CN101491290A - Method for producing livestock birds health cultivation green feed additive using distilled grain - Google Patents

Method for producing livestock birds health cultivation green feed additive using distilled grain Download PDF

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Publication number
CN101491290A
CN101491290A CNA2008100690834A CN200810069083A CN101491290A CN 101491290 A CN101491290 A CN 101491290A CN A2008100690834 A CNA2008100690834 A CN A2008100690834A CN 200810069083 A CN200810069083 A CN 200810069083A CN 101491290 A CN101491290 A CN 101491290A
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vinasse
potale
temperature
culture
percent
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CN101491290B (en
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康冀川
文庭池
雷帮星
何劲
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Guizhou University
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Guizhou University
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses a method for producing a green feed additive by potale for beast and bird healthy cultivation. The method comprises the steps of potale mixing subpackage, medium sterilization, liquid strain making, inoculation fermentation, and drying and crushing. The method adopts chlorine dioxide to sterilize a potale culture medium, and Paecilomyces varioti fungus to ferment the potale. In the additive produced by the method, protein content is improved to 26.8 to 30.5 percent from 17 percent, energy value is improved to 4,250kCal/kg from the previous 2,510kCal/kg, and direct available digestibility is improved to 62 percent from 33 percent. Main compositions contain more than 10 kinds of nucleotide substances, mannite, fungal polysaccharide, superoxide dismutase, amino acid, sterol, alkaloid, VE, VB1, VB2, mineral substances and the like. Animal experiments prove that the additive has wide action and efficacy. The method has the characteristics of wide raw material source, easy production, controllable quality, low cost, wide application and the like, is applicable to large-scale industrial production, and provides a new approach for high-value utilization of wine industrial residue.

Description

Utilize vinasse to produce the method for livestock birds health cultivation green feed additive
Technical field
The present invention relates to the fungi fermentation engineering, in particular to utilizing vinasse fermentation to produce the method for livestock birds health cultivation green feed additive.
Background technology
Vinasse are accessory substances of wine-making industry, and amount is concentrated greatly.According to statistics, China only distillers ' grains produce per year and just to reach more than 3,000 ten thousand tons.The content of crude protein reaches about 17% in the distillers ' grains dry, contains rich in amino acid, vitamin and various trace elements etc., and nutrition is abundanter.But their acidity is higher, and is easily corrupt rotten, if untimely processing, inevitable serious environment pollution.So characteristics according to distillers ' grains, people have carried out many-sided research to its resource problem, for example utilize distillers ' grains to produce glycerine, cultivate edible mushroom, extract amino acid and trace element, extraction phytic acid and phytic acid calcium, wine vinegar etc., but maximum still the playing an important role in livestock breeding industry as feed of distillers ' grains.
Utilizing the Production by Microorganism Fermentation single-cell protein feed is the microbe inoculation bacterial classification, makes it be fermented into the feed of easily digesting and assimilating, and this is the developing direction of modern feed industry; But present liquor groove is inoculation yeast bacterium or some bacterium only, and active ingredient is single relatively, and the while, present stage was difficult to large-scale popularization because the condition difficulty is controlled, and complex process is invested height.
In recent years, people pay close attention to the technology of vinasse recycling gradually.The patent application that relates to vinasse fermentation production feed has No. 200510044747.8 " a kind of brewex's grains are produced the method for rich zymoprotein feed ", No. 200810044216.2 " it is the method for main feedstuff that mixed culture fermentation is handled with vinasse ", No. 200610153245.3 " a kind of methods of utilizing paper mulberry leaf and vinasse mixed culture fermentation to prepare high protein feed " etc.Yet the used fermented bacterium of these patents is microorganisms such as yeast and lactic acid brevibacterium at present, does not relate to Paecilomyces varioti fermentation vinasse and produces the report of green feed additive method.In a word, this patent and other patent differences: (1) used bacterial classification is different; (2) used culture medium difference; (3) production technology difference; (4) green feed additive composition of Sheng Chaning and function difference to some extent.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing vinasse fermentation to produce livestock birds health cultivation green feed additive, adopt Paecilomyces varioti fermentation vinasse, improve wherein concentrations of nutrient, reduce some material that is unfavorable for growth of animal in the vinasse, thereby realize the higher value application of brewing industry waste residue.
