CN109576130A - Artificially manufacture the device that micro- oxygen environment purifies micro- oxygen bacterium - Google Patents
Artificially manufacture the device that micro- oxygen environment purifies micro- oxygen bacterium Download PDFInfo
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- CN109576130A CN109576130A CN201811547909.3A CN201811547909A CN109576130A CN 109576130 A CN109576130 A CN 109576130A CN 201811547909 A CN201811547909 A CN 201811547909A CN 109576130 A CN109576130 A CN 109576130A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
Abstract
Artificially manufacture the device that micro- oxygen environment purifies micro- oxygen bacterium, belong to technical field of microbiology, by substrate (1), cover plate (2), sealing compound (3), solid medium (4), label paper (5) composition, it is characterized by: substrate (1) is the preferable nature of glass of transparency, in cuboid, including bracket (6), groove (7), opening (8), label area (9), bracket (6) are had on substrate (1), bracket (6) is processed by glass factory, for cuboid frame, the region that bracket (6) surrounds is groove (7), groove (7) is for holding solid medium (4), the top of bracket (6) is opening (8), opening (8) is bonded using sealing compound (3) and cover plate (2), label area (9) is rectangle, for adhesive label paper (5), label Fill message on paper (5), device production is simple, and strong operability is low in cost, and effect is obvious.
Description
Technical field
The present invention relates to one kind artificially to manufacture the device that micro- oxygen environment purifies micro- oxygen bacterium, belongs to microbiological technique neck
Domain.
Background technique
The purifying of micro- oxygen bacterium is a kind of skill that the micro- oxygen bacterium acquired under the conditions of micro- oxygen environment is further purified
Art.Micro- oxygen bacterium refer to no outside air supply under conditions of, merely in device remaining oxygen survive a kind of bacterium,
Environment locating for this kind of bacterium is referred to as micro- oxygen environment.Bacterium is widely distributed in nature, and type is more, and purposes is wide.Bacterium
Purifying is purified to the bacterium turned out, and the function etc. of bacterium is further identified and played.Looked into patent and
Document, the purification devices not to the bacterium turned out under micro- oxygen environment, such as the bacterium of enterobacteriaceae and deep subsoil.How will
It is to carry out applying in purification of bacterial experiment under micro- oxygen environment and promote a disaster urgently to be solved that obtained bacterium, which carries out purifying,
Topic purifies micro- oxygen bacterium under micro- oxygen environment thin to play so a kind of device for purifying micro- oxygen bacterium must be found
The function of bacterium.Therefore, using solid medium is placed on standoff substrate, the space in groove is reduced, waits culture
It is tested after base solidification, crosses on solid medium and micro- oxygen bacterium is purified, then above by cover plate lid, with envelope
Chewing-gum clings the gap between substrate and cover plate, prevents outside air from entering, so that reaching a kind of artificially manufactures micro- oxygen environment
The device of micro- oxygen bacterium is purified, and then solves the issues of purification to the bacterium obtained under micro- oxygen environment.Invent a kind of artificial system
It makes micro- oxygen environment and purifies the device of micro- oxygen bacterium and be necessary.
Summary of the invention
In order to overcome the problems, such as how to purify the micro- oxygen bacterium obtained under the conditions of micro- oxygen environment, the present invention provides artificial
The device that micro- oxygen environment purifies micro- oxygen bacterium is manufactured, it is to utilize that this kind, which artificially manufactures micro- oxygen environment and purify the device of micro- oxygen bacterium,
With solid medium is placed on standoff substrate, the space in groove is reduced, culture medium solidification is waited to incite somebody to action when being tested,
Scribing line purifies micro- oxygen bacterium on solid medium, then above by cover plate lid, with sealing compound by substrate and cover plate it
Between gap cling, prevent outside air from entering, provide a special micro- oxygen environment condition to purify micro- oxygen bacterium, and then solve
Certainly purify the difficulty of micro- oxygen bacterium.
