CN109535220A - A kind of preparation method of selenium-rich polygonatum kingianurn albumen - Google Patents
A kind of preparation method of selenium-rich polygonatum kingianurn albumen Download PDFInfo
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- CN109535220A CN109535220A CN201811368830.4A CN201811368830A CN109535220A CN 109535220 A CN109535220 A CN 109535220A CN 201811368830 A CN201811368830 A CN 201811368830A CN 109535220 A CN109535220 A CN 109535220A
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- selenium
- rich
- albumen
- rhizoma polygonati
- cloth filterings
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention proposes a kind of preparation method of selenium-rich polygonatum kingianurn albumen, this method, using water extraction and alcohol precipitation method, passes through the techniques such as mashing, extraction, filtering, decoloration, alcohol precipitation using selenium-rich polygonatum kingianurn as raw material, selenium-rich rhizoma polygonati protein content is measured with Kjeldahl's method, obtains high level selenium-rich rhizoma polygonati albumen.It is extracted in the technique by solution of water, using ethyl alcohol as sedimentation agent, on the one hand reduces production cost, on the other hand ensure that the safety of product, it is a kind of green protein production method that when production, which will not pollute the environment,.
Description
Technical field
The present invention relates to a kind of preparation methods of selenium-rich polygonatum kingianurn albumen.
Background technique
Polygonatum kingianurn (Polygonatum kingianum Coll.et Hemsl) Liliaceae Polygonatum herbaceous plant, root shape
The nearly cylindrical or close beaded of stem, tubercle makees irregular water chestnut shape sometimes, plump.Stem top climbs up by holding on to shape.Impeller generator, every wheel 3-10
Piece, bar shaped, strip lanceolar or lanceolar, apex fist volume.Inflorescence tool flower, total bennet is sagging, bennet bract film quality, small, leads to
It is frequently located in bennet lower part；Perianth pink, sliver are 3-5 millimeters long；Filigree is 3-5 millimeters long, Filamentous or two flat-sided, the long 4-6 of anther
Millimeter；Ovary is 4-6 millimeters long.Berry is red, 1-1.5 centimetres of diameter, has 7-12 seed.The month at florescence 3-5, the fruiting period 9-10 month.
Polygonatum kingianurn is common tonic Chinese herbal medicine, has more than 2000 years medication history in China, is Liliaceae (Liliaceae)
Polygonatum (Polygonatum Mill.), its medicinal rhizome.Rhizoma polygonati is mild-natured, sweet in flavor, returns spleen, lung, kidney channel, have tonifying spleen moistening lung,
The effect of supplementing qi and nourishing yin, for treating the diseases such as asthenia, shortness of breath and palpitation, dryness of the lung dry cough, ringworm of the body.In recent years, people are to rhizoma polygonati
And its research of HUANGJING ZANYU CAPSULE deepens continuously, chemical component isolated from rhizoma polygonati plant has alkaloid, flavones, quinone
Class, lignanoid, polysaccharide, steroid saponin and microelement etc..But the rare report of preparation method of polygonatum kingianurn albumen.Polygonatum kingianurn
Albumen has been widely used in functional food.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of preparation methods of selenium-rich polygonatum kingianurn albumen.
A kind of preparation method of selenium-rich polygonatum kingianurn albumen of the present invention, it is characterised in that: the following steps are included:
A. it cleans: cleaning fresh selenium-rich rhizoma polygonati to dustless；
B. it crushes: cleaned selenium-rich rhizoma polygonati is beaten to 300 mesh, and water is added in the ratio that liquid-to-solid ratio is 1.6:1；
C. it extracts: the raw material after proportion is heated into 100min under the conditions of 50 DEG C, it is cooling and with 300 mesh filter-cloth filterings, it presses
The liquid-to-solid ratio of 1.6:1 is extracted 3 times, after combined extract, then at 300 mesh filter-cloth filterings；
D. it decolourizes: carbon column on filtered fluid is decolourized, and with 300 mesh filter-cloth filterings；
E. be concentrated: filtrate is concentrated into 1/5th of total volume at 75 DEG C in above-mentioned steps d, cooling；
F. alcohol precipitation: spilling smart sedimentation with isometric food grade is pure, and with 300 mesh filter-cloth filterings；
G. dry: product is less than 8% in 50 DEG C of vacuum dryings to moisture, packs after being cooled to room temperature.
This method, using water extraction and alcohol precipitation method, passes through mashing, extraction, filtering, decoloration, alcohol precipitation using selenium-rich polygonatum kingianurn as raw material
Etc. techniques, selenium-rich rhizoma polygonati protein content is measured with Kjeldahl's method, obtains high level selenium-rich rhizoma polygonati albumen.In the technique with
Water is solution extraction, using ethyl alcohol as sedimentation agent, on the one hand reduces production cost, on the other hand ensure that the safety of product, raw
It will not be polluted the environment when production, be a kind of green protein production method.