As everyone knows, feed product is converted into animal product and enters human food chain by feeding animals, and food security in the feed safety association.The inventor recognizes in scientific research: feed safety is the emphasis and the guarantee of healthy aquaculture; Development, development and application health cultivation green feed additive then are the guarantees of feed safety, are feed additive industry and feed industry development in future direction.And the key of exploitation health cultivation green feed additive is the residual and resistance to the action of a drug problem that solves antibiotic, synthetic drug, overcomes institute's toxic side effect of antibiotic etc additive.Green feed additive has following feature: 1. Nantural non-toxic side effect, noresidue, the no resistance to the action of a drug; 2. can promote growth of animals or poultry, can effectively and economically improve the production performance of livestock and poultry, improve efficiency of feed utilization and product quality, the culture benefit height; 3. can strengthen body's immunity, can prevent various infectiousness (viral, the bacillary) disease of animal and can adjust body physiological function; 4. use the back unharmful substance residual, do not influence the animal products quality, do not influence environment for human survival with healthy; 5. physicochemical property or biologically active stable in properties can enter intestines and stomach effectively and play a role, and do not influence the feed palatability; 6. share with the other drug additive, do not take place or rare incompatibility, bacterium is difficult for developing immunity to drugs to it; 7. improve the animal anti-stress ability; 8. improve environment.
This seminar studies show that, existing 64 effective taxons, wherein most of fall ill certainly insect and the soil of separating of only comprising of global paecilomyces (Paecilomyces Bain.).The phorozoon of Chinese caterpillar fungus partly is the paecilomyces fungi, kind of nucleosides material surplus the big multipotency of paecilomyces fungi produces 10 (as adenosine and 3 '-desoxyadenossine), sweet mellow wine, fungi polysaccharide, 20 seed amino acids etc., so have very high nutritive value and health care.Not only can be used as nutritious supplementary pharmaceutical and the natural antibiotics (some nucleosides material, antibacterial peptide) of animal, can also improve the immunologic function (effects of compositions such as fungi polysaccharide) of animal.Significantly reduce the death rate of weanling pig and livestock and poultry, can realize not medication and few medication.
By the test of many times checking, the method for utilizing vinasse fermentation to produce livestock birds health cultivation green feed additive of inventor's exploitation comprises:
1. vinasse batch mixing packing
The distillers ' grains that has just taken the dish out of the pot is divided in nontoxic, the tasteless ensiling polybag, and places in the aseptic fermenting cellar that means such as prior employing ultraviolet sterilization carry out disinfection stand-by.
2. medium sterilization
Adopt ClO 2 solution that potale culture medium is sterilized: with mass fraction is that 0.05% chlorine dioxide temperature boiling water sprays into the polybag of filling vinasse fast with aseptic sprayer unit, treat to tie sack after ClO 2 solution infiltrates vinasse fully, place 2h and promptly inoculate.
3. liquid spawn is made
(1) original strain is taken out from 4 ℃ of refrigerators, aseptic inoculation is on the PDA slant medium;
(2) inclined-plane of inoculation Paecilomyces varioti places dark constant temperature culture, waits bacterial classification to cover with the inclined-plane, makes spore suspension with aseptic washing inclined-plane;
(3) the Paecilomyces varioti spore suspension is diluted to suitable concn, then spore suspension is inserted in the liquid mother culture media, shaking table is cultivated and is promptly got liquid spawn.
4. inoculation fermentation
Press the liquid spawn of the inoculum concentration inoculation Paecilomyces varioti of mass fraction 5%, tie sack and place dark constant temperature culture, can stop fermentation after treating to cover with on the vinasse mycelia.