The technical solution adopted by the present invention to solve the technical problems is:
1. the present invention artificially to manufacture the device that micro- oxygen environment purifies micro- oxygen bacterium, by substrate 1, cover plate 2, sealing compound 3, consolidates
Body culture medium 4, label paper 5 form, it is characterised in that: and the substrate 1 is the preferable nature of glass of transparency, is in cuboid,
Length is 60-80 millimeters, and width is 30-40 millimeters, and with a thickness of 1-3 millimeters, substrate 1 includes bracket 6, groove 7, opening 8, mark
Area 9 is signed, bracket 6 is had on substrate 1, it be length is 30-50 millimeter that bracket 6 is processed by glass factory, and width is 20-30 millimeters, height
Degree is 1-3 millimeters, and with a thickness of 3-5 millimeters of cuboid frame, the region that bracket 6 surrounds is groove 7, and groove 7 is for holding
Solid medium 4, volume are 0.3-3.1 cubic centimetre, and the top of bracket 6 is opening 8, opening 8 using sealing compound 3 and
Cover plate 2 bonds, and label area 9 is rectangle, and length is 25-30 millimeters, and width is 20-25 millimeters, is used for adhesive label paper 5, mark
Sample message, kinds of culture medium, operator's name and the Production Time of culture are filled on label paper 5.
The groove 7 is being formed by micro- oxygen ring for it for holding solid medium 4, and after being inoculated with micro- oxygen bacterium
It is purified in border, solid medium 4 is used exclusively for purifying the culture medium of micro- oxygen bacterium, and 7 volume of groove is 0.3-3.1 cubes
Centimetre, solid medium 4 is fitted into groove 7, and liquid level is 0.2-0.5 millimeters at a distance from opening 8.
The cover plate 2 is the nature of glass, is in cuboid, and length is 30-50 millimeters, and width is 20-30 millimeters, with a thickness of
1-3 millimeters, lid on the substrate 1, is clung the gap between opening 8 and cover plate 2 with sealing compound 3, sealing compound 3 is well known heat
Melten gel, the basis of hot melt adhesive are resins made of ethylene and vinyl acetate are copolymerized at high temperature under high pressure, in rodlike, are being heated
The tip of glue rifle can melt.
The operating method for artificially manufacturing micro- oxygen environment and purifying the device of micro- oxygen bacterium are as follows: on superclean bench,
Substrate 1 and cover plate 2 are subjected to disinfecting action, are placed on after sterilizing spare on superclean bench；According to the specific requirement of purpose bacterium, match
Solid medium 4 processed, is held after sterilizing with vial, is put into spare on superclean bench；100,1000 microlitres of pipette tips are gone out
Bacterium is put into spare in the pipette tips box on superclean bench；Thermosol gelgun and 75% alcohol of rodlike sealing compound 3 are sprayed,
It is put into spare in the glue stick box on superclean bench；75% alcohol of the device for filling bacterium to be purified is sprayed, is put into super
It is spare on net workbench；Label paper 5 after sterilizing is put on superclean bench, after cooling, label paper 5 taken off, is pasted onto base
The label area 9 of piece 1 is spare；A marking pen is taken, is sprayed, is put into spare in the penholder on superclean bench with 75% alcohol；
Liquid-transfering gun and 75% alcohol of oese are sprayed, are put into spare on the pipette holder on superclean bench；When operation, base is taken
Piece 1, is placed on superclean bench, after 100,1000 microlitres of pipette tips of liquid relief holster, draws solid medium 4, injects groove 7
It is interior, when solid medium 4 when it is 0.2-0.5 millimeters at a distance from opening 8 that the additive amount of groove 7, which reaches liquid level, stop adding
Add, solid medium 4 is waited to start to test when solidifying, the bacterium cultivated under micro-oxygen conditions is then purified using oese, it will
Oese is baked 1-2 minutes under alcolhol burner, until oese reddens, is waited oese to cool down, is dipped a small amount of bacterium solution, is trained in solid
It supports the lower section of the left end of base 4 and gently the vertical line of standardized item or draws two vertical parallel lines, oese is placed on wine again
It is baked 1-2 minute on smart lamp, until redden, waiting oese is cooled down, and a small amount of bacterium solution is dipped, perpendicular to drawing to obtain the last item line just now
End at start to cross, gently the line of standardized item level or draw two horizontal parallels line, then oese is placed on again
It is baked 1-2 minute on alcolhol burner, until redden, waiting oese is cooled down, and a small amount of bacterium solution is dipped, perpendicular to drawing to obtain the last item just now
Start to cross at the end of line, gently the vertical line of standardized item or draw two vertical parallel lines, until by entire solid culture
Base 4 is full of scribing line；It takes cover plate 2 to cover in opening 8, picks and places the thermosol gelgun for having rodlike sealing compound 3, glue is coated in opening
Around place 8, after cooling cover plate 2 is adhered in the opening 8 of substrate 1, by sample message, kinds of culture medium, operator
Name and Production Time write on label paper 5, and the device that manufacture purifies micro- oxygen bacterium completes, and in order to repeat, every kind to be measured
3-6 substrate of sample making 1, later according to the characteristics of sample and the bacterium of purifying of the same race is respectively put into 4 DEG C, 10 by experiment purpose
DEG C, cultivated in the incubators of 15 DEG C, 20 DEG C, 30 DEG C, 37 DEG C different temperatures gradients, after separated in time, device is taken
Identification is further processed to bacterium in the bacterium purified out.
