CN109576149A - Bacteria Culture detection device - Google Patents
Bacteria Culture detection device Download PDFInfo
- Publication number
- CN109576149A CN109576149A CN201811625558.3A CN201811625558A CN109576149A CN 109576149 A CN109576149 A CN 109576149A CN 201811625558 A CN201811625558 A CN 201811625558A CN 109576149 A CN109576149 A CN 109576149A
- Authority
- CN
- China
- Prior art keywords
- culture
- connector
- carbon dioxide
- bacteria
- culture bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to Bacteria Culture detection devices.The Bacteria Culture detection device, including culture bottle, connector, carbon dioxide inductor and computer.Filling culture medium in culture bottle, collects bacteria sample, and culture bottleneck is sealed equipped with butyl rubber plug.Culture bottle structure is simple, and production cost is low；Connector hollow design, lower end is equipped with puncture needle, puncture culture bottleneck butyl rubber plug, culture bottle cavity is set to pass through connector and carbon dioxide inductor connection, filter membrane is equipped in connector pipeline, the culture for anaerobism sample can set up oxygen consumption bag, it is equipped with oxygen consumption agent in bag, is conducive to the growth of anaerobic bacteria.Carbon dioxide sensing device is connected to connector, incude changes of concentrations of carbon dioxide state in culture bottle, judges Bacteria cold shock in culture solution, while carbon dioxide sensing device connects computer, carbon dioxide multidate information is analyzed, laboratory operation personnel are prompted to.
Description
Technical field
The present invention relates to biological sample Bacteria Culture and detection techniques, promote bacterial growth by certain cultivation cycle,
Bacteria growth process is recorded, and is detected by specific device.Therefore one kind is related to the contents of the present invention, i.e. novel fine
Bacterium cultivates detection device.
Background technique
During food hygiene and medical test, generally require to carry out Bacteria Culture and detection to sample.To find sample
Bacteria cold shock in this.Bacteria Culture and detection on the market at present is operated using manual method mostly.This behaviour
It is not easy to standardization and normalization as method, in particular by the influence of artifact, cultivation results are precisely different.
Summary of the invention
In order to solve the above problem, the present invention provide a kind of structure it is simple, can in time, directly sense in culture bottle have
Without bacterial growth, Bacteria cold shock is more sensitive to report bacterial growth information to the device of laboratory operation personnel.
The technical solution adopted by the present invention is that: novel bacterial culture detection device, including culture bottle, connector and titanium dioxide
Carbon inductor.It is characterized by: filling culture medium in the culture bottle, collects sample and injects culture bottle, culture bottleneck is equipped with fourth
The sealing of base rubber stopper, the connector lower end are equipped with puncture needle, and puncture needle can puncture butyl rubber bung and extend into culture bottle,
Connector hollow design, upper end and carbon dioxide inductor connection, hollow pipeline is interior to be equipped with bacteriological filter film.The carbon dioxide
Inductor is connected to connector inner passage, and connects computer.The connector upper end is equipped with carbon dioxide inductor, described
Carbon dioxide inductor is connected with detection needle tubing, and carbon dioxide inductor is connect with computer, and analysis titanium dioxide is automatically accepted in computer
The information change of carbon inductor.
The beneficial effect that the present invention obtains is: culture bottle structure is simple, and production cost is low；Bottle mouth position on culture bottle passes through
Puncture needle is connected to connector, and the upper end of connector is connected to carbon dioxide inductor, which can be direct
It detects changes of concentrations of carbon dioxide situation in culture bottle and report result is carried out by computer.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is the structural schematic diagram of culture bottle；
Fig. 3 is the structural schematic diagram of connector；
Fig. 4 is carbon dioxide sensing device structural schematic diagram.
Specific embodiment
The present invention will be further explained in the following with reference to the drawings and specific embodiments.