5. dry also pulverizing
The vinasse that cover with mycelia are disperseed the back low temperature drying, pulverize the back packing and be finished product.
In second step of said method, ClO 2 solution concentration is 0.05%, and the ClO 2 solution consumption a small amount of ponding occurs with the vinasse bottom and is advisable.
In the 3rd step of said method, the inclined-plane constant temperature culture temperature of described Paecilomyces varioti is 22 ℃, 5 days time; The temperature that shaking table is cultivated is 20 ℃～28 ℃, and rotating speed is 150r/min, time 3d; The prescription of described liquid mother culture media is a peptone 2%, sucrose 2%, MgSO 47H 2O 0.05%, KH 2PO 40.1%, water 1000mL, pH nature; Liquid amount is the 500mL/1000mL triangular flask.
In the 4th step of said method, described dark constant temperature culture temperature is 20 ℃～25 ℃, and incubation time is 10d～12d.
In the 5th step of said method, described bake out temperature is 50 ℃.
The inventor points out: with Paecilomyces varioti vinasse are fermented, owing to be filled with uniform a certain amount of husk in the vinasse, can guarantee oxygen needs of mycelial growth, there is the space of abundance allow mycelial growth again, so that tie the sack fermentation, prevent the open type fermented pollution that brings.
The inventor points out again: use the sterilizing methods of chlorine dioxide as potale culture medium, but both energy savings can also reduce the destruction of high-temperature sterilization to the vinasse active component.Chlorine dioxide has the characteristics of high efficiency, security and broad spectrum activity on food sterilizing is sterilized, be present internationally recognized optimal antibacterial disinfectant, and Ministry of Public Health's approved chlorine dioxide is disinfectant and new food additive (GB2760-1996).
The inventor also points out: utilize Paecilomyces varioti bacterial strain fermentation distillers ' grains to produce green feed additive, have raw material sources wide, easily produce, quality controllable, with low cost, characteristics such as be widely used.
The present invention is that matrix is fermented with vinasse with the Paecilomyces varioti that is separated in the soil and the insect of falling ill first, and carry out pathology, toxicity and pharmacological research, carry out feeding experiment, apply it in the animal and fowl fodder, develop a kind of novel cheap livestock birds health cultivation green feed additive.
The feed addictive protein content that the method is produced brings up to 26.8%～30.5% from 17%, and energy value is brought up to 4250kCal/kg from original 2510kCal/kg, directly can utilize digestibility to bring up to 62% by 33%; Kind of nucleosides material surplus main component contains 10 (as adenosine and 3 '-desoxyadenossine), sweet mellow wine, fungi polysaccharide, hepatocuprein (SOD), amino acid, sterol, alkaloid, VE, VB1, VB2 and mineral matter etc.Zoopery shows that this green feed additive action and efficacy is extensive:
(1) strengthens animal appetite, efficient growth promotion.
(2) improve food conversion ratio: the cordycepin in this feed, cordycepic acid etc. can effectively promote the growth of beneficial bacterium in the animal intestinal, improve food conversion ratio the control livestock and poultry diarrhea.
(3) significantly improve animal meat and local flavor: this green feed can reduce the cholesterol of livestock and poultry meat egg.Pork pig back fat fat and poultry stomach fat thickness are reduced, and lean meat percentage obviously improves; Improve meat and local flavor, eliminate the urine smell flavor, significantly increase fat content between flesh, tender degree of meat and bright fragrance strengthen.
(4) performance is powerful anti-stress effect: can effectively prevent and treat the livestock and poultry dysphoria, sting tail, fight, feather picking etc. stress syndrome, and can regulate the animal nerve function, eliminate allotriophagy.
(5) improve immunologic function: improve the animal blood circulation, improve livestock and poultry immunity of organisms and premunition; Make livestock and poultry to stress environment and living environment produce better resistance, control piglet and young fowl dysentery and diarrhoea; Significantly reduce weanling pig and livestock and poultry death rate, can realize not medication and few medication.