Beneficial effects of the present invention are that artificially to manufacture micro- oxygen environment to purify the device of micro- oxygen bacterium be using pasting cover plate
On with standoff substrate, micro- oxygen environment is artificially manufactured, solid medium is added in the groove of bracket, is reduced in groove
Space starts to test, crosses on solid medium and purify to micro- oxygen bacterium, with sealing when waiting solid medium solidification
Cover plate is pasted on substrate by glue, and ambient atmos are not into coming after sealing, to reach micro- oxygen that artificial manufacture purifies micro- oxygen bacterium
Environment carries out purifying for some bacteriums cultivated under micro- oxygen environment and provides a suitable purifying space, and then solves purifying
The difficulty of micro- oxygen bacterium.It artificially manufactures micro- oxygen environment and purifies the production of device used in micro- oxygen bacterium simply, strong operability, cost
Cheap, effect is obvious.
Detailed description of the invention
The following further describes the present invention with reference to the drawings.
Fig. 1 is that the present inventor is the overall structure diagram for manufacturing micro- oxygen environment and purifying micro- oxygen bacterium device.
Fig. 2 is that the present inventor is the overall structure diagram for manufacturing the bracket that micro- oxygen environment purifies micro- oxygen bacterium device.
1. substrate in figure, 2. cover plates, 3. sealing compounds, 4. solid mediums, 5. label papers, 6. brackets, 7. grooves, 8. openings
Place, 9. label areas.
Specific embodiment
Embodiment one:
As shown, the present invention is artificially to manufacture the device that micro- oxygen environment purifies micro- oxygen bacterium, by substrate 1, cover plate 2, envelope
Chewing-gum 3, solid medium 4, label paper 5 form, and substrate 1 is the preferable nature of glass of transparency, are in cuboid, length 60-
80 millimeters, width is 30-40 millimeters, and with a thickness of 1-3 millimeters, substrate 1 includes bracket 6, groove 7, opening 8, label area 9, base
Bracket 6 is had on piece 1, it be length is 30-50 millimeter that bracket 6 processed by glass factory, and it is highly 1-3 that width, which is 20-30 millimeters,
Millimeter, with a thickness of 3-5 millimeters of cuboid frame, the region that bracket 6 surrounds is groove 7, and groove 7 is for holding solid culture
Base 4, volume are 0.3-3.1 cubic centimetres, and the top of bracket 6 is opening 8, and opening 8 is viscous using sealing compound 3 and cover plate 2
It closes, label area 9 is rectangle, and length is 25-30 millimeter, and width is 20-25 millimeters, for adhesive label paper 5, on label paper 5
Fill in sample message, kinds of culture medium, operator's name and the Production Time of culture.Groove 7 is used to hold solid medium 4,
And purified in being formed by micro- oxygen environment after being inoculated with micro- oxygen bacterium for it, solid medium 4 is used exclusively for purifying
The culture medium of micro- oxygen bacterium, 7 volume of groove are 0.3-3.1 cubic centimetres, and solid medium 4 is fitted into groove 7, liquid level and opening
The distance at place 8 is 0.2-0.5 millimeters.Cover plate 2 is the nature of glass, is in cuboid, and length is 30-50 millimeters, and width is 20-30 milli
Rice, with a thickness of 1-3 millimeters, lid on the substrate 1, is clung the gap between opening 8 and cover plate 2 with sealing compound 3, sealing compound 3
It is well known hot melt adhesive, the basis of hot melt adhesive is resin made of ethylene and vinyl acetate are copolymerized at high temperature under high pressure, is in
It is rodlike, it can be melted at the tip of thermosol gelgun.Solid medium 4 is the monoid according to the bacterium cultivated and particular formulation,
Its ingredient is tryptone 10.0g/L, yeast extract 3.0g/L, sodium chloride 9.0g/L, ammonium chloride 0.15g/L, dipotassium hydrogen phosphate
0.3g/L, potassium dihydrogen phosphate 0.2g/L, agar 17.0g/L, pH value is 7.0-7.2 at room temperature, when configuration, is distinguished with assay balance
Weigh tryptone 10.0g, yeast extract 3.0g, sodium chloride 9.0g, ammonium chloride 0.15g, dipotassium hydrogen phosphate 0.3g, biphosphate