The invention has the benefit that being extracted in the technique by solution of water, using ethyl alcohol as sedimentation agent, reduces and be produced into
This.This method is a kind of green protein production method.Reduce the pollution to environment, ensure that the safety of product.
Specific embodiment
In order to realize invention technological means, reach purpose and effect is easy to understand, below with reference to specific implementation
Mode, the present invention is further explained.
A kind of embodiment 1: preparation method of selenium-rich polygonatum kingianurn albumen of the present invention, comprising the following steps:
A. it cleans: cleaning fresh selenium-rich rhizoma polygonati to dustless；
B. it crushes: cleaned selenium-rich rhizoma polygonati is beaten to 300 mesh, and water is added in the ratio that liquid-to-solid ratio is 1.6:1；
C. it extracts: the raw material after proportion is heated into 100min under the conditions of 50 DEG C, it is cooling and with 300 mesh filter-cloth filterings, it presses
The liquid-to-solid ratio of 1.6:1 is extracted 3 times, after combined extract, then at 300 mesh filter-cloth filterings；
D. it decolourizes: carbon column on filtered fluid is decolourized, and with 300 mesh filter-cloth filterings；
E. be concentrated: filtrate is concentrated into 1/5th of total volume at 75 DEG C in above-mentioned steps d, cooling；
F. alcohol precipitation: spilling smart sedimentation with isometric food grade is pure, and with 300 mesh filter-cloth filterings；
G. dry: product is less than 8% in 50 DEG C of vacuum dryings to moisture, packs after being cooled to room temperature.
Embodiment 2: a kind of preparation method of selenium-rich polygonatum kingianurn albumen of the present invention,
1 cleaning weighs the fresh selenium-rich rhizoma polygonati of 1kg, is cleaned with clean drinking water to dustless, is placed on stainless steel mesh
To without natural water droplet.
Cleaned selenium-rich rhizoma polygonati is beaten to 300 mesh by 2 crushing, and 1.6L water is added.
Raw material after proportion is heated 100min at 50 DEG C by 3 extractions, and cooling, 300 filter clothes are filtered, by 1.4:1's
Liquid-to-solid ratio is extracted 3 times, after combined extract, then at 300 filter-cloth filterings, obtains 5.0L solution
4 decolorations are and primary in 300 filter-cloth filterings by carbon column on 5.0L solution.
The 5 above-mentioned filtrates of concentration are concentrated into total volume 1L at 85 DEG C, cooling.
6 alcohol precipitations have sunk drop with the pure essence of spilling of 1L food grade, and filter.
7 drying products are weighed as 24g, set 20 DEG C of environment preservations in 60 DEG C of vacuum dryings.
Embodiment 3: a kind of preparation method of selenium-rich polygonatum kingianurn albumen of the present invention,
1 cleaning weighs the fresh selenium-rich rhizoma polygonati of 5kg, is cleaned with clean drinking water to dustless, is placed on stainless steel mesh
To without natural water droplet.
Cleaned selenium-rich rhizoma polygonati is beaten to 300 mesh by 2 crushing, and 6.5L water is added.
Raw material after proportion is heated 120min by 3 extractions at 50 DEG C, and cold filtration extracts 3 by the liquid-to-solid ratio of 1.4:1
It is secondary, after combined extract, then at 300 filter-cloth filterings, obtain 36.0L solution
4 decolorations are and primary in 300 filter-cloth filterings by carbon column on 36L solution.
The 5 above-mentioned filtrates of concentration are concentrated into total volume 7.2L at 55 DEG C, cooling.
6 alcohol precipitations have sunk drop with the pure essence of spilling of 7.2L food grade, and filter.
7 drying products are weighed as 126g, set 20 DEG C of environment preservations in 60 DEG C of vacuum dryings.
Embodiment 4: a kind of preparation method of selenium-rich polygonatum kingianurn albumen of the present invention,
1 cleaning weighs the fresh selenium-rich rhizoma polygonati of 10kg, is cleaned with clean drinking water to dustless, is placed on stainless steel mesh
To without natural water droplet.
Cleaned selenium-rich rhizoma polygonati is beaten to 300 mesh by 2 crushing, and 15L water is added.
Raw material after proportion is heated 100min by 3 extractions at 50 DEG C, and cold filtration extracts 3 by the liquid-to-solid ratio of 1.4:1
It is secondary, after combined extract, then at 300 filter-cloth filterings, obtain 54L solution
4 decolorations are and primary in 300 filter-cloth filterings by carbon column on 54L solution.
The 5 above-mentioned filtrates of concentration are concentrated into total volume 11L at 55 DEG C, cooling.
6 alcohol precipitations have sunk drop with the pure essence of spilling of 11L food grade, and filter.
