CN109528932A - A kind of processing method of probiotics stone flower - Google Patents
A kind of processing method of probiotics stone flower Download PDFInfo
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Abstract
The invention discloses a kind of processing methods of probiotics dendrobium nobile flower prod.The product is, with Ganoderma Lucidum, lactic acid bacteria fermenting agent, to be prepared using fresh stone flower as raw material with two sections of zymotechniques.Preparation method is related to fresh stone flower pretreatment, fermentation substrate allotment, Ganoderma Lucidum aerobic fermentation, lactobacillus acidophilus and Bifidobacterium anaerobic fermentation, fermentation material drying.Compared with prior art, this method is using fresh dendrobium nobile as unique raw material, using functional probiotics fermention, polysaccharide dissolution rate is obviously improved in manufactured product, in addition to the nutritional ingredient for remaining stone flower itself, also containing functional probiotics, a variety of zymoproteins, there are the plurality of health care functions such as anti-aging, antibacterial anti-inflammatory, enhancing immunity of organism.
Description
Technical field
The present invention relates to stone flower manufacture field, in particular to a kind of processing method of probiotics stone flower.
Background technique
Dendrobium nobile is Chinese tradition rare Chinese medicine, can be divided into the dozens ofs such as iron sheet, Jin Chai, copper sheet, Huoshan by form, place of production etc.
Kind.The main active contained in stone flower is that dendrobium polysaccharide, essential oil, dendrobine, amino acid and its derivative have increasing
The composite reactives function such as strong immune, antifatigue, anti-oxidant, blood pressure lowering, hypoglycemic, antitumor.
Document (Zhao Shiguang, Wang Shiran, Xue Zhenglian, et al. low energy ion beam implantation mutagenic and breeding laccase high yield bacterial strain [J]
Laser biology journal, 2009,18 (4): 534-538.) in using utilize low energy N+Beam injection technique is to laccase producing strains Ganoderma Lucidum
(Ganoderma lucidum) U60 mycelium carries out irradiation mutagenesis, a series of UIM mutant strains is obtained, including UIM-281
It is laccase high yield bacterial strain with UIM-135 mutant strain, metabolite laccase has extensive Substratspezifitaet, can aoxidize more
Kind phenols, aromatic amine compounds, steroid hormone, biochrome and metallo-organic compound, at papermaking, food processing, waste water
Reason, soil sanitation, dye decolored, biosensor, immune detection etc. are with a wide range of applications.
Currently, be mainly to brew the jasmine tea of form using stone flower as the product of Raw material processing, mostly with tealeaves, Radix Glycyrrhizae, arhat
The cooperation of the plants such as fruit is process.The type product is related to withering using the common processes and technology of tea processing process
It the links such as withers, finish, rubbing, drying.The probiotics of particular functionality is not contained in the type product.
Currently, to be related to following two field in stone flower and its processing technology:
1, stone flower dry product
The multinomial technical essential in the field at present are as follows: single to use fresh stone flower for raw material, cleaned, salt marsh, after drying,
It is dried or is suppressed, dry product stone flower is made.
2, dendrobium nobile flower tea
Feature is that stone flower is compounded with tealeaves, processes dendrobium nobile flower tea.Technically use a system of tea processing
Column technique, be related to fresh flower wither, finish, rubbing, scenting, pile fermentation, the technologies such as baking.Products obtained therefrom is dendrobium nobile flower tea, without benefit
Raw bacterium ingredient.
Chinese Patent Application No.: 201610874580.6 disclose a kind of golden flower dendrobium tea and preparation method thereof, preparation side
Method are as follows: after being withered by dendrobium candidum flower, rub processing, carry out the wet fermentation of heap；Treated dendrobium candidum flower is turned over
It fries；Then boiling water is poured into in spending through processed dendrobium candidum, then completely cuts off air, ferments；Then golden flower is added, passes through
Charcoal roasting is carried out after mixing；The ageing of processed mixture is placed 100 days~180 days to get made golden flower dendrobium tea.The present invention
By the tea making in conjunction with golden flower of dendrobium candidum flower, the nutritive value of dendrobium candidum is taken full advantage of, in conjunction with golden flower, is even more improved
The mouthfeel and condition of golden flower dendrobium tea, so that golden flower dendrobium tea obtained has good economic value and the market competitiveness.
Chinese Patent Application No.: 201710732032.4 disclose a kind of processing technology of HERBA DENDROBII jasmine tea, be by
HERBA DENDROBII flower is cleaned with clear water, is then withered, and the HERBA DENDROBII that will wither that treated, which is spent, first once to be finished, then
Once rubbed, then carry out secondary water-removing, carried out after secondary water-removing it is secondary rub, then carry out HERBA DENDROBII flower and pierce
Pears piece, green tea dry tea mixed fermentation processing, by drying materials after fermentation process and Titian to get.The present invention is by by golden hairpin stone
Dry measure used in former times flower, Rosa roxburghii Tratt slice and green tea dry tea carry out comprehensive allotment, can not only enrich the benefit materials type and content in tea, and
The mouthfeel of single tea can also be improved.
