CN109557074A - A kind of acquisition method of Raman high spectrum image - Google Patents
A kind of acquisition method of Raman high spectrum image Download PDFInfo
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- CN109557074A CN109557074A CN201910021928.0A CN201910021928A CN109557074A CN 109557074 A CN109557074 A CN 109557074A CN 201910021928 A CN201910021928 A CN 201910021928A CN 109557074 A CN109557074 A CN 109557074A
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- 238000001069 Raman spectroscopy Methods 0.000 title claims abstract description 41
- 238000001228 spectrum Methods 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000004929 transmission Raman spectroscopy Methods 0.000 claims abstract description 14
- 230000005540 biological transmission Effects 0.000 claims abstract description 12
- 230000003287 optical effect Effects 0.000 claims abstract description 11
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 abstract description 20
- 238000012360 testing method Methods 0.000 abstract description 3
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- 238000003384 imaging method Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
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- 238000005516 engineering process Methods 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
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- 230000007246 mechanism Effects 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 229920000877 Melamine resin Polymers 0.000 description 1
- 238000001237 Raman spectrum Methods 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 238000000701 chemical imaging Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- JDSHMPZPIAZGSV-UHFFFAOYSA-N melamine Chemical compound NC1=NC(N)=NC(N)=N1 JDSHMPZPIAZGSV-UHFFFAOYSA-N 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 238000009659 non-destructive testing Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/2823—Imaging spectrometer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/42—Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4412—Scattering spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/42—Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
- G01J2003/423—Spectral arrangements using lasers, e.g. tunable
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/42—Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
- G01J2003/425—Reflectance
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N2021/653—Coherent methods [CARS]
- G01N2021/655—Stimulated Raman
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Abstract
The invention discloses a kind of acquisition methods of Raman high spectrum image, its main feature is that constituting the acquisition mode of reflection and transmission Raman high spectrum image using linear laser and reflective mirror and dichroscope, the Raman high spectrum image of sample to be tested is successively obtained, the dichroscope is arranged in the optical path between sample stage and light spectrum image-forming module；The reflective mirror reflects linear laser immediately below sample stage, and the acquisition with the Raman high spectrum image of horizontally disposed dichroscope composition transmission mode.The acquisition that there is the present invention single pass to be reflected and be transmitted two kinds of Raman high spectrum images compared with prior art, the shortcomings that not only overcoming single-point or multiple spot Raman spectrometer, a transmission Raman high light spectrum image-forming function is increased simultaneously, support can be provided to probe into the feature of the transmission Raman spectrum of object under test, so as to meet the application demand of different scenes.
Description
Technical field
The present invention relates to spectral imaging technology field, specifically a kind of acquisition method of Raman high spectrum image.
Background technique
Currently, Raman spectroscopy is in the side such as chemistry, high molecular material, material science, biomedicine, Analysis of Cultural Relic
Face is widely used.Raman spectrum has unique advantage in molecular spectrum research field, it can characterize object under test
Structural information, i.e. intramolecule normal mode vibration frequency and the case where related vibration level, to realize contained function in molecule
The identification of group, finally identifies molecular structure, Raman spectroscopy can be competent at qualitative analysis, structural analysis, quantitative analysis it is specific
Task.In view of the great market of Raman spectrometer, domestic and international relevant instrument company is all laid out in this aspect, including match
It is silent fly, must up to Tyke, Switzerland ten thousand is logical, marine optics, such as sea light electricity, spectrum day difficult to understand at, Hua Tairuoan, Tianjin Gangdong, Tongfang prestige view,
Sai Mengsi, five bell optics etc..Also there are more patent deposit in some studies in China mechanisms in terms of Raman spectrometer, such as underwater to swash
Light Raman spectrometer (application number: 201710969838.5), Raman spectrometer light path system (application number:
201810012349.5), (application number: 201510925965.6), dual wavelength swashs for a kind of short wavelength laser chirality Raman spectrometer
Light Raman spectrometer (application number: 201720809001.X) etc..
