CN109511679A - A kind of method that chemical and biological pesticide prevents and treats vegetables and crops root-knot nematodes - Google Patents

A kind of method that chemical and biological pesticide prevents and treats vegetables and crops root-knot nematodes Download PDF

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Publication number
CN109511679A
CN109511679A CN201710837023.1A CN201710837023A CN109511679A CN 109511679 A CN109511679 A CN 109511679A CN 201710837023 A CN201710837023 A CN 201710837023A CN 109511679 A CN109511679 A CN 109511679A
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China
Prior art keywords
phoxim
vegetables
root
crops
biological pesticide
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CN201710837023.1A
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Chinese (zh)
Inventor
张志高
夏长松
王通岭
舒畅
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ZHONGKAI AGRICULTURAL CHEMICAL Ltd XINYI
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ZHONGKAI AGRICULTURAL CHEMICAL Ltd XINYI
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Priority to CN201710837023.1A priority Critical patent/CN109511679A/en
Publication of CN109511679A publication Critical patent/CN109511679A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
    • A01N25/28—Microcapsules or nanocapsules
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/14—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing aromatic radicals
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of chemistry and biological pesticide prevention and treatment vegetables and the methods of crops root-knot nematodes, are related to the technical field of root knot nematode control, the method being used cooperatively using chemical pesticide and biological pesticide.The present invention provides a kind of efficient, vegetables of safety, green, economy and crops root-knot nematodes control methods, can effectively kill vegetables and crop growth phase root-knot nematode；And the method for the invention is easy to use, vegetables and crops be can be improved to nutriment and utilization rate of fertilizer, vegetables and crops immunity and disease resistance is improved, improve vegetables and crops quality, increase yield significantly, long-time service can improve soil quality, achieve the purpose that green prevention and control.