Chinese Patent Application No.: 201610476124.6, disclose a kind of dendrobium candidum premium tea compressed tea and its preparation
Method.The dendrobium candidum premium tea compressed tea is 30~50% dendrobium candidum fresh flowers and 50~70% black tea tea by mass ratio
Blueness withers by picking and again, rubs, rubbing and cut, ferment, dry, Titian, press shaping and be prepared.Stone made of the present invention
Dry measure used in former times premium tea compressed tea mellow in taste it is fresh it is refreshing, fragrant and sweet the fragrance of flowers is strong, helpful, shape is graceful, it is easy to carry, be easy to brew,
Evaded the serious burnt fiery taste of made tea, and the natural fragrance of a flower and it is fragrant and sweet it is not high not persistently, the deficiencies of easily band is bitter and not mature, while most
Big degree remains the material composition of polysaccharide contained by tealeaves and stone flower and two kinds of polyphenol most healthcare function.
Though have certain health-care efficacy by the product that stone flower preparation method disclosed in above-mentioned patent obtains, its
It is clearly present following disadvantage:
1, stone flower product function is single.
Stone flower dry product is simple cleaning in the prior art, drying technology is process, and is stone flower in physical aspect
On secondary operation, only have the trophic function value of stone flower itself, and because of cell-free broken wall, effective component is molten when Instant Drinks
It is ineffective out.
2, dendrobium nobile flower tea raw material complicated composition can not embody the main functionality of stone flower.
Its definition of existing dendrobium nobile flower tea product is the compound compatibility product of Hua Hecha, and main body is tea rather than stone flower,
The type product is different from the positioning of this technology product.
3, probiotics is free of in stone flower or dendrobium nobile flower tea, healthcare function is single.
The pile-fermentation of stone flower it has been related in the prior art, but this fermentation method is spontaneous fermentation plus air oxygen
The collective effect of change is as a result, what spontaneous fermentation therein utilized is environmental microorganism, and in later period stoving process, microorganism quilt
Heat sterilization no longer contains microorganism and its metabolite in product.
Summary of the invention
The technical problems to be solved by the invention: dendrobium nobile flower prod is using the methods of drying, compounding to fresh dendrobium nobile at present
Flower carry out the reprocessing of secondary physical form, for keep stone flower finished product form it is complete, broken wall can not be carried out to it, spend in battalion
Feeding substance can not be released effectively, and finished product stone flower healthcare function is single, the natural nutrient component only containing stone flower itself.Needle
To problems and disadvantage present in current dendrobium nobile flower prod and preparation process, the present invention provides a kind of probiotics stone flower
Processing method, to overcome the above problem.
In order to solve the above technical problems, the present invention provides technical solution below:
A kind of processing method of probiotics stone flower includes following specific steps:
(1) fresh stone flower pretreatment: manually harvesting fresh stone flower, after sorting removal of impurities, the impurity such as clear water rinsed surfaces floating dust,
It under the conditions of 40 DEG C~50 DEG C, was dried through 4-6 hours, after removing surface moisture, 4 DEG C of -10 DEG C of refrigerations are spare；
(2) fermentation substrate is deployed: being taken pretreated fresh stone flower, with atomizers spray clear water to flower surface, is reached wet
Moisten and hold agglomerating degree of not dripping, the moist heat sterilization 5min-10min at 110 DEG C~115 DEG C, being packed into 500-1000mL can take out
Fermentation flask is filtered, is cooled to room temperature, leavening to be accessed；
(3) prepared by leavening: Ganoderma Lucidum UIM-135 is accessed into potato dextrose broth, at 28 DEG C~30 DEG C,
Mycelium pellet is collected as leavening A in shaken cultivation 3~4 days under conditions of 150~180r/min；With milk containing 3%-5%, 4~
The MS culture medium of 8% oligofructose carries out Liquid static culture to lactobacillus acidophilus and Bifidobacterium at 30 DEG C~35 DEG C, takes
The bacterium solution of culture in 15~20 hours is as leavening B；
(4) stone flower is fermented: stone flower zymotechnique uses two sections of fermentation methods, and leading portion fermentation is carried out using leavening A
Aerobe fermentation, back segment fermentation carry out anaerobic fermentation using leavening B；
(5) probiotics stone flower is dry: the stone flower fermentation material after taking back segment to ferment 5-15 days, paves on pallet, thickness
After degree 0.5-1.0cm, -25 DEG C of pre-freeze 5h, 18h is freeze-dried under the conditions of -40 DEG C, gained finished product is sub-packed in vacuum seal bag,
Obtain the probiotics dendrobium nobile flower prod.
Preferably, the fresh stone flower is the fresh stone flower in the petal phase, and petal is not yet unfolded, is wrapped in petal,
1~4h is picked before blooming, and is pre-processed in 0.5~2h after picking.
Preferably, the fresh stone flower be derived from dendrobium candidum, HERBA DENDROBII, dendrobium hancockii Rolfe, weight lip dendrobium nobile, the red dendrobium nobile of larynx,
Long Soviet Union's dendrobium nobile, Dendrobium thyrsiflorum, wing stalk dendrobium nobile, purple dendrobium, Dendrobium Moniliforme, Dendrobium chrysanthum, Dendrobium Chrysotoxum Lindl, yellow larynx dendrobium nobile, a beading
One of dendrobium nobile is a variety of.
Preferably, leading portion fermentation the specific steps are after having been charged into pretreatment stone flower filter in fermentation flask by
10%~15% inoculum concentration accesses UIM-135 mycelium pellet, after gently stirring evenly, is placed in 28 DEG C~30 DEG C ferment at constant temperature, every Nikkei hair
The pressure of ferment bottle suction filtration mouth is passed through filtrated air 1~3 time, to maintain aerobic condition；After continuing fermentation 5-15 days, naked eyes between stone flower
It can be seen that after UIM-135 mycelia generates, leading portion fermentation ends.