In terms of Raman image, Shanghai Inst. of Technical Physics, Chinese Academy of Sciences declared patent of invention in 2017: a kind of
The adaptive Raman fluorescence imaging combined system of deep space exploration microcell (application number: 201711426271.3), is related to technical field
In terms of being partial to fluorescence imaging；Suo Long-Ji Telin cancer research association was in authorization patent of invention in 2017: wide visual field Raman image
(application number: 201480012550.3), the technical solution of the patent can only obtain one or more monochromes for equipment and correlation technique
Image.In the paper delivered at home, be published within 2017 in " Journal of Agricultural Engineering " " line scan-type Raman EO-1 hyperion at
In a picture technology non-destructive testing milk powder melamine " text, a kind of Raman Specular Highlight spectrum imaging system is proposed, is not directed to
Transmission Raman Hyperspectral imager.In actual application, for specific task, researcher or engineering staff are usual
Think to obtain reflection and transmission Raman high spectrum image simultaneously.Since Raman spectrometer lacks spatial resolution, therefore it cannot permitted
Multi-field inner extensive use.Although Raman hyperspectral imager provides preferable resolving ideas, it can obtain spectrum simultaneously
With the information of Spatial Dimension, to form a three-dimensional Raman spectral image block, but transmission Raman EO-1 hyperion there are no at present
The relevant report of imaging system, and collection reflection and transmission Raman line sweep the open source literature of Hyperspectral imager.
Summary of the invention
The purpose of the present invention is in view of the deficiencies of the prior art and design a kind of Raman high spectrum image acquisition method,
The reflection formed using linear laser and reflective mirror and dichroscope and transmission mode realize that single sweep operation successively captures reflection
With the Raman high spectrum image under transmission both of which, researcher or engineering staff are met significantly and is acquired to using single system
The demand of Raman transmission and Specular Highlight spectrogram picture, can obtain the information of spectrum and Spatial Dimension simultaneously, to form one
Three-dimensional Raman spectral image block, provides preferable resolving ideas for Raman hyperspectral imager, structure is simple, user
Just, convenient, high reliablity is operated, has widened application field, the application study and basic research in particular light spectrum image-forming field mention
For very big benefit and help.
The object of the present invention is achieved like this: a kind of acquisition method of Raman high spectrum image, including image camera, point
The light spectrum image-forming module of light device and camera lens composition, its main feature is that forming Raman using linear laser and reflective mirror and dichroscope
The reflection of EO-1 hyperion and transmission mode, and reflection and transmission Raman EO-1 hyperion are successively carried out to sample to be tested by light spectrum image-forming module
Image Acquisition, successively carry out the Image Acquisition of sample to be tested reflection and transmission Raman EO-1 hyperion, dichroscope setting exists
In optical path between sample stage and light spectrum image-forming module；The reflective-mode of the Raman EO-1 hyperion is that linear laser is arranged two
To Look mirror side, and the image for constituting reflective-mode Raman EO-1 hyperion with angularly disposed dichroscope obtains；The Raman is high
The transmission mode of spectrum be linear laser by three pieces of reflective mirrors reflect in sample stage underface, and with it is horizontally disposed
The image that dichroscope constitutes transmission mode Raman EO-1 hyperion obtains.
There is the present invention single pass to be reflected and be transmitted obtaining for two kinds of Raman high spectrum images compared with prior art
The shortcomings that taking, not only overcoming single-point or multiple spot Raman spectrometer, while a transmission Raman high light spectrum image-forming function is increased,
Support can be provided to probe into the feature of the transmission Raman spectrum of object under test, so as to meet the application need of different scenes
It asks.
Detailed description of the invention
Fig. 1 is the Raman hyper-spectral data gathering schematic diagram of reflective-mode；
Fig. 2 is the Raman hyper-spectral data gathering schematic diagram of transmission mode.
Specific embodiment
The present invention is described in further detail with operating process in particular embodiments below,
Embodiment 1
Refering to attached drawing 1, dichroscope 6 is arranged in the optical path between sample stage 10 and light spectrum image-forming module, linear laser 8
Be arranged in 6 side of dichroscope, and constitute the reflective-mode of linear laser ray with angularly disposed dichroscope 6, and by
As the light spectrum image-forming module that camera 1, optical splitter 2 and camera lens 3 form carries out anti-the sample to be tested 11 being arranged in sample stage 10
The acquisition of Raman high spectrum image is penetrated, the data of acquisition are transmitted to industrial personal computer 4 by mode wirelessly or non-wirelessly and are stored
And processing.