Description

A kind of method that chemical and biological pesticide prevents and treats vegetables and crops root-knot nematodes
Technical field:
The present invention relates to a kind of chemistry and biological pesticide prevention and treatment vegetables and the methods of crops root-knot nematodes, belong to plant guarantor Protect technical field.
Background technique:
Root-knot nematode (Meloidogyne) is a kind of omnivorousness plant pathogeny line insect of height specialized form.Root knot nematode disease One of vegetable melon and fruit and the main disease of crops, occur root-knot nematode endanger field can underproduction 15-25%, serious field is very Up to 80% or more, the serious production and farmers' income for affecting vegetable melon and fruit and crops.
The generation of root knot nematode disease is usually also accompanied by plant soil-borne diseases, and composition is multiple to be infected, and as a result causes more serious Loss.Plant soil-borne diseases have that host types are more, resistance is strong, are difficult to the features such as preventing and treating.Root-knot nematode is prevented and treated for a long time With soil-borne disease still based on chemical prevention.However the chemical pesticide of tradition prevention and treatment root knot nematode disease has toxicity high, effective Phase is short, needs the shortcomings that frequently using, however chemical pesticide excessively apply can bring a series of ecological and social concern, such as works as Preceding 3R problem, the wildness once again of disease pest and weed, direct or indirect jeopardizes non-target organism, contaminated soil, water, atmosphere etc. Environmental factor and bioconcentration finally endanger the health of the mankind oneself.With the development of economy, people's living standard It improves, food-safety problem is increasingly paid close attention to by people, and green, organic agricultural products are also increasingly favored by people.But Be that preferable effect is all not achieved in current single nematode killing agent, need multiple medication, cause economic cost height and and pesticide residue it is high, Unfavorable food safety；Long-time service also results in the problem of soil hardening.And microbial pesticide is affected by environment larger, to above the average age for marriage Imago killing rate is not high.And therefore prevent and treat root knot nematode disease, it is necessary to which selection low toxicity, the chemical pesticide of low-residual and biological pesticide are matched It closes and uses, do not only reach and effect is effectively prevented to nematode, moreover it is possible to realize food safety, increasing both production and income, the effect for improveing soil. Therefore the present invention has developed a kind of method that chemical and biological pesticide prevents and treats vegetables and crops root-knot nematodes, solves current anti- Control the problem of root-knot nematode.
Summary of the invention:
To solve the problems, such as that preventing and treating root knot nematode disease at present exists, the present invention provides a kind of chemical pesticide microcapsule suspending agents The method for preventing and treating vegetables and crops root-knot nematodes with biological pesticide.
Purpose to realize the present invention, technical scheme is as follows:
1, a kind of method of chemistry and biological pesticide prevention and treatment vegetables and crops root-knot nematodes, utilizes chemical pesticide phoxim micro-capsule Suspending agent is in the transplantation of seedlings last stage；Using microbial pesticide line go out (waxy Bacillus) after transplantation of seedlings stage carry out.
2, preferably, chemical pesticide phoxim is microcapsule suspending agent dosage form, using water as carrier in process, without appointing in product What organic solvent, effective component phoxim is placed among micro-capsule, the chance photodegradation of phoxim can be effectively controlled, make phoxim Photodegradation rate substantially reduces, effective component slow release, and application preventive effect phase is 30-40 days.
3, further, phoxim microcapsule suspending agent effective component phoxim content is 30%, is watered according to the ratio of 1:200 dilute It releases, medical fluid dosage 1000-2000 liter/mu, the preceding cave of transplantation of seedlings, which is applied or filled, applies.
4, biological pesticide line go out in contained living microorganism bacterium, be waxy Bacillus (Bacillus cereus).
5, preferably, biological pesticide Xian Mie manufacturer is Xinyi Zhongkai Agricultural Chemical Co., Ltd, viable bacteria wax in product Bacillus total concentration is 1 × 109~1 × 1010cfu/ml。
6, further, line goes out dilutes according to 1:200, after transplantation of seedlings 10 days pouring roots or with chemical pesticide simultaneously mix apply carry out cave It applies or fills and apply, medical fluid dosage 1000-2000 liter/mu.
The mechanism for the integrated control root knot nematode disease that inventor provides are as follows: chemical pesticide phoxim microcapsule suspending agent is a kind of Low toxicity, the lasting period is longer, slow-release organophosphorus insecticide, have tag, stomach toxicity and fumigation action, no systemic action, to nematode Adult, larva have killing effect.Biological pesticide line, which goes out, forms dominant microflora in plant rhizosphere, has very strong suppression to nematode larval Production is used, so that the root-knot nematode of obligatory parasitism is dead due to can not infect plant root；The disease-resistant function of plant itself is excited, Improve plant immune ability.
Chemical pesticide phoxim microcapsule suspending agent is good to root-knot nematode adult and larva killing effect, can be in 30- after application The nematode in soil is persistently killed in 40 days；Biological pesticide line, which goes out, to be killed nematode larval and inhibitory effect is preferable, and can be secreted It is absorbed beneficial to rhzomorph substance by vegetables and crops, improves the quality and yield of vegetables and crops.Two kinds of pesticide cooperations make With nematode can be killed in vegetables and effectively in the crop growth phase, greatly reduces root-knot nematode and vegetables are infected.
Specific embodiment:
The preparation of 1 chemical pesticide phoxim microcapsule suspending agent of case study on implementation
30% phoxim suspending agent, which is blended into the water of different proportion, to be diluted, and selection is prepared most at the cloudy day or at dusk It is good.
2 biological pesticide line of case study on implementation goes out preparation
The dilution in the water for be blended into different proportion of going out of biological pesticide line obtains medical fluid.
Implement test in the field of the present invention of case study on implementation 3
Above-mentioned prevention and treatment root knot nematode disease method field control effectiveness test is as follows:
Test objective: field vegetables pharmacodynamic test is carried out to root-knot nematode, is divided according to the test result specifically obtained Analysis, to confirm its real control efficiency.
Experimental condition
Subjects: root-knot nematode
Test vegetable variety: greenhouse tomato, cucumber
Test medicine: 30% phoxim microcapsule suspending agent, line goes out, and (waxy Bacillus total concentration is 1 × 109~1 × 1010cfu/ml)；Blank control: clear water
Content of the test
The same greenhouse of tomato containing root-knot nematode is divided into different community block, each four are handled, totally eight block of cells. Phoxim, which dilutes 200 times of liquid+lines and goes out, dilutes 200 times of liquid (T1)；Phoxim, which dilutes 300 times of liquid+lines and goes out, dilutes 200 times of liquid (T2)； Phoxim, which dilutes 400 times of liquid+lines and goes out, dilutes 200 times of liquid (T3)；Clear water blank control (CK), each block of cells processing are repeated 2 times, Totally eight processing.
The same greenhouse of cucumber containing root-knot nematode is divided into different community block, each four are handled, totally eight block of cells.It is pungent Sulphur phosphorus, which dilutes 200 times of liquid+lines and goes out, dilutes 200 times of liquid (T1)；Phoxim, which dilutes 300 times of liquid+lines and goes out, dilutes 200 times of liquid (T2)；It is pungent Sulphur phosphorus, which dilutes 400 times of liquid+lines and goes out, dilutes 200 times of liquid (T3)；Clear water blank control (CK), each block of cells processing are repeated 2 times, altogether Eight processing.
Before transplantation of seedlings
Will in greenhouse tomato, cucumber field, by 1 Chinese medicine liquid of case study on implementation spray into cave carry out cave apply, will be western Earthing immediately after red persimmon, cucumber seedling are transplanted.
After transplantation of seedlings
10 days after tomato, cucumber seedling transplanting, the microbial inoculum prepared in case study on implementation 2 is added dilute in the water of different proportion It releases, pouring root is carried out to tomato, cucumber seedling.
Application front and back soil sampling
Before application, 15 days after application, 60 days after application, sampled after carrying out medicine to 8 field soil respectively using five point sampling, And nematode separation is carried out to soil sample using centrifugal separation.
Observation method
Observation living nematodes are carried out using anatomical lens to the sample after eight centrifuge separations respectively and are counted.
Drug effect comparative approach
Compare the nematode adult to survive in 8 samples and larva sum.
Test data record:
Tomato field data:
Cucumber field data:
Test result analysis: phoxim, which dilutes 200 times of liquid+lines and goes out, dilutes 200 times of liquid (T1)；300 times of liquid of phoxim dilution+ Line, which goes out, dilutes 200 times of liquid (T2), and two kinds of processing scheme preventive effects are all preferable, and nemic death rate is all higher, and it is longer to be administered preventive effect phase.
In place of particularly advantageous effect of the invention: the present invention solves length during vegetables and crops root-knot nematodes prevention and treatment Phase uses high pesticide residue caused by chemical pesticide to reduce vegetable and fruit and crops quality decline, and the present invention provides a kind of high Effect, safety, the vegetable and fruit of green economy and crops root-knot nematodes control method, can effectively kill root-knot nematode.And this Invention the method is easy to use, can guarantee vegetable growth needed nutrient matter, improves vegetable and fruit and crop yield, long Phase use can improve soil quality.