1000 milliliters of distilled water are added in potassium 0.2g, at room temperature with pH meter adjust solution ph, pH value between 7.0-7.2,
Agar 17.0g is weighed with assay balance again after adjusting pH value to be added in culture medium, and 100,1000 milliliters of pipette tips are covered and are being moved
On liquid device, solid medium 4 is drawn when the temperature of solid medium 4 is at 70 DEG C or so and does not solidify and is injected into groove 7
In, since solid medium 4 is solid-state containing agar, so the additive amount of solid medium 4 reaches liquid level opening in groove 7
Place 8 distance be 0.2-0.5 millimeter when stopping add, this is because if solid medium 4 added in groove 7 it is too full,
It is easy to cause culture medium and sealing compound 3 to admix when sealing, opening 8 is poorly sealed close；If solid medium 4 is in groove 7
Add less, although being easy that culture medium and sealing compound 3 is avoided to admix when sealing, the remaining oxygen of groove 7 is then excessive, up to not
To the purpose artificially to manufacture micro- oxygen environment.Sealing compound 3 is well known hot melt adhesive, i.e., a kind of adhesive of plasticity, certain
Its physical state changes with temperature and is changed in temperature range, and chemical property is constant, non-toxic and tasteless, belongs to environmental-protecting chemical and produces
Product, the basis of hot melt adhesive are resins made of ethylene and vinyl acetate are copolymerized at high temperature under high pressure, early stage viscosity and later period
It is sticky consistent, in rodlike, can be melted at the tip of thermosol gelgun, be applied to opening 8, cover plate 2 can be pasted after cooling and
It fixes on the substrate 1.The operating method for artificially manufacturing micro- oxygen environment and purifying the device of micro- oxygen bacterium are as follows: by 1 He of substrate
Cover plate 2 carries out disinfecting action, is placed on after sterilizing spare on superclean bench；According to the specific requirement of purpose bacterium, solid training is prepared
Base 4 is supported, weighs tryptone 10.0g, yeast extract 3.0g, sodium chloride 9.0g, ammonium chloride 0.15g, phosphorus respectively with assay balance
Sour hydrogen dipotassium 0.3g, potassium dihydrogen phosphate 0.2g, are added 1000 milliliters of distilled water, adjust solution ph, pH with pH meter at room temperature
Value weighs agar 17.0g with assay balance again after adjusting pH value and is added in culture medium, go out between 7.0-7.2
It is held, is put into spare after cooling on superclean bench with vial after bacterium；100,1000 microlitres of pipette tips are sterilized, are put into ultra-clean
It is spare in pipette tips box on workbench；Thermosol gelgun and 75% alcohol of rodlike sealing compound 3 are sprayed, ultra-clean work is put into
It is spare in glue stick box on platform；75% alcohol of the device for filling bacterium to be purified is sprayed, is put on superclean bench
It is spare in glue stick box；Label paper 5 after sterilizing is put on superclean bench, after cooling, label paper 5 taken off, is pasted onto substrate 1
Label area 9 it is spare；A marking pen is taken, is sprayed, is put into spare in the penholder on superclean bench with 75% alcohol；It will move
Liquid rifle and oese are sprayed with 75% alcohol, are put into spare on the pipette holder on superclean bench；When operation, substrate 1 is taken,
It is placed on superclean bench, with a liquid-transfering gun, after putting on 100,1000 microlitres of pipette tips, draws solid medium 4, inject groove
In 7, stop addition when the distance that the additive amount of groove 7 reaches liquid level opening 8 is 0.2-0.5 millimeters when solid medium 4,
It waits solid medium 4 to start to test when solidifying, the bacterium cultivated under micro-oxygen conditions is then purified using oese, will be inoculated with
Ring is baked 1-2 minutes under alcolhol burner, until oese reddens, is waited oese to cool down, a small amount of bacterium solution is dipped, in solid medium
Two vertical parallel lines gently the vertical line of standardized item or are drawn in the lower section of 4 left end, and oese is placed on alcolhol burner again
Upper roasting 1-2 minute, until reddening, waiting oese was cooled down, and dipped a small amount of bacterium solution, perpendicular to drew just now the last item line end