Reflect the optical path of Raman hyper-spectral data gathering mode are as follows: linear laser 8 emits linear laser to dichroscope 6, line
Property laser is reflected on the sample to be tested 11 for being placed on sample stage 10 through dichroscope 6, Raman diffused light via dichroscope 6 into
Enter camera lens 3, Raman diffused light stop to enter with the laser beam of incident light identical frequency after the light splitting of optical splitter 3 spectrum at
As module, and by the imaging of a linear laser illumination line in the completion sample stage 10 of image camera 1, completes target area reflection and draw
The acquisition of graceful EO-1 hyperion.
Refering to attached drawing 2, using three pieces of reflective mirrors 13 by the ray diffraction of linear laser 8 in 10 underface of sample stage, and
The transmission mode of linear laser ray is constituted with horizontally disposed dichroscope 6, and by image camera 1, optical splitter 2 and camera lens 3
The light spectrum image-forming module of composition carries out the acquisition of transmission Raman high spectrum image to the sample to be tested 11 being arranged in sample stage 10,
Its data acquired is transmitted to industrial personal computer 4 by mode wirelessly or non-wirelessly and is stored and handled.
The optical path of transmission Raman hyper-spectral data gathering mode are as follows: linear laser 8 is reflective through three pieces by the linear laser of transmitting
The refraction of mirror 13 is in the underface of sample stage 10, the light through dichroscope 6 cut-off and laser identical frequency, by Raman scattering
Light enters optical splitter 2 by camera lens 3, is imaged on image camera 1 after light splitting.
Above-mentioned sample stage 10 is arranged on the displacement platform 9 equipped with translation mechanism, and is to move horizontally with displacement platform 9；It is described
Dichroscope 6 by 5 linear adjustment laser 8 of stepper motor incident angle；The industrial personal computer 4, stepper motor 5, linear laser
Device 8, displacement platform 9 and light spectrum image-forming module are powered by power supply 7.Above only the present invention is further illustrated, not
To limit this patent, all is equivalence enforcement of the present invention, is intended to be limited solely by within the scope of the claims of this patent.
Claims (1)
1. a kind of acquisition method of Raman high spectrum image, including the light spectrum image-forming being made of image camera, optical splitter and camera lens
Module, it is characterised in that reflection and Transmission Modes using linear laser and reflective mirror and dichroscope composition Raman EO-1 hyperion
Formula, and by light spectrum image-forming module to sample to be tested successively carry out reflection and transmission Raman EO-1 hyperion Image Acquisition, described two to
Look mirror is arranged in the optical path between sample stage and light spectrum image-forming module；The reflective-mode of the Raman EO-1 hyperion is linear laser
Device is arranged in dichroscope side, and the image for constituting reflective-mode Raman EO-1 hyperion with angularly disposed dichroscope obtains；
The transmission mode of the Raman EO-1 hyperion is reflected immediately below sample stage for linear laser by three pieces of reflective mirrors, and with
The image that horizontally disposed dichroscope constitutes transmission mode Raman EO-1 hyperion obtains.
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| CN201910021928.0A CN109557074A (en) | 2019-01-10 | 2019-01-10 | A kind of acquisition method of Raman high spectrum image |
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| CN201910021928.0A CN109557074A (en) | 2019-01-10 | 2019-01-10 | A kind of acquisition method of Raman high spectrum image |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110530843A (en) * | 2019-08-22 | 2019-12-03 | 北京农业智能装备技术研究中心 | The detection method and system of content of starch in a kind of corn kernel |
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2019
- 2019-01-10 CN CN201910021928.0A patent/CN109557074A/en active Pending
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| US5604587A (en) * | 1995-11-16 | 1997-02-18 | World Precision Instruments, Inc. | Long capillary waveguide raman cell |
| CN2591594Y (en) * | 2002-12-31 | 2003-12-10 | 天津市九维光电科技有限公司 | Variable interference filter spectral small size micro-imaging spectrograph |
| CN101019061A (en) * | 2004-06-16 | 2007-08-15 | 比斯泰克半导体系统股份有限公司 | Apparatus and method for inspecting microstructures in reflected or transmitted infrared light |
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| CN110530843A (en) * | 2019-08-22 | 2019-12-03 | 北京农业智能装备技术研究中心 | The detection method and system of content of starch in a kind of corn kernel |
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