(6) Du Te physiology regulative mechanism:, improve production performance and the fertility of animal by digestion and absorption function of enhancing animal and the anabolism function in the body.
The specific embodiment
Embodiment 1:
It is 0.5m that the distillers ' grains that has just taken the dish out of the pot is divided in diameter, and length is in nontoxic, the tasteless ensiling polybag about 1m, and places in the aseptic fermenting cellar that means such as prior employing ultraviolet sterilization carry out disinfection stand-by; With mass fraction is that 0.05% chlorine dioxide temperature boiling water sprays into the polybag of filling vinasse fast with aseptic sprayer unit, treats to tie sack after ClO 2 solution infiltrates vinasse fully, places 2h and inoculates at once.The ClO 2 solution consumption ponding occurs with the vinasse bottom and is advisable.
Pupa is drawn up mould CGMCCNo.2459 bacterial strain, and (on April 17th, 2008 is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, the Latin classification called after Paecilomyces militaris of biological material specimens, depositary institution address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica) spore suspension is diluted to suitable concn, then spore suspension is inserted (peptone 2% in the liquid mother culture media, sucrose 2%, MgSO 47H 2O 0.05%, KH 2PO 40.1%, water 1000mL, pH nature; Liquid amount is the 500mL/1000mL triangular flask), under 20 ℃～28 ℃, cultivate 3d and promptly get liquid spawn with the rotating speed shaking table of 150r/min.
By the liquid spawn that the inoculum concentration inoculation pupa of mass fraction 5% is drawn up mould, tie sack and place 20 ℃～25 ℃ dark constant temperature culture 10d～12d down; Treat to stop fermentation after mycelia is covered with vinasse.The vinasse that cover with mycelia are disperseed to be placed in 50 ℃ the baking oven and dry, pulverize the back packing and be finished product.
Embodiment 2:
It is 0.5m that the distillers ' grains that has just taken the dish out of the pot is divided in diameter, and length is in nontoxic, the tasteless ensiling polybag about 1m, and places in the aseptic fermenting cellar that means such as prior employing ultraviolet sterilization carry out disinfection stand-by; With mass fraction is that 0.05% chlorine dioxide temperature boiling water sprays in the polybag of filling vinasse fast with aseptic sprayer unit, treats to tie sack after ClO 2 solution infiltrates vinasse fully, places a few hours and can inoculate.The ClO 2 solution consumption ponding occurs with the vinasse bottom and is advisable.
(on April 17th, 2008 is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation with thin pin Paecilomyces varioti CGMCCNo.2460 bacterial strain, the Latin classification called after Paecilomyces tenuipes of biological material specimens, depositary institution address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica) spore suspension is diluted to suitable concn, then spore suspension is inserted (medium component: peptone 2% in the liquid mother culture media, sucrose 2%, MgSO 47H 2O 0.05%, KH 2PO 40.1%, water 1000mL, pH nature; Liquid amount is the 500mL/1000mL triangular flask), under 20 ℃～28 ℃, cultivate 3d and promptly get liquid spawn with the rotating speed shaking table of 150r/min.
Inoculate the liquid spawn of thin pin Paecilomyces varioti by the inoculum concentration of mass fraction 5%, tie sack and put 20 ℃～25 ℃ dark constant temperature culture 10d～15d down; Treat to stop fermentation after mycelia is covered with vinasse.The vinasse that cover with mycelia are disperseed to be placed in 50 ℃ the baking oven and dry, pulverize the back packing and be finished product.

Claims (5)

1. utilize vinasse to produce the method for livestock birds health cultivation green feed additive, its feature comprises:
1. vinasse batch mixing packing
The distillers ' grains that has just taken the dish out of the pot is divided in nontoxic, the tasteless ensiling polybag, and places in the aseptic fermenting cellar that means such as prior employing ultraviolet sterilization carry out disinfection stand-by.