Start to cross at tail, gently the line of standardized item level or the line of stroke two horizontal parallels, are then placed on alcohol for oese again
It is baked 1-2 minute on lamp, until redden, waiting oese cools down, and a small amount of bacterium solution is dipped, perpendicular to drawing to obtain the last item line just now
Start to cross at end, gently the vertical line of standardized item or stroke two vertical parallel lines, fill until by entire solid medium 4
Full scribing line；It takes cover plate 2 to cover in opening 8, picks and places the thermosol gelgun for having rodlike sealing compound 3, glue is coated in opening 8 weeks
Enclose, after cooling cover plate 2 be adhered in the opening 7 of substrate 1, by sample message, kinds of culture medium, operator's name and
Production Time writes on label paper 5, and the device that manufacture purifies micro- oxygen bacterium completes, in order to repeat, every kind of sample to be tested system
Make 3-6 substrate 1, later according to the characteristics of sample and the bacterium of purifying of the same race is respectively put into 4 DEG C, 10 DEG C, 15 by experiment purpose
DEG C, cultivated in the incubators of 20 DEG C, 30 DEG C, 37 DEG C different temperatures gradients, after separated in time, device is taken out to obtain
Identification is further processed to bacterium in the bacterium of purifying.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it will be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this hairs
Bright principle, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these variations
It all fall within the protetion scope of the claimed invention with improvement, its is equivalent by appended claims for the claimed scope of the invention
Object defines.
Claims (4)
1. people is the device that the micro- oxygen environment of manufacture purifies micro- oxygen bacterium, by substrate (1), cover plate (2), sealing compound (3), solid culture
Base (4), label paper (5) composition, it is characterised in that: the substrate (1) is the preferable nature of glass of transparency, is in cuboid,
Length is 60-80 millimeters, and width is 30-40 millimeters, and with a thickness of 1-3 millimeters, substrate (1) includes bracket (6), groove (7), opening
Locate (8), label area (9), have bracket (6) on substrate (1), it be length is 30-50 millimeters that bracket (6) is processed by glass factory, wide
Degree is 20-30 millimeters, is highly 1-3 millimeters, and with a thickness of 3-5 millimeters of cuboid frame, the region that bracket (6) surrounds is recessed
Slot (7), for groove (7) for holding solid medium (4), volume is 0.3-3.1 cubic centimetres, and the top of bracket (6) is to open
At mouthful (8), opening (8) is bonded using sealing compound (3) and cover plate (2), and label area (9) are rectangle, and length is 25-30 milli
Rice, width are 20-25 millimeters, are used for adhesive label paper (5), sample message, the culture medium kind of culture are filled on label paper (5)
Class, operator's name and Production Time.
2. the device according to claim 1 for artificially manufacturing micro- oxygen environment and purifying micro- oxygen bacterium, it is characterised in that: described
Groove (7) is carried out for it after being inoculated with micro- oxygen bacterium pure in being formed by micro- oxygen environment for holding solid medium (4)
To change, solid medium (4) is used exclusively for purifying the culture medium of micro- oxygen bacterium, and groove (7) volume is 0.3-3.1 cubic centimetres,
Solid medium (4) is fitted into groove (7), and liquid level is 0.2-0.5 millimeters at a distance from opening (8).
3. the device according to claim 1 for artificially manufacturing micro- oxygen environment and purifying micro- oxygen bacterium, it is characterised in that: described
Cover plate (2) is the nature of glass, is in cuboid, and length is 30-50 millimeter, and width is 20-30 millimeters, with a thickness of 1-3 millimeters, Gai
On substrate (1), the gap between opening (8) and cover plate (2) is clung with sealing compound (3), sealing compound (3) is well known hot melt
Glue, the basis of hot melt adhesive are resins made of ethylene and vinyl acetate are copolymerized at high temperature under high pressure, in rodlike, in hot melt adhesive
The tip of rifle can melt.