Preferably, back segment fermentation the specific steps are in leading portion fermentation period terminal, continuation connects with 5%~10%
Kind amount, sprinkling access leavening B, shakes up, and after fermented bottle suction filtration mouth extraction air, 30 DEG C under confined conditions~40 DEG C anaerobism are sent out
Ferment 5~15 days；Stirring 2~3 times daily in first 5 days, stir 1~5 minute every time, are not stirred for later, standing for fermentation is to terminal.
Preferably, the total concentration of thallus is 5 × 10 in the leavening B4~5CFU/mL, lactobacillus acidophilus and Bifidobacterium
The ratio between viable count be 1~10:1.
A kind of probiotics stone flower of above-mentioned processing method preparation, is 8.5 × 10 comprising the prebiotic bacterium number of living body4~6CFU/g,
Total starches content is 90~131mg/mL, and total amino acid content is 1125~1455 μ g/mL, and total phenol content is 170~185mg/
L, pH value 3.74~3.87.
It preferably, also include four kinds of functional enzymes, respectively superoxide dismutase, protease, shallow lake in probiotics stone flower
Powder enzyme and lipase.
Preferably, the superoxide dismutase enzyme activity is 6500~8500U/L；The enzyme activity of protease be 1840.8~
2490.4U/L；Amylase enzyme activity is 325~334.6U/L；Lipase activity is 100~450U/L.
It is that the present invention obtains the utility model has the advantages that
1, contain functional probiotics and its metabolite in dendrobium nobile flower prod, remove the battalion for remaining stone flower itself in product
It forms exceptionally, also comprising multi-nutrients such as probiotics, dendrobium polysaccharide, protease, lipase, amylase, has and remove freely
Base, anti-aging, antibacterial anti-inflammatory, enhancing immunity of organism, the plurality of health care functions such as thin body of losing weight.
2, probiotics decomposes stone flower microstructure, promotes the dissolution of polysaccharide component, and growth of probiotics, proliferation function are realized
The decomposition of stone flower institutional framework, promotes the release of stone flower polysaccharide, polysaccharide release rate improves 52.2%.
3, dry using freeze-drying mode, volatile essential oil ingredient and the probiotics of stone flower are utmostly remained, it is prebiotic
Bacterium living body bacterium number can reach 8.5 × 106CFU/g。
4, probiotics dendrobium nobile flower prod antioxidant activity is obviously improved, and zymoprotein vigor significantly improves.
Detailed description of the invention
Fig. 1: probiotics stone flower preparation process flow.
Fig. 2: thalli growth curve in leavening B preparation process.
Fig. 3: discomposing effect of the leavening to stone flower internal structure.
Fig. 4: probiotics stone flower fermentation process hydroxyl radical free radical clearance rate dynamic change.
Fig. 5: probiotics stone flower fermentation process DPPH free radical scavenging activity dynamic change.
Fig. 6: probiotics stone flower fermentation process ABTS free radical scavenging activity dynamic change.
Fig. 7: probiotics stone flower fermentation process total phenol content dynamic change.
Fig. 8: superoxide dismutase enzyme activity changes in fermentation process.
Fig. 9: proteinase activity changes in fermentation process.
Figure 10: amylase enzyme activity changes in fermentation process.
Figure 11: lipase activity changes in fermentation process.
Figure 12: total starches changes of contents in fermentation process.
Figure 13: total amino acid changes of contents in fermentation process.
Figure 14: PH changes in fermentation process.
Specific embodiment
Below by the description to embodiment, specific embodiments of the present invention will be described in further detail, with side
Those skilled in the art is helped to have more complete, accurate and deep understanding to inventive concept of the invention, technical solution.
Embodiment 1: it is prepared as follows probiotics stone flower:
(1) fresh stone flower pretreatment: dendrobium species select dendrobium candidum, and artificial harvesting is in the fresh stone flower of petal phase
(petal is not yet unfolded, and is wrapped in petal), 1h is picked before blooming, and is pre-processed in 0.5h after picking, after sorting removal of impurities,
The impurity such as clear water rinsed surfaces floating dust were dried under the conditions of 40 DEG C through 4 hours, and after removing surface moisture, 4 DEG C of refrigerations are spare；
(2) fermentation substrate is deployed: being taken pretreated fresh stone flower, with atomizers spray clear water to flower surface, is reached wet
Moisten and hold agglomerating degree of not dripping, the moist heat sterilization 5min at 110 DEG C, fermentation flask can be filtered by being packed into 500mL, be cooled to room
Temperature, leavening to be accessed；
(3) prepared by leavening: Ganoderma Lucidum UIM-135 is accessed potato dextrose broth, at 28 DEG C, 150r/
Mycelium pellet is collected as leavening A in shaken cultivation 3 days under conditions of min；With milk containing 3wt%, the MS of 4wt% oligofructose
Culture medium carries out Liquid static culture to lactobacillus acidophilus and Bifidobacterium at 30 DEG C, takes the bacterium solution of culture in 15 hours as hair
The total concentration of thallus is 5 × 10 in ferment agent B, leavening B4CFU/mL, wherein the viable count of lactobacillus acidophilus and Bifidobacterium it
Than for 1:1；
(4) stone flower is fermented: stone flower zymotechnique uses two sections of fermentation methods, and leading portion fermentation is UIM-135 aerobic fermentation,
Back segment is lactobacillus acidophilus and Bifidobacterium anaerobic fermentation；
Leading portion fermentation: filtering in fermentation flask for stone flower accesses UIM- by 10wt% inoculum concentration after having been charged into pretreatment
135 mycelium pellets after gently stirring evenly, are placed in 28 DEG C of DEG C of ferment at constant temperature, and daily fermented bottle suction filtration mouth pressure is passed through filtrated air 1
It is secondary, to maintain aerobic condition；After continuing fermentation 5 days, after the visible UIM-135 mycelia of naked eyes generates between stone flower, leading portion fermentation knot
Beam；
Back segment fermentation: in leading portion fermentation period terminal, continuing with 5wt% inoculum concentration, and sprinkling access leavening B shakes up,
After fermented bottle suction filtration mouth extraction air, 30 DEG C anaerobic fermentation 5 days under confined conditions；Stirring 2 times daily in first 5 days, stir 1 every time
Minute, it is not stirred for later, standing for fermentation is to terminal.