2. medium sterilization---usefulness chlorine dioxide is as the bactericidal agent of potale culture medium
Chlorine dioxide temperature boiling water is sprayed in the polybag of filling vinasse fast with aseptic sprayer unit, treat to tie sack after ClO 2 solution infiltrates vinasse fully, promptly inoculate after the placement.
3. liquid spawn is made
(1) original strain is taken out from 4 ℃ of refrigerators, aseptic inoculation is on the PDA slant medium;
(2) inclined-plane of inoculation Paecilomyces varioti places dark constant temperature culture, waits bacterial classification to cover with the inclined-plane, makes spore suspension with aseptic washing inclined-plane;
(3) the Paecilomyces varioti spore suspension is diluted to suitable concn, then spore suspension is inserted in the liquid mother culture media, shaking table is cultivated and is promptly got liquid spawn.
4. inoculation fermentation
The liquid spawn of inoculation Paecilomyces varioti ties sack and places the dark constant temperature culture, treats can stop fermentation after mycelia is covered with vinasse.
5. dry also pulverizing
The vinasse that cover with mycelia are disperseed the back low temperature drying, pulverize the back packing and be finished product.
2. method according to claim 1 is characterized in that the chlorine dioxide consumption is 0.05% of a vinasse quality in second step of method, and be 2h standing time.
3. method according to claim 1 is characterized in that the inclined-plane constant temperature culture temperature of described Paecilomyces varioti is 22 ℃ in the 3rd step of method, and the time is 5d; The temperature that shaking table is cultivated is 20 ℃～28 ℃, and rotating speed is 150r/min, time 3d; The prescription of described liquid mother culture media is a peptone 2%, sucrose 2%, MgSO 47H 2O 0.05%, KH 2PO 40.1%, water 1000mL, pH nature; Liquid amount is the 500mL/1000mL triangular flask.
4. method according to claim 1 is characterized in that described inoculum concentration counts 5% by mass fraction in the 4th step of method; Dark constant temperature culture temperature is 20 ℃～25 ℃, and incubation time is 10d～12d.
5. method according to claim 1 is characterized in that described bake out temperature is 50 ℃ in the 5th step of method.
CN2008100690834A 2008-12-29 2008-12-29 Method for producing livestock birds health cultivation green feed additive using distilled grain Expired - Fee Related CN101491290B (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103461648A (en) * 2013-09-23 2013-12-25 精晶药业股份有限公司 Method for preparing feed by virtue of secondary fermentation of amino acid fermentation hyphae
CN103947828A (en) * 2014-05-06 2014-07-30 安徽东方新新生物技术有限公司 Feed prepared by biologically fermenting white spirit vinasse and blood powder
CN105886430A (en) * 2016-04-27 2016-08-24 石家庄大众肥业有限公司 Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation
CN107019092A (en) * 2017-03-22 2017-08-08 燕淑海 The preparation method of agricultural industry chain based on persimmon
CN113583077A (en) * 2021-08-25 2021-11-02 宜宾五粮液股份有限公司 Process for preparing sterols

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103461648A (en) * 2013-09-23 2013-12-25 精晶药业股份有限公司 Method for preparing feed by virtue of secondary fermentation of amino acid fermentation hyphae
CN103947828A (en) * 2014-05-06 2014-07-30 安徽东方新新生物技术有限公司 Feed prepared by biologically fermenting white spirit vinasse and blood powder
CN105886430A (en) * 2016-04-27 2016-08-24 石家庄大众肥业有限公司 Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation
CN107019092A (en) * 2017-03-22 2017-08-08 燕淑海 The preparation method of agricultural industry chain based on persimmon
CN113583077A (en) * 2021-08-25 2021-11-02 宜宾五粮液股份有限公司 Process for preparing sterols
CN113583077B (en) * 2021-08-25 2022-09-16 宜宾五粮液股份有限公司 Process for preparing sterols

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