4. the operating method according to claim 1 for artificially manufacturing micro- oxygen environment and purifying the device of micro- oxygen bacterium are as follows: super
On net workbench, substrate (1) and cover plate (2) are subjected to disinfecting action, are placed on after sterilizing spare on superclean bench；According to purpose
The specific requirement of bacterium prepares solid medium (4), is held, be put into spare on superclean bench with vial after sterilizing；By 100,
1000 microlitres of pipette tips sterilizing, is put into spare in the pipette tips box on superclean bench；By thermosol gelgun and rodlike sealing compound (3)
It is sprayed, is put into spare in the glue stick box on superclean bench with 75% alcohol；The device for filling micro- oxygen bacterium to be purified is used
75% alcohol spraying, is put into spare on superclean bench；Label paper (5) after sterilizing is put on superclean bench, it is cooling
Afterwards, label paper (5) are taken off, the label area (9) for being pasted onto substrate (1) is spare；A marking pen is taken, is sprayed with 75% alcohol,
It is put into spare in the penholder on superclean bench；Liquid-transfering gun and 75% alcohol of oese are sprayed, superclean bench is put into
On pipette holder on it is spare；When operation, substrate (1) is taken, is placed on superclean bench, liquid-transfering gun puts on 100,1000 microlitres of rifles
It after head, draws solid medium (4), in injection groove (7), the additive amount to solid medium (4) in groove (7) reaches liquid level
With when at a distance from opening (8) being 0.2-0.5 millimeters, stop addition, starts to test until solid medium (4) solidification, connect
Purify the bacterium cultivated under the conditions of micro- oxygen environment using oese, oese is baked 1-2 minute under alcolhol burner, up to connecing
Kind ring reddens, and waits oese to cool down, dips a small amount of bacterium solution, gently standardized upwards below the left end of solid medium (4)
The vertical line of item draws two vertical parallel lines, oese is placed on alcolhol burner again and is baked 1-2 minutes, until redden, etc.
Ring to be seeded cools down, and dips a small amount of bacterium solution, perpendicular to drew just now the last item line end at start to cross, gently standardized item
Oese, is then placed on alcolhol burner again and bakes 1-2 minutes by the line of horizontal line or stroke two horizontal parallels, until redden,
Wait oese cool down, dip a small amount of bacterium solution, perpendicular to drew just now the last item line end at start to cross, it is gently standardized
The vertical line of item draws two vertical parallel lines, until entire solid medium (4) are full of scribing line；Cover plate (2) is taken to cover
In opening (8), the thermosol gelgun for there are rodlike sealing compound (3) is picked and placed, glue is coated in around opening (8), is made after cooling
It obtains cover plate (2) to be adhered in the opening (8) of substrate (1), by sample message, kinds of culture medium, operator's name and production
Time writes on label paper (5), and the device that manufacture purifies micro- oxygen bacterium completes, in order to repeat, every kind of sample to be tested production
3-6 substrate (1), later according to the characteristics of sample and the bacterium of purifying of the same race is respectively put into 4 DEG C, 10 DEG C, 15 by experiment purpose
DEG C, cultivated in the incubators of 20 DEG C, 30 DEG C, 37 DEG C different temperatures gradients, after separated in time, device is taken out to obtain
Identification is further processed to bacterium in the bacterium of purifying.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811547909.3A CN109576130A (en) | 2018-12-18 | 2018-12-18 | Artificially manufacture the device that micro- oxygen environment purifies micro- oxygen bacterium |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811547909.3A CN109576130A (en) | 2018-12-18 | 2018-12-18 | Artificially manufacture the device that micro- oxygen environment purifies micro- oxygen bacterium |
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| CN107532121A (en) * | 2015-04-29 | 2018-01-02 | 3M创新有限公司 | Culture apparatus for anaerobe |
| CN107312702A (en) * | 2017-08-23 | 2017-11-03 | 杭州至高生物科技有限公司 | A kind of culture based devices and cultural method with anaerobic device |
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