(5) probiotics stone flower is dry: the stone flower fermentation material after taking fermentation 5 days, paves on pallet, thickness
After 0.5cm, -25 DEG C of pre-freeze 5h, 18h is freeze-dried under the conditions of -40 DEG C, gained finished product is sub-packed in vacuum seal bag to get arriving
The probiotics dendrobium nobile flower prod.
Measured after bacterium colony culture counting the prebiotic bacterium number of living body in probiotics stone flower (lactobacillus acidophilus and Bifidobacterium it
With) it is 8.5 × 104CFU/g。
Embodiment 2: it is prepared as follows probiotics stone flower:
(1) fresh stone flower pretreatment: dendrobium species select dendrobium candidum, HERBA DENDROBII, dendrobium hancockii Rolfe, stone flower quantity ratio
Example is 1:3:1, and artificial harvesting is in the fresh stone flower (petal is not yet unfolded, and is wrapped in petal) of petal phase, the 4h before blooming
Picking, and pre-processed in 2h after picking, after sorting removal of impurities, the impurity such as clear water rinsed surfaces floating dust, under the conditions of 50 DEG C, through 6
Hour drying, after removing surface moisture, 10 DEG C of refrigerations are spare；
(2) fermentation substrate is deployed: being taken pretreated fresh stone flower, with atomizers spray clear water to flower surface, is reached wet
Moisten and hold agglomerating degree of not dripping, the moist heat sterilization 10min at 115 DEG C, fermentation flask can be filtered by being packed into 1000mL, be cooled to room
Temperature, leavening to be accessed；
(3) prepared by leavening: Ganoderma Lucidum UIM-135 is accessed potato dextrose broth, at 30 DEG C, 180r/
Mycelium pellet is collected as leavening A in shaken cultivation 4 days under conditions of min；With milk containing 5wt%, the MS of 8wt% oligofructose
Culture medium carries out Liquid static culture to lactobacillus acidophilus and Bifidobacterium at 35 DEG C, takes the bacterium solution of culture in 20 hours as hair
The total concentration of thallus is 5 × 10 in ferment agent B, leavening B5CFU/mL, wherein the viable count of lactobacillus acidophilus and Bifidobacterium it
Than for 10:1；
(4) stone flower is fermented: stone flower zymotechnique uses two sections of fermentation methods, and leading portion fermentation is UIM-135 aerobic fermentation,
Back segment is lactobacillus acidophilus and Bifidobacterium anaerobic fermentation；
Leading portion fermentation: filtering in fermentation flask for stone flower accesses UIM- by 15wt% inoculum concentration after having been charged into pretreatment
135 mycelium pellets after gently stirring evenly, are placed in 30 DEG C of ferment at constant temperature, and daily fermented bottle suction filtration mouth pressure is passed through filtrated air 3 times,
To maintain aerobic condition；After continuing fermentation 15 days, after the visible UIM-135 mycelia of naked eyes generates between stone flower, leading portion fermentation ends；
Back segment fermentation: in leading portion fermentation period terminal, continue with 10wt% inoculum concentration, sprinkling access leavening B shakes
It is even, after fermented bottle suction filtration mouth extraction air, 40 DEG C anaerobic fermentation 15 days under confined conditions；Stirring 3 times daily in first 5 days, every time
Stirring 5 minutes, is not stirred for later, and standing for fermentation is to terminal.
(5) probiotics stone flower is dry: the stone flower fermentation material after taking fermentation 15 days, paves on pallet, thickness
After 1.0cm, -25 DEG C of pre-freeze 5h, 18h is freeze-dried under the conditions of -40 DEG C, gained finished product is sub-packed in vacuum seal bag to get arriving
The probiotics dendrobium nobile flower prod.