7 drying products are weighed as 259g, set 20 DEG C of environment preservations in 50 DEG C of vacuum dryings.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe originals of the invention
Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle
It is fixed.
Claims (1)
1. a kind of preparation method of selenium-rich polygonatum kingianurn albumen, it is characterised in that: the following steps are included:
A. it cleans: cleaning fresh selenium-rich rhizoma polygonati to dustless；
B. it crushes: cleaned selenium-rich rhizoma polygonati is beaten to 300 mesh, and water is added in the ratio that liquid-to-solid ratio is 1.6:1；
C. it extracts: the raw material after proportion is heated into 100min under the conditions of 50 DEG C, it is cooling and with 300 mesh filter-cloth filterings, by 1.6:1
Liquid-to-solid ratio extract 3 times, after combined extract, then at 300 mesh filter-cloth filterings；
D. it decolourizes: carbon column on filtered fluid is decolourized, and with 300 mesh filter-cloth filterings；
E. be concentrated: filtrate is concentrated into 1/5th of total volume at 75 DEG C in above-mentioned steps d, cooling；
F. alcohol precipitation: spilling smart sedimentation with isometric food grade is pure, and with 300 mesh filter-cloth filterings；
G. dry: product is less than 8% in 50 DEG C of vacuum dryings to moisture, packs after being cooled to room temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811368830.4A CN109535220A (en) | 2018-11-16 | 2018-11-16 | A kind of preparation method of selenium-rich polygonatum kingianurn albumen |
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| CN201811368830.4A CN109535220A (en) | 2018-11-16 | 2018-11-16 | A kind of preparation method of selenium-rich polygonatum kingianurn albumen |
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| CN109535220A true CN109535220A (en) | 2019-03-29 |
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| CN201811368830.4A Pending CN109535220A (en) | 2018-11-16 | 2018-11-16 | A kind of preparation method of selenium-rich polygonatum kingianurn albumen |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116035153A (en) * | 2023-03-06 | 2023-05-02 | 安徽中志科技有限公司 | Selenium-enriched collagen dietary fiber protein powder solid beverage |
Citations (6)
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| US20070027078A1 (en) * | 2005-07-26 | 2007-02-01 | The Chinese University Of Hong Kong | Isolated proteins from a traditional Chinese medicine Yuzhu and use thereof |
| CN101984839A (en) * | 2010-10-18 | 2011-03-16 | 湖北省农科院中药材研究所 | Method for preparing pueraria lobata protein |
| CN105054043A (en) * | 2015-09-15 | 2015-11-18 | 利川硒草生物科技有限公司 | Blood glucose-reducing and blood pressure-reducing selenium-rich food and preparation method thereof |
| CN105821105A (en) * | 2016-03-30 | 2016-08-03 | 蔡庭守 | Process for extracting rhizoma polygonati entity small molecular peptide |
| CN106083990A (en) * | 2016-06-02 | 2016-11-09 | 安徽新泰药业有限公司 | A kind of sealwort peptide extracting method and sealwort complex peptides powder |
| CN106852498A (en) * | 2015-12-09 | 2017-06-16 | 池州千年坊生物科技有限公司 | A kind of sealwort albumen powder and preparation method thereof |
-
2018
- 2018-11-16 CN CN201811368830.4A patent/CN109535220A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070027078A1 (en) * | 2005-07-26 | 2007-02-01 | The Chinese University Of Hong Kong | Isolated proteins from a traditional Chinese medicine Yuzhu and use thereof |
| CN101984839A (en) * | 2010-10-18 | 2011-03-16 | 湖北省农科院中药材研究所 | Method for preparing pueraria lobata protein |
| CN105054043A (en) * | 2015-09-15 | 2015-11-18 | 利川硒草生物科技有限公司 | Blood glucose-reducing and blood pressure-reducing selenium-rich food and preparation method thereof |
| CN106852498A (en) * | 2015-12-09 | 2017-06-16 | 池州千年坊生物科技有限公司 | A kind of sealwort albumen powder and preparation method thereof |
| CN105821105A (en) * | 2016-03-30 | 2016-08-03 | 蔡庭守 | Process for extracting rhizoma polygonati entity small molecular peptide |
| CN106083990A (en) * | 2016-06-02 | 2016-11-09 | 安徽新泰药业有限公司 | A kind of sealwort peptide extracting method and sealwort complex peptides powder |
Non-Patent Citations (2)
| Title |
|---|
| QUNGUANG JIANG ET AL.: ""Extraction and bioactivity of polygonatum polysaccharides"", 《INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES》 * |
| 何庆玲 等: ""多花黄精总蛋白质提取工艺优化"", 《资源开发与市场》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116035153A (en) * | 2023-03-06 | 2023-05-02 | 安徽中志科技有限公司 | Selenium-enriched collagen dietary fiber protein powder solid beverage |
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Application publication date: 20190329 |