impregnates: being added to after crab shell and/or shrimp shell are crushed in the solution that pH is 1.5~3.2, impregnate 20~60min,
Obtain soak；
2) filtering cleaning: soak is cleaned after filtering, and cleaning to pH is 6.0~7.2, obtains cleaning materials；
3) it preparing agriculture astaxanthin: cleaning materials is added in fermentor, tune water content to 60%~75% accesses microorganism,
Anaerobic fermentation is carried out to get agricultural astaxanthin.
8. the preparation method of agriculture astaxanthin as claimed in claim 7, which is characterized in that fermentation condition described in step 3) is
Oxygen content is 0.5mg~5mg；Fermentation time is 5~7 days；The mass ratio of cleaning materials and microorganism is 99.8~99.999:
0.001~0.2.
9. the preparation method of agriculture astaxanthin as claimed in claim 7, which is characterized in that fermentation condition described in step 3) is
Temperature is 15~25 DEG C.
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Citations (5)
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|---|---|---|---|---|
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| CN101926409A (en) * | 2008-11-28 | 2010-12-29 | 薛晓宁 | Feed additive by mixedly fermenting shrimp heads and vegetable feed proteins and processing method thereof |
| CN103027187A (en) * | 2013-01-05 | 2013-04-10 | 北京嘉博文生物科技有限公司 | Anti-stress fermentation protein feed and producing method thereof |
| CN105503383A (en) * | 2015-12-14 | 2016-04-20 | 潍坊大救星生物科技有限公司 | Application of astaxanthin to water-soluble fertilizer |
-
2018
- 2018-12-28 CN CN201811625576.1A patent/CN109576321A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009032987A1 (en) * | 2007-09-05 | 2009-03-12 | Microbia, Inc. | Isolation of pellet-forming microorganisms |
| CN101215595A (en) * | 2007-12-26 | 2008-07-09 | 大连工业大学 | Method for extracting astaxanthin, protein and chitin from shrimp shell by utilizing microorganism |
| CN101926409A (en) * | 2008-11-28 | 2010-12-29 | 薛晓宁 | Feed additive by mixedly fermenting shrimp heads and vegetable feed proteins and processing method thereof |
| CN103027187A (en) * | 2013-01-05 | 2013-04-10 | 北京嘉博文生物科技有限公司 | Anti-stress fermentation protein feed and producing method thereof |
| CN105503383A (en) * | 2015-12-14 | 2016-04-20 | 潍坊大救星生物科技有限公司 | Application of astaxanthin to water-soluble fertilizer |
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