Measured after bacterium colony culture counting the prebiotic bacterium number of living body in probiotics stone flower (lactobacillus acidophilus and Bifidobacterium it
With) it is 8.5 × 106CFU/g。
Embodiment 3: it is prepared as follows probiotics stone flower:
(1) fresh stone flower pretreatment: dendrobium species select weight lip dendrobium nobile, the red dendrobium nobile of larynx, long Soviet Union's dendrobium nobile, and artificial harvesting is in
The fresh stone flower (petal is not yet unfolded, and is wrapped in petal) of petal phase, 2h is picked before blooming, and is located in advance in 1h after picking
Reason, after sorting removal of impurities, the impurity such as clear water rinsed surfaces floating dust were dried under the conditions of 45 DEG C through 5 hours, after removing surface moisture,
7 DEG C of refrigerations are spare；
(2) fermentation substrate is deployed: being taken pretreated fresh stone flower, with atomizers spray clear water to flower surface, is reached wet
Moisten and hold agglomerating degree of not dripping, the moist heat sterilization 8min at 115 DEG C, fermentation flask can be filtered by being packed into 750mL, be cooled to room
Temperature, leavening to be accessed；
(3) prepared by leavening: Ganoderma Lucidum UIM-135 is accessed potato dextrose broth, at 29 DEG C, 160r/
Mycelium pellet is collected as leavening A in shaken cultivation 3 days under conditions of min；With the MS culture containing 4% milk, 6% oligofructose
Base carries out Liquid static culture to lactobacillus acidophilus and Bifidobacterium at 33 DEG C, takes the bacterium solution of culture in 17 hours as leavening
The total concentration of thallus is 1 × 10 in B, leavening B5CFU/mL, wherein the ratio between lactobacillus acidophilus and the viable count of Bifidobacterium are
5:1；
(4) stone flower is fermented: stone flower zymotechnique uses two sections of fermentation methods, and leading portion fermentation is UIM-135 aerobic fermentation,
Back segment is lactobacillus acidophilus and Bifidobacterium anaerobic fermentation；
Leading portion fermentation: filtering in fermentation flask for stone flower accesses UIM- by 13wt% inoculum concentration after having been charged into pretreatment
135 mycelium pellets after gently stirring evenly, are placed in 28 DEG C of ferment at constant temperature, and daily fermented bottle suction filtration mouth pressure is passed through filtrated air 2 times,
To maintain aerobic condition；After continuing fermentation 10 days, after the visible UIM-135 mycelia of naked eyes generates between stone flower, leading portion fermentation ends；
Back segment fermentation: in leading portion fermentation period terminal, continuing with 7wt% inoculum concentration, and sprinkling access leavening B shakes up,
After fermented bottle suction filtration mouth extraction air, 35 DEG C anaerobic fermentation 10 days under confined conditions；Stirring 3 times daily in first 3 days, are stirred every time
It 3 minutes, is not stirred for later, standing for fermentation is to terminal.
(5) probiotics stone flower is dry: the stone flower fermentation material after taking fermentation 10 days, paves on pallet, thickness
After 0.7cm, -25 DEG C of pre-freeze 5h, 18h is freeze-dried under the conditions of -40 DEG C, gained finished product is sub-packed in vacuum seal bag to get arriving
The probiotics dendrobium nobile flower prod.
Measured after bacterium colony culture counting the prebiotic bacterium number of living body in probiotics stone flower (lactobacillus acidophilus and Bifidobacterium it
With) it is 8 × 105CFU/g。
Embodiment 4: it is prepared as follows probiotics stone flower:
(1) fresh stone flower pretreatment: dendrobium species select Dendrobium thyrsiflorum, wing to obstruct dendrobium nobile, purple dendrobium, Dendrobium Moniliforme, no
Flower number ratio with dendrobium species is arbitrary value, and (petal is not yet unfolded fresh stone flower of the artificial harvesting in the petal phase, package
In in petal), 3h is picked before blooming, and is pre-processed in 1.5h after picking, after sorting removal of impurities, clear water rinsed surfaces floating dust etc.
Impurity was dried under the conditions of 45 DEG C through 4 hours, and after removing surface moisture, 6 DEG C of refrigerations are spare；
(2) fermentation substrate is deployed: being taken pretreated fresh stone flower, with atomizers spray clear water to flower surface, is reached wet
Moisten and hold agglomerating degree of not dripping, the moist heat sterilization 5min at 115 DEG C, fermentation flask can be filtered by being packed into 1000mL, be cooled to room
Temperature, leavening to be accessed；
(3) prepared by leavening: Ganoderma Lucidum UIM-135 is accessed potato dextrose broth, at 28 DEG C, 150r/
Mycelium pellet is collected as leavening A in shaken cultivation 4 days under conditions of min；With the MS culture containing 5% milk, 7% oligofructose
Base carries out Liquid static culture to lactobacillus acidophilus and Bifidobacterium at 31 DEG C, takes the bacterium solution of culture in 16 hours as leavening
The total concentration of thallus is 5 × 10 in B, leavening B5CFU/mL, wherein the ratio between lactobacillus acidophilus and the viable count of Bifidobacterium are
8:1；
(4) stone flower is fermented: stone flower zymotechnique uses two sections of fermentation methods, and leading portion fermentation is UIM-135 aerobic fermentation,
Back segment is lactobacillus acidophilus and Bifidobacterium anaerobic fermentation；
Leading portion fermentation: filtering in fermentation flask for stone flower accesses UIM- by 11wt% inoculum concentration after having been charged into pretreatment
135 mycelium pellets after gently stirring evenly, are placed in 30 DEG C of ferment at constant temperature, and daily fermented bottle suction filtration mouth pressure is passed through filtrated air 3 times,
To maintain aerobic condition；After continuing fermentation 14 days, after the visible UIM-135 mycelia of naked eyes generates between stone flower, leading portion fermentation ends；
Back segment fermentation: in leading portion fermentation period terminal, continuing with 6wt% inoculum concentration, and sprinkling access leavening B shakes up,
After fermented bottle suction filtration mouth extraction air, 34 DEG C anaerobic fermentation 11 days under confined conditions；Stirring 2 times daily in first 4 days, are stirred every time
It 4 minutes, is not stirred for later, standing for fermentation is to terminal.