As shown in Figure 1, novel bacterial culture detection device of the invention, including culture bottle 1, connector 2 and carbon dioxide
Inductor 3.Filling culture medium in culture bottle 1 collects sample, and bottleneck is equipped with butyl rubber bung 4, and connector lower end is equipped with puncture needle
5, butyl rubber bung is punctured by puncture needle and is extend into culture bottle, carbon dioxide gas in culture bottle is made to pass through hollow connectors
Conduction to carbon dioxide inductor, carbon dioxide inductor connects computer, analyzes changes of concentrations of carbon dioxide situation, makes report
Alert prompt.
As shown in Fig. 2, culture bottle 1 described in detection report male device, filling fluid nutrient medium 10 cultivate sample.Bottle
Mouth is equipped with butyl rubber bung 4 and seals.
As shown in figure 3, connector 2 described in detection report male device, hollow design, lower end are equipped with puncture needle 5, puncture pin puncture
Butyl rubber plug 4 on culture bottle 1 is extend into culture bottle.Filter membrane 7 is equipped in connector hollow pipeline, to prevent culture bottle
Interior germ contamination culture environment.The upper end of connector 2 is connected to carbon dioxide inductor 3, by the carbon dioxide in culture bottle
Gas conduction is to carbon dioxide inductor 3.Culture for anaerobism sample, connector can set up oxygen consumption bag 8, in oxygen consumption bag 8
If oxygen consumption agent 9 is connected to connector, to consume the oxygen in connector pipeline, be conducive to the growth of anaerobic bacteria.
As shown in figure 4, carbon dioxide inductor 3 described in detection report male device and computer 11 connect, carbon dioxide induction
Device 3 is connected to by connector 2 with culture bottle 1, to acquire changes of concentrations of carbon dioxide information in culture bottle.Computer 11 is used for
Changes of concentrations of carbon dioxide information is analyzed, bacterial growth information is notified at once to operator.
Claims (2)
1. a kind of Bacteria Culture detection device, including culture bottle (1), connector (2), carbon dioxide inductor (3) and computer
(11), it is characterised in that: filling fluid nutrient medium (10) in the culture bottle (1) collects bacteria sample；The culture bottle (1)
Bottle mouth position is equipped with butyl rubber plug (4) to seal culture bottle (1)；Connector (2) hollow design, connector (2) lower end are equipped with
Puncture needle (5), puncture needle (5) puncture the butyl rubber plug (4) of culture bottle (1) bottle mouth position, connector (2) upper end and carbon dioxide sense
Device (3) are answered to be connected to, carbon dioxide connector (3) is connect with computer (11)；Connector passage is equipped with filter membrane (7).
2. bacteria detecting apparatus according to claim 1, it is characterised in that: the connector (2) sets up oxygen consumption bag (8),
Oxygen consumption agent (9) are equipped in oxygen consumption bag (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811625558.3A CN109576149A (en) | 2018-12-28 | 2018-12-28 | Bacteria Culture detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811625558.3A CN109576149A (en) | 2018-12-28 | 2018-12-28 | Bacteria Culture detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109576149A true CN109576149A (en) | 2019-04-05 |
Family
ID=65932262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811625558.3A Pending CN109576149A (en) | 2018-12-28 | 2018-12-28 | Bacteria Culture detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109576149A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699480A (en) * | 2017-11-06 | 2018-02-16 | 武汉迪艾斯科技有限公司 | A kind of blood culture detection report male device |
| CN207845652U (en) * | 2017-12-27 | 2018-09-11 | 武汉迪艾斯科技有限公司 | A kind of blood culture connector |
-
2018
- 2018-12-28 CN CN201811625558.3A patent/CN109576149A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699480A (en) * | 2017-11-06 | 2018-02-16 | 武汉迪艾斯科技有限公司 | A kind of blood culture detection report male device |
| CN207845652U (en) * | 2017-12-27 | 2018-09-11 | 武汉迪艾斯科技有限公司 | A kind of blood culture connector |
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| SE01 | Entry into force of request for substantive examination | ||
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