Claims (8)

1. a kind of method of chemistry and biological pesticide prevention and treatment vegetables and crops root-knot nematodes, which is characterized in that utilize chemical agriculture In vegetables and crops different stages of growth and nematode phase cooperation, which occurs, for encapsulated form made of medicine and biological pesticide live bacteria agent makes With obtaining to a kind of green of root-knot nematode, efficient control method.
2. the method according to claim 1, wherein chemical pesticide includes phoxim, fosthiazate, chlopyrifos, thiophene Worm piperazine, ethoprop, gram line pellet, nemamort, avermectin, carbofuran, Aldicarb etc.；Biological pesticide includes bacillus subtilis Bacterium, waxy Bacillus, bacillus amyloliquefaciens, bacillus thuringiensis, trichoderma viride, streptomycete, Pasteurella, unwrapping wire Bacterium, green muscardine fungus, muscardine etc..Vegetables and crops include tomato, cucumber, cucurbita pepo, Chinese yam, Radix Notoginseng, wheat, corn, water Rice, sweet potato, pepper, citrus, apple, banana, strawberry etc..
3. according to the method described in claim 2, it is characterized in that, phoxim and other chemical pesticides are prepared into microcapsule suspending agent Dosage form, the effective component for preventing and treating root-knot nematode are 1-50%.
4. according to the method described in claim 3, it is characterized in that, preferably, chemical pesticide phoxim is low-toxin farm chemicals, agent Type is microcapsule suspending agent dosage form, and effective component phoxim is placed among micro-capsule, the chance photodegradation of phoxim can be effectively controlled, Substantially reduce phoxim photodegradation rate, effective ingredient slow release, the continuous and effective phase is greater than 30 days.
5. according to the method described in claim 3, it is characterized in that, phoxim microcapsule suspending agent effective component phoxim content is 30%, when 30% phoxim microcapsule suspending agent it is a kind of efficiently, the water baseization product of wide spectrum, high technology content, without any in product Organic solvent reduces environmental pollution.
6. according to the method described in claim 2, it is characterized in that, preferably, biological pesticide line go out in contained living body strain, For waxy Bacillus (Bacillus cereus), Chinese microorganism strain preservation management is preserved on June 18th, 2009 Committee's common micro-organisms center, culture presevation number are CGMCC NO.3128.
7. according to the method described in claim 6, it is characterized in that, biological pesticide Xian Mie manufacturer is triumphant agriculturalization in Xinyi Work Co., Ltd, guaranteed vegetable growth needed nutrient matter is contained in product, and long-time service can improve soil quality；It is living in product Bacterium waxy Bacillus total concentration is 1 × 109~1 × 1010cfu/ml。
8. the method according to claim 1, wherein phoxim microcapsule suspending agent carries out cave in the transplantation of seedlings last stage Apply or do after transplanting root water application；Biological pesticide line goes out to mix with chemical pesticide arbitrary proportion and apply after transplantation of seedlings, can be used It pours, drip irrigation method application；Before transplantation of seedlings, biological pesticide line can be gone out dilution and phoxim microcapsule suspending agent dilution After mixing, it is administered using pouring, drip irrigation method.Making preferably, phoxim microcapsule suspending agent is watered according to the ratio of 1:200 Object is transplanted preceding cave and is applied, medical fluid usage amount 1000-2000 liter/mu；Line goes out to be diluted according to 1:200,10 days progress pouring roots after transplantation of seedlings, Medical fluid usage amount 1000-2000 liter/mu.
CN201710837023.1A 2017-09-19 2017-09-19 A kind of method that chemical and biological pesticide prevents and treats vegetables and crops root-knot nematodes Pending CN109511679A (en)

Priority Applications (1)

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CN201710837023.1A CN109511679A (en) 2017-09-19 2017-09-19 A kind of method that chemical and biological pesticide prevents and treats vegetables and crops root-knot nematodes

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