(5) probiotics stone flower is dry: the stone flower fermentation material after taking fermentation 14 days, paves on pallet, thickness
After 0.9cm, -25 DEG C of pre-freeze 5h, it is freeze-dried under the conditions of -40 DEG C for 24 hours, gained finished product is sub-packed in vacuum seal bag to get arriving
The probiotics dendrobium nobile flower prod.
Measured after bacterium colony culture counting the prebiotic bacterium number of living body in probiotics stone flower (lactobacillus acidophilus and Bifidobacterium it
With) it is 9 × 106CFU/g。
Embodiment 5: the present embodiment and embodiment 3 the difference is that dendrobium species select Dendrobium chrysanthum, Dendrobium Chrysotoxum Lindl,
Yellow larynx dendrobium nobile, Falconer Dendrobium, the flower number ratio of different dendrobium species are 1:5:2:3.
Measured after bacterium colony culture counting the prebiotic bacterium number of living body in probiotics stone flower (lactobacillus acidophilus and Bifidobacterium it
With) it is 7.5 × 106CFU/g。
For the every physical and chemical index for measuring probiotics stone flower prepared by the present invention, following functional assessment experiment is carried out:
Experiment is all made of the probiotics stone flower prepared in embodiment 3 as test sample below.
1, thalli growth curve in leavening B preparation process.
In the culture medium containing milk and oligofructose after middle inoculating lactobacillus acidophilus and bifidobacterium species, 30 degrees Celsius
Stationary culture, and a bacterial concentration is every two hours measured, using the OD value of bacterium solution under spectrophotometric determination 440nm wavelength,
And time-history curves, as thalli growth curve (Fig. 2) are made according to the time, according to thalli growth characteristic to determine that subsequent fermentation is raw
The termination time point of anaerobic fermentation during production.
2, discomposing effect of the leavening to stone flower internal structure.
Electron-microscope scanning is carried out after the stone flower longitudal section epidermal tissue of fermentation front and back is used specimens paraffin embedding slices, and is taken pictures
It compares (Fig. 3 A and Fig. 3 B).Fig. 3 A is the dendrobium nobile flower piece longitudal section epidermal tissue before fermentation, and compact texture, hole is fewer, figure
3B is the dendrobium nobile flower piece longitudal section epidermal tissue after fermentation, and quality is obviously loose, and the hole between tissue has to a certain degree
Increase, this shows that the growth of lactic acid bacteria causes to decompose to the internal structure of stone flower tissue, is conducive to the nutriments such as polysaccharide
Exudation, release.
3, the clearance rate of hydroxyl radical free radical, DPPH free radical, ABTS free radical in fermentation process is measured.
According to document: Gao Shihua, Ye Gaojie, Qin Jiangke wait the Study on Its Antioxidant Activity in Vitro of oil tea polysaccharide and polyphenol
[J] food industry, 2013 (11): provided in 163-166. method measurement fermentation process in probiotics stone flower to hydroxyl from
By the clearance rate of base, DPPH free radical, ABTS free radical.As a result such as Fig. 4, Fig. 5, Fig. 6.
4, the total phenol content in fermentation process in probiotics fermention stone flower is measured.
According to document: Li Da, Ma Congyu, Lv Qinglin wait the measurement of different sources skimmed milk wood kernel total phenol content and its life
Object activity [J] China Medicine University journal, the determining total phenol method that 2018 (5) are provided measure probiotics hair in fermentation process
Total phenol content in ferment stone flower, as a result as shown in Figure 7.
5, four kinds of function enzyme activity measurement results and analysis
5.1 superoxide dismutases (SOD enzyme) enzyme activity compares
As seen from Figure 8,8 days before fermentation, SOD enzyme enzyme activity is risen rapidly in experimental group fermentation stone flower, is reached most
Big value 8600U/L after short time enzyme activity reduces, and starts to continue rising, and experimental group SOD enzyme enzyme activity is higher than blank after fermentation in 30 days
Group 11.6%.
5.2 proteinase activities compare
As seen from Figure 9, in entire fermentation process, the whole enzyme activity of experimental group is all above blank control group, real
Testing group is respectively 1840.8U/mL and 2490.4U/mL, reality after fermentation in 30 days respectively in two peak values of appearance in the 8th day and the 24th day
It tests group and increases 62.68% than blank control group.
5.3 amylase enzyme activity compare
As seen from Figure 10, the amylase enzyme activity of experimental group and blank group does not occur too greatly in overall fermentation process
Difference, experimental group enzyme activity reached up to 334.6U/L at 24 days, the enzyme activity of experimental group and blank control group after fermentation in 30 days
It is almost consistent, illustrate that fermentation stone flower does not reduce the reduction that stone flower itself includes amylase activity because of the intervention of microorganism.
5.4 lipase activities compare
As seen from Figure 11, in 30 days fermentation process, experimental group lipase activity was all significantly high at first 20 days
In blank control group, the lipase activity of experimental group reaches peak 450U/L on day 4, and the lipase enzyme of blank control group
Living and being constantly in relatively low level is up to 55U/L.Illustrate ability of the stone flower with significant elimination fat of fermenting, it is reachable
To certain antiobesity action.
6, the measurement result and analysis of total reducing sugar, total amino acid in stone flower of fermenting
The comparison of 6.1 total starches contents
As seen from Figure 12, experimental group and blank control group total sugar content variation tendency maintain consistency, are all total
The trend risen is presented in body, this is because containing a large amount of dendrobium polysaccharide in dendrobium nobile fresh flower, as the variation of fermentation time can not
It is disconnected to have dendrobium polysaccharide in juice enters entire fermentation system.Simultaneously as it can be seen that before earlier fermentation the 8th day, in experimental group
Polyoses content is apparently higher than control group, has been reached and has released because the growth of microorganism has decomposed the institutional framework of stone flower
Put the effect for including polysaccharide.Later experiments group polyoses contents is lower than concurrent control group, is the metabolic exhaustion portion because of microorganism
Divide polysaccharide carbon source.It is because of portion but from fig. 9, it can be seen that the total sugar content of experimental group is always less than blank control group
Point dendrobium polysaccharide be utilized by the microorganism as its own grow required for carbon source, 30 days experimental group and blank after fermentation
It is 131mg/mL that control group total sugar content, which reaches maximum value,.
The comparison of 6.2 total amino acid contents
As seen from Figure 13, experimental group amino acid content amplitude of variation is bigger, and the 8th day in fermentation reaches maximum value
It for 1455 μ g/mL, begins to decline later, had a rising by a small margin again at the 24th day, this is because the fast fast-growing of preceding 8 days strains
It is long, a large amount of protease is generated, protein has been decomposed and has produced amino acid for growth.The amino acid content of blank control group
Change more stable, is always maintained at the trend slightly risen since fermentation the 4th day, reaching maximum value within the 20th day in fermentation is
1125 μ g/mL, decline by a small margin later, and experimental group amino acid content is higher than control group by 17.22% after fermentation in 30 days.
7, the variation of fermentation pH
As shown in Figure 14, pH value presentation falls before the variation tendency slightly risen afterwards in entire fermentation process, and the of fermentation
Reaching within 16 days minimum pH is 3.74.PH early period decline generates amino acid because of aerobic fermentation consumption protein, on mid-term is slight
It rises because of amino acid small molecule synthesising thalli protein, pH keeps inclined caused by the later period is metabolized out the organic acids such as lactic acid because of lactic acid bacteria intervention
It is acid.
In conclusion containing functional probiotics and its metabolite in dendrobium nobile flower prod, is removed in product and remain stone flower
Outside the nutritional ingredient of itself, also comprising multi-nutrients such as probiotics, dendrobium polysaccharide, protease, lipase, amylase, have
Remove the plurality of health care functions such as free radical, anti-aging, antibacterial anti-inflammatory, enhancing immunity of organism.Probiotics decomposes the microcosmic knot of stone flower
Structure, promotes the dissolution of polysaccharide component, and growth of probiotics, proliferation function realize the decomposition of stone flower institutional framework, promote stone
The release of dry measure used in former times flower polysaccharide, polysaccharide release rate improve 52.2%.It is dry using freeze-drying mode, utmostly remain stone flower
Volatile essential oil ingredient and probiotics, probiotics living body bacterium number can reach 8.5 × 106CFU/g.Probiotics dendrobium nobile flower prod antioxygen
Change activity to be obviously improved, zymoprotein vigor significantly improves.
The above examples only illustrate the technical idea of the present invention, and this does not limit the scope of protection of the present invention, all
According to the technical idea provided by the invention, any changes made on the basis of the technical scheme each falls within the scope of the present invention
Within；The technology that the present invention is not directed to can be realized by the prior art.
Claims (9)
1. a kind of processing method of probiotics stone flower, which is characterized in that include following specific steps:
(1) fresh stone flower pretreatment: manually harvesting fresh stone flower, after sorting removal of impurities, the impurity such as clear water rinsed surfaces floating dust, 40
DEG C~50 DEG C under the conditions of, through 4-6 hours dry, remove surface moisture after, 4 DEG C of -10 DEG C of refrigerations are spare；
(2) fermentation substrate is deployed: take pretreated fresh stone flower, with atomizers spray clear water to flower surface, reach it is wet and
Agglomerating degree of not dripping is held, the moist heat sterilization 5min-10min at 110 DEG C~115 DEG C, hair can be filtered by being packed into 500-1000mL
Ferment bottle, is cooled to room temperature, leavening to be accessed；
(3) prepared by leavening: Ganoderma Lucidum UIM-135 being accessed potato dextrose broth, at 28 DEG C~30 DEG C, 150
Mycelium pellet is collected as leavening A in shaken cultivation 3~4 days under conditions of~180r/min；With milk containing 3%-5%, 4~8%
The MS culture medium of oligofructose carries out Liquid static culture to lactobacillus acidophilus and Bifidobacterium at 30 DEG C~35 DEG C, take 15~
The bacterium solution of culture in 20 hours is as leavening B；
(4) stone flower is fermented: stone flower zymotechnique uses two sections of fermentation methods, and leading portion fermentation carries out aerobic hair using leavening A
Ferment, back segment fermentation carry out anaerobic fermentation using leavening B；
(5) probiotics stone flower is dry: the stone flower fermentation material after taking back segment to ferment 5-15 days, paves on pallet, thickness
After 0.5-1.0cm, -25 DEG C of pre-freeze 5h, 18h is freeze-dried under the conditions of -40 DEG C, gained finished product is sub-packed in vacuum seal bag, i.e.,
Obtain the probiotics dendrobium nobile flower prod.
2. a kind of processing method of probiotics stone flower according to claim 1, it is characterised in that: the fresh stone flower
For the fresh stone flower in the petal phase, petal is not yet unfolded, is wrapped in petal, and 1~4h is picked before blooming, and after picking
Pretreatment in 0.5~2h.
3. a kind of processing method of probiotics stone flower according to claim 1, it is characterised in that: the fresh stone flower
Be derived from dendrobium candidum, HERBA DENDROBII, dendrobium hancockii Rolfe, weight lip dendrobium nobile, the red dendrobium nobile of larynx, long Soviet Union's dendrobium nobile, Dendrobium thyrsiflorum, wing stalk dendrobium nobile,
One of purple dendrobium, Dendrobium Moniliforme, Dendrobium chrysanthum, Dendrobium Chrysotoxum Lindl, yellow larynx dendrobium nobile, Falconer Dendrobium are a variety of.
4. a kind of processing method of probiotics stone flower according to claim 1, it is characterised in that: the leading portion fermentation
The specific steps are have been charged into pretreatment after stone flower filter in fermentation flask by 10%~15% inoculum concentration access UIM-135
Mycelium pellet after gently stirring evenly, is placed in 28 DEG C~30 DEG C ferment at constant temperature, and daily fermented bottle suction filtration mouth pressure is passed through filtrated air 1
~3 times, to maintain aerobic condition；After continuing fermentation 5-15 days, after the visible UIM-135 mycelia of naked eyes generates between stone flower, leading portion
Fermentation ends.
5. a kind of processing method of probiotics stone flower according to claim 1, it is characterised in that: the back segment fermentation
The specific steps are in leading portion fermentation period terminal, continue with 5%~10% inoculum concentration, sprinkling access leavening B shakes up,
After fermented bottle suction filtration mouth extraction air, 30 DEG C under confined conditions~40 DEG C anaerobic fermentation 5~15 days；Stirring 2 daily in first 5 days~
It 3 times, stirs 1~5 minute, is not stirred for later every time, standing for fermentation is to terminal.
6. a kind of processing method of probiotics stone flower according to claim 1, it is characterised in that: the leavening B
The total concentration of middle thallus is 5 × 104~5The ratio between viable count of CFU/mL, lactobacillus acidophilus and Bifidobacterium is 1~10:1.
7. a kind of probiotics stone flower of the preparation of the processing method as described in claim any one of 1-6, it is characterised in that: include
The prebiotic bacterium number of living body is 8.5 × 104~6CFU/g, total starches content be 90~131mg/mL, total amino acid content be 1125~
1455 μ g/mL, total phenol content are 170~185mg/L, pH value 3.74~3.87.
8. probiotics stone flower according to claim 7, it is characterised in that: it also include four kinds of functional enzymes, it is respectively super
Superoxide dismutase, protease, amylase and lipase.
9. probiotics stone flower according to claim 8, it is characterised in that: the superoxide dismutase enzyme activity is
6500~8500U/L；The enzyme activity of protease is 1840.8~2490.4U/L；Amylase enzyme activity is 325~334.6U/L；Fat
Enzyme enzyme activity is 100~450U/L.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111357841A (en) * | 2020-03-26 | 2020-07-03 | 湖南营养树生物科技有限公司 | Preparation method of probiotics white tea for resisting helicobacter pylori |
| TWI737159B (en) * | 2020-02-07 | 2021-08-21 | 蕾迪詩生物科技股份有限公司 | Preparation method of Dendrobium spp. anti-oxidant extract |
| CN113966832A (en) * | 2020-07-22 | 2022-01-25 | 安琪纽特股份有限公司 | Dendrobium nobile fermentation product and preparation method and application thereof |
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|---|---|---|---|---|
| CN105146509A (en) * | 2015-07-28 | 2015-12-16 | 昆明宝成生物科技有限公司 | Novel probiotic dendrobium enzyme and production technology thereof |
| CN105707628A (en) * | 2016-03-11 | 2016-06-29 | 三株福尔制药有限公司 | Probiotic fermentation dendrobium officinale protocorm composition and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105146509A (en) * | 2015-07-28 | 2015-12-16 | 昆明宝成生物科技有限公司 | Novel probiotic dendrobium enzyme and production technology thereof |
| CN105707628A (en) * | 2016-03-11 | 2016-06-29 | 三株福尔制药有限公司 | Probiotic fermentation dendrobium officinale protocorm composition and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI737159B (en) * | 2020-02-07 | 2021-08-21 | 蕾迪詩生物科技股份有限公司 | Preparation method of Dendrobium spp. anti-oxidant extract |
| CN111357841A (en) * | 2020-03-26 | 2020-07-03 | 湖南营养树生物科技有限公司 | Preparation method of probiotics white tea for resisting helicobacter pylori |
| CN113966832A (en) * | 2020-07-22 | 2022-01-25 | 安琪纽特股份有限公司 | Dendrobium nobile fermentation product and preparation method and application